FvH4_5g18050

Germin-like protein subfamily 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000366
Physical Location & Seq
Reverse (-)
10375223 .. 10376276
1054 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18050.t1

Sequence Viewer

Length: 693 bp
ATGATGAAAGGTGCTCATTTCCCTGCTACAACTGCTGCTTTTGCCCTACTGGCATTGGCAACCTTCCACCTTGTCTCTGCCTCTGATCCTAGTTCTCTCCAAGATTTCTGTGTAGCACTTAAGAACACTGATGTGTTTGTGAATGGGAAATTCTGCAAGGACCCAAAGCTTGTAACAGAAGACGATTTCTTCTTTGACGGGCTCCGGATGCCCGGAAACACATCAAATGCGGTTGGTTCCAAGGTCACCCCGGCGAATGTGGAGCAAATAGCAGGGCTGAACACTCTTGGGATATCCCTTGCTCGCATAGACTATGCACCAAATGGCGGACTAAACCCTCCTCACACCCACCCTCGGGCCTCGGAGTTTCTTGTGGTCATGGAAGGCACTCTCTACGTTGGCTTCGTCACATCCAACTCTGACAACGGCAACAAGCTATTCACCAAAGTGTTGTACCCTGGAGATGTGTTTGTCTTCCCGGTTGGTCTTATTCACTTCCAGTTCAACATCGGAAAGGTCAATGCCGTGGCCATTGCCGGTCTTAGCAGCCAGAACCCAGGTGTCATCACCATAGCCAACGCCGTTTTCGGCTCCAAACCTCCGATCAAGCTTGATGTTCTCACCAAGGCCTTCCAAGTTGAAAGCAATGTCGTTGAGTATCTCCAGAACCAGTTCTGGTATGACAACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

231

Amino Acids

24.75

Weight (kDa)

5.91

Isoelectric Point (pI)

22.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 67 - 218 1.6e-51 Cupin
Cupin_2 PF07883 103 - 177 1.8e-07 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05950 AT5G38930 AT5G38940 AT5G38940 AT5G39130 AT5G39160 AT5G39160 AT5G39160 AT5G39190 AT5G39190
fragaria_vesca FvH4_2g10250 FvH4_3g20400 FvH4_5g18050 FvH4_5g18070 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18470
malus_domestica MD06G1238100.v1.1 MD10G1022500.v1.1 MD10G1022600.v1.1 MD10G1022900.v1.1 MD10G1023100.v1.1 MD10G1023500.v1.1 MD14G1243000.v1.1 MD14G1243100.v1.1
prunus_persica Prupe.5G240700_v2.0.a1 Prupe.8G025500_v2.0.a1 Prupe.8G025600_v2.0.a1 Prupe.8G025700_v2.0.a1 Prupe.8G025800_v2.0.a1 Prupe.8G025900_v2.0.a1 Prupe.8G035600_v2.0.a1 Prupe.8G035700_v2.0.a1 Prupe.8G035800_v2.0.a1 Prupe.8G035900_v2.0.a1 Prupe.8G048300_v2.0.a1
pyrus_communis pycom06g21150 pycom06g21360 pycom06g21370 pycom14g09160 pycom14g09170 pycom14g20410 pycom14g20430 pycom14g20440
rosa_chinensis RchiOBHm_Chr1g0318151 RchiOBHm_Chr1g0354011 RchiOBHm_Chr1g0354031 RchiOBHm_Chr5g0034291 RchiOBHm_Chr7g0177551
rosa_laevigata RLG00000017504 RLG00000028234 RLG00000028236 RLG00000033537
rosa_multiflora Rmu_sc0004015.1_g000016 Rmu_sc0011424.1_g000001 Rmu_sc0011424.1_g000017 Rmu_sc0022466.1_g000003 Rmu_sc0033434.1_g000011 Rmu_ssc0000330.1_g000005 Rmu_ssc0000330.1_g000011
rosa_roxburghii Rroxscaffold_1G00045980 Rroxscaffold_2G00137150 Rroxscaffold_3G00275760 Rroxscaffold_4G00301290 Rroxscaffold_4G00301300 Rroxscaffold_4G00301320 Rroxscaffold_4G00301340 Rroxscaffold_4G00329570 Rroxscaffold_4G00329610 Rroxscaffold_4G00329760 Rroxscaffold_4G00329840 Rroxscaffold_4G00329920
rosa_rugosa Rorug01G0240100 Rorug01G0240200 Rorug01G0240500 Rorug06G0407200 Rorug06G0407300
rosa_samantha Rh1AG031100 Rh1AG251500 Rh1BG223100 Rh1CG029900 Rh1CG235700 Rh1CG235900 Rh1DG250000 Rh2BG197800 Rh5AG236600 Rh5BG237100 Rh5CG266900 Rh5DG244600 Rh7AG006500 Rh7BG006500 Rh7CG006900 Rh7CG007000 Rh7DG006500 Rh7DG006600
rosa_wichuraiana Rw1G002460 Rw1G002500 Rw1G021930 Rw2G014580 Rw7G000540 Rw7G000560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 204
AciI CCGC 2 cut(s) 230, 327
AclWI GGATC 1 cut(s) 80
AcoI YGGCCR 1 cut(s) 528
AcsI RAATTY 1 cut(s) 149
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 3 cut(s) 326, 354, 355
AflII CTTAAG 1 cut(s) 119
AgsI TTSAA 2 cut(s) 505, 641
AjnI CCWGG 2 cut(s) 457, 556
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AleI CACNNNNGTG 2 cut(s) 131, 446
AluBI AGCT 3 cut(s) 169, 436, 610
AluI AGCT 3 cut(s) 169, 436, 610
Alw21I GWGCWC 1 cut(s) 16
Alw26I GTCTC 1 cut(s) 79
AlwI GGATC 1 cut(s) 80
Ama87I CYCGRG 1 cut(s) 354
Aor13HI TCCGGA 1 cut(s) 204
AoxI GGCC 3 cut(s) 357, 528, 627
ApeKI GCWGC 2 cut(s) 35, 546
ApoI RAATTY 1 cut(s) 149
Asp700I GAANNNNTTC 1 cut(s) 671
AspS9I GGNCC 2 cut(s) 160, 357
AsuC2I CCSGG 3 cut(s) 213, 251, 479
AsuHPI GGTGA 4 cut(s) 238, 433, 559, 613
AvaI CYCGRG 1 cut(s) 354
AvaII GGWCC 1 cut(s) 160
BalI TGGCCA 1 cut(s) 530
BanII GRGCYC 1 cut(s) 204
BbsI GAAGAC 2 cut(s) 186, 466
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 2 cut(s) 22, 558
BceAI ACGGC 3 cut(s) 442, 509, 566
BciT130I CCWGG 2 cut(s) 459, 558
BcnI CCSGG 3 cut(s) 213, 251, 479
BcoDI GTCTC 1 cut(s) 79
BfaI CTAG 2 cut(s) 90, 691
BfrI CTTAAG 1 cut(s) 119
BglI GCCNNNNNGGC 1 cut(s) 50
BisI GCNGC 2 cut(s) 36, 547
BlsI GCNGC 2 cut(s) 37, 548
Bme1390I CCNGG 5 cut(s) 213, 251, 459, 479, 558
Bme18I GGWCC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 354
BmgT120I GGNCC 2 cut(s) 160, 357
BmiI GGNNCC 4 cut(s) 162, 203, 238, 592
BmrFI CCNGG 5 cut(s) 213, 251, 459, 479, 558
BmsI GCATC 1 cut(s) 198
BpiI GAAGAC 2 cut(s) 186, 466
BpmI CTGGAG 2 cut(s) 480, 647
BpuMI CCSGG 3 cut(s) 213, 251, 479
BsaJI CCNNGG 8 cut(s) 240, 249, 353, 360, 457, 525, 556, 624
BsaWI WCCGGW 1 cut(s) 204
Bsc4I CCNNNNNNNGG 3 cut(s) 326, 354, 355
Bse118I RCCGGY 1 cut(s) 536
Bse1I ACTGG 3 cut(s) 54, 499, 670
Bse3DI GCAATG 2 cut(s) 531, 652
BseAI TCCGGA 1 cut(s) 204
BseBI CCWGG 2 cut(s) 459, 558
BseDI CCNNGG 8 cut(s) 240, 249, 353, 360, 457, 525, 556, 624
BseGI GGATG 2 cut(s) 213, 410
BseLI CCNNNNNNNGG 3 cut(s) 326, 354, 355
BseMI GCAATG 2 cut(s) 531, 652
BseNI ACTGG 3 cut(s) 54, 499, 670
BseRI GAGGAG 1 cut(s) 330
BseXI GCAGC 2 cut(s) 22, 558
BshFI GGCC 3 cut(s) 359, 530, 629
BsiHKAI GWGCWC 1 cut(s) 16
BsiHKCI CYCGRG 1 cut(s) 354
BsiSI CCGG 5 cut(s) 205, 213, 251, 479, 537
BslI CCNNNNNNNGG 3 cut(s) 326, 354, 355
BsmAI GTCTC 1 cut(s) 79
BsnI GGCC 3 cut(s) 359, 530, 629
BsoBI CYCGRG 1 cut(s) 354
Bsp1286I GDGCHC 2 cut(s) 16, 204
Bsp13I TCCGGA 1 cut(s) 204
Bsp143I GATC 2 cut(s) 85, 603
BspACI CCGC 2 cut(s) 230, 327
BspANI GGCC 3 cut(s) 359, 530, 629
BspEI TCCGGA 1 cut(s) 204
BspLI GGNNCC 4 cut(s) 162, 203, 238, 592
BspPI GGATC 1 cut(s) 80
BspTI CTTAAG 1 cut(s) 119
BsrDI GCAATG 2 cut(s) 531, 652
BsrFI RCCGGY 1 cut(s) 536
BsrI ACTGG 3 cut(s) 54, 499, 670
BssAI RCCGGY 1 cut(s) 536
BssECI CCNNGG 8 cut(s) 240, 249, 353, 360, 457, 525, 556, 624
BssMI GATC 2 cut(s) 85, 603
BssT1I CCWWGG 2 cut(s) 240, 624
Bst2UI CCWGG 2 cut(s) 459, 558
BstAFI CTTAAG 1 cut(s) 119
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 1 cut(s) 542
BstDSI CCRYGG 1 cut(s) 525
BstEII GGTNACC 1 cut(s) 244
BstF5I GGATG 2 cut(s) 213, 410
BstKTI GATC 2 cut(s) 88, 606
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 2 cut(s) 85, 603
BstMWI GCNNNNNNNGC 4 cut(s) 32, 41, 50, 208
BstNI CCWGG 2 cut(s) 459, 558
BstPI GGTNACC 1 cut(s) 244
BstSCI CCNGG 5 cut(s) 211, 249, 457, 477, 556
BstV1I GCAGC 2 cut(s) 22, 558
BstV2I GAAGAC 2 cut(s) 186, 466
BsuRI GGCC 3 cut(s) 359, 530, 629
BtgI CCRYGG 1 cut(s) 525
BtsCI GGATG 2 cut(s) 213, 410
BtsIMutI CAGTG 1 cut(s) 126
Cac8I GCNNGC 1 cut(s) 304
Cfr10I RCCGGY 1 cut(s) 536
Cfr13I GGNCC 2 cut(s) 160, 357
Csp6I GTAC 1 cut(s) 454
CviAII CATG 1 cut(s) 379
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 1 cut(s) 542
DpnI GATC 2 cut(s) 87, 605
DpnII GATC 2 cut(s) 85, 603
EaeI YGGCCR 1 cut(s) 528
EciI GGCGGA 1 cut(s) 342
Eco130I CCWWGG 2 cut(s) 240, 624
Eco147I AGGCCT 1 cut(s) 629
Eco24I GRGCYC 1 cut(s) 204
Eco32I GATATC 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 354
Eco91I GGTNACC 1 cut(s) 244
EcoO109I RGGNCCY 1 cut(s) 160
EcoO65I GGTNACC 1 cut(s) 244
EcoRII CCWGG 2 cut(s) 457, 556
EcoRV GATATC 1 cut(s) 294
EcoT14I CCWWGG 2 cut(s) 240, 624
EcoT38I GRGCYC 1 cut(s) 204
ErhI CCWWGG 2 cut(s) 240, 624
FaeI CATG 1 cut(s) 382
FaiI YATR 5 cut(s) 308, 315, 380, 572, 681
FatI CATG 1 cut(s) 378
Fnu4HI GCNGC 2 cut(s) 36, 547
FokI GGATG 2 cut(s) 220, 397
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 2 cut(s) 36, 547
FspBI CTAG 2 cut(s) 90, 691
GluI GCNGC 2 cut(s) 36, 547
GsuI CTGGAG 2 cut(s) 480, 647
HaeIII GGCC 3 cut(s) 359, 530, 629
HapII CCGG 5 cut(s) 205, 213, 251, 479, 537
Hin1II CATG 1 cut(s) 382
HindIII AAGCTT 2 cut(s) 167, 608
HpaII CCGG 5 cut(s) 205, 213, 251, 479, 537
HphI GGTGA 4 cut(s) 238, 433, 559, 613
Hpy188I TCNGA 5 cut(s) 85, 364, 421, 512, 603
Hpy188III TCNNGA 2 cut(s) 205, 664
HpyAV CCTTC 3 cut(s) 73, 377, 640
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 2 cut(s) 156, 317
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 41, 50, 208
HpyF3I CTNAG 1 cut(s) 542
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 1 cut(s) 382
Kpn2I TCCGGA 1 cut(s) 204
Kzo9I GATC 2 cut(s) 85, 603
LmnI GCTCC 3 cut(s) 207, 262, 596
Lsp1109I GCAGC 2 cut(s) 22, 558
LweI GCATC 1 cut(s) 198
MaeI CTAG 2 cut(s) 90, 691
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 3 cut(s) 172, 244, 406
MalI GATC 2 cut(s) 87, 605
MboI GATC 2 cut(s) 85, 603
MboII GAAGA 3 cut(s) 181, 191, 466
MhlI GDGCHC 2 cut(s) 16, 204
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 1 cut(s) 149
MluNI TGGCCA 1 cut(s) 530
MmeI TCCRAC 1 cut(s) 438
MnlI CCTC 6 cut(s) 91, 348, 351, 363, 370, 609
Mox20I TGGCCA 1 cut(s) 530
MroI TCCGGA 1 cut(s) 204
MroXI GAANNNNTTC 1 cut(s) 671
MscI TGGCCA 1 cut(s) 530
MseI TTAA 1 cut(s) 120
MslI CAYNNNNRTG 2 cut(s) 131, 446
Msp20I TGGCCA 1 cut(s) 530
MspCI CTTAAG 1 cut(s) 119
MspI CCGG 5 cut(s) 205, 213, 251, 479, 537
MspR9I CCNGG 5 cut(s) 213, 251, 459, 479, 558
MvaI CCWGG 2 cut(s) 459, 558
MwoI GCNNNNNNNGC 4 cut(s) 32, 41, 50, 208
NciI CCSGG 3 cut(s) 213, 251, 479
NdeII GATC 2 cut(s) 85, 603
NlaIII CATG 1 cut(s) 382
NlaIV GGNNCC 4 cut(s) 162, 203, 238, 592
NmuCI GTSAC 2 cut(s) 244, 406
OliI CACNNNNGTG 2 cut(s) 131, 446
PceI AGGCCT 1 cut(s) 629
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PdmI GAANNNNTTC 1 cut(s) 671
PkrI GCNGC 2 cut(s) 37, 548
PpuMI RGGWCCY 1 cut(s) 160
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 2 cut(s) 457, 556
PspEI GGTNACC 1 cut(s) 244
PspGI CCWGG 2 cut(s) 457, 556
PspN4I GGNNCC 4 cut(s) 162, 203, 238, 592
PspPI GGNCC 2 cut(s) 160, 357
PspPPI RGGWCCY 1 cut(s) 160
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
RseI CAYNNNNRTG 2 cut(s) 131, 446
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 2 cut(s) 36, 547
Sau3AI GATC 2 cut(s) 85, 603
Sau96I GGNCC 2 cut(s) 160, 357
ScrFI CCNGG 5 cut(s) 213, 251, 459, 479, 558
SduI GDGCHC 2 cut(s) 16, 204
SfaNI GCATC 1 cut(s) 198
SinI GGWCC 1 cut(s) 160
SmiMI CAYNNNNRTG 2 cut(s) 131, 446
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 1 cut(s) 149
SseBI AGGCCT 1 cut(s) 629
SsiI CCGC 2 cut(s) 230, 327
SspMI CTAG 2 cut(s) 90, 691
StuI AGGCCT 1 cut(s) 629
StyD4I CCNGG 5 cut(s) 211, 249, 457, 477, 556
StyI CCWWGG 2 cut(s) 240, 624
TaiI ACGT 1 cut(s) 399
TasI AATT 1 cut(s) 149
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 2 cut(s) 244, 406
TseI GCWGC 2 cut(s) 35, 546
Tsp45I GTSAC 2 cut(s) 244, 406
TspDTI ATGAA 1 cut(s) 20
TspRI CASTG 1 cut(s) 133
Vha464I CTTAAG 1 cut(s) 119
VpaK11BI GGWCC 1 cut(s) 160
XapI RAATTY 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 671
XspI CTAG 2 cut(s) 90, 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.