Rh5DG449300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000573
Physical Location & Seq
Forward (+)
71674983 .. 71676677
1695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG449300.1

Sequence Viewer

Length: 459 bp
ATGAAGAGGAGCTTTGGGACAGATCTAATGGATGCGAGTAGTAAGAGGAGCAAGGTTGTGGAGAAGAGAGTTCAAGATCAGGGTGAGGTTGAGAACTGGAATTCGGATGGCTGTGGGGCGAAGGAGAGTTCGAAGAGCTACCAGTTTGGTCCTTTCGCTCCGGGGGCGTTCCAAGAGCTAGGAAGGGTGTTGACATCAACAAGGTGGTGCTTGTCGACGGCTTCCATGACCTATGGCGGTGGGCATCTTCAGCAGGGAATCTCCTGGACCTGTGACGGCGTGGTGGCCATGGCGGTTTTCCGGCAGATTGGTGGGGCGGCGAGGATTTGGTGGTCTGGGTTGATCGCGGGGGCGACGTCGCTATGGGGAATCTGCTCGGAACGTGGCGGCTGCGTCTGGGCGGTGTTTCTGAGCGGATCAGTGGGGGAGGTCGCGGCAATCGTGGGTCGGGCAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

16.14

Weight (kDa)

8.69

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000573)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48160 AT3G48160
fragaria_vesca FvH4_3g21790 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330
malus_domestica MD06G1181700.v1.1 MD14G1187600.v1.1
prunus_persica Prupe.5G180000_v2.0.a1 Prupe.5G180000_v2.0.a1 Prupe.5G180000_v2.0.a1 Prupe.5G180000_v2.0.a1
pyrus_communis pycom06g16070 pycom14g15550
rosa_chinensis RchiOBHm_Chr2g0100091 RchiOBHm_Chr2g0131211 RchiOBHm_Chr2g0131231 RchiOBHm_Chr2g0155761 RchiOBHm_Chr5g0037341 RchiOBHm_Chr6g0286771 RchiOBHm_Chr7g0179801
rosa_laevigata RLG00000005323 RLG00000033775
rosa_multiflora Rmu_co8405049.1_g000002 Rmu_sc0003547.1_g000004 Rmu_sc0004156.1_g000008 Rmu_sc0007546.1_g000012 Rmu_sc0026307.1_g000001 Rmu_sc0032585.1_g000001 Rmu_ssc0000019.1_g000022
rosa_roxburghii Rroxscaffold_1G00027620 Rroxscaffold_1G00027630 Rroxscaffold_1G00043630 Rroxscaffold_2G00107640 Rroxscaffold_3G00273460 Rroxscaffold_5G00347960 Rroxscaffold_5G00350580 Rroxscaffold_5G00366890 Rroxscaffold_6G00391730 Rroxscaffold_6G00392580 Rroxscaffold_7G00170270 Rroxscaffold_7G00170280
rosa_rugosa Rorug02G0499800 Rorug04G0099000 Rorug04G0116000 Rorug05G0129300 Rorug05G0164200 Rorug06G0428300 Rorug06G0428400
rosa_samantha Rh1DG306200 Rh2AG348600 Rh2CG139500 Rh2CG334400 Rh2CG334500 Rh2CG334700 Rh2DG139800 Rh2DG373800 Rh5BG255900 Rh5CG289000 Rh5DG264300 Rh5DG449300 Rh6AG295400 Rh6BG299400 Rh6CG299300 Rh6DG291200 Rh7AG028200 Rh7BG027100 Rh7BG173900 Rh7CG029000 Rh7DG028500
rosa_wichuraiana Rw5G023500 Rw7G002240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 359
AccBSI CCGCTC 1 cut(s) 414
AccI GTMKAC 1 cut(s) 215
AccII CGCG 2 cut(s) 347, 434
AciI CCGC 8 cut(s) 237, 293, 317, 347, 387, 401, 414, 434
AclWI GGATC 1 cut(s) 424
AcoI YGGCCR 1 cut(s) 285
AcsI RAATTY 1 cut(s) 100
AcuI CTGAAG 1 cut(s) 233
AcyI GRCGYC 1 cut(s) 356
AgsI TTSAA 1 cut(s) 74
AjnI CCWGG 1 cut(s) 263
AluBI AGCT 3 cut(s) 12, 138, 178
AluI AGCT 3 cut(s) 12, 138, 178
AlwI GGATC 1 cut(s) 424
AoxI GGCC 1 cut(s) 285
ApeKI GCWGC 2 cut(s) 390, 452
ApoI RAATTY 1 cut(s) 100
AspS9I GGNCC 2 cut(s) 149, 267
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 1 cut(s) 95
AsuII TTCGAA 1 cut(s) 131
AvaII GGWCC 2 cut(s) 149, 267
BalI TGGCCA 1 cut(s) 287
BbvI GCAGC 1 cut(s) 377
BccI CCATC 1 cut(s) 101
BceAI ACGGC 2 cut(s) 234, 292
BciT130I CCWGG 1 cut(s) 265
BcnI CCSGG 1 cut(s) 162
BfaI CTAG 1 cut(s) 179
BglII AGATCT 1 cut(s) 22
BisI GCNGC 5 cut(s) 318, 388, 391, 435, 453
BlsI GCNGC 5 cut(s) 319, 389, 392, 436, 454
Bme1390I CCNGG 2 cut(s) 162, 265
Bme18I GGWCC 2 cut(s) 149, 267
BmgT120I GGNCC 2 cut(s) 149, 267
BmrFI CCNGG 2 cut(s) 162, 265
BmsI GCATC 2 cut(s) 22, 253
Bpu14I TTCGAA 1 cut(s) 131
BpuMI CCSGG 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 356
BsaJI CCNNGG 2 cut(s) 161, 288
Bse1I ACTGG 2 cut(s) 101, 142
BseBI CCWGG 1 cut(s) 265
BseDI CCNNGG 2 cut(s) 161, 288
BseGI GGATG 2 cut(s) 37, 112
BseMII CTCAG 1 cut(s) 401
BseNI ACTGG 2 cut(s) 101, 142
BseRI GAGGAG 2 cut(s) 22, 61
BseXI GCAGC 1 cut(s) 377
Bsh1236I CGCG 2 cut(s) 347, 434
BshFI GGCC 1 cut(s) 287
BsiSI CCGG 2 cut(s) 161, 301
BslFI GGGAC 1 cut(s) 31
BsmFI GGGAC 1 cut(s) 31
BsnI GGCC 1 cut(s) 287
Bsp119I TTCGAA 1 cut(s) 131
Bsp143I GATC 4 cut(s) 22, 76, 342, 416
Bsp19I CCATGG 1 cut(s) 288
BspACI CCGC 8 cut(s) 237, 293, 317, 347, 387, 401, 414, 434
BspANI GGCC 1 cut(s) 287
BspCNI CTCAG 1 cut(s) 402
BspFNI CGCG 2 cut(s) 347, 434
BspPI GGATC 1 cut(s) 424
BspQI GCTCTTC 1 cut(s) 128
BspT104I TTCGAA 1 cut(s) 131
BsrBI CCGCTC 1 cut(s) 414
BsrI ACTGG 2 cut(s) 101, 142
BssECI CCNNGG 2 cut(s) 161, 288
BssMI GATC 4 cut(s) 22, 76, 342, 416
BssNI GRCGYC 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 288
Bst2UI CCWGG 1 cut(s) 265
Bst6I CTCTTC 2 cut(s) 59, 128
BstACI GRCGYC 1 cut(s) 356
BstBI TTCGAA 1 cut(s) 131
BstDEI CTNAG 1 cut(s) 410
BstDSI CCRYGG 1 cut(s) 288
BstF5I GGATG 2 cut(s) 37, 112
BstFNI CGCG 2 cut(s) 347, 434
BstKTI GATC 4 cut(s) 25, 79, 345, 419
BstMBI GATC 4 cut(s) 22, 76, 342, 416
BstMWI GCNNNNNNNGC 2 cut(s) 164, 250
BstNI CCWGG 1 cut(s) 265
BstSCI CCNGG 2 cut(s) 160, 263
BstUI CGCG 2 cut(s) 347, 434
BstV1I GCAGC 1 cut(s) 377
BstX2I RGATCY 1 cut(s) 22
BstYI RGATCY 1 cut(s) 22
BsuRI GGCC 1 cut(s) 287
BtgI CCRYGG 1 cut(s) 288
BtsCI GGATG 2 cut(s) 37, 112
BtsIMutI CAGTG 1 cut(s) 426
Cfr13I GGNCC 2 cut(s) 149, 267
CseI GACGC 1 cut(s) 382
CviAII CATG 2 cut(s) 226, 289
CviJI RGCY 7 cut(s) 12, 111, 138, 178, 221, 287, 390
CviKI_1 RGCY 7 cut(s) 12, 111, 138, 178, 221, 287, 390
DdeI CTNAG 1 cut(s) 410
DpnI GATC 4 cut(s) 24, 78, 344, 418
DpnII GATC 4 cut(s) 22, 76, 342, 416
EaeI YGGCCR 1 cut(s) 285
Eam1104I CTCTTC 2 cut(s) 59, 128
EarI CTCTTC 2 cut(s) 59, 128
Eco130I CCWWGG 1 cut(s) 288
Eco47I GGWCC 2 cut(s) 149, 267
Eco57I CTGAAG 1 cut(s) 233
EcoRI GAATTC 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 263
EcoT14I CCWWGG 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 2 cut(s) 229, 292
FaiI YATR 4 cut(s) 227, 234, 290, 364
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 1 cut(s) 31
FatI CATG 2 cut(s) 225, 288
FauI CCCGC 1 cut(s) 340
FblI GTMKAC 1 cut(s) 215
Fnu4HI GCNGC 5 cut(s) 318, 388, 391, 435, 453
FokI GGATG 2 cut(s) 44, 119
Fsp4HI GCNGC 5 cut(s) 318, 388, 391, 435, 453
FspBI CTAG 1 cut(s) 179
GluI GCNGC 5 cut(s) 318, 388, 391, 435, 453
HaeIII GGCC 1 cut(s) 287
HapII CCGG 2 cut(s) 161, 301
HgaI GACGC 1 cut(s) 382
Hin1I GRCGYC 1 cut(s) 356
Hin1II CATG 2 cut(s) 229, 292
HincII GTYRAC 2 cut(s) 192, 216
HindII GTYRAC 2 cut(s) 192, 216
HinfI GANTC 2 cut(s) 258, 369
HpaII CCGG 2 cut(s) 161, 301
HphI GGTGA 1 cut(s) 95
Hpy166II GTNNAC 2 cut(s) 192, 216
Hpy188I TCNGA 3 cut(s) 106, 379, 411
Hpy188III TCNNGA 1 cut(s) 74
Hpy8I GTNNAC 2 cut(s) 192, 216
Hpy99I CGWCG 3 cut(s) 220, 358, 361
HpyAV CCTTC 2 cut(s) 115, 177
HpyCH4IV ACGT 2 cut(s) 356, 382
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 250
HpyF3I CTNAG 1 cut(s) 410
HpySE526I ACGT 2 cut(s) 356, 382
Hsp92I GRCGYC 1 cut(s) 356
Hsp92II CATG 2 cut(s) 229, 292
Kzo9I GATC 4 cut(s) 22, 76, 342, 416
LguI GCTCTTC 1 cut(s) 128
LmnI GCTCC 3 cut(s) 9, 48, 163
Lsp1109I GCAGC 1 cut(s) 377
LweI GCATC 2 cut(s) 22, 253
MaeI CTAG 1 cut(s) 179
MaeII ACGT 2 cut(s) 356, 382
MaeIII GTNAC 1 cut(s) 272
MalI GATC 4 cut(s) 24, 78, 344, 418
MbiI CCGCTC 1 cut(s) 414
MboI GATC 4 cut(s) 22, 76, 342, 416
MboII GAAGA 4 cut(s) 16, 76, 145, 239
MflI RGATCY 1 cut(s) 22
MlsI TGGCCA 1 cut(s) 287
MluCI AATT 1 cut(s) 100
MluNI TGGCCA 1 cut(s) 287
MnlI CCTC 4 cut(s) 39, 79, 315, 421
Mox20I TGGCCA 1 cut(s) 287
MscI TGGCCA 1 cut(s) 287
Msp20I TGGCCA 1 cut(s) 287
MspI CCGG 2 cut(s) 161, 301
MspR9I CCNGG 2 cut(s) 162, 265
MvaI CCWGG 1 cut(s) 265
MvnI CGCG 2 cut(s) 347, 434
MwoI GCNNNNNNNGC 2 cut(s) 164, 250
NciI CCSGG 1 cut(s) 162
NcoI CCATGG 1 cut(s) 288
NdeII GATC 4 cut(s) 22, 76, 342, 416
NlaIII CATG 2 cut(s) 229, 292
NmuCI GTSAC 1 cut(s) 272
NspV TTCGAA 1 cut(s) 131
PciSI GCTCTTC 1 cut(s) 128
PcsI WCGNNNNNNNCGW 1 cut(s) 438
PfeI GAWTC 2 cut(s) 258, 369
PfoI TCCNGGA 1 cut(s) 263
PkrI GCNGC 5 cut(s) 319, 389, 392, 436, 454
Psp6I CCWGG 1 cut(s) 263
PspGI CCWGG 1 cut(s) 263
PspPI GGNCC 2 cut(s) 149, 267
PsuI RGATCY 1 cut(s) 22
SalI GTCGAC 1 cut(s) 214
SapI GCTCTTC 1 cut(s) 128
SatI GCNGC 5 cut(s) 318, 388, 391, 435, 453
Sau3AI GATC 4 cut(s) 22, 76, 342, 416
Sau96I GGNCC 2 cut(s) 149, 267
ScrFI CCNGG 2 cut(s) 162, 265
SfaNI GCATC 2 cut(s) 22, 253
SfuI TTCGAA 1 cut(s) 131
SinI GGWCC 2 cut(s) 149, 267
Sse9I AATT 1 cut(s) 100
SsiI CCGC 8 cut(s) 237, 293, 317, 347, 387, 401, 414, 434
SspMI CTAG 1 cut(s) 179
StyD4I CCNGG 2 cut(s) 160, 263
StyI CCWWGG 1 cut(s) 288
TaiI ACGT 2 cut(s) 359, 385
TaqI TCGA 2 cut(s) 131, 215
TasI AATT 1 cut(s) 100
TauI GCSGC 3 cut(s) 320, 390, 437
TfiI GAWTC 2 cut(s) 258, 369
TscAI CASTG 1 cut(s) 426
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 2 cut(s) 390, 452
Tsp45I GTSAC 1 cut(s) 272
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 426
VpaK11BI GGWCC 2 cut(s) 149, 267
XapI RAATTY 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 215
XspI CTAG 1 cut(s) 179
ZraI GACGTC 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.