Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000573

Physical Location & Seq

Genomic Coordinates Reverse (-)

27972747 .. 27975569

Gene Structure 2823 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1187600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1161 bp

ATGACTGCTCCTCCATCCCCACCTCCAGCCACAGAGCAACAACCGGACCCAGCTTCAACAGGGCCTTCCGCCAGAAACCACGGCTACAGCAGAAAGCAGAAGTCTCTAGGCCTCTTGTGCTCCAATTTCCTCGTCCTCTACAACCGCGACGGCGTGACTTCAATCGGCCTTGACGACGCCGCTTCACGACTTGGTGTTGAGAGGCGGAGGATCTACGATATTGTCAACGTCCTGGAGAGCGTTGGGGTTCTTGCTCGAAAGGCGAAGAACCAGTATTCTTGGAAAGGATTTAAGGCGATCCCTAATGCCCTGCAAGAGCTAAGGGAAGAGGGCCTGAGAGAGAATATTTGTAACTTTGACGGCAACGAAGATCCAAAGGGTTATCAAATTTCAGACGACGAGGATGATGCAGAAAGATGTGGGAGTCAGCAGAATGAAAACTCCAACCCTACTCTCAATGTTAAACCTATGAACCCAAAATCAGATAATAGAAGGGAAAAGTCGCTGGCGCTTCTTACGCAGAATTTTGTGAAGCTCTTTGTGTGCTCAACTGTTGAAACGATTTCCCTTGATGAAGCTGCCAAATCACTGCTTGGAGATGCACACAAAGCATCAGTGATGAGAAGTAGTAAATTTAAACTCTTGACTAACTTTTGTTCGAAACTTGATGAAGCAGCAAAGGTAAGGCGGATTTATGATATTGCGAATGTTTTGTCATCCATGAACCTCATTGAGAAGACCCACACATCAGATACGAGGAAGCCTGCATTTAAGTGGTTGGGATTAAGAGGAAAAGAGGAGGTTCCTCAGGAGACTAAGAAGAGAGCTTTTGGCACAGATATAACGAACGTCAGTTCTAAGAGGGGAAAGGTAGACTCTTTTATTGGTGGGAAGTTGGATGGGCAAAAACAGAAGGGCCTAGTAGGTGAGGCTGATAGGAGCAATTTGGAAGACTCAAAAGATGGTTCAAAGAGCTATCAGTTTGGTCCTTTTGCTCCTGTCACTATTGCCAGAGCTGGGACTGGCAACACGAGGAACGTTCATGACTGGGAGAAATTGACGTCTACTTATCGTCCTCAGTATCAAAACCAAGGACCAGCTGACAATAGTGGGAGAACGAGAAGGGAGGTGGATAGAGAAAGAAACATGACCGGAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006355 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0019219 GO:0019222 GO:0022402 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032875 GO:0032876 GO:0034641 GO:0034645 GO:0042023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0045786 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051252 GO:0051726 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090329 GO:0097159 GO:0140110 GO:1901360 GO:1901363 GO:1901576 GO:1903506 GO:2000104 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E2F_TDP

Protein Analysis

387

Amino Acids

42.85

Weight (kDa)

9.16

Isoelectric Point (pI)

35.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_E2F_TDP	PF02319	32 - 96	1.1e-21	E2F/DP family winged-helix DNA-binding domain
WHD_E2F_TDP	PF02319	165 - 261	6e-19	E2F/DP family winged-helix DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000573)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48160 AT3G48160
fragaria_vesca	FvH4_3g21790 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330 FvH4_5g16330
malus_domestica	MD06G1181700.v1.1 MD14G1187600.v1.1
prunus_persica	Prupe.5G180000_v2.0.a1 Prupe.5G180000_v2.0.a1 Prupe.5G180000_v2.0.a1 Prupe.5G180000_v2.0.a1
pyrus_communis	pycom06g16070 pycom14g15550
rosa_chinensis	RchiOBHm_Chr2g0100091 RchiOBHm_Chr2g0131211 RchiOBHm_Chr2g0131231 RchiOBHm_Chr2g0155761 RchiOBHm_Chr5g0037341 RchiOBHm_Chr6g0286771 RchiOBHm_Chr7g0179801
rosa_laevigata	RLG00000005323 RLG00000033775
rosa_multiflora	Rmu_co8405049.1_g000002 Rmu_sc0003547.1_g000004 Rmu_sc0004156.1_g000008 Rmu_sc0007546.1_g000012 Rmu_sc0026307.1_g000001 Rmu_sc0032585.1_g000001 Rmu_ssc0000019.1_g000022
rosa_roxburghii	Rroxscaffold_1G00027620 Rroxscaffold_1G00027630 Rroxscaffold_1G00043630 Rroxscaffold_2G00107640 Rroxscaffold_3G00273460 Rroxscaffold_5G00347960 Rroxscaffold_5G00350580 Rroxscaffold_5G00366890 Rroxscaffold_6G00391730 Rroxscaffold_6G00392580 Rroxscaffold_7G00170270 Rroxscaffold_7G00170280
rosa_rugosa	Rorug02G0499800 Rorug04G0099000 Rorug04G0116000 Rorug05G0129300 Rorug05G0164200 Rorug06G0428300 Rorug06G0428400
rosa_samantha	Rh1DG306200 Rh2AG348600 Rh2CG139500 Rh2CG334400 Rh2CG334500 Rh2CG334700 Rh2DG139800 Rh2DG373800 Rh5BG255900 Rh5CG289000 Rh5DG264300 Rh5DG449300 Rh6AG295400 Rh6BG299400 Rh6CG299300 Rh6DG291200 Rh7AG028200 Rh7BG027100 Rh7BG173900 Rh7CG029000 Rh7DG028500
rosa_wichuraiana	Rw5G023500 Rw7G002240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1064
AccI	GTMKAC	2 cut(s)	873, 1064
AccII	CGCG	1 cut(s)	147
AciI	CCGC	5 cut(s)	69, 145, 180, 205, 688
AclI	AACGTT	1 cut(s)	1038
AclWI	GGATC	3 cut(s)	218, 292, 365
AcsI	RAATTY	3 cut(s)	387, 523, 632
AcyI	GRCGYC	2 cut(s)	177, 1061
AfiI	CCNNNNNNNGG	1 cut(s)	1017
AgsI	TTSAA	4 cut(s)	57, 162, 557, 969
AjnI	CCWGG	1 cut(s)	231
AluBI	AGCT	8 cut(s)	53, 319, 535, 578, 827, 975, 1016, 1100
AluI	AGCT	8 cut(s)	53, 319, 535, 578, 827, 975, 1016, 1100
Alw21I	GWGCWC	2 cut(s)	122, 548
Alw26I	GTCTC	3 cut(s)	108, 806, 1149
AlwI	GGATC	3 cut(s)	218, 292, 365
AoxI	GGCC	5 cut(s)	62, 109, 166, 331, 916
ApeKI	GCWGC	2 cut(s)	578, 674
ApoI	RAATTY	3 cut(s)	387, 523, 632
ArsI	GACNNNNNNTTYG	2 cut(s)	698, 730
AspLEI	GCGC	1 cut(s)	511
AspS9I	GGNCC	6 cut(s)	46, 62, 331, 916, 986, 1094
AsuHPI	GGTGA	1 cut(s)	938
AsuII	TTCGAA	1 cut(s)	659
AvaII	GGWCC	3 cut(s)	46, 986, 1094
AxyI	CCTNAGG	1 cut(s)	807
BauI	CACGAG	1 cut(s)	1030
BbsI	GAAGAC	2 cut(s)	743, 957
Bbv12I	GWGCWC	2 cut(s)	122, 548
BbvI	GCAGC	2 cut(s)	565, 686
BccI	CCATC	3 cut(s)	22, 893, 956
BceAI	ACGGC	3 cut(s)	97, 166, 376
BcgI	CGANNNNNNTGC	2 cut(s)	389, 423
BciT130I	CCWGG	1 cut(s)	233
BcoDI	GTCTC	3 cut(s)	108, 806, 1149
BfaI	CTAG	2 cut(s)	107, 920
BfmI	CTRYAG	1 cut(s)	85
BfoI	RGCGCY	1 cut(s)	512
BisI	GCNGC	3 cut(s)	180, 579, 675
BlsI	GCNGC	3 cut(s)	181, 580, 676
Bme1390I	CCNGG	1 cut(s)	233
Bme18I	GGWCC	3 cut(s)	46, 986, 1094
BmgT120I	GGNCC	6 cut(s)	46, 62, 331, 916, 986, 1094
BmiI	GGNNCC	2 cut(s)	48, 804
BmrFI	CCNGG	1 cut(s)	233
BmrI	ACTGGG	1 cut(s)	1057
BmsI	GCATC	3 cut(s)	397, 589, 620
BmuI	ACTGGG	1 cut(s)	1057
BpiI	GAAGAC	2 cut(s)	743, 957
BpmI	CTGGAG	2 cut(s)	9, 254
Bpu10I	CCTNAGC	1 cut(s)	320
Bpu14I	TTCGAA	1 cut(s)	659
BsaHI	GRCGYC	2 cut(s)	177, 1061
BsaJI	CCNNGG	2 cut(s)	79, 1090
BsaWI	WCCGGW	2 cut(s)	43, 1151
BsaXI	ACNNNNNCTCC	1 cut(s)	25
Bsc4I	CCNNNNNNNGG	1 cut(s)	1017
Bse1I	ACTGG	3 cut(s)	271, 1027, 1052
Bse21I	CCTNAGG	1 cut(s)	807
BseBI	CCWGG	1 cut(s)	233
BseDI	CCNNGG	2 cut(s)	79, 1090
BseGI	GGATG	4 cut(s)	14, 409, 716, 904
BseLI	CCNNNNNNNGG	1 cut(s)	1017
BseMII	CTCAG	3 cut(s)	326, 821, 1091
BseNI	ACTGG	3 cut(s)	271, 1027, 1052
BseRI	GAGGAG	1 cut(s)	812
BseXI	GCAGC	2 cut(s)	565, 686
BseYI	CCCAGC	2 cut(s)	49, 1016
Bsh1236I	CGCG	1 cut(s)	147
BshFI	GGCC	5 cut(s)	64, 111, 168, 333, 918
BsiHKAI	GWGCWC	2 cut(s)	122, 548
BsiSI	CCGG	2 cut(s)	44, 1152
BslFI	GGGAC	1 cut(s)	1033
BslI	CCNNNNNNNGG	1 cut(s)	1017
BsmAI	GTCTC	3 cut(s)	108, 806, 1149
BsmFI	GGGAC	1 cut(s)	1033
BsnI	GGCC	5 cut(s)	64, 111, 168, 333, 918
Bsp119I	TTCGAA	1 cut(s)	659
Bsp1286I	GDGCHC	2 cut(s)	122, 548
Bsp143I	GATC	3 cut(s)	210, 297, 370
BspACI	CCGC	5 cut(s)	69, 145, 180, 205, 688
BspANI	GGCC	5 cut(s)	64, 111, 168, 333, 918
BspCNI	CTCAG	3 cut(s)	327, 820, 1090
BspFNI	CGCG	1 cut(s)	147
BspHI	TCATGA	1 cut(s)	1042
BspLI	GGNNCC	2 cut(s)	48, 804
BspPI	GGATC	3 cut(s)	218, 292, 365
BspT104I	TTCGAA	1 cut(s)	659
BsrI	ACTGG	3 cut(s)	271, 1027, 1052
BssECI	CCNNGG	2 cut(s)	79, 1090
BssMI	GATC	3 cut(s)	210, 297, 370
BssNI	GRCGYC	2 cut(s)	177, 1061
BssSI	CACGAG	1 cut(s)	1030
BssT1I	CCWWGG	1 cut(s)	1090
Bst2BI	CACGAG	1 cut(s)	1030
Bst2UI	CCWGG	1 cut(s)	233
Bst4CI	ACNGT	1 cut(s)	553
Bst6I	CTCTTC	2 cut(s)	321, 815
BstACI	GRCGYC	2 cut(s)	177, 1061
BstBI	TTCGAA	1 cut(s)	659
BstC8I	GCNNGC	2 cut(s)	507, 765
BstDEI	CTNAG	6 cut(s)	320, 335, 807, 816, 858, 1077
BstDSI	CCRYGG	1 cut(s)	79
BstF5I	GGATG	4 cut(s)	14, 409, 716, 904
BstFNI	CGCG	1 cut(s)	147
BstH2I	RGCGCY	1 cut(s)	512
BstHHI	GCGC	1 cut(s)	511
BstKTI	GATC	3 cut(s)	213, 300, 373
BstMAI	GTCTC	3 cut(s)	108, 806, 1149
BstMBI	GATC	3 cut(s)	210, 297, 370
BstMWI	GCNNNNNNNGC	4 cut(s)	117, 260, 517, 608
BstNI	CCWGG	1 cut(s)	233
BstSCI	CCNGG	1 cut(s)	231
BstSFI	CTRYAG	1 cut(s)	85
BstUI	CGCG	1 cut(s)	147
BstV1I	GCAGC	2 cut(s)	565, 686
BstV2I	GAAGAC	2 cut(s)	743, 957
BstX2I	RGATCY	2 cut(s)	210, 370
BstYI	RGATCY	2 cut(s)	210, 370
Bsu36I	CCTNAGG	1 cut(s)	807
BsuRI	GGCC	5 cut(s)	64, 111, 168, 333, 918
BtgI	CCRYGG	1 cut(s)	79
BtsCI	GGATG	4 cut(s)	14, 409, 716, 904
BtsI	GCAGTG	1 cut(s)	587
BtsIMutI	CAGTG	2 cut(s)	587, 621
Cac8I	GCNNGC	2 cut(s)	507, 765
CciI	TCATGA	1 cut(s)	1042
CfoI	GCGC	1 cut(s)	511
Cfr13I	GGNCC	6 cut(s)	46, 62, 331, 916, 986, 1094
CseI	GACGC	1 cut(s)	185
CviAII	CATG	3 cut(s)	721, 1043, 1147
DdeI	CTNAG	6 cut(s)	320, 335, 807, 816, 858, 1077
DpnI	GATC	3 cut(s)	212, 299, 372
DpnII	GATC	3 cut(s)	210, 297, 370
DraI	TTTAAA	1 cut(s)	637
Eam1104I	CTCTTC	2 cut(s)	321, 815
EarI	CTCTTC	2 cut(s)	321, 815
EciI	GGCGGA	3 cut(s)	58, 220, 703
Eco130I	CCWWGG	1 cut(s)	1090
Eco147I	AGGCCT	1 cut(s)	111
Eco47I	GGWCC	3 cut(s)	46, 986, 1094
Eco81I	CCTNAGG	1 cut(s)	807
EcoO109I	RGGNCCY	3 cut(s)	62, 331, 916
EcoRII	CCWGG	1 cut(s)	231
EcoT14I	CCWWGG	1 cut(s)	1090
ErhI	CCWWGG	1 cut(s)	1090
FaeI	CATG	3 cut(s)	724, 1046, 1150
FaiI	YATR	6 cut(s)	470, 696, 722, 842, 1044, 1148
FaqI	GGGAC	1 cut(s)	1033
FatI	CATG	3 cut(s)	720, 1042, 1146
FblI	GTMKAC	2 cut(s)	873, 1064
Fnu4HI	GCNGC	3 cut(s)	180, 579, 675
FokI	GGATG	3 cut(s)	416, 703, 911
Fsp4HI	GCNGC	3 cut(s)	180, 579, 675
FspBI	CTAG	2 cut(s)	107, 920
GlaI	GCGC	1 cut(s)	510
GluI	GCNGC	3 cut(s)	180, 579, 675
GsaI	CCCAGC	2 cut(s)	53, 1020
GsuI	CTGGAG	2 cut(s)	9, 254
HaeII	RGCGCY	1 cut(s)	512
HaeIII	GGCC	5 cut(s)	64, 111, 168, 333, 918
HapII	CCGG	2 cut(s)	44, 1152
HgaI	GACGC	1 cut(s)	185
HhaI	GCGC	1 cut(s)	511
Hin1I	GRCGYC	2 cut(s)	177, 1061
Hin1II	CATG	3 cut(s)	724, 1046, 1150
Hin6I	GCGC	1 cut(s)	509
HinP1I	GCGC	1 cut(s)	509
HincII	GTYRAC	1 cut(s)	226
HindII	GTYRAC	1 cut(s)	226
HinfI	GANTC	3 cut(s)	424, 875, 953
HpaII	CCGG	2 cut(s)	44, 1152
HphI	GGTGA	1 cut(s)	938
Hpy166II	GTNNAC	3 cut(s)	226, 874, 1065
Hpy188I	TCNGA	3 cut(s)	394, 484, 751
Hpy188III	TCNNGA	4 cut(s)	186, 643, 809, 1043
Hpy8I	GTNNAC	3 cut(s)	226, 874, 1065
Hpy99I	CGWCG	3 cut(s)	152, 179, 401
HpyAV	CCTTC	4 cut(s)	75, 486, 907, 1116
HpyCH4III	ACNGT	1 cut(s)	553
HpyCH4IV	ACGT	4 cut(s)	228, 849, 1038, 1061
HpyCH4V	TGCA	4 cut(s)	313, 410, 602, 767
HpyF10VI	GCNNNNNNNGC	4 cut(s)	117, 260, 517, 608
HpyF3I	CTNAG	6 cut(s)	320, 335, 807, 816, 858, 1077
HpySE526I	ACGT	4 cut(s)	228, 849, 1038, 1061
Hsp92I	GRCGYC	2 cut(s)	177, 1061
Hsp92II	CATG	3 cut(s)	724, 1046, 1150
HspAI	GCGC	1 cut(s)	509
Kzo9I	GATC	3 cut(s)	210, 297, 370
LmnI	GCTCC	4 cut(s)	13, 125, 939, 1000
Lsp1109I	GCAGC	2 cut(s)	565, 686
LweI	GCATC	3 cut(s)	397, 589, 620
MaeI	CTAG	2 cut(s)	107, 920
MaeII	ACGT	4 cut(s)	228, 849, 1038, 1061
MaeIII	GTNAC	3 cut(s)	154, 350, 1000
MalI	GATC	3 cut(s)	212, 299, 372
MboI	GATC	3 cut(s)	210, 297, 370
MboII	GAAGA	6 cut(s)	277, 338, 380, 748, 832, 962
MflI	RGATCY	2 cut(s)	210, 370
MhlI	GDGCHC	2 cut(s)	122, 548
MluCI	AATT	6 cut(s)	124, 387, 523, 632, 943, 1055
MlyI	GAGTC	3 cut(s)	433, 869, 947
MmeI	TCCRAC	2 cut(s)	468, 876
MseI	TTAA	5 cut(s)	291, 462, 636, 771, 785
MslI	CAYNNNNRTG	1 cut(s)	772
MspA1I	CMGCKG	1 cut(s)	1100
MspI	CCGG	2 cut(s)	44, 1152
MspR9I	CCNGG	1 cut(s)	233
MvaI	CCWGG	1 cut(s)	233
MvnI	CGCG	1 cut(s)	147
MwoI	GCNNNNNNNGC	4 cut(s)	117, 260, 517, 608
NdeII	GATC	3 cut(s)	210, 297, 370
NlaIII	CATG	3 cut(s)	724, 1046, 1150
NlaIV	GGNNCC	2 cut(s)	48, 804
NmuCI	GTSAC	2 cut(s)	154, 1000
NspV	TTCGAA	1 cut(s)	659
PagI	TCATGA	1 cut(s)	1042
PceI	AGGCCT	1 cut(s)	111
PcsI	WCGNNNNNNNCGW	1 cut(s)	171
PfoI	TCCNGGA	1 cut(s)	231
PkrI	GCNGC	3 cut(s)	181, 580, 676
PleI	GAGTC	3 cut(s)	432, 869, 947
PpsI	GAGTC	3 cut(s)	432, 869, 947
Psp1406I	AACGTT	1 cut(s)	1038
Psp6I	CCWGG	1 cut(s)	231
PspFI	CCCAGC	2 cut(s)	49, 1016
PspGI	CCWGG	1 cut(s)	231
PspN4I	GGNNCC	2 cut(s)	48, 804
PspPI	GGNCC	6 cut(s)	46, 62, 331, 916, 986, 1094
PsuI	RGATCY	2 cut(s)	210, 370
PvuII	CAGCTG	1 cut(s)	1100
RseI	CAYNNNNRTG	1 cut(s)	772
SaqAI	TTAA	5 cut(s)	291, 462, 636, 771, 785
SatI	GCNGC	3 cut(s)	180, 579, 675
Sau3AI	GATC	3 cut(s)	210, 297, 370
Sau96I	GGNCC	6 cut(s)	46, 62, 331, 916, 986, 1094
SchI	GAGTC	3 cut(s)	433, 869, 947
ScrFI	CCNGG	1 cut(s)	233
SduI	GDGCHC	2 cut(s)	122, 548
SfaNI	GCATC	3 cut(s)	397, 589, 620
SfcI	CTRYAG	1 cut(s)	85
SfuI	TTCGAA	1 cut(s)	659
SinI	GGWCC	3 cut(s)	46, 986, 1094
SmiMI	CAYNNNNRTG	1 cut(s)	772
Sse9I	AATT	6 cut(s)	124, 387, 523, 632, 943, 1055
SseBI	AGGCCT	1 cut(s)	111
SsiI	CCGC	5 cut(s)	69, 145, 180, 205, 688
SspI	AATATT	1 cut(s)	346
SspMI	CTAG	2 cut(s)	107, 920
StuI	AGGCCT	1 cut(s)	111
StyD4I	CCNGG	1 cut(s)	231
StyI	CCWWGG	1 cut(s)	1090
TaaI	ACNGT	1 cut(s)	553
TaiI	ACGT	4 cut(s)	231, 852, 1041, 1064
TaqI	TCGA	2 cut(s)	256, 659
TasI	AATT	6 cut(s)	124, 387, 523, 632, 943, 1055
TauI	GCSGC	1 cut(s)	182
Tru1I	TTAA	5 cut(s)	291, 462, 636, 771, 785
Tru9I	TTAA	5 cut(s)	291, 462, 636, 771, 785
TscAI	CASTG	2 cut(s)	594, 621
TseFI	GTSAC	2 cut(s)	154, 1000
TseI	GCWGC	2 cut(s)	578, 674
Tsp45I	GTSAC	2 cut(s)	154, 1000
TspDTI	ATGAA	6 cut(s)	450, 485, 588, 684, 737, 1031
TspRI	CASTG	2 cut(s)	594, 621
VpaK11BI	GGWCC	3 cut(s)	46, 986, 1094
XapI	RAATTY	3 cut(s)	387, 523, 632
XmiI	GTMKAC	2 cut(s)	873, 1064
XspI	CTAG	2 cut(s)	107, 920
ZraI	GACGTC	1 cut(s)	1062

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.