Rmu_sc0014112.1_g000002

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0014112.1
OG0000546
Physical Location & Seq
Reverse (-)
1564 .. 2573
1010 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0014112.1_g000002.1.cds

Sequence Viewer

Length: 906 bp
atgaacccacagcggccgccgccgccgccgccaccctatgtgttcctcagttccggaggttacgccatgggcaatctctacactgctctggttgaagagggcatacctacaattcgagataatgaaaagctggagttgggatctgggcacagctcagaagagaaggaaaaaatatttgagaagacgttgcttccacaactctgcacatcaattgaagaatcgaagcttgtgattgtcatcatttcaataaattatctgaattcgagctggtgcttggagcagcttgccaaggctgttgaatgcaacaaactaatactccctgttttcgatgatgtgcatccaagtgaactacgacttcaaaagggatcagttgcggaagctttctttaagcacgaaaaaggctttaaagacaacccagacaaggtgcgaaaatggagacgagttattaatggcgtagctgacttgactggatgggatttacaccatagcgatgaaacccaagttattgaagctattgttgaaagagtttttattgaattaaatgaacgtctactgggattatttctctactgctccctcttcccagagggttacgaattcaggaaacaagagttggttcaattgtggatagctcaaggttttgatatgggagagagtcccggagagacaatggaagatgccggcattgagcatttcaacactttggagagtgaaggcttttttgtgcgctccggaagagctgatttaaaagtagaatatgatgaggtgatcactatgcccttttatgtcacccccagcttcttctacaaagtaaatccttctaagctttcacttttggaaaacaattgctccggtgctaaatattgcagagttgtggatggcaagtttgatgaagcatcagaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

301

Amino Acids

34.2

Weight (kDa)

4.88

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000546)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700
malus_domestica MD14G1049300.v1.1
rosa_chinensis RchiOBHm_Chr4g0433201 RchiOBHm_Chr6g0255111 RchiOBHm_Chr7g0235301 RchiOBHm_Chr7g0235351 RchiOBHm_Chr7g0235361 RchiOBHm_Chr7g0235381
rosa_laevigata RLG00000001164 RLG00000013941
rosa_multiflora Rmu_co8331033.1_g000001 Rmu_co8369635.1_g000001 Rmu_sc0000957.1_g000010 Rmu_sc0003304.1_g000033 Rmu_sc0003304.1_g000041 Rmu_sc0003488.1_g000001 Rmu_sc0004529.1_g000036 Rmu_sc0004637.1_g000013 Rmu_sc0004657.1_g000070 Rmu_sc0004657.1_g000076 Rmu_sc0006578.1_g000004 Rmu_sc0011027.1_g000008 Rmu_sc0011027.1_g000010 Rmu_sc0013813.1_g000001 Rmu_sc0014112.1_g000001 Rmu_sc0014112.1_g000002 Rmu_sc0014112.1_g000004 Rmu_ssc0000011.1_g000040 Rmu_ssc0000295.1_g000011
rosa_roxburghii Rroxscaffold_2G00103040 Rroxscaffold_3G00226100 Rroxscaffold_3G00226120 Rroxscaffold_5G00374710 Rroxscaffold_5G00374720 Rroxscaffold_7G00200160 Rroxscaffold_7G00200190
rosa_rugosa Rorug05G0474400 Rorug05G0474400 Rorug06G0037400 Rorug06G0037500 Rorug06G0037600 Rorug06G0038300 Rorug06G0038300 Rorug06G0038400 Rorug06G0038500 Rorug07G0289800 Rorug07G0289900 Rorug07G0290000 Rorug07G0290000
rosa_samantha Rh4AG320500 Rh4AG320700 Rh4AG321100 Rh4DG324200 Rh4DG324700 Rh6BG067500 Rh6CG066600 Rh6CG066700 Rh6DG063600 Rh6DG063700 Rh6DG147600 Rh7AG444800 Rh7AG445000 Rh7BG416700 Rh7BG416800 Rh7CG465200 Rh7CG465300 Rh7CG465700 Rh7DG433600 Rh7DG433800
rosa_wichuraiana Rw0G008730 Rw0G008770 Rw4G027830 Rw7G036940 Rw7G036980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 550
AccIII TCCGGA 2 cut(s) 53, 731
AciI CCGC 7 cut(s) 13, 17, 20, 23, 26, 29, 374
AclWI GGATC 2 cut(s) 148, 373
AcoI YGGCCR 1 cut(s) 14
AcsI RAATTY 2 cut(s) 259, 596
AfiI CCNNNNNNNGG 1 cut(s) 421
AjuI GAANNNNNNNTTGG 2 cut(s) 596, 628
Alw26I GTCTC 2 cut(s) 430, 659
AlwI GGATC 2 cut(s) 148, 373
Aor13HI TCCGGA 2 cut(s) 53, 731
AoxI GGCC 1 cut(s) 14
ApeKI GCWGC 1 cut(s) 280
ApoI RAATTY 2 cut(s) 259, 596
AseI ATTAAT 1 cut(s) 447
AspLEI GCGC 1 cut(s) 729
AsuC2I CCSGG 1 cut(s) 660
AsuHPI GGTGA 2 cut(s) 778, 781
BaeGI GKGCMC 1 cut(s) 150
BarI GAAGNNNNNNTAC 2 cut(s) 87, 119
BbsI GAAGAC 1 cut(s) 188
BbvI GCAGC 1 cut(s) 292
BccI CCATC 2 cut(s) 465, 872
BclI TGATCA 1 cut(s) 768
BcnI CCSGG 1 cut(s) 660
BcoDI GTCTC 2 cut(s) 430, 659
BisI GCNGC 7 cut(s) 14, 17, 20, 23, 26, 29, 281
BlsI GCNGC 7 cut(s) 15, 18, 21, 24, 27, 30, 282
Bme1390I CCNGG 1 cut(s) 660
BmrFI CCNGG 1 cut(s) 660
BmrI ACTGGG 1 cut(s) 563
BmsI GCATC 2 cut(s) 346, 667
BmuI ACTGGG 1 cut(s) 563
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 1 cut(s) 152
BpuEI CTTGAG 1 cut(s) 618
BpuMI CCSGG 1 cut(s) 660
BsaBI GATNNNNATC 2 cut(s) 236, 336
BsaJI CCNNGG 2 cut(s) 66, 288
BsaWI WCCGGW 3 cut(s) 53, 731, 851
BsaXI ACNNNNNCTCC 4 cut(s) 300, 330, 833, 863
Bsc4I CCNNNNNNNGG 1 cut(s) 421
Bse118I RCCGGY 1 cut(s) 680
Bse1I ACTGG 2 cut(s) 472, 558
Bse8I GATNNNNATC 2 cut(s) 236, 336
BseAI TCCGGA 2 cut(s) 53, 731
BseDI CCNNGG 2 cut(s) 66, 288
BseGI GGATG 3 cut(s) 337, 476, 883
BseJI GATNNNNATC 2 cut(s) 236, 336
BseLI CCNNNNNNNGG 1 cut(s) 421
BseMII CTCAG 2 cut(s) 61, 168
BseNI ACTGG 2 cut(s) 472, 558
BseSI GKGCMC 1 cut(s) 150
BseX3I CGGCCG 1 cut(s) 14
BseXI GCAGC 1 cut(s) 292
BseYI CCCAGC 1 cut(s) 794
BsgI GTGCAG 1 cut(s) 187
Bsh1285I CGRYCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 16
BsiEI CGRYCG 1 cut(s) 17
BsiSI CCGG 5 cut(s) 54, 660, 681, 732, 852
BslFI GGGAC 1 cut(s) 642
BslI CCNNNNNNNGG 1 cut(s) 421
BsmAI GTCTC 2 cut(s) 430, 659
BsmBI CGTCTC 1 cut(s) 430
BsmFI GGGAC 1 cut(s) 642
BsmI GAATGC 1 cut(s) 305
BsnI GGCC 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 150
Bsp13I TCCGGA 2 cut(s) 53, 731
Bsp143I GATC 3 cut(s) 140, 365, 768
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 7 cut(s) 13, 17, 20, 23, 26, 29, 374
BspANI GGCC 1 cut(s) 16
BspCNI CTCAG 2 cut(s) 60, 167
BspEI TCCGGA 2 cut(s) 53, 731
BspPI GGATC 2 cut(s) 148, 373
BspQI GCTCTTC 1 cut(s) 730
BsrFI RCCGGY 1 cut(s) 680
BsrI ACTGG 2 cut(s) 472, 558
BssAI RCCGGY 1 cut(s) 680
BssECI CCNNGG 2 cut(s) 66, 288
BssMI GATC 3 cut(s) 140, 365, 768
BssT1I CCWWGG 2 cut(s) 66, 288
Bst6I CTCTTC 4 cut(s) 90, 153, 584, 730
BstC8I GCNNGC 2 cut(s) 285, 682
BstDEI CTNAG 3 cut(s) 47, 154, 822
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 3 cut(s) 337, 476, 883
BstHHI GCGC 1 cut(s) 729
BstKTI GATC 3 cut(s) 143, 368, 771
BstMAI GTCTC 2 cut(s) 430, 659
BstMBI GATC 3 cut(s) 140, 365, 768
BstMCI CGRYCG 1 cut(s) 17
BstMWI GCNNNNNNNGC 4 cut(s) 19, 22, 25, 28
BstSCI CCNGG 1 cut(s) 658
BstSLI GKGCMC 1 cut(s) 150
BstV1I GCAGC 1 cut(s) 292
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 1 cut(s) 140
BstYI RGATCY 1 cut(s) 140
BstZI CGGCCG 1 cut(s) 14
BsuRI GGCC 1 cut(s) 16
BtgI CCRYGG 1 cut(s) 66
BtgZI GCGATG 1 cut(s) 504
BtsCI GGATG 3 cut(s) 337, 476, 883
BtsI GCAGTG 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 81
Cac8I GCNNGC 2 cut(s) 285, 682
CciNI GCGGCCGC 1 cut(s) 14
CfoI GCGC 1 cut(s) 729
Cfr10I RCCGGY 1 cut(s) 680
CviAII CATG 1 cut(s) 67
DdeI CTNAG 3 cut(s) 47, 154, 822
DpnI GATC 3 cut(s) 142, 367, 770
DpnII GATC 3 cut(s) 140, 365, 768
DraI TTTAAA 2 cut(s) 406, 747
EaeI YGGCCR 1 cut(s) 14
EagI CGGCCG 1 cut(s) 14
Eam1104I CTCTTC 4 cut(s) 90, 153, 584, 730
EarI CTCTTC 4 cut(s) 90, 153, 584, 730
EclXI CGGCCG 1 cut(s) 14
Eco130I CCWWGG 2 cut(s) 66, 288
Eco52I CGGCCG 1 cut(s) 14
EcoRI GAATTC 2 cut(s) 259, 596
EcoT14I CCWWGG 2 cut(s) 66, 288
ErhI CCWWGG 2 cut(s) 66, 288
Esp3I CGTCTC 1 cut(s) 430
FaeI CATG 1 cut(s) 70
FaiI YATR 8 cut(s) 39, 68, 104, 486, 647, 759, 776, 786
FaqI GGGAC 1 cut(s) 642
FatI CATG 1 cut(s) 66
FbaI TGATCA 1 cut(s) 768
FblI GTMKAC 1 cut(s) 550
Fnu4HI GCNGC 7 cut(s) 14, 17, 20, 23, 26, 29, 281
FokI GGATG 3 cut(s) 324, 483, 890
Fsp4HI GCNGC 7 cut(s) 14, 17, 20, 23, 26, 29, 281
GlaI GCGC 1 cut(s) 728
GluI GCNGC 7 cut(s) 14, 17, 20, 23, 26, 29, 281
GsaI CCCAGC 1 cut(s) 798
GsuI CTGGAG 1 cut(s) 152
HaeIII GGCC 1 cut(s) 16
HapII CCGG 5 cut(s) 54, 660, 681, 732, 852
HhaI GCGC 1 cut(s) 729
Hin1II CATG 1 cut(s) 70
Hin6I GCGC 1 cut(s) 727
HinP1I GCGC 1 cut(s) 727
HindIII AAGCTT 3 cut(s) 224, 378, 824
HinfI GANTC 2 cut(s) 218, 655
HpaII CCGG 5 cut(s) 54, 660, 681, 732, 852
HphI GGTGA 2 cut(s) 778, 781
Hpy166II GTNNAC 2 cut(s) 347, 551
Hpy188I TCNGA 3 cut(s) 157, 258, 901
Hpy188III TCNNGA 4 cut(s) 54, 116, 601, 732
Hpy8I GTNNAC 2 cut(s) 347, 551
HpyAV CCTTC 3 cut(s) 157, 707, 828
HpyCH4IV ACGT 2 cut(s) 185, 547
HpyCH4V TGCA 4 cut(s) 204, 303, 337, 867
HpyF10VI GCNNNNNNNGC 4 cut(s) 19, 22, 25, 28
HpyF3I CTNAG 3 cut(s) 47, 154, 822
HpySE526I ACGT 2 cut(s) 185, 547
Hsp92II CATG 1 cut(s) 70
HspAI GCGC 1 cut(s) 727
Kpn2I TCCGGA 2 cut(s) 53, 731
KroI GCCGGC 1 cut(s) 680
KroNI GCCGGC 1 cut(s) 682
Ksp22I TGATCA 1 cut(s) 768
Kzo9I GATC 3 cut(s) 140, 365, 768
LguI GCTCTTC 1 cut(s) 730
LmnI GCTCC 4 cut(s) 277, 578, 734, 854
Lsp1109I GCAGC 1 cut(s) 292
LweI GCATC 2 cut(s) 346, 667
MaeII ACGT 2 cut(s) 185, 547
MaeIII GTNAC 3 cut(s) 59, 590, 787
MalI GATC 3 cut(s) 142, 367, 770
MboI GATC 3 cut(s) 140, 365, 768
MboII GAAGA 8 cut(s) 107, 170, 193, 227, 571, 686, 747, 793
MfeI CAATTG 3 cut(s) 210, 620, 844
MflI RGATCY 1 cut(s) 140
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 8 cut(s) 111, 210, 250, 259, 536, 596, 620, 844
MlyI GAGTC 1 cut(s) 664
MnlI CCTC 6 cut(s) 50, 56, 91, 580, 587, 757
MroI TCCGGA 2 cut(s) 53, 731
MroNI GCCGGC 1 cut(s) 680
MseI TTAA 5 cut(s) 387, 405, 447, 539, 746
MslI CAYNNNNRTG 2 cut(s) 342, 489
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 5 cut(s) 54, 660, 681, 732, 852
MspR9I CCNGG 1 cut(s) 660
MunI CAATTG 3 cut(s) 210, 620, 844
Mva1269I GAATGC 1 cut(s) 305
MwoI GCNNNNNNNGC 4 cut(s) 19, 22, 25, 28
NaeI GCCGGC 1 cut(s) 682
NciI CCSGG 1 cut(s) 660
NcoI CCATGG 1 cut(s) 66
NdeII GATC 3 cut(s) 140, 365, 768
NgoMIV GCCGGC 1 cut(s) 680
NlaIII CATG 1 cut(s) 70
NmuCI GTSAC 1 cut(s) 787
NotI GCGGCCGC 1 cut(s) 14
PciSI GCTCTTC 1 cut(s) 730
PctI GAATGC 1 cut(s) 305
PdiI GCCGGC 1 cut(s) 682
PfeI GAWTC 1 cut(s) 218
PfoI TCCNGGA 1 cut(s) 658
PkrI GCNGC 7 cut(s) 15, 18, 21, 24, 27, 30, 282
PleI GAGTC 1 cut(s) 663
PpsI GAGTC 1 cut(s) 663
PshBI ATTAAT 1 cut(s) 447
PspFI CCCAGC 1 cut(s) 794
PsuI RGATCY 1 cut(s) 140
RseI CAYNNNNRTG 2 cut(s) 342, 489
SapI GCTCTTC 1 cut(s) 730
SaqAI TTAA 5 cut(s) 387, 405, 447, 539, 746
SatI GCNGC 7 cut(s) 14, 17, 20, 23, 26, 29, 281
Sau3AI GATC 3 cut(s) 140, 365, 768
SchI GAGTC 1 cut(s) 664
ScrFI CCNGG 1 cut(s) 660
SduI GDGCHC 1 cut(s) 150
SfaNI GCATC 2 cut(s) 346, 667
SmiMI CAYNNNNRTG 2 cut(s) 342, 489
SmlI CTYRAG 1 cut(s) 633
SmoI CTYRAG 1 cut(s) 633
Sse9I AATT 8 cut(s) 111, 210, 250, 259, 536, 596, 620, 844
SsiI CCGC 7 cut(s) 13, 17, 20, 23, 26, 29, 374
SspI AATATT 2 cut(s) 174, 863
StyD4I CCNGG 1 cut(s) 658
StyI CCWWGG 2 cut(s) 66, 288
TaiI ACGT 2 cut(s) 188, 550
TaqI TCGA 4 cut(s) 115, 221, 263, 327
TasI AATT 8 cut(s) 111, 210, 250, 259, 536, 596, 620, 844
TauI GCSGC 6 cut(s) 16, 19, 22, 25, 28, 31
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 5 cut(s) 387, 405, 447, 539, 746
Tru9I TTAA 5 cut(s) 387, 405, 447, 539, 746
TscAI CASTG 1 cut(s) 88
TseFI GTSAC 1 cut(s) 787
TseI GCWGC 1 cut(s) 280
Tsp45I GTSAC 1 cut(s) 787
TspDTI ATGAA 5 cut(s) 17, 138, 507, 558, 906
TspRI CASTG 1 cut(s) 88
VspI ATTAAT 1 cut(s) 447
XapI RAATTY 2 cut(s) 259, 596
XmiI GTMKAC 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.