Rw7G000540

Germin-like protein subfamily 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000366
Physical Location & Seq
Forward (+)
458720 .. 459535
816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G000540.1

Sequence Viewer

Length: 696 bp
ATGAAAGGTGCTTATTTCCCTACTGCTGCTGCTTTTGCCCTGTTGGCATTGGCAACCTTCCTCCTTGTTTCTGCTTCTGATCCTAGTCCTCTCCAAGATTTCTGTGTAGCACTTAACACCACCCAAACTGCTATTTCTGCTGTGTTTGTGAATGGGAAATTTTGCAAGGACCCAAAGATGGCAACAGCGGATGATTTCTTTTTTTCGGGGCTCCAGATTCCTCGAAACACATCAAATCCGGTTGGTTCCAACGTCACCGCAGTAAACGTGGAGCAAATAGCAGGACTCAACACTCTTGGGATATCCCTGGCTCGCATAGACTATGCACCAAATGGCGGACTAAACCCTCCTCACACCCACCCTCGGGCCACGGAAATCCTTGTCGTCTTGGAAGGCACTCTCTACGTTGGCTTCGTCACATCCAACCCGGACAACCGCCTCATCACCAAAGTGTTGTACAAGGGAGATGTGTTTGTCTTCCCAGTTGGTCTCATTCACTTCCAGTTCAACGTCGGAAAGGTCAATGCCGTAGCCATTGCCGGTCTTAGCAGCCAGAATCCAGGTGTCATCACCATAGCCAATGCAGTGTTCGGCTCCAACCCTCCAATCAACCCTGATGTGCTCACCAAGGCCTTCCAAGTTGACAACAATATCGTTCAGTATCTCCAGAAACAGTTCTGGTATGACAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

231

Amino Acids

24.74

Weight (kDa)

6.25

Isoelectric Point (pI)

22.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 70 - 219 3.4e-54 Cupin
Cupin_2 PF07883 106 - 178 8e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05950 AT5G38930 AT5G38940 AT5G38940 AT5G39130 AT5G39160 AT5G39160 AT5G39160 AT5G39190 AT5G39190
fragaria_vesca FvH4_2g10250 FvH4_3g20400 FvH4_5g18050 FvH4_5g18070 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18470
malus_domestica MD06G1238100.v1.1 MD10G1022500.v1.1 MD10G1022600.v1.1 MD10G1022900.v1.1 MD10G1023100.v1.1 MD10G1023500.v1.1 MD14G1243000.v1.1 MD14G1243100.v1.1
prunus_persica Prupe.5G240700_v2.0.a1 Prupe.8G025500_v2.0.a1 Prupe.8G025600_v2.0.a1 Prupe.8G025700_v2.0.a1 Prupe.8G025800_v2.0.a1 Prupe.8G025900_v2.0.a1 Prupe.8G035600_v2.0.a1 Prupe.8G035700_v2.0.a1 Prupe.8G035800_v2.0.a1 Prupe.8G035900_v2.0.a1 Prupe.8G048300_v2.0.a1
pyrus_communis pycom06g21150 pycom06g21360 pycom06g21370 pycom14g09160 pycom14g09170 pycom14g20410 pycom14g20430 pycom14g20440
rosa_chinensis RchiOBHm_Chr1g0318151 RchiOBHm_Chr1g0354011 RchiOBHm_Chr1g0354031 RchiOBHm_Chr5g0034291 RchiOBHm_Chr7g0177551
rosa_laevigata RLG00000017504 RLG00000028234 RLG00000028236 RLG00000033537
rosa_multiflora Rmu_sc0004015.1_g000016 Rmu_sc0011424.1_g000001 Rmu_sc0011424.1_g000017 Rmu_sc0022466.1_g000003 Rmu_sc0033434.1_g000011 Rmu_ssc0000330.1_g000005 Rmu_ssc0000330.1_g000011
rosa_roxburghii Rroxscaffold_1G00045980 Rroxscaffold_2G00137150 Rroxscaffold_3G00275760 Rroxscaffold_4G00301290 Rroxscaffold_4G00301300 Rroxscaffold_4G00301320 Rroxscaffold_4G00301340 Rroxscaffold_4G00329570 Rroxscaffold_4G00329610 Rroxscaffold_4G00329760 Rroxscaffold_4G00329840 Rroxscaffold_4G00329920
rosa_rugosa Rorug01G0240100 Rorug01G0240200 Rorug01G0240500 Rorug06G0407200 Rorug06G0407300
rosa_samantha Rh1AG031100 Rh1AG251500 Rh1BG223100 Rh1CG029900 Rh1CG235700 Rh1CG235900 Rh1DG250000 Rh2BG197800 Rh5AG236600 Rh5BG237100 Rh5CG266900 Rh5DG244600 Rh7AG006500 Rh7BG006500 Rh7CG006900 Rh7CG007000 Rh7DG006500 Rh7DG006600
rosa_wichuraiana Rw1G002460 Rw1G002500 Rw1G021930 Rw2G014580 Rw7G000540 Rw7G000560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 188, 258, 336, 436
AclWI GGATC 1 cut(s) 74
AcsI RAATTY 1 cut(s) 158
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 4 cut(s) 178, 335, 363, 364
AgsI TTSAA 1 cut(s) 508
AjnI CCWGG 2 cut(s) 306, 559
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
AleI CACNNNNGTG 1 cut(s) 449
Alw21I GWGCWC 1 cut(s) 624
Alw26I GTCTC 1 cut(s) 494
AlwI GGATC 1 cut(s) 74
Ama87I CYCGRG 1 cut(s) 363
AoxI GGCC 2 cut(s) 366, 630
ApeKI GCWGC 3 cut(s) 26, 29, 549
ApoI RAATTY 1 cut(s) 158
Asp700I GAANNNNTTC 1 cut(s) 674
AspS9I GGNCC 2 cut(s) 169, 366
AsuC2I CCSGG 1 cut(s) 428
AsuHPI GGTGA 4 cut(s) 247, 436, 562, 616
AvaI CYCGRG 1 cut(s) 363
AvaII GGWCC 1 cut(s) 169
BanII GRGCYC 1 cut(s) 213
BbsI GAAGAC 1 cut(s) 469
Bbv12I GWGCWC 1 cut(s) 624
BbvI GCAGC 3 cut(s) 13, 16, 561
BccI CCATC 1 cut(s) 172
BceAI ACGGC 1 cut(s) 512
BciT130I CCWGG 2 cut(s) 308, 561
BcnI CCSGG 1 cut(s) 428
BcoDI GTCTC 1 cut(s) 494
BfaI CTAG 1 cut(s) 84
BglI GCCNNNNNGGC 1 cut(s) 44
BisI GCNGC 3 cut(s) 27, 30, 550
BlsI GCNGC 3 cut(s) 28, 31, 551
Bme1390I CCNGG 3 cut(s) 308, 428, 561
Bme18I GGWCC 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 363
BmgT120I GGNCC 2 cut(s) 169, 366
BmiI GGNNCC 4 cut(s) 171, 212, 247, 595
BmrFI CCNGG 3 cut(s) 308, 428, 561
BmrI ACTGGG 1 cut(s) 476
BmuI ACTGGG 1 cut(s) 476
BpiI GAAGAC 1 cut(s) 469
BpmI CTGGAG 2 cut(s) 197, 650
BpuMI CCSGG 1 cut(s) 428
BsaI GGTCTC 1 cut(s) 494
BsaJI CCNNGG 4 cut(s) 306, 362, 369, 627
BsaWI WCCGGW 1 cut(s) 238
Bsc4I CCNNNNNNNGG 4 cut(s) 178, 335, 363, 364
Bse118I RCCGGY 1 cut(s) 539
Bse1I ACTGG 2 cut(s) 482, 502
Bse3DI GCAATG 1 cut(s) 534
BseBI CCWGG 2 cut(s) 308, 561
BseDI CCNNGG 4 cut(s) 306, 362, 369, 627
BseGI GGATG 2 cut(s) 196, 419
BseLI CCNNNNNNNGG 4 cut(s) 178, 335, 363, 364
BseMI GCAATG 1 cut(s) 534
BseNI ACTGG 2 cut(s) 482, 502
BseRI GAGGAG 1 cut(s) 339
BseXI GCAGC 3 cut(s) 13, 16, 561
BshFI GGCC 2 cut(s) 368, 632
BsiHKAI GWGCWC 1 cut(s) 624
BsiHKCI CYCGRG 1 cut(s) 363
BsiSI CCGG 3 cut(s) 239, 428, 540
BslI CCNNNNNNNGG 4 cut(s) 178, 335, 363, 364
BsmAI GTCTC 1 cut(s) 494
BsnI GGCC 2 cut(s) 368, 632
Bso31I GGTCTC 1 cut(s) 494
BsoBI CYCGRG 1 cut(s) 363
Bsp1286I GDGCHC 2 cut(s) 213, 624
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 1 cut(s) 79
BspACI CCGC 4 cut(s) 188, 258, 336, 436
BspANI GGCC 2 cut(s) 368, 632
BspLI GGNNCC 4 cut(s) 171, 212, 247, 595
BspPI GGATC 1 cut(s) 74
BspTNI GGTCTC 1 cut(s) 494
BsrDI GCAATG 1 cut(s) 534
BsrFI RCCGGY 1 cut(s) 539
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 2 cut(s) 482, 502
BssAI RCCGGY 1 cut(s) 539
BssECI CCNNGG 4 cut(s) 306, 362, 369, 627
BssMI GATC 1 cut(s) 79
BssT1I CCWWGG 1 cut(s) 627
Bst2UI CCWGG 2 cut(s) 308, 561
Bst4CI ACNGT 1 cut(s) 675
BstAUI TGTACA 1 cut(s) 456
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 1 cut(s) 545
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 2 cut(s) 196, 419
BstKTI GATC 1 cut(s) 82
BstMAI GTCTC 1 cut(s) 494
BstMBI GATC 1 cut(s) 79
BstMWI GCNNNNNNNGC 3 cut(s) 35, 44, 137
BstNI CCWGG 2 cut(s) 308, 561
BstSCI CCNGG 3 cut(s) 306, 426, 559
BstV1I GCAGC 3 cut(s) 13, 16, 561
BstV2I GAAGAC 1 cut(s) 469
BsuRI GGCC 2 cut(s) 368, 632
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 2 cut(s) 196, 419
BtsI GCAGTG 1 cut(s) 591
BtsIMutI CAGTG 1 cut(s) 591
Cac8I GCNNGC 1 cut(s) 313
Cfr10I RCCGGY 1 cut(s) 539
Cfr13I GGNCC 2 cut(s) 169, 366
Csp6I GTAC 1 cut(s) 457
CviJI RGCY 9 cut(s) 211, 311, 368, 411, 533, 552, 578, 594, 632
CviKI_1 RGCY 9 cut(s) 211, 311, 368, 411, 533, 552, 578, 594, 632
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 1 cut(s) 545
DpnI GATC 1 cut(s) 81
DpnII GATC 1 cut(s) 79
EciI GGCGGA 1 cut(s) 351
Eco130I CCWWGG 1 cut(s) 627
Eco147I AGGCCT 1 cut(s) 632
Eco24I GRGCYC 1 cut(s) 213
Eco31I GGTCTC 1 cut(s) 494
Eco32I GATATC 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 169
Eco88I CYCGRG 1 cut(s) 363
EcoO109I RGGNCCY 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 306, 559
EcoRV GATATC 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 627
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 627
FaiI YATR 4 cut(s) 317, 324, 575, 684
Fnu4HI GCNGC 3 cut(s) 27, 30, 550
FokI GGATG 2 cut(s) 203, 406
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 3 cut(s) 27, 30, 550
FspBI CTAG 1 cut(s) 84
GluI GCNGC 3 cut(s) 27, 30, 550
GsuI CTGGAG 2 cut(s) 197, 650
HaeIII GGCC 2 cut(s) 368, 632
HapII CCGG 3 cut(s) 239, 428, 540
HincII GTYRAC 1 cut(s) 643
HindII GTYRAC 1 cut(s) 643
HinfI GANTC 3 cut(s) 217, 285, 556
HpaII CCGG 3 cut(s) 239, 428, 540
HphI GGTGA 4 cut(s) 247, 436, 562, 616
Hpy166II GTNNAC 2 cut(s) 265, 643
Hpy188I TCNGA 2 cut(s) 79, 515
Hpy188III TCNNGA 2 cut(s) 214, 667
Hpy8I GTNNAC 2 cut(s) 265, 643
Hpy99I CGWCG 1 cut(s) 515
HpyAV CCTTC 3 cut(s) 67, 386, 643
HpyCH4III ACNGT 1 cut(s) 675
HpyCH4IV ACGT 4 cut(s) 252, 267, 405, 510
HpyCH4V TGCA 3 cut(s) 165, 326, 584
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 44, 137
HpyF3I CTNAG 1 cut(s) 545
HpySE526I ACGT 4 cut(s) 252, 267, 405, 510
Kzo9I GATC 1 cut(s) 79
LmnI GCTCC 3 cut(s) 216, 271, 599
Lsp1109I GCAGC 3 cut(s) 13, 16, 561
MaeI CTAG 1 cut(s) 84
MaeII ACGT 4 cut(s) 252, 267, 405, 510
MaeIII GTNAC 2 cut(s) 253, 415
MalI GATC 1 cut(s) 81
MboI GATC 1 cut(s) 79
MboII GAAGA 1 cut(s) 469
MhlI GDGCHC 2 cut(s) 213, 624
MluCI AATT 2 cut(s) 158, 691
MlyI GAGTC 1 cut(s) 279
MmeI TCCRAC 4 cut(s) 273, 447, 493, 621
MnlI CCTC 8 cut(s) 71, 99, 231, 357, 360, 372, 449, 612
MroXI GAANNNNTTC 1 cut(s) 674
MseI TTAA 2 cut(s) 114, 694
MslI CAYNNNNRTG 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 3 cut(s) 239, 428, 540
MspR9I CCNGG 3 cut(s) 308, 428, 561
MvaI CCWGG 2 cut(s) 308, 561
MwoI GCNNNNNNNGC 3 cut(s) 35, 44, 137
NciI CCSGG 1 cut(s) 428
NdeII GATC 1 cut(s) 79
NlaIV GGNNCC 4 cut(s) 171, 212, 247, 595
NmuCI GTSAC 2 cut(s) 253, 415
OliI CACNNNNGTG 1 cut(s) 449
PceI AGGCCT 1 cut(s) 632
PcsI WCGNNNNNNNCGW 1 cut(s) 411
PdmI GAANNNNTTC 1 cut(s) 674
PfeI GAWTC 2 cut(s) 217, 556
PkrI GCNGC 3 cut(s) 28, 31, 551
PleI GAGTC 1 cut(s) 279
PpsI GAGTC 1 cut(s) 279
PpuMI RGGWCCY 1 cut(s) 169
Psp5II RGGWCCY 1 cut(s) 169
Psp6I CCWGG 2 cut(s) 306, 559
PspGI CCWGG 2 cut(s) 306, 559
PspN4I GGNNCC 4 cut(s) 171, 212, 247, 595
PspPI GGNCC 2 cut(s) 169, 366
PspPPI RGGWCCY 1 cut(s) 169
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 2 cut(s) 114, 694
SatI GCNGC 3 cut(s) 27, 30, 550
Sau3AI GATC 1 cut(s) 79
Sau96I GGNCC 2 cut(s) 169, 366
SchI GAGTC 1 cut(s) 279
ScrFI CCNGG 3 cut(s) 308, 428, 561
SduI GDGCHC 2 cut(s) 213, 624
SetI ASST 8 cut(s) 10, 59, 255, 270, 408, 513, 522, 565
SinI GGWCC 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 449
Sse9I AATT 2 cut(s) 158, 691
SseBI AGGCCT 1 cut(s) 632
SsiI CCGC 4 cut(s) 188, 258, 336, 436
SspMI CTAG 1 cut(s) 84
StuI AGGCCT 1 cut(s) 632
StyD4I CCNGG 3 cut(s) 306, 426, 559
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 1 cut(s) 675
TaiI ACGT 4 cut(s) 255, 270, 408, 513
TaqI TCGA 1 cut(s) 223
TasI AATT 2 cut(s) 158, 691
TatI WGTACW 1 cut(s) 456
TfiI GAWTC 2 cut(s) 217, 556
Tru1I TTAA 2 cut(s) 114, 694
Tru9I TTAA 2 cut(s) 114, 694
TscAI CASTG 1 cut(s) 591
TseFI GTSAC 2 cut(s) 253, 415
TseI GCWGC 3 cut(s) 26, 29, 549
Tsp45I GTSAC 2 cut(s) 253, 415
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 386
TspRI CASTG 1 cut(s) 591
VpaK11BI GGWCC 1 cut(s) 169
XapI RAATTY 1 cut(s) 158
XmnI GAANNNNTTC 1 cut(s) 674
XspI CTAG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.