MD14G1243000.v1.1

Germin-like protein subfamily 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000366
Physical Location & Seq
Reverse (-)
32129000 .. 32129955
956 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1243000.v1.1.491

Sequence Viewer

Length: 693 bp
ATGAAAGGAGTTCATTTCCCCGTAACTGCTGCTGCCCTGTCAGTATTAGCATTCGCCACCCTCCTTGCCTCTGCCTCTGATCCCGGTCCTCTTCAGGACTTTTGCGTAGCAATTAATAACACCGATTATGCTGTGTTTGTGAACGGGAAGTTCTGCAAGGACCCAAACCTTGCATCAGCAAACGATTTCTTCACTGACAAGCTCCGGTACCCCGGAAACACATCGAATCCGGTCGGTTCAATTGTTACGGCGGCGAACGTGGATGTTATACCCGGACTCAACACTCTCGGCATATCCTTTGCTCGCGTAGACTTTGCTCCAAATGGCCTCAATCCGCCTCACACTCACCCTCGTGCCACGGAGATCTTCATAGTCTTGGAAGGCTCACTCTACGTCGGATTTGTCACATCCAATATTGAAGGCGACGGCAATCGGCTGTTCACCAAAGTGTTGTACAAGGGAGATGTGTTTGTATTCCCATTCGGTCTCATTCACTTCCAACTCAATGTCGGAAAAACCAATGCTGTGGCCATCGCCGCTTTCAGCAGCCAGAACCCGGGAGTGATCACCGTTGCGAATGCAGTCTTCGGATCCAAACCTCCCATCAACCCTGATGTTTTGACCAAGGCATTCCAGGTGGACAATAATGTGGTTGATTATCTTCAGAAACAGTTTTGGTACAATAACAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

231

Amino Acids

24.65

Weight (kDa)

6.03

Isoelectric Point (pI)

13.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 68 - 218 2.5e-55 Cupin
Cupin_2 PF07883 102 - 179 4.6e-08 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05950 AT5G38930 AT5G38940 AT5G38940 AT5G39130 AT5G39160 AT5G39160 AT5G39160 AT5G39190 AT5G39190
fragaria_vesca FvH4_2g10250 FvH4_3g20400 FvH4_5g18050 FvH4_5g18070 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18470
malus_domestica MD06G1238100.v1.1 MD10G1022500.v1.1 MD10G1022600.v1.1 MD10G1022900.v1.1 MD10G1023100.v1.1 MD10G1023500.v1.1 MD14G1243000.v1.1 MD14G1243100.v1.1
prunus_persica Prupe.5G240700_v2.0.a1 Prupe.8G025500_v2.0.a1 Prupe.8G025600_v2.0.a1 Prupe.8G025700_v2.0.a1 Prupe.8G025800_v2.0.a1 Prupe.8G025900_v2.0.a1 Prupe.8G035600_v2.0.a1 Prupe.8G035700_v2.0.a1 Prupe.8G035800_v2.0.a1 Prupe.8G035900_v2.0.a1 Prupe.8G048300_v2.0.a1
pyrus_communis pycom06g21150 pycom06g21360 pycom06g21370 pycom14g09160 pycom14g09170 pycom14g20410 pycom14g20430 pycom14g20440
rosa_chinensis RchiOBHm_Chr1g0318151 RchiOBHm_Chr1g0354011 RchiOBHm_Chr1g0354031 RchiOBHm_Chr5g0034291 RchiOBHm_Chr7g0177551
rosa_laevigata RLG00000017504 RLG00000028234 RLG00000028236 RLG00000033537
rosa_multiflora Rmu_sc0004015.1_g000016 Rmu_sc0011424.1_g000001 Rmu_sc0011424.1_g000017 Rmu_sc0022466.1_g000003 Rmu_sc0033434.1_g000011 Rmu_ssc0000330.1_g000005 Rmu_ssc0000330.1_g000011
rosa_roxburghii Rroxscaffold_1G00045980 Rroxscaffold_2G00137150 Rroxscaffold_3G00275760 Rroxscaffold_4G00301290 Rroxscaffold_4G00301300 Rroxscaffold_4G00301320 Rroxscaffold_4G00301340 Rroxscaffold_4G00329570 Rroxscaffold_4G00329610 Rroxscaffold_4G00329760 Rroxscaffold_4G00329840 Rroxscaffold_4G00329920
rosa_rugosa Rorug01G0240100 Rorug01G0240200 Rorug01G0240500 Rorug06G0407200 Rorug06G0407300
rosa_samantha Rh1AG031100 Rh1AG251500 Rh1BG223100 Rh1CG029900 Rh1CG235700 Rh1CG235900 Rh1DG250000 Rh2BG197800 Rh5AG236600 Rh5BG237100 Rh5CG266900 Rh5DG244600 Rh7AG006500 Rh7BG006500 Rh7CG006900 Rh7CG007000 Rh7DG006500 Rh7DG006600
rosa_wichuraiana Rw1G002460 Rw1G002500 Rw1G021930 Rw2G014580 Rw7G000540 Rw7G000560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 207
AccB1I GGYRCC 1 cut(s) 207
AccI GTMKAC 1 cut(s) 309
AccII CGCG 1 cut(s) 306
AciI CCGC 3 cut(s) 251, 335, 537
AclWI GGATC 3 cut(s) 74, 585, 598
AcoI YGGCCR 1 cut(s) 528
AcuI CTGAAG 2 cut(s) 77, 647
AfaI GTAC 3 cut(s) 209, 455, 680
AfiI CCNNNNNNNGG 1 cut(s) 556
AgsI TTSAA 2 cut(s) 240, 419
AjnI CCWGG 1 cut(s) 633
AleI CACNNNNGTG 2 cut(s) 351, 446
AluBI AGCT 1 cut(s) 202
AluI AGCT 1 cut(s) 202
Alw26I GTCTC 1 cut(s) 491
AlwI GGATC 3 cut(s) 74, 585, 598
Ama87I CYCGRG 1 cut(s) 556
AoxI GGCC 2 cut(s) 325, 528
ApeKI GCWGC 3 cut(s) 29, 32, 546
AseI ATTAAT 1 cut(s) 114
Asp718I GGTACC 1 cut(s) 207
AspS9I GGNCC 2 cut(s) 86, 160
AsuC2I CCSGG 5 cut(s) 84, 213, 273, 557, 558
AsuHPI GGTGA 3 cut(s) 338, 433, 559
AvaI CYCGRG 1 cut(s) 556
AvaII GGWCC 2 cut(s) 86, 160
BalI TGGCCA 1 cut(s) 530
BamHI GGATCC 1 cut(s) 590
BanI GGYRCC 1 cut(s) 207
BauI CACGAG 1 cut(s) 351
BbsI GAAGAC 1 cut(s) 577
BbvI GCAGC 3 cut(s) 16, 19, 558
BccI CCATC 2 cut(s) 539, 611
BceAI ACGGC 2 cut(s) 264, 442
BciT130I CCWGG 1 cut(s) 635
BclI TGATCA 1 cut(s) 564
BcnI CCSGG 5 cut(s) 84, 213, 273, 557, 558
BcoDI GTCTC 1 cut(s) 491
BglII AGATCT 1 cut(s) 363
BisI GCNGC 5 cut(s) 30, 33, 252, 537, 547
BlsI GCNGC 5 cut(s) 31, 34, 253, 538, 548
Bme1390I CCNGG 6 cut(s) 84, 213, 273, 557, 558, 635
Bme18I GGWCC 2 cut(s) 86, 160
BmeT110I CYCGRG 1 cut(s) 556
BmgT120I GGNCC 2 cut(s) 86, 160
BmiI GGNNCC 3 cut(s) 162, 209, 592
BmrFI CCNGG 6 cut(s) 84, 213, 273, 557, 558, 635
BmsI GCATC 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 577
BpuMI CCSGG 5 cut(s) 84, 213, 273, 557, 558
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 4 cut(s) 211, 357, 556, 624
BsaWI WCCGGW 2 cut(s) 204, 229
Bsc4I CCNNNNNNNGG 1 cut(s) 556
BseBI CCWGG 1 cut(s) 635
BseDI CCNNGG 4 cut(s) 211, 357, 556, 624
BseGI GGATG 2 cut(s) 268, 407
BseLI CCNNNNNNNGG 1 cut(s) 556
BseXI GCAGC 3 cut(s) 16, 19, 558
Bsh1236I CGCG 1 cut(s) 306
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 2 cut(s) 327, 530
BshNI GGYRCC 1 cut(s) 207
BsiEI CGRYCG 1 cut(s) 234
BsiHKCI CYCGRG 1 cut(s) 556
BsiSI CCGG 6 cut(s) 84, 205, 213, 230, 273, 557
BslI CCNNNNNNNGG 1 cut(s) 556
BsmAI GTCTC 1 cut(s) 491
BsmI GAATGC 3 cut(s) 50, 583, 629
BsnI GGCC 2 cut(s) 327, 530
Bso31I GGTCTC 1 cut(s) 491
BsoBI CYCGRG 1 cut(s) 556
Bsp1407I TGTACA 1 cut(s) 453
Bsp143I GATC 4 cut(s) 79, 363, 564, 590
BspACI CCGC 3 cut(s) 251, 335, 537
BspANI GGCC 2 cut(s) 327, 530
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 3 cut(s) 162, 209, 592
BspPI GGATC 3 cut(s) 74, 585, 598
BspT107I GGYRCC 1 cut(s) 207
BspTNI GGTCTC 1 cut(s) 491
BsrGI TGTACA 1 cut(s) 453
BssECI CCNNGG 4 cut(s) 211, 357, 556, 624
BssMI GATC 4 cut(s) 79, 363, 564, 590
BssSI CACGAG 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 624
Bst2BI CACGAG 1 cut(s) 351
Bst2UI CCWGG 1 cut(s) 635
Bst4CI ACNGT 3 cut(s) 571, 672, 689
Bst6I CTCTTC 1 cut(s) 96
BstAUI TGTACA 1 cut(s) 453
BstC8I GCNNGC 1 cut(s) 304
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 2 cut(s) 268, 407
BstFNI CGCG 1 cut(s) 306
BstKTI GATC 4 cut(s) 82, 366, 567, 593
BstMAI GTCTC 1 cut(s) 491
BstMBI GATC 4 cut(s) 79, 363, 564, 590
BstMCI CGRYCG 1 cut(s) 234
BstMWI GCNNNNNNNGC 1 cut(s) 536
BstNI CCWGG 1 cut(s) 635
BstSCI CCNGG 6 cut(s) 82, 211, 271, 555, 556, 633
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 3 cut(s) 16, 19, 558
BstV2I GAAGAC 1 cut(s) 577
BstX2I RGATCY 2 cut(s) 363, 590
BstXI CCANNNNNNTGG 1 cut(s) 526
BstYI RGATCY 2 cut(s) 363, 590
BsuRI GGCC 2 cut(s) 327, 530
BtgI CCRYGG 1 cut(s) 357
BtgZI GCGATG 1 cut(s) 517
BtsCI GGATG 2 cut(s) 268, 407
BtsIMutI CAGTG 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 304
Cfr13I GGNCC 2 cut(s) 86, 160
Cfr9I CCCGGG 1 cut(s) 556
Csp6I GTAC 3 cut(s) 208, 454, 679
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviJI RGCY 6 cut(s) 202, 327, 384, 436, 530, 549
CviKI_1 RGCY 6 cut(s) 202, 327, 384, 436, 530, 549
CviQI GTAC 3 cut(s) 208, 454, 679
DpnI GATC 4 cut(s) 81, 365, 566, 592
DpnII GATC 4 cut(s) 79, 363, 564, 590
EaeI YGGCCR 1 cut(s) 528
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
EciI GGCGGA 1 cut(s) 324
Eco130I CCWWGG 1 cut(s) 624
Eco31I GGTCTC 1 cut(s) 491
Eco47I GGWCC 2 cut(s) 86, 160
Eco57I CTGAAG 2 cut(s) 77, 647
Eco88I CYCGRG 1 cut(s) 556
EcoO109I RGGNCCY 1 cut(s) 160
EcoRII CCWGG 1 cut(s) 633
EcoT14I CCWWGG 1 cut(s) 624
ErhI CCWWGG 1 cut(s) 624
FaiI YATR 4 cut(s) 129, 269, 293, 371
FbaI TGATCA 1 cut(s) 564
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 5 cut(s) 30, 33, 252, 537, 547
FokI GGATG 2 cut(s) 275, 394
Fsp4HI GCNGC 5 cut(s) 30, 33, 252, 537, 547
GluI GCNGC 5 cut(s) 30, 33, 252, 537, 547
HaeIII GGCC 2 cut(s) 327, 530
HapII CCGG 6 cut(s) 84, 205, 213, 230, 273, 557
HinfI GANTC 2 cut(s) 226, 276
HpaII CCGG 6 cut(s) 84, 205, 213, 230, 273, 557
HphI GGTGA 3 cut(s) 338, 433, 559
Hpy166II GTNNAC 4 cut(s) 142, 310, 441, 640
Hpy188I TCNGA 5 cut(s) 79, 398, 512, 590, 666
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 4 cut(s) 142, 310, 441, 640
Hpy99I CGWCG 2 cut(s) 398, 428
HpyAV CCTTC 2 cut(s) 374, 413
HpyCH4III ACNGT 3 cut(s) 571, 672, 689
HpyCH4IV ACGT 2 cut(s) 258, 393
HpyCH4V TGCA 3 cut(s) 156, 173, 581
HpyF10VI GCNNNNNNNGC 1 cut(s) 536
HpySE526I ACGT 2 cut(s) 258, 393
KpnI GGTACC 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 564
Kzo9I GATC 4 cut(s) 79, 363, 564, 590
LmnI GCTCC 2 cut(s) 207, 322
Lsp1109I GCAGC 3 cut(s) 16, 19, 558
LweI GCATC 1 cut(s) 182
MaeII ACGT 2 cut(s) 258, 393
MaeIII GTNAC 3 cut(s) 22, 244, 403
MalI GATC 4 cut(s) 81, 365, 566, 592
MboI GATC 4 cut(s) 79, 363, 564, 590
MboII GAAGA 5 cut(s) 83, 181, 358, 577, 653
MfeI CAATTG 1 cut(s) 240
MflI RGATCY 2 cut(s) 363, 590
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 2 cut(s) 111, 240
MluNI TGGCCA 1 cut(s) 530
MlyI GAGTC 1 cut(s) 270
MmeI TCCRAC 3 cut(s) 376, 490, 523
MnlI CCTC 8 cut(s) 71, 79, 85, 99, 338, 348, 360, 609
Mox20I TGGCCA 1 cut(s) 530
MscI TGGCCA 1 cut(s) 530
MseI TTAA 2 cut(s) 114, 691
MslI CAYNNNNRTG 2 cut(s) 351, 446
Msp20I TGGCCA 1 cut(s) 530
MspI CCGG 6 cut(s) 84, 205, 213, 230, 273, 557
MspR9I CCNGG 6 cut(s) 84, 213, 273, 557, 558, 635
MunI CAATTG 1 cut(s) 240
Mva1269I GAATGC 3 cut(s) 50, 583, 629
MvaI CCWGG 1 cut(s) 635
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 1 cut(s) 536
NciI CCSGG 5 cut(s) 84, 213, 273, 557, 558
NdeII GATC 4 cut(s) 79, 363, 564, 590
NlaIV GGNNCC 3 cut(s) 162, 209, 592
NmeAIII GCCGAG 1 cut(s) 267
NmuCI GTSAC 1 cut(s) 403
OliI CACNNNNGTG 2 cut(s) 351, 446
PctI GAATGC 3 cut(s) 50, 583, 629
PfeI GAWTC 1 cut(s) 226
PkrI GCNGC 5 cut(s) 31, 34, 253, 538, 548
PleI GAGTC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 270
PpuMI RGGWCCY 1 cut(s) 160
PshBI ATTAAT 1 cut(s) 114
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 633
PspGI CCWGG 1 cut(s) 633
PspN4I GGNNCC 3 cut(s) 162, 209, 592
PspPI GGNCC 2 cut(s) 86, 160
PspPPI RGGWCCY 1 cut(s) 160
PsuI RGATCY 2 cut(s) 363, 590
RsaI GTAC 3 cut(s) 209, 455, 680
RsaNI GTAC 3 cut(s) 208, 454, 679
RseI CAYNNNNRTG 2 cut(s) 351, 446
SaqAI TTAA 2 cut(s) 114, 691
SatI GCNGC 5 cut(s) 30, 33, 252, 537, 547
Sau3AI GATC 4 cut(s) 79, 363, 564, 590
Sau96I GGNCC 2 cut(s) 86, 160
SchI GAGTC 1 cut(s) 270
ScrFI CCNGG 6 cut(s) 84, 213, 273, 557, 558, 635
SetI ASST 6 cut(s) 171, 204, 261, 396, 601, 639
SfaNI GCATC 1 cut(s) 182
SinI GGWCC 2 cut(s) 86, 160
SmaI CCCGGG 1 cut(s) 558
SmiMI CAYNNNNRTG 2 cut(s) 351, 446
Sse9I AATT 2 cut(s) 111, 240
SsiI CCGC 3 cut(s) 251, 335, 537
SspI AATATT 1 cut(s) 415
StyD4I CCNGG 6 cut(s) 82, 211, 271, 555, 556, 633
StyI CCWWGG 1 cut(s) 624
TaaI ACNGT 3 cut(s) 571, 672, 689
TaiI ACGT 2 cut(s) 261, 396
TaqI TCGA 1 cut(s) 224
TaqII GACCGA 1 cut(s) 473
TasI AATT 2 cut(s) 111, 240
TatI WGTACW 1 cut(s) 453
TauI GCSGC 2 cut(s) 254, 539
TfiI GAWTC 1 cut(s) 226
Tru1I TTAA 2 cut(s) 114, 691
Tru9I TTAA 2 cut(s) 114, 691
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 3 cut(s) 29, 32, 546
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 2 cut(s) 17, 358
TspGWI ACGGA 1 cut(s) 374
TspMI CCCGGG 1 cut(s) 556
TspRI CASTG 1 cut(s) 199
VpaK11BI GGWCC 2 cut(s) 86, 160
VspI ATTAAT 1 cut(s) 114
XmaI CCCGGG 1 cut(s) 556
XmiI GTMKAC 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.