RLG00000001164

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000546
Physical Location & Seq
Reverse (-)
11146895 .. 11147530
636 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001164

Sequence Viewer

Length: 636 bp
ATGGTTTCAGCATCATTTAACTCTTGCCAGAATCGAACTGGCATATGGGGGCAAATTAGCCCTCTTAAGCTTAACATGTCCGACCGAACGGACTTCCAAGTCGAAGGCGAGTTCGAACTAATATGCAGGAATTTAGGCTTCTCGGGCCCAGACCAGTTTGCTATCCCCGCCGCCACCTGGGAGACCCAGTCGACGAACTCTGATCTTCTCCCCCCCTACCGGATGGAAGGGCCCGAGAGTGTGAAACCCGAGGAAGTGAAGCACCCAAAGGAGGTCGAGGAAGAGGAGAAATGGGAGATGGGTCTGCAGTCACGTCGACTGCTTAAGCCGCCGCCGCCGATGAGTGTTCCGGTGATTGACAATGCATGCTCGACATGGGACATTATGAGGGGCTTAGGGCCCCAAGGTGAAACTTCTGATGAAGAAGTAGAAGAAGAGGATAATTTGATTACTTCTGATGAAGATGAAGAAGATCGTATTTCGATTAAAGAGATTCTACCGAGCTCCGGGTATTCTTTTACTACTTGGAATGACGATGACTCTTCCAGCACTACCACAGACTCCTCCACCATTTCTTCTTCGGAAAAGGAAACCTCTCGCGGGTATTTACCCCTGTATGAAGGAATTTCCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

212

Amino Acids

23.64

Weight (kDa)

4.2

Isoelectric Point (pI)

56.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000546)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_4g25760 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700 FvH4_5g34700
malus_domestica MD14G1049300.v1.1
rosa_chinensis RchiOBHm_Chr4g0433201 RchiOBHm_Chr6g0255111 RchiOBHm_Chr7g0235301 RchiOBHm_Chr7g0235351 RchiOBHm_Chr7g0235361 RchiOBHm_Chr7g0235381
rosa_laevigata RLG00000001164 RLG00000013941
rosa_multiflora Rmu_co8331033.1_g000001 Rmu_co8369635.1_g000001 Rmu_sc0000957.1_g000010 Rmu_sc0003304.1_g000033 Rmu_sc0003304.1_g000041 Rmu_sc0003488.1_g000001 Rmu_sc0004529.1_g000036 Rmu_sc0004637.1_g000013 Rmu_sc0004657.1_g000070 Rmu_sc0004657.1_g000076 Rmu_sc0006578.1_g000004 Rmu_sc0011027.1_g000008 Rmu_sc0011027.1_g000010 Rmu_sc0013813.1_g000001 Rmu_sc0014112.1_g000001 Rmu_sc0014112.1_g000002 Rmu_sc0014112.1_g000004 Rmu_ssc0000011.1_g000040 Rmu_ssc0000295.1_g000011
rosa_roxburghii Rroxscaffold_2G00103040 Rroxscaffold_3G00226100 Rroxscaffold_3G00226120 Rroxscaffold_5G00374710 Rroxscaffold_5G00374720 Rroxscaffold_7G00200160 Rroxscaffold_7G00200190
rosa_rugosa Rorug05G0474400 Rorug05G0474400 Rorug06G0037400 Rorug06G0037500 Rorug06G0037600 Rorug06G0038300 Rorug06G0038300 Rorug06G0038400 Rorug06G0038500 Rorug07G0289800 Rorug07G0289900 Rorug07G0290000 Rorug07G0290000
rosa_samantha Rh4AG320500 Rh4AG320700 Rh4AG321100 Rh4DG324200 Rh4DG324700 Rh6BG067500 Rh6CG066600 Rh6CG066700 Rh6DG063600 Rh6DG063700 Rh6DG147600 Rh7AG444800 Rh7AG445000 Rh7BG416700 Rh7BG416800 Rh7CG465200 Rh7CG465300 Rh7CG465700 Rh7DG433600 Rh7DG433800
rosa_wichuraiana Rw0G008730 Rw0G008770 Rw4G027830 Rw7G036940 Rw7G036980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
AccI GTMKAC 2 cut(s) 191, 316
AccII CGCG 1 cut(s) 600
AciI CCGC 6 cut(s) 168, 171, 329, 332, 335, 600
AcsI RAATTY 2 cut(s) 130, 624
AfiI CCNNNNNNNGG 5 cut(s) 177, 219, 271, 506, 600
AflII CTTAAG 2 cut(s) 65, 323
AflIII ACRYGT 1 cut(s) 75
AjiI CACGTC 1 cut(s) 314
AjnI CCWGG 1 cut(s) 176
AluBI AGCT 2 cut(s) 70, 504
AluI AGCT 2 cut(s) 70, 504
Alw21I GWGCWC 1 cut(s) 506
Alw26I GTCTC 1 cut(s) 176
Ama87I CYCGRG 3 cut(s) 142, 233, 248
AoxI GGCC 3 cut(s) 145, 230, 398
ApaI GGGCCC 3 cut(s) 149, 234, 402
ApoI RAATTY 2 cut(s) 130, 624
AspS9I GGNCC 6 cut(s) 145, 146, 230, 231, 398, 399
AsuC2I CCSGG 1 cut(s) 508
AsuHPI GGTGA 2 cut(s) 364, 419
AsuII TTCGAA 1 cut(s) 114
AvaI CYCGRG 3 cut(s) 142, 233, 248
BaeGI GKGCMC 3 cut(s) 149, 234, 402
BanII GRGCYC 4 cut(s) 149, 234, 402, 506
Bbv12I GWGCWC 1 cut(s) 506
BccI CCATC 2 cut(s) 217, 292
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 1 cut(s) 178
BcnI CCSGG 1 cut(s) 508
BcoDI GTCTC 1 cut(s) 176
BfmI CTRYAG 1 cut(s) 305
BfrI CTTAAG 2 cut(s) 65, 323
BisI GCNGC 4 cut(s) 171, 329, 332, 335
BlsI GCNGC 4 cut(s) 172, 330, 333, 336
Bme1390I CCNGG 2 cut(s) 178, 508
BmeT110I CYCGRG 3 cut(s) 142, 233, 248
BmgBI CACGTC 1 cut(s) 314
BmgT120I GGNCC 6 cut(s) 145, 146, 230, 231, 398, 399
BmiI GGNNCC 4 cut(s) 147, 232, 400, 401
BmrFI CCNGG 2 cut(s) 178, 508
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 1 cut(s) 20
BmuI ACTGGG 1 cut(s) 181
Bpu10I CCTNAGC 1 cut(s) 394
Bpu14I TTCGAA 1 cut(s) 114
BpuMI CCSGG 1 cut(s) 508
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 3 cut(s) 177, 249, 403
BsaWI WCCGGW 2 cut(s) 219, 349
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 5 cut(s) 177, 219, 271, 506, 600
Bse1I ACTGG 3 cut(s) 43, 154, 187
BseBI CCWGG 1 cut(s) 178
BseDI CCNNGG 3 cut(s) 177, 249, 403
BseGI GGATG 1 cut(s) 228
BseLI CCNNNNNNNGG 5 cut(s) 177, 219, 271, 506, 600
BseNI ACTGG 3 cut(s) 43, 154, 187
BseRI GAGGAG 2 cut(s) 299, 553
BseSI GKGCMC 3 cut(s) 149, 234, 402
Bsh1236I CGCG 1 cut(s) 600
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 3 cut(s) 147, 232, 400
BsiEI CGRYCG 1 cut(s) 85
BsiHKAI GWGCWC 1 cut(s) 506
BsiHKCI CYCGRG 3 cut(s) 142, 233, 248
BsiSI CCGG 3 cut(s) 220, 350, 507
BslFI GGGAC 1 cut(s) 392
BslI CCNNNNNNNGG 5 cut(s) 177, 219, 271, 506, 600
BsmAI GTCTC 1 cut(s) 176
BsmFI GGGAC 1 cut(s) 392
BsnI GGCC 3 cut(s) 147, 232, 400
Bso31I GGTCTC 1 cut(s) 176
BsoBI CYCGRG 3 cut(s) 142, 233, 248
Bsp119I TTCGAA 1 cut(s) 114
Bsp120I GGGCCC 3 cut(s) 145, 230, 398
Bsp1286I GDGCHC 4 cut(s) 149, 234, 402, 506
Bsp143I GATC 2 cut(s) 202, 472
BspACI CCGC 6 cut(s) 168, 171, 329, 332, 335, 600
BspANI GGCC 3 cut(s) 147, 232, 400
BspFNI CGCG 1 cut(s) 600
BspLI GGNNCC 4 cut(s) 147, 232, 400, 401
BspMAI CTGCAG 1 cut(s) 309
BspT104I TTCGAA 1 cut(s) 114
BspTI CTTAAG 2 cut(s) 65, 323
BspTNI GGTCTC 1 cut(s) 176
BsrI ACTGG 3 cut(s) 43, 154, 187
BssECI CCNNGG 3 cut(s) 177, 249, 403
BssMI GATC 2 cut(s) 202, 472
BssT1I CCWWGG 1 cut(s) 403
Bst2UI CCWGG 1 cut(s) 178
Bst6I CTCTTC 3 cut(s) 276, 429, 547
BstAFI CTTAAG 2 cut(s) 65, 323
BstBI TTCGAA 1 cut(s) 114
BstC8I GCNNGC 1 cut(s) 367
BstDEI CTNAG 1 cut(s) 394
BstF5I GGATG 1 cut(s) 228
BstFNI CGCG 1 cut(s) 600
BstKTI GATC 2 cut(s) 205, 475
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 2 cut(s) 202, 472
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 4 cut(s) 144, 167, 328, 334
BstNI CCWGG 1 cut(s) 178
BstNSI RCATGY 2 cut(s) 79, 369
BstSCI CCNGG 2 cut(s) 176, 506
BstSFI CTRYAG 1 cut(s) 305
BstSLI GKGCMC 3 cut(s) 149, 234, 402
BstUI CGCG 1 cut(s) 600
BsuRI GGCC 3 cut(s) 147, 232, 400
BtrI CACGTC 1 cut(s) 314
BtsCI GGATG 1 cut(s) 228
Cac8I GCNNGC 1 cut(s) 367
Cfr13I GGNCC 6 cut(s) 145, 146, 230, 231, 398, 399
CviAII CATG 3 cut(s) 76, 366, 375
CviJI RGCY 9 cut(s) 60, 70, 138, 147, 232, 328, 393, 400, 504
CviKI_1 RGCY 9 cut(s) 60, 70, 138, 147, 232, 328, 393, 400, 504
DdeI CTNAG 1 cut(s) 394
DpnI GATC 2 cut(s) 204, 474
DpnII GATC 2 cut(s) 202, 472
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
Eam1104I CTCTTC 3 cut(s) 276, 429, 547
EarI CTCTTC 3 cut(s) 276, 429, 547
Ecl136II GAGCTC 1 cut(s) 504
Eco130I CCWWGG 1 cut(s) 403
Eco24I GRGCYC 4 cut(s) 149, 234, 402, 506
Eco31I GGTCTC 1 cut(s) 176
Eco53kI GAGCTC 1 cut(s) 504
Eco88I CYCGRG 3 cut(s) 142, 233, 248
EcoICRI GAGCTC 1 cut(s) 504
EcoO109I RGGNCCY 3 cut(s) 230, 398, 399
EcoRII CCWGG 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 403
EcoT22I ATGCAT 1 cut(s) 367
EcoT38I GRGCYC 4 cut(s) 149, 234, 402, 506
ErhI CCWWGG 1 cut(s) 403
FaeI CATG 3 cut(s) 79, 369, 378
FaiI YATR 8 cut(s) 44, 46, 77, 124, 367, 376, 386, 618
FaqI GGGAC 1 cut(s) 392
FatI CATG 3 cut(s) 75, 365, 374
FauI CCCGC 2 cut(s) 175, 593
FauNDI CATATG 1 cut(s) 44
FblI GTMKAC 2 cut(s) 191, 316
Fnu4HI GCNGC 4 cut(s) 171, 329, 332, 335
FokI GGATG 1 cut(s) 235
FriOI GRGCYC 4 cut(s) 149, 234, 402, 506
Fsp4HI GCNGC 4 cut(s) 171, 329, 332, 335
GluI GCNGC 4 cut(s) 171, 329, 332, 335
HaeIII GGCC 3 cut(s) 147, 232, 400
HapII CCGG 3 cut(s) 220, 350, 507
Hin1II CATG 3 cut(s) 79, 369, 378
HincII GTYRAC 2 cut(s) 192, 317
HindII GTYRAC 2 cut(s) 192, 317
HindIII AAGCTT 1 cut(s) 68
HinfI GANTC 4 cut(s) 31, 493, 539, 560
HpaII CCGG 3 cut(s) 220, 350, 507
HphI GGTGA 2 cut(s) 364, 419
Hpy166II GTNNAC 2 cut(s) 192, 317
Hpy188I TCNGA 6 cut(s) 82, 202, 418, 457, 583, 631
Hpy8I GTNNAC 2 cut(s) 192, 317
Hpy99I CGWCG 2 cut(s) 196, 318
HpyAV CCTTC 3 cut(s) 98, 221, 614
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 3 cut(s) 126, 307, 365
HpyF10VI GCNNNNNNNGC 4 cut(s) 144, 167, 328, 334
HpyF3I CTNAG 1 cut(s) 394
HpySE526I ACGT 1 cut(s) 313
Hsp92II CATG 3 cut(s) 79, 369, 378
Kzo9I GATC 2 cut(s) 202, 472
LmnI GCTCC 1 cut(s) 509
LweI GCATC 1 cut(s) 20
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 1 cut(s) 309
MalI GATC 2 cut(s) 204, 474
MboI GATC 2 cut(s) 202, 472
MhlI GDGCHC 4 cut(s) 149, 234, 402, 506
MluCI AATT 4 cut(s) 54, 130, 442, 624
MlyI GAGTC 2 cut(s) 533, 554
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 9 cut(s) 72, 244, 265, 271, 277, 381, 430, 574, 604
Mph1103I ATGCAT 1 cut(s) 367
MseI TTAA 5 cut(s) 18, 66, 72, 324, 486
MspCI CTTAAG 2 cut(s) 65, 323
MspI CCGG 3 cut(s) 220, 350, 507
MspR9I CCNGG 2 cut(s) 178, 508
MvaI CCWGG 1 cut(s) 178
MvnI CGCG 1 cut(s) 600
MwoI GCNNNNNNNGC 4 cut(s) 144, 167, 328, 334
NciI CCSGG 1 cut(s) 508
NdeI CATATG 1 cut(s) 44
NdeII GATC 2 cut(s) 202, 472
NlaIII CATG 3 cut(s) 79, 369, 378
NlaIV GGNNCC 4 cut(s) 147, 232, 400, 401
NmuCI GTSAC 1 cut(s) 309
NsiI ATGCAT 1 cut(s) 367
NspI RCATGY 2 cut(s) 79, 369
NspV TTCGAA 1 cut(s) 114
PaeI GCATGC 1 cut(s) 369
PciI ACATGT 1 cut(s) 75
PfeI GAWTC 2 cut(s) 31, 493
PflFI GACNNNGTC 1 cut(s) 187
PkrI GCNGC 4 cut(s) 172, 330, 333, 336
PleI GAGTC 2 cut(s) 533, 554
PpsI GAGTC 2 cut(s) 533, 554
PscI ACATGT 1 cut(s) 75
Psp124BI GAGCTC 1 cut(s) 506
Psp6I CCWGG 1 cut(s) 176
PspGI CCWGG 1 cut(s) 176
PspN4I GGNNCC 4 cut(s) 147, 232, 400, 401
PspOMI GGGCCC 3 cut(s) 145, 230, 398
PspPI GGNCC 6 cut(s) 145, 146, 230, 231, 398, 399
PstI CTGCAG 1 cut(s) 309
PsyI GACNNNGTC 1 cut(s) 187
SacI GAGCTC 1 cut(s) 506
SalI GTCGAC 2 cut(s) 190, 315
SaqAI TTAA 5 cut(s) 18, 66, 72, 324, 486
SatI GCNGC 4 cut(s) 171, 329, 332, 335
Sau3AI GATC 2 cut(s) 202, 472
Sau96I GGNCC 6 cut(s) 145, 146, 230, 231, 398, 399
SchI GAGTC 2 cut(s) 533, 554
ScrFI CCNGG 2 cut(s) 178, 508
SduI GDGCHC 4 cut(s) 149, 234, 402, 506
SetI ASST 7 cut(s) 72, 179, 276, 316, 409, 506, 596
SfaNI GCATC 1 cut(s) 20
SfcI CTRYAG 1 cut(s) 305
SfuI TTCGAA 1 cut(s) 114
SmlI CTYRAG 2 cut(s) 65, 323
SmoI CTYRAG 2 cut(s) 65, 323
SphI GCATGC 1 cut(s) 369
Sse9I AATT 4 cut(s) 54, 130, 442, 624
SsiI CCGC 6 cut(s) 168, 171, 329, 332, 335, 600
SstI GAGCTC 1 cut(s) 506
StyD4I CCNGG 2 cut(s) 176, 506
StyI CCWWGG 1 cut(s) 403
TaiI ACGT 1 cut(s) 316
TaqI TCGA 8 cut(s) 34, 102, 114, 191, 276, 316, 371, 482
TaqII GACCGA 1 cut(s) 99
TasI AATT 4 cut(s) 54, 130, 442, 624
TauI GCSGC 4 cut(s) 173, 331, 334, 337
TfiI GAWTC 2 cut(s) 31, 493
Tru1I TTAA 5 cut(s) 18, 66, 72, 324, 486
Tru9I TTAA 5 cut(s) 18, 66, 72, 324, 486
TseFI GTSAC 1 cut(s) 309
Tsp45I GTSAC 1 cut(s) 309
TspDTI ATGAA 4 cut(s) 435, 474, 480, 633
TspGWI ACGGA 1 cut(s) 104
Tth111I GACNNNGTC 1 cut(s) 187
Vha464I CTTAAG 2 cut(s) 65, 323
XapI RAATTY 2 cut(s) 130, 624
XceI RCATGY 2 cut(s) 79, 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 35
XmiI GTMKAC 2 cut(s) 191, 316
Zsp2I ATGCAT 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.