Rroxscaffold_4G00301300

Germin-like protein subfamily 1 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000366
Physical Location & Seq
Reverse (-)
21695491 .. 21698837
3347 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301300.1

Sequence Viewer

Length: 795 bp
ATGATGATGAAAGGTGCTCATTTCCCTACAGCTGCTGCTTTTGCCCTATTGGCATTGGCAACCTTCCACCTTGTATCTGCCTCTGATCCTAGTCCTCTCCAAGATTTTTGTGTAGCACTTAACACCACAGAAACTGTTTTTTCTGCCGTGTTTGTGAATGGAAAATTCTGCAGGGACCCAAAGCTTGCAACAGCAGATGATTTCTTCTTTTCCGGGCTCCGGATTCCTGGAAACACATCAAATCCGCTAGGTTCCAAAGTCACCCCAGCTAATGTGGAGCAAATAGCAGGGCTGAACACTCTTGGGATATCCCTGGCTCGCATAGACTTTGCACCAAATGGCGGACTAAACCCCCCTCACACCCACCCTCGAGCCACGGAAGTCCTTATGGTCATGGAAGGCACTCTCTACGTTGGCTTCGTCACATCCGATAACCGCCTCTTCACCAAAGTGTTGTACAAGGGAGATGTGTTTGTCTTCCCAGTTGGTCTCATTCACTTCCAGTTCAACATCGGAAAGGTTAAAGCCATAGCCATTGCCGCTCTTAGCAGCCAGAATCCGGGTGTCATCACCATAGCCAATGCAGTGTTCGGCTCCAACCCTCCAATCAACCTTGATGTTCTCACCAAGGCCTTCCAAGTTGACAACAATGTCGTTCAGTATCTCCAGAAACAGTTCTGCAACTTGGGATGCAACAAATGCACACTTGTTGGAGTGTTTTCGAGTTTCTGTGGAGTCAAGATACCAAAGAGTACGAAGAGCAAGAATGATGAGGCAAAGGGCACAAAGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

264

Amino Acids

28.2

Weight (kDa)

9.03

Isoelectric Point (pI)

17.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 72 - 220 1.3e-52 Cupin
Cupin_2 PF07883 108 - 177 3.3e-07 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05950 AT5G38930 AT5G38940 AT5G38940 AT5G39130 AT5G39160 AT5G39160 AT5G39160 AT5G39190 AT5G39190
fragaria_vesca FvH4_2g10250 FvH4_3g20400 FvH4_5g18050 FvH4_5g18070 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18460 FvH4_5g18470
malus_domestica MD06G1238100.v1.1 MD10G1022500.v1.1 MD10G1022600.v1.1 MD10G1022900.v1.1 MD10G1023100.v1.1 MD10G1023500.v1.1 MD14G1243000.v1.1 MD14G1243100.v1.1
prunus_persica Prupe.5G240700_v2.0.a1 Prupe.8G025500_v2.0.a1 Prupe.8G025600_v2.0.a1 Prupe.8G025700_v2.0.a1 Prupe.8G025800_v2.0.a1 Prupe.8G025900_v2.0.a1 Prupe.8G035600_v2.0.a1 Prupe.8G035700_v2.0.a1 Prupe.8G035800_v2.0.a1 Prupe.8G035900_v2.0.a1 Prupe.8G048300_v2.0.a1
pyrus_communis pycom06g21150 pycom06g21360 pycom06g21370 pycom14g09160 pycom14g09170 pycom14g20410 pycom14g20430 pycom14g20440
rosa_chinensis RchiOBHm_Chr1g0318151 RchiOBHm_Chr1g0354011 RchiOBHm_Chr1g0354031 RchiOBHm_Chr5g0034291 RchiOBHm_Chr7g0177551
rosa_laevigata RLG00000017504 RLG00000028234 RLG00000028236 RLG00000033537
rosa_multiflora Rmu_sc0004015.1_g000016 Rmu_sc0011424.1_g000001 Rmu_sc0011424.1_g000017 Rmu_sc0022466.1_g000003 Rmu_sc0033434.1_g000011 Rmu_ssc0000330.1_g000005 Rmu_ssc0000330.1_g000011
rosa_roxburghii Rroxscaffold_1G00045980 Rroxscaffold_2G00137150 Rroxscaffold_3G00275760 Rroxscaffold_4G00301290 Rroxscaffold_4G00301300 Rroxscaffold_4G00301320 Rroxscaffold_4G00301340 Rroxscaffold_4G00329570 Rroxscaffold_4G00329610 Rroxscaffold_4G00329760 Rroxscaffold_4G00329840 Rroxscaffold_4G00329920
rosa_rugosa Rorug01G0240100 Rorug01G0240200 Rorug01G0240500 Rorug06G0407200 Rorug06G0407300
rosa_samantha Rh1AG031100 Rh1AG251500 Rh1BG223100 Rh1CG029900 Rh1CG235700 Rh1CG235900 Rh1DG250000 Rh2BG197800 Rh5AG236600 Rh5BG237100 Rh5CG266900 Rh5DG244600 Rh7AG006500 Rh7BG006500 Rh7CG006900 Rh7CG007000 Rh7DG006500 Rh7DG006600
rosa_wichuraiana Rw1G002460 Rw1G002500 Rw1G021930 Rw2G014580 Rw7G000540 Rw7G000560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 650
AccBSI CCGCTC 1 cut(s) 542
AccIII TCCGGA 1 cut(s) 219
AciI CCGC 4 cut(s) 245, 342, 436, 540
AclWI GGATC 1 cut(s) 80
AcsI RAATTY 1 cut(s) 164
AfaI GTAC 2 cut(s) 458, 754
AfiI CCNNNNNNNGG 3 cut(s) 219, 341, 559
AgsI TTSAA 1 cut(s) 508
AjnI CCWGG 2 cut(s) 226, 312
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
AleI CACNNNNGTG 1 cut(s) 449
AluBI AGCT 3 cut(s) 32, 184, 269
AluI AGCT 3 cut(s) 32, 184, 269
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 1 cut(s) 494
AlwI GGATC 1 cut(s) 80
AlwNI CAGNNNCTG 2 cut(s) 35, 134
Ama87I CYCGRG 1 cut(s) 369
Aor13HI TCCGGA 1 cut(s) 219
AoxI GGCC 1 cut(s) 630
ApeKI GCWGC 3 cut(s) 32, 35, 549
ApoI RAATTY 1 cut(s) 164
Asp700I GAANNNNTTC 1 cut(s) 674
AspS9I GGNCC 1 cut(s) 175
AsuC2I CCSGG 2 cut(s) 214, 561
AsuHPI GGTGA 4 cut(s) 253, 436, 562, 616
AvaI CYCGRG 1 cut(s) 369
AvaII GGWCC 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 785
BanII GRGCYC 1 cut(s) 219
BbsI GAAGAC 1 cut(s) 469
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 3 cut(s) 19, 22, 561
BceAI ACGGC 1 cut(s) 131
BciT130I CCWGG 2 cut(s) 228, 314
BcnI CCSGG 2 cut(s) 214, 561
BcoDI GTCTC 1 cut(s) 494
BfaI CTAG 2 cut(s) 90, 248
BfmI CTRYAG 2 cut(s) 27, 169
BglI GCCNNNNNGGC 1 cut(s) 50
BisI GCNGC 4 cut(s) 33, 36, 540, 550
BlsI GCNGC 4 cut(s) 34, 37, 541, 551
Bme1390I CCNGG 4 cut(s) 214, 228, 314, 561
Bme18I GGWCC 1 cut(s) 175
BmeT110I CYCGRG 1 cut(s) 369
BmgT120I GGNCC 1 cut(s) 175
BmiI GGNNCC 5 cut(s) 176, 177, 218, 253, 595
BmrFI CCNGG 4 cut(s) 214, 228, 314, 561
BmrI ACTGGG 1 cut(s) 476
BmsI GCATC 1 cut(s) 680
BmuI ACTGGG 1 cut(s) 476
BpiI GAAGAC 1 cut(s) 469
BpmI CTGGAG 1 cut(s) 650
BpuMI CCSGG 2 cut(s) 214, 561
BsaI GGTCTC 1 cut(s) 494
BsaJI CCNNGG 3 cut(s) 312, 375, 627
BsaWI WCCGGW 1 cut(s) 219
Bsc4I CCNNNNNNNGG 3 cut(s) 219, 341, 559
Bse1I ACTGG 2 cut(s) 482, 502
Bse3DI GCAATG 1 cut(s) 534
BseAI TCCGGA 1 cut(s) 219
BseBI CCWGG 2 cut(s) 228, 314
BseDI CCNNGG 3 cut(s) 312, 375, 627
BseGI GGATG 2 cut(s) 425, 695
BseLI CCNNNNNNNGG 3 cut(s) 219, 341, 559
BseMI GCAATG 1 cut(s) 534
BseNI ACTGG 2 cut(s) 482, 502
BseSI GKGCMC 1 cut(s) 785
BseXI GCAGC 3 cut(s) 19, 22, 561
BseYI CCCAGC 1 cut(s) 265
BshFI GGCC 1 cut(s) 632
BsiHKAI GWGCWC 1 cut(s) 19
BsiHKCI CYCGRG 1 cut(s) 369
BsiSI CCGG 3 cut(s) 213, 220, 560
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 3 cut(s) 219, 341, 559
BsmAI GTCTC 1 cut(s) 494
BsmFI GGGAC 1 cut(s) 188
BsnI GGCC 1 cut(s) 632
Bso31I GGTCTC 1 cut(s) 494
BsoBI CYCGRG 1 cut(s) 369
Bsp1286I GDGCHC 3 cut(s) 19, 219, 785
Bsp13I TCCGGA 1 cut(s) 219
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 1 cut(s) 85
BspACI CCGC 4 cut(s) 245, 342, 436, 540
BspANI GGCC 1 cut(s) 632
BspEI TCCGGA 1 cut(s) 219
BspLI GGNNCC 5 cut(s) 176, 177, 218, 253, 595
BspMAI CTGCAG 1 cut(s) 173
BspPI GGATC 1 cut(s) 80
BspQI GCTCTTC 1 cut(s) 752
BspTNI GGTCTC 1 cut(s) 494
BsrBI CCGCTC 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 534
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 2 cut(s) 482, 502
BssECI CCNNGG 3 cut(s) 312, 375, 627
BssMI GATC 1 cut(s) 85
BssT1I CCWWGG 1 cut(s) 627
Bst2UI CCWGG 2 cut(s) 228, 314
Bst4CI ACNGT 2 cut(s) 136, 675
Bst6I CTCTTC 2 cut(s) 446, 752
BstAPI GCANNNNNTGC 1 cut(s) 699
BstAUI TGTACA 1 cut(s) 456
BstC8I GCNNGC 2 cut(s) 186, 319
BstDEI CTNAG 1 cut(s) 545
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 425, 695
BstKTI GATC 1 cut(s) 88
BstMAI GTCTC 1 cut(s) 494
BstMBI GATC 1 cut(s) 85
BstMWI GCNNNNNNNGC 4 cut(s) 41, 50, 539, 699
BstNI CCWGG 2 cut(s) 228, 314
BstSCI CCNGG 4 cut(s) 212, 226, 312, 559
BstSFI CTRYAG 2 cut(s) 27, 169
BstSLI GKGCMC 1 cut(s) 785
BstV1I GCAGC 3 cut(s) 19, 22, 561
BstV2I GAAGAC 1 cut(s) 469
BsuRI GGCC 1 cut(s) 632
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 425, 695
BtsI GCAGTG 1 cut(s) 591
BtsIMutI CAGTG 1 cut(s) 591
Cac8I GCNNGC 2 cut(s) 186, 319
CaiI CAGNNNCTG 2 cut(s) 35, 134
Cfr13I GGNCC 1 cut(s) 175
Csp6I GTAC 2 cut(s) 457, 753
CviAII CATG 1 cut(s) 394
CviQI GTAC 2 cut(s) 457, 753
DdeI CTNAG 1 cut(s) 545
DpnI GATC 1 cut(s) 87
DpnII GATC 1 cut(s) 85
DrdI GACNNNNNNGTC 1 cut(s) 650
DseDI GACNNNNNNGTC 1 cut(s) 650
Eam1104I CTCTTC 2 cut(s) 446, 752
EarI CTCTTC 2 cut(s) 446, 752
EciI GGCGGA 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 627
Eco147I AGGCCT 1 cut(s) 632
Eco24I GRGCYC 1 cut(s) 219
Eco31I GGTCTC 1 cut(s) 494
Eco32I GATATC 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 175
Eco88I CYCGRG 1 cut(s) 369
EcoO109I RGGNCCY 1 cut(s) 175
EcoRII CCWGG 2 cut(s) 226, 312
EcoRV GATATC 1 cut(s) 309
EcoT14I CCWWGG 1 cut(s) 627
EcoT38I GRGCYC 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 627
FaeI CATG 1 cut(s) 397
FaiI YATR 5 cut(s) 323, 389, 395, 530, 575
FaqI GGGAC 1 cut(s) 188
FatI CATG 1 cut(s) 393
Fnu4HI GCNGC 4 cut(s) 33, 36, 540, 550
FokI GGATG 2 cut(s) 412, 702
FriOI GRGCYC 1 cut(s) 219
Fsp4HI GCNGC 4 cut(s) 33, 36, 540, 550
FspBI CTAG 2 cut(s) 90, 248
GluI GCNGC 4 cut(s) 33, 36, 540, 550
GsaI CCCAGC 1 cut(s) 269
GsuI CTGGAG 1 cut(s) 650
HaeIII GGCC 1 cut(s) 632
HapII CCGG 3 cut(s) 213, 220, 560
Hin1II CATG 1 cut(s) 397
HincII GTYRAC 1 cut(s) 643
HindII GTYRAC 1 cut(s) 643
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 3 cut(s) 223, 556, 735
HpaII CCGG 3 cut(s) 213, 220, 560
HphI GGTGA 4 cut(s) 253, 436, 562, 616
Hpy166II GTNNAC 1 cut(s) 643
Hpy188I TCNGA 3 cut(s) 85, 430, 515
Hpy188III TCNNGA 3 cut(s) 220, 667, 739
Hpy8I GTNNAC 1 cut(s) 643
HpyAV CCTTC 3 cut(s) 73, 392, 643
HpyCH4III ACNGT 2 cut(s) 136, 675
HpyCH4IV ACGT 1 cut(s) 411
HpyCH4V TGCA 7 cut(s) 171, 188, 332, 584, 681, 693, 702
HpyF10VI GCNNNNNNNGC 4 cut(s) 41, 50, 539, 699
HpyF3I CTNAG 1 cut(s) 545
HpySE526I ACGT 1 cut(s) 411
Hsp92II CATG 1 cut(s) 397
KflI GGGWCCC 1 cut(s) 175
Kpn2I TCCGGA 1 cut(s) 219
Kzo9I GATC 1 cut(s) 85
LguI GCTCTTC 1 cut(s) 752
LmnI GCTCC 3 cut(s) 222, 277, 599
Lsp1109I GCAGC 3 cut(s) 19, 22, 561
LweI GCATC 1 cut(s) 680
MaeI CTAG 2 cut(s) 90, 248
MaeII ACGT 1 cut(s) 411
MaeIII GTNAC 2 cut(s) 259, 421
MalI GATC 1 cut(s) 87
MbiI CCGCTC 1 cut(s) 542
MboI GATC 1 cut(s) 85
MboII GAAGA 4 cut(s) 196, 433, 469, 769
MhlI GDGCHC 3 cut(s) 19, 219, 785
MluCI AATT 1 cut(s) 164
MlyI GAGTC 1 cut(s) 744
MmeI TCCRAC 2 cut(s) 621, 691
MnlI CCTC 7 cut(s) 91, 105, 366, 378, 449, 612, 766
MroI TCCGGA 1 cut(s) 219
MroXI GAANNNNTTC 1 cut(s) 674
MseI TTAA 2 cut(s) 120, 522
MslI CAYNNNNRTG 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 3 cut(s) 213, 220, 560
MspR9I CCNGG 4 cut(s) 214, 228, 314, 561
MvaI CCWGG 2 cut(s) 228, 314
MwoI GCNNNNNNNGC 4 cut(s) 41, 50, 539, 699
NciI CCSGG 2 cut(s) 214, 561
NdeII GATC 1 cut(s) 85
NlaIII CATG 1 cut(s) 397
NlaIV GGNNCC 5 cut(s) 176, 177, 218, 253, 595
NmuCI GTSAC 2 cut(s) 259, 421
OliI CACNNNNGTG 1 cut(s) 449
PaeR7I CTCGAG 1 cut(s) 369
PceI AGGCCT 1 cut(s) 632
PciSI GCTCTTC 1 cut(s) 752
PcsI WCGNNNNNNNCGW 2 cut(s) 417, 426
PdmI GAANNNNTTC 1 cut(s) 674
PfeI GAWTC 2 cut(s) 223, 556
PfoI TCCNGGA 1 cut(s) 226
PkrI GCNGC 4 cut(s) 34, 37, 541, 551
PleI GAGTC 1 cut(s) 743
PpsI GAGTC 1 cut(s) 743
PpuMI RGGWCCY 1 cut(s) 175
Psp5II RGGWCCY 1 cut(s) 175
Psp6I CCWGG 2 cut(s) 226, 312
PspFI CCCAGC 1 cut(s) 265
PspGI CCWGG 2 cut(s) 226, 312
PspN4I GGNNCC 5 cut(s) 176, 177, 218, 253, 595
PspPI GGNCC 1 cut(s) 175
PspPPI RGGWCCY 1 cut(s) 175
PspXI VCTCGAGB 1 cut(s) 369
PstI CTGCAG 1 cut(s) 173
PstNI CAGNNNCTG 2 cut(s) 35, 134
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 2 cut(s) 458, 754
RsaNI GTAC 2 cut(s) 457, 753
RseI CAYNNNNRTG 1 cut(s) 449
SapI GCTCTTC 1 cut(s) 752
SaqAI TTAA 2 cut(s) 120, 522
SatI GCNGC 4 cut(s) 33, 36, 540, 550
Sau3AI GATC 1 cut(s) 85
Sau96I GGNCC 1 cut(s) 175
SchI GAGTC 1 cut(s) 744
ScrFI CCNGG 4 cut(s) 214, 228, 314, 561
SduI GDGCHC 3 cut(s) 19, 219, 785
SfaNI GCATC 1 cut(s) 680
SfcI CTRYAG 2 cut(s) 27, 169
Sfr274I CTCGAG 1 cut(s) 369
SinI GGWCC 1 cut(s) 175
SlaI CTCGAG 1 cut(s) 369
SmiMI CAYNNNNRTG 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 369
SmoI CTYRAG 1 cut(s) 369
Sse9I AATT 1 cut(s) 164
SseBI AGGCCT 1 cut(s) 632
SsiI CCGC 4 cut(s) 245, 342, 436, 540
SspMI CTAG 2 cut(s) 90, 248
StuI AGGCCT 1 cut(s) 632
StyD4I CCNGG 4 cut(s) 212, 226, 312, 559
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 2 cut(s) 136, 675
TaiI ACGT 1 cut(s) 414
TaqI TCGA 2 cut(s) 370, 722
TasI AATT 1 cut(s) 164
TatI WGTACW 1 cut(s) 456
TauI GCSGC 1 cut(s) 542
TfiI GAWTC 2 cut(s) 223, 556
Tru1I TTAA 2 cut(s) 120, 522
Tru9I TTAA 2 cut(s) 120, 522
TscAI CASTG 1 cut(s) 591
TseFI GTSAC 2 cut(s) 259, 421
TseI GCWGC 3 cut(s) 32, 35, 549
Tsp45I GTSAC 2 cut(s) 259, 421
TspDTI ATGAA 1 cut(s) 23
TspGWI ACGGA 1 cut(s) 392
TspRI CASTG 1 cut(s) 591
VpaK11BI GGWCC 1 cut(s) 175
XapI RAATTY 1 cut(s) 164
XhoI CTCGAG 1 cut(s) 369
XmnI GAANNNNTTC 1 cut(s) 674
XspI CTAG 2 cut(s) 90, 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.