pycom14g20410

Germin-like protein subfamily 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000366
Physical Location & Seq
Reverse (-)
21459699 .. 21460281
583 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g20410.1

Sequence Viewer

Length: 375 bp
ATGAAAGGAGTTCATTTCCCTGTAACTGCTGCTGCCCTGTCAGTATTAGCATTCACTACCCTCCTTGCCTCTGCCTCTGATCCCGATCCTCTTCAGGACTTTTGCGTAGCAATTAATAACACCGATTTTGCTGTGTTTGTGAACGGGAAGTTCTGCAAGGACCCAAATCTTGCATCAGCAAACGATTTCTTCTCTGACAAGCTCCGGTACTCCGGAAACACATCGAATCCGGTCGGTTCAATTGTTACGGCGGCGAACGTGGATGTTATACCCGGACTCAACACTCTCGGCATATCCTTTGCTCGCGTAGACTTCGCACCAAATGGCCTCAATCCGCCTCACACTCACCCTCGTGCCACGGATCGATCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

125

Amino Acids

13.05

Weight (kDa)

5.77

Isoelectric Point (pI)

6.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 71 - 120 9.1e-15 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05950 AT5G38930 AT5G38940 AT5G38940 AT5G39130 AT5G39160 AT5G39160 AT5G39160 AT5G39190 AT5G39190
fragaria_vesca FvH4_2g10250 FvH4_3g20400 FvH4_5g18050 FvH4_5g18070 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18470
malus_domestica MD06G1238100.v1.1 MD10G1022500.v1.1 MD10G1022600.v1.1 MD10G1022900.v1.1 MD10G1023100.v1.1 MD10G1023500.v1.1 MD14G1243000.v1.1 MD14G1243100.v1.1
prunus_persica Prupe.5G240700_v2.0.a1 Prupe.8G025500_v2.0.a1 Prupe.8G025600_v2.0.a1 Prupe.8G025700_v2.0.a1 Prupe.8G025800_v2.0.a1 Prupe.8G025900_v2.0.a1 Prupe.8G035600_v2.0.a1 Prupe.8G035700_v2.0.a1 Prupe.8G035800_v2.0.a1 Prupe.8G035900_v2.0.a1 Prupe.8G048300_v2.0.a1
pyrus_communis pycom06g21150 pycom06g21360 pycom06g21370 pycom14g09160 pycom14g09170 pycom14g20410 pycom14g20430 pycom14g20440
rosa_chinensis RchiOBHm_Chr1g0318151 RchiOBHm_Chr1g0354011 RchiOBHm_Chr1g0354031 RchiOBHm_Chr5g0034291 RchiOBHm_Chr7g0177551
rosa_laevigata RLG00000017504 RLG00000028234 RLG00000028236 RLG00000033537
rosa_multiflora Rmu_sc0004015.1_g000016 Rmu_sc0011424.1_g000001 Rmu_sc0011424.1_g000017 Rmu_sc0022466.1_g000003 Rmu_sc0033434.1_g000011 Rmu_ssc0000330.1_g000005 Rmu_ssc0000330.1_g000011
rosa_roxburghii Rroxscaffold_1G00045980 Rroxscaffold_2G00137150 Rroxscaffold_3G00275760 Rroxscaffold_4G00301290 Rroxscaffold_4G00301300 Rroxscaffold_4G00301320 Rroxscaffold_4G00301340 Rroxscaffold_4G00329570 Rroxscaffold_4G00329610 Rroxscaffold_4G00329760 Rroxscaffold_4G00329840 Rroxscaffold_4G00329920
rosa_rugosa Rorug01G0240100 Rorug01G0240200 Rorug01G0240500 Rorug06G0407200 Rorug06G0407300
rosa_samantha Rh1AG031100 Rh1AG251500 Rh1BG223100 Rh1CG029900 Rh1CG235700 Rh1CG235900 Rh1DG250000 Rh2BG197800 Rh5AG236600 Rh5BG237100 Rh5CG266900 Rh5DG244600 Rh7AG006500 Rh7BG006500 Rh7CG006900 Rh7CG007000 Rh7DG006500 Rh7DG006600
rosa_wichuraiana Rw1G002460 Rw1G002500 Rw1G021930 Rw2G014580 Rw7G000540 Rw7G000560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 309
AccII CGCG 1 cut(s) 306
AccIII TCCGGA 1 cut(s) 212
AciI CCGC 2 cut(s) 251, 335
AclWI GGATC 3 cut(s) 74, 80, 369
AcuI CTGAAG 1 cut(s) 77
AfaI GTAC 1 cut(s) 209
AgsI TTSAA 1 cut(s) 240
AleI CACNNNNGTG 1 cut(s) 351
AluBI AGCT 1 cut(s) 202
AluI AGCT 1 cut(s) 202
AlwI GGATC 3 cut(s) 74, 80, 369
Aor13HI TCCGGA 1 cut(s) 212
AoxI GGCC 1 cut(s) 325
ApeKI GCWGC 2 cut(s) 29, 32
AseI ATTAAT 1 cut(s) 114
AspS9I GGNCC 1 cut(s) 160
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 1 cut(s) 338
AvaII GGWCC 1 cut(s) 160
BauI CACGAG 1 cut(s) 351
BbvI GCAGC 2 cut(s) 16, 19
BceAI ACGGC 1 cut(s) 264
BcnI CCSGG 1 cut(s) 273
BisI GCNGC 3 cut(s) 30, 33, 252
BlsI GCNGC 3 cut(s) 31, 34, 253
Bme1390I CCNGG 1 cut(s) 273
Bme18I GGWCC 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 160
BmiI GGNNCC 1 cut(s) 162
BmrFI CCNGG 1 cut(s) 273
BmsI GCATC 1 cut(s) 182
BpuMI CCSGG 1 cut(s) 273
Bsa29I ATCGAT 1 cut(s) 364
BsaBI GATNNNNATC 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 357
BsaWI WCCGGW 3 cut(s) 204, 212, 229
Bse8I GATNNNNATC 1 cut(s) 84
BseAI TCCGGA 1 cut(s) 212
BseCI ATCGAT 1 cut(s) 364
BseDI CCNNGG 1 cut(s) 357
BseGI GGATG 1 cut(s) 268
BseJI GATNNNNATC 1 cut(s) 84
BseXI GCAGC 2 cut(s) 16, 19
Bsh1236I CGCG 1 cut(s) 306
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 1 cut(s) 327
BshVI ATCGAT 1 cut(s) 364
BsiEI CGRYCG 1 cut(s) 234
BsiSI CCGG 4 cut(s) 205, 213, 230, 273
BsmI GAATGC 1 cut(s) 50
BsnI GGCC 1 cut(s) 327
Bsp13I TCCGGA 1 cut(s) 212
Bsp143I GATC 4 cut(s) 79, 85, 361, 365
BspACI CCGC 2 cut(s) 251, 335
BspANI GGCC 1 cut(s) 327
BspDI ATCGAT 1 cut(s) 364
BspEI TCCGGA 1 cut(s) 212
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 1 cut(s) 162
BspPI GGATC 3 cut(s) 74, 80, 369
BssECI CCNNGG 1 cut(s) 357
BssMI GATC 4 cut(s) 79, 85, 361, 365
BssSI CACGAG 1 cut(s) 351
Bst2BI CACGAG 1 cut(s) 351
Bst6I CTCTTC 1 cut(s) 96
BstC8I GCNNGC 1 cut(s) 304
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 1 cut(s) 268
BstFNI CGCG 1 cut(s) 306
BstKTI GATC 4 cut(s) 82, 88, 364, 368
BstMBI GATC 4 cut(s) 79, 85, 361, 365
BstMCI CGRYCG 1 cut(s) 234
BstSCI CCNGG 1 cut(s) 271
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 2 cut(s) 16, 19
Bsu15I ATCGAT 1 cut(s) 364
BsuRI GGCC 1 cut(s) 327
BsuTUI ATCGAT 1 cut(s) 364
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 1 cut(s) 268
Cac8I GCNNGC 1 cut(s) 304
Cfr13I GGNCC 1 cut(s) 160
ClaI ATCGAT 1 cut(s) 364
Csp6I GTAC 1 cut(s) 208
CviJI RGCY 2 cut(s) 202, 327
CviKI_1 RGCY 2 cut(s) 202, 327
CviQI GTAC 1 cut(s) 208
DpnI GATC 4 cut(s) 81, 87, 363, 367
DpnII GATC 4 cut(s) 79, 85, 361, 365
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
EciI GGCGGA 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 77
EcoO109I RGGNCCY 1 cut(s) 160
FaiI YATR 3 cut(s) 269, 293, 373
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 3 cut(s) 30, 33, 252
FokI GGATG 1 cut(s) 275
Fsp4HI GCNGC 3 cut(s) 30, 33, 252
GluI GCNGC 3 cut(s) 30, 33, 252
HaeIII GGCC 1 cut(s) 327
HapII CCGG 4 cut(s) 205, 213, 230, 273
HinfI GANTC 2 cut(s) 226, 276
HpaII CCGG 4 cut(s) 205, 213, 230, 273
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 2 cut(s) 142, 310
Hpy188I TCNGA 2 cut(s) 79, 196
Hpy188III TCNNGA 3 cut(s) 83, 95, 213
Hpy8I GTNNAC 2 cut(s) 142, 310
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 2 cut(s) 156, 173
HpySE526I ACGT 1 cut(s) 258
Kpn2I TCCGGA 1 cut(s) 212
Kzo9I GATC 4 cut(s) 79, 85, 361, 365
LmnI GCTCC 1 cut(s) 207
LpnPI CCDG 7 cut(s) 33, 50, 80, 218, 226, 243, 286
Lsp1109I GCAGC 2 cut(s) 16, 19
LweI GCATC 1 cut(s) 182
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 2 cut(s) 22, 244
MalI GATC 4 cut(s) 81, 87, 363, 367
MboI GATC 4 cut(s) 79, 85, 361, 365
MboII GAAGA 3 cut(s) 83, 181, 360
MfeI CAATTG 1 cut(s) 240
MluCI AATT 2 cut(s) 111, 240
MlyI GAGTC 1 cut(s) 270
MnlI CCTC 7 cut(s) 71, 79, 85, 99, 338, 348, 360
MroI TCCGGA 1 cut(s) 212
MseI TTAA 1 cut(s) 114
MslI CAYNNNNRTG 1 cut(s) 351
MspI CCGG 4 cut(s) 205, 213, 230, 273
MspR9I CCNGG 1 cut(s) 273
MunI CAATTG 1 cut(s) 240
Mva1269I GAATGC 1 cut(s) 50
MvnI CGCG 1 cut(s) 306
NciI CCSGG 1 cut(s) 273
NdeII GATC 4 cut(s) 79, 85, 361, 365
NlaIV GGNNCC 1 cut(s) 162
NmeAIII GCCGAG 1 cut(s) 267
OliI CACNNNNGTG 1 cut(s) 351
PctI GAATGC 1 cut(s) 50
PfeI GAWTC 1 cut(s) 226
PkrI GCNGC 3 cut(s) 31, 34, 253
PleI GAGTC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 270
PpuMI RGGWCCY 1 cut(s) 160
PshBI ATTAAT 1 cut(s) 114
Psp5II RGGWCCY 1 cut(s) 160
PspN4I GGNNCC 1 cut(s) 162
PspPI GGNCC 1 cut(s) 160
PspPPI RGGWCCY 1 cut(s) 160
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 351
SaqAI TTAA 1 cut(s) 114
SatI GCNGC 3 cut(s) 30, 33, 252
Sau3AI GATC 4 cut(s) 79, 85, 361, 365
Sau96I GGNCC 1 cut(s) 160
SchI GAGTC 1 cut(s) 270
ScrFI CCNGG 1 cut(s) 273
SetI ASST 2 cut(s) 204, 261
SfaNI GCATC 1 cut(s) 182
SinI GGWCC 1 cut(s) 160
SmiMI CAYNNNNRTG 1 cut(s) 351
Sse9I AATT 2 cut(s) 111, 240
SsiI CCGC 2 cut(s) 251, 335
StyD4I CCNGG 1 cut(s) 271
TaiI ACGT 1 cut(s) 261
TaqI TCGA 2 cut(s) 224, 364
TasI AATT 2 cut(s) 111, 240
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 1 cut(s) 226
Tru1I TTAA 1 cut(s) 114
Tru9I TTAA 1 cut(s) 114
TseI GCWGC 2 cut(s) 29, 32
TspDTI ATGAA 2 cut(s) 17, 360
TspGWI ACGGA 1 cut(s) 374
VpaK11BI GGWCC 1 cut(s) 160
VspI ATTAAT 1 cut(s) 114
XmiI GTMKAC 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.