ADP binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 148 |
| AccB1I | GGYRCC | 3 cut(s) | 148, 756, 1473 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 698, 1527 |
| AciI | CCGC | 4 cut(s) | 11, 153, 1477, 1484 |
| AclWI | GGATC | 2 cut(s) | 522, 1545 |
| AcoI | YGGCCR | 1 cut(s) | 376 |
| AcsI | RAATTY | 4 cut(s) | 340, 445, 778, 1373 |
| AcuI | CTGAAG | 2 cut(s) | 593, 701 |
| AdeI | CACNNNGTG | 1 cut(s) | 571 |
| AfaI | GTAC | 5 cut(s) | 150, 801, 875, 1072, 1567 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 698, 965, 1097, 1412, 1483, 1527 |
| AflII | CTTAAG | 1 cut(s) | 773 |
| AflIII | ACRYGT | 1 cut(s) | 397 |
| AgsI | TTSAA | 9 cut(s) | 26, 73, 784, 813, 821, 947, 956, 1246, 1252 |
| AjnI | CCWGG | 3 cut(s) | 119, 163, 1570 |
| AjuI | GAANNNNNNNTTGG | 1 cut(s) | 1589 |
| AluBI | AGCT | 7 cut(s) | 199, 423, 634, 772, 854, 892, 1393 |
| AluI | AGCT | 7 cut(s) | 199, 423, 634, 772, 854, 892, 1393 |
| Alw21I | GWGCWC | 2 cut(s) | 601, 894 |
| Alw26I | GTCTC | 2 cut(s) | 297, 1563 |
| Alw44I | GTGCAC | 1 cut(s) | 597 |
| AlwI | GGATC | 2 cut(s) | 522, 1545 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 724 |
| AoxI | GGCC | 3 cut(s) | 376, 681, 967 |
| ApaLI | GTGCAC | 1 cut(s) | 597 |
| ApeKI | GCWGC | 2 cut(s) | 242, 854 |
| ApoI | RAATTY | 4 cut(s) | 340, 445, 778, 1373 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 271, 303, 1382, 1414 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 951 |
| Asp718I | GGTACC | 1 cut(s) | 148 |
| AspLEI | GCGC | 1 cut(s) | 226 |
| AspS9I | GGNCC | 1 cut(s) | 681 |
| AsuHPI | GGTGA | 4 cut(s) | 557, 1159, 1289, 1502 |
| AsuII | TTCGAA | 1 cut(s) | 487 |
| BaeGI | GKGCMC | 3 cut(s) | 105, 601, 626 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 108, 141 |
| BalI | TGGCCA | 1 cut(s) | 378 |
| BanI | GGYRCC | 3 cut(s) | 148, 756, 1473 |
| BanII | GRGCYC | 1 cut(s) | 894 |
| BauI | CACGAG | 1 cut(s) | 405 |
| BbsI | GAAGAC | 3 cut(s) | 378, 944, 1325 |
| Bbv12I | GWGCWC | 2 cut(s) | 601, 894 |
| BbvCI | CCTCAGC | 1 cut(s) | 846 |
| BbvI | GCAGC | 2 cut(s) | 254, 841 |
| BceAI | ACGGC | 2 cut(s) | 230, 744 |
| BciT130I | CCWGG | 3 cut(s) | 121, 165, 1572 |
| BclI | TGATCA | 1 cut(s) | 1189 |
| BcoDI | GTCTC | 2 cut(s) | 297, 1563 |
| BfaI | CTAG | 3 cut(s) | 108, 887, 1512 |
| BfmI | CTRYAG | 1 cut(s) | 1063 |
| BfrI | CTTAAG | 1 cut(s) | 773 |
| BisI | GCNGC | 3 cut(s) | 243, 855, 1477 |
| BlsI | GCNGC | 3 cut(s) | 244, 856, 1478 |
| BmcAI | AGTACT | 1 cut(s) | 801 |
| Bme1390I | CCNGG | 3 cut(s) | 121, 165, 1572 |
| BmgT120I | GGNCC | 1 cut(s) | 681 |
| BmiI | GGNNCC | 4 cut(s) | 150, 268, 758, 1475 |
| BmrFI | CCNGG | 3 cut(s) | 121, 165, 1572 |
| BpiI | GAAGAC | 3 cut(s) | 378, 944, 1325 |
| Bpu10I | CCTNAGC | 1 cut(s) | 846 |
| Bpu14I | TTCGAA | 1 cut(s) | 487 |
| BpuEI | CTTGAG | 3 cut(s) | 1238, 1346, 1451 |
| BsaJI | CCNNGG | 4 cut(s) | 120, 246, 270, 379 |
| BsaWI | WCCGGW | 1 cut(s) | 894 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 698, 965, 1097, 1412, 1483, 1527 |
| Bse1I | ACTGG | 1 cut(s) | 61 |
| BseBI | CCWGG | 3 cut(s) | 121, 165, 1572 |
| BseDI | CCNNGG | 4 cut(s) | 120, 246, 270, 379 |
| BseGI | GGATG | 4 cut(s) | 12, 256, 1286, 1615 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 698, 965, 1097, 1412, 1483, 1527 |
| BseMII | CTCAG | 3 cut(s) | 216, 837, 906 |
| BseNI | ACTGG | 1 cut(s) | 61 |
| BseRI | GAGGAG | 2 cut(s) | 764, 810 |
| BseSI | GKGCMC | 3 cut(s) | 105, 601, 626 |
| BseXI | GCAGC | 2 cut(s) | 254, 841 |
| BseYI | CCCAGC | 2 cut(s) | 998, 1520 |
| BsgI | GTGCAG | 2 cut(s) | 338, 593 |
| BshFI | GGCC | 3 cut(s) | 378, 683, 969 |
| BshNI | GGYRCC | 3 cut(s) | 148, 756, 1473 |
| BsiHKAI | GWGCWC | 2 cut(s) | 601, 894 |
| BsiSI | CCGG | 1 cut(s) | 895 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 698, 965, 1097, 1412, 1483, 1527 |
| BsmAI | GTCTC | 2 cut(s) | 297, 1563 |
| BsmI | GAATGC | 2 cut(s) | 1116, 1196 |
| BsnI | GGCC | 3 cut(s) | 378, 683, 969 |
| Bsp119I | TTCGAA | 1 cut(s) | 487 |
| Bsp1286I | GDGCHC | 4 cut(s) | 105, 601, 626, 894 |
| Bsp1407I | TGTACA | 1 cut(s) | 1565 |
| Bsp143I | GATC | 4 cut(s) | 514, 861, 1189, 1550 |
| Bsp19I | CCATGG | 1 cut(s) | 379 |
| BspACI | CCGC | 4 cut(s) | 11, 153, 1477, 1484 |
| BspANI | GGCC | 3 cut(s) | 378, 683, 969 |
| BspCNI | CTCAG | 3 cut(s) | 215, 838, 907 |
| BspLI | GGNNCC | 4 cut(s) | 150, 268, 758, 1475 |
| BspPI | GGATC | 2 cut(s) | 522, 1545 |
| BspT104I | TTCGAA | 1 cut(s) | 487 |
| BspT107I | GGYRCC | 3 cut(s) | 148, 756, 1473 |
| BspTI | CTTAAG | 1 cut(s) | 773 |
| BsrGI | TGTACA | 1 cut(s) | 1565 |
| BsrI | ACTGG | 1 cut(s) | 61 |
| BssECI | CCNNGG | 4 cut(s) | 120, 246, 270, 379 |
| BssMI | GATC | 4 cut(s) | 514, 861, 1189, 1550 |
| BssSI | CACGAG | 1 cut(s) | 405 |
| BssT1I | CCWWGG | 2 cut(s) | 270, 379 |
| Bst2BI | CACGAG | 1 cut(s) | 405 |
| Bst2UI | CCWGG | 3 cut(s) | 121, 165, 1572 |
| Bst4CI | ACNGT | 2 cut(s) | 540, 1331 |
| Bst6I | CTCTTC | 3 cut(s) | 21, 567, 834 |
| BstAFI | CTTAAG | 1 cut(s) | 773 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1202 |
| BstAUI | TGTACA | 1 cut(s) | 1565 |
| BstBI | TTCGAA | 1 cut(s) | 487 |
| BstC8I | GCNNGC | 1 cut(s) | 967 |
| BstDEI | CTNAG | 6 cut(s) | 202, 587, 791, 846, 915, 1183 |
| BstDSI | CCRYGG | 2 cut(s) | 246, 379 |
| BstF5I | GGATG | 4 cut(s) | 12, 256, 1286, 1615 |
| BstHHI | GCGC | 1 cut(s) | 226 |
| BstKTI | GATC | 4 cut(s) | 517, 864, 1192, 1553 |
| BstMAI | GTCTC | 2 cut(s) | 297, 1563 |
| BstMBI | GATC | 4 cut(s) | 514, 861, 1189, 1550 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 851, 1202 |
| BstNI | CCWGG | 3 cut(s) | 121, 165, 1572 |
| BstNSI | RCATGY | 1 cut(s) | 401 |
| BstSCI | CCNGG | 3 cut(s) | 119, 163, 1570 |
| BstSFI | CTRYAG | 1 cut(s) | 1063 |
| BstSLI | GKGCMC | 3 cut(s) | 105, 601, 626 |
| BstV1I | GCAGC | 2 cut(s) | 254, 841 |
| BstV2I | GAAGAC | 3 cut(s) | 378, 944, 1325 |
| BstX2I | RGATCY | 2 cut(s) | 514, 1550 |
| BstYI | RGATCY | 2 cut(s) | 514, 1550 |
| BsuRI | GGCC | 3 cut(s) | 378, 683, 969 |
| BtgI | CCRYGG | 2 cut(s) | 246, 379 |
| BtsCI | GGATG | 4 cut(s) | 12, 256, 1286, 1615 |
| BtsIMutI | CAGTG | 2 cut(s) | 54, 912 |
| Cac8I | GCNNGC | 1 cut(s) | 967 |
| CaiI | CAGNNNCTG | 1 cut(s) | 724 |
| CfoI | GCGC | 1 cut(s) | 226 |
| Cfr13I | GGNCC | 1 cut(s) | 681 |
| CseI | GACGC | 1 cut(s) | 338 |
| Csp6I | GTAC | 5 cut(s) | 149, 800, 874, 1071, 1566 |
| CviAII | CATG | 6 cut(s) | 380, 398, 569, 685, 962, 1240 |
| CviQI | GTAC | 5 cut(s) | 149, 800, 874, 1071, 1566 |
| DdeI | CTNAG | 6 cut(s) | 202, 587, 791, 846, 915, 1183 |
| DpnI | GATC | 4 cut(s) | 516, 863, 1191, 1552 |
| DpnII | GATC | 4 cut(s) | 514, 861, 1189, 1550 |
| DraIII | CACNNNGTG | 1 cut(s) | 571 |
| EaeI | YGGCCR | 1 cut(s) | 376 |
| Eam1104I | CTCTTC | 3 cut(s) | 21, 567, 834 |
| EarI | CTCTTC | 3 cut(s) | 21, 567, 834 |
| EciI | GGCGGA | 2 cut(s) | 26, 1499 |
| Ecl136II | GAGCTC | 1 cut(s) | 892 |
| Eco130I | CCWWGG | 2 cut(s) | 270, 379 |
| Eco24I | GRGCYC | 1 cut(s) | 894 |
| Eco32I | GATATC | 2 cut(s) | 664, 1509 |
| Eco53kI | GAGCTC | 1 cut(s) | 892 |
| Eco57I | CTGAAG | 2 cut(s) | 593, 701 |
| EcoICRI | GAGCTC | 1 cut(s) | 892 |
| EcoRI | GAATTC | 1 cut(s) | 1373 |
| EcoRII | CCWGG | 3 cut(s) | 119, 163, 1570 |
| EcoRV | GATATC | 2 cut(s) | 664, 1509 |
| EcoT14I | CCWWGG | 2 cut(s) | 270, 379 |
| EcoT38I | GRGCYC | 1 cut(s) | 894 |
| ErhI | CCWWGG | 2 cut(s) | 270, 379 |
| FaeI | CATG | 6 cut(s) | 383, 401, 572, 688, 965, 1243 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 77, 109 |
| FatI | CATG | 6 cut(s) | 379, 397, 568, 684, 961, 1239 |
| FauI | CCCGC | 1 cut(s) | 160 |
| FauNDI | CATATG | 1 cut(s) | 1526 |
| FbaI | TGATCA | 1 cut(s) | 1189 |
| Fnu4HI | GCNGC | 3 cut(s) | 243, 855, 1477 |
| FokI | GGATG | 3 cut(s) | 19, 263, 1273 |
| FriOI | GRGCYC | 1 cut(s) | 894 |
| Fsp4HI | GCNGC | 3 cut(s) | 243, 855, 1477 |
| FspBI | CTAG | 3 cut(s) | 108, 887, 1512 |
| GlaI | GCGC | 1 cut(s) | 225 |
| GluI | GCNGC | 3 cut(s) | 243, 855, 1477 |
| GsaI | CCCAGC | 2 cut(s) | 1002, 1524 |
| HaeIII | GGCC | 3 cut(s) | 378, 683, 969 |
| HapII | CCGG | 1 cut(s) | 895 |
| HgaI | GACGC | 1 cut(s) | 338 |
| HhaI | GCGC | 1 cut(s) | 226 |
| Hin1II | CATG | 6 cut(s) | 383, 401, 572, 688, 965, 1243 |
| Hin6I | GCGC | 1 cut(s) | 224 |
| HinP1I | GCGC | 1 cut(s) | 224 |
| HincII | GTYRAC | 3 cut(s) | 1005, 1082, 1234 |
| HindII | GTYRAC | 3 cut(s) | 1005, 1082, 1234 |
| HindIII | AAGCTT | 2 cut(s) | 632, 1391 |
| HinfI | GANTC | 8 cut(s) | 134, 331, 451, 548, 557, 721, 1310, 1433 |
| HpaII | CCGG | 1 cut(s) | 895 |
| HphI | GGTGA | 4 cut(s) | 557, 1159, 1289, 1502 |
| Hpy166II | GTNNAC | 7 cut(s) | 214, 599, 912, 1005, 1082, 1234, 1568 |
| Hpy188I | TCNGA | 8 cut(s) | 205, 297, 469, 720, 832, 1175, 1212, 1411 |
| Hpy188III | TCNNGA | 3 cut(s) | 108, 407, 746 |
| Hpy8I | GTNNAC | 7 cut(s) | 214, 599, 912, 1005, 1082, 1234, 1568 |
| HpyAV | CCTTC | 5 cut(s) | 8, 617, 725, 1416, 1481 |
| HpyCH4III | ACNGT | 2 cut(s) | 540, 1331 |
| HpyCH4V | TGCA | 5 cut(s) | 242, 355, 574, 599, 1205 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 851, 1202 |
| HpyF3I | CTNAG | 6 cut(s) | 202, 587, 791, 846, 915, 1183 |
| Hsp92II | CATG | 6 cut(s) | 383, 401, 572, 688, 965, 1243 |
| HspAI | GCGC | 1 cut(s) | 224 |
| KpnI | GGTACC | 1 cut(s) | 152 |
| Ksp22I | TGATCA | 1 cut(s) | 1189 |
| Kzo9I | GATC | 4 cut(s) | 514, 861, 1189, 1550 |
| LmnI | GCTCC | 3 cut(s) | 266, 897, 1500 |
| Lsp1109I | GCAGC | 2 cut(s) | 254, 841 |
| MaeI | CTAG | 3 cut(s) | 108, 887, 1512 |
| MaeIII | GTNAC | 4 cut(s) | 407, 978, 1104, 1277 |
| MalI | GATC | 4 cut(s) | 516, 863, 1191, 1552 |
| MboI | GATC | 4 cut(s) | 514, 861, 1189, 1550 |
| MboII | GAAGA | 9 cut(s) | 38, 378, 554, 640, 851, 944, 1325, 1505, 1553 |
| MfeI | CAATTG | 1 cut(s) | 674 |
| MflI | RGATCY | 2 cut(s) | 514, 1550 |
| MhlI | GDGCHC | 4 cut(s) | 105, 601, 626, 894 |
| MlsI | TGGCCA | 1 cut(s) | 378 |
| MluNI | TGGCCA | 1 cut(s) | 378 |
| MlyI | GAGTC | 5 cut(s) | 460, 557, 715, 1304, 1442 |
| MmeI | TCCRAC | 1 cut(s) | 1037 |
| Mox20I | TGGCCA | 1 cut(s) | 378 |
| MroXI | GAANNNNTTC | 1 cut(s) | 951 |
| MscI | TGGCCA | 1 cut(s) | 378 |
| MseI | TTAA | 6 cut(s) | 345, 425, 707, 774, 972, 1296 |
| Msp20I | TGGCCA | 1 cut(s) | 378 |
| MspCI | CTTAAG | 1 cut(s) | 773 |
| MspI | CCGG | 1 cut(s) | 895 |
| MspR9I | CCNGG | 3 cut(s) | 121, 165, 1572 |
| MunI | CAATTG | 1 cut(s) | 674 |
| Mva1269I | GAATGC | 2 cut(s) | 1116, 1196 |
| MvaI | CCWGG | 3 cut(s) | 121, 165, 1572 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 851, 1202 |
| NcoI | CCATGG | 1 cut(s) | 379 |
| NdeI | CATATG | 1 cut(s) | 1526 |
| NdeII | GATC | 4 cut(s) | 514, 861, 1189, 1550 |
| NlaIII | CATG | 6 cut(s) | 383, 401, 572, 688, 965, 1243 |
| NlaIV | GGNNCC | 4 cut(s) | 150, 268, 758, 1475 |
| NmuCI | GTSAC | 2 cut(s) | 407, 1277 |
| NspI | RCATGY | 1 cut(s) | 401 |
| NspV | TTCGAA | 1 cut(s) | 487 |
| PciI | ACATGT | 1 cut(s) | 397 |
| PctI | GAATGC | 2 cut(s) | 1116, 1196 |
| PdmI | GAANNNNTTC | 1 cut(s) | 951 |
| PfeI | GAWTC | 3 cut(s) | 134, 331, 557 |
| PflFI | GACNNNGTC | 1 cut(s) | 724 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 698, 1527 |
| PkrI | GCNGC | 3 cut(s) | 244, 856, 1478 |
| PleI | GAGTC | 5 cut(s) | 459, 556, 715, 1304, 1441 |
| PpsI | GAGTC | 5 cut(s) | 459, 556, 715, 1304, 1441 |
| PscI | ACATGT | 1 cut(s) | 397 |
| Psp124BI | GAGCTC | 1 cut(s) | 894 |
| Psp6I | CCWGG | 3 cut(s) | 119, 163, 1570 |
| PspFI | CCCAGC | 2 cut(s) | 998, 1520 |
| PspGI | CCWGG | 3 cut(s) | 119, 163, 1570 |
| PspN4I | GGNNCC | 4 cut(s) | 150, 268, 758, 1475 |
| PspPI | GGNCC | 1 cut(s) | 681 |
| PstNI | CAGNNNCTG | 1 cut(s) | 724 |
| PsuI | RGATCY | 2 cut(s) | 514, 1550 |
| PsyI | GACNNNGTC | 1 cut(s) | 724 |
| RsaI | GTAC | 5 cut(s) | 150, 801, 875, 1072, 1567 |
| RsaNI | GTAC | 5 cut(s) | 149, 800, 874, 1071, 1566 |
| SacI | GAGCTC | 1 cut(s) | 894 |
| SaqAI | TTAA | 6 cut(s) | 345, 425, 707, 774, 972, 1296 |
| SatI | GCNGC | 3 cut(s) | 243, 855, 1477 |
| Sau3AI | GATC | 4 cut(s) | 514, 861, 1189, 1550 |
| Sau96I | GGNCC | 1 cut(s) | 681 |
| ScaI | AGTACT | 1 cut(s) | 801 |
| SchI | GAGTC | 5 cut(s) | 460, 557, 715, 1304, 1442 |
| ScrFI | CCNGG | 3 cut(s) | 121, 165, 1572 |
| SduI | GDGCHC | 4 cut(s) | 105, 601, 626, 894 |
| SfcI | CTRYAG | 1 cut(s) | 1063 |
| SfuI | TTCGAA | 1 cut(s) | 487 |
| SmlI | CTYRAG | 4 cut(s) | 773, 1217, 1361, 1430 |
| SmoI | CTYRAG | 4 cut(s) | 773, 1217, 1361, 1430 |
| SsiI | CCGC | 4 cut(s) | 11, 153, 1477, 1484 |
| SspMI | CTAG | 3 cut(s) | 108, 887, 1512 |
| SstI | GAGCTC | 1 cut(s) | 894 |
| StyD4I | CCNGG | 3 cut(s) | 119, 163, 1570 |
| StyI | CCWWGG | 2 cut(s) | 270, 379 |
| TaaI | ACNGT | 2 cut(s) | 540, 1331 |
| TaqI | TCGA | 4 cut(s) | 454, 487, 745, 864 |
| TatI | WGTACW | 3 cut(s) | 799, 1070, 1565 |
| TauI | GCSGC | 1 cut(s) | 1479 |
| TfiI | GAWTC | 3 cut(s) | 134, 331, 557 |
| Tru1I | TTAA | 6 cut(s) | 345, 425, 707, 774, 972, 1296 |
| Tru9I | TTAA | 6 cut(s) | 345, 425, 707, 774, 972, 1296 |
| TscAI | CASTG | 2 cut(s) | 61, 919 |
| TseFI | GTSAC | 2 cut(s) | 407, 1277 |
| TseI | GCWGC | 2 cut(s) | 242, 854 |
| Tsp45I | GTSAC | 2 cut(s) | 407, 1277 |
| TspDTI | ATGAA | 2 cut(s) | 1154, 1429 |
| TspRI | CASTG | 2 cut(s) | 61, 919 |
| Tth111I | GACNNNGTC | 1 cut(s) | 724 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 698, 1527 |
| Vha464I | CTTAAG | 1 cut(s) | 773 |
| VneI | GTGCAC | 1 cut(s) | 597 |
| XapI | RAATTY | 4 cut(s) | 340, 445, 778, 1373 |
| XbaI | TCTAGA | 1 cut(s) | 107 |
| XceI | RCATGY | 1 cut(s) | 401 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1344 |
| XmnI | GAANNNNTTC | 1 cut(s) | 951 |
| XspI | CTAG | 3 cut(s) | 108, 887, 1512 |
| ZrmI | AGTACT | 1 cut(s) | 801 |