MD10G1022600.v1.1

Germin-like protein subfamily 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000366
Physical Location & Seq
Reverse (-)
2846309 .. 2846866
558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1022600.v1.1.491

Sequence Viewer

Length: 558 bp
ATGGCAGTGTTTGTGAACGGGAAATTCTGCAAGGACCCAAAGCTTGCATCAGCAAATTATTTCTTCTTTTCTGGGCTTCAAATTCCAAGAAGCACACAAAATCCGTTGGGTTCAACGGTGACACCTGTTAATGTGGACCAAATACCAGGATTAAACACTCTCAGCATATCGCTAGTTCGCATAGACTTTGCACCAAATGGCCTGAACCCTCCCCACACTCACCCTCGCGGCACGGAAATCCTTTTTCTCCAGGAAGGTACGCTCTACGTTGGGTTCGTAACGTCGAACCCGGATAATCGTCTATTCACCAAGGTGTTGAACAAGGGAGATGTATTTGTGTTCCCAGTTGGTCTTATTCACTTTCAACTGAATGTGGGACATACCAATGCTGTCGCCATTTCTGGTCTTAGCAGCCAGAACCCAGGAGTCATCACCATAGCAAATGCAGTGTTTGGCGCCAACCCTCCCATCAATCCTGATGTTCTAGCCAAGGCCTTCCAAGTCGAGAACAAACTGGTTACATATCTTCAAAAACAGTTCTGGTACGACAACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

186

Amino Acids

20.18

Weight (kDa)

8.76

Isoelectric Point (pI)

19.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 25 - 171 2.2e-53 Cupin
Cupin_2 PF07883 61 - 133 3.2e-07 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05950 AT5G38930 AT5G38940 AT5G38940 AT5G39130 AT5G39160 AT5G39160 AT5G39160 AT5G39190 AT5G39190
fragaria_vesca FvH4_2g10250 FvH4_3g20400 FvH4_5g18050 FvH4_5g18070 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18470
malus_domestica MD06G1238100.v1.1 MD10G1022500.v1.1 MD10G1022600.v1.1 MD10G1022900.v1.1 MD10G1023100.v1.1 MD10G1023500.v1.1 MD14G1243000.v1.1 MD14G1243100.v1.1
prunus_persica Prupe.5G240700_v2.0.a1 Prupe.8G025500_v2.0.a1 Prupe.8G025600_v2.0.a1 Prupe.8G025700_v2.0.a1 Prupe.8G025800_v2.0.a1 Prupe.8G025900_v2.0.a1 Prupe.8G035600_v2.0.a1 Prupe.8G035700_v2.0.a1 Prupe.8G035800_v2.0.a1 Prupe.8G035900_v2.0.a1 Prupe.8G048300_v2.0.a1
pyrus_communis pycom06g21150 pycom06g21360 pycom06g21370 pycom14g09160 pycom14g09170 pycom14g20410 pycom14g20430 pycom14g20440
rosa_chinensis RchiOBHm_Chr1g0318151 RchiOBHm_Chr1g0354011 RchiOBHm_Chr1g0354031 RchiOBHm_Chr5g0034291 RchiOBHm_Chr7g0177551
rosa_laevigata RLG00000017504 RLG00000028234 RLG00000028236 RLG00000033537
rosa_multiflora Rmu_sc0004015.1_g000016 Rmu_sc0011424.1_g000001 Rmu_sc0011424.1_g000017 Rmu_sc0022466.1_g000003 Rmu_sc0033434.1_g000011 Rmu_ssc0000330.1_g000005 Rmu_ssc0000330.1_g000011
rosa_roxburghii Rroxscaffold_1G00045980 Rroxscaffold_2G00137150 Rroxscaffold_3G00275760 Rroxscaffold_4G00301290 Rroxscaffold_4G00301300 Rroxscaffold_4G00301320 Rroxscaffold_4G00301340 Rroxscaffold_4G00329570 Rroxscaffold_4G00329610 Rroxscaffold_4G00329760 Rroxscaffold_4G00329840 Rroxscaffold_4G00329920
rosa_rugosa Rorug01G0240100 Rorug01G0240200 Rorug01G0240500 Rorug06G0407200 Rorug06G0407300
rosa_samantha Rh1AG031100 Rh1AG251500 Rh1BG223100 Rh1CG029900 Rh1CG235700 Rh1CG235900 Rh1DG250000 Rh2BG197800 Rh5AG236600 Rh5BG237100 Rh5CG266900 Rh5DG244600 Rh7AG006500 Rh7BG006500 Rh7CG006900 Rh7CG007000 Rh7DG006500 Rh7DG006600
rosa_wichuraiana Rw1G002460 Rw1G002500 Rw1G021930 Rw2G014580 Rw7G000540 Rw7G000560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 455
AccII CGCG 1 cut(s) 228
AciI CCGC 1 cut(s) 228
AcsI RAATTY 2 cut(s) 23, 81
AcyI GRCGYC 1 cut(s) 456
AfaI GTAC 2 cut(s) 259, 545
AgsI TTSAA 5 cut(s) 80, 114, 319, 365, 530
AjnI CCWGG 3 cut(s) 145, 249, 421
AleI CACNNNNGTG 1 cut(s) 311
AluBI AGCT 1 cut(s) 43
AluI AGCT 1 cut(s) 43
AoxI GGCC 2 cut(s) 199, 492
ApeKI GCWGC 1 cut(s) 411
ApoI RAATTY 2 cut(s) 23, 81
AspLEI GCGC 1 cut(s) 458
AspS9I GGNCC 2 cut(s) 34, 136
AsuC2I CCSGG 1 cut(s) 290
AsuHPI GGTGA 4 cut(s) 130, 212, 298, 424
AvaII GGWCC 2 cut(s) 34, 136
BanI GGYRCC 1 cut(s) 455
BbvI GCAGC 1 cut(s) 423
BccI CCATC 1 cut(s) 476
BciT130I CCWGG 3 cut(s) 147, 251, 423
BcnI CCSGG 1 cut(s) 290
BfaI CTAG 2 cut(s) 173, 485
BfoI RGCGCY 1 cut(s) 459
BisI GCNGC 2 cut(s) 229, 412
BlsI GCNGC 2 cut(s) 230, 413
Bme1390I CCNGG 4 cut(s) 147, 251, 290, 423
Bme18I GGWCC 2 cut(s) 34, 136
BmgT120I GGNCC 2 cut(s) 34, 136
BmiI GGNNCC 2 cut(s) 36, 457
BmrFI CCNGG 4 cut(s) 147, 251, 290, 423
BmrI ACTGGG 1 cut(s) 338
BmsI GCATC 1 cut(s) 56
BmuI ACTGGG 1 cut(s) 338
BpmI CTGGAG 1 cut(s) 233
BpuMI CCSGG 1 cut(s) 290
BsaHI GRCGYC 1 cut(s) 456
BsaJI CCNNGG 3 cut(s) 309, 421, 489
Bse1I ACTGG 2 cut(s) 344, 519
BseBI CCWGG 3 cut(s) 147, 251, 423
BseDI CCNNGG 3 cut(s) 309, 421, 489
BseMII CTCAG 1 cut(s) 175
BseNI ACTGG 2 cut(s) 344, 519
BseXI GCAGC 1 cut(s) 423
Bsh1236I CGCG 1 cut(s) 228
BshFI GGCC 2 cut(s) 201, 494
BshNI GGYRCC 1 cut(s) 455
BsiSI CCGG 1 cut(s) 290
BslFI GGGAC 1 cut(s) 390
BsmFI GGGAC 1 cut(s) 390
BsnI GGCC 2 cut(s) 201, 494
BspACI CCGC 1 cut(s) 228
BspANI GGCC 2 cut(s) 201, 494
BspCNI CTCAG 1 cut(s) 174
BspFNI CGCG 1 cut(s) 228
BspLI GGNNCC 2 cut(s) 36, 457
BspT107I GGYRCC 1 cut(s) 455
BsrI ACTGG 2 cut(s) 344, 519
BssECI CCNNGG 3 cut(s) 309, 421, 489
BssNI GRCGYC 1 cut(s) 456
BssT1I CCWWGG 2 cut(s) 309, 489
Bst2UI CCWGG 3 cut(s) 147, 251, 423
Bst4CI ACNGT 2 cut(s) 118, 537
BstACI GRCGYC 1 cut(s) 456
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 2 cut(s) 161, 407
BstFNI CGCG 1 cut(s) 228
BstH2I RGCGCY 1 cut(s) 459
BstHHI GCGC 1 cut(s) 458
BstNI CCWGG 3 cut(s) 147, 251, 423
BstSCI CCNGG 4 cut(s) 145, 249, 288, 421
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 423
BsuRI GGCC 2 cut(s) 201, 494
BtsI GCAGTG 2 cut(s) 12, 453
BtsIMutI CAGTG 2 cut(s) 12, 453
Cac8I GCNNGC 1 cut(s) 45
CfoI GCGC 1 cut(s) 458
Cfr13I GGNCC 2 cut(s) 34, 136
Csp6I GTAC 2 cut(s) 258, 544
CviJI RGCY 6 cut(s) 43, 76, 201, 414, 488, 494
CviKI_1 RGCY 6 cut(s) 43, 76, 201, 414, 488, 494
CviQI GTAC 2 cut(s) 258, 544
DdeI CTNAG 2 cut(s) 161, 407
DinI GGCGCC 1 cut(s) 457
Eco130I CCWWGG 2 cut(s) 309, 489
Eco147I AGGCCT 1 cut(s) 494
Eco47I GGWCC 2 cut(s) 34, 136
EcoO109I RGGNCCY 1 cut(s) 34
EcoRII CCWGG 3 cut(s) 145, 249, 421
EcoT14I CCWWGG 2 cut(s) 309, 489
EgeI GGCGCC 1 cut(s) 457
EheI GGCGCC 1 cut(s) 457
ErhI CCWWGG 2 cut(s) 309, 489
FaiI YATR 5 cut(s) 167, 182, 381, 437, 523
FaqI GGGAC 1 cut(s) 390
Fnu4HI GCNGC 2 cut(s) 229, 412
Fsp4HI GCNGC 2 cut(s) 229, 412
FspBI CTAG 2 cut(s) 173, 485
GlaI GCGC 1 cut(s) 457
GluI GCNGC 2 cut(s) 229, 412
GsuI CTGGAG 1 cut(s) 233
HaeII RGCGCY 1 cut(s) 459
HaeIII GGCC 2 cut(s) 201, 494
HapII CCGG 1 cut(s) 290
HhaI GCGC 1 cut(s) 458
Hin1I GRCGYC 1 cut(s) 456
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 1 cut(s) 426
HpaII CCGG 1 cut(s) 290
HphI GGTGA 4 cut(s) 130, 212, 298, 424
Hpy166II GTNNAC 2 cut(s) 16, 136
Hpy188III TCNNGA 2 cut(s) 476, 505
Hpy8I GTNNAC 2 cut(s) 16, 136
Hpy99I CGWCG 1 cut(s) 286
HpyAV CCTTC 2 cut(s) 248, 505
HpyCH4III ACNGT 2 cut(s) 118, 537
HpyCH4IV ACGT 2 cut(s) 267, 281
HpyCH4V TGCA 4 cut(s) 30, 47, 191, 446
HpyF3I CTNAG 2 cut(s) 161, 407
HpySE526I ACGT 2 cut(s) 267, 281
Hsp92I GRCGYC 1 cut(s) 456
HspAI GCGC 1 cut(s) 456
KasI GGCGCC 1 cut(s) 455
Lsp1109I GCAGC 1 cut(s) 423
LweI GCATC 1 cut(s) 56
MaeI CTAG 2 cut(s) 173, 485
MaeII ACGT 2 cut(s) 267, 281
MaeIII GTNAC 3 cut(s) 118, 277, 517
MboII GAAGA 2 cut(s) 55, 518
MluCI AATT 3 cut(s) 23, 55, 81
Mly113I GGCGCC 1 cut(s) 456
MlyI GAGTC 1 cut(s) 435
MnlI CCTC 3 cut(s) 219, 234, 474
MseI TTAA 2 cut(s) 129, 152
MslI CAYNNNNRTG 2 cut(s) 311, 384
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 4 cut(s) 147, 251, 290, 423
MvaI CCWGG 3 cut(s) 147, 251, 423
MvnI CGCG 1 cut(s) 228
NarI GGCGCC 1 cut(s) 456
NciI CCSGG 1 cut(s) 290
NlaIV GGNNCC 2 cut(s) 36, 457
NmuCI GTSAC 1 cut(s) 118
OliI CACNNNNGTG 1 cut(s) 311
PceI AGGCCT 1 cut(s) 494
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PfoI TCCNGGA 1 cut(s) 249
PkrI GCNGC 2 cut(s) 230, 413
PleI GAGTC 1 cut(s) 434
PluTI GGCGCC 1 cut(s) 459
PpsI GAGTC 1 cut(s) 434
PpuMI RGGWCCY 1 cut(s) 34
Psp5II RGGWCCY 1 cut(s) 34
Psp6I CCWGG 3 cut(s) 145, 249, 421
PspGI CCWGG 3 cut(s) 145, 249, 421
PspN4I GGNNCC 2 cut(s) 36, 457
PspPI GGNCC 2 cut(s) 34, 136
PspPPI RGGWCCY 1 cut(s) 34
RsaI GTAC 2 cut(s) 259, 545
RsaNI GTAC 2 cut(s) 258, 544
RseI CAYNNNNRTG 2 cut(s) 311, 384
SaqAI TTAA 2 cut(s) 129, 152
SatI GCNGC 2 cut(s) 229, 412
Sau96I GGNCC 2 cut(s) 34, 136
SchI GAGTC 1 cut(s) 435
ScrFI CCNGG 4 cut(s) 147, 251, 290, 423
SetI ASST 6 cut(s) 45, 127, 259, 270, 284, 315
SfaNI GCATC 1 cut(s) 56
SfoI GGCGCC 1 cut(s) 457
SinI GGWCC 2 cut(s) 34, 136
SmiMI CAYNNNNRTG 2 cut(s) 311, 384
Sse9I AATT 3 cut(s) 23, 55, 81
SseBI AGGCCT 1 cut(s) 494
SsiI CCGC 1 cut(s) 228
SspDI GGCGCC 1 cut(s) 455
SspMI CTAG 2 cut(s) 173, 485
StuI AGGCCT 1 cut(s) 494
StyD4I CCNGG 4 cut(s) 145, 249, 288, 421
StyI CCWWGG 2 cut(s) 309, 489
TaaI ACNGT 2 cut(s) 118, 537
TaiI ACGT 2 cut(s) 270, 284
TaqI TCGA 2 cut(s) 284, 504
TasI AATT 3 cut(s) 23, 55, 81
TauI GCSGC 1 cut(s) 231
Tru1I TTAA 2 cut(s) 129, 152
Tru9I TTAA 2 cut(s) 129, 152
TscAI CASTG 2 cut(s) 12, 453
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 1 cut(s) 411
Tsp45I GTSAC 1 cut(s) 118
TspGWI ACGGA 2 cut(s) 93, 248
TspRI CASTG 2 cut(s) 12, 453
VpaK11BI GGWCC 2 cut(s) 34, 136
XapI RAATTY 2 cut(s) 23, 81
XspI CTAG 2 cut(s) 173, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.