Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0000573

Physical Location & Seq

Genomic Coordinates Forward (+)

9297143 .. 9301076

Gene Structure 3934 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g16330.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1152 bp

ATGGACCCCGCCGCACCTCCGCCTCCGCCGCCGCCGACACAACCGGAGACCACTTCCACCAGGCACCACTCGTACAGCCGGAAGCAGAAGTCTCTCGGCCTCTTATGCTCCAATTTCATCTCATTGTACGACCGCGAAGGCGTCACCTCCGTCGGCCTAGACGACGCCGCTTCAAAATTAGGAGTTGAGAGGCGGCGGATCTACGATATCGTTAATGTTCTTGAGAGCGTTGGGGTTCTTGCGCGAAAGGCGAAGAATCAATATTCTTGGAAAGGGTTTGGGGCAATCCCAGATACCTTGCAGGAGCTAAAGGAAGCCGGTCTGAAAGAGAACTTCAATACTTTCCACGGAAATTTCCAGGGCAGTGATGATCCAAAGTGTTCGCAGGGTTCGGATGATGAGGAAGGTGATGAGAGTGGTCCTGCGACCGGGAGTCAGAATGAGAATCCCGGTGCAAATCCAAAATCTTCAGCAAACTCGAAAAATGATAATAGAAGGGAAAAGTCTCTTGCACTTCTTACACAGAATTTTGTGAAGCTCTTTGTCTGCTCCACTGTAGAATCCATCTCCCTTGATGAAGCTGCGAAGTTATTACTTGGGGATGTACACAATGCATCTATAATGAGAACAGCTAAAGTAAGGCGGATTTATGATATTGCAAATGTTTTGTCCTCCATGAATCTGATTGAAAAGACCCACACAGCAGATACAAGGAAGCCTGCATACAAGTGGTTAGGATTGAGAGGAAAAGATGCCGCAAGTTCAGCTTCAGACGAGTCTAGAAAAAGAGCTTTTGGAACAGATATCACAAATGTCAGTTCTAAGAGGGGTAAAGGAGAATCTTCTACTACTGGGAAATTGGAAGCTCAGATACAAAAGCAACATGAGAGTACAGTAAGGCAACGTGAGAGCATAGTACGTACTGTTGATAAGAACAATTCAGAGGATTCAAGAGAAGGTACAAAGAGCTACCAGTTTGGTCCTTTTGCTCCAGTCACTATTGCCAGAGCTGGGAATTTCAACATGAAGAAAGTTGACTGGGACAATTTTTCATCTACTTACCGTCCCCAGTATCAGAATCAAGGAGAAGGAGGAAATACTGGGAGAAAGAGGAGAGATCAGGAAAGAGAGATAAACAGAACGGGAGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006355 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0019219 GO:0019222 GO:0022402 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032875 GO:0032876 GO:0034641 GO:0034645 GO:0042023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0045786 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051252 GO:0051726 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090329 GO:0097159 GO:0140110 GO:1901360 GO:1901363 GO:1901576 GO:1903506 GO:2000104 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E2F_TDP

Protein Analysis

384

Amino Acids

42.27

Weight (kDa)

9.11

Isoelectric Point (pI)

33.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_E2F_TDP	PF02319	28 - 118	9.7e-20	E2F/DP family winged-helix DNA-binding domain
WHD_E2F_TDP	PF02319	189 - 268	1.8e-19	E2F/DP family winged-helix DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000573)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48160 AT3G48160
fragaria_vesca	FvH4_3g21790 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330
malus_domestica	MD06G1181700.v1.1 MD14G1187600.v1.1
prunus_persica	Prupe.5G180000_v2.0.a1 Prupe.5G180000_v2.0.a1 Prupe.5G180000_v2.0.a1 Prupe.5G180000_v2.0.a1
pyrus_communis	pycom06g16070 pycom14g15550
rosa_chinensis	RchiOBHm_Chr2g0100091 RchiOBHm_Chr2g0131211 RchiOBHm_Chr2g0131231 RchiOBHm_Chr2g0155761 RchiOBHm_Chr5g0037341 RchiOBHm_Chr6g0286771 RchiOBHm_Chr7g0179801
rosa_laevigata	RLG00000005323 RLG00000033775
rosa_multiflora	Rmu_co8405049.1_g000002 Rmu_sc0003547.1_g000004 Rmu_sc0004156.1_g000008 Rmu_sc0007546.1_g000012 Rmu_sc0026307.1_g000001 Rmu_sc0032585.1_g000001 Rmu_ssc0000019.1_g000022
rosa_roxburghii	Rroxscaffold_1G00027620 Rroxscaffold_1G00027630 Rroxscaffold_1G00043630 Rroxscaffold_2G00107640 Rroxscaffold_3G00273460 Rroxscaffold_5G00347960 Rroxscaffold_5G00350580 Rroxscaffold_5G00366890 Rroxscaffold_6G00391730 Rroxscaffold_6G00392580 Rroxscaffold_7G00170270 Rroxscaffold_7G00170280
rosa_rugosa	Rorug02G0499800 Rorug04G0099000 Rorug04G0116000 Rorug05G0129300 Rorug05G0164200 Rorug06G0428300 Rorug06G0428400
rosa_samantha	Rh1DG306200 Rh2AG348600 Rh2CG139500 Rh2CG334400 Rh2CG334500 Rh2CG334700 Rh2DG139800 Rh2DG373800 Rh5BG255900 Rh5CG289000 Rh5DG264300 Rh5DG449300 Rh6AG295400 Rh6BG299400 Rh6CG299300 Rh6DG291200 Rh7AG028200 Rh7BG027100 Rh7BG173900 Rh7CG029000 Rh7DG028500
rosa_wichuraiana	Rw5G023500 Rw7G002240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	63
AccII	CGCG	2 cut(s)	135, 244
AclWI	GGATC	2 cut(s)	206, 365
AcsI	RAATTY	3 cut(s)	352, 526, 1015
AcuI	CTGAAG	2 cut(s)	453, 753
AcyI	GRCGYC	2 cut(s)	141, 165
AfaI	GTAC	7 cut(s)	74, 128, 606, 892, 918, 922, 961
AfiI	CCNNNNNNNGG	2 cut(s)	428, 1011
AgsI	TTSAA	5 cut(s)	174, 337, 689, 951, 1021
AhdI	GACNNNNNGTC	1 cut(s)	432
AjnI	CCWGG	2 cut(s)	59, 357
AluBI	AGCT	9 cut(s)	307, 538, 581, 632, 767, 791, 866, 969, 1010
AluI	AGCT	9 cut(s)	307, 538, 581, 632, 767, 791, 866, 969, 1010
Alw26I	GTCTC	4 cut(s)	41, 96, 510, 1140
AlwI	GGATC	2 cut(s)	206, 365
AoxI	GGCC	2 cut(s)	97, 154
ApeKI	GCWGC	1 cut(s)	581
ApoI	RAATTY	3 cut(s)	352, 526, 1015
ArsI	GACNNNNNNTTYG	2 cut(s)	653, 685
AspLEI	GCGC	1 cut(s)	244
AspS9I	GGNCC	3 cut(s)	4, 419, 980
AsuC2I	CCSGG	2 cut(s)	430, 450
AsuHPI	GGTGA	2 cut(s)	136, 419
AvaII	GGWCC	3 cut(s)	4, 419, 980
BanI	GGYRCC	1 cut(s)	63
BarI	GAAGNNNNNNTAC	4 cut(s)	707, 739, 855, 887
BbvI	GCAGC	1 cut(s)	568
BccI	CCATC	1 cut(s)	572
BciT130I	CCWGG	2 cut(s)	61, 359
BcnI	CCSGG	2 cut(s)	430, 450
BcoDI	GTCTC	4 cut(s)	41, 96, 510, 1140
BfaI	CTAG	3 cut(s)	158, 780, 1150
BfmI	CTRYAG	1 cut(s)	555
BisI	GCNGC	7 cut(s)	12, 29, 32, 168, 194, 582, 756
BlsI	GCNGC	7 cut(s)	13, 30, 33, 169, 195, 583, 757
Bme1390I	CCNGG	4 cut(s)	61, 359, 430, 450
Bme18I	GGWCC	3 cut(s)	4, 419, 980
BmeRI	GACNNNNNGTC	1 cut(s)	432
BmgT120I	GGNCC	3 cut(s)	4, 419, 980
BmiI	GGNNCC	2 cut(s)	6, 65
BmrFI	CCNGG	4 cut(s)	61, 359, 430, 450
BmrI	ACTGGG	4 cut(s)	861, 1048, 1063, 1110
BmsI	GCATC	2 cut(s)	623, 742
BmuI	ACTGGG	4 cut(s)	861, 1048, 1063, 1110
BpmI	CTGGAG	1 cut(s)	975
BpuEI	CTTGAG	1 cut(s)	242
BpuMI	CCSGG	2 cut(s)	430, 450
BsaAI	YACGTR	1 cut(s)	920
BsaHI	GRCGYC	2 cut(s)	141, 165
BsaI	GGTCTC	1 cut(s)	41
BsaJI	CCNNGG	2 cut(s)	346, 358
BsaWI	WCCGGW	1 cut(s)	43
Bsc4I	CCNNNNNNNGG	2 cut(s)	428, 1011
Bse118I	RCCGGY	1 cut(s)	317
Bse1I	ACTGG	6 cut(s)	856, 973, 992, 1043, 1069, 1105
BseBI	CCWGG	2 cut(s)	61, 359
BseDI	CCNNGG	2 cut(s)	346, 358
BseGI	GGATG	2 cut(s)	400, 607
BseLI	CCNNNNNNNGG	2 cut(s)	428, 1011
BseMII	CTCAG	1 cut(s)	881
BseNI	ACTGG	6 cut(s)	856, 973, 992, 1043, 1069, 1105
BseRI	GAGGAG	1 cut(s)	1126
BseXI	GCAGC	1 cut(s)	568
BseYI	CCCAGC	1 cut(s)	1010
Bsh1236I	CGCG	2 cut(s)	135, 244
Bsh1285I	CGRYCG	2 cut(s)	133, 429
BshFI	GGCC	2 cut(s)	99, 156
BshNI	GGYRCC	1 cut(s)	63
BsiEI	CGRYCG	2 cut(s)	133, 429
BsiSI	CCGG	5 cut(s)	44, 79, 318, 429, 450
BslFI	GGGAC	2 cut(s)	1050, 1055
BslI	CCNNNNNNNGG	2 cut(s)	428, 1011
BsmAI	GTCTC	4 cut(s)	41, 96, 510, 1140
BsmFI	GGGAC	2 cut(s)	1050, 1055
BsnI	GGCC	2 cut(s)	99, 156
Bso31I	GGTCTC	1 cut(s)	41
Bsp1407I	TGTACA	1 cut(s)	604
Bsp143I	GATC	3 cut(s)	198, 370, 1117
BspANI	GGCC	2 cut(s)	99, 156
BspCNI	CTCAG	1 cut(s)	880
BspFNI	CGCG	2 cut(s)	135, 244
BspLI	GGNNCC	2 cut(s)	6, 65
BspPI	GGATC	2 cut(s)	206, 365
BspT107I	GGYRCC	1 cut(s)	63
BspTNI	GGTCTC	1 cut(s)	41
BsrFI	RCCGGY	1 cut(s)	317
BsrGI	TGTACA	1 cut(s)	604
BsrI	ACTGG	6 cut(s)	856, 973, 992, 1043, 1069, 1105
BssAI	RCCGGY	1 cut(s)	317
BssECI	CCNNGG	2 cut(s)	346, 358
BssMI	GATC	3 cut(s)	198, 370, 1117
BssNI	GRCGYC	2 cut(s)	141, 165
Bst2UI	CCWGG	2 cut(s)	61, 359
Bst4CI	ACNGT	4 cut(s)	556, 895, 925, 1064
BstACI	GRCGYC	2 cut(s)	141, 165
BstAUI	TGTACA	1 cut(s)	604
BstBAI	YACGTR	1 cut(s)	920
BstC8I	GCNNGC	1 cut(s)	720
BstDEI	CTNAG	2 cut(s)	822, 867
BstDSI	CCRYGG	1 cut(s)	346
BstF5I	GGATG	2 cut(s)	400, 607
BstFNI	CGCG	2 cut(s)	135, 244
BstHHI	GCGC	1 cut(s)	244
BstKTI	GATC	3 cut(s)	201, 373, 1120
BstMAI	GTCTC	4 cut(s)	41, 96, 510, 1140
BstMBI	GATC	3 cut(s)	198, 370, 1117
BstMCI	CGRYCG	2 cut(s)	133, 429
BstMWI	GCNNNNNNNGC	4 cut(s)	28, 105, 248, 764
BstNI	CCWGG	2 cut(s)	61, 359
BstSCI	CCNGG	4 cut(s)	59, 357, 428, 448
BstSFI	CTRYAG	1 cut(s)	555
BstSNI	TACGTA	1 cut(s)	920
BstUI	CGCG	2 cut(s)	135, 244
BstV1I	GCAGC	1 cut(s)	568
BstX2I	RGATCY	1 cut(s)	198
BstYI	RGATCY	1 cut(s)	198
BsuRI	GGCC	2 cut(s)	99, 156
BtgI	CCRYGG	1 cut(s)	346
BtsCI	GGATG	2 cut(s)	400, 607
BtsI	GCAGTG	1 cut(s)	370
BtsIMutI	CAGTG	2 cut(s)	370, 552
Cac8I	GCNNGC	1 cut(s)	720
CfoI	GCGC	1 cut(s)	244
Cfr10I	RCCGGY	1 cut(s)	317
Cfr13I	GGNCC	3 cut(s)	4, 419, 980
CseI	GACGC	2 cut(s)	130, 173
Csp6I	GTAC	7 cut(s)	73, 127, 605, 891, 917, 921, 960
CviAII	CATG	3 cut(s)	676, 884, 1024
CviQI	GTAC	7 cut(s)	73, 127, 605, 891, 917, 921, 960
DdeI	CTNAG	2 cut(s)	822, 867
DpnI	GATC	3 cut(s)	200, 372, 1119
DpnII	GATC	3 cut(s)	198, 370, 1117
DriI	GACNNNNNGTC	1 cut(s)	432
Eam1105I	GACNNNNNGTC	1 cut(s)	432
EciI	GGCGGA	4 cut(s)	9, 15, 211, 658
Eco105I	TACGTA	1 cut(s)	920
Eco31I	GGTCTC	1 cut(s)	41
Eco32I	GATATC	2 cut(s)	208, 805
Eco47I	GGWCC	3 cut(s)	4, 419, 980
Eco57I	CTGAAG	2 cut(s)	453, 753
EcoRII	CCWGG	2 cut(s)	59, 357
EcoRV	GATATC	2 cut(s)	208, 805
EcoT22I	ATGCAT	1 cut(s)	616
FaeI	CATG	3 cut(s)	679, 887, 1027
FaiI	YATR	8 cut(s)	106, 620, 651, 677, 724, 885, 914, 1025
FalI	AAGNNNNNCTT	2 cut(s)	751, 783
FaqI	GGGAC	2 cut(s)	1050, 1055
FatI	CATG	3 cut(s)	675, 883, 1023
FauI	CCCGC	1 cut(s)	16
Fnu4HI	GCNGC	7 cut(s)	12, 29, 32, 168, 194, 582, 756
FokI	GGATG	2 cut(s)	407, 614
Fsp4HI	GCNGC	7 cut(s)	12, 29, 32, 168, 194, 582, 756
FspBI	CTAG	3 cut(s)	158, 780, 1150
GlaI	GCGC	1 cut(s)	243
GluI	GCNGC	7 cut(s)	12, 29, 32, 168, 194, 582, 756
GsaI	CCCAGC	1 cut(s)	1014
GsuI	CTGGAG	1 cut(s)	975
HaeIII	GGCC	2 cut(s)	99, 156
HapII	CCGG	5 cut(s)	44, 79, 318, 429, 450
HgaI	GACGC	2 cut(s)	130, 173
HhaI	GCGC	1 cut(s)	244
Hin1I	GRCGYC	2 cut(s)	141, 165
Hin1II	CATG	3 cut(s)	679, 887, 1027
Hin6I	GCGC	1 cut(s)	242
HinP1I	GCGC	1 cut(s)	242
HincII	GTYRAC	1 cut(s)	1036
HindII	GTYRAC	1 cut(s)	1036
HinfI	GANTC	9 cut(s)	256, 433, 445, 560, 679, 776, 839, 947, 1078
HpaII	CCGG	5 cut(s)	44, 79, 318, 429, 450
HphI	GGTGA	2 cut(s)	136, 419
Hpy166II	GTNNAC	2 cut(s)	607, 1036
Hpy188I	TCNGA	8 cut(s)	324, 394, 438, 684, 772, 870, 943, 1077
Hpy188III	TCNNGA	4 cut(s)	221, 780, 951, 1121
Hpy8I	GTNNAC	2 cut(s)	607, 1036
Hpy99I	CGWCG	2 cut(s)	155, 167
HpyAV	CCTTC	5 cut(s)	131, 398, 489, 950, 1082
HpyCH4III	ACNGT	4 cut(s)	556, 895, 925, 1064
HpyCH4IV	ACGT	2 cut(s)	904, 919
HpyCH4V	TGCA	6 cut(s)	301, 455, 512, 614, 659, 722
HpyF10VI	GCNNNNNNNGC	4 cut(s)	28, 105, 248, 764
HpyF3I	CTNAG	2 cut(s)	822, 867
HpySE526I	ACGT	2 cut(s)	904, 919
Hsp92I	GRCGYC	2 cut(s)	141, 165
Hsp92II	CATG	3 cut(s)	679, 887, 1027
HspAI	GCGC	1 cut(s)	242
Kzo9I	GATC	3 cut(s)	198, 370, 1117
LmnI	GCTCC	4 cut(s)	113, 304, 554, 994
Lsp1109I	GCAGC	1 cut(s)	568
LweI	GCATC	2 cut(s)	623, 742
MaeI	CTAG	3 cut(s)	158, 780, 1150
MaeII	ACGT	2 cut(s)	904, 919
MaeIII	GTNAC	2 cut(s)	142, 994
MalI	GATC	3 cut(s)	200, 372, 1119
MboI	GATC	3 cut(s)	198, 370, 1117
MboII	GAAGA	4 cut(s)	265, 459, 834, 1039
MflI	RGATCY	1 cut(s)	198
MluCI	AATT	8 cut(s)	112, 176, 352, 526, 857, 937, 1015, 1045
MlyI	GAGTC	2 cut(s)	442, 785
Mph1103I	ATGCAT	1 cut(s)	616
MseI	TTAA	1 cut(s)	213
MslI	CAYNNNNRTG	1 cut(s)	727
MspI	CCGG	5 cut(s)	44, 79, 318, 429, 450
MspR9I	CCNGG	4 cut(s)	61, 359, 430, 450
MvaI	CCWGG	2 cut(s)	61, 359
MvnI	CGCG	2 cut(s)	135, 244
MwoI	GCNNNNNNNGC	4 cut(s)	28, 105, 248, 764
NciI	CCSGG	2 cut(s)	430, 450
NdeII	GATC	3 cut(s)	198, 370, 1117
NlaIII	CATG	3 cut(s)	679, 887, 1027
NlaIV	GGNNCC	2 cut(s)	6, 65
NmeAIII	GCCGAG	1 cut(s)	75
NmuCI	GTSAC	2 cut(s)	142, 994
NsiI	ATGCAT	1 cut(s)	616
PcsI	WCGNNNNNNNCGW	1 cut(s)	159
PfeI	GAWTC	7 cut(s)	256, 445, 560, 679, 839, 947, 1078
PkrI	GCNGC	7 cut(s)	13, 30, 33, 169, 195, 583, 757
PleI	GAGTC	2 cut(s)	441, 784
PpsI	GAGTC	2 cut(s)	441, 784
Ppu21I	YACGTR	1 cut(s)	920
Psp6I	CCWGG	2 cut(s)	59, 357
PspFI	CCCAGC	1 cut(s)	1010
PspGI	CCWGG	2 cut(s)	59, 357
PspN4I	GGNNCC	2 cut(s)	6, 65
PspPI	GGNCC	3 cut(s)	4, 419, 980
PsuI	RGATCY	1 cut(s)	198
RsaI	GTAC	7 cut(s)	74, 128, 606, 892, 918, 922, 961
RsaNI	GTAC	7 cut(s)	73, 127, 605, 891, 917, 921, 960
RseI	CAYNNNNRTG	1 cut(s)	727
SaqAI	TTAA	1 cut(s)	213
SatI	GCNGC	7 cut(s)	12, 29, 32, 168, 194, 582, 756
Sau3AI	GATC	3 cut(s)	198, 370, 1117
Sau96I	GGNCC	3 cut(s)	4, 419, 980
SchI	GAGTC	2 cut(s)	442, 785
ScrFI	CCNGG	4 cut(s)	61, 359, 430, 450
SfaNI	GCATC	2 cut(s)	623, 742
SfcI	CTRYAG	1 cut(s)	555
SinI	GGWCC	3 cut(s)	4, 419, 980
SmiMI	CAYNNNNRTG	1 cut(s)	727
SmlI	CTYRAG	1 cut(s)	221
SmoI	CTYRAG	1 cut(s)	221
SnaBI	TACGTA	1 cut(s)	920
Sse9I	AATT	8 cut(s)	112, 176, 352, 526, 857, 937, 1015, 1045
SspI	AATATT	1 cut(s)	263
SspMI	CTAG	3 cut(s)	158, 780, 1150
StyD4I	CCNGG	4 cut(s)	59, 357, 428, 448
TaaI	ACNGT	4 cut(s)	556, 895, 925, 1064
TaiI	ACGT	2 cut(s)	907, 922
TaqI	TCGA	1 cut(s)	479
TasI	AATT	8 cut(s)	112, 176, 352, 526, 857, 937, 1015, 1045
TatI	WGTACW	2 cut(s)	604, 890
TauI	GCSGC	6 cut(s)	14, 31, 34, 170, 196, 758
TfiI	GAWTC	7 cut(s)	256, 445, 560, 679, 839, 947, 1078
Tru1I	TTAA	1 cut(s)	213
Tru9I	TTAA	1 cut(s)	213
TscAI	CASTG	2 cut(s)	370, 559
TseFI	GTSAC	2 cut(s)	142, 994
TseI	GCWGC	1 cut(s)	581
Tsp45I	GTSAC	2 cut(s)	142, 994
TspDTI	ATGAA	5 cut(s)	106, 591, 692, 1040, 1041
TspGWI	ACGGA	2 cut(s)	139, 363
TspRI	CASTG	2 cut(s)	370, 559
VpaK11BI	GGWCC	3 cut(s)	4, 419, 980
XapI	RAATTY	3 cut(s)	352, 526, 1015
XbaI	TCTAGA	1 cut(s)	779
XspI	CTAG	3 cut(s)	158, 780, 1150
Zsp2I	ATGCAT	1 cut(s)	616

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.