Rroxscaffold_2G00137150

Germin-like protein subfamily 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000366
Physical Location & Seq
Reverse (-)
74849023 .. 74849899
877 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00137150.1

Sequence Viewer

Length: 702 bp
ATGATGATGAAAGGTGCTCATTTCCCTACAGCTGCTGCTTTTTCCCTATTGGCATTAGCAACCTTCCACCTTTTATCTGCCTCTGATCCTAGTCCTCTTCAAGATTTCTGTGTAGCACTTAACACCGCCCAAACTGCTGTTTCTGCTGTGTTTGTGAATGGGAAATTCTGCAAGGACCCAAAGCTTGCAACAGCAAATGATTTCTTCTTTTCCGGGCTCCAGATTCTCCGAAACACATCAAATCCGGTTGGTTCTAACGTCACCGCAGTTAATGTGGAGCAAATAGCAGGGCTGAACACTCTTGGGATATCCCTGGCTCGCATAGACTATGCACCAAATGGCGGACTAAACCCTCCTCACACCCACCCTCGGGCCACGGAAATTCTTGTAGTCATGGAAGGCACTCTATACGTTGGCTTCGTCACATCTAACCCTGACAACCGCCTCTTCACCAAAGTGTTGAGCAAGGGAGATGTGTTTGTCTTCCCAGTTGGTCTCATTCACTTCCAATTCAACATCGGAGAGGTTAACGCCGTAGCCATTGCCGCTCTTAGCAGCCAGAATCCAGGTGTCATCACCATAGCCAATGCAGTTTTCGGCTCCAACCCTCCAATCAACCCTGATGTTCTCAGCAAGGCCTTCCAAGTTGACAACAATATCGTTCAGTATCTCCAGAAACAGTTCTGGTACAACAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

233

Amino Acids

24.97

Weight (kDa)

6.49

Isoelectric Point (pI)

23.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 76 - 221 9e-54 Cupin
Cupin_2 PF07883 108 - 181 3e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05950 AT5G38930 AT5G38940 AT5G38940 AT5G39130 AT5G39160 AT5G39160 AT5G39160 AT5G39190 AT5G39190
fragaria_vesca FvH4_2g10250 FvH4_3g20400 FvH4_5g18050 FvH4_5g18070 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18470
malus_domestica MD06G1238100.v1.1 MD10G1022500.v1.1 MD10G1022600.v1.1 MD10G1022900.v1.1 MD10G1023100.v1.1 MD10G1023500.v1.1 MD14G1243000.v1.1 MD14G1243100.v1.1
prunus_persica Prupe.5G240700_v2.0.a1 Prupe.8G025500_v2.0.a1 Prupe.8G025600_v2.0.a1 Prupe.8G025700_v2.0.a1 Prupe.8G025800_v2.0.a1 Prupe.8G025900_v2.0.a1 Prupe.8G035600_v2.0.a1 Prupe.8G035700_v2.0.a1 Prupe.8G035800_v2.0.a1 Prupe.8G035900_v2.0.a1 Prupe.8G048300_v2.0.a1
pyrus_communis pycom06g21150 pycom06g21360 pycom06g21370 pycom14g09160 pycom14g09170 pycom14g20410 pycom14g20430 pycom14g20440
rosa_chinensis RchiOBHm_Chr1g0318151 RchiOBHm_Chr1g0354011 RchiOBHm_Chr1g0354031 RchiOBHm_Chr5g0034291 RchiOBHm_Chr7g0177551
rosa_laevigata RLG00000017504 RLG00000028234 RLG00000028236 RLG00000033537
rosa_multiflora Rmu_sc0004015.1_g000016 Rmu_sc0011424.1_g000001 Rmu_sc0011424.1_g000017 Rmu_sc0022466.1_g000003 Rmu_sc0033434.1_g000011 Rmu_ssc0000330.1_g000005 Rmu_ssc0000330.1_g000011
rosa_roxburghii Rroxscaffold_1G00045980 Rroxscaffold_2G00137150 Rroxscaffold_3G00275760 Rroxscaffold_4G00301290 Rroxscaffold_4G00301300 Rroxscaffold_4G00301320 Rroxscaffold_4G00301340 Rroxscaffold_4G00329570 Rroxscaffold_4G00329610 Rroxscaffold_4G00329760 Rroxscaffold_4G00329840 Rroxscaffold_4G00329920
rosa_rugosa Rorug01G0240100 Rorug01G0240200 Rorug01G0240500 Rorug06G0407200 Rorug06G0407300
rosa_samantha Rh1AG031100 Rh1AG251500 Rh1BG223100 Rh1CG029900 Rh1CG235700 Rh1CG235900 Rh1DG250000 Rh2BG197800 Rh5AG236600 Rh5BG237100 Rh5CG266900 Rh5DG244600 Rh7AG006500 Rh7BG006500 Rh7CG006900 Rh7CG007000 Rh7DG006500 Rh7DG006600
rosa_wichuraiana Rw1G002460 Rw1G002500 Rw1G021930 Rw2G014580 Rw7G000540 Rw7G000560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 548
AciI CCGC 5 cut(s) 126, 264, 342, 442, 546
AclWI GGATC 1 cut(s) 80
AcsI RAATTY 2 cut(s) 164, 381
AfaI GTAC 1 cut(s) 689
AfiI CCNNNNNNNGG 3 cut(s) 341, 369, 370
AgsI TTSAA 2 cut(s) 101, 514
AjnI CCWGG 2 cut(s) 312, 565
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
AleI CACNNNNGTG 1 cut(s) 455
AluBI AGCT 2 cut(s) 32, 184
AluI AGCT 2 cut(s) 32, 184
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 1 cut(s) 80
AlwNI CAGNNNCTG 1 cut(s) 35
Ama87I CYCGRG 1 cut(s) 369
AoxI GGCC 2 cut(s) 372, 636
ApeKI GCWGC 3 cut(s) 32, 35, 555
ApoI RAATTY 2 cut(s) 164, 381
Asp700I GAANNNNTTC 1 cut(s) 680
AspS9I GGNCC 2 cut(s) 175, 372
AsuC2I CCSGG 1 cut(s) 214
AsuHPI GGTGA 3 cut(s) 253, 442, 568
AvaI CYCGRG 1 cut(s) 369
AvaII GGWCC 1 cut(s) 175
BanII GRGCYC 1 cut(s) 219
BbsI GAAGAC 1 cut(s) 475
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 3 cut(s) 19, 22, 567
BceAI ACGGC 1 cut(s) 518
BciT130I CCWGG 2 cut(s) 314, 567
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 1 cut(s) 27
BisI GCNGC 4 cut(s) 33, 36, 546, 556
BlsI GCNGC 4 cut(s) 34, 37, 547, 557
Bme1390I CCNGG 3 cut(s) 214, 314, 567
Bme18I GGWCC 1 cut(s) 175
BmeT110I CYCGRG 1 cut(s) 369
BmgT120I GGNCC 2 cut(s) 175, 372
BmiI GGNNCC 3 cut(s) 177, 218, 601
BmrFI CCNGG 3 cut(s) 214, 314, 567
BmrI ACTGGG 1 cut(s) 482
BmuI ACTGGG 1 cut(s) 482
BpiI GAAGAC 1 cut(s) 475
BpmI CTGGAG 2 cut(s) 203, 656
BpuMI CCSGG 1 cut(s) 214
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 3 cut(s) 312, 368, 375
BsaWI WCCGGW 1 cut(s) 244
Bsc4I CCNNNNNNNGG 3 cut(s) 341, 369, 370
Bse1I ACTGG 1 cut(s) 488
Bse3DI GCAATG 1 cut(s) 540
BseBI CCWGG 2 cut(s) 314, 567
BseDI CCNNGG 3 cut(s) 312, 368, 375
BseLI CCNNNNNNNGG 3 cut(s) 341, 369, 370
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 1 cut(s) 643
BseNI ACTGG 1 cut(s) 488
BseRI GAGGAG 1 cut(s) 345
BseXI GCAGC 3 cut(s) 19, 22, 567
BshFI GGCC 2 cut(s) 374, 638
BsiHKAI GWGCWC 1 cut(s) 19
BsiHKCI CYCGRG 1 cut(s) 369
BsiSI CCGG 2 cut(s) 213, 245
BslI CCNNNNNNNGG 3 cut(s) 341, 369, 370
BsmAI GTCTC 1 cut(s) 500
BsnI GGCC 2 cut(s) 374, 638
Bso31I GGTCTC 1 cut(s) 500
BsoBI CYCGRG 1 cut(s) 369
Bsp1286I GDGCHC 2 cut(s) 19, 219
Bsp143I GATC 1 cut(s) 85
BspACI CCGC 5 cut(s) 126, 264, 342, 442, 546
BspANI GGCC 2 cut(s) 374, 638
BspCNI CTCAG 1 cut(s) 642
BspLI GGNNCC 3 cut(s) 177, 218, 601
BspPI GGATC 1 cut(s) 80
BspTNI GGTCTC 1 cut(s) 500
BsrBI CCGCTC 1 cut(s) 548
BsrDI GCAATG 1 cut(s) 540
BsrI ACTGG 1 cut(s) 488
BssECI CCNNGG 3 cut(s) 312, 368, 375
BssMI GATC 1 cut(s) 85
Bst2UI CCWGG 2 cut(s) 314, 567
Bst4CI ACNGT 1 cut(s) 681
Bst6I CTCTTC 2 cut(s) 102, 452
BstC8I GCNNGC 2 cut(s) 186, 319
BstDEI CTNAG 2 cut(s) 551, 629
BstDSI CCRYGG 1 cut(s) 375
BstKTI GATC 1 cut(s) 88
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 1 cut(s) 85
BstMWI GCNNNNNNNGC 3 cut(s) 134, 143, 545
BstNI CCWGG 2 cut(s) 314, 567
BstSCI CCNGG 3 cut(s) 212, 312, 565
BstSFI CTRYAG 1 cut(s) 27
BstV1I GCAGC 3 cut(s) 19, 22, 567
BstV2I GAAGAC 1 cut(s) 475
BsuRI GGCC 2 cut(s) 374, 638
BtgI CCRYGG 1 cut(s) 375
Cac8I GCNNGC 2 cut(s) 186, 319
CaiI CAGNNNCTG 1 cut(s) 35
Cfr13I GGNCC 2 cut(s) 175, 372
Csp6I GTAC 1 cut(s) 688
CviAII CATG 1 cut(s) 394
CviQI GTAC 1 cut(s) 688
DdeI CTNAG 2 cut(s) 551, 629
DpnI GATC 1 cut(s) 87
DpnII GATC 1 cut(s) 85
Eam1104I CTCTTC 2 cut(s) 102, 452
EarI CTCTTC 2 cut(s) 102, 452
EciI GGCGGA 1 cut(s) 357
Eco147I AGGCCT 1 cut(s) 638
Eco24I GRGCYC 1 cut(s) 219
Eco31I GGTCTC 1 cut(s) 500
Eco32I GATATC 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 175
Eco88I CYCGRG 1 cut(s) 369
EcoO109I RGGNCCY 1 cut(s) 175
EcoRII CCWGG 2 cut(s) 312, 565
EcoRV GATATC 1 cut(s) 309
EcoT38I GRGCYC 1 cut(s) 219
FaeI CATG 1 cut(s) 397
FaiI YATR 5 cut(s) 323, 330, 395, 409, 581
FatI CATG 1 cut(s) 393
Fnu4HI GCNGC 4 cut(s) 33, 36, 546, 556
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 4 cut(s) 33, 36, 546, 556
FspBI CTAG 1 cut(s) 90
GluI GCNGC 4 cut(s) 33, 36, 546, 556
GsuI CTGGAG 2 cut(s) 203, 656
HaeIII GGCC 2 cut(s) 374, 638
HapII CCGG 2 cut(s) 213, 245
Hin1II CATG 1 cut(s) 397
HincII GTYRAC 2 cut(s) 529, 649
HindII GTYRAC 2 cut(s) 529, 649
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 2 cut(s) 223, 562
HpaI GTTAAC 1 cut(s) 529
HpaII CCGG 2 cut(s) 213, 245
HphI GGTGA 3 cut(s) 253, 442, 568
Hpy166II GTNNAC 2 cut(s) 529, 649
Hpy188I TCNGA 3 cut(s) 85, 230, 521
Hpy188III TCNNGA 3 cut(s) 101, 220, 673
Hpy8I GTNNAC 2 cut(s) 529, 649
HpyAV CCTTC 3 cut(s) 73, 392, 649
HpyCH4III ACNGT 1 cut(s) 681
HpyCH4IV ACGT 2 cut(s) 258, 411
HpyCH4V TGCA 4 cut(s) 171, 188, 332, 590
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 143, 545
HpyF3I CTNAG 2 cut(s) 551, 629
HpySE526I ACGT 2 cut(s) 258, 411
Hsp92II CATG 1 cut(s) 397
KspAI GTTAAC 1 cut(s) 529
Kzo9I GATC 1 cut(s) 85
LmnI GCTCC 3 cut(s) 222, 277, 605
Lsp1109I GCAGC 3 cut(s) 19, 22, 567
MaeI CTAG 1 cut(s) 90
MaeII ACGT 2 cut(s) 258, 411
MaeIII GTNAC 2 cut(s) 259, 421
MalI GATC 1 cut(s) 87
MbiI CCGCTC 1 cut(s) 548
MboI GATC 1 cut(s) 85
MboII GAAGA 4 cut(s) 89, 196, 439, 475
MhlI GDGCHC 2 cut(s) 19, 219
MluCI AATT 4 cut(s) 164, 381, 509, 697
MmeI TCCRAC 1 cut(s) 627
MnlI CCTC 8 cut(s) 91, 105, 363, 366, 378, 455, 517, 618
MroXI GAANNNNTTC 1 cut(s) 680
MseI TTAA 4 cut(s) 120, 270, 528, 700
MslI CAYNNNNRTG 1 cut(s) 455
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 2 cut(s) 213, 245
MspR9I CCNGG 3 cut(s) 214, 314, 567
MvaI CCWGG 2 cut(s) 314, 567
MwoI GCNNNNNNNGC 3 cut(s) 134, 143, 545
NciI CCSGG 1 cut(s) 214
NdeII GATC 1 cut(s) 85
NlaIII CATG 1 cut(s) 397
NlaIV GGNNCC 3 cut(s) 177, 218, 601
NmuCI GTSAC 2 cut(s) 259, 421
OliI CACNNNNGTG 1 cut(s) 455
PceI AGGCCT 1 cut(s) 638
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PdmI GAANNNNTTC 1 cut(s) 680
PfeI GAWTC 2 cut(s) 223, 562
PkrI GCNGC 4 cut(s) 34, 37, 547, 557
PpuMI RGGWCCY 1 cut(s) 175
Psp5II RGGWCCY 1 cut(s) 175
Psp6I CCWGG 2 cut(s) 312, 565
PspGI CCWGG 2 cut(s) 312, 565
PspN4I GGNNCC 3 cut(s) 177, 218, 601
PspPI GGNCC 2 cut(s) 175, 372
PspPPI RGGWCCY 1 cut(s) 175
PstNI CAGNNNCTG 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 1 cut(s) 689
RsaNI GTAC 1 cut(s) 688
RseI CAYNNNNRTG 1 cut(s) 455
SaqAI TTAA 4 cut(s) 120, 270, 528, 700
SatI GCNGC 4 cut(s) 33, 36, 546, 556
Sau3AI GATC 1 cut(s) 85
Sau96I GGNCC 2 cut(s) 175, 372
ScrFI CCNGG 3 cut(s) 214, 314, 567
SduI GDGCHC 2 cut(s) 19, 219
SetI ASST 9 cut(s) 16, 34, 65, 72, 186, 261, 414, 528, 571
SfcI CTRYAG 1 cut(s) 27
SinI GGWCC 1 cut(s) 175
SmiMI CAYNNNNRTG 1 cut(s) 455
Sse9I AATT 4 cut(s) 164, 381, 509, 697
SseBI AGGCCT 1 cut(s) 638
SsiI CCGC 5 cut(s) 126, 264, 342, 442, 546
SspMI CTAG 1 cut(s) 90
StuI AGGCCT 1 cut(s) 638
StyD4I CCNGG 3 cut(s) 212, 312, 565
TaaI ACNGT 1 cut(s) 681
TaiI ACGT 2 cut(s) 261, 414
TasI AATT 4 cut(s) 164, 381, 509, 697
TauI GCSGC 1 cut(s) 548
TfiI GAWTC 2 cut(s) 223, 562
Tru1I TTAA 4 cut(s) 120, 270, 528, 700
Tru9I TTAA 4 cut(s) 120, 270, 528, 700
TseFI GTSAC 2 cut(s) 259, 421
TseI GCWGC 3 cut(s) 32, 35, 555
Tsp45I GTSAC 2 cut(s) 259, 421
TspDTI ATGAA 1 cut(s) 23
TspGWI ACGGA 1 cut(s) 392
VpaK11BI GGWCC 1 cut(s) 175
XapI RAATTY 2 cut(s) 164, 381
XmnI GAANNNNTTC 1 cut(s) 680
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.