MD14G1243100.v1.1

Germin-like protein subfamily 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000366
Physical Location & Seq
Reverse (-)
32151154 .. 32152119
966 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1243100.v1.1.491

Sequence Viewer

Length: 687 bp
ATGAAGGGAGTTCATTTCCCTGTAACTGCTGCTGCCCTGTCAGTGTTAGCATTCGCCACCCTCCTTGCCTCTGCCTCTGATCCCGATCCTCTTCAGGACTTTTGCGTAGCAATTAATAACACCGATTCTGCTGTGTTTGTGAACGGGAAGTTCTGCAAGGACCCAAAACTTGCATCAGCAAACGATTTCTTCTCTGACAAGCTACGGTACCCCGGAAACACATCGAATCCGGTCGGTTCAATAGTTACGGCGGCGAACGTGGATGTTATACCCGGACTCAACACTCTCGGCATATCCTTTGCTCGCGTAGACTTTGCGCCAAATGGCCTCAACCCTCCTCACACTCACCCTCGTGCCACGGAGATCCTCATAGTCGTTGAAGGCTCACTCTACGTCGGATTTGTCACATCCAATGGCGACGGCAATCGGCTGTTCACCAAAGTGTTGTACAAGGGAGATGTGTTCGTATTCCCAATCGGTCTCATTCACTTCCAACTCAATGTCGGAAAAACCAATGCTTTGGCCATCGCCGGTCTCAGCAGCCAGAACCCGGGAGTGATCACCATTGCGAATGCAGTCTTCGGATCCAACCCTCCCATCAACCCTGATGTTTTGGCCAAGGCATTCCAGGTGGACGATAATGTGGTTGATTATCTTCAGAAACAGTTTTGGTACAATAACAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

229

Amino Acids

24.24

Weight (kDa)

5.7

Isoelectric Point (pI)

8.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 68 - 216 2.7e-55 Cupin
Cupin_2 PF07883 102 - 173 1.5e-07 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05950 AT5G38930 AT5G38940 AT5G38940 AT5G39130 AT5G39160 AT5G39160 AT5G39160 AT5G39190 AT5G39190
fragaria_vesca FvH4_2g10250 FvH4_3g20400 FvH4_5g18050 FvH4_5g18070 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18470
malus_domestica MD06G1238100.v1.1 MD10G1022500.v1.1 MD10G1022600.v1.1 MD10G1022900.v1.1 MD10G1023100.v1.1 MD10G1023500.v1.1 MD14G1243000.v1.1 MD14G1243100.v1.1
prunus_persica Prupe.5G240700_v2.0.a1 Prupe.8G025500_v2.0.a1 Prupe.8G025600_v2.0.a1 Prupe.8G025700_v2.0.a1 Prupe.8G025800_v2.0.a1 Prupe.8G025900_v2.0.a1 Prupe.8G035600_v2.0.a1 Prupe.8G035700_v2.0.a1 Prupe.8G035800_v2.0.a1 Prupe.8G035900_v2.0.a1 Prupe.8G048300_v2.0.a1
pyrus_communis pycom06g21150 pycom06g21360 pycom06g21370 pycom14g09160 pycom14g09170 pycom14g20410 pycom14g20430 pycom14g20440
rosa_chinensis RchiOBHm_Chr1g0318151 RchiOBHm_Chr1g0354011 RchiOBHm_Chr1g0354031 RchiOBHm_Chr5g0034291 RchiOBHm_Chr7g0177551
rosa_laevigata RLG00000017504 RLG00000028234 RLG00000028236 RLG00000033537
rosa_multiflora Rmu_sc0004015.1_g000016 Rmu_sc0011424.1_g000001 Rmu_sc0011424.1_g000017 Rmu_sc0022466.1_g000003 Rmu_sc0033434.1_g000011 Rmu_ssc0000330.1_g000005 Rmu_ssc0000330.1_g000011
rosa_roxburghii Rroxscaffold_1G00045980 Rroxscaffold_2G00137150 Rroxscaffold_3G00275760 Rroxscaffold_4G00301290 Rroxscaffold_4G00301300 Rroxscaffold_4G00301320 Rroxscaffold_4G00301340 Rroxscaffold_4G00329570 Rroxscaffold_4G00329610 Rroxscaffold_4G00329760 Rroxscaffold_4G00329840 Rroxscaffold_4G00329920
rosa_rugosa Rorug01G0240100 Rorug01G0240200 Rorug01G0240500 Rorug06G0407200 Rorug06G0407300
rosa_samantha Rh1AG031100 Rh1AG251500 Rh1BG223100 Rh1CG029900 Rh1CG235700 Rh1CG235900 Rh1DG250000 Rh2BG197800 Rh5AG236600 Rh5BG237100 Rh5CG266900 Rh5DG244600 Rh7AG006500 Rh7BG006500 Rh7CG006900 Rh7CG007000 Rh7DG006500 Rh7DG006600
rosa_wichuraiana Rw1G002460 Rw1G002500 Rw1G021930 Rw2G014580 Rw7G000540 Rw7G000560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 207
AccB1I GGYRCC 1 cut(s) 207
AccI GTMKAC 1 cut(s) 309
AccII CGCG 1 cut(s) 306
AciI CCGC 1 cut(s) 251
AclWI GGATC 5 cut(s) 74, 80, 358, 579, 592
AcoI YGGCCR 2 cut(s) 522, 615
AcuI CTGAAG 2 cut(s) 77, 641
AfaI GTAC 3 cut(s) 209, 449, 674
AfiI CCNNNNNNNGG 1 cut(s) 550
AgsI TTSAA 2 cut(s) 240, 380
AjnI CCWGG 1 cut(s) 627
AleI CACNNNNGTG 2 cut(s) 351, 440
AluBI AGCT 1 cut(s) 202
AluI AGCT 1 cut(s) 202
Alw26I GTCTC 2 cut(s) 485, 539
AlwI GGATC 5 cut(s) 74, 80, 358, 579, 592
Ama87I CYCGRG 1 cut(s) 550
AoxI GGCC 3 cut(s) 325, 522, 615
ApeKI GCWGC 3 cut(s) 29, 32, 540
AseI ATTAAT 1 cut(s) 114
Asp718I GGTACC 1 cut(s) 207
AspLEI GCGC 1 cut(s) 319
AspS9I GGNCC 1 cut(s) 160
AsuC2I CCSGG 4 cut(s) 213, 273, 551, 552
AsuHPI GGTGA 3 cut(s) 338, 427, 553
AvaI CYCGRG 1 cut(s) 550
AvaII GGWCC 1 cut(s) 160
BalI TGGCCA 2 cut(s) 524, 617
BamHI GGATCC 1 cut(s) 584
BanI GGYRCC 1 cut(s) 207
BauI CACGAG 1 cut(s) 351
BbsI GAAGAC 1 cut(s) 571
BbvI GCAGC 3 cut(s) 16, 19, 552
BccI CCATC 2 cut(s) 533, 605
BceAI ACGGC 2 cut(s) 264, 436
BciT130I CCWGG 1 cut(s) 629
BclI TGATCA 1 cut(s) 558
BcnI CCSGG 4 cut(s) 213, 273, 551, 552
BcoDI GTCTC 2 cut(s) 485, 539
BisI GCNGC 4 cut(s) 30, 33, 252, 541
BlsI GCNGC 4 cut(s) 31, 34, 253, 542
Bme1390I CCNGG 5 cut(s) 213, 273, 551, 552, 629
Bme18I GGWCC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 550
BmgT120I GGNCC 1 cut(s) 160
BmiI GGNNCC 3 cut(s) 162, 209, 586
BmrFI CCNGG 5 cut(s) 213, 273, 551, 552, 629
BmsI GCATC 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 571
BpuMI CCSGG 4 cut(s) 213, 273, 551, 552
BsaBI GATNNNNATC 1 cut(s) 84
BsaI GGTCTC 2 cut(s) 485, 539
BsaJI CCNNGG 4 cut(s) 211, 357, 550, 618
BsaWI WCCGGW 1 cut(s) 229
Bsc4I CCNNNNNNNGG 1 cut(s) 550
Bse118I RCCGGY 1 cut(s) 530
Bse3DI GCAATG 1 cut(s) 564
Bse8I GATNNNNATC 1 cut(s) 84
BseBI CCWGG 1 cut(s) 629
BseDI CCNNGG 4 cut(s) 211, 357, 550, 618
BseGI GGATG 2 cut(s) 268, 407
BseJI GATNNNNATC 1 cut(s) 84
BseLI CCNNNNNNNGG 1 cut(s) 550
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 1 cut(s) 550
BseRI GAGGAG 1 cut(s) 327
BseXI GCAGC 3 cut(s) 16, 19, 552
Bsh1236I CGCG 1 cut(s) 306
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 3 cut(s) 327, 524, 617
BshNI GGYRCC 1 cut(s) 207
BsiEI CGRYCG 1 cut(s) 234
BsiHKCI CYCGRG 1 cut(s) 550
BsiSI CCGG 5 cut(s) 213, 230, 273, 531, 551
BslI CCNNNNNNNGG 1 cut(s) 550
BsmAI GTCTC 2 cut(s) 485, 539
BsmI GAATGC 3 cut(s) 50, 577, 623
BsnI GGCC 3 cut(s) 327, 524, 617
Bso31I GGTCTC 2 cut(s) 485, 539
BsoBI CYCGRG 1 cut(s) 550
Bsp1407I TGTACA 1 cut(s) 447
Bsp143I GATC 5 cut(s) 79, 85, 363, 558, 584
BspACI CCGC 1 cut(s) 251
BspANI GGCC 3 cut(s) 327, 524, 617
BspCNI CTCAG 1 cut(s) 549
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 3 cut(s) 162, 209, 586
BspPI GGATC 5 cut(s) 74, 80, 358, 579, 592
BspT107I GGYRCC 1 cut(s) 207
BspTNI GGTCTC 2 cut(s) 485, 539
BsrDI GCAATG 1 cut(s) 564
BsrFI RCCGGY 1 cut(s) 530
BsrGI TGTACA 1 cut(s) 447
BssAI RCCGGY 1 cut(s) 530
BssECI CCNNGG 4 cut(s) 211, 357, 550, 618
BssMI GATC 5 cut(s) 79, 85, 363, 558, 584
BssSI CACGAG 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 618
Bst2BI CACGAG 1 cut(s) 351
Bst2UI CCWGG 1 cut(s) 629
Bst4CI ACNGT 3 cut(s) 207, 666, 683
Bst6I CTCTTC 1 cut(s) 96
BstAUI TGTACA 1 cut(s) 447
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 1 cut(s) 536
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 2 cut(s) 268, 407
BstFNI CGCG 1 cut(s) 306
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 5 cut(s) 82, 88, 366, 561, 587
BstMAI GTCTC 2 cut(s) 485, 539
BstMBI GATC 5 cut(s) 79, 85, 363, 558, 584
BstMCI CGRYCG 1 cut(s) 234
BstNI CCWGG 1 cut(s) 629
BstSCI CCNGG 5 cut(s) 211, 271, 549, 550, 627
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 3 cut(s) 16, 19, 552
BstV2I GAAGAC 1 cut(s) 571
BstX2I RGATCY 2 cut(s) 363, 584
BstXI CCANNNNNNTGG 1 cut(s) 520
BstYI RGATCY 2 cut(s) 363, 584
BsuRI GGCC 3 cut(s) 327, 524, 617
BtgI CCRYGG 1 cut(s) 357
BtgZI GCGATG 1 cut(s) 511
BtsCI GGATG 2 cut(s) 268, 407
BtsIMutI CAGTG 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 304
CfoI GCGC 1 cut(s) 319
Cfr10I RCCGGY 1 cut(s) 530
Cfr13I GGNCC 1 cut(s) 160
Cfr9I CCCGGG 1 cut(s) 550
Csp6I GTAC 3 cut(s) 208, 448, 673
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviJI RGCY 7 cut(s) 202, 327, 384, 430, 524, 543, 617
CviKI_1 RGCY 7 cut(s) 202, 327, 384, 430, 524, 543, 617
CviQI GTAC 3 cut(s) 208, 448, 673
DdeI CTNAG 1 cut(s) 536
DpnI GATC 5 cut(s) 81, 87, 365, 560, 586
DpnII GATC 5 cut(s) 79, 85, 363, 558, 584
EaeI YGGCCR 2 cut(s) 522, 615
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 618
Eco31I GGTCTC 2 cut(s) 485, 539
Eco47I GGWCC 1 cut(s) 160
Eco57I CTGAAG 2 cut(s) 77, 641
Eco88I CYCGRG 1 cut(s) 550
EcoO109I RGGNCCY 1 cut(s) 160
EcoRII CCWGG 1 cut(s) 627
EcoT14I CCWWGG 1 cut(s) 618
ErhI CCWWGG 1 cut(s) 618
FaiI YATR 3 cut(s) 269, 293, 371
FbaI TGATCA 1 cut(s) 558
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 4 cut(s) 30, 33, 252, 541
FokI GGATG 2 cut(s) 275, 394
Fsp4HI GCNGC 4 cut(s) 30, 33, 252, 541
GlaI GCGC 1 cut(s) 318
GluI GCNGC 4 cut(s) 30, 33, 252, 541
HaeIII GGCC 3 cut(s) 327, 524, 617
HapII CCGG 5 cut(s) 213, 230, 273, 531, 551
HhaI GCGC 1 cut(s) 319
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HinfI GANTC 3 cut(s) 125, 226, 276
HpaII CCGG 5 cut(s) 213, 230, 273, 531, 551
HphI GGTGA 3 cut(s) 338, 427, 553
Hpy166II GTNNAC 4 cut(s) 142, 310, 435, 634
Hpy188I TCNGA 6 cut(s) 79, 196, 398, 506, 584, 660
Hpy188III TCNNGA 2 cut(s) 83, 95
Hpy8I GTNNAC 4 cut(s) 142, 310, 435, 634
Hpy99I CGWCG 2 cut(s) 398, 422
HpyAV CCTTC 1 cut(s) 374
HpyCH4III ACNGT 3 cut(s) 207, 666, 683
HpyCH4IV ACGT 2 cut(s) 258, 393
HpyCH4V TGCA 3 cut(s) 156, 173, 575
HpyF3I CTNAG 1 cut(s) 536
HpySE526I ACGT 2 cut(s) 258, 393
HspAI GCGC 1 cut(s) 317
KpnI GGTACC 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 558
Kzo9I GATC 5 cut(s) 79, 85, 363, 558, 584
Lsp1109I GCAGC 3 cut(s) 16, 19, 552
LweI GCATC 1 cut(s) 182
MaeII ACGT 2 cut(s) 258, 393
MaeIII GTNAC 3 cut(s) 22, 244, 403
MalI GATC 5 cut(s) 81, 87, 365, 560, 586
MboI GATC 5 cut(s) 79, 85, 363, 558, 584
MboII GAAGA 4 cut(s) 83, 181, 571, 647
MflI RGATCY 2 cut(s) 363, 584
MlsI TGGCCA 2 cut(s) 524, 617
MluCI AATT 1 cut(s) 111
MluNI TGGCCA 2 cut(s) 524, 617
MlyI GAGTC 1 cut(s) 270
MmeI TCCRAC 4 cut(s) 376, 484, 517, 612
Mox20I TGGCCA 2 cut(s) 524, 617
MscI TGGCCA 2 cut(s) 524, 617
MseI TTAA 2 cut(s) 114, 685
MslI CAYNNNNRTG 2 cut(s) 351, 440
Msp20I TGGCCA 2 cut(s) 524, 617
MspI CCGG 5 cut(s) 213, 230, 273, 531, 551
MspR9I CCNGG 5 cut(s) 213, 273, 551, 552, 629
Mva1269I GAATGC 3 cut(s) 50, 577, 623
MvaI CCWGG 1 cut(s) 629
MvnI CGCG 1 cut(s) 306
NciI CCSGG 4 cut(s) 213, 273, 551, 552
NdeII GATC 5 cut(s) 79, 85, 363, 558, 584
NlaIV GGNNCC 3 cut(s) 162, 209, 586
NmeAIII GCCGAG 1 cut(s) 267
NmuCI GTSAC 1 cut(s) 403
OliI CACNNNNGTG 2 cut(s) 351, 440
PctI GAATGC 3 cut(s) 50, 577, 623
PfeI GAWTC 2 cut(s) 125, 226
PkrI GCNGC 4 cut(s) 31, 34, 253, 542
PleI GAGTC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 270
PpuMI RGGWCCY 1 cut(s) 160
PshBI ATTAAT 1 cut(s) 114
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 627
PspGI CCWGG 1 cut(s) 627
PspN4I GGNNCC 3 cut(s) 162, 209, 586
PspPI GGNCC 1 cut(s) 160
PspPPI RGGWCCY 1 cut(s) 160
PsuI RGATCY 2 cut(s) 363, 584
RsaI GTAC 3 cut(s) 209, 449, 674
RsaNI GTAC 3 cut(s) 208, 448, 673
RseI CAYNNNNRTG 2 cut(s) 351, 440
SaqAI TTAA 2 cut(s) 114, 685
SatI GCNGC 4 cut(s) 30, 33, 252, 541
Sau3AI GATC 5 cut(s) 79, 85, 363, 558, 584
Sau96I GGNCC 1 cut(s) 160
SchI GAGTC 1 cut(s) 270
ScrFI CCNGG 5 cut(s) 213, 273, 551, 552, 629
SetI ASST 4 cut(s) 204, 261, 396, 633
SfaNI GCATC 1 cut(s) 182
SinI GGWCC 1 cut(s) 160
SmaI CCCGGG 1 cut(s) 552
SmiMI CAYNNNNRTG 2 cut(s) 351, 440
Sse9I AATT 1 cut(s) 111
SsiI CCGC 1 cut(s) 251
StyD4I CCNGG 5 cut(s) 211, 271, 549, 550, 627
StyI CCWWGG 1 cut(s) 618
TaaI ACNGT 3 cut(s) 207, 666, 683
TaiI ACGT 2 cut(s) 261, 396
TaqI TCGA 1 cut(s) 224
TaqII GACCGA 1 cut(s) 467
TasI AATT 1 cut(s) 111
TatI WGTACW 1 cut(s) 447
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 2 cut(s) 125, 226
Tru1I TTAA 2 cut(s) 114, 685
Tru9I TTAA 2 cut(s) 114, 685
TscAI CASTG 1 cut(s) 48
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 3 cut(s) 29, 32, 540
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 374
TspMI CCCGGG 1 cut(s) 550
TspRI CASTG 1 cut(s) 48
VpaK11BI GGWCC 1 cut(s) 160
VspI ATTAAT 1 cut(s) 114
XmaI CCCGGG 1 cut(s) 550
XmiI GTMKAC 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.