Rh7AG444800

ADP binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000546
Physical Location & Seq
Forward (+)
60370169 .. 60375737
5569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG444800.1

Sequence Viewer

Length: 3027 bp
ATGGAATGGCCCGAGAGTTCGAAACCCGAGGAAGTGAAGCACCCAAAGGAGGTCGAGGAAGAGGAGAAATTTAAGGTTGGTCTGCAGTCAGGACGGCTTAAGCCGCCGCCGCGGATGAGTGTTCCAGTGATTGACAATGCATCCTCGACATGGGACATTATGAGGGGCTTAGGGCCCGAAGGTCAAACTTCTCATGAAGAAGTAGAAGAAGAGGATAATTTGATTACTTCTGATGATGAAGAAGATCATATTTCGATTAAAGAGATTGTATCGAGCTCCGGGTATTCTTCTACTACTTGGAATGACGATGACTCTTCTAGCACTACCACAGACCCCTCCACTATTTCTTCTTGGGAAAAGGAAACCTCTCGCGGGTATTTACCCCTGTATGAAGGAATTTCCGATAAGGTAACTAAAACGATGGATCCCCTGAAGACCCGACAGAAGCCAAAGTTCGTTAGGTGCAATGCGCTGAAGCACATTGACTATGATGCTGTTACATCTTCTTCCTCCTCGCTCTGCCACCACTCTGCGGGGTCACCACTCTACTCAGGCTCGGTCAATATTTCAGACCGGACGAGCTTCCAAGTAGAAGAAAACGACGGTGAGTTCGAACAGATATGCAATGAGTTGGGATTCTCCTGCCCTGAGTCCTTGGAGTTCCACTCGACGAACTCGGAATTGAAGCTAAAGGAGAACTGCAATCGCAAAGAAATGGAAGAAGTAGAAATTGATAAAAGAGCTGATGAAGAAACTAATTTGAGAGCAGAAGAAAACTTCAAATTACCAGGAGTATTACATAATAGCGCTTCTTCTTTTATGCATCCAGAACTGTTGGCAATTGAGCCCGACTCGTTCATTCCATTACAAACCTCTGGTGTCTCTCTGCGCTTGATCGGACAACTTCCTCGTAATTTTTTGTTGAACTTCAAGTTCTTGACTGCCTTGCATCTTAGTCGAAAGCTGGATTTTGATTTGCCAAGTTCAATTGGGGATGTAAAATCCTTACAATGTCTAGATGTCTCATATACACCAATCTCATGGTTGCCCGAATCCATAGGTTTACTTCAAAAGTTACAGACCCTAAAACTCAGAAGTTGTAGAAACCTCGTTCAGTTGCCTGAATCAATTACTTCTCTTGATGAGTTACAGACCTTACATCTCAGAGGTTGTGTCAACCTTATTGCTTTACCTAAGGGAATAAAGGTACTAATAAAGCTGCGTGATCTTGATGTTATTAACTGTCCGAAATTGAAACTTTCAGGGGAGCCAAATGAGAAGCTGATTTCTTCAGAAATAAATTTGTCAGAGTCATTGGAATTGAAGGAAAGCTATGCAAATTTGCAAGAAAAGGTTGTGCATGAAGATGAAGAATATGACAGTTTACCCAAACAATTAAAAAGGTTATGTCTGTATTGTTCTCTCTTTCCATCACATTATGAGTTTAGAAGAGATGAGTTGGTTCAAATGTGGATAGCTGAAGGCCTTATTGAAGAGATCCACGGAGGGGTAATGGAGGACATTGGTGGTGAGTATTTCATGTCTTTGCAGAACAAAAGTTTTCTTGTGCCTTCAAGATGTGACTACAGATTGGATTTTGATTCGGTATTTTCTGTGCCCATTTATAATCCTGGCCACTTATTGTATAAAGGAAATCCACTCAAGCTTTCACGTTTGGGAAGGACAATTGCTCCGTTTGAGTATTTCGTAGCTGTGGATGGTAGGTTGGATGGTGCCTTGGAAATGACACAGCATTTGTCTTTAATTTGCAAAGACATTGATGAGATGACTTTTGGGGTTCTTAAAAAATTTAAGCATTTGCGTACACTTCTTGTGCTTGCTGGTTGTGGATCTTCCATCAAATATGTTCCTCGTGATCTGTTTTTGAGCTTCAGGGAATTGAGAACACTGAATTTGAGTCAAACACTCGTCTCTGAATTGCCAAGTTCCATTCGAAATGCAAAGTCATTACGATACCTTGATCTCTCCAATACTCTTATTACAAGGTTGCCGGAATCAATAGATTCTCTTCACACTTTACAGACAGTAAGACTCAGAGGTTGTGTACACTTTCTTCAATTACCAAAGGGGATGAAGAAGCTAATCAATCTACGACATATCGATTTGGATATCATTCGACAGTTAAGGTCCATGCCTCCCCATTTGGGAAGCTTAACCAACCTTCAGACTTTGTCAGCATTTCTTGTTGGTCGAGATGCTGGGTGCCGTGTAGGAGAGCTTAAGAATTTGAATGATCTCAGAGGAGTACTTCGCATTTCAAGGCTTGAAAATGTGTCATGCCAGGAAGAGGCTAAGGCAGCTGCTTTGATCGAAAAGATATACCTGCAAAGGTTGGAGCTCCGCTGGAGTAGTGTGTTCGCCGAGAATACGGAAGAGCAAGAGAAAATTCTTGAATGTCTTCAACCTCATGCTGGCCTTAAAGAGCTACAAATACAACAATATGCCGGGTCAAGACTTCCAACTTGGATTAGCAATCCATGTTTTACTGAACTTATTGTTCTTACTCTCTACAGATGCAAACACTGTGAGCTTCTCCCATCTATTGGGCAGTTACCAATGCTAAAGTCTCTTTCTATTATAGAAATGAATGAGGTGAAAGAAATCAACTACCAGTTTTTGGGAAAGAAAAACCCAGTTGATCAAGGGTTTCATGCATTTTCGAAACTGGAGATACTAGAAGTTGACATCATGCTCAATTTGAAACTGTGGAAGGATGTAAACATTGGTGACTTACCTTCCTTACTTAAACTCACAGTTGATTCTTGCCCTGAACTTACCACTCTTCCATCACTGTCTTGGCTCAAATCCCTCAAGCATTTGGAACTCAGGCGCTGTCCAAAAATTCTGACCTGGCCTTGTGATGGACTACCAGCATCACTTGAATCTTTCATAGTAATAGATTGCCCGGAACTGAAAGAATGGTGCCGCGTGATGGAAGGTGAAGATTGGAGGAAGAAATGCCATCTCCCTAGCATATGGCTTGATTGTGAAGAGTTGTGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 ko04016 ko04075 ko04931 map04014 map04016 map04075 map04931
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1008

Amino Acids

114.69

Weight (kDa)

5.44

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 310 - 437 8e-14 Leucine-rich repeat region
WHD_DRP PF23559 475 - 523 3.6e-14 Disease resistance protein Winged helix domain
LRR_14 PF23598 627 - 927 8.7e-26 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 745 - 869 9.1e-39 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 830 - 948 3.2e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000546)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700
malus_domestica MD14G1049300.v1.1
rosa_chinensis RchiOBHm_Chr4g0433201 RchiOBHm_Chr6g0255111 RchiOBHm_Chr7g0235301 RchiOBHm_Chr7g0235351 RchiOBHm_Chr7g0235361 RchiOBHm_Chr7g0235381
rosa_laevigata RLG00000001164 RLG00000013941
rosa_multiflora Rmu_co8331033.1_g000001 Rmu_co8369635.1_g000001 Rmu_sc0000957.1_g000010 Rmu_sc0003304.1_g000033 Rmu_sc0003304.1_g000041 Rmu_sc0003488.1_g000001 Rmu_sc0004529.1_g000036 Rmu_sc0004637.1_g000013 Rmu_sc0004657.1_g000070 Rmu_sc0004657.1_g000076 Rmu_sc0006578.1_g000004 Rmu_sc0011027.1_g000008 Rmu_sc0011027.1_g000010 Rmu_sc0013813.1_g000001 Rmu_sc0014112.1_g000001 Rmu_sc0014112.1_g000002 Rmu_sc0014112.1_g000004 Rmu_ssc0000011.1_g000040 Rmu_ssc0000295.1_g000011
rosa_roxburghii Rroxscaffold_2G00103040 Rroxscaffold_3G00226100 Rroxscaffold_3G00226120 Rroxscaffold_5G00374710 Rroxscaffold_5G00374720 Rroxscaffold_7G00200160 Rroxscaffold_7G00200190
rosa_rugosa Rorug05G0474400 Rorug05G0474400 Rorug06G0037400 Rorug06G0037500 Rorug06G0037600 Rorug06G0038300 Rorug06G0038300 Rorug06G0038400 Rorug06G0038500 Rorug07G0289800 Rorug07G0289900 Rorug07G0290000 Rorug07G0290000
rosa_samantha Rh4AG320500 Rh4AG320700 Rh4AG321100 Rh4DG324200 Rh4DG324700 Rh6BG067500 Rh6CG066600 Rh6CG066700 Rh6DG063600 Rh6DG063700 Rh6DG147600 Rh7AG444800 Rh7AG445000 Rh7BG416700 Rh7BG416800 Rh7CG465200 Rh7CG465300 Rh7CG465700 Rh7DG433600 Rh7DG433800
rosa_wichuraiana Rw0G008730 Rw0G008770 Rw4G027830 Rw7G036940 Rw7G036980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1626
Acc36I ACCTGC 1 cut(s) 2352
AccB1I GGYRCC 3 cut(s) 1733, 2223, 2943
AccB7I CCANNNNNTGG 2 cut(s) 2564, 2638
AccII CGCG 3 cut(s) 112, 372, 2949
AciI CCGC 8 cut(s) 104, 107, 110, 112, 372, 533, 2362, 2947
AclWI GGATC 4 cut(s) 419, 432, 1492, 1858
AcoI YGGCCR 1 cut(s) 1633
AcsI RAATTY 9 cut(s) 68, 396, 1300, 1339, 1808, 1912, 2245, 2406, 2862
AcuI CTGAAG 6 cut(s) 452, 494, 1275, 1500, 1876, 2168
AfaI GTAC 4 cut(s) 1209, 1825, 2067, 2268
AfeI AGCGCT 1 cut(s) 808
AflII CTTAAG 2 cut(s) 98, 2240
AjnI CCWGG 4 cut(s) 787, 1630, 2301, 2870
Alw21I GWGCWC 2 cut(s) 278, 2361
Alw26I GTCTC 4 cut(s) 886, 1027, 1936, 2592
AlwI GGATC 4 cut(s) 419, 432, 1492, 1858
AlwNI CAGNNNCTG 1 cut(s) 2853
Ama87I CYCGRG 2 cut(s) 11, 26
Aor51HI AGCGCT 1 cut(s) 808
AoxI GGCC 6 cut(s) 8, 173, 1483, 1633, 2434, 2873
ApaI GGGCCC 1 cut(s) 177
ApeKI GCWGC 3 cut(s) 1219, 2318, 2321
ApoI RAATTY 9 cut(s) 68, 396, 1300, 1339, 1808, 1912, 2245, 2406, 2862
ArsI GACNNNNNNTTYG 4 cut(s) 593, 625, 1738, 1770
Asp700I GAANNNNTTC 1 cut(s) 2418
AspLEI GCGC 4 cut(s) 472, 809, 891, 2853
AspS9I GGNCC 4 cut(s) 9, 173, 174, 2148
AsuC2I CCSGG 3 cut(s) 280, 2467, 2927
AsuHPI GGTGA 6 cut(s) 531, 617, 1541, 2626, 2759, 2972
AsuII TTCGAA 4 cut(s) 20, 612, 1954, 2681
AvaI CYCGRG 2 cut(s) 11, 26
AvaII GGWCC 1 cut(s) 2148
AxyI CCTNAGG 1 cut(s) 1194
BaeGI GKGCMC 2 cut(s) 177, 1620
BalI TGGCCA 1 cut(s) 1635
BamHI GGATCC 1 cut(s) 424
BanI GGYRCC 3 cut(s) 1733, 2223, 2943
BanII GRGCYC 4 cut(s) 177, 278, 849, 2361
BarI GAAGNNNNNNTAC 2 cut(s) 2058, 2090
BauI CACGAG 1 cut(s) 1872
BbsI GAAGAC 2 cut(s) 440, 2411
Bbv12I GWGCWC 2 cut(s) 278, 2361
BbvI GCAGC 3 cut(s) 1206, 2308, 2330
BceAI ACGGC 2 cut(s) 110, 2211
BcgI CGANNNNNNTGC 2 cut(s) 938, 972
BciT130I CCWGG 4 cut(s) 789, 1632, 2303, 2872
BclI TGATCA 1 cut(s) 2659
BcnI CCSGG 3 cut(s) 280, 2467, 2927
BcoDI GTCTC 4 cut(s) 886, 1027, 1936, 2592
BfaI CTAG 4 cut(s) 318, 1016, 2696, 2991
BfmI CTRYAG 3 cut(s) 83, 1585, 2530
BfoI RGCGCY 2 cut(s) 810, 2854
BfrI CTTAAG 2 cut(s) 98, 2240
BfuAI ACCTGC 1 cut(s) 2352
BisI GCNGC 7 cut(s) 104, 107, 110, 1220, 2319, 2322, 2947
BlsI GCNGC 7 cut(s) 105, 108, 111, 1221, 2320, 2323, 2948
BmcAI AGTACT 1 cut(s) 2268
Bme1390I CCNGG 7 cut(s) 280, 789, 1632, 2303, 2467, 2872, 2927
Bme18I GGWCC 1 cut(s) 2148
BmeT110I CYCGRG 2 cut(s) 11, 26
BmgT120I GGNCC 4 cut(s) 9, 173, 174, 2148
BmiI GGNNCC 6 cut(s) 175, 426, 1269, 1735, 2225, 2945
BmrFI CCNGG 7 cut(s) 280, 789, 1632, 2303, 2467, 2872, 2927
BmrI ACTGGG 1 cut(s) 2648
BmsI GCATC 7 cut(s) 149, 481, 832, 958, 2206, 2525, 2903
BmuI ACTGGG 1 cut(s) 2648
BpiI GAAGAC 2 cut(s) 440, 2411
BplI GAGNNNNNCTC 4 cut(s) 650, 682, 836, 868
BpmI CTGGAG 2 cut(s) 2386, 2708
Bpu10I CCTNAGC 2 cut(s) 169, 2313
Bpu14I TTCGAA 4 cut(s) 20, 612, 1954, 2681
BpuEI CTTGAG 2 cut(s) 1646, 2816
BpuMI CCSGG 3 cut(s) 280, 2467, 2927
Bsa29I ATCGAT 1 cut(s) 2121
BsaJI CCNNGG 5 cut(s) 27, 110, 654, 1501, 1737
BsaWI WCCGGW 1 cut(s) 573
BsaXI ACNNNNNCTCC 2 cut(s) 1675, 1705
Bse1I ACTGG 4 cut(s) 125, 2632, 2654, 2691
Bse21I CCTNAGG 1 cut(s) 1194
Bse3DI GCAATG 2 cut(s) 472, 631
BseBI CCWGG 4 cut(s) 789, 1632, 2303, 2872
BseCI ATCGAT 1 cut(s) 2121
BseDI CCNNGG 5 cut(s) 27, 110, 654, 1501, 1737
BseGI GGATG 8 cut(s) 120, 140, 823, 1000, 1723, 1735, 2097, 2740
BseMI GCAATG 2 cut(s) 472, 631
BseMII CTCAG 7 cut(s) 564, 639, 1105, 1177, 2068, 2272, 2860
BseNI ACTGG 4 cut(s) 125, 2632, 2654, 2691
BseRI GAGGAG 3 cut(s) 77, 502, 2277
BseSI GKGCMC 2 cut(s) 177, 1620
BseXI GCAGC 3 cut(s) 1206, 2308, 2330
BseYI CCCAGC 1 cut(s) 2219
Bsh1236I CGCG 3 cut(s) 112, 372, 2949
BshFI GGCC 6 cut(s) 10, 175, 1485, 1635, 2436, 2875
BshNI GGYRCC 3 cut(s) 1733, 2223, 2943
BshVI ATCGAT 1 cut(s) 2121
BsiHKAI GWGCWC 2 cut(s) 278, 2361
BsiHKCI CYCGRG 2 cut(s) 11, 26
BsiSI CCGG 5 cut(s) 279, 574, 2012, 2466, 2927
BslFI GGGAC 1 cut(s) 167
BsmAI GTCTC 4 cut(s) 886, 1027, 1936, 2592
BsmBI CGTCTC 1 cut(s) 1936
BsmFI GGGAC 1 cut(s) 167
BsnI GGCC 6 cut(s) 10, 175, 1485, 1635, 2436, 2875
BsoBI CYCGRG 2 cut(s) 11, 26
Bsp119I TTCGAA 4 cut(s) 20, 612, 1954, 2681
Bsp120I GGGCCC 1 cut(s) 173
Bsp1286I GDGCHC 5 cut(s) 177, 278, 849, 1620, 2361
Bsp1407I TGTACA 1 cut(s) 2065
BspACI CCGC 8 cut(s) 104, 107, 110, 112, 372, 533, 2362, 2947
BspANI GGCC 6 cut(s) 10, 175, 1485, 1635, 2436, 2875
BspCNI CTCAG 7 cut(s) 563, 640, 1104, 1176, 2067, 2271, 2859
BspDI ATCGAT 1 cut(s) 2121
BspFNI CGCG 3 cut(s) 112, 372, 2949
BspHI TCATGA 1 cut(s) 193
BspLI GGNNCC 6 cut(s) 175, 426, 1269, 1735, 2225, 2945
BspMAI CTGCAG 1 cut(s) 87
BspMI ACCTGC 1 cut(s) 2352
BspPI GGATC 4 cut(s) 419, 432, 1492, 1858
BspQI GCTCTTC 1 cut(s) 2388
BspT104I TTCGAA 4 cut(s) 20, 612, 1954, 2681
BspT107I GGYRCC 3 cut(s) 1733, 2223, 2943
BspTI CTTAAG 2 cut(s) 98, 2240
BsrDI GCAATG 2 cut(s) 472, 631
BsrGI TGTACA 1 cut(s) 2065
BsrI ACTGG 4 cut(s) 125, 2632, 2654, 2691
BssECI CCNNGG 5 cut(s) 27, 110, 654, 1501, 1737
BssSI CACGAG 1 cut(s) 1872
BssT1I CCWWGG 2 cut(s) 654, 1737
Bst2BI CACGAG 1 cut(s) 1872
Bst2UI CCWGG 4 cut(s) 789, 1632, 2303, 2872
BstAFI CTTAAG 2 cut(s) 98, 2240
BstAUI TGTACA 1 cut(s) 2065
BstBI TTCGAA 4 cut(s) 20, 612, 1954, 2681
BstC8I GCNNGC 2 cut(s) 1839, 2434
BstDSI CCRYGG 2 cut(s) 110, 1501
BstEII GGTNACC 1 cut(s) 537
BstF5I GGATG 8 cut(s) 120, 140, 823, 1000, 1723, 1735, 2097, 2740
BstFNI CGCG 3 cut(s) 112, 372, 2949
BstH2I RGCGCY 2 cut(s) 810, 2854
BstHHI GCGC 4 cut(s) 472, 809, 891, 2853
BstMAI GTCTC 4 cut(s) 886, 1027, 1936, 2592
BstMWI GCNNNNNNNGC 3 cut(s) 103, 109, 2318
BstNI CCWGG 4 cut(s) 789, 1632, 2303, 2872
BstPI GGTNACC 1 cut(s) 537
BstSCI CCNGG 7 cut(s) 278, 787, 1630, 2301, 2465, 2870, 2925
BstSFI CTRYAG 3 cut(s) 83, 1585, 2530
BstSLI GKGCMC 2 cut(s) 177, 1620
BstUI CGCG 3 cut(s) 112, 372, 2949
BstV1I GCAGC 3 cut(s) 1206, 2308, 2330
BstV2I GAAGAC 2 cut(s) 440, 2411
BstX2I RGATCY 3 cut(s) 424, 1497, 1850
BstXI CCANNNNNNTGG 1 cut(s) 1041
BstYI RGATCY 3 cut(s) 424, 1497, 1850
Bsu15I ATCGAT 1 cut(s) 2121
Bsu36I CCTNAGG 1 cut(s) 1194
BsuRI GGCC 6 cut(s) 10, 175, 1485, 1635, 2436, 2875
BsuTUI ATCGAT 1 cut(s) 2121
BtgI CCRYGG 2 cut(s) 110, 1501
BtsCI GGATG 8 cut(s) 120, 140, 823, 1000, 1723, 1735, 2097, 2740
BtsIMutI CAGTG 4 cut(s) 132, 1907, 2542, 2810
BveI ACCTGC 1 cut(s) 2352
Cac8I GCNNGC 2 cut(s) 1839, 2434
CaiI CAGNNNCTG 1 cut(s) 2853
CciI TCATGA 1 cut(s) 193
CfoI GCGC 4 cut(s) 472, 809, 891, 2853
Cfr13I GGNCC 4 cut(s) 9, 173, 174, 2148
Cfr42I CCGCGG 1 cut(s) 113
ClaI ATCGAT 1 cut(s) 2121
Csp6I GTAC 4 cut(s) 1208, 1824, 2066, 2267
CviQI GTAC 4 cut(s) 1208, 1824, 2066, 2267
EaeI YGGCCR 1 cut(s) 1633
Ecl136II GAGCTC 2 cut(s) 276, 2359
Eco130I CCWWGG 2 cut(s) 654, 1737
Eco147I AGGCCT 1 cut(s) 1485
Eco24I GRGCYC 4 cut(s) 177, 278, 849, 2361
Eco32I GATATC 1 cut(s) 2131
Eco47I GGWCC 1 cut(s) 2148
Eco47III AGCGCT 1 cut(s) 808
Eco53kI GAGCTC 2 cut(s) 276, 2359
Eco57I CTGAAG 6 cut(s) 452, 494, 1275, 1500, 1876, 2168
Eco81I CCTNAGG 1 cut(s) 1194
Eco88I CYCGRG 2 cut(s) 11, 26
Eco91I GGTNACC 1 cut(s) 537
EcoICRI GAGCTC 2 cut(s) 276, 2359
EcoO109I RGGNCCY 1 cut(s) 173
EcoO65I GGTNACC 1 cut(s) 537
EcoRII CCWGG 4 cut(s) 787, 1630, 2301, 2870
EcoRV GATATC 1 cut(s) 2131
EcoT14I CCWWGG 2 cut(s) 654, 1737
EcoT22I ATGCAT 3 cut(s) 142, 825, 2677
EcoT38I GRGCYC 4 cut(s) 177, 278, 849, 2361
ErhI CCWWGG 2 cut(s) 654, 1737
Esp3I CGTCTC 1 cut(s) 1936
FaqI GGGAC 1 cut(s) 167
FauI CCCGC 2 cut(s) 365, 526
FauNDI CATATG 1 cut(s) 2996
FbaI TGATCA 1 cut(s) 2659
Fnu4HI GCNGC 7 cut(s) 104, 107, 110, 1220, 2319, 2322, 2947
FokI GGATG 8 cut(s) 127, 127, 810, 1007, 1730, 1742, 2104, 2747
FriOI GRGCYC 4 cut(s) 177, 278, 849, 2361
Fsp4HI GCNGC 7 cut(s) 104, 107, 110, 1220, 2319, 2322, 2947
FspBI CTAG 4 cut(s) 318, 1016, 2696, 2991
GlaI GCGC 4 cut(s) 471, 808, 890, 2852
GluI GCNGC 7 cut(s) 104, 107, 110, 1220, 2319, 2322, 2947
GsaI CCCAGC 1 cut(s) 2223
GsuI CTGGAG 2 cut(s) 2386, 2708
HaeII RGCGCY 2 cut(s) 810, 2854
HaeIII GGCC 6 cut(s) 10, 175, 1485, 1635, 2436, 2875
HapII CCGG 5 cut(s) 279, 574, 2012, 2466, 2927
HhaI GCGC 4 cut(s) 472, 809, 891, 2853
Hin6I GCGC 4 cut(s) 470, 807, 889, 2851
HinP1I GCGC 4 cut(s) 470, 807, 889, 2851
HincII GTYRAC 2 cut(s) 1177, 2704
HindII GTYRAC 2 cut(s) 1177, 2704
HindIII AAGCTT 2 cut(s) 1664, 2170
HpaII CCGG 5 cut(s) 279, 574, 2012, 2466, 2927
HphI GGTGA 6 cut(s) 531, 617, 1541, 2626, 2759, 2972
Hpy166II GTNNAC 9 cut(s) 1064, 1177, 1385, 1826, 2066, 2068, 2704, 2740, 3021
Hpy8I GTNNAC 9 cut(s) 1064, 1177, 1385, 1826, 2066, 2068, 2704, 2740, 3021
Hpy99I CGWCG 2 cut(s) 605, 673
HpyCH4IV ACGT 1 cut(s) 1672
HpyF10VI GCNNNNNNNGC 3 cut(s) 103, 109, 2318
HpySE526I ACGT 1 cut(s) 1672
HspAI GCGC 4 cut(s) 470, 807, 889, 2851
Ksp22I TGATCA 1 cut(s) 2659
KspI CCGCGG 1 cut(s) 113
LguI GCTCTTC 1 cut(s) 2388
LmnI GCTCC 5 cut(s) 281, 1267, 1696, 2356, 2364
Lsp1109I GCAGC 3 cut(s) 1206, 2308, 2330
LweI GCATC 7 cut(s) 149, 481, 832, 958, 2206, 2525, 2903
MaeI CTAG 4 cut(s) 318, 1016, 2696, 2991
MaeII ACGT 1 cut(s) 1672
MaeIII GTNAC 8 cut(s) 409, 496, 537, 1074, 1146, 1580, 2571, 2747
MfeI CAATTG 3 cut(s) 840, 987, 1686
MflI RGATCY 3 cut(s) 424, 1497, 1850
MhlI GDGCHC 5 cut(s) 177, 278, 849, 1620, 2361
MlsI TGGCCA 1 cut(s) 1635
MluNI TGGCCA 1 cut(s) 1635
MlyI GAGTC 6 cut(s) 305, 659, 845, 1319, 1927, 2046
MmeI TCCRAC 3 cut(s) 1707, 2334, 2504
Mox20I TGGCCA 1 cut(s) 1635
Mph1103I ATGCAT 3 cut(s) 142, 825, 2677
MroXI GAANNNNTTC 1 cut(s) 2418
MscI TGGCCA 1 cut(s) 1635
MslI CAYNNNNRTG 1 cut(s) 1365
Msp20I TGGCCA 1 cut(s) 1635
MspA1I CMGCKG 3 cut(s) 112, 2321, 2364
MspCI CTTAAG 2 cut(s) 98, 2240
MspI CCGG 5 cut(s) 279, 574, 2012, 2466, 2927
MspR9I CCNGG 7 cut(s) 280, 789, 1632, 2303, 2467, 2872, 2927
MunI CAATTG 3 cut(s) 840, 987, 1686
MvaI CCWGG 4 cut(s) 789, 1632, 2303, 2872
MvnI CGCG 3 cut(s) 112, 372, 2949
MwoI GCNNNNNNNGC 3 cut(s) 103, 109, 2318
NciI CCSGG 3 cut(s) 280, 2467, 2927
NdeI CATATG 1 cut(s) 2996
NlaIV GGNNCC 6 cut(s) 175, 426, 1269, 1735, 2225, 2945
NmeAIII GCCGAG 1 cut(s) 2407
NmuCI GTSAC 3 cut(s) 537, 1580, 2747
NsiI ATGCAT 3 cut(s) 142, 825, 2677
NspV TTCGAA 4 cut(s) 20, 612, 1954, 2681
PagI TCATGA 1 cut(s) 193
PceI AGGCCT 1 cut(s) 1485
PciSI GCTCTTC 1 cut(s) 2388
PcsI WCGNNNNNNNCGW 1 cut(s) 609
PdmI GAANNNNTTC 1 cut(s) 2418
PfeI GAWTC 8 cut(s) 636, 1052, 1124, 1601, 2015, 2024, 2780, 2903
PflFI GACNNNGTC 1 cut(s) 2191
PflMI CCANNNNNTGG 2 cut(s) 2564, 2638
PkrI GCNGC 7 cut(s) 105, 108, 111, 1221, 2320, 2323, 2948
PleI GAGTC 6 cut(s) 305, 658, 845, 1318, 1926, 2046
PpsI GAGTC 6 cut(s) 305, 658, 845, 1318, 1926, 2046
PsiI TTATAA 1 cut(s) 1626
Psp124BI GAGCTC 2 cut(s) 278, 2361
Psp6I CCWGG 4 cut(s) 787, 1630, 2301, 2870
PspEI GGTNACC 1 cut(s) 537
PspFI CCCAGC 1 cut(s) 2219
PspGI CCWGG 4 cut(s) 787, 1630, 2301, 2870
PspN4I GGNNCC 6 cut(s) 175, 426, 1269, 1735, 2225, 2945
PspOMI GGGCCC 1 cut(s) 173
PspPI GGNCC 4 cut(s) 9, 173, 174, 2148
PstI CTGCAG 1 cut(s) 87
PstNI CAGNNNCTG 1 cut(s) 2853
PsuI RGATCY 3 cut(s) 424, 1497, 1850
PsyI GACNNNGTC 1 cut(s) 2191
PvuII CAGCTG 1 cut(s) 2321
RsaI GTAC 4 cut(s) 1209, 1825, 2067, 2268
RsaNI GTAC 4 cut(s) 1208, 1824, 2066, 2267
RseI CAYNNNNRTG 1 cut(s) 1365
SacI GAGCTC 2 cut(s) 278, 2361
SacII CCGCGG 1 cut(s) 113
SapI GCTCTTC 1 cut(s) 2388
SatI GCNGC 7 cut(s) 104, 107, 110, 1220, 2319, 2322, 2947
Sau96I GGNCC 4 cut(s) 9, 173, 174, 2148
ScaI AGTACT 1 cut(s) 2268
SchI GAGTC 6 cut(s) 305, 659, 845, 1319, 1927, 2046
ScrFI CCNGG 7 cut(s) 280, 789, 1632, 2303, 2467, 2872, 2927
SduI GDGCHC 5 cut(s) 177, 278, 849, 1620, 2361
SfaNI GCATC 7 cut(s) 149, 481, 832, 958, 2206, 2525, 2903
SfcI CTRYAG 3 cut(s) 83, 1585, 2530
Sfr303I CCGCGG 1 cut(s) 113
SfuI TTCGAA 4 cut(s) 20, 612, 1954, 2681
SgrBI CCGCGG 1 cut(s) 113
SinI GGWCC 1 cut(s) 2148
SmiMI CAYNNNNRTG 1 cut(s) 1365
SmlI CTYRAG 4 cut(s) 98, 1661, 2240, 2831
SmoI CTYRAG 4 cut(s) 98, 1661, 2240, 2831
SseBI AGGCCT 1 cut(s) 1485
SsiI CCGC 8 cut(s) 104, 107, 110, 112, 372, 533, 2362, 2947
SspI AATATT 1 cut(s) 565
SspMI CTAG 4 cut(s) 318, 1016, 2696, 2991
SstI GAGCTC 2 cut(s) 278, 2361
StuI AGGCCT 1 cut(s) 1485
StyD4I CCNGG 7 cut(s) 278, 787, 1630, 2301, 2465, 2870, 2925
StyI CCWWGG 2 cut(s) 654, 1737
TaiI ACGT 1 cut(s) 1675
TaqII GACCGA 1 cut(s) 547
TatI WGTACW 2 cut(s) 2065, 2266
TauI GCSGC 4 cut(s) 106, 109, 112, 2949
TfiI GAWTC 8 cut(s) 636, 1052, 1124, 1601, 2015, 2024, 2780, 2903
TscAI CASTG 4 cut(s) 132, 1914, 2549, 2817
TseFI GTSAC 3 cut(s) 537, 1580, 2747
TseI GCWGC 3 cut(s) 1219, 2318, 2321
Tsp45I GTSAC 3 cut(s) 537, 1580, 2747
TspGWI ACGGA 3 cut(s) 1518, 1683, 2405
TspRI CASTG 4 cut(s) 132, 1914, 2549, 2817
Tth111I GACNNNGTC 1 cut(s) 2191
Van91I CCANNNNNTGG 2 cut(s) 2564, 2638
Vha464I CTTAAG 2 cut(s) 98, 2240
VpaK11BI GGWCC 1 cut(s) 2148
XapI RAATTY 9 cut(s) 68, 396, 1300, 1339, 1808, 1912, 2245, 2406, 2862
XbaI TCTAGA 1 cut(s) 1015
XcmI CCANNNNNNNNNTGG 1 cut(s) 2814
XmnI GAANNNNTTC 1 cut(s) 2418
XspI CTAG 4 cut(s) 318, 1016, 2696, 2991
ZrmI AGTACT 1 cut(s) 2268
Zsp2I ATGCAT 3 cut(s) 142, 825, 2677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.