Rroxscaffold_4G00329840

Germin-like protein subfamily 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000366
Physical Location & Seq
Forward (+)
63217117 .. 63217940
824 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00329840.1

Sequence Viewer

Length: 696 bp
ATGAAAGGTGCTCATTGCCCTACAGCTGCTGCTTTTGCCCTATTGGCATTGGCAACCTTCCACCTTGTCTCTGCCTTTGATCCTAGTCCTCTCCAAGATTTCTGTGTAGCACTTAACACCACCAACACTGATATTTCTGCTGTGTTTGTGAATGGGAGATTCTGCAAGGACCCAAAGATGGTAACACCAGAGGATTTCTTCTTTTCCGGGCTCCGGATGCGCGGAAACACATCAAATCCGCTTGGTTCCATCGTCACCCAGGCCAATGTGCAGCAAATAGCAGGACTGAACACTCTTGGGGTATCCCTCGCTCGCATAGACTATGCACTTGGTGGACTAAACCCTCCTCACATCCACCCTCGGGCCACGGAAGTCCTTATGGTCATGGAAGGCACTCTCTACGTTGGCTTCGTCACATCCAATGATAACGGCAACCGCCTCTTCAGCAAAGTGTTGTACAAGGGAGATGTGTTTGTCTTCCCATTTGCTCAAATTCACTTCCAGCTTAACATCGGAAAGGTCAACGCCGTAGCCATTTCCAGTTTTAGCAGCCAGTTTCCTGGTGTCACCACCATAGCCAATGCAGTGTTCGGCTCCAACCCGTCAATCAACCCTGATGTTCTCACCACCGCCTTCCAAGTTGACAAAAATGTCGTTCAGTATCTCCAGAACCAGTTCTGGTATGACAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

231

Amino Acids

25.06

Weight (kDa)

6.49

Isoelectric Point (pI)

22.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 70 - 220 1.9e-48 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05950 AT5G38930 AT5G38940 AT5G38940 AT5G39130 AT5G39160 AT5G39160 AT5G39160 AT5G39190 AT5G39190
fragaria_vesca FvH4_2g10250 FvH4_3g20400 FvH4_5g18050 FvH4_5g18070 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18470
malus_domestica MD06G1238100.v1.1 MD10G1022500.v1.1 MD10G1022600.v1.1 MD10G1022900.v1.1 MD10G1023100.v1.1 MD10G1023500.v1.1 MD14G1243000.v1.1 MD14G1243100.v1.1
prunus_persica Prupe.5G240700_v2.0.a1 Prupe.8G025500_v2.0.a1 Prupe.8G025600_v2.0.a1 Prupe.8G025700_v2.0.a1 Prupe.8G025800_v2.0.a1 Prupe.8G025900_v2.0.a1 Prupe.8G035600_v2.0.a1 Prupe.8G035700_v2.0.a1 Prupe.8G035800_v2.0.a1 Prupe.8G035900_v2.0.a1 Prupe.8G048300_v2.0.a1
pyrus_communis pycom06g21150 pycom06g21360 pycom06g21370 pycom14g09160 pycom14g09170 pycom14g20410 pycom14g20430 pycom14g20440
rosa_chinensis RchiOBHm_Chr1g0318151 RchiOBHm_Chr1g0354011 RchiOBHm_Chr1g0354031 RchiOBHm_Chr5g0034291 RchiOBHm_Chr7g0177551
rosa_laevigata RLG00000017504 RLG00000028234 RLG00000028236 RLG00000033537
rosa_multiflora Rmu_sc0004015.1_g000016 Rmu_sc0011424.1_g000001 Rmu_sc0011424.1_g000017 Rmu_sc0022466.1_g000003 Rmu_sc0033434.1_g000011 Rmu_ssc0000330.1_g000005 Rmu_ssc0000330.1_g000011
rosa_roxburghii Rroxscaffold_1G00045980 Rroxscaffold_2G00137150 Rroxscaffold_3G00275760 Rroxscaffold_4G00301290 Rroxscaffold_4G00301300 Rroxscaffold_4G00301320 Rroxscaffold_4G00301340 Rroxscaffold_4G00329570 Rroxscaffold_4G00329610 Rroxscaffold_4G00329760 Rroxscaffold_4G00329840 Rroxscaffold_4G00329920
rosa_rugosa Rorug01G0240100 Rorug01G0240200 Rorug01G0240500 Rorug06G0407200 Rorug06G0407300
rosa_samantha Rh1AG031100 Rh1AG251500 Rh1BG223100 Rh1CG029900 Rh1CG235700 Rh1CG235900 Rh1DG250000 Rh2BG197800 Rh5AG236600 Rh5BG237100 Rh5CG266900 Rh5DG244600 Rh7AG006500 Rh7BG006500 Rh7CG006900 Rh7CG007000 Rh7DG006500 Rh7DG006600
rosa_wichuraiana Rw1G002460 Rw1G002500 Rw1G021930 Rw2G014580 Rw7G000540 Rw7G000560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 650
AccII CGCG 1 cut(s) 222
AccIII TCCGGA 1 cut(s) 213
AciI CCGC 4 cut(s) 222, 239, 436, 630
AclWI GGATC 1 cut(s) 74
AcsI RAATTY 1 cut(s) 492
AcuI CTGAAG 1 cut(s) 427
AdeI CACNNNGTG 1 cut(s) 332
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 3 cut(s) 178, 213, 361
AjnI CCWGG 2 cut(s) 258, 559
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
AluBI AGCT 2 cut(s) 26, 505
AluI AGCT 2 cut(s) 26, 505
Alw21I GWGCWC 1 cut(s) 13
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 1 cut(s) 74
AlwNI CAGNNNCTG 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 360
Aor13HI TCCGGA 1 cut(s) 213
AoxI GGCC 2 cut(s) 261, 363
ApeKI GCWGC 4 cut(s) 26, 29, 271, 549
ApoI RAATTY 1 cut(s) 492
Asp700I GAANNNNTTC 1 cut(s) 674
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 2 cut(s) 169, 363
AsuC2I CCSGG 1 cut(s) 208
AsuHPI GGTGA 3 cut(s) 247, 559, 616
AvaI CYCGRG 1 cut(s) 360
AvaII GGWCC 1 cut(s) 169
BanII GRGCYC 1 cut(s) 213
BbsI GAAGAC 1 cut(s) 469
Bbv12I GWGCWC 1 cut(s) 13
BbvI GCAGC 4 cut(s) 13, 16, 283, 561
BccI CCATC 2 cut(s) 172, 257
BceAI ACGGC 2 cut(s) 445, 512
BciT130I CCWGG 2 cut(s) 260, 561
BciVI GTATCC 1 cut(s) 313
BcnI CCSGG 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 73
BfaI CTAG 1 cut(s) 84
BfmI CTRYAG 1 cut(s) 21
BfuI GTATCC 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 44
BisI GCNGC 4 cut(s) 27, 30, 272, 550
BlsI GCNGC 4 cut(s) 28, 31, 273, 551
Bme1390I CCNGG 3 cut(s) 208, 260, 561
Bme18I GGWCC 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 360
BmgT120I GGNCC 2 cut(s) 169, 363
BmiI GGNNCC 4 cut(s) 171, 212, 247, 595
BmrFI CCNGG 3 cut(s) 208, 260, 561
BmsI GCATC 1 cut(s) 207
BpiI GAAGAC 1 cut(s) 469
BpmI CTGGAG 1 cut(s) 650
BpuMI CCSGG 1 cut(s) 208
BsaJI CCNNGG 3 cut(s) 258, 359, 366
BsaWI WCCGGW 1 cut(s) 213
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 213, 361
Bse1I ACTGG 3 cut(s) 540, 553, 673
Bse3DI GCAATG 1 cut(s) 13
BseAI TCCGGA 1 cut(s) 213
BseBI CCWGG 2 cut(s) 260, 561
BseDI CCNNGG 3 cut(s) 258, 359, 366
BseGI GGATG 3 cut(s) 222, 351, 416
BseLI CCNNNNNNNGG 3 cut(s) 178, 213, 361
BseMI GCAATG 1 cut(s) 13
BseNI ACTGG 3 cut(s) 540, 553, 673
BseRI GAGGAG 1 cut(s) 336
BseXI GCAGC 4 cut(s) 13, 16, 283, 561
BsgI GTGCAG 1 cut(s) 290
Bsh1236I CGCG 1 cut(s) 222
BshFI GGCC 2 cut(s) 263, 365
BsiHKAI GWGCWC 1 cut(s) 13
BsiHKCI CYCGRG 1 cut(s) 360
BsiSI CCGG 2 cut(s) 207, 214
BslI CCNNNNNNNGG 3 cut(s) 178, 213, 361
BsmAI GTCTC 1 cut(s) 73
BsnI GGCC 2 cut(s) 263, 365
BsoBI CYCGRG 1 cut(s) 360
Bsp1286I GDGCHC 2 cut(s) 13, 213
Bsp13I TCCGGA 1 cut(s) 213
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 1 cut(s) 79
BspACI CCGC 4 cut(s) 222, 239, 436, 630
BspANI GGCC 2 cut(s) 263, 365
BspEI TCCGGA 1 cut(s) 213
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 4 cut(s) 171, 212, 247, 595
BspPI GGATC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 13
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 3 cut(s) 540, 553, 673
BssECI CCNNGG 3 cut(s) 258, 359, 366
BssMI GATC 1 cut(s) 79
Bst2UI CCWGG 2 cut(s) 260, 561
Bst6I CTCTTC 1 cut(s) 446
BstAUI TGTACA 1 cut(s) 456
BstC8I GCNNGC 1 cut(s) 313
BstDSI CCRYGG 1 cut(s) 366
BstF5I GGATG 3 cut(s) 222, 351, 416
BstFNI CGCG 1 cut(s) 222
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 1 cut(s) 82
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 1 cut(s) 79
BstMWI GCNNNNNNNGC 4 cut(s) 35, 44, 217, 444
BstNI CCWGG 2 cut(s) 260, 561
BstSCI CCNGG 3 cut(s) 206, 258, 559
BstSFI CTRYAG 1 cut(s) 21
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 4 cut(s) 13, 16, 283, 561
BstV2I GAAGAC 1 cut(s) 469
BstXI CCANNNNNNTGG 1 cut(s) 560
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 2 cut(s) 263, 365
BtgI CCRYGG 1 cut(s) 366
BtsCI GGATG 3 cut(s) 222, 351, 416
BtsI GCAGTG 1 cut(s) 591
BtsIMutI CAGTG 2 cut(s) 126, 591
Cac8I GCNNGC 1 cut(s) 313
CaiI CAGNNNCTG 1 cut(s) 29
CfoI GCGC 1 cut(s) 222
Cfr13I GGNCC 2 cut(s) 169, 363
Csp6I GTAC 1 cut(s) 457
CviAII CATG 1 cut(s) 385
CviQI GTAC 1 cut(s) 457
DpnI GATC 1 cut(s) 81
DpnII GATC 1 cut(s) 79
DraIII CACNNNGTG 1 cut(s) 332
DrdI GACNNNNNNGTC 1 cut(s) 650
DseDI GACNNNNNNGTC 1 cut(s) 650
Eam1104I CTCTTC 1 cut(s) 446
EarI CTCTTC 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 213
Eco47I GGWCC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 427
Eco88I CYCGRG 1 cut(s) 360
EcoO109I RGGNCCY 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 258, 559
EcoT38I GRGCYC 1 cut(s) 213
FaeI CATG 1 cut(s) 388
FaiI YATR 6 cut(s) 317, 324, 380, 386, 575, 684
FatI CATG 1 cut(s) 384
Fnu4HI GCNGC 4 cut(s) 27, 30, 272, 550
FokI GGATG 3 cut(s) 229, 338, 403
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 4 cut(s) 27, 30, 272, 550
FspBI CTAG 1 cut(s) 84
GlaI GCGC 1 cut(s) 221
GluI GCNGC 4 cut(s) 27, 30, 272, 550
GsuI CTGGAG 1 cut(s) 650
HaeIII GGCC 2 cut(s) 263, 365
HapII CCGG 2 cut(s) 207, 214
HhaI GCGC 1 cut(s) 222
Hin1II CATG 1 cut(s) 388
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HincII GTYRAC 2 cut(s) 523, 643
HindII GTYRAC 2 cut(s) 523, 643
HinfI GANTC 1 cut(s) 159
HpaII CCGG 2 cut(s) 207, 214
HphI GGTGA 3 cut(s) 247, 559, 616
Hpy166II GTNNAC 3 cut(s) 335, 523, 643
Hpy188I TCNGA 1 cut(s) 515
Hpy188III TCNNGA 2 cut(s) 214, 667
Hpy8I GTNNAC 3 cut(s) 335, 523, 643
HpyAV CCTTC 3 cut(s) 67, 383, 643
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 4 cut(s) 165, 271, 326, 584
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 44, 217, 444
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 1 cut(s) 388
HspAI GCGC 1 cut(s) 220
Kpn2I TCCGGA 1 cut(s) 213
Kzo9I GATC 1 cut(s) 79
LmnI GCTCC 2 cut(s) 216, 599
Lsp1109I GCAGC 4 cut(s) 13, 16, 283, 561
LweI GCATC 1 cut(s) 207
MaeI CTAG 1 cut(s) 84
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 4 cut(s) 181, 253, 412, 565
MalI GATC 1 cut(s) 81
MboI GATC 1 cut(s) 79
MboII GAAGA 3 cut(s) 190, 433, 469
MhlI GDGCHC 2 cut(s) 13, 213
MluCI AATT 2 cut(s) 492, 691
MmeI TCCRAC 1 cut(s) 621
MnlI CCTC 7 cut(s) 99, 184, 317, 354, 357, 369, 449
MroI TCCGGA 1 cut(s) 213
MroXI GAANNNNTTC 1 cut(s) 674
MseI TTAA 3 cut(s) 114, 507, 694
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 2 cut(s) 207, 214
MspR9I CCNGG 3 cut(s) 208, 260, 561
MvaI CCWGG 2 cut(s) 260, 561
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 4 cut(s) 35, 44, 217, 444
NciI CCSGG 1 cut(s) 208
NdeII GATC 1 cut(s) 79
NlaIII CATG 1 cut(s) 388
NlaIV GGNNCC 4 cut(s) 171, 212, 247, 595
NmuCI GTSAC 3 cut(s) 253, 412, 565
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 674
PfeI GAWTC 1 cut(s) 159
PkrI GCNGC 4 cut(s) 28, 31, 273, 551
PpuMI RGGWCCY 1 cut(s) 169
Psp5II RGGWCCY 1 cut(s) 169
Psp6I CCWGG 2 cut(s) 258, 559
PspGI CCWGG 2 cut(s) 258, 559
PspN4I GGNNCC 4 cut(s) 171, 212, 247, 595
PspPI GGNCC 2 cut(s) 169, 363
PspPPI RGGWCCY 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
SaqAI TTAA 3 cut(s) 114, 507, 694
SatI GCNGC 4 cut(s) 27, 30, 272, 550
Sau3AI GATC 1 cut(s) 79
Sau96I GGNCC 2 cut(s) 169, 363
ScrFI CCNGG 3 cut(s) 208, 260, 561
SduI GDGCHC 2 cut(s) 13, 213
SetI ASST 7 cut(s) 10, 28, 59, 66, 405, 507, 522
SfaNI GCATC 1 cut(s) 207
SfcI CTRYAG 1 cut(s) 21
SinI GGWCC 1 cut(s) 169
Sse9I AATT 2 cut(s) 492, 691
SsiI CCGC 4 cut(s) 222, 239, 436, 630
SspMI CTAG 1 cut(s) 84
StyD4I CCNGG 3 cut(s) 206, 258, 559
TaiI ACGT 1 cut(s) 405
TasI AATT 2 cut(s) 492, 691
TatI WGTACW 1 cut(s) 456
TfiI GAWTC 1 cut(s) 159
Tru1I TTAA 3 cut(s) 114, 507, 694
Tru9I TTAA 3 cut(s) 114, 507, 694
TscAI CASTG 2 cut(s) 133, 591
TseFI GTSAC 3 cut(s) 253, 412, 565
TseI GCWGC 4 cut(s) 26, 29, 271, 549
Tsp45I GTSAC 3 cut(s) 253, 412, 565
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 383
TspRI CASTG 2 cut(s) 133, 591
VpaK11BI GGWCC 1 cut(s) 169
XapI RAATTY 1 cut(s) 492
XmnI GAANNNNTTC 1 cut(s) 674
XspI CTAG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.