FvH4_6g48220

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000363
Physical Location & Seq
Reverse (-)
36363280 .. 36366073
2794 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g48220.t1

Sequence Viewer

Length: 2727 bp
ATGGGTAACATCTTCTCAGTCAACTCATCATGTGATGCCACTGCTTCTCGCTTCTTGGGTTCAATATTCCGACGAGCAGCATATGTTGGTAAGCTCGAAATAAACCTTCGACATCTGAAAACTTCCTTGGAAGAATTGAAGTGTTTACAGAATGACGTGAAGAGAAGGGTTGCGGTTGCTGAGCAGCAGCCACACATGAAGCAGTTAGACCAAGTACAGAATTGGATTTCAAGGGTGAACTCTCTTGAGGCTCGAGTAAGGGAAGCTGTCGACAGTAGCAGTCAACAAACGGAGAGTTTGTGTTGTGGAGGGTATTGTTCAAAGAGCTACATGTCAAGCTACAAGTATGGAACGAAGGTGGCTAAACTGTTGGTGGAAGTCGCTGACTTAAAGAGCAAGGGACTTTTTCCAGAGGTGGCTGAAAAATTGCCTCAACCTATAATCAATGAAAGACCTCTTGAGCCAACTGTGGGATTGGATTCATTGCTTGACAAGGTTTGGAGCCACGTTGAAGATGTGGAGGTGGGAGTACTTGGCTTATATGGGATGGGAGGGGTCGGCAAGACCACCCTCCTTACCCAAATCCATAACAAATTCCAACACACTGATTTTGATGTTGCAATCTGGATAGTAGTCTCCAAGGACCTCAGAGTCGAAGCAATTCAAAATAGAATTGGAGAAAAGATTGGTTTGAGAGACCCTACATGGGAGCTTAAAGATCCAGATGAAAAAGCCGATGCAATCTCAAGCCTCCTGAGCAGAAAAAAGTTTGTGTTGTTCTTGGATGATCTATGGGAGAGAGTTGATATAACCAAAATCGGAGTGCCGTTTCCTGATCAACACAACAAGTCCAAGGTTATCTTCACAACTCGTTCTGAGGATGTGTGTGGTTTTATGGGTGCTCAACAAAAGATTCTTGTGGAGTGTTTGCCATGGGACAAAGCGTGGGACTTGTTTCAGGAGAAGACTGGCAGAGATGCACTTCAAATTCATGCCGATATTCCTAAACTTGCTGAAGCTGTTGCAGCAGAGTGTGGCGGTTTGCCTCTTGCACTAACCACAGTTGGTCGAGTGATGTCCACTAAGAAGACTCCGCAAGAATGGAATCATGCGATCAGAGTGTTGAAGAAAGCTAGCTTTGAGTTTTCAGGTATGGGAGACCAGGTGTTCCCTCTATTGAAATTCAGTTACGATAATTTCCCTAGTGACAAAGTCAGAGCATGCTTCCTGTATTGTGCTTTATTTTCTGAAGACTACGATATACCTAAAGATGATTTAGTATGGTATTGGATGTGTGAAAATATGTTAGATGAACATAGTCATCCGGAGGAAGCACGAGATGAGAGCTACCATATCATAGGTACTCTTCTCAATGTATGTATGCTAGAAGAGGGTAAAGACGGTTGTGTAAAAATGCACGATGTTGTTCGTGACATGGCATTGTGGTTGGCTTGTGATCCAAAGAAAGCAGATGATAGTTTTCTTGTGCATACAGGGGCCGATTTGATTGAAGCACCAATTGCCAGAAAATGGAAGAACTCGAAAAGGGTATCATTGATGGCTAACCACATCAAAGAATTGGTTGAAACACCAAACTCTCCATATCTGTTGACTTTATTTCTCAGGAGAAATCATCTGCAGAAGATTGCTACAGGCTTCTTTAACTCTATGTCTAATCTGCTAGTTTTAGATCTGTCTTCAAATAAGGATCTCGCTCAACTGCCCGTTGGAGTTTCAAGCTTGGTTTCACTACACCATCTCAATTTGTCGAACACTGGTATAAAAGAGTTGCCGACAGAGTTAAAGTGCTTAGAGAGGCTGGAATATTTGAATTTTGAGAATACACCGAATCTTTATTCTCTTCCACCAACCCTAATATCAAGTTTTCGGATGCTGAAAGTTCTGAGAATGGTACATTCTGGTCCTGATTCTGAGGATCAAAATCATTTTGATGATGAAAAAGCGATGGTAAAGGAATTGCATGGTCTGAAGCACCTGGACTACTTGACTTTGGACATTAGAAGCACCTCTTGTTTCCAAAACTTTATCAGCTGCAAATTACTCTCTTGCTGCACTCGACTTCTATATCTCCTGGGTTATGACAATCATTCGAGCTCTCTAGATATATCATCCATGGGGAAGAAACACCTTGAACAGCTTAACATTAGGGGTTATCCTAATTTGGAAGATGTAGTACTGGACATTGATTGGGCTGGAGGACTAGCAGGTAATAATCTTCGGAACGCAATAATGAGAATCCACAACTGCTTCCATGGCCTTCAAATGATAATGATACAATACTGTGACAATCTCAAGGACCTGACACAGCTTTGTTTTGCTCCAAATCTCATATCTGTATATGTACTTTGCTGCTCTAGAATGGAGACAATAATCGATATAAAAAAACTTGGTGGAGATGCAAATGTGAGAAAAGAATTCAATCTGTTTGCGAAAATGGGACATCTGGATGTTAGATGGCTACCCGCGTTGAAGAGTATATACGGAGCTACCTTGCCCTTTCCATGCCTAAGCTACATCAACGTAGATGGATGTCCAGAACTGAAGAAACTGCCACTCGACTCTAGCAGTGCTCAAGGAGGAACCAATCTCACCCTTAGAGGAGAGCAGGACTGGTGGGATGGGTTACAGTGGGATGACCAACTCGCATTAGATACCTTTCGTCCCAGCTACATCTGTTGGAATGTAGATCAATACTTGCAAACTTAA

Protein Analysis

909

Amino Acids

102.82

Weight (kDa)

5.94

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 161 - 326 9.7e-48 NB-ARC domain
WHD_DRP PF23559 414 - 480 5.9e-09 Disease resistance protein Winged helix domain
LRR_14 PF23598 536 - 725 4.8e-12 Leucine-rich repeat region
LRR_8 PF13855 536 - 593 7.5e-08 Leucine rich repeat
LRR_RPS2 PF23247 754 - 857 6.4e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12210 AT1G12210 AT1G12210 AT1G12220 AT1G12220 AT1G12220 AT1G12280 AT1G12280 AT1G12290 AT1G12290 AT1G12290 AT1G12290 AT1G15890 AT1G15890 AT1G51480 AT1G52660 AT1G52660 AT1G61180 AT1G61180 AT1G61190 AT1G61190 AT1G61190 AT1G61190 AT1G61300 AT1G61300 AT1G61310 AT3G15700 AT3G15700 AT4G10780 AT4G10780 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43740 AT5G43740 AT5G63020
fragaria_vesca FvH4_6g30050 FvH4_6g48220
malus_domestica MD09G1055800.v1.1 MD09G1198800.v1.1 MD17G1204800.v1.1 MD17G1204900.v1.1 MD17G1205000.v1.1 MD17G1205300.v1.1 MD17G1205700.v1.1 MD17G1205800.v1.1 MD17G1224100.v1.1
prunus_persica Prupe.3G054800_v2.0.a1 Prupe.3G054800_v2.0.a1 Prupe.3G080300_v2.0.a1 Prupe.3G080400_v2.0.a1 Prupe.3G080500_v2.0.a1 Prupe.3G080600_v2.0.a1 Prupe.3G080800_v2.0.a1 Prupe.3G083200_v2.0.a1 Prupe.3G099800_v2.0.a1 Prupe.3G099800_v2.0.a1
pyrus_communis pycom17g20860 pycom17g20910 pycom17g22790
rosa_chinensis RchiOBHm_Chr2g0136891 RchiOBHm_Chr2g0136901 RchiOBHm_Chr2g0167711 RchiOBHm_Chr2g0167721 RchiOBHm_Chr2g0167731 RchiOBHm_Chr4g0407611
rosa_laevigata RLG00000018943 RLG00000019278 RLG00000019282 RLG00000019587 RLG00000021748 RLG00000021749 RLG00000031057
rosa_multiflora Rmu_sc0002143.1_g000003 Rmu_sc0002782.1_g000001 Rmu_sc0023879.1_g000001 Rmu_sc0042171.1_g000001
rosa_roxburghii Rroxscaffold_2G00083300 Rroxscaffold_2G00107890 Rroxscaffold_2G00116680
rosa_rugosa Rorug02G0271700 Rorug02G0334900 Rorug02G0531800 Rorug02G0531800
rosa_samantha Rh2AG359900 Rh2AG385100 Rh2AG600500 Rh2AG600600 Rh2BG392200 Rh2BG611000 Rh2BG611100 Rh2BG611200 Rh2CG343100 Rh2CG343300 Rh2CG372300 Rh2CG581400 Rh2CG581500 Rh2DG382900 Rh2DG623700 Rh5AG027500 Rh5CG029900
rosa_wichuraiana Rw2G031490 Rw2G049850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 650
Acc36I ACCTGC 1 cut(s) 2216
AccB7I CCANNNNNTGG 1 cut(s) 1530
AccI GTMKAC 1 cut(s) 270
AccII CGCG 1 cut(s) 2486
AccIII TCCGGA 1 cut(s) 1324
AciI CCGC 4 cut(s) 173, 1038, 1094, 2484
AclWI GGATC 4 cut(s) 713, 1451, 1716, 1944
AcsI RAATTY 5 cut(s) 593, 987, 1181, 1831, 2435
AcuI CTGAAG 4 cut(s) 1035, 1269, 2009, 2582
AfaI GTAC 6 cut(s) 216, 531, 1363, 1914, 2196, 2364
AfiI CCNNNNNNNGG 3 cut(s) 470, 706, 1530
AflIII ACRYGT 1 cut(s) 330
AjiI CACGTC 1 cut(s) 157
AjnI CCWGG 3 cut(s) 1161, 1995, 2091
AjuI GAANNNNNNNTTGG 4 cut(s) 110, 142, 845, 877
Alw21I GWGCWC 3 cut(s) 904, 2117, 2593
Alw26I GTCTC 4 cut(s) 640, 690, 1152, 2378
AlwI GGATC 4 cut(s) 713, 1451, 1716, 1944
Ama87I CYCGRG 1 cut(s) 252
Aor13HI TCCGGA 1 cut(s) 1324
AoxI GGCC 2 cut(s) 1497, 2275
ApeKI GCWGC 7 cut(s) 77, 184, 187, 1025, 2052, 2070, 2370
ApoI RAATTY 5 cut(s) 593, 987, 1181, 1831, 2435
Asp700I GAANNNNTTC 1 cut(s) 660
AspS9I GGNCC 4 cut(s) 643, 1497, 1922, 2317
AsuHPI GGTGA 2 cut(s) 247, 2602
AsuNHI GCTAGC 1 cut(s) 1133
AvaI CYCGRG 1 cut(s) 252
AvaII GGWCC 3 cut(s) 643, 1922, 2317
BanII GRGCYC 1 cut(s) 2117
BarI GAAGNNNNNNTAC 4 cut(s) 2178, 2210, 2483, 2515
BauI CACGAG 1 cut(s) 1335
BbsI GAAGAC 4 cut(s) 971, 1094, 1257, 1689
Bbv12I GWGCWC 3 cut(s) 904, 2117, 2593
BbvI GCAGC 7 cut(s) 89, 196, 199, 1037, 2039, 2057, 2357
BccI CCATC 7 cut(s) 541, 1552, 1764, 1960, 2469, 2540, 2633
BceAI ACGGC 1 cut(s) 811
BciT130I CCWGG 3 cut(s) 1163, 1997, 2093
BclI TGATCA 1 cut(s) 835
BcoDI GTCTC 4 cut(s) 640, 690, 1152, 2378
BfaI CTAG 8 cut(s) 1134, 1203, 1385, 1682, 2120, 2222, 2376, 2583
BfmI CTRYAG 2 cut(s) 1637, 1650
BfuAI ACCTGC 1 cut(s) 2216
BglII AGATCT 1 cut(s) 1690
BisI GCNGC 7 cut(s) 78, 185, 188, 1026, 2053, 2071, 2371
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 7 cut(s) 79, 186, 189, 1027, 2054, 2072, 2372
BmcAI AGTACT 2 cut(s) 531, 2196
Bme1390I CCNGG 3 cut(s) 1163, 1997, 2093
Bme18I GGWCC 3 cut(s) 643, 1922, 2317
BmeT110I CYCGRG 1 cut(s) 252
BmgBI CACGTC 1 cut(s) 157
BmgT120I GGNCC 4 cut(s) 643, 1497, 1922, 2317
BmiI GGNNCC 3 cut(s) 503, 1498, 2602
BmrFI CCNGG 3 cut(s) 1163, 1997, 2093
BmsI GCATC 5 cut(s) 25, 727, 967, 1881, 2407
BmtI GCTAGC 1 cut(s) 1137
BpiI GAAGAC 4 cut(s) 971, 1094, 1257, 1689
BpmI CTGGAG 1 cut(s) 2235
Bpu10I CCTNAGC 2 cut(s) 755, 2528
Bpu1102I GCTNAGC 1 cut(s) 180
BpuEI CTTGAG 5 cut(s) 266, 479, 730, 2297, 2577
Bsa29I ATCGAT 1 cut(s) 2394
BsaBI GATNNNNATC 1 cut(s) 1941
BsaI GGTCTC 2 cut(s) 690, 1152
BsaJI CCNNGG 7 cut(s) 126, 639, 852, 932, 2092, 2133, 2272
BsaWI WCCGGW 1 cut(s) 1324
BsaXI ACNNNNNCTCC 4 cut(s) 284, 314, 1149, 1179
Bsc4I CCNNNNNNNGG 3 cut(s) 470, 706, 1530
Bse1I ACTGG 4 cut(s) 973, 1780, 2202, 2636
Bse3DI GCAATG 1 cut(s) 482
Bse8I GATNNNNATC 1 cut(s) 1941
BseAI TCCGGA 1 cut(s) 1324
BseBI CCWGG 3 cut(s) 1163, 1997, 2093
BseCI ATCGAT 1 cut(s) 2394
BseDI CCNNGG 7 cut(s) 126, 639, 852, 932, 2092, 2133, 2272
BseJI GATNNNNATC 1 cut(s) 1941
BseLI CCNNNNNNNGG 3 cut(s) 470, 706, 1530
BseMI GCAATG 1 cut(s) 482
BseMII CTCAG 8 cut(s) 30, 171, 661, 746, 867, 1636, 1895, 1923
BseNI ACTGG 4 cut(s) 973, 1780, 2202, 2636
BseRI GAGGAG 1 cut(s) 2634
BseXI GCAGC 7 cut(s) 89, 196, 199, 1037, 2039, 2057, 2357
BseYI CCCAGC 1 cut(s) 2684
BsgI GTGCAG 1 cut(s) 2056
Bsh1236I CGCG 1 cut(s) 2486
BshFI GGCC 2 cut(s) 1499, 2277
BshVI ATCGAT 1 cut(s) 2394
BsiHKAI GWGCWC 3 cut(s) 904, 2117, 2593
BsiHKCI CYCGRG 1 cut(s) 252
BsiSI CCGG 1 cut(s) 1325
BslFI GGGAC 5 cut(s) 414, 950, 962, 2472, 2667
BslI CCNNNNNNNGG 3 cut(s) 470, 706, 1530
BsmAI GTCTC 4 cut(s) 640, 690, 1152, 2378
BsmFI GGGAC 5 cut(s) 414, 950, 962, 2472, 2667
BsnI GGCC 2 cut(s) 1499, 2277
Bso31I GGTCTC 2 cut(s) 690, 1152
BsoBI CYCGRG 1 cut(s) 252
Bsp1286I GDGCHC 3 cut(s) 904, 2117, 2593
Bsp13I TCCGGA 1 cut(s) 1324
Bsp143I GATC 9 cut(s) 718, 787, 835, 1113, 1456, 1690, 1708, 1936, 2707
Bsp1720I GCTNAGC 1 cut(s) 180
Bsp19I CCATGG 3 cut(s) 932, 2133, 2272
BspACI CCGC 4 cut(s) 173, 1038, 1094, 2484
BspANI GGCC 2 cut(s) 1499, 2277
BspCNI CTCAG 8 cut(s) 29, 172, 660, 747, 868, 1635, 1896, 1924
BspDI ATCGAT 1 cut(s) 2394
BspEI TCCGGA 1 cut(s) 1324
BspFNI CGCG 1 cut(s) 2486
BspLI GGNNCC 3 cut(s) 503, 1498, 2602
BspMAI CTGCAG 1 cut(s) 1641
BspMI ACCTGC 1 cut(s) 2216
BspOI GCTAGC 1 cut(s) 1137
BspPI GGATC 4 cut(s) 713, 1451, 1716, 1944
BspTNI GGTCTC 2 cut(s) 690, 1152
BsrDI GCAATG 1 cut(s) 482
BsrI ACTGG 4 cut(s) 973, 1780, 2202, 2636
BssECI CCNNGG 7 cut(s) 126, 639, 852, 932, 2092, 2133, 2272
BssMI GATC 9 cut(s) 718, 787, 835, 1113, 1456, 1690, 1708, 1936, 2707
BssSI CACGAG 1 cut(s) 1335
BssT1I CCWWGG 6 cut(s) 126, 639, 852, 932, 2133, 2272
Bst2BI CACGAG 1 cut(s) 1335
Bst2UI CCWGG 3 cut(s) 1163, 1997, 2093
Bst4CI ACNGT 7 cut(s) 275, 369, 469, 1063, 1403, 2303, 2649
Bst6I CTCTTC 5 cut(s) 155, 1371, 1383, 1866, 2486
BstAPI GCANNNNNTGC 1 cut(s) 1520
BstC8I GCNNGC 2 cut(s) 1135, 1222
BstDSI CCRYGG 3 cut(s) 932, 2133, 2272
BstFNI CGCG 1 cut(s) 2486
BstKTI GATC 9 cut(s) 721, 790, 838, 1116, 1459, 1693, 1711, 1939, 2710
BstMAI GTCTC 4 cut(s) 640, 690, 1152, 2378
BstMBI GATC 9 cut(s) 718, 787, 835, 1113, 1456, 1690, 1708, 1936, 2707
BstMWI GCNNNNNNNGC 4 cut(s) 756, 1025, 1520, 2274
BstNI CCWGG 3 cut(s) 1163, 1997, 2093
BstNSI RCATGY 2 cut(s) 334, 1224
BstSCI CCNGG 3 cut(s) 1161, 1995, 2091
BstSFI CTRYAG 2 cut(s) 1637, 1650
BstUI CGCG 1 cut(s) 2486
BstV1I GCAGC 7 cut(s) 89, 196, 199, 1037, 2039, 2057, 2357
BstV2I GAAGAC 4 cut(s) 971, 1094, 1257, 1689
BstX2I RGATCY 3 cut(s) 718, 1690, 1708
BstYI RGATCY 3 cut(s) 718, 1690, 1708
Bsu15I ATCGAT 1 cut(s) 2394
BsuRI GGCC 2 cut(s) 1499, 2277
BsuTUI ATCGAT 1 cut(s) 2394
BtgI CCRYGG 3 cut(s) 932, 2133, 2272
BtgZI GCGATG 1 cut(s) 1979
BtrI CACGTC 1 cut(s) 157
BtsI GCAGTG 2 cut(s) 39, 2593
BtsIMutI CAGTG 5 cut(s) 39, 603, 1773, 2593, 2654
BveI ACCTGC 1 cut(s) 2216
Cac8I GCNNGC 2 cut(s) 1135, 1222
Cfr13I GGNCC 4 cut(s) 643, 1497, 1922, 2317
ClaI ATCGAT 1 cut(s) 2394
CsiI ACCWGGT 1 cut(s) 1161
Csp6I GTAC 6 cut(s) 215, 530, 1362, 1913, 2195, 2363
CviQI GTAC 6 cut(s) 215, 530, 1362, 1913, 2195, 2363
DpnI GATC 9 cut(s) 720, 789, 837, 1115, 1458, 1692, 1710, 1938, 2709
DpnII GATC 9 cut(s) 718, 787, 835, 1113, 1456, 1690, 1708, 1936, 2707
DrdI GACNNNNNNGTC 1 cut(s) 650
DseDI GACNNNNNNGTC 1 cut(s) 650
Eam1104I CTCTTC 5 cut(s) 155, 1371, 1383, 1866, 2486
EarI CTCTTC 5 cut(s) 155, 1371, 1383, 1866, 2486
Ecl136II GAGCTC 1 cut(s) 2115
Eco130I CCWWGG 6 cut(s) 126, 639, 852, 932, 2133, 2272
Eco24I GRGCYC 1 cut(s) 2117
Eco31I GGTCTC 2 cut(s) 690, 1152
Eco47I GGWCC 3 cut(s) 643, 1922, 2317
Eco53kI GAGCTC 1 cut(s) 2115
Eco57I CTGAAG 4 cut(s) 1035, 1269, 2009, 2582
Eco88I CYCGRG 1 cut(s) 252
EcoICRI GAGCTC 1 cut(s) 2115
EcoO109I RGGNCCY 2 cut(s) 643, 2317
EcoRI GAATTC 1 cut(s) 2435
EcoRII CCWGG 3 cut(s) 1161, 1995, 2091
EcoT14I CCWWGG 6 cut(s) 126, 639, 852, 932, 2133, 2272
EcoT38I GRGCYC 1 cut(s) 2117
ErhI CCWWGG 6 cut(s) 126, 639, 852, 932, 2133, 2272
FalI AAGNNNNNCTT 4 cut(s) 845, 877, 2014, 2046
FaqI GGGAC 5 cut(s) 414, 950, 962, 2472, 2667
FauI CCCGC 1 cut(s) 2491
FauNDI CATATG 1 cut(s) 82
FbaI TGATCA 1 cut(s) 835
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 7 cut(s) 78, 185, 188, 1026, 2053, 2071, 2371
FriOI GRGCYC 1 cut(s) 2117
Fsp4HI GCNGC 7 cut(s) 78, 185, 188, 1026, 2053, 2071, 2371
FspBI CTAG 8 cut(s) 1134, 1203, 1385, 1682, 2120, 2222, 2376, 2583
GluI GCNGC 7 cut(s) 78, 185, 188, 1026, 2053, 2071, 2371
GsaI CCCAGC 1 cut(s) 2688
GsuI CTGGAG 1 cut(s) 2235
HaeIII GGCC 2 cut(s) 1499, 2277
HapII CCGG 1 cut(s) 1325
HincII GTYRAC 4 cut(s) 22, 271, 284, 1611
HindII GTYRAC 4 cut(s) 22, 271, 284, 1611
HindIII AAGCTT 1 cut(s) 1738
HinfI GANTC 9 cut(s) 479, 651, 913, 1090, 1105, 1849, 1928, 2256, 2579
HpaII CCGG 1 cut(s) 1325
HphI GGTGA 2 cut(s) 247, 2602
Hpy166II GTNNAC 7 cut(s) 22, 146, 238, 271, 284, 1080, 1611
Hpy8I GTNNAC 7 cut(s) 22, 146, 238, 271, 284, 1080, 1611
Hpy99I CGWCG 1 cut(s) 75
HpyAV CCTTC 4 cut(s) 116, 159, 349, 2288
HpyCH4III ACNGT 7 cut(s) 275, 369, 469, 1063, 1403, 2303, 2649
HpyCH4IV ACGT 3 cut(s) 156, 507, 2541
HpyF10VI GCNNNNNNNGC 4 cut(s) 756, 1025, 1520, 2274
HpySE526I ACGT 3 cut(s) 156, 507, 2541
Kpn2I TCCGGA 1 cut(s) 1324
Ksp22I TGATCA 1 cut(s) 835
Kzo9I GATC 9 cut(s) 718, 787, 835, 1113, 1456, 1690, 1708, 1936, 2707
LmnI GCTCC 4 cut(s) 501, 709, 2344, 2504
Lsp1109I GCAGC 7 cut(s) 89, 196, 199, 1037, 2039, 2057, 2357
LweI GCATC 5 cut(s) 25, 727, 967, 1881, 2407
MabI ACCWGGT 1 cut(s) 1161
MaeI CTAG 8 cut(s) 1134, 1203, 1385, 1682, 2120, 2222, 2376, 2583
MaeII ACGT 3 cut(s) 156, 507, 2541
MaeIII GTNAC 6 cut(s) 5, 1187, 1205, 1430, 2303, 2643
MalI GATC 9 cut(s) 720, 789, 837, 1115, 1458, 1692, 1710, 1938, 2709
MboI GATC 9 cut(s) 718, 787, 835, 1113, 1456, 1690, 1708, 1936, 2707
MfeI CAATTG 1 cut(s) 1518
MflI RGATCY 3 cut(s) 718, 1690, 1708
MhlI GDGCHC 3 cut(s) 904, 2117, 2593
MlyI GAGTC 3 cut(s) 660, 1084, 2573
MmeI TCCRAC 4 cut(s) 94, 622, 1708, 2678
MroI TCCGGA 1 cut(s) 1324
MroXI GAANNNNTTC 1 cut(s) 660
MseI TTAA 6 cut(s) 389, 714, 1662, 1802, 2160, 2725
MslI CAYNNNNRTG 2 cut(s) 1950, 2466
MspA1I CMGCKG 1 cut(s) 2052
MspI CCGG 1 cut(s) 1325
MspR9I CCNGG 3 cut(s) 1163, 1997, 2093
MunI CAATTG 1 cut(s) 1518
MvaI CCWGG 3 cut(s) 1163, 1997, 2093
MvnI CGCG 1 cut(s) 2486
MwoI GCNNNNNNNGC 4 cut(s) 756, 1025, 1520, 2274
NcoI CCATGG 3 cut(s) 932, 2133, 2272
NdeI CATATG 1 cut(s) 82
NdeII GATC 9 cut(s) 718, 787, 835, 1113, 1456, 1690, 1708, 1936, 2707
NheI GCTAGC 1 cut(s) 1133
NlaIV GGNNCC 3 cut(s) 503, 1498, 2602
NmuCI GTSAC 3 cut(s) 1205, 1430, 2303
NspI RCATGY 2 cut(s) 334, 1224
PaeI GCATGC 1 cut(s) 1224
PaeR7I CTCGAG 1 cut(s) 252
PciI ACATGT 1 cut(s) 330
PdmI GAANNNNTTC 1 cut(s) 660
PfeI GAWTC 6 cut(s) 479, 913, 1105, 1849, 1928, 2256
PflFI GACNNNGTC 1 cut(s) 1211
PflMI CCANNNNNTGG 1 cut(s) 1530
PkrI GCNGC 7 cut(s) 79, 186, 189, 1027, 2054, 2072, 2372
PleI GAGTC 3 cut(s) 659, 1084, 2573
PpsI GAGTC 3 cut(s) 659, 1084, 2573
PpuMI RGGWCCY 2 cut(s) 643, 2317
PscI ACATGT 1 cut(s) 330
Psp124BI GAGCTC 1 cut(s) 2117
Psp5II RGGWCCY 2 cut(s) 643, 2317
Psp6I CCWGG 3 cut(s) 1161, 1995, 2091
PspFI CCCAGC 1 cut(s) 2684
PspGI CCWGG 3 cut(s) 1161, 1995, 2091
PspN4I GGNNCC 3 cut(s) 503, 1498, 2602
PspPI GGNCC 4 cut(s) 643, 1497, 1922, 2317
PspPPI RGGWCCY 2 cut(s) 643, 2317
PspXI VCTCGAGB 1 cut(s) 252
PstI CTGCAG 1 cut(s) 1641
PsuI RGATCY 3 cut(s) 718, 1690, 1708
PsyI GACNNNGTC 1 cut(s) 1211
PvuII CAGCTG 1 cut(s) 2052
RsaI GTAC 6 cut(s) 216, 531, 1363, 1914, 2196, 2364
RsaNI GTAC 6 cut(s) 215, 530, 1362, 1913, 2195, 2363
RseI CAYNNNNRTG 2 cut(s) 1950, 2466
SacI GAGCTC 1 cut(s) 2117
SalI GTCGAC 1 cut(s) 269
SaqAI TTAA 6 cut(s) 389, 714, 1662, 1802, 2160, 2725
SatI GCNGC 7 cut(s) 78, 185, 188, 1026, 2053, 2071, 2371
Sau3AI GATC 9 cut(s) 718, 787, 835, 1113, 1456, 1690, 1708, 1936, 2707
Sau96I GGNCC 4 cut(s) 643, 1497, 1922, 2317
ScaI AGTACT 2 cut(s) 531, 2196
SchI GAGTC 3 cut(s) 660, 1084, 2573
ScrFI CCNGG 3 cut(s) 1163, 1997, 2093
SduI GDGCHC 3 cut(s) 904, 2117, 2593
SexAI ACCWGGT 1 cut(s) 1161
SfaNI GCATC 5 cut(s) 25, 727, 967, 1881, 2407
SfcI CTRYAG 2 cut(s) 1637, 1650
Sfr274I CTCGAG 1 cut(s) 252
SinI GGWCC 3 cut(s) 643, 1922, 2317
SlaI CTCGAG 1 cut(s) 252
SmiMI CAYNNNNRTG 2 cut(s) 1950, 2466
SmlI CTYRAG 6 cut(s) 245, 252, 458, 745, 2312, 2592
SmoI CTYRAG 6 cut(s) 245, 252, 458, 745, 2312, 2592
SphI GCATGC 1 cut(s) 1224
SsiI CCGC 4 cut(s) 173, 1038, 1094, 2484
SspI AATATT 2 cut(s) 66, 1826
SspMI CTAG 8 cut(s) 1134, 1203, 1385, 1682, 2120, 2222, 2376, 2583
SstI GAGCTC 1 cut(s) 2117
StyD4I CCNGG 3 cut(s) 1161, 1995, 2091
StyI CCWWGG 6 cut(s) 126, 639, 852, 932, 2133, 2272
TaaI ACNGT 7 cut(s) 275, 369, 469, 1063, 1403, 2303, 2649
TaiI ACGT 3 cut(s) 159, 510, 2544
TatI WGTACW 4 cut(s) 214, 529, 2194, 2362
TfiI GAWTC 6 cut(s) 479, 913, 1105, 1849, 1928, 2256
Tru1I TTAA 6 cut(s) 389, 714, 1662, 1802, 2160, 2725
Tru9I TTAA 6 cut(s) 389, 714, 1662, 1802, 2160, 2725
TscAI CASTG 5 cut(s) 46, 610, 1780, 2593, 2654
TseFI GTSAC 3 cut(s) 1205, 1430, 2303
TseI GCWGC 7 cut(s) 77, 184, 187, 1025, 2052, 2070, 2370
Tsp45I GTSAC 3 cut(s) 1205, 1430, 2303
TspDTI ATGAA 7 cut(s) 212, 462, 471, 741, 980, 1326, 1971
TspGWI ACGGA 2 cut(s) 305, 2517
TspRI CASTG 5 cut(s) 46, 610, 1780, 2593, 2654
Tth111I GACNNNGTC 1 cut(s) 1211
Van91I CCANNNNNTGG 1 cut(s) 1530
VpaK11BI GGWCC 3 cut(s) 643, 1922, 2317
XapI RAATTY 5 cut(s) 593, 987, 1181, 1831, 2435
XbaI TCTAGA 2 cut(s) 2119, 2375
XceI RCATGY 2 cut(s) 334, 1224
XhoI CTCGAG 1 cut(s) 252
XmiI GTMKAC 1 cut(s) 270
XmnI GAANNNNTTC 1 cut(s) 660
XspI CTAG 8 cut(s) 1134, 1203, 1385, 1682, 2120, 2222, 2376, 2583
ZrmI AGTACT 2 cut(s) 531, 2196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.