ADP binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 820 |
| AccII | CGCG | 3 cut(s) | 182, 258, 494 |
| AccIII | TCCGGA | 1 cut(s) | 565 |
| AciI | CCGC | 3 cut(s) | 103, 205, 494 |
| AclWI | GGATC | 3 cut(s) | 61, 74, 102 |
| AcsI | RAATTY | 2 cut(s) | 430, 805 |
| AfaI | GTAC | 1 cut(s) | 185 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 150, 252, 385 |
| AgsI | TTSAA | 8 cut(s) | 28, 76, 88, 117, 219, 355, 454, 531 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 430, 462 |
| AluBI | AGCT | 8 cut(s) | 109, 172, 211, 274, 334, 466, 632, 660 |
| AluI | AGCT | 8 cut(s) | 109, 172, 211, 274, 334, 466, 632, 660 |
| Alw26I | GTCTC | 1 cut(s) | 493 |
| AlwI | GGATC | 3 cut(s) | 61, 74, 102 |
| Ama87I | CYCGRG | 1 cut(s) | 418 |
| Aor13HI | TCCGGA | 1 cut(s) | 565 |
| ApeKI | GCWGC | 1 cut(s) | 208 |
| ApoI | RAATTY | 2 cut(s) | 430, 805 |
| AspLEI | GCGC | 3 cut(s) | 258, 563, 821 |
| AspS9I | GGNCC | 1 cut(s) | 286 |
| AsuHPI | GGTGA | 2 cut(s) | 612, 615 |
| AvaI | CYCGRG | 1 cut(s) | 418 |
| AvaII | GGWCC | 1 cut(s) | 286 |
| BaeGI | GKGCMC | 1 cut(s) | 614 |
| BamHI | GGATCC | 1 cut(s) | 66 |
| BbsI | GAAGAC | 1 cut(s) | 842 |
| BbvI | GCAGC | 1 cut(s) | 220 |
| BccI | CCATC | 4 cut(s) | 296, 706, 777, 862 |
| BclI | TGATCA | 1 cut(s) | 602 |
| BcoDI | GTCTC | 1 cut(s) | 493 |
| BfaI | CTAG | 1 cut(s) | 317 |
| BisI | GCNGC | 2 cut(s) | 206, 209 |
| BlsI | GCNGC | 2 cut(s) | 207, 210 |
| Bme18I | GGWCC | 1 cut(s) | 286 |
| BmeT110I | CYCGRG | 1 cut(s) | 418 |
| BmgT120I | GGNCC | 1 cut(s) | 286 |
| BmiI | GGNNCC | 2 cut(s) | 68, 288 |
| BmrI | ACTGGG | 1 cut(s) | 394 |
| BmsI | GCATC | 3 cut(s) | 498, 501, 739 |
| BmuI | ACTGGG | 1 cut(s) | 394 |
| BpiI | GAAGAC | 1 cut(s) | 842 |
| BpmI | CTGGAG | 1 cut(s) | 669 |
| BpuEI | CTTGAG | 1 cut(s) | 452 |
| BsaBI | GATNNNNATC | 1 cut(s) | 65 |
| BsaJI | CCNNGG | 2 cut(s) | 17, 419 |
| BsaWI | WCCGGW | 1 cut(s) | 565 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 29, 59, 667, 697 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 150, 252, 385 |
| Bse1I | ACTGG | 2 cut(s) | 389, 686 |
| Bse8I | GATNNNNATC | 1 cut(s) | 65 |
| BseAI | TCCGGA | 1 cut(s) | 565 |
| BseDI | CCNNGG | 2 cut(s) | 17, 419 |
| BseGI | GGATG | 4 cut(s) | 307, 327, 717, 730 |
| BseJI | GATNNNNATC | 1 cut(s) | 65 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 150, 252, 385 |
| BseNI | ACTGG | 2 cut(s) | 389, 686 |
| BseSI | GKGCMC | 1 cut(s) | 614 |
| BseXI | GCAGC | 1 cut(s) | 220 |
| BseYI | CCCAGC | 1 cut(s) | 628 |
| BsgI | GTGCAG | 1 cut(s) | 359 |
| Bsh1236I | CGCG | 3 cut(s) | 182, 258, 494 |
| BsiHKCI | CYCGRG | 1 cut(s) | 418 |
| BsiSI | CCGG | 1 cut(s) | 566 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 150, 252, 385 |
| BsmAI | GTCTC | 1 cut(s) | 493 |
| BsmI | GAATGC | 1 cut(s) | 34 |
| BsoBI | CYCGRG | 1 cut(s) | 418 |
| Bsp1286I | GDGCHC | 1 cut(s) | 614 |
| Bsp13I | TCCGGA | 1 cut(s) | 565 |
| Bsp143I | GATC | 3 cut(s) | 66, 94, 602 |
| BspACI | CCGC | 3 cut(s) | 103, 205, 494 |
| BspEI | TCCGGA | 1 cut(s) | 565 |
| BspFNI | CGCG | 3 cut(s) | 182, 258, 494 |
| BspLI | GGNNCC | 2 cut(s) | 68, 288 |
| BspPI | GGATC | 3 cut(s) | 61, 74, 102 |
| BsrI | ACTGG | 2 cut(s) | 389, 686 |
| BssECI | CCNNGG | 2 cut(s) | 17, 419 |
| BssMI | GATC | 3 cut(s) | 66, 94, 602 |
| BssT1I | CCWWGG | 1 cut(s) | 17 |
| Bst4CI | ACNGT | 4 cut(s) | 314, 348, 537, 609 |
| Bst6I | CTCTTC | 1 cut(s) | 418 |
| BstC8I | GCNNGC | 1 cut(s) | 516 |
| BstDEI | CTNAG | 1 cut(s) | 656 |
| BstF5I | GGATG | 4 cut(s) | 307, 327, 717, 730 |
| BstFNI | CGCG | 3 cut(s) | 182, 258, 494 |
| BstHHI | GCGC | 3 cut(s) | 258, 563, 821 |
| BstKTI | GATC | 3 cut(s) | 69, 97, 605 |
| BstMAI | GTCTC | 1 cut(s) | 493 |
| BstMBI | GATC | 3 cut(s) | 66, 94, 602 |
| BstSLI | GKGCMC | 1 cut(s) | 614 |
| BstUI | CGCG | 3 cut(s) | 182, 258, 494 |
| BstV1I | GCAGC | 1 cut(s) | 220 |
| BstV2I | GAAGAC | 1 cut(s) | 842 |
| BstX2I | RGATCY | 1 cut(s) | 66 |
| BstYI | RGATCY | 1 cut(s) | 66 |
| BtgZI | GCGATG | 1 cut(s) | 475 |
| BtsCI | GGATG | 4 cut(s) | 307, 327, 717, 730 |
| BtsIMutI | CAGTG | 4 cut(s) | 382, 533, 605, 693 |
| Cac8I | GCNNGC | 1 cut(s) | 516 |
| CfoI | GCGC | 3 cut(s) | 258, 563, 821 |
| Cfr13I | GGNCC | 1 cut(s) | 286 |
| Csp6I | GTAC | 1 cut(s) | 184 |
| CviAII | CATG | 1 cut(s) | 442 |
| CviQI | GTAC | 1 cut(s) | 184 |
| DdeI | CTNAG | 1 cut(s) | 656 |
| DpnI | GATC | 3 cut(s) | 68, 96, 604 |
| DpnII | GATC | 3 cut(s) | 66, 94, 602 |
| DraI | TTTAAA | 3 cut(s) | 135, 237, 581 |
| Eam1104I | CTCTTC | 1 cut(s) | 418 |
| EarI | CTCTTC | 1 cut(s) | 418 |
| Eco130I | CCWWGG | 1 cut(s) | 17 |
| Eco47I | GGWCC | 1 cut(s) | 286 |
| Eco88I | CYCGRG | 1 cut(s) | 418 |
| EcoO109I | RGGNCCY | 1 cut(s) | 286 |
| EcoRI | GAATTC | 1 cut(s) | 430 |
| EcoT14I | CCWWGG | 1 cut(s) | 17 |
| ErhI | CCWWGG | 1 cut(s) | 17 |
| FaeI | CATG | 1 cut(s) | 445 |
| FatI | CATG | 1 cut(s) | 441 |
| FauNDI | CATATG | 1 cut(s) | 752 |
| FbaI | TGATCA | 1 cut(s) | 602 |
| Fnu4HI | GCNGC | 2 cut(s) | 206, 209 |
| FokI | GGATG | 4 cut(s) | 314, 334, 717, 724 |
| Fsp4HI | GCNGC | 2 cut(s) | 206, 209 |
| FspBI | CTAG | 1 cut(s) | 317 |
| FspI | TGCGCA | 1 cut(s) | 820 |
| GlaI | GCGC | 3 cut(s) | 257, 562, 820 |
| GluI | GCNGC | 2 cut(s) | 206, 209 |
| GsaI | CCCAGC | 1 cut(s) | 632 |
| GsuI | CTGGAG | 1 cut(s) | 669 |
| HapII | CCGG | 1 cut(s) | 566 |
| HhaI | GCGC | 3 cut(s) | 258, 563, 821 |
| Hin1II | CATG | 1 cut(s) | 445 |
| Hin6I | GCGC | 3 cut(s) | 256, 561, 819 |
| HinP1I | GCGC | 3 cut(s) | 256, 561, 819 |
| HindIII | AAGCTT | 2 cut(s) | 107, 658 |
| HinfI | GANTC | 4 cut(s) | 325, 543, 736, 813 |
| HpaII | CCGG | 1 cut(s) | 566 |
| HphI | GGTGA | 2 cut(s) | 612, 615 |
| Hpy188I | TCNGA | 3 cut(s) | 82, 735, 803 |
| Hpy188III | TCNNGA | 3 cut(s) | 88, 435, 566 |
| HpyAV | CCTTC | 1 cut(s) | 662 |
| HpyCH4III | ACNGT | 4 cut(s) | 314, 348, 537, 609 |
| HpyCH4V | TGCA | 3 cut(s) | 32, 376, 701 |
| HpyF3I | CTNAG | 1 cut(s) | 656 |
| Hsp92II | CATG | 1 cut(s) | 445 |
| HspAI | GCGC | 3 cut(s) | 256, 561, 819 |
| Kpn2I | TCCGGA | 1 cut(s) | 565 |
| Ksp22I | TGATCA | 1 cut(s) | 602 |
| Kzo9I | GATC | 3 cut(s) | 66, 94, 602 |
| LmnI | GCTCC | 4 cut(s) | 6, 412, 568, 688 |
| Lsp1109I | GCAGC | 1 cut(s) | 220 |
| LweI | GCATC | 3 cut(s) | 498, 501, 739 |
| MaeI | CTAG | 1 cut(s) | 317 |
| MaeIII | GTNAC | 2 cut(s) | 424, 621 |
| MalI | GATC | 3 cut(s) | 68, 96, 604 |
| MboI | GATC | 3 cut(s) | 66, 94, 602 |
| MboII | GAAGA | 9 cut(s) | 105, 136, 207, 367, 405, 520, 627, 823, 842 |
| MfeI | CAATTG | 3 cut(s) | 71, 454, 678 |
| MflI | RGATCY | 1 cut(s) | 66 |
| MhlI | GDGCHC | 1 cut(s) | 614 |
| MluCI | AATT | 6 cut(s) | 71, 358, 430, 454, 678, 805 |
| MlyI | GAGTC | 1 cut(s) | 552 |
| MmeI | TCCRAC | 3 cut(s) | 105, 177, 321 |
| MnlI | CCTC | 5 cut(s) | 84, 389, 414, 421, 591 |
| MroI | TCCGGA | 1 cut(s) | 565 |
| MseI | TTAA | 5 cut(s) | 134, 236, 521, 580, 809 |
| MspI | CCGG | 1 cut(s) | 566 |
| MunI | CAATTG | 3 cut(s) | 71, 454, 678 |
| Mva1269I | GAATGC | 1 cut(s) | 34 |
| MvnI | CGCG | 3 cut(s) | 182, 258, 494 |
| NdeI | CATATG | 1 cut(s) | 752 |
| NdeII | GATC | 3 cut(s) | 66, 94, 602 |
| NlaIII | CATG | 1 cut(s) | 445 |
| NlaIV | GGNNCC | 2 cut(s) | 68, 288 |
| NmuCI | GTSAC | 1 cut(s) | 621 |
| NsbI | TGCGCA | 1 cut(s) | 820 |
| PctI | GAATGC | 1 cut(s) | 34 |
| PfeI | GAWTC | 3 cut(s) | 325, 736, 813 |
| PkrI | GCNGC | 2 cut(s) | 207, 210 |
| PleI | GAGTC | 1 cut(s) | 551 |
| PpsI | GAGTC | 1 cut(s) | 551 |
| PpuMI | RGGWCCY | 1 cut(s) | 286 |
| Psp5II | RGGWCCY | 1 cut(s) | 286 |
| PspFI | CCCAGC | 1 cut(s) | 628 |
| PspN4I | GGNNCC | 2 cut(s) | 68, 288 |
| PspPI | GGNCC | 1 cut(s) | 286 |
| PspPPI | RGGWCCY | 1 cut(s) | 286 |
| PsuI | RGATCY | 1 cut(s) | 66 |
| RsaI | GTAC | 1 cut(s) | 185 |
| RsaNI | GTAC | 1 cut(s) | 184 |
| SaqAI | TTAA | 5 cut(s) | 134, 236, 521, 580, 809 |
| SatI | GCNGC | 2 cut(s) | 206, 209 |
| Sau3AI | GATC | 3 cut(s) | 66, 94, 602 |
| Sau96I | GGNCC | 1 cut(s) | 286 |
| SchI | GAGTC | 1 cut(s) | 552 |
| SduI | GDGCHC | 1 cut(s) | 614 |
| SfaNI | GCATC | 3 cut(s) | 498, 501, 739 |
| SinI | GGWCC | 1 cut(s) | 286 |
| SmlI | CTYRAG | 1 cut(s) | 467 |
| SmoI | CTYRAG | 1 cut(s) | 467 |
| Sse9I | AATT | 6 cut(s) | 71, 358, 430, 454, 678, 805 |
| SsiI | CCGC | 3 cut(s) | 103, 205, 494 |
| SspI | AATATT | 1 cut(s) | 697 |
| SspMI | CTAG | 1 cut(s) | 317 |
| StyI | CCWWGG | 1 cut(s) | 17 |
| TaaI | ACNGT | 4 cut(s) | 314, 348, 537, 609 |
| TaqI | TCGA | 1 cut(s) | 56 |
| TasI | AATT | 6 cut(s) | 71, 358, 430, 454, 678, 805 |
| TauI | GCSGC | 1 cut(s) | 208 |
| TfiI | GAWTC | 3 cut(s) | 325, 736, 813 |
| Tru1I | TTAA | 5 cut(s) | 134, 236, 521, 580, 809 |
| Tru9I | TTAA | 5 cut(s) | 134, 236, 521, 580, 809 |
| TscAI | CASTG | 4 cut(s) | 389, 540, 612, 693 |
| TseFI | GTSAC | 1 cut(s) | 621 |
| TseI | GCWGC | 1 cut(s) | 208 |
| Tsp45I | GTSAC | 1 cut(s) | 621 |
| TspDTI | ATGAA | 2 cut(s) | 338, 740 |
| TspRI | CASTG | 4 cut(s) | 389, 540, 612, 693 |
| VpaK11BI | GGWCC | 1 cut(s) | 286 |
| XapI | RAATTY | 2 cut(s) | 430, 805 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 337 |
| XspI | CTAG | 1 cut(s) | 317 |