Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0001209

Physical Location & Seq

Genomic Coordinates Reverse (-)

12060320 .. 12062291

Gene Structure 1972 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g20390.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1176 bp

ATGGTTGGGCTGACCCAGGCACGCACCAGGAGTGGCCCTATCGTGCCGGGAGGGACTCCTTTTCCCTCGCCGCACCGCGCCACCCGCCCACGTCAGCATTACCTCACCGCCTTCATCCACCACCAACCACAATGCCCACCCTTATATCTCTCCTCCTCCACCAAACCCACCACCTCTCCAACAATCATGTCCACCACCGTCGTCACCAAACCCCGCCGGGTCCCACCGCCCTGCTGGACCCAGGACGAGGCCCAATCCCTCATCCTCGCCTACCGCGACCGCTGGTTCGCCCTCCGCCGCGCCAATCTCAAAGCCGCCGACTGGGACCACGTCGCCAAGGCCACCGCCGCCGCTTCCTCCTCCTCCTCCTCGCCGCCGAAGACCGCGCTGCAGTGCCGCCATAAGATGGAGAAGCTTCGCAAGCGGTACCGCGCTGAGAAGCAACGCGCTTCCAACTACCCGGGTCGGTTCTTCTCGTCGTGGGCTTTGTTTCATCTCATGGATGAGATGGAGTCCGACCCGGATCGGAAACCGATTGATGAGGGTGAGTTTGGAGGTAAGGGTCGTAATTTGGTGAATGTTGATAGGAGTTTTAGCCCTAATGGTGATTTTGATGGAATTGGGGGGAGTGTGTTTGATCTAGGAAATGAATTGTATCAGAATTCTAGGTTTAACTACAGATTTGGTGAGAATTCTGGCTTTTATTCTGAGGATAATGAGTATGTTAATCGAGGCGGGTTTCCTTCGAGAACTAGGAGTAATGGCAAGAGTGATGTGAATCTTAACAACAGTTTTAACCAACATAGGGACTATGGAATTAGAAGTGATAGAGACTATGTACCCAGTGGGAGTTTGAAGCGCAATGCCCATTGTAAGGAGTTTGATGGGTATGCTTCTTTATCGGGATCCAAGTTTGGGAAGAAGAGAAGTGTTGTTGGGATGGAGAGAGATGTGGATCCTGTTGCAGACTTGGCTTCCTCGATTATGATGTTGGGAGAGGGGTTTGAGAGGATGGAGAGAATGAAGATGGAAATGACCTGGGAGATTGAGAAGAGGCGGATGGAGACGGAGCTTAAGCGTACTGAGGCCATGAACGAATCACTCCAGCAGTTTGTGGATGCATTGTTGGAAAGGAAGAAGAAAAAGAAGGCGATGGTAGAGTCTTCCCATGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-bind_4

Protein Analysis

392

Amino Acids

44.43

Weight (kDa)

9.61

Isoelectric Point (pI)

53.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-bind_4	PF13837	77 - 170	1.2e-15	Myb/SANT-like DNA-binding domain
MADF_DNA_bdg	PF10545	99 - 167	5.9e-06	Alcohol dehydrogenase transcription factor Myb/SANT-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001209)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g20390 FvH4_5g20450
malus_domestica	MD04G1055300.v1.1 MD06G1046900.v1.1
prunus_persica	Prupe.5G055100_v2.0.a1
pyrus_communis	pycom04g04820 pycom06g03940
rosa_chinensis	RchiOBHm_Chr3g0463421 RchiOBHm_Chr3g0465461 RchiOBHm_Chr6g0286391 RchiOBHm_Chr7g0205651 RchiOBHm_Chr7g0205761
rosa_laevigata	RLG00000003356 RLG00000003367 RLG00000014149 RLG00000036554
rosa_multiflora	Rmu_co8189974.1_g000001 Rmu_co8301667.1_g000001 Rmu_sc0000076.1_g000052 Rmu_sc0000215.1_g000019 Rmu_sc0000233.1_g000008 Rmu_sc0000524.1_g000010 Rmu_sc0001711.1_g000030 Rmu_sc0001836.1_g000048 Rmu_sc0006124.1_g000002 Rmu_sc0008518.1_g000014 Rmu_ssc0000339.1_g000018
rosa_roxburghii	Rroxscaffold_1G00022950 Rroxscaffold_1G00023180 Rroxscaffold_3G00252000 Rroxscaffold_3G00252100 Rroxscaffold_5G00376460 Rroxscaffold_7G00200010 Rroxscaffold_7G00208540
rosa_rugosa	Rorug01G0167500 Rorug03G0130100 Rorug03G0271600 Rorug04G0106200 Rorug07G0092400
rosa_samantha	Rh1BG150300 Rh1DG182200 Rh2AG068600 Rh2BG067800 Rh2BG264700 Rh2BG278600 Rh2CG069100 Rh4CG322900 Rh5BG390400 Rh7AG223300 Rh7BG218400 Rh7CG236600 Rh7DG230300 Rh7DG231100
rosa_wichuraiana	Rw4G029570 Rw7G019260 Rw7G019350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	426
AccB1I	GGYRCC	1 cut(s)	426
AccB7I	CCANNNNNTGG	1 cut(s)	406
AccII	CGCG	6 cut(s)	78, 276, 300, 386, 432, 447
AclWI	GGATC	5 cut(s)	531, 900, 913, 950, 963
AcsI	RAATTY	2 cut(s)	661, 691
AfaI	GTAC	3 cut(s)	428, 840, 1081
AfiI	CCNNNNNNNGG	6 cut(s)	247, 321, 406, 805, 874, 915
AflII	CTTAAG	1 cut(s)	1073
AgsI	TTSAA	1 cut(s)	856
AjiI	CACGTC	2 cut(s)	92, 331
AjnI	CCWGG	4 cut(s)	15, 26, 240, 1037
AluBI	AGCT	2 cut(s)	415, 1072
AluI	AGCT	2 cut(s)	415, 1072
Alw26I	GTCTC	2 cut(s)	825, 1058
AlwI	GGATC	5 cut(s)	531, 900, 913, 950, 963
Ama87I	CYCGRG	1 cut(s)	460
AoxI	GGCC	4 cut(s)	34, 249, 339, 1086
ApeKI	GCWGC	1 cut(s)	388
ApoI	RAATTY	2 cut(s)	661, 691
ArsI	GACNNNNNNTTYG	2 cut(s)	269, 301
Asp718I	GGTACC	1 cut(s)	426
AspLEI	GCGC	6 cut(s)	80, 302, 388, 434, 449, 861
AspS9I	GGNCC	5 cut(s)	35, 220, 237, 250, 325
AsuC2I	CCSGG	5 cut(s)	48, 218, 461, 462, 521
AsuHPI	GGTGA	6 cut(s)	97, 196, 557, 586, 617, 698
AvaI	CYCGRG	1 cut(s)	460
AvaII	GGWCC	3 cut(s)	220, 237, 325
BamHI	GGATCC	2 cut(s)	905, 955
BanI	GGYRCC	1 cut(s)	426
BbsI	GAAGAC	2 cut(s)	386, 1155
BbvI	GCAGC	1 cut(s)	375
BccI	CCATC	9 cut(s)	400, 502, 608, 878, 934, 1006, 1021, 1054, 1147
BciT130I	CCWGG	4 cut(s)	17, 28, 242, 1039
BcnI	CCSGG	5 cut(s)	48, 218, 461, 462, 521
BcoDI	GTCTC	2 cut(s)	825, 1058
BfaI	CTAG	3 cut(s)	641, 666, 753
BfmI	CTRYAG	2 cut(s)	389, 676
BfrI	CTTAAG	1 cut(s)	1073
BisI	GCNGC	8 cut(s)	71, 298, 315, 348, 351, 374, 389, 397
BlsI	GCNGC	8 cut(s)	72, 299, 316, 349, 352, 375, 390, 398
Bme1390I	CCNGG	9 cut(s)	17, 28, 48, 218, 242, 461, 462, 521, 1039
Bme18I	GGWCC	3 cut(s)	220, 237, 325
BmeT110I	CYCGRG	1 cut(s)	460
BmgBI	CACGTC	2 cut(s)	92, 331
BmgT120I	GGNCC	5 cut(s)	35, 220, 237, 250, 325
BmiI	GGNNCC	7 cut(s)	221, 222, 239, 326, 428, 907, 957
BmrFI	CCNGG	9 cut(s)	17, 28, 48, 218, 242, 461, 462, 521, 1039
BmrI	ACTGGG	2 cut(s)	331, 837
BmsI	GCATC	1 cut(s)	1108
BmuI	ACTGGG	2 cut(s)	331, 837
BpiI	GAAGAC	2 cut(s)	386, 1155
BpmI	CTGGAG	1 cut(s)	1088
BpuMI	CCSGG	5 cut(s)	48, 218, 461, 462, 521
BsaBI	GATNNNNATC	2 cut(s)	777, 954
BsaJI	CCNNGG	5 cut(s)	15, 240, 336, 460, 1038
BsaXI	ACNNNNNCTCC	2 cut(s)	160, 190
Bsc4I	CCNNNNNNNGG	6 cut(s)	247, 321, 406, 805, 874, 915
Bse1I	ACTGG	2 cut(s)	326, 843
Bse3DI	GCAATG	1 cut(s)	868
Bse8I	GATNNNNATC	2 cut(s)	777, 954
BseBI	CCWGG	4 cut(s)	17, 28, 242, 1039
BseDI	CCNNGG	5 cut(s)	15, 240, 336, 460, 1038
BseGI	GGATG	7 cut(s)	114, 261, 508, 945, 1017, 1065, 1123
BseJI	GATNNNNATC	2 cut(s)	777, 954
BseLI	CCNNNNNNNGG	6 cut(s)	247, 321, 406, 805, 874, 915
BseMI	GCAATG	1 cut(s)	868
BseMII	CTCAG	3 cut(s)	426, 699, 1074
BseNI	ACTGG	2 cut(s)	326, 843
BseRI	GAGGAG	6 cut(s)	142, 145, 349, 352, 355, 358
BseXI	GCAGC	1 cut(s)	375
Bsh1236I	CGCG	6 cut(s)	78, 276, 300, 386, 432, 447
Bsh1285I	CGRYCG	1 cut(s)	280
BshFI	GGCC	4 cut(s)	36, 251, 341, 1088
BshNI	GGYRCC	1 cut(s)	426
BsiEI	CGRYCG	1 cut(s)	280
BsiHKCI	CYCGRG	1 cut(s)	460
BsiSI	CCGG	4 cut(s)	47, 217, 461, 521
BslFI	GGGAC	4 cut(s)	67, 206, 338, 821
BslI	CCNNNNNNNGG	6 cut(s)	247, 321, 406, 805, 874, 915
BsmAI	GTCTC	2 cut(s)	825, 1058
BsmBI	CGTCTC	1 cut(s)	1058
BsmFI	GGGAC	4 cut(s)	67, 206, 338, 821
BsnI	GGCC	4 cut(s)	36, 251, 341, 1088
BsoBI	CYCGRG	1 cut(s)	460
Bsp143I	GATC	4 cut(s)	523, 637, 905, 955
BspANI	GGCC	4 cut(s)	36, 251, 341, 1088
BspCNI	CTCAG	3 cut(s)	427, 700, 1075
BspFNI	CGCG	6 cut(s)	78, 276, 300, 386, 432, 447
BspLI	GGNNCC	7 cut(s)	221, 222, 239, 326, 428, 907, 957
BspMAI	CTGCAG	1 cut(s)	393
BspPI	GGATC	5 cut(s)	531, 900, 913, 950, 963
BspT107I	GGYRCC	1 cut(s)	426
BspTI	CTTAAG	1 cut(s)	1073
BsrDI	GCAATG	1 cut(s)	868
BsrI	ACTGG	2 cut(s)	326, 843
BssECI	CCNNGG	5 cut(s)	15, 240, 336, 460, 1038
BssMI	GATC	4 cut(s)	523, 637, 905, 955
BssT1I	CCWWGG	1 cut(s)	336
Bst2UI	CCWGG	4 cut(s)	17, 28, 242, 1039
Bst4CI	ACNGT	2 cut(s)	199, 791
Bst6I	CTCTTC	2 cut(s)	917, 1046
BstAFI	CTTAAG	1 cut(s)	1073
BstC8I	GCNNGC	2 cut(s)	22, 422
BstDEI	CTNAG	4 cut(s)	435, 708, 1083, 1173
BstF5I	GGATG	7 cut(s)	114, 261, 508, 945, 1017, 1065, 1123
BstFNI	CGCG	6 cut(s)	78, 276, 300, 386, 432, 447
BstHHI	GCGC	6 cut(s)	80, 302, 388, 434, 449, 861
BstKTI	GATC	4 cut(s)	526, 640, 908, 958
BstMAI	GTCTC	2 cut(s)	825, 1058
BstMBI	GATC	4 cut(s)	523, 637, 905, 955
BstMCI	CGRYCG	1 cut(s)	280
BstMWI	GCNNNNNNNGC	4 cut(s)	84, 347, 421, 971
BstNI	CCWGG	4 cut(s)	17, 28, 242, 1039
BstSCI	CCNGG	9 cut(s)	15, 26, 46, 216, 240, 459, 460, 519, 1037
BstSFI	CTRYAG	2 cut(s)	389, 676
BstUI	CGCG	6 cut(s)	78, 276, 300, 386, 432, 447
BstV1I	GCAGC	1 cut(s)	375
BstV2I	GAAGAC	2 cut(s)	386, 1155
BstX2I	RGATCY	2 cut(s)	905, 955
BstYI	RGATCY	2 cut(s)	905, 955
BsuRI	GGCC	4 cut(s)	36, 251, 341, 1088
BtgZI	GCGATG	1 cut(s)	1166
BtrI	CACGTC	2 cut(s)	92, 331
BtsCI	GGATG	7 cut(s)	114, 261, 508, 945, 1017, 1065, 1123
BtsI	GCAGTG	1 cut(s)	398
BtsIMutI	CAGTG	2 cut(s)	398, 850
Cac8I	GCNNGC	2 cut(s)	22, 422
CfoI	GCGC	6 cut(s)	80, 302, 388, 434, 449, 861
Cfr13I	GGNCC	5 cut(s)	35, 220, 237, 250, 325
Cfr9I	CCCGGG	1 cut(s)	460
Csp6I	GTAC	3 cut(s)	427, 839, 1080
CviAII	CATG	4 cut(s)	187, 499, 1090, 1169
CviQI	GTAC	3 cut(s)	427, 839, 1080
DdeI	CTNAG	4 cut(s)	435, 708, 1083, 1173
DpnI	GATC	4 cut(s)	525, 639, 907, 957
DpnII	GATC	4 cut(s)	523, 637, 905, 955
Eam1104I	CTCTTC	2 cut(s)	917, 1046
EarI	CTCTTC	2 cut(s)	917, 1046
EciI	GGCGGA	2 cut(s)	284, 1072
Eco130I	CCWWGG	1 cut(s)	336
Eco47I	GGWCC	3 cut(s)	220, 237, 325
Eco88I	CYCGRG	1 cut(s)	460
EcoO109I	RGGNCCY	1 cut(s)	220
EcoRI	GAATTC	2 cut(s)	661, 691
EcoRII	CCWGG	4 cut(s)	15, 26, 240, 1037
EcoT14I	CCWWGG	1 cut(s)	336
EcoT22I	ATGCAT	1 cut(s)	1123
ErhI	CCWWGG	1 cut(s)	336
Esp3I	CGTCTC	1 cut(s)	1058
FaeI	CATG	4 cut(s)	190, 502, 1093, 1172
FaqI	GGGAC	4 cut(s)	67, 206, 338, 821
FatI	CATG	4 cut(s)	186, 498, 1089, 1168
FauI	CCCGC	3 cut(s)	92, 221, 728
Fnu4HI	GCNGC	8 cut(s)	71, 298, 315, 348, 351, 374, 389, 397
FokI	GGATG	7 cut(s)	101, 248, 515, 952, 1024, 1072, 1130
Fsp4HI	GCNGC	8 cut(s)	71, 298, 315, 348, 351, 374, 389, 397
FspBI	CTAG	3 cut(s)	641, 666, 753
GlaI	GCGC	6 cut(s)	79, 301, 387, 433, 448, 860
GluI	GCNGC	8 cut(s)	71, 298, 315, 348, 351, 374, 389, 397
GsuI	CTGGAG	1 cut(s)	1088
HaeIII	GGCC	4 cut(s)	36, 251, 341, 1088
HapII	CCGG	4 cut(s)	47, 217, 461, 521
HhaI	GCGC	6 cut(s)	80, 302, 388, 434, 449, 861
Hin1II	CATG	4 cut(s)	190, 502, 1093, 1172
Hin6I	GCGC	6 cut(s)	78, 300, 386, 432, 447, 859
HinP1I	GCGC	6 cut(s)	78, 300, 386, 432, 447, 859
HindIII	AAGCTT	1 cut(s)	413
HinfI	GANTC	5 cut(s)	55, 512, 778, 1097, 1160
HpaII	CCGG	4 cut(s)	47, 217, 461, 521
HphI	GGTGA	6 cut(s)	97, 196, 557, 586, 617, 698
Hpy166II	GTNNAC	1 cut(s)	192
Hpy188I	TCNGA	4 cut(s)	517, 528, 660, 709
Hpy188III	TCNNGA	2 cut(s)	747, 903
Hpy8I	GTNNAC	1 cut(s)	192
Hpy99I	CGWCG	3 cut(s)	203, 335, 481
HpyAV	CCTTC	3 cut(s)	121, 753, 1141
HpyCH4III	ACNGT	2 cut(s)	199, 791
HpyCH4IV	ACGT	2 cut(s)	91, 330
HpyCH4V	TGCA	3 cut(s)	391, 965, 1121
HpyF10VI	GCNNNNNNNGC	4 cut(s)	84, 347, 421, 971
HpyF3I	CTNAG	4 cut(s)	435, 708, 1083, 1173
HpySE526I	ACGT	2 cut(s)	91, 330
Hsp92II	CATG	4 cut(s)	190, 502, 1093, 1172
HspAI	GCGC	6 cut(s)	78, 300, 386, 432, 447, 859
KflI	GGGWCCC	1 cut(s)	220
KpnI	GGTACC	1 cut(s)	430
Kzo9I	GATC	4 cut(s)	523, 637, 905, 955
LmnI	GCTCC	1 cut(s)	1069
Lsp1109I	GCAGC	1 cut(s)	375
LweI	GCATC	1 cut(s)	1108
MaeI	CTAG	3 cut(s)	641, 666, 753
MaeII	ACGT	2 cut(s)	91, 330
MaeIII	GTNAC	1 cut(s)	202
MalI	GATC	4 cut(s)	525, 639, 907, 957
MboI	GATC	4 cut(s)	523, 637, 905, 955
MboII	GAAGA	9 cut(s)	391, 463, 931, 934, 1036, 1063, 1147, 1150, 1155
MflI	RGATCY	2 cut(s)	905, 955
MluCI	AATT	6 cut(s)	568, 618, 650, 661, 691, 816
MlyI	GAGTC	3 cut(s)	49, 521, 1169
MmeI	TCCRAC	4 cut(s)	203, 477, 540, 1107
Mph1103I	ATGCAT	1 cut(s)	1123
MseI	TTAA	5 cut(s)	672, 726, 783, 795, 1074
MspA1I	CMGCKG	1 cut(s)	282
MspCI	CTTAAG	1 cut(s)	1073
MspI	CCGG	4 cut(s)	47, 217, 461, 521
MspR9I	CCNGG	9 cut(s)	17, 28, 48, 218, 242, 461, 462, 521, 1039
MvaI	CCWGG	4 cut(s)	17, 28, 242, 1039
MvnI	CGCG	6 cut(s)	78, 276, 300, 386, 432, 447
MwoI	GCNNNNNNNGC	4 cut(s)	84, 347, 421, 971
NciI	CCSGG	5 cut(s)	48, 218, 461, 462, 521
NdeII	GATC	4 cut(s)	523, 637, 905, 955
NlaIII	CATG	4 cut(s)	190, 502, 1093, 1172
NlaIV	GGNNCC	7 cut(s)	221, 222, 239, 326, 428, 907, 957
NmuCI	GTSAC	1 cut(s)	202
NsiI	ATGCAT	1 cut(s)	1123
PcsI	WCGNNNNNNNCGW	1 cut(s)	273
PfeI	GAWTC	2 cut(s)	778, 1097
PflFI	GACNNNGTC	1 cut(s)	329
PflMI	CCANNNNNTGG	1 cut(s)	406
PkrI	GCNGC	8 cut(s)	72, 299, 316, 349, 352, 375, 390, 398
PleI	GAGTC	3 cut(s)	49, 520, 1168
PpsI	GAGTC	3 cut(s)	49, 520, 1168
PpuMI	RGGWCCY	1 cut(s)	220
Psp5II	RGGWCCY	1 cut(s)	220
Psp6I	CCWGG	4 cut(s)	15, 26, 240, 1037
PspGI	CCWGG	4 cut(s)	15, 26, 240, 1037
PspN4I	GGNNCC	7 cut(s)	221, 222, 239, 326, 428, 907, 957
PspPI	GGNCC	5 cut(s)	35, 220, 237, 250, 325
PspPPI	RGGWCCY	1 cut(s)	220
PstI	CTGCAG	1 cut(s)	393
PsuI	RGATCY	2 cut(s)	905, 955
PsyI	GACNNNGTC	1 cut(s)	329
RsaI	GTAC	3 cut(s)	428, 840, 1081
RsaNI	GTAC	3 cut(s)	427, 839, 1080
SaqAI	TTAA	5 cut(s)	672, 726, 783, 795, 1074
SatI	GCNGC	8 cut(s)	71, 298, 315, 348, 351, 374, 389, 397
Sau3AI	GATC	4 cut(s)	523, 637, 905, 955
Sau96I	GGNCC	5 cut(s)	35, 220, 237, 250, 325
SchI	GAGTC	3 cut(s)	49, 521, 1169
ScrFI	CCNGG	9 cut(s)	17, 28, 48, 218, 242, 461, 462, 521, 1039
SetI	ASST	9 cut(s)	94, 105, 176, 333, 417, 559, 671, 1040, 1074
SfaNI	GCATC	1 cut(s)	1108
SfcI	CTRYAG	2 cut(s)	389, 676
SinI	GGWCC	3 cut(s)	220, 237, 325
SmaI	CCCGGG	1 cut(s)	462
SmlI	CTYRAG	1 cut(s)	1073
SmoI	CTYRAG	1 cut(s)	1073
Sse9I	AATT	6 cut(s)	568, 618, 650, 661, 691, 816
SspMI	CTAG	3 cut(s)	641, 666, 753
StyD4I	CCNGG	9 cut(s)	15, 26, 46, 216, 240, 459, 460, 519, 1037
StyI	CCWWGG	1 cut(s)	336
TaaI	ACNGT	2 cut(s)	199, 791
TaiI	ACGT	2 cut(s)	94, 333
TaqI	TCGA	3 cut(s)	730, 746, 980
TasI	AATT	6 cut(s)	568, 618, 650, 661, 691, 816
TauI	GCSGC	7 cut(s)	73, 300, 317, 350, 353, 376, 399
TfiI	GAWTC	2 cut(s)	778, 1097
Tru1I	TTAA	5 cut(s)	672, 726, 783, 795, 1074
Tru9I	TTAA	5 cut(s)	672, 726, 783, 795, 1074
TscAI	CASTG	2 cut(s)	398, 850
TseFI	GTSAC	1 cut(s)	202
TseI	GCWGC	1 cut(s)	388
Tsp45I	GTSAC	1 cut(s)	202
TspDTI	ATGAA	5 cut(s)	103, 482, 663, 1037, 1106
TspGWI	ACGGA	1 cut(s)	1082
TspMI	CCCGGG	1 cut(s)	460
TspRI	CASTG	2 cut(s)	398, 850
Tth111I	GACNNNGTC	1 cut(s)	329
Van91I	CCANNNNNTGG	1 cut(s)	406
Vha464I	CTTAAG	1 cut(s)	1073
VpaK11BI	GGWCC	3 cut(s)	220, 237, 325
XapI	RAATTY	2 cut(s)	661, 691
XcmI	CCANNNNNNNNNTGG	1 cut(s)	231
XmaI	CCCGGG	1 cut(s)	460
XspI	CTAG	3 cut(s)	641, 666, 753
Zsp2I	ATGCAT	1 cut(s)	1123

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.