ADP binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 413 |
| AccIII | TCCGGA | 1 cut(s) | 99 |
| AciI | CCGC | 2 cut(s) | 57, 73 |
| AclWI | GGATC | 3 cut(s) | 115, 245, 258 |
| AcoI | YGGCCR | 2 cut(s) | 259, 538 |
| AcuI | CTGAAG | 1 cut(s) | 415 |
| AfaI | GTAC | 2 cut(s) | 299, 423 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 413 |
| AgsI | TTSAA | 2 cut(s) | 13, 316 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 130, 162 |
| AluBI | AGCT | 3 cut(s) | 437, 494, 503 |
| AluI | AGCT | 3 cut(s) | 437, 494, 503 |
| Alw21I | GWGCWC | 1 cut(s) | 170 |
| AlwI | GGATC | 3 cut(s) | 115, 245, 258 |
| Aor13HI | TCCGGA | 1 cut(s) | 99 |
| AoxI | GGCC | 3 cut(s) | 216, 259, 538 |
| ApeKI | GCWGC | 4 cut(s) | 395, 494, 497, 500 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 142 |
| AspS9I | GGNCC | 3 cut(s) | 91, 339, 416 |
| AsuHPI | GGTGA | 1 cut(s) | 61 |
| AvaII | GGWCC | 3 cut(s) | 91, 339, 416 |
| AxyI | CCTNAGG | 1 cut(s) | 220 |
| BalI | TGGCCA | 1 cut(s) | 540 |
| BamHI | GGATCC | 1 cut(s) | 250 |
| BbsI | GAAGAC | 1 cut(s) | 24 |
| Bbv12I | GWGCWC | 1 cut(s) | 170 |
| BbvI | GCAGC | 4 cut(s) | 382, 481, 484, 512 |
| BccI | CCATC | 2 cut(s) | 330, 364 |
| BceAI | ACGGC | 1 cut(s) | 49 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 69, 103 |
| BciVI | GTATCC | 1 cut(s) | 353 |
| BfaI | CTAG | 1 cut(s) | 111 |
| BfmI | CTRYAG | 1 cut(s) | 498 |
| BfuI | GTATCC | 1 cut(s) | 353 |
| BisI | GCNGC | 4 cut(s) | 396, 495, 498, 501 |
| BlsI | GCNGC | 4 cut(s) | 397, 496, 499, 502 |
| Bme18I | GGWCC | 3 cut(s) | 91, 339, 416 |
| BmgT120I | GGNCC | 3 cut(s) | 91, 339, 416 |
| BmiI | GGNNCC | 1 cut(s) | 252 |
| BmsI | GCATC | 1 cut(s) | 36 |
| BpiI | GAAGAC | 1 cut(s) | 24 |
| BpuEI | CTTGAG | 1 cut(s) | 278 |
| BsaJI | CCNNGG | 2 cut(s) | 45, 149 |
| BsaWI | WCCGGW | 1 cut(s) | 99 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 130, 160 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 413 |
| Bse118I | RCCGGY | 1 cut(s) | 53 |
| Bse21I | CCTNAGG | 1 cut(s) | 220 |
| BseAI | TCCGGA | 1 cut(s) | 99 |
| BseDI | CCNNGG | 2 cut(s) | 45, 149 |
| BseGI | GGATG | 1 cut(s) | 341 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 413 |
| BseXI | GCAGC | 4 cut(s) | 382, 481, 484, 512 |
| BseYI | CCCAGC | 1 cut(s) | 61 |
| BshFI | GGCC | 3 cut(s) | 218, 261, 540 |
| BsiHKAI | GWGCWC | 1 cut(s) | 170 |
| BsiSI | CCGG | 2 cut(s) | 54, 100 |
| BslFI | GGGAC | 1 cut(s) | 294 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 413 |
| BsmFI | GGGAC | 1 cut(s) | 294 |
| BsnI | GGCC | 3 cut(s) | 218, 261, 540 |
| Bsp1286I | GDGCHC | 1 cut(s) | 170 |
| Bsp13I | TCCGGA | 1 cut(s) | 99 |
| Bsp143I | GATC | 2 cut(s) | 107, 250 |
| BspACI | CCGC | 2 cut(s) | 57, 73 |
| BspANI | GGCC | 3 cut(s) | 218, 261, 540 |
| BspEI | TCCGGA | 1 cut(s) | 99 |
| BspLI | GGNNCC | 1 cut(s) | 252 |
| BspMAI | CTGCAG | 1 cut(s) | 502 |
| BspPI | GGATC | 3 cut(s) | 115, 245, 258 |
| BsrFI | RCCGGY | 1 cut(s) | 53 |
| BssAI | RCCGGY | 1 cut(s) | 53 |
| BssECI | CCNNGG | 2 cut(s) | 45, 149 |
| BssMI | GATC | 2 cut(s) | 107, 250 |
| BssT1I | CCWWGG | 1 cut(s) | 149 |
| Bst4CI | ACNGT | 2 cut(s) | 365, 387 |
| Bst6I | CTCTTC | 1 cut(s) | 311 |
| BstC8I | GCNNGC | 1 cut(s) | 59 |
| BstDEI | CTNAG | 1 cut(s) | 220 |
| BstDSI | CCRYGG | 1 cut(s) | 45 |
| BstEII | GGTNACC | 1 cut(s) | 49 |
| BstF5I | GGATG | 1 cut(s) | 341 |
| BstKTI | GATC | 2 cut(s) | 110, 253 |
| BstMBI | GATC | 2 cut(s) | 107, 250 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 33, 63, 72, 500, 525 |
| BstPI | GGTNACC | 1 cut(s) | 49 |
| BstSFI | CTRYAG | 1 cut(s) | 498 |
| BstV1I | GCAGC | 4 cut(s) | 382, 481, 484, 512 |
| BstV2I | GAAGAC | 1 cut(s) | 24 |
| BstX2I | RGATCY | 2 cut(s) | 107, 250 |
| BstYI | RGATCY | 2 cut(s) | 107, 250 |
| Bsu36I | CCTNAGG | 1 cut(s) | 220 |
| BsuI | GTATCC | 1 cut(s) | 353 |
| BsuRI | GGCC | 3 cut(s) | 218, 261, 540 |
| BtgI | CCRYGG | 1 cut(s) | 45 |
| BtsCI | GGATG | 1 cut(s) | 341 |
| BtsI | GCAGTG | 1 cut(s) | 461 |
| BtsIMutI | CAGTG | 1 cut(s) | 461 |
| Cac8I | GCNNGC | 1 cut(s) | 59 |
| Cfr10I | RCCGGY | 1 cut(s) | 53 |
| Cfr13I | GGNCC | 3 cut(s) | 91, 339, 416 |
| Csp6I | GTAC | 2 cut(s) | 298, 422 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 337, 372 |
| CviAII | CATG | 5 cut(s) | 245, 255, 376, 506, 529 |
| CviQI | GTAC | 2 cut(s) | 298, 422 |
| DdeI | CTNAG | 1 cut(s) | 220 |
| DpnI | GATC | 2 cut(s) | 109, 252 |
| DpnII | GATC | 2 cut(s) | 107, 250 |
| EaeI | YGGCCR | 2 cut(s) | 259, 538 |
| Eam1104I | CTCTTC | 1 cut(s) | 311 |
| EarI | CTCTTC | 1 cut(s) | 311 |
| Eco130I | CCWWGG | 1 cut(s) | 149 |
| Eco147I | AGGCCT | 1 cut(s) | 218 |
| Eco47I | GGWCC | 3 cut(s) | 91, 339, 416 |
| Eco57I | CTGAAG | 1 cut(s) | 415 |
| Eco81I | CCTNAGG | 1 cut(s) | 220 |
| Eco91I | GGTNACC | 1 cut(s) | 49 |
| EcoO65I | GGTNACC | 1 cut(s) | 49 |
| EcoT14I | CCWWGG | 1 cut(s) | 149 |
| ErhI | CCWWGG | 1 cut(s) | 149 |
| FaeI | CATG | 5 cut(s) | 248, 258, 379, 509, 532 |
| FaqI | GGGAC | 1 cut(s) | 294 |
| FatI | CATG | 5 cut(s) | 244, 254, 375, 505, 528 |
| FauI | CCCGC | 1 cut(s) | 50 |
| Fnu4HI | GCNGC | 4 cut(s) | 396, 495, 498, 501 |
| FokI | GGATG | 1 cut(s) | 348 |
| Fsp4HI | GCNGC | 4 cut(s) | 396, 495, 498, 501 |
| FspBI | CTAG | 1 cut(s) | 111 |
| GluI | GCNGC | 4 cut(s) | 396, 495, 498, 501 |
| GsaI | CCCAGC | 1 cut(s) | 65 |
| HaeIII | GGCC | 3 cut(s) | 218, 261, 540 |
| HapII | CCGG | 2 cut(s) | 54, 100 |
| Hin1II | CATG | 5 cut(s) | 248, 258, 379, 509, 532 |
| HinfI | GANTC | 1 cut(s) | 329 |
| HpaII | CCGG | 2 cut(s) | 54, 100 |
| HphI | GGTGA | 1 cut(s) | 61 |
| Hpy166II | GTNNAC | 1 cut(s) | 524 |
| Hpy188I | TCNGA | 3 cut(s) | 91, 137, 434 |
| Hpy188III | TCNNGA | 3 cut(s) | 100, 333, 407 |
| Hpy8I | GTNNAC | 1 cut(s) | 524 |
| Hpy99I | CGWCG | 1 cut(s) | 35 |
| HpyAV | CCTTC | 5 cut(s) | 17, 104, 413, 468, 469 |
| HpyCH4III | ACNGT | 2 cut(s) | 365, 387 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 278, 500 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 33, 63, 72, 500, 525 |
| HpyF3I | CTNAG | 1 cut(s) | 220 |
| Hsp92II | CATG | 5 cut(s) | 248, 258, 379, 509, 532 |
| Kpn2I | TCCGGA | 1 cut(s) | 99 |
| Kzo9I | GATC | 2 cut(s) | 107, 250 |
| LpnPI | CCDG | 6 cut(s) | 47, 67, 113, 318, 420, 432 |
| Lsp1109I | GCAGC | 4 cut(s) | 382, 481, 484, 512 |
| LweI | GCATC | 1 cut(s) | 36 |
| MaeI | CTAG | 1 cut(s) | 111 |
| MaeIII | GTNAC | 1 cut(s) | 49 |
| MalI | GATC | 2 cut(s) | 109, 252 |
| MboI | GATC | 2 cut(s) | 107, 250 |
| MboII | GAAGA | 5 cut(s) | 29, 328, 421, 436, 439 |
| MfeI | CAATTG | 1 cut(s) | 273 |
| MflI | RGATCY | 2 cut(s) | 107, 250 |
| MhlI | GDGCHC | 1 cut(s) | 170 |
| MlsI | TGGCCA | 1 cut(s) | 540 |
| MluCI | AATT | 1 cut(s) | 273 |
| MluNI | TGGCCA | 1 cut(s) | 540 |
| MlyI | GAGTC | 1 cut(s) | 323 |
| MnlI | CCTC | 6 cut(s) | 98, 143, 208, 241, 314, 509 |
| Mox20I | TGGCCA | 1 cut(s) | 540 |
| MroI | TCCGGA | 1 cut(s) | 99 |
| MroXI | GAANNNNTTC | 1 cut(s) | 142 |
| MscI | TGGCCA | 1 cut(s) | 540 |
| MslI | CAYNNNNRTG | 1 cut(s) | 44 |
| Msp20I | TGGCCA | 1 cut(s) | 540 |
| MspI | CCGG | 2 cut(s) | 54, 100 |
| MunI | CAATTG | 1 cut(s) | 273 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 33, 63, 72, 500, 525 |
| NdeII | GATC | 2 cut(s) | 107, 250 |
| NlaIII | CATG | 5 cut(s) | 248, 258, 379, 509, 532 |
| NlaIV | GGNNCC | 1 cut(s) | 252 |
| NmuCI | GTSAC | 1 cut(s) | 49 |
| PceI | AGGCCT | 1 cut(s) | 218 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 27 |
| PdmI | GAANNNNTTC | 1 cut(s) | 142 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 413 |
| PkrI | GCNGC | 4 cut(s) | 397, 496, 499, 502 |
| PleI | GAGTC | 1 cut(s) | 323 |
| PpsI | GAGTC | 1 cut(s) | 323 |
| PspEI | GGTNACC | 1 cut(s) | 49 |
| PspFI | CCCAGC | 1 cut(s) | 61 |
| PspN4I | GGNNCC | 1 cut(s) | 252 |
| PspPI | GGNCC | 3 cut(s) | 91, 339, 416 |
| PstI | CTGCAG | 1 cut(s) | 502 |
| PsuI | RGATCY | 2 cut(s) | 107, 250 |
| RsaI | GTAC | 2 cut(s) | 299, 423 |
| RsaNI | GTAC | 2 cut(s) | 298, 422 |
| RseI | CAYNNNNRTG | 1 cut(s) | 44 |
| SatI | GCNGC | 4 cut(s) | 396, 495, 498, 501 |
| Sau3AI | GATC | 2 cut(s) | 107, 250 |
| Sau96I | GGNCC | 3 cut(s) | 91, 339, 416 |
| SchI | GAGTC | 1 cut(s) | 323 |
| SduI | GDGCHC | 1 cut(s) | 170 |
| SetI | ASST | 8 cut(s) | 96, 135, 405, 421, 439, 480, 496, 505 |
| SfaNI | GCATC | 1 cut(s) | 36 |
| SfcI | CTRYAG | 1 cut(s) | 498 |
| SinI | GGWCC | 3 cut(s) | 91, 339, 416 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 44 |
| SmlI | CTYRAG | 1 cut(s) | 293 |
| SmoI | CTYRAG | 1 cut(s) | 293 |
| Sse9I | AATT | 1 cut(s) | 273 |
| SseBI | AGGCCT | 1 cut(s) | 218 |
| SsiI | CCGC | 2 cut(s) | 57, 73 |
| SspMI | CTAG | 1 cut(s) | 111 |
| StuI | AGGCCT | 1 cut(s) | 218 |
| StyI | CCWWGG | 1 cut(s) | 149 |
| TaaI | ACNGT | 2 cut(s) | 365, 387 |
| TaqI | TCGA | 2 cut(s) | 30, 121 |
| TasI | AATT | 1 cut(s) | 273 |
| TatI | WGTACW | 1 cut(s) | 297 |
| TscAI | CASTG | 1 cut(s) | 468 |
| TseFI | GTSAC | 1 cut(s) | 49 |
| TseI | GCWGC | 4 cut(s) | 395, 494, 497, 500 |
| Tsp45I | GTSAC | 1 cut(s) | 49 |
| TspDTI | ATGAA | 2 cut(s) | 30, 255 |
| TspGWI | ACGGA | 1 cut(s) | 34 |
| TspRI | CASTG | 1 cut(s) | 468 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 413 |
| VpaK11BI | GGWCC | 3 cut(s) | 91, 339, 416 |
| XmnI | GAANNNNTTC | 1 cut(s) | 142 |
| XspI | CTAG | 1 cut(s) | 111 |