RLG00000013941

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000546
Physical Location & Seq
Reverse (-)
44527903 .. 44531454
3552 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013941

Sequence Viewer

Length: 615 bp
ATGAACCCACAGCGGCCGCCGCCGCCCTATGTGTTCCTCAGTTCCGGAGGTTACGCCATGGGCAATCTGTACACTGCTCTGGTTGAAGAGGGCATACCTACAATTCGAGATAATGAAAAGCTGGAGTTGGGATCTGGGCTGAGCTCAGAAGAGAAGGAAAAAATGTTTGAGAAGACGTTGCTTCCACAACACTGCACATCAATTGAAGAATCGAAGCTTGTGATTGTCATCATTTCAATAAATTATCTGAATTCGAGCTGGTGCTTGGAGCAGGTTGCCAAGGCTGTTGAATGCAACAAACTAATACTCCCTGTTTTCGATGATGTGGATCCAATTGAACTCCGACTTCAAGAGGGATCAGTTGCGGAAGCTTTCTTCAAGCACGAAGAAACCTTTAAAGACAACCCAGACAAGGTGCGAAAATGGAGAGAAGCTATAAATCGCGTACCCATATCGGTTGGAGTTGCGGCAGCTTTCTTCAACAACAAAGAAGCCTTCAAAGACAACCCAGATAAGGCGCGAAAATGGAGAGAAGCTATATATCAAGGACCCATATCGGTAGGATGGTCTTTACAGTCTAGGGATGAATCCCAAGCTATTGTTGGAACATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

205

Amino Acids

23.02

Weight (kDa)

5.14

Isoelectric Point (pI)

48.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR_2 PF13676 65 - 121 3.5e-06 TIR domain
TIR PF01582 67 - 158 1.3e-18 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000546)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700
malus_domestica MD14G1049300.v1.1
rosa_chinensis RchiOBHm_Chr4g0433201 RchiOBHm_Chr6g0255111 RchiOBHm_Chr7g0235301 RchiOBHm_Chr7g0235351 RchiOBHm_Chr7g0235361 RchiOBHm_Chr7g0235381
rosa_laevigata RLG00000001164 RLG00000013941
rosa_multiflora Rmu_co8331033.1_g000001 Rmu_co8369635.1_g000001 Rmu_sc0000957.1_g000010 Rmu_sc0003304.1_g000033 Rmu_sc0003304.1_g000041 Rmu_sc0003488.1_g000001 Rmu_sc0004529.1_g000036 Rmu_sc0004637.1_g000013 Rmu_sc0004657.1_g000070 Rmu_sc0004657.1_g000076 Rmu_sc0006578.1_g000004 Rmu_sc0011027.1_g000008 Rmu_sc0011027.1_g000010 Rmu_sc0013813.1_g000001 Rmu_sc0014112.1_g000001 Rmu_sc0014112.1_g000002 Rmu_sc0014112.1_g000004 Rmu_ssc0000011.1_g000040 Rmu_ssc0000295.1_g000011
rosa_roxburghii Rroxscaffold_2G00103040 Rroxscaffold_3G00226100 Rroxscaffold_3G00226120 Rroxscaffold_5G00374710 Rroxscaffold_5G00374720 Rroxscaffold_7G00200160 Rroxscaffold_7G00200190
rosa_rugosa Rorug05G0474400 Rorug05G0474400 Rorug06G0037400 Rorug06G0037500 Rorug06G0037600 Rorug06G0038300 Rorug06G0038300 Rorug06G0038400 Rorug06G0038500 Rorug07G0289800 Rorug07G0289900 Rorug07G0290000 Rorug07G0290000
rosa_samantha Rh4AG320500 Rh4AG320700 Rh4AG321100 Rh4DG324200 Rh4DG324700 Rh6BG067500 Rh6CG066600 Rh6CG066700 Rh6DG063600 Rh6DG063700 Rh6DG147600 Rh7AG444800 Rh7AG445000 Rh7BG416700 Rh7BG416800 Rh7CG465200 Rh7CG465300 Rh7CG465700 Rh7DG433600 Rh7DG433800
rosa_wichuraiana Rw0G008730 Rw0G008770 Rw4G027830 Rw7G036940 Rw7G036980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 262
AccII CGCG 2 cut(s) 444, 520
AccIII TCCGGA 1 cut(s) 44
AciI CCGC 6 cut(s) 13, 17, 20, 23, 365, 467
AclWI GGATC 4 cut(s) 139, 323, 336, 364
AcoI YGGCCR 1 cut(s) 14
AcsI RAATTY 1 cut(s) 250
AfaI GTAC 2 cut(s) 71, 447
AfiI CCNNNNNNNGG 2 cut(s) 412, 514
AgsI TTSAA 9 cut(s) 86, 206, 237, 290, 338, 350, 379, 481, 499
AluBI AGCT 9 cut(s) 121, 144, 217, 258, 371, 434, 473, 536, 596
AluI AGCT 9 cut(s) 121, 144, 217, 258, 371, 434, 473, 536, 596
Alw21I GWGCWC 1 cut(s) 146
AlwI GGATC 4 cut(s) 139, 323, 336, 364
Aor13HI TCCGGA 1 cut(s) 44
AoxI GGCC 1 cut(s) 14
ApeKI GCWGC 1 cut(s) 470
ApoI RAATTY 1 cut(s) 250
AspLEI GCGC 1 cut(s) 520
AspS9I GGNCC 1 cut(s) 548
AvaII GGWCC 1 cut(s) 548
BamHI GGATCC 1 cut(s) 328
BanII GRGCYC 1 cut(s) 146
BarI GAAGNNNNNNTAC 2 cut(s) 78, 110
BbsI GAAGAC 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 146
BbvI GCAGC 1 cut(s) 482
BccI CCATC 1 cut(s) 558
BfaI CTAG 1 cut(s) 579
BfuAI ACCTGC 1 cut(s) 262
BisI GCNGC 6 cut(s) 14, 17, 20, 23, 468, 471
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 6 cut(s) 15, 18, 21, 24, 469, 472
Bme18I GGWCC 1 cut(s) 548
BmgT120I GGNCC 1 cut(s) 548
BmiI GGNNCC 2 cut(s) 330, 550
BpiI GAAGAC 1 cut(s) 179
BpmI CTGGAG 1 cut(s) 143
Bpu1102I GCTNAGC 1 cut(s) 140
BsaBI GATNNNNATC 2 cut(s) 227, 327
BsaJI CCNNGG 2 cut(s) 57, 279
BsaWI WCCGGW 1 cut(s) 44
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
Bsc4I CCNNNNNNNGG 2 cut(s) 412, 514
Bse8I GATNNNNATC 2 cut(s) 227, 327
BseAI TCCGGA 1 cut(s) 44
BseDI CCNNGG 2 cut(s) 57, 279
BseGI GGATG 2 cut(s) 569, 589
BseJI GATNNNNATC 2 cut(s) 227, 327
BseLI CCNNNNNNNGG 2 cut(s) 412, 514
BseMII CTCAG 3 cut(s) 52, 131, 159
BseX3I CGGCCG 1 cut(s) 14
BseXI GCAGC 1 cut(s) 482
BsgI GTGCAG 1 cut(s) 178
Bsh1236I CGCG 2 cut(s) 444, 520
Bsh1285I CGRYCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 16
BsiEI CGRYCG 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 146
BsiSI CCGG 1 cut(s) 45
BslI CCNNNNNNNGG 2 cut(s) 412, 514
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 146
Bsp13I TCCGGA 1 cut(s) 44
Bsp1407I TGTACA 1 cut(s) 69
Bsp143I GATC 3 cut(s) 131, 328, 356
Bsp1720I GCTNAGC 1 cut(s) 140
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 6 cut(s) 13, 17, 20, 23, 365, 467
BspANI GGCC 1 cut(s) 16
BspCNI CTCAG 3 cut(s) 51, 132, 158
BspEI TCCGGA 1 cut(s) 44
BspFNI CGCG 2 cut(s) 444, 520
BspLI GGNNCC 2 cut(s) 330, 550
BspMI ACCTGC 1 cut(s) 262
BspPI GGATC 4 cut(s) 139, 323, 336, 364
BsrGI TGTACA 1 cut(s) 69
BssECI CCNNGG 2 cut(s) 57, 279
BssMI GATC 3 cut(s) 131, 328, 356
BssT1I CCWWGG 2 cut(s) 57, 279
Bst4CI ACNGT 1 cut(s) 576
Bst6I CTCTTC 2 cut(s) 81, 144
BstAUI TGTACA 1 cut(s) 69
BstDEI CTNAG 3 cut(s) 38, 140, 145
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 2 cut(s) 569, 589
BstFNI CGCG 2 cut(s) 444, 520
BstHHI GCGC 1 cut(s) 520
BstKTI GATC 3 cut(s) 134, 331, 359
BstMBI GATC 3 cut(s) 131, 328, 356
BstMCI CGRYCG 1 cut(s) 17
BstMWI GCNNNNNNNGC 2 cut(s) 19, 22
BstUI CGCG 2 cut(s) 444, 520
BstV1I GCAGC 1 cut(s) 482
BstV2I GAAGAC 1 cut(s) 179
BstX2I RGATCY 2 cut(s) 131, 328
BstYI RGATCY 2 cut(s) 131, 328
BstZI CGGCCG 1 cut(s) 14
BsuRI GGCC 1 cut(s) 16
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 2 cut(s) 569, 589
BtsI GCAGTG 2 cut(s) 72, 190
BtsIMutI CAGTG 2 cut(s) 72, 190
BveI ACCTGC 1 cut(s) 262
CciNI GCGGCCGC 1 cut(s) 14
CfoI GCGC 1 cut(s) 520
Cfr13I GGNCC 1 cut(s) 548
Csp6I GTAC 2 cut(s) 70, 446
CviAII CATG 1 cut(s) 58
CviQI GTAC 2 cut(s) 70, 446
DdeI CTNAG 3 cut(s) 38, 140, 145
DpnI GATC 3 cut(s) 133, 330, 358
DpnII GATC 3 cut(s) 131, 328, 356
DraI TTTAAA 1 cut(s) 397
EaeI YGGCCR 1 cut(s) 14
EagI CGGCCG 1 cut(s) 14
Eam1104I CTCTTC 2 cut(s) 81, 144
EarI CTCTTC 2 cut(s) 81, 144
Ecl136II GAGCTC 1 cut(s) 144
EclXI CGGCCG 1 cut(s) 14
Eco130I CCWWGG 2 cut(s) 57, 279
Eco24I GRGCYC 1 cut(s) 146
Eco47I GGWCC 1 cut(s) 548
Eco52I CGGCCG 1 cut(s) 14
Eco53kI GAGCTC 1 cut(s) 144
EcoICRI GAGCTC 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 548
EcoRI GAATTC 1 cut(s) 250
EcoT14I CCWWGG 2 cut(s) 57, 279
EcoT38I GRGCYC 1 cut(s) 146
ErhI CCWWGG 2 cut(s) 57, 279
FaeI CATG 1 cut(s) 61
FaiI YATR 8 cut(s) 30, 59, 95, 437, 452, 539, 541, 554
FatI CATG 1 cut(s) 57
Fnu4HI GCNGC 6 cut(s) 14, 17, 20, 23, 468, 471
FokI GGATG 2 cut(s) 576, 596
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 6 cut(s) 14, 17, 20, 23, 468, 471
FspBI CTAG 1 cut(s) 579
GlaI GCGC 1 cut(s) 519
GluI GCNGC 6 cut(s) 14, 17, 20, 23, 468, 471
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 1 cut(s) 16
HapII CCGG 1 cut(s) 45
HhaI GCGC 1 cut(s) 520
Hin1II CATG 1 cut(s) 61
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HindIII AAGCTT 2 cut(s) 215, 369
HinfI GANTC 2 cut(s) 209, 587
HpaII CCGG 1 cut(s) 45
Hpy166II GTNNAC 1 cut(s) 72
Hpy188I TCNGA 3 cut(s) 148, 249, 344
Hpy188III TCNNGA 3 cut(s) 45, 107, 350
Hpy8I GTNNAC 1 cut(s) 72
HpyAV CCTTC 2 cut(s) 148, 505
HpyCH4III ACNGT 1 cut(s) 576
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 2 cut(s) 195, 294
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 22
HpyF3I CTNAG 3 cut(s) 38, 140, 145
HpySE526I ACGT 1 cut(s) 176
Hsp92II CATG 1 cut(s) 61
HspAI GCGC 1 cut(s) 518
Kpn2I TCCGGA 1 cut(s) 44
Kzo9I GATC 3 cut(s) 131, 328, 356
LmnI GCTCC 1 cut(s) 268
LpnPI CCDG 9 cut(s) 58, 65, 107, 120, 244, 257, 324, 420, 522
Lsp1109I GCAGC 1 cut(s) 482
MaeI CTAG 1 cut(s) 579
MaeII ACGT 1 cut(s) 176
MaeIII GTNAC 1 cut(s) 50
MalI GATC 3 cut(s) 133, 330, 358
MboI GATC 3 cut(s) 131, 328, 356
MboII GAAGA 7 cut(s) 98, 161, 184, 218, 367, 398, 469
MfeI CAATTG 2 cut(s) 201, 333
MflI RGATCY 2 cut(s) 131, 328
MhlI GDGCHC 1 cut(s) 146
MluCI AATT 5 cut(s) 102, 201, 241, 250, 333
MmeI TCCRAC 3 cut(s) 367, 439, 583
MnlI CCTC 4 cut(s) 41, 47, 82, 346
MroI TCCGGA 1 cut(s) 44
MseI TTAA 1 cut(s) 396
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 1 cut(s) 45
MunI CAATTG 2 cut(s) 201, 333
Mva1269I GAATGC 1 cut(s) 296
MvnI CGCG 2 cut(s) 444, 520
MwoI GCNNNNNNNGC 2 cut(s) 19, 22
NcoI CCATGG 1 cut(s) 57
NdeII GATC 3 cut(s) 131, 328, 356
NlaIII CATG 1 cut(s) 61
NlaIV GGNNCC 2 cut(s) 330, 550
NotI GCGGCCGC 1 cut(s) 14
PctI GAATGC 1 cut(s) 296
PfeI GAWTC 2 cut(s) 209, 587
PkrI GCNGC 6 cut(s) 15, 18, 21, 24, 469, 472
PpuMI RGGWCCY 1 cut(s) 548
Psp124BI GAGCTC 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 548
PspN4I GGNNCC 2 cut(s) 330, 550
PspPI GGNCC 1 cut(s) 548
PspPPI RGGWCCY 1 cut(s) 548
PsuI RGATCY 2 cut(s) 131, 328
RsaI GTAC 2 cut(s) 71, 447
RsaNI GTAC 2 cut(s) 70, 446
SacI GAGCTC 1 cut(s) 146
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 6 cut(s) 14, 17, 20, 23, 468, 471
Sau3AI GATC 3 cut(s) 131, 328, 356
Sau96I GGNCC 1 cut(s) 548
SduI GDGCHC 1 cut(s) 146
SinI GGWCC 1 cut(s) 548
Sse9I AATT 5 cut(s) 102, 201, 241, 250, 333
SsiI CCGC 6 cut(s) 13, 17, 20, 23, 365, 467
SspMI CTAG 1 cut(s) 579
SstI GAGCTC 1 cut(s) 146
StyI CCWWGG 2 cut(s) 57, 279
TaaI ACNGT 1 cut(s) 576
TaiI ACGT 1 cut(s) 179
TaqI TCGA 4 cut(s) 106, 212, 254, 318
TasI AATT 5 cut(s) 102, 201, 241, 250, 333
TatI WGTACW 1 cut(s) 69
TauI GCSGC 5 cut(s) 16, 19, 22, 25, 470
TfiI GAWTC 2 cut(s) 209, 587
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TscAI CASTG 2 cut(s) 79, 197
TseI GCWGC 1 cut(s) 470
TspDTI ATGAA 3 cut(s) 17, 129, 600
TspRI CASTG 2 cut(s) 79, 197
VpaK11BI GGWCC 1 cut(s) 548
XapI RAATTY 1 cut(s) 250
XcmI CCANNNNNNNNNTGG 1 cut(s) 599
XspI CTAG 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.