MD10G1022900.v1.1

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000366
Physical Location & Seq
Reverse (-)
2873448 .. 2874602
1155 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1022900.v1.1.491

Sequence Viewer

Length: 681 bp
ATGAAAGGTGCTCAGTTCCTCGTATGCATTGTTGCCATCTTGGCATTCGCAACCTCCCTTGTCTCTAGCTCTGACCCCGGTCCCCTCCAGGACTTCTGCGTTGCAATTAATGACACCGAATTAGGGGTGTTTGTGAACGGGAAATTCTGCAAGGATCCACAGCTTGTATCAGCAGATGATTTCTCCTTATCTGGGCTTCAAATTCCAAGAAACACACAAAATCCGTTGGGTTCAAAGGTAACACTTGCTAATGTGGACCTAATAGCAGGACTAAACACTCTCGGTCTATCCCTAGGTCGCATAGACTATGCACCAAATGGCGGCCTAAACCCTCCCCACAGTCACCCTCGCGCCTCGGAACTCCTTTTTGTCCAGGAAGGTACACTCTACGTTGGGTTCGTAACGTCCAACCCGGATCATCGTCTATTTACCAAGGTGTTGAACAAGGGAGATGTGTTTGTGTTCCCAGTTGGTCTTATTCACTTCCAACTGAATGTGGGACATACCAGTGCTGTGGCCTTTGCTAGTTTTAGTAGCCAGAACCCAGGAGTCATCACCATAGCAAATGCATTGTTTGACGCCAACCCTTCCATCAATCCTGATGTTCTAGCCAAGGCCTTCCAACTCGATGACAAAATGGTTACATATCTTCAAAAACAGGTCTGGTACGAAAACACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

227

Amino Acids

24.28

Weight (kDa)

5.39

Isoelectric Point (pI)

18.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 65 - 214 3.4e-50 Cupin
Cupin_2 PF07883 101 - 174 5e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05950 AT5G38930 AT5G38940 AT5G38940 AT5G39130 AT5G39160 AT5G39160 AT5G39160 AT5G39190 AT5G39190
fragaria_vesca FvH4_2g10250 FvH4_3g20400 FvH4_5g18050 FvH4_5g18070 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18470
malus_domestica MD06G1238100.v1.1 MD10G1022500.v1.1 MD10G1022600.v1.1 MD10G1022900.v1.1 MD10G1023100.v1.1 MD10G1023500.v1.1 MD14G1243000.v1.1 MD14G1243100.v1.1
prunus_persica Prupe.5G240700_v2.0.a1 Prupe.8G025500_v2.0.a1 Prupe.8G025600_v2.0.a1 Prupe.8G025700_v2.0.a1 Prupe.8G025800_v2.0.a1 Prupe.8G025900_v2.0.a1 Prupe.8G035600_v2.0.a1 Prupe.8G035700_v2.0.a1 Prupe.8G035800_v2.0.a1 Prupe.8G035900_v2.0.a1 Prupe.8G048300_v2.0.a1
pyrus_communis pycom06g21150 pycom06g21360 pycom06g21370 pycom14g09160 pycom14g09170 pycom14g20410 pycom14g20430 pycom14g20440
rosa_chinensis RchiOBHm_Chr1g0318151 RchiOBHm_Chr1g0354011 RchiOBHm_Chr1g0354031 RchiOBHm_Chr5g0034291 RchiOBHm_Chr7g0177551
rosa_laevigata RLG00000017504 RLG00000028234 RLG00000028236 RLG00000033537
rosa_multiflora Rmu_sc0004015.1_g000016 Rmu_sc0011424.1_g000001 Rmu_sc0011424.1_g000017 Rmu_sc0022466.1_g000003 Rmu_sc0033434.1_g000011 Rmu_ssc0000330.1_g000005 Rmu_ssc0000330.1_g000011
rosa_roxburghii Rroxscaffold_1G00045980 Rroxscaffold_2G00137150 Rroxscaffold_3G00275760 Rroxscaffold_4G00301290 Rroxscaffold_4G00301300 Rroxscaffold_4G00301320 Rroxscaffold_4G00301340 Rroxscaffold_4G00329570 Rroxscaffold_4G00329610 Rroxscaffold_4G00329760 Rroxscaffold_4G00329840 Rroxscaffold_4G00329920
rosa_rugosa Rorug01G0240100 Rorug01G0240200 Rorug01G0240500 Rorug06G0407200 Rorug06G0407300
rosa_samantha Rh1AG031100 Rh1AG251500 Rh1BG223100 Rh1CG029900 Rh1CG235700 Rh1CG235900 Rh1DG250000 Rh2BG197800 Rh5AG236600 Rh5BG237100 Rh5CG266900 Rh5DG244600 Rh7AG006500 Rh7BG006500 Rh7CG006900 Rh7CG007000 Rh7DG006500 Rh7DG006600
rosa_wichuraiana Rw1G002460 Rw1G002500 Rw1G021930 Rw2G014580 Rw7G000540 Rw7G000560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 351
AciI CCGC 1 cut(s) 321
AclWI GGATC 3 cut(s) 149, 162, 423
AcsI RAATTY 2 cut(s) 143, 201
AcyI GRCGYC 1 cut(s) 579
AfaI GTAC 2 cut(s) 382, 668
AfiI CCNNNNNNNGG 3 cut(s) 123, 192, 320
AgsI TTSAA 4 cut(s) 200, 234, 442, 653
AjnI CCWGG 3 cut(s) 87, 372, 544
AluBI AGCT 2 cut(s) 69, 163
AluI AGCT 2 cut(s) 69, 163
Alw21I GWGCWC 1 cut(s) 13
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 3 cut(s) 149, 162, 423
AoxI GGCC 3 cut(s) 322, 516, 615
ApoI RAATTY 2 cut(s) 143, 201
AseI ATTAAT 1 cut(s) 108
AspA2I CCTAGG 1 cut(s) 292
AspLEI GCGC 1 cut(s) 353
AspS9I GGNCC 2 cut(s) 80, 256
AsuC2I CCSGG 2 cut(s) 78, 413
AsuHPI GGTGA 2 cut(s) 335, 547
AvaII GGWCC 2 cut(s) 80, 256
AvrII CCTAGG 1 cut(s) 292
BamHI GGATCC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 13
BccI CCATC 2 cut(s) 44, 599
BciT130I CCWGG 3 cut(s) 89, 374, 546
BcnI CCSGG 2 cut(s) 78, 413
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 4 cut(s) 66, 293, 525, 608
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 1 cut(s) 322
BlnI CCTAGG 1 cut(s) 292
BlsI GCNGC 1 cut(s) 323
Bme1390I CCNGG 5 cut(s) 78, 89, 374, 413, 546
Bme18I GGWCC 2 cut(s) 80, 256
BmgT120I GGNCC 2 cut(s) 80, 256
BmiI GGNNCC 2 cut(s) 82, 156
BmrFI CCNGG 5 cut(s) 78, 89, 374, 413, 546
BmrI ACTGGG 1 cut(s) 461
BmuI ACTGGG 1 cut(s) 461
BoxI GACNNNNGTC 1 cut(s) 78
BpmI CTGGAG 1 cut(s) 71
BpuMI CCSGG 2 cut(s) 78, 413
BsaHI GRCGYC 1 cut(s) 579
BsaJI CCNNGG 6 cut(s) 76, 292, 354, 432, 544, 612
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 192, 320
Bse1I ACTGG 2 cut(s) 467, 507
BseBI CCWGG 3 cut(s) 89, 374, 546
BseDI CCNNGG 6 cut(s) 76, 292, 354, 432, 544, 612
BseLI CCNNNNNNNGG 3 cut(s) 123, 192, 320
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 2 cut(s) 467, 507
Bsh1236I CGCG 1 cut(s) 351
BshFI GGCC 3 cut(s) 324, 518, 617
BsiHKAI GWGCWC 1 cut(s) 13
BsiSI CCGG 2 cut(s) 78, 413
BslFI GGGAC 2 cut(s) 66, 513
BslI CCNNNNNNNGG 3 cut(s) 123, 192, 320
BsmAI GTCTC 1 cut(s) 67
BsmFI GGGAC 2 cut(s) 66, 513
BsmI GAATGC 1 cut(s) 44
BsnI GGCC 3 cut(s) 324, 518, 617
Bsp1286I GDGCHC 1 cut(s) 13
Bsp143I GATC 2 cut(s) 154, 415
BspACI CCGC 1 cut(s) 321
BspANI GGCC 3 cut(s) 324, 518, 617
BspCNI CTCAG 1 cut(s) 25
BspFNI CGCG 1 cut(s) 351
BspLI GGNNCC 2 cut(s) 82, 156
BspPI GGATC 3 cut(s) 149, 162, 423
BsrI ACTGG 2 cut(s) 467, 507
BssECI CCNNGG 6 cut(s) 76, 292, 354, 432, 544, 612
BssMI GATC 2 cut(s) 154, 415
BssNI GRCGYC 1 cut(s) 579
BssT1I CCWWGG 3 cut(s) 292, 432, 612
Bst2UI CCWGG 3 cut(s) 89, 374, 546
Bst4CI ACNGT 1 cut(s) 341
BstACI GRCGYC 1 cut(s) 579
BstDEI CTNAG 1 cut(s) 12
BstFNI CGCG 1 cut(s) 351
BstHHI GCGC 1 cut(s) 353
BstKTI GATC 2 cut(s) 157, 418
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 2 cut(s) 154, 415
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstNI CCWGG 3 cut(s) 89, 374, 546
BstPAI GACNNNNGTC 1 cut(s) 78
BstSCI CCNGG 5 cut(s) 76, 87, 372, 411, 544
BstUI CGCG 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 154
BstXI CCANNNNNNTGG 1 cut(s) 514
BstYI RGATCY 1 cut(s) 154
BsuRI GGCC 3 cut(s) 324, 518, 617
BtsIMutI CAGTG 1 cut(s) 514
CfoI GCGC 1 cut(s) 353
Cfr13I GGNCC 2 cut(s) 80, 256
CseI GACGC 1 cut(s) 587
Csp6I GTAC 2 cut(s) 381, 667
CviJI RGCY 8 cut(s) 69, 163, 196, 324, 518, 537, 611, 617
CviKI_1 RGCY 8 cut(s) 69, 163, 196, 324, 518, 537, 611, 617
CviQI GTAC 2 cut(s) 381, 667
DdeI CTNAG 1 cut(s) 12
DpnI GATC 2 cut(s) 156, 417
DpnII GATC 2 cut(s) 154, 415
Eco130I CCWWGG 3 cut(s) 292, 432, 612
Eco147I AGGCCT 1 cut(s) 617
Eco47I GGWCC 2 cut(s) 80, 256
EcoRII CCWGG 3 cut(s) 87, 372, 544
EcoT14I CCWWGG 3 cut(s) 292, 432, 612
EcoT22I ATGCAT 2 cut(s) 29, 571
ErhI CCWWGG 3 cut(s) 292, 432, 612
FaiI YATR 6 cut(s) 25, 302, 309, 504, 560, 646
FaqI GGGAC 2 cut(s) 66, 513
Fnu4HI GCNGC 1 cut(s) 322
Fsp4HI GCNGC 1 cut(s) 322
FspBI CTAG 4 cut(s) 66, 293, 525, 608
GlaI GCGC 1 cut(s) 352
GluI GCNGC 1 cut(s) 322
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 3 cut(s) 324, 518, 617
HapII CCGG 2 cut(s) 78, 413
HgaI GACGC 1 cut(s) 587
HhaI GCGC 1 cut(s) 353
Hin1I GRCGYC 1 cut(s) 579
Hin6I GCGC 1 cut(s) 351
HinP1I GCGC 1 cut(s) 351
HinfI GANTC 1 cut(s) 549
HpaII CCGG 2 cut(s) 78, 413
HphI GGTGA 2 cut(s) 335, 547
Hpy166II GTNNAC 3 cut(s) 136, 256, 383
Hpy188I TCNGA 2 cut(s) 73, 358
Hpy188III TCNNGA 1 cut(s) 599
Hpy8I GTNNAC 3 cut(s) 136, 256, 383
HpyAV CCTTC 3 cut(s) 371, 597, 628
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4IV ACGT 2 cut(s) 390, 404
HpyCH4V TGCA 5 cut(s) 27, 104, 150, 311, 569
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 2 cut(s) 390, 404
Hsp92I GRCGYC 1 cut(s) 579
HspAI GCGC 1 cut(s) 351
Kzo9I GATC 2 cut(s) 154, 415
MaeI CTAG 4 cut(s) 66, 293, 525, 608
MaeII ACGT 2 cut(s) 390, 404
MaeIII GTNAC 4 cut(s) 238, 341, 400, 640
MalI GATC 2 cut(s) 156, 417
MboI GATC 2 cut(s) 154, 415
MboII GAAGA 1 cut(s) 641
MflI RGATCY 1 cut(s) 154
MhlI GDGCHC 1 cut(s) 13
MluCI AATT 4 cut(s) 105, 119, 143, 201
MlyI GAGTC 1 cut(s) 558
MmeI TCCRAC 3 cut(s) 432, 511, 646
MnlI CCTC 6 cut(s) 29, 64, 95, 342, 357, 364
Mph1103I ATGCAT 2 cut(s) 29, 571
MseI TTAA 1 cut(s) 108
MslI CAYNNNNRTG 1 cut(s) 507
MspI CCGG 2 cut(s) 78, 413
MspR9I CCNGG 5 cut(s) 78, 89, 374, 413, 546
Mva1269I GAATGC 1 cut(s) 44
MvaI CCWGG 3 cut(s) 89, 374, 546
MvnI CGCG 1 cut(s) 351
MwoI GCNNNNNNNGC 1 cut(s) 41
NciI CCSGG 2 cut(s) 78, 413
NdeII GATC 2 cut(s) 154, 415
NlaIV GGNNCC 2 cut(s) 82, 156
NmuCI GTSAC 1 cut(s) 341
NsiI ATGCAT 2 cut(s) 29, 571
PceI AGGCCT 1 cut(s) 617
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PctI GAATGC 1 cut(s) 44
PfoI TCCNGGA 2 cut(s) 87, 372
PkrI GCNGC 1 cut(s) 323
PleI GAGTC 1 cut(s) 557
PpsI GAGTC 1 cut(s) 557
PshAI GACNNNNGTC 1 cut(s) 78
PshBI ATTAAT 1 cut(s) 108
Psp6I CCWGG 3 cut(s) 87, 372, 544
PspGI CCWGG 3 cut(s) 87, 372, 544
PspN4I GGNNCC 2 cut(s) 82, 156
PspPI GGNCC 2 cut(s) 80, 256
PsuI RGATCY 1 cut(s) 154
RsaI GTAC 2 cut(s) 382, 668
RsaNI GTAC 2 cut(s) 381, 667
RseI CAYNNNNRTG 1 cut(s) 507
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 1 cut(s) 322
Sau3AI GATC 2 cut(s) 154, 415
Sau96I GGNCC 2 cut(s) 80, 256
SchI GAGTC 1 cut(s) 558
ScrFI CCNGG 5 cut(s) 78, 89, 374, 413, 546
SduI GDGCHC 1 cut(s) 13
SinI GGWCC 2 cut(s) 80, 256
SmiMI CAYNNNNRTG 1 cut(s) 507
Sse9I AATT 4 cut(s) 105, 119, 143, 201
SseBI AGGCCT 1 cut(s) 617
SsiI CCGC 1 cut(s) 321
SspMI CTAG 4 cut(s) 66, 293, 525, 608
StuI AGGCCT 1 cut(s) 617
StyD4I CCNGG 5 cut(s) 76, 87, 372, 411, 544
StyI CCWWGG 3 cut(s) 292, 432, 612
TaaI ACNGT 1 cut(s) 341
TaiI ACGT 2 cut(s) 393, 407
TaqI TCGA 1 cut(s) 627
TaqII GACCGA 1 cut(s) 272
TasI AATT 4 cut(s) 105, 119, 143, 201
TauI GCSGC 1 cut(s) 324
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TscAI CASTG 1 cut(s) 514
TseFI GTSAC 1 cut(s) 341
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 213
TspRI CASTG 1 cut(s) 514
VpaK11BI GGWCC 2 cut(s) 80, 256
VspI ATTAAT 1 cut(s) 108
XapI RAATTY 2 cut(s) 143, 201
XmaJI CCTAGG 1 cut(s) 292
XspI CTAG 4 cut(s) 66, 293, 525, 608
Zsp2I ATGCAT 2 cut(s) 29, 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.