ADP binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 469 |
| AccB1I | GGYRCC | 3 cut(s) | 469, 799, 1800 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1025, 1854 |
| AclWI | GGATC | 3 cut(s) | 100, 849, 1872 |
| AcoI | YGGCCR | 1 cut(s) | 154 |
| AcsI | RAATTY | 4 cut(s) | 667, 772, 1105, 1700 |
| AcuI | CTGAAG | 1 cut(s) | 1028 |
| AcyI | GRCGYC | 2 cut(s) | 41, 92 |
| AdeI | CACNNNGTG | 1 cut(s) | 898 |
| AfaI | GTAC | 4 cut(s) | 471, 1128, 1399, 1894 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 163, 188, 1025, 1292, 1424, 1739, 1810, 1854 |
| AflII | CTTAAG | 1 cut(s) | 1100 |
| AflIII | ACRYGT | 1 cut(s) | 724 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 130 |
| AjnI | CCWGG | 3 cut(s) | 135, 440, 1897 |
| AjuI | GAANNNNNNNTTGG | 3 cut(s) | 296, 328, 1916 |
| AluBI | AGCT | 7 cut(s) | 204, 750, 961, 1099, 1181, 1219, 1720 |
| AluI | AGCT | 7 cut(s) | 204, 750, 961, 1099, 1181, 1219, 1720 |
| Alw21I | GWGCWC | 3 cut(s) | 206, 928, 1221 |
| Alw26I | GTCTC | 2 cut(s) | 48, 1890 |
| Alw44I | GTGCAC | 1 cut(s) | 924 |
| AlwI | GGATC | 3 cut(s) | 100, 849, 1872 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1051 |
| AoxI | GGCC | 3 cut(s) | 154, 1008, 1294 |
| ApaLI | GTGCAC | 1 cut(s) | 924 |
| ApeKI | GCWGC | 5 cut(s) | 57, 82, 569, 1083, 1181 |
| ApoI | RAATTY | 4 cut(s) | 667, 772, 1105, 1700 |
| ArsI | GACNNNNNNTTYG | 8 cut(s) | 54, 86, 598, 630, 763, 795, 1709, 1741 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1278 |
| Asp718I | GGTACC | 1 cut(s) | 469 |
| AspLEI | GCGC | 1 cut(s) | 553 |
| AspS9I | GGNCC | 4 cut(s) | 132, 139, 166, 1008 |
| AsuC2I | CCSGG | 2 cut(s) | 186, 486 |
| AsuHPI | GGTGA | 4 cut(s) | 676, 884, 1616, 1829 |
| AsuII | TTCGAA | 1 cut(s) | 814 |
| AvaII | GGWCC | 3 cut(s) | 132, 139, 166 |
| BaeGI | GKGCMC | 3 cut(s) | 426, 928, 953 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 429, 462 |
| BanI | GGYRCC | 3 cut(s) | 469, 799, 1800 |
| BanII | GRGCYC | 2 cut(s) | 206, 1221 |
| BauI | CACGAG | 1 cut(s) | 732 |
| BbsI | GAAGAC | 3 cut(s) | 705, 1271, 1652 |
| Bbv12I | GWGCWC | 3 cut(s) | 206, 928, 1221 |
| BbvCI | CCTCAGC | 1 cut(s) | 1173 |
| BbvI | GCAGC | 5 cut(s) | 69, 94, 581, 1070, 1168 |
| BceAI | ACGGC | 2 cut(s) | 557, 1071 |
| BciT130I | CCWGG | 3 cut(s) | 137, 442, 1899 |
| BclI | TGATCA | 1 cut(s) | 1516 |
| BcnI | CCSGG | 2 cut(s) | 186, 486 |
| BcoDI | GTCTC | 2 cut(s) | 48, 1890 |
| BfaI | CTAG | 2 cut(s) | 429, 1214 |
| BfmI | CTRYAG | 1 cut(s) | 1390 |
| BfrI | CTTAAG | 1 cut(s) | 1100 |
| BisI | GCNGC | 9 cut(s) | 11, 14, 58, 83, 157, 570, 1084, 1182, 1804 |
| BlsI | GCNGC | 9 cut(s) | 12, 15, 59, 84, 158, 571, 1085, 1183, 1805 |
| BmcAI | AGTACT | 1 cut(s) | 1128 |
| Bme1390I | CCNGG | 5 cut(s) | 137, 186, 442, 486, 1899 |
| Bme18I | GGWCC | 3 cut(s) | 132, 139, 166 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 130 |
| BmgT120I | GGNCC | 4 cut(s) | 132, 139, 166, 1008 |
| BmiI | GGNNCC | 8 cut(s) | 133, 134, 168, 216, 471, 595, 801, 1802 |
| BmrFI | CCNGG | 5 cut(s) | 137, 186, 442, 486, 1899 |
| BpiI | GAAGAC | 3 cut(s) | 705, 1271, 1652 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1173 |
| Bpu14I | TTCGAA | 1 cut(s) | 814 |
| BpuEI | CTTGAG | 3 cut(s) | 1565, 1673, 1778 |
| BpuMI | CCSGG | 2 cut(s) | 186, 486 |
| BsaHI | GRCGYC | 2 cut(s) | 41, 92 |
| BsaJI | CCNNGG | 4 cut(s) | 135, 441, 573, 597 |
| BsaWI | WCCGGW | 1 cut(s) | 1221 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 163, 188, 1025, 1292, 1424, 1739, 1810, 1854 |
| Bse118I | RCCGGY | 2 cut(s) | 152, 553 |
| Bse1I | ACTGG | 2 cut(s) | 382, 1839 |
| Bse3DI | GCAATG | 1 cut(s) | 712 |
| BseBI | CCWGG | 3 cut(s) | 137, 442, 1899 |
| BseDI | CCNNGG | 4 cut(s) | 135, 441, 573, 597 |
| BseGI | GGATG | 5 cut(s) | 284, 333, 583, 1613, 1942 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 163, 188, 1025, 1292, 1424, 1739, 1810, 1854 |
| BseMI | GCAATG | 1 cut(s) | 712 |
| BseMII | CTCAG | 3 cut(s) | 543, 1164, 1233 |
| BseNI | ACTGG | 2 cut(s) | 382, 1839 |
| BseRI | GAGGAG | 3 cut(s) | 36, 1091, 1137 |
| BseSI | GKGCMC | 3 cut(s) | 426, 928, 953 |
| BseX3I | CGGCCG | 1 cut(s) | 154 |
| BseXI | GCAGC | 5 cut(s) | 69, 94, 581, 1070, 1168 |
| BseYI | CCCAGC | 2 cut(s) | 1325, 1847 |
| BsgI | GTGCAG | 1 cut(s) | 920 |
| Bsh1285I | CGRYCG | 1 cut(s) | 157 |
| BshFI | GGCC | 3 cut(s) | 156, 1010, 1296 |
| BshNI | GGYRCC | 3 cut(s) | 469, 799, 1800 |
| BsiEI | CGRYCG | 1 cut(s) | 157 |
| BsiHKAI | GWGCWC | 3 cut(s) | 206, 928, 1221 |
| BsiSI | CCGG | 5 cut(s) | 153, 185, 485, 554, 1222 |
| BslFI | GGGAC | 3 cut(s) | 118, 152, 1269 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 163, 188, 1025, 1292, 1424, 1739, 1810, 1854 |
| BsmAI | GTCTC | 2 cut(s) | 48, 1890 |
| BsmBI | CGTCTC | 1 cut(s) | 48 |
| BsmFI | GGGAC | 3 cut(s) | 118, 152, 1269 |
| BsmI | GAATGC | 2 cut(s) | 1443, 1523 |
| BsnI | GGCC | 3 cut(s) | 156, 1010, 1296 |
| Bsp119I | TTCGAA | 1 cut(s) | 814 |
| Bsp1286I | GDGCHC | 5 cut(s) | 206, 426, 928, 953, 1221 |
| Bsp1407I | TGTACA | 1 cut(s) | 1892 |
| Bsp143I | GATC | 6 cut(s) | 105, 175, 841, 1188, 1516, 1877 |
| BspANI | GGCC | 3 cut(s) | 156, 1010, 1296 |
| BspCNI | CTCAG | 3 cut(s) | 542, 1165, 1234 |
| BspLI | GGNNCC | 8 cut(s) | 133, 134, 168, 216, 471, 595, 801, 1802 |
| BspPI | GGATC | 3 cut(s) | 100, 849, 1872 |
| BspT104I | TTCGAA | 1 cut(s) | 814 |
| BspT107I | GGYRCC | 3 cut(s) | 469, 799, 1800 |
| BspTI | CTTAAG | 1 cut(s) | 1100 |
| BsrDI | GCAATG | 1 cut(s) | 712 |
| BsrFI | RCCGGY | 2 cut(s) | 152, 553 |
| BsrGI | TGTACA | 1 cut(s) | 1892 |
| BsrI | ACTGG | 2 cut(s) | 382, 1839 |
| BssAI | RCCGGY | 2 cut(s) | 152, 553 |
| BssECI | CCNNGG | 4 cut(s) | 135, 441, 573, 597 |
| BssMI | GATC | 6 cut(s) | 105, 175, 841, 1188, 1516, 1877 |
| BssNI | GRCGYC | 2 cut(s) | 41, 92 |
| BssSI | CACGAG | 1 cut(s) | 732 |
| Bst2BI | CACGAG | 1 cut(s) | 732 |
| Bst2UI | CCWGG | 3 cut(s) | 137, 442, 1899 |
| Bst4CI | ACNGT | 3 cut(s) | 258, 867, 1658 |
| Bst6I | CTCTTC | 2 cut(s) | 342, 894 |
| BstACI | GRCGYC | 2 cut(s) | 41, 92 |
| BstAFI | CTTAAG | 1 cut(s) | 1100 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1529 |
| BstAUI | TGTACA | 1 cut(s) | 1892 |
| BstBI | TTCGAA | 1 cut(s) | 814 |
| BstC8I | GCNNGC | 3 cut(s) | 121, 1008, 1294 |
| BstDEI | CTNAG | 6 cut(s) | 529, 914, 1118, 1173, 1242, 1510 |
| BstDSI | CCRYGG | 1 cut(s) | 573 |
| BstF5I | GGATG | 5 cut(s) | 284, 333, 583, 1613, 1942 |
| BstHHI | GCGC | 1 cut(s) | 553 |
| BstKTI | GATC | 6 cut(s) | 108, 178, 844, 1191, 1519, 1880 |
| BstMAI | GTCTC | 2 cut(s) | 48, 1890 |
| BstMBI | GATC | 6 cut(s) | 105, 175, 841, 1188, 1516, 1877 |
| BstMCI | CGRYCG | 1 cut(s) | 157 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 22, 91, 153, 1012, 1178, 1529 |
| BstNI | CCWGG | 3 cut(s) | 137, 442, 1899 |
| BstNSI | RCATGY | 1 cut(s) | 728 |
| BstSCI | CCNGG | 5 cut(s) | 135, 184, 440, 484, 1897 |
| BstSFI | CTRYAG | 1 cut(s) | 1390 |
| BstSLI | GKGCMC | 3 cut(s) | 426, 928, 953 |
| BstV1I | GCAGC | 5 cut(s) | 69, 94, 581, 1070, 1168 |
| BstV2I | GAAGAC | 3 cut(s) | 705, 1271, 1652 |
| BstX2I | RGATCY | 2 cut(s) | 841, 1877 |
| BstYI | RGATCY | 2 cut(s) | 841, 1877 |
| BstZI | CGGCCG | 1 cut(s) | 154 |
| BsuRI | GGCC | 3 cut(s) | 156, 1010, 1296 |
| BtgI | CCRYGG | 1 cut(s) | 573 |
| BtsCI | GGATG | 5 cut(s) | 284, 333, 583, 1613, 1942 |
| BtsIMutI | CAGTG | 3 cut(s) | 375, 1239, 1846 |
| Cac8I | GCNNGC | 3 cut(s) | 121, 1008, 1294 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1051 |
| CfoI | GCGC | 1 cut(s) | 553 |
| Cfr10I | RCCGGY | 2 cut(s) | 152, 553 |
| Cfr13I | GGNCC | 4 cut(s) | 132, 139, 166, 1008 |
| CseI | GACGC | 3 cut(s) | 30, 100, 665 |
| Csp6I | GTAC | 4 cut(s) | 470, 1127, 1398, 1893 |
| CviAII | CATG | 5 cut(s) | 725, 896, 1012, 1289, 1567 |
| CviQI | GTAC | 4 cut(s) | 470, 1127, 1398, 1893 |
| DdeI | CTNAG | 6 cut(s) | 529, 914, 1118, 1173, 1242, 1510 |
| DpnI | GATC | 6 cut(s) | 107, 177, 843, 1190, 1518, 1879 |
| DpnII | GATC | 6 cut(s) | 105, 175, 841, 1188, 1516, 1877 |
| DraIII | CACNNNGTG | 1 cut(s) | 898 |
| DriI | GACNNNNNGTC | 1 cut(s) | 130 |
| EaeI | YGGCCR | 1 cut(s) | 154 |
| EagI | CGGCCG | 1 cut(s) | 154 |
| Eam1104I | CTCTTC | 2 cut(s) | 342, 894 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 130 |
| EarI | CTCTTC | 2 cut(s) | 342, 894 |
| EciI | GGCGGA | 2 cut(s) | 347, 1826 |
| Ecl136II | GAGCTC | 2 cut(s) | 204, 1219 |
| EclXI | CGGCCG | 1 cut(s) | 154 |
| Eco24I | GRGCYC | 2 cut(s) | 206, 1221 |
| Eco32I | GATATC | 2 cut(s) | 991, 1836 |
| Eco47I | GGWCC | 3 cut(s) | 132, 139, 166 |
| Eco52I | CGGCCG | 1 cut(s) | 154 |
| Eco53kI | GAGCTC | 2 cut(s) | 204, 1219 |
| Eco57I | CTGAAG | 1 cut(s) | 1028 |
| EcoICRI | GAGCTC | 2 cut(s) | 204, 1219 |
| EcoO109I | RGGNCCY | 1 cut(s) | 132 |
| EcoRI | GAATTC | 1 cut(s) | 1700 |
| EcoRII | CCWGG | 3 cut(s) | 135, 440, 1897 |
| EcoRV | GATATC | 2 cut(s) | 991, 1836 |
| EcoT38I | GRGCYC | 2 cut(s) | 206, 1221 |
| Esp3I | CGTCTC | 1 cut(s) | 48 |
| FaeI | CATG | 5 cut(s) | 728, 899, 1015, 1292, 1570 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 398, 430 |
| FaqI | GGGAC | 3 cut(s) | 118, 152, 1269 |
| FatI | CATG | 5 cut(s) | 724, 895, 1011, 1288, 1566 |
| FauI | CCCGC | 3 cut(s) | 112, 178, 999 |
| FauNDI | CATATG | 1 cut(s) | 1853 |
| FbaI | TGATCA | 1 cut(s) | 1516 |
| Fnu4HI | GCNGC | 9 cut(s) | 11, 14, 58, 83, 157, 570, 1084, 1182, 1804 |
| FokI | GGATG | 4 cut(s) | 271, 340, 590, 1600 |
| FriOI | GRGCYC | 2 cut(s) | 206, 1221 |
| Fsp4HI | GCNGC | 9 cut(s) | 11, 14, 58, 83, 157, 570, 1084, 1182, 1804 |
| FspBI | CTAG | 2 cut(s) | 429, 1214 |
| GlaI | GCGC | 1 cut(s) | 552 |
| GluI | GCNGC | 9 cut(s) | 11, 14, 58, 83, 157, 570, 1084, 1182, 1804 |
| GsaI | CCCAGC | 2 cut(s) | 1329, 1851 |
| HaeIII | GGCC | 3 cut(s) | 156, 1010, 1296 |
| HapII | CCGG | 5 cut(s) | 153, 185, 485, 554, 1222 |
| HgaI | GACGC | 3 cut(s) | 30, 100, 665 |
| HhaI | GCGC | 1 cut(s) | 553 |
| Hin1I | GRCGYC | 2 cut(s) | 41, 92 |
| Hin1II | CATG | 5 cut(s) | 728, 899, 1015, 1292, 1570 |
| Hin6I | GCGC | 1 cut(s) | 551 |
| HinP1I | GCGC | 1 cut(s) | 551 |
| HincII | GTYRAC | 4 cut(s) | 21, 1332, 1409, 1561 |
| HindII | GTYRAC | 4 cut(s) | 21, 1332, 1409, 1561 |
| HindIII | AAGCTT | 2 cut(s) | 959, 1718 |
| HpaII | CCGG | 5 cut(s) | 153, 185, 485, 554, 1222 |
| HphI | GGTGA | 4 cut(s) | 676, 884, 1616, 1829 |
| Hpy166II | GTNNAC | 8 cut(s) | 21, 248, 926, 1239, 1332, 1409, 1561, 1895 |
| Hpy188I | TCNGA | 9 cut(s) | 198, 532, 601, 796, 1047, 1159, 1502, 1539, 1738 |
| Hpy188III | TCNNGA | 3 cut(s) | 429, 734, 1073 |
| Hpy8I | GTNNAC | 8 cut(s) | 21, 248, 926, 1239, 1332, 1409, 1561, 1895 |
| Hpy99I | CGWCG | 1 cut(s) | 90 |
| HpyAV | CCTTC | 5 cut(s) | 171, 329, 1052, 1743, 1808 |
| HpyCH4III | ACNGT | 3 cut(s) | 258, 867, 1658 |
| HpyCH4V | TGCA | 4 cut(s) | 569, 901, 926, 1532 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 22, 91, 153, 1012, 1178, 1529 |
| HpyF3I | CTNAG | 6 cut(s) | 529, 914, 1118, 1173, 1242, 1510 |
| Hsp92I | GRCGYC | 2 cut(s) | 41, 92 |
| Hsp92II | CATG | 5 cut(s) | 728, 899, 1015, 1292, 1570 |
| HspAI | GCGC | 1 cut(s) | 551 |
| KflI | GGGWCCC | 1 cut(s) | 132 |
| KpnI | GGTACC | 1 cut(s) | 473 |
| Ksp22I | TGATCA | 1 cut(s) | 1516 |
| Kzo9I | GATC | 6 cut(s) | 105, 175, 841, 1188, 1516, 1877 |
| LmnI | GCTCC | 4 cut(s) | 220, 593, 1224, 1827 |
| Lsp1109I | GCAGC | 5 cut(s) | 69, 94, 581, 1070, 1168 |
| MaeI | CTAG | 2 cut(s) | 429, 1214 |
| MaeIII | GTNAC | 4 cut(s) | 734, 1305, 1431, 1604 |
| MalI | GATC | 6 cut(s) | 107, 177, 843, 1190, 1518, 1879 |
| MboI | GATC | 6 cut(s) | 105, 175, 841, 1188, 1516, 1877 |
| MboII | GAAGA | 8 cut(s) | 359, 705, 881, 967, 1271, 1652, 1832, 1880 |
| MfeI | CAATTG | 1 cut(s) | 1001 |
| MflI | RGATCY | 2 cut(s) | 841, 1877 |
| MhlI | GDGCHC | 5 cut(s) | 206, 426, 928, 953, 1221 |
| MlyI | GAGTC | 7 cut(s) | 59, 202, 787, 884, 1042, 1631, 1769 |
| MmeI | TCCRAC | 2 cut(s) | 166, 1364 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1278 |
| MseI | TTAA | 8 cut(s) | 260, 672, 752, 1034, 1101, 1299, 1476, 1623 |
| MspA1I | CMGCKG | 1 cut(s) | 119 |
| MspCI | CTTAAG | 1 cut(s) | 1100 |
| MspI | CCGG | 5 cut(s) | 153, 185, 485, 554, 1222 |
| MspR9I | CCNGG | 5 cut(s) | 137, 186, 442, 486, 1899 |
| MunI | CAATTG | 1 cut(s) | 1001 |
| Mva1269I | GAATGC | 2 cut(s) | 1443, 1523 |
| MvaI | CCWGG | 3 cut(s) | 137, 442, 1899 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 22, 91, 153, 1012, 1178, 1529 |
| NciI | CCSGG | 2 cut(s) | 186, 486 |
| NdeI | CATATG | 1 cut(s) | 1853 |
| NdeII | GATC | 6 cut(s) | 105, 175, 841, 1188, 1516, 1877 |
| NlaIII | CATG | 5 cut(s) | 728, 899, 1015, 1292, 1570 |
| NlaIV | GGNNCC | 8 cut(s) | 133, 134, 168, 216, 471, 595, 801, 1802 |
| NmuCI | GTSAC | 2 cut(s) | 734, 1604 |
| NspI | RCATGY | 1 cut(s) | 728 |
| NspV | TTCGAA | 1 cut(s) | 814 |
| PciI | ACATGT | 1 cut(s) | 724 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 14 |
| PctI | GAATGC | 2 cut(s) | 1443, 1523 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1278 |
| PfeI | GAWTC | 4 cut(s) | 5, 455, 658, 884 |
| PflFI | GACNNNGTC | 2 cut(s) | 68, 1051 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1025, 1854 |
| PkrI | GCNGC | 9 cut(s) | 12, 15, 59, 84, 158, 571, 1085, 1183, 1805 |
| PleI | GAGTC | 7 cut(s) | 59, 201, 786, 883, 1042, 1631, 1768 |
| PpsI | GAGTC | 7 cut(s) | 59, 201, 786, 883, 1042, 1631, 1768 |
| PpuMI | RGGWCCY | 1 cut(s) | 132 |
| PscI | ACATGT | 1 cut(s) | 724 |
| Psp124BI | GAGCTC | 2 cut(s) | 206, 1221 |
| Psp5II | RGGWCCY | 1 cut(s) | 132 |
| Psp6I | CCWGG | 3 cut(s) | 135, 440, 1897 |
| PspFI | CCCAGC | 2 cut(s) | 1325, 1847 |
| PspGI | CCWGG | 3 cut(s) | 135, 440, 1897 |
| PspN4I | GGNNCC | 8 cut(s) | 133, 134, 168, 216, 471, 595, 801, 1802 |
| PspPI | GGNCC | 4 cut(s) | 132, 139, 166, 1008 |
| PspPPI | RGGWCCY | 1 cut(s) | 132 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1051 |
| PsuI | RGATCY | 2 cut(s) | 841, 1877 |
| PsyI | GACNNNGTC | 2 cut(s) | 68, 1051 |
| RsaI | GTAC | 4 cut(s) | 471, 1128, 1399, 1894 |
| RsaNI | GTAC | 4 cut(s) | 470, 1127, 1398, 1893 |
| SacI | GAGCTC | 2 cut(s) | 206, 1221 |
| SaqAI | TTAA | 8 cut(s) | 260, 672, 752, 1034, 1101, 1299, 1476, 1623 |
| SatI | GCNGC | 9 cut(s) | 11, 14, 58, 83, 157, 570, 1084, 1182, 1804 |
| Sau3AI | GATC | 6 cut(s) | 105, 175, 841, 1188, 1516, 1877 |
| Sau96I | GGNCC | 4 cut(s) | 132, 139, 166, 1008 |
| ScaI | AGTACT | 1 cut(s) | 1128 |
| SchI | GAGTC | 7 cut(s) | 59, 202, 787, 884, 1042, 1631, 1769 |
| ScrFI | CCNGG | 5 cut(s) | 137, 186, 442, 486, 1899 |
| SduI | GDGCHC | 5 cut(s) | 206, 426, 928, 953, 1221 |
| SfcI | CTRYAG | 1 cut(s) | 1390 |
| SfuI | TTCGAA | 1 cut(s) | 814 |
| SgrAI | CRCCGGYG | 1 cut(s) | 553 |
| SinI | GGWCC | 3 cut(s) | 132, 139, 166 |
| SmlI | CTYRAG | 4 cut(s) | 1100, 1544, 1688, 1757 |
| SmoI | CTYRAG | 4 cut(s) | 1100, 1544, 1688, 1757 |
| SspMI | CTAG | 2 cut(s) | 429, 1214 |
| SstI | GAGCTC | 2 cut(s) | 206, 1221 |
| StyD4I | CCNGG | 5 cut(s) | 135, 184, 440, 484, 1897 |
| TaaI | ACNGT | 3 cut(s) | 258, 867, 1658 |
| TaqI | TCGA | 4 cut(s) | 781, 814, 1072, 1191 |
| TaqII | GACCGA | 1 cut(s) | 154 |
| TatI | WGTACW | 3 cut(s) | 1126, 1397, 1892 |
| TauI | GCSGC | 4 cut(s) | 13, 16, 159, 1806 |
| TfiI | GAWTC | 4 cut(s) | 5, 455, 658, 884 |
| Tru1I | TTAA | 8 cut(s) | 260, 672, 752, 1034, 1101, 1299, 1476, 1623 |
| Tru9I | TTAA | 8 cut(s) | 260, 672, 752, 1034, 1101, 1299, 1476, 1623 |
| TscAI | CASTG | 3 cut(s) | 382, 1246, 1846 |
| TseFI | GTSAC | 2 cut(s) | 734, 1604 |
| TseI | GCWGC | 5 cut(s) | 57, 82, 569, 1083, 1181 |
| Tsp45I | GTSAC | 2 cut(s) | 734, 1604 |
| TspDTI | ATGAA | 2 cut(s) | 1481, 1756 |
| TspGWI | ACGGA | 3 cut(s) | 58, 103, 277 |
| TspRI | CASTG | 3 cut(s) | 382, 1246, 1846 |
| Tth111I | GACNNNGTC | 2 cut(s) | 68, 1051 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1025, 1854 |
| Vha464I | CTTAAG | 1 cut(s) | 1100 |
| VneI | GTGCAC | 1 cut(s) | 924 |
| VpaK11BI | GGWCC | 3 cut(s) | 132, 139, 166 |
| XapI | RAATTY | 4 cut(s) | 667, 772, 1105, 1700 |
| XbaI | TCTAGA | 1 cut(s) | 428 |
| XceI | RCATGY | 1 cut(s) | 728 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1671 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1278 |
| XspI | CTAG | 2 cut(s) | 429, 1214 |
| ZrmI | AGTACT | 1 cut(s) | 1128 |