MD10G1023500.v1.1

Germin-like protein subfamily 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000366
Physical Location & Seq
Reverse (-)
2949863 .. 2951029
1167 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1023500.v1.1.491

Sequence Viewer

Length: 678 bp
ATGAAAGGTGCTCAGTTCCTCGTATGCATTGTTGCCATCTTGGCATTCGCAACCTCCCTTGTCTCTGCCTCTGACCCCAGTCCCCTCCAGGACTTCTGCGTTGCACTTAATGACACCAAATTAGGGGTGTTTGTGAACGGGAAATTCTGCAAGGACTCAAGGCTTGCATCAGCAAATGATTTCTTCTTTTCTGGGCTTCAAATTCCAAGAAGCACACAAAATCCGTTGGGTTCAACGGTAACACCTGCTAATGTGGATCAAATAGCAGGACTAAACACTCTCGGCATATCCCTAGCTCGCATAGACTTTGCACCAAATGGCCTAAACCCTCCCCACACTCACCCTCGCGGCACGGAAATCCTTTTTCTCCAGGAAGGTACACTCTACGTTGGGTTCGTAACGTCCAACCCAAATAATCGTCTATTCACCAAGGTGTTGAACAAGGGAGATGTGTTTGTGTTCCCAGTTGGTCTTATTCACTTCCAACTGAATGTGGGACATACCAGTGCTGTCGCCATTGCTGGTCTTAGCAGCCAGAATCCAGGAGTCATCACCATAGCAAATGCAGTGTTCGGCGCCAACCCTCCCATCAATCCTGATGTTCTAGCCAAGGCCTTCCAAGTCGATGACAAACTGGTTTCATATCTTCAAAAACAGTTCTGGTACGACAACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

226

Amino Acids

24.11

Weight (kDa)

6.89

Isoelectric Point (pI)

21.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 65 - 212 1.2e-52 Cupin
Cupin_2 PF07883 101 - 172 6.9e-07 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05950 AT5G38930 AT5G38940 AT5G38940 AT5G39130 AT5G39160 AT5G39160 AT5G39160 AT5G39190 AT5G39190
fragaria_vesca FvH4_2g10250 FvH4_3g20400 FvH4_5g18050 FvH4_5g18070 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18470
malus_domestica MD06G1238100.v1.1 MD10G1022500.v1.1 MD10G1022600.v1.1 MD10G1022900.v1.1 MD10G1023100.v1.1 MD10G1023500.v1.1 MD14G1243000.v1.1 MD14G1243100.v1.1
prunus_persica Prupe.5G240700_v2.0.a1 Prupe.8G025500_v2.0.a1 Prupe.8G025600_v2.0.a1 Prupe.8G025700_v2.0.a1 Prupe.8G025800_v2.0.a1 Prupe.8G025900_v2.0.a1 Prupe.8G035600_v2.0.a1 Prupe.8G035700_v2.0.a1 Prupe.8G035800_v2.0.a1 Prupe.8G035900_v2.0.a1 Prupe.8G048300_v2.0.a1
pyrus_communis pycom06g21150 pycom06g21360 pycom06g21370 pycom14g09160 pycom14g09170 pycom14g20410 pycom14g20430 pycom14g20440
rosa_chinensis RchiOBHm_Chr1g0318151 RchiOBHm_Chr1g0354011 RchiOBHm_Chr1g0354031 RchiOBHm_Chr5g0034291 RchiOBHm_Chr7g0177551
rosa_laevigata RLG00000017504 RLG00000028234 RLG00000028236 RLG00000033537
rosa_multiflora Rmu_sc0004015.1_g000016 Rmu_sc0011424.1_g000001 Rmu_sc0011424.1_g000017 Rmu_sc0022466.1_g000003 Rmu_sc0033434.1_g000011 Rmu_ssc0000330.1_g000005 Rmu_ssc0000330.1_g000011
rosa_roxburghii Rroxscaffold_1G00045980 Rroxscaffold_2G00137150 Rroxscaffold_3G00275760 Rroxscaffold_4G00301290 Rroxscaffold_4G00301300 Rroxscaffold_4G00301320 Rroxscaffold_4G00301340 Rroxscaffold_4G00329570 Rroxscaffold_4G00329610 Rroxscaffold_4G00329760 Rroxscaffold_4G00329840 Rroxscaffold_4G00329920
rosa_rugosa Rorug01G0240100 Rorug01G0240200 Rorug01G0240500 Rorug06G0407200 Rorug06G0407300
rosa_samantha Rh1AG031100 Rh1AG251500 Rh1BG223100 Rh1CG029900 Rh1CG235700 Rh1CG235900 Rh1DG250000 Rh2BG197800 Rh5AG236600 Rh5BG237100 Rh5CG266900 Rh5DG244600 Rh7AG006500 Rh7BG006500 Rh7CG006900 Rh7CG007000 Rh7DG006500 Rh7DG006600
rosa_wichuraiana Rw1G002460 Rw1G002500 Rw1G021930 Rw2G014580 Rw7G000540 Rw7G000560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 253
Acc36I ACCTGC 1 cut(s) 253
AccB1I GGYRCC 1 cut(s) 575
AccII CGCG 1 cut(s) 348
AciI CCGC 1 cut(s) 348
AclWI GGATC 1 cut(s) 264
AcsI RAATTY 2 cut(s) 143, 201
AcyI GRCGYC 1 cut(s) 576
AfaI GTAC 2 cut(s) 379, 665
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 4 cut(s) 200, 234, 439, 650
AjnI CCWGG 3 cut(s) 87, 369, 541
AleI CACNNNNGTG 1 cut(s) 431
AluBI AGCT 1 cut(s) 296
AluI AGCT 1 cut(s) 296
Alw21I GWGCWC 1 cut(s) 13
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 1 cut(s) 264
AoxI GGCC 2 cut(s) 319, 612
ApeKI GCWGC 1 cut(s) 531
ApoI RAATTY 2 cut(s) 143, 201
AspLEI GCGC 1 cut(s) 578
AsuHPI GGTGA 3 cut(s) 332, 418, 544
BanI GGYRCC 1 cut(s) 575
Bbv12I GWGCWC 1 cut(s) 13
BbvI GCAGC 1 cut(s) 543
BccI CCATC 2 cut(s) 44, 596
BciT130I CCWGG 3 cut(s) 89, 371, 543
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 2 cut(s) 293, 605
BfoI RGCGCY 1 cut(s) 579
BfuAI ACCTGC 1 cut(s) 253
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 2 cut(s) 349, 532
BlsI GCNGC 2 cut(s) 350, 533
Bme1390I CCNGG 3 cut(s) 89, 371, 543
BmiI GGNNCC 1 cut(s) 577
BmrFI CCNGG 3 cut(s) 89, 371, 543
BmrI ACTGGG 2 cut(s) 72, 458
BmsI GCATC 1 cut(s) 176
BmuI ACTGGG 2 cut(s) 72, 458
BoxI GACNNNNGTC 1 cut(s) 78
BpmI CTGGAG 2 cut(s) 71, 353
BpuEI CTTGAG 1 cut(s) 142
BsaHI GRCGYC 1 cut(s) 576
BsaJI CCNNGG 2 cut(s) 429, 609
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse1I ACTGG 4 cut(s) 78, 464, 504, 639
Bse3DI GCAATG 1 cut(s) 516
BseBI CCWGG 3 cut(s) 89, 371, 543
BseDI CCNNGG 2 cut(s) 429, 609
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMI GCAATG 1 cut(s) 516
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 4 cut(s) 78, 464, 504, 639
BseXI GCAGC 1 cut(s) 543
Bsh1236I CGCG 1 cut(s) 348
BshFI GGCC 2 cut(s) 321, 614
BshNI GGYRCC 1 cut(s) 575
BsiHKAI GWGCWC 1 cut(s) 13
BslFI GGGAC 2 cut(s) 66, 510
BslI CCNNNNNNNGG 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 67
BsmFI GGGAC 2 cut(s) 66, 510
BsmI GAATGC 1 cut(s) 44
BsnI GGCC 2 cut(s) 321, 614
Bsp1286I GDGCHC 1 cut(s) 13
Bsp143I GATC 1 cut(s) 256
BspACI CCGC 1 cut(s) 348
BspANI GGCC 2 cut(s) 321, 614
BspCNI CTCAG 1 cut(s) 25
BspFNI CGCG 1 cut(s) 348
BspLI GGNNCC 1 cut(s) 577
BspMI ACCTGC 1 cut(s) 253
BspPI GGATC 1 cut(s) 264
BspT107I GGYRCC 1 cut(s) 575
BsrDI GCAATG 1 cut(s) 516
BsrI ACTGG 4 cut(s) 78, 464, 504, 639
BssECI CCNNGG 2 cut(s) 429, 609
BssMI GATC 1 cut(s) 256
BssNI GRCGYC 1 cut(s) 576
BssT1I CCWWGG 2 cut(s) 429, 609
Bst2UI CCWGG 3 cut(s) 89, 371, 543
Bst4CI ACNGT 2 cut(s) 238, 657
BstACI GRCGYC 1 cut(s) 576
BstC8I GCNNGC 2 cut(s) 165, 298
BstDEI CTNAG 2 cut(s) 12, 527
BstFNI CGCG 1 cut(s) 348
BstH2I RGCGCY 1 cut(s) 579
BstHHI GCGC 1 cut(s) 578
BstKTI GATC 1 cut(s) 259
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 1 cut(s) 256
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstNI CCWGG 3 cut(s) 89, 371, 543
BstPAI GACNNNNGTC 1 cut(s) 78
BstSCI CCNGG 3 cut(s) 87, 369, 541
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 1 cut(s) 543
BsuRI GGCC 2 cut(s) 321, 614
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 2 cut(s) 511, 573
BveI ACCTGC 1 cut(s) 253
Cac8I GCNNGC 2 cut(s) 165, 298
CfoI GCGC 1 cut(s) 578
Csp6I GTAC 2 cut(s) 378, 664
CviJI RGCY 7 cut(s) 163, 196, 296, 321, 534, 608, 614
CviKI_1 RGCY 7 cut(s) 163, 196, 296, 321, 534, 608, 614
CviQI GTAC 2 cut(s) 378, 664
DdeI CTNAG 2 cut(s) 12, 527
DinI GGCGCC 1 cut(s) 577
DpnI GATC 1 cut(s) 258
DpnII GATC 1 cut(s) 256
Eco130I CCWWGG 2 cut(s) 429, 609
Eco147I AGGCCT 1 cut(s) 614
EcoRII CCWGG 3 cut(s) 87, 369, 541
EcoT14I CCWWGG 2 cut(s) 429, 609
EcoT22I ATGCAT 1 cut(s) 29
EgeI GGCGCC 1 cut(s) 577
EheI GGCGCC 1 cut(s) 577
ErhI CCWWGG 2 cut(s) 429, 609
FaiI YATR 6 cut(s) 25, 287, 302, 501, 557, 643
FaqI GGGAC 2 cut(s) 66, 510
Fnu4HI GCNGC 2 cut(s) 349, 532
Fsp4HI GCNGC 2 cut(s) 349, 532
FspBI CTAG 2 cut(s) 293, 605
GlaI GCGC 1 cut(s) 577
GluI GCNGC 2 cut(s) 349, 532
GsuI CTGGAG 2 cut(s) 71, 353
HaeII RGCGCY 1 cut(s) 579
HaeIII GGCC 2 cut(s) 321, 614
HhaI GCGC 1 cut(s) 578
Hin1I GRCGYC 1 cut(s) 576
Hin6I GCGC 1 cut(s) 576
HinP1I GCGC 1 cut(s) 576
HinfI GANTC 3 cut(s) 155, 538, 546
HphI GGTGA 3 cut(s) 332, 418, 544
Hpy166II GTNNAC 2 cut(s) 136, 380
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 1 cut(s) 596
Hpy8I GTNNAC 2 cut(s) 136, 380
HpyAV CCTTC 2 cut(s) 368, 625
HpyCH4III ACNGT 2 cut(s) 238, 657
HpyCH4IV ACGT 2 cut(s) 387, 401
HpyCH4V TGCA 6 cut(s) 27, 104, 150, 167, 311, 566
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 2 cut(s) 12, 527
HpySE526I ACGT 2 cut(s) 387, 401
Hsp92I GRCGYC 1 cut(s) 576
HspAI GCGC 1 cut(s) 576
KasI GGCGCC 1 cut(s) 575
Kzo9I GATC 1 cut(s) 256
Lsp1109I GCAGC 1 cut(s) 543
LweI GCATC 1 cut(s) 176
MaeI CTAG 2 cut(s) 293, 605
MaeII ACGT 2 cut(s) 387, 401
MaeIII GTNAC 2 cut(s) 238, 397
MalI GATC 1 cut(s) 258
MboI GATC 1 cut(s) 256
MboII GAAGA 2 cut(s) 175, 638
MhlI GDGCHC 1 cut(s) 13
MluCI AATT 3 cut(s) 119, 143, 201
Mly113I GGCGCC 1 cut(s) 576
MlyI GAGTC 2 cut(s) 149, 555
MmeI TCCRAC 2 cut(s) 429, 508
MnlI CCTC 7 cut(s) 29, 64, 79, 95, 339, 354, 594
Mph1103I ATGCAT 1 cut(s) 29
MseI TTAA 1 cut(s) 108
MslI CAYNNNNRTG 2 cut(s) 431, 504
MspR9I CCNGG 3 cut(s) 89, 371, 543
Mva1269I GAATGC 1 cut(s) 44
MvaI CCWGG 3 cut(s) 89, 371, 543
MvnI CGCG 1 cut(s) 348
MwoI GCNNNNNNNGC 1 cut(s) 41
NarI GGCGCC 1 cut(s) 576
NdeII GATC 1 cut(s) 256
NlaIV GGNNCC 1 cut(s) 577
NmeAIII GCCGAG 1 cut(s) 261
NsiI ATGCAT 1 cut(s) 29
OliI CACNNNNGTG 1 cut(s) 431
PaqCI CACCTGC 1 cut(s) 253
PceI AGGCCT 1 cut(s) 614
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PctI GAATGC 1 cut(s) 44
PfeI GAWTC 1 cut(s) 538
PfoI TCCNGGA 3 cut(s) 87, 369, 541
PkrI GCNGC 2 cut(s) 350, 533
PleI GAGTC 2 cut(s) 149, 554
PluTI GGCGCC 1 cut(s) 579
PpsI GAGTC 2 cut(s) 149, 554
PshAI GACNNNNGTC 1 cut(s) 78
Psp6I CCWGG 3 cut(s) 87, 369, 541
PspGI CCWGG 3 cut(s) 87, 369, 541
PspN4I GGNNCC 1 cut(s) 577
RsaI GTAC 2 cut(s) 379, 665
RsaNI GTAC 2 cut(s) 378, 664
RseI CAYNNNNRTG 2 cut(s) 431, 504
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 2 cut(s) 349, 532
Sau3AI GATC 1 cut(s) 256
SchI GAGTC 2 cut(s) 149, 555
ScrFI CCNGG 3 cut(s) 89, 371, 543
SduI GDGCHC 1 cut(s) 13
SetI ASST 8 cut(s) 10, 56, 247, 298, 379, 390, 404, 435
SfaNI GCATC 1 cut(s) 176
SfoI GGCGCC 1 cut(s) 577
SmiMI CAYNNNNRTG 2 cut(s) 431, 504
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
Sse9I AATT 3 cut(s) 119, 143, 201
SseBI AGGCCT 1 cut(s) 614
SsiI CCGC 1 cut(s) 348
SspDI GGCGCC 1 cut(s) 575
SspMI CTAG 2 cut(s) 293, 605
StuI AGGCCT 1 cut(s) 614
StyD4I CCNGG 3 cut(s) 87, 369, 541
StyI CCWWGG 2 cut(s) 429, 609
TaaI ACNGT 2 cut(s) 238, 657
TaiI ACGT 2 cut(s) 390, 404
TaqI TCGA 1 cut(s) 624
TasI AATT 3 cut(s) 119, 143, 201
TauI GCSGC 1 cut(s) 351
TfiI GAWTC 1 cut(s) 538
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TscAI CASTG 2 cut(s) 511, 573
TseI GCWGC 1 cut(s) 531
TspDTI ATGAA 2 cut(s) 17, 630
TspGWI ACGGA 2 cut(s) 213, 368
TspRI CASTG 2 cut(s) 511, 573
XapI RAATTY 2 cut(s) 143, 201
XspI CTAG 2 cut(s) 293, 605
Zsp2I ATGCAT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.