Rroxscaffold_5G00347960

Translation factor GUF1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000573
Physical Location & Seq
Forward (+)
19776587 .. 19777267
681 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00347960.1

Sequence Viewer

Length: 597 bp
ATGCCCGGACATGCAGACCGTGGTGGTGAAGTGGAGCTGGTTGTTGGTATGGTGGAGGGAACAGTTTTGGTTGTTGATGCGGGGAAGGTTCGCTTGCACGGACCAAGTTGTGCTTGCCAAAGCCTTAAGATCGGCTGCGCGTCCTATCCTCCTTCTAAAAAAGTTGATCAGCCTGCAGTTTTGAAGAGATGTGCGATGAAGATAAGAGTCGAGTATTTGATCCTTTCGCAAATCTCGGTGCTACGGAGGAGCAACTGGATTTTCCGTGCTATGTGCTTCGCTAAAGAAGAGATGCAAGGTTGTGGAGAAGGGAGTTCAAGATCGGGTGAGGTTGGGAACTGCAATTCGAATGGGCGTGGGGCGAAGGAGAGTTCGAAGAGCGGCCAGCTTGGTCCATTCGCTCCGGTGGCAGTTCCAAGAACTAAGAAAGGTGTTGACATCAACAAGGTTGTTGGGAGAGTCTTGGACTCTTGGGGCTTATTATTGGCCTCGTATCGAATCGCCAAGGGAAAAAGAAGAGTTCGGGAACTTCATGCCACAAGCCATTACGTGCCTGGTCCCGAAGTTGACCGATTGGCTTCAATGGATGGAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2

Protein Analysis

198

Amino Acids

21.29

Weight (kDa)

9.68

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000573)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48160 AT3G48160
fragaria_vesca FvH4_3g21790 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330
malus_domestica MD06G1181700.v1.1 MD14G1187600.v1.1
prunus_persica Prupe.5G180000_v2.0.a1 Prupe.5G180000_v2.0.a1 Prupe.5G180000_v2.0.a1 Prupe.5G180000_v2.0.a1
pyrus_communis pycom06g16070 pycom14g15550
rosa_chinensis RchiOBHm_Chr2g0100091 RchiOBHm_Chr2g0131211 RchiOBHm_Chr2g0131231 RchiOBHm_Chr2g0155761 RchiOBHm_Chr5g0037341 RchiOBHm_Chr6g0286771 RchiOBHm_Chr7g0179801
rosa_laevigata RLG00000005323 RLG00000033775
rosa_multiflora Rmu_co8405049.1_g000002 Rmu_sc0003547.1_g000004 Rmu_sc0004156.1_g000008 Rmu_sc0007546.1_g000012 Rmu_sc0026307.1_g000001 Rmu_sc0032585.1_g000001 Rmu_ssc0000019.1_g000022
rosa_roxburghii Rroxscaffold_1G00027620 Rroxscaffold_1G00027630 Rroxscaffold_1G00043630 Rroxscaffold_2G00107640 Rroxscaffold_3G00273460 Rroxscaffold_5G00347960 Rroxscaffold_5G00350580 Rroxscaffold_5G00366890 Rroxscaffold_6G00391730 Rroxscaffold_6G00392580 Rroxscaffold_7G00170270 Rroxscaffold_7G00170280
rosa_rugosa Rorug02G0499800 Rorug04G0099000 Rorug04G0116000 Rorug05G0129300 Rorug05G0164200 Rorug06G0428300 Rorug06G0428400
rosa_samantha Rh1DG306200 Rh2AG348600 Rh2CG139500 Rh2CG334400 Rh2CG334500 Rh2CG334700 Rh2DG139800 Rh2DG373800 Rh5BG255900 Rh5CG289000 Rh5DG264300 Rh5DG449300 Rh6AG295400 Rh6BG299400 Rh6CG299300 Rh6DG291200 Rh7AG028200 Rh7BG027100 Rh7BG173900 Rh7CG029000 Rh7DG028500
rosa_wichuraiana Rw5G023500 Rw7G002240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 381
AccII CGCG 1 cut(s) 140
AciI CCGC 2 cut(s) 80, 381
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 1 cut(s) 382
AflII CTTAAG 1 cut(s) 125
AgsI TTSAA 3 cut(s) 184, 318, 582
AjnI CCWGG 1 cut(s) 553
AluBI AGCT 2 cut(s) 37, 388
AluI AGCT 2 cut(s) 37, 388
AlwI GGATC 1 cut(s) 214
AoxI GGCC 2 cut(s) 382, 486
ApeKI GCWGC 1 cut(s) 135
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 3 cut(s) 101, 392, 557
AsuC2I CCSGG 1 cut(s) 6
AsuHPI GGTGA 2 cut(s) 38, 338
AsuII TTCGAA 2 cut(s) 347, 374
AvaII GGWCC 3 cut(s) 101, 392, 557
BbvI GCAGC 1 cut(s) 122
BccI CCATC 1 cut(s) 581
BciT130I CCWGG 1 cut(s) 555
BclI TGATCA 1 cut(s) 166
BcnI CCSGG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 174
BfrI CTTAAG 1 cut(s) 125
BisI GCNGC 2 cut(s) 136, 382
BlsI GCNGC 2 cut(s) 137, 383
Bme1390I CCNGG 2 cut(s) 6, 555
Bme18I GGWCC 3 cut(s) 101, 392, 557
BmgT120I GGNCC 3 cut(s) 101, 392, 557
BmiI GGNNCC 1 cut(s) 559
BmrFI CCNGG 2 cut(s) 6, 555
BmsI GCATC 2 cut(s) 67, 282
Bpu14I TTCGAA 2 cut(s) 347, 374
BpuMI CCSGG 1 cut(s) 6
BsaAI YACGTR 1 cut(s) 550
BsaJI CCNNGG 2 cut(s) 19, 504
BsaWI WCCGGW 1 cut(s) 403
Bse1I ACTGG 1 cut(s) 260
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 19, 504
BseGI GGATG 1 cut(s) 592
BseNI ACTGG 1 cut(s) 260
BseRI GAGGAG 1 cut(s) 262
BseXI GCAGC 1 cut(s) 122
Bsh1236I CGCG 1 cut(s) 140
BshFI GGCC 2 cut(s) 384, 488
BsiSI CCGG 2 cut(s) 6, 404
BslFI GGGAC 1 cut(s) 543
BsmFI GGGAC 1 cut(s) 543
BsnI GGCC 2 cut(s) 384, 488
Bsp119I TTCGAA 2 cut(s) 347, 374
Bsp143I GATC 4 cut(s) 129, 166, 219, 320
BspACI CCGC 2 cut(s) 80, 381
BspANI GGCC 2 cut(s) 384, 488
BspFNI CGCG 1 cut(s) 140
BspLI GGNNCC 1 cut(s) 559
BspMAI CTGCAG 1 cut(s) 178
BspPI GGATC 1 cut(s) 214
BspQI GCTCTTC 1 cut(s) 371
BspT104I TTCGAA 2 cut(s) 347, 374
BspTI CTTAAG 1 cut(s) 125
BsrBI CCGCTC 1 cut(s) 381
BsrI ACTGG 1 cut(s) 260
BssECI CCNNGG 2 cut(s) 19, 504
BssMI GATC 4 cut(s) 129, 166, 219, 320
BssT1I CCWWGG 1 cut(s) 504
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 2 cut(s) 20, 64
Bst6I CTCTTC 4 cut(s) 179, 282, 371, 511
BstAFI CTTAAG 1 cut(s) 125
BstBAI YACGTR 1 cut(s) 550
BstBI TTCGAA 2 cut(s) 347, 374
BstC8I GCNNGC 4 cut(s) 95, 115, 174, 386
BstDEI CTNAG 1 cut(s) 423
BstDSI CCRYGG 1 cut(s) 19
BstF5I GGATG 1 cut(s) 592
BstFNI CGCG 1 cut(s) 140
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 4 cut(s) 132, 169, 222, 323
BstMBI GATC 4 cut(s) 129, 166, 219, 320
BstMWI GCNNNNNNNGC 1 cut(s) 407
BstNI CCWGG 1 cut(s) 555
BstNSI RCATGY 1 cut(s) 14
BstSCI CCNGG 2 cut(s) 4, 553
BstSFI CTRYAG 1 cut(s) 174
BstUI CGCG 1 cut(s) 140
BstV1I GCAGC 1 cut(s) 122
BsuRI GGCC 2 cut(s) 384, 488
BtgI CCRYGG 1 cut(s) 19
BtgZI GCGATG 1 cut(s) 209
BtsCI GGATG 1 cut(s) 592
Cac8I GCNNGC 4 cut(s) 95, 115, 174, 386
CfoI GCGC 1 cut(s) 140
Cfr13I GGNCC 3 cut(s) 101, 392, 557
CseI GACGC 1 cut(s) 129
CviAII CATG 2 cut(s) 11, 533
DdeI CTNAG 1 cut(s) 423
DpnI GATC 4 cut(s) 131, 168, 221, 322
DpnII GATC 4 cut(s) 129, 166, 219, 320
EaeI YGGCCR 1 cut(s) 382
Eam1104I CTCTTC 4 cut(s) 179, 282, 371, 511
EarI CTCTTC 4 cut(s) 179, 282, 371, 511
Eco130I CCWWGG 1 cut(s) 504
Eco47I GGWCC 3 cut(s) 101, 392, 557
EcoRII CCWGG 1 cut(s) 553
EcoT14I CCWWGG 1 cut(s) 504
ErhI CCWWGG 1 cut(s) 504
FaeI CATG 2 cut(s) 14, 536
FaiI YATR 4 cut(s) 12, 50, 272, 534
FalI AAGNNNNNCTT 4 cut(s) 77, 109, 97, 129
FaqI GGGAC 1 cut(s) 543
FatI CATG 2 cut(s) 10, 532
FauI CCCGC 1 cut(s) 73
FbaI TGATCA 1 cut(s) 166
Fnu4HI GCNGC 2 cut(s) 136, 382
Fsp4HI GCNGC 2 cut(s) 136, 382
GlaI GCGC 1 cut(s) 139
GluI GCNGC 2 cut(s) 136, 382
HaeIII GGCC 2 cut(s) 384, 488
HapII CCGG 2 cut(s) 6, 404
HgaI GACGC 1 cut(s) 129
HhaI GCGC 1 cut(s) 140
Hin1II CATG 2 cut(s) 14, 536
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HincII GTYRAC 2 cut(s) 436, 568
HindII GTYRAC 2 cut(s) 436, 568
HinfI GANTC 4 cut(s) 207, 459, 467, 498
HpaII CCGG 2 cut(s) 6, 404
HphI GGTGA 2 cut(s) 38, 338
Hpy166II GTNNAC 2 cut(s) 436, 568
Hpy188III TCNNGA 3 cut(s) 318, 524, 560
Hpy8I GTNNAC 2 cut(s) 436, 568
HpyAV CCTTC 4 cut(s) 79, 162, 302, 358
HpyCH4III ACNGT 2 cut(s) 20, 64
HpyCH4IV ACGT 1 cut(s) 549
HpyCH4V TGCA 5 cut(s) 14, 97, 176, 295, 342
HpyF10VI GCNNNNNNNGC 1 cut(s) 407
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 1 cut(s) 549
Hsp92II CATG 2 cut(s) 14, 536
HspAI GCGC 1 cut(s) 138
Ksp22I TGATCA 1 cut(s) 166
Kzo9I GATC 4 cut(s) 129, 166, 219, 320
LguI GCTCTTC 1 cut(s) 371
LmnI GCTCC 3 cut(s) 34, 249, 406
LpnPI CCDG 8 cut(s) 19, 23, 186, 241, 398, 417, 540, 567
Lsp1109I GCAGC 1 cut(s) 122
LweI GCATC 2 cut(s) 67, 282
MaeII ACGT 1 cut(s) 549
MalI GATC 4 cut(s) 131, 168, 221, 322
MbiI CCGCTC 1 cut(s) 381
MboI GATC 4 cut(s) 129, 166, 219, 320
MboII GAAGA 5 cut(s) 196, 211, 299, 388, 528
MluCI AATT 1 cut(s) 343
MlyI GAGTC 3 cut(s) 216, 461, 468
MnlI CCTC 5 cut(s) 49, 159, 240, 322, 499
MseI TTAA 1 cut(s) 126
MspCI CTTAAG 1 cut(s) 125
MspI CCGG 2 cut(s) 6, 404
MspR9I CCNGG 2 cut(s) 6, 555
MvaI CCWGG 1 cut(s) 555
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 1 cut(s) 407
NciI CCSGG 1 cut(s) 6
NdeII GATC 4 cut(s) 129, 166, 219, 320
NlaIII CATG 2 cut(s) 14, 536
NlaIV GGNNCC 1 cut(s) 559
NspI RCATGY 1 cut(s) 14
NspV TTCGAA 2 cut(s) 347, 374
PciSI GCTCTTC 1 cut(s) 371
PfeI GAWTC 1 cut(s) 498
PkrI GCNGC 2 cut(s) 137, 383
PleI GAGTC 3 cut(s) 215, 461, 467
PpsI GAGTC 3 cut(s) 215, 461, 467
Ppu21I YACGTR 1 cut(s) 550
Psp6I CCWGG 1 cut(s) 553
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 1 cut(s) 559
PspPI GGNCC 3 cut(s) 101, 392, 557
PstI CTGCAG 1 cut(s) 178
SapI GCTCTTC 1 cut(s) 371
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 2 cut(s) 136, 382
Sau3AI GATC 4 cut(s) 129, 166, 219, 320
Sau96I GGNCC 3 cut(s) 101, 392, 557
SchI GAGTC 3 cut(s) 216, 461, 468
ScrFI CCNGG 2 cut(s) 6, 555
SetI ASST 8 cut(s) 39, 90, 301, 333, 390, 433, 450, 552
SfaNI GCATC 2 cut(s) 67, 282
SfcI CTRYAG 1 cut(s) 174
SfuI TTCGAA 2 cut(s) 347, 374
SinI GGWCC 3 cut(s) 101, 392, 557
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 1 cut(s) 343
SsiI CCGC 2 cut(s) 80, 381
StyD4I CCNGG 2 cut(s) 4, 553
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 2 cut(s) 20, 64
TaiI ACGT 1 cut(s) 552
TaqI TCGA 4 cut(s) 210, 347, 374, 496
TaqII GACCGA 1 cut(s) 585
TasI AATT 1 cut(s) 343
TauI GCSGC 1 cut(s) 384
TfiI GAWTC 1 cut(s) 498
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TseI GCWGC 1 cut(s) 135
TspDTI ATGAA 2 cut(s) 212, 521
TspGWI ACGGA 3 cut(s) 114, 254, 259
Vha464I CTTAAG 1 cut(s) 125
VpaK11BI GGWCC 3 cut(s) 101, 392, 557
XceI RCATGY 1 cut(s) 14
XcmI CCANNNNNNNNNTGG 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.