MD09G1198800.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000363
Physical Location & Seq
Reverse (-)
18140162 .. 18142879
2718 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1198800.v1.1.491

Sequence Viewer

Length: 2436 bp
ATGGGCAACATCTTTTCAATCTCCATATCATGTGATGACATACTTTCTCGCTGCTGGGATTCCACAGTTGGACAAATAGCATACCCTTGTATGTTCAAGTCCCACCTTCATGATCTGCAGACTGCTTTAGAAGAATTAACGGATCTAAAGAACGATTTGAAGAGAAGGGTTGACAATGCCGAGCAGCAGCAACATTTGAAACGCTTAGACCAGGTACAGAGATGGATAACAAGGGTGGAAGCCATGGAAGCTCAAGCCAATAACTTGGTGAACGGGAAAGATATCAGAAGTCAAAAAGTTGAGAAATTACGTGATTCTGGAGGTGGGTTGTTATGTAATTGCAAAACCCACTACAAGTTGGGGAAAAAATTGGCTAAGATGTTGATGGAAGTGAGTGCTTTACAGAGCAGGGGAGCTTTTGAAGTTGTGGCTGAACGGGTACCTGCAGCTATGGTGTATGAAAGAAGTATTGAACCAACTGTGGGCATGGAGTCAACATTTGGCAAGGTCTGGGATTGCACTGAAGATGAGCAAGTGGGAATTATTGGCTTGTATGGGATGGGAGGTGTAGGTAAGACCACCCTTTTGACCAAGATCAACAACAACTTCCTTCATACCCCCAATGATTTTGATCTTGTGATTTGGATAGAGGTCTCTAAGGATCTAAAGCTTGAAAATATTCAAGACAGCATTGGGGAAAAGATAGGGAGTTGCGATGGCTCGTGGAAGGATAAAGATCACCTTAGAAAAGCTGAAGACATCTTTGCGGCTTTAAAATCGAAGAGATTTGTATTATTGTTGGATGATATATGGGAGAGGGTTGATGTAGCCAAAATCGGGGTTCCGATTCCTGACAGAGAGAATAAGTCTAAGCTTGTATTCACAACTCGCTCTGAGGAGGTTTGTAGCCGTATGGGTGCTCACAAAAAGATCAAGGTGGAGTGTTTGGCATGGGACAGAGCATGGACTTTGTTTCAAGAGAAGGTGGGTGAAGAAACACTTTATATTCATCCAGATATCCCTACACTAGCAGAGATGGTTGCCAAAGAATGTGACGGTTTGCCACTGGCTCTAATTACAGTTGGACGGGCCATGGCTAGCAAGAAGACACCCCAAGAATGGAATCATGCAATTCAAGTTTTAAAACGATCTGCCTCGGAATTTTCAGGTATGGGAGATGAGGTATTCCCTCTTTTGAAATTCAGTTATGATAATCTACCTAGCGAGAAAGCTAGATCTTGTTTCTTGTATTGTGCATTATTTCCGGAAGATTTTCTCATTCACAAAAGAAGATTAATATATTGTTGGGTTGGGGAGGGAATACTGGATGAGTATGATGACATGACTGGAGCTCAAAACCAAGGTTATGATATTATAGGTACTCTTGTTAATGCATGTTTATTGGAAGGTAAGGAAGATTATGTGAAAATGCACGATGTTCTTCGTGACATGGCATTGTGGTTGGCCTGTGAATGCGGGAAGGCCAAAGATAATTTCATAGTTCGTACTGGCGCTCATTTGATTAAAGCACCAGATTTGGAAAAATGGAAGGGTGTGAAAAGGATGTCACTAATGGCTAATCAGATTGAGAATCTGGTGGAAAGATCCACATGCCCCAGTTTATCCACCTTGTTTCTAACTGATAATCATTTGAAGATAATTGGTGAAGGCTTCTTTCAGCATATGCCCAGTTTAAGAGTTTTGGACCTGTCGGAAAACAAGGGTATAACACATTTGCCACCGGGAATATCAAAATTGAAGTCACTAAAATATCTCAATCTATCACAGACAGGTATAAGAGGGTTGCCTGTTGAGTTAAAGGCCTTAGACAAGCTAAAATATTTGAACCTAGAGTTTACCAGTAAGATGGATATTGTTCCGAAGAATGAAATTTCAAGTTTTCTGATGCTCAGAGTTTTGAGAATGTATGATTGTGGTTCTTCTGACGATATTCTGTTTGGTGGAGAAGAAGCTTTAGTAGAGGAATTGGTGTGCTTGAAACATTTGGATGTGATGACTATGACCATGAGGTGTGTCTCTGCTTTCGAAAGAATATGTACCTCCCTCAACTTACTAGCATGTACTCAAGTTCTATGCCTTGAGTCCTTCACCTCTCTGATTTCCCTTGATATATCGGCATTGGCAAATATGAAACACCTTGACATCCGTAATATTTGCGACTGTGAAAGCTTAGAAGATTTGAAAACTGATTTGTTATGGAATGGAGGGGCTGTACAAGTACCTAATGATCCTTGCAACTCGATCATCACGATCAAAAGTTTCTTCCACAGCCTTCAGCGGCTCCCACTGGATTCCAGTGTGAACAGACGGAATGTTGTCATCGAAGGAGAGGAAGAATGGTGGAATGGGTTGGAATGGGAGGATGGAGCTACTCGAAATGATTTTATTTCCTGCTTTAGAAATTTAAATATTTGA

Protein Analysis

812

Amino Acids

91.75

Weight (kDa)

5.61

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 166 - 333 3.9e-51 NB-ARC domain
WHD_DRP PF23559 420 - 485 7.9e-10 Disease resistance protein Winged helix domain
LRR_14 PF23598 523 - 728 6.1e-13 Leucine-rich repeat region
LRR_8 PF13855 539 - 581 5.5e-08 Leucine rich repeat
LRR_8 PF13855 568 - 619 2.1e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12210 AT1G12210 AT1G12210 AT1G12220 AT1G12220 AT1G12220 AT1G12280 AT1G12280 AT1G12290 AT1G12290 AT1G12290 AT1G12290 AT1G15890 AT1G15890 AT1G51480 AT1G52660 AT1G52660 AT1G61180 AT1G61180 AT1G61190 AT1G61190 AT1G61190 AT1G61190 AT1G61300 AT1G61300 AT1G61310 AT3G15700 AT3G15700 AT4G10780 AT4G10780 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43740 AT5G43740 AT5G63020
fragaria_vesca FvH4_6g30050 FvH4_6g48220
malus_domestica MD09G1055800.v1.1 MD09G1198800.v1.1 MD17G1204800.v1.1 MD17G1204900.v1.1 MD17G1205000.v1.1 MD17G1205300.v1.1 MD17G1205700.v1.1 MD17G1205800.v1.1 MD17G1224100.v1.1
prunus_persica Prupe.3G054800_v2.0.a1 Prupe.3G054800_v2.0.a1 Prupe.3G080300_v2.0.a1 Prupe.3G080400_v2.0.a1 Prupe.3G080500_v2.0.a1 Prupe.3G080600_v2.0.a1 Prupe.3G080800_v2.0.a1 Prupe.3G083200_v2.0.a1 Prupe.3G099800_v2.0.a1 Prupe.3G099800_v2.0.a1
pyrus_communis pycom17g20860 pycom17g20910 pycom17g22790
rosa_chinensis RchiOBHm_Chr2g0136891 RchiOBHm_Chr2g0136901 RchiOBHm_Chr2g0167711 RchiOBHm_Chr2g0167721 RchiOBHm_Chr2g0167731 RchiOBHm_Chr4g0407611
rosa_laevigata RLG00000018943 RLG00000019278 RLG00000019282 RLG00000019587 RLG00000021748 RLG00000021749 RLG00000031057
rosa_multiflora Rmu_sc0002143.1_g000003 Rmu_sc0002782.1_g000001 Rmu_sc0023879.1_g000001 Rmu_sc0042171.1_g000001
rosa_roxburghii Rroxscaffold_2G00083300 Rroxscaffold_2G00107890 Rroxscaffold_2G00116680
rosa_rugosa Rorug02G0271700 Rorug02G0334900 Rorug02G0531800 Rorug02G0531800
rosa_samantha Rh2AG359900 Rh2AG385100 Rh2AG600500 Rh2AG600600 Rh2BG392200 Rh2BG611000 Rh2BG611100 Rh2BG611200 Rh2CG343100 Rh2CG343300 Rh2CG372300 Rh2CG581400 Rh2CG581500 Rh2DG382900 Rh2DG623700 Rh5AG027500 Rh5CG029900
rosa_wichuraiana Rw2G031490 Rw2G049850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 451
Acc65I GGTACC 1 cut(s) 439
AccB1I GGYRCC 1 cut(s) 439
AccIII TCCGGA 1 cut(s) 1264
AciI CCGC 3 cut(s) 767, 1476, 2299
AclWI GGATC 4 cut(s) 150, 669, 1599, 2243
AcsI RAATTY 4 cut(s) 1160, 1199, 1890, 2422
AcuI CTGAAG 3 cut(s) 543, 774, 2279
AfaI GTAC 8 cut(s) 216, 441, 1381, 1507, 2059, 2083, 2235, 2241
AfiI CCNNNNNNNGG 3 cut(s) 482, 837, 1119
AjnI CCWGG 1 cut(s) 210
AjuI GAANNNNNNNTTGG 2 cut(s) 1478, 1510
Alw21I GWGCWC 2 cut(s) 922, 1354
Alw26I GTCTC 2 cut(s) 658, 2041
AlwI GGATC 4 cut(s) 150, 669, 1599, 2243
Aor13HI TCCGGA 1 cut(s) 1264
AoxI GGCC 4 cut(s) 1089, 1464, 1482, 1821
ApeKI GCWGC 4 cut(s) 51, 184, 187, 446
ApoI RAATTY 4 cut(s) 1160, 1199, 1890, 2422
ArsI GACNNNNNNTTYG 4 cut(s) 568, 600, 1745, 1777
AseI ATTAAT 1 cut(s) 1295
Asp700I GAANNNNTTC 2 cut(s) 678, 1272
Asp718I GGTACC 1 cut(s) 439
AspLEI GCGC 1 cut(s) 1514
AspS9I GGNCC 2 cut(s) 1089, 1705
AsuC2I CCSGG 1 cut(s) 1743
AsuHPI GGTGA 5 cut(s) 280, 731, 1001, 1676, 2101
AsuII TTCGAA 1 cut(s) 2046
AsuNHI GCTAGC 1 cut(s) 1097
AvaII GGWCC 1 cut(s) 1705
BaeI ACNNNNGTAYC 2 cut(s) 1371, 1404
BanI GGYRCC 1 cut(s) 439
BanII GRGCYC 1 cut(s) 1354
BauI CACGAG 1 cut(s) 721
BbsI GAAGAC 2 cut(s) 762, 1112
Bbv12I GWGCWC 2 cut(s) 922, 1354
BbvI GCAGC 4 cut(s) 38, 196, 199, 458
BccI CCATC 7 cut(s) 216, 379, 553, 710, 1030, 1861, 2378
BceAI ACGGC 1 cut(s) 894
BciT130I CCWGG 1 cut(s) 212
BcnI CCSGG 1 cut(s) 1743
BcoDI GTCTC 2 cut(s) 658, 2041
BfaI CTAG 6 cut(s) 1028, 1098, 1221, 1233, 1850, 2075
BfmI CTRYAG 2 cut(s) 116, 444
BfoI RGCGCY 1 cut(s) 1515
BfuAI ACCTGC 1 cut(s) 451
BglII AGATCT 1 cut(s) 1235
BisI GCNGC 6 cut(s) 52, 185, 188, 447, 768, 2300
BlsI GCNGC 6 cut(s) 53, 186, 189, 448, 769, 2301
Bme1390I CCNGG 2 cut(s) 212, 1743
Bme18I GGWCC 1 cut(s) 1705
BmgT120I GGNCC 2 cut(s) 1089, 1705
BmiI GGNNCC 3 cut(s) 441, 843, 2303
BmrFI CCNGG 2 cut(s) 212, 1743
BmrI ACTGGG 2 cut(s) 1611, 1683
BmsI GCATC 1 cut(s) 1896
BmtI GCTAGC 1 cut(s) 1101
BmuI ACTGGG 2 cut(s) 1611, 1683
BpiI GAAGAC 2 cut(s) 762, 1112
BpmI CTGGAG 2 cut(s) 339, 1368
Bpu14I TTCGAA 1 cut(s) 2046
BpuEI CTTGAG 3 cut(s) 237, 2070, 2120
BpuMI CCSGG 1 cut(s) 1743
BsaAI YACGTR 1 cut(s) 311
BsaBI GATNNNNATC 2 cut(s) 630, 735
BsaI GGTCTC 1 cut(s) 658
BsaJI CCNNGG 4 cut(s) 243, 1092, 1155, 1360
BsaWI WCCGGW 1 cut(s) 1264
BsaXI ACNNNNNCTCC 4 cut(s) 1167, 1197, 2217, 2247
Bsc4I CCNNNNNNNGG 3 cut(s) 482, 837, 1119
Bse1I ACTGG 9 cut(s) 1071, 1329, 1351, 1513, 1617, 1689, 1860, 2313, 2316
Bse8I GATNNNNATC 2 cut(s) 630, 735
BseAI TCCGGA 1 cut(s) 1264
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 4 cut(s) 243, 1092, 1155, 1360
BseGI GGATG 8 cut(s) 564, 808, 1009, 1333, 1569, 2014, 2163, 2389
BseJI GATNNNNATC 2 cut(s) 630, 735
BseLI CCNNNNNNNGG 3 cut(s) 482, 837, 1119
BseMII CTCAG 2 cut(s) 885, 1924
BseNI ACTGG 9 cut(s) 1071, 1329, 1351, 1513, 1617, 1689, 1860, 2313, 2316
BseRI GAGGAG 1 cut(s) 911
BseXI GCAGC 4 cut(s) 38, 196, 199, 458
BseYI CCCAGC 1 cut(s) 54
BshFI GGCC 4 cut(s) 1091, 1466, 1484, 1823
BshNI GGYRCC 1 cut(s) 439
BsiHKAI GWGCWC 2 cut(s) 922, 1354
BsiSI CCGG 2 cut(s) 1265, 1742
BslFI GGGAC 2 cut(s) 85, 968
BslI CCNNNNNNNGG 3 cut(s) 482, 837, 1119
BsmAI GTCTC 2 cut(s) 658, 2041
BsmFI GGGAC 2 cut(s) 85, 968
BsmI GAATGC 1 cut(s) 1478
BsnI GGCC 4 cut(s) 1091, 1466, 1484, 1823
Bso31I GGTCTC 1 cut(s) 658
Bsp119I TTCGAA 1 cut(s) 2046
Bsp1286I GDGCHC 2 cut(s) 922, 1354
Bsp13I TCCGGA 1 cut(s) 1264
Bsp1407I TGTACA 1 cut(s) 2233
Bsp19I CCATGG 2 cut(s) 243, 1092
BspACI CCGC 3 cut(s) 767, 1476, 2299
BspANI GGCC 4 cut(s) 1091, 1466, 1484, 1823
BspCNI CTCAG 2 cut(s) 886, 1923
BspEI TCCGGA 1 cut(s) 1264
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 3 cut(s) 441, 843, 2303
BspMAI CTGCAG 2 cut(s) 120, 448
BspMI ACCTGC 1 cut(s) 451
BspOI GCTAGC 1 cut(s) 1101
BspPI GGATC 4 cut(s) 150, 669, 1599, 2243
BspT104I TTCGAA 1 cut(s) 2046
BspT107I GGYRCC 1 cut(s) 439
BspTNI GGTCTC 1 cut(s) 658
BsrGI TGTACA 1 cut(s) 2233
BsrI ACTGG 9 cut(s) 1071, 1329, 1351, 1513, 1617, 1689, 1860, 2313, 2316
BssECI CCNNGG 4 cut(s) 243, 1092, 1155, 1360
BssSI CACGAG 1 cut(s) 721
BssT1I CCWWGG 3 cut(s) 243, 1092, 1360
Bst2BI CACGAG 1 cut(s) 721
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 5 cut(s) 67, 481, 1058, 1081, 2183
Bst6I CTCTTC 2 cut(s) 155, 776
BstAUI TGTACA 1 cut(s) 2233
BstBAI YACGTR 1 cut(s) 311
BstBI TTCGAA 1 cut(s) 2046
BstC8I GCNNGC 1 cut(s) 1099
BstDEI CTNAG 9 cut(s) 205, 375, 657, 743, 870, 894, 1825, 1910, 2191
BstDSI CCRYGG 2 cut(s) 243, 1092
BstF5I GGATG 8 cut(s) 564, 808, 1009, 1333, 1569, 2014, 2163, 2389
BstH2I RGCGCY 1 cut(s) 1515
BstHHI GCGC 1 cut(s) 1514
BstMAI GTCTC 2 cut(s) 658, 2041
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 3 cut(s) 1398, 1614, 2082
BstSCI CCNGG 2 cut(s) 210, 1741
BstSFI CTRYAG 2 cut(s) 116, 444
BstV1I GCAGC 4 cut(s) 38, 196, 199, 458
BstV2I GAAGAC 2 cut(s) 762, 1112
BstX2I RGATCY 4 cut(s) 142, 661, 1235, 1604
BstXI CCANNNNNNTGG 2 cut(s) 265, 1867
BstYI RGATCY 4 cut(s) 142, 661, 1235, 1604
BsuRI GGCC 4 cut(s) 1091, 1466, 1484, 1823
BtgI CCRYGG 2 cut(s) 243, 1092
BtgZI GCGATG 1 cut(s) 729
BtsCI GGATG 8 cut(s) 564, 808, 1009, 1333, 1569, 2014, 2163, 2389
BtsIMutI CAGTG 4 cut(s) 519, 1064, 2306, 2323
BveI ACCTGC 1 cut(s) 451
Cac8I GCNNGC 1 cut(s) 1099
CciI TCATGA 1 cut(s) 109
CfoI GCGC 1 cut(s) 1514
Cfr13I GGNCC 2 cut(s) 1089, 1705
CsiI ACCWGGT 1 cut(s) 210
Csp6I GTAC 8 cut(s) 215, 440, 1380, 1506, 2058, 2082, 2234, 2240
CviQI GTAC 8 cut(s) 215, 440, 1380, 1506, 2058, 2082, 2234, 2240
DdeI CTNAG 9 cut(s) 205, 375, 657, 743, 870, 894, 1825, 1910, 2191
DraI TTTAAA 3 cut(s) 774, 1143, 2427
Eam1104I CTCTTC 2 cut(s) 155, 776
EarI CTCTTC 2 cut(s) 155, 776
Ecl136II GAGCTC 1 cut(s) 1352
Eco130I CCWWGG 3 cut(s) 243, 1092, 1360
Eco147I AGGCCT 1 cut(s) 1823
Eco24I GRGCYC 1 cut(s) 1354
Eco31I GGTCTC 1 cut(s) 658
Eco32I GATATC 2 cut(s) 283, 1018
Eco47I GGWCC 1 cut(s) 1705
Eco53kI GAGCTC 1 cut(s) 1352
Eco57I CTGAAG 3 cut(s) 543, 774, 2279
EcoICRI GAGCTC 1 cut(s) 1352
EcoRII CCWGG 1 cut(s) 210
EcoRV GATATC 2 cut(s) 283, 1018
EcoT14I CCWWGG 3 cut(s) 243, 1092, 1360
EcoT22I ATGCAT 1 cut(s) 1396
EcoT38I GRGCYC 1 cut(s) 1354
ErhI CCWWGG 3 cut(s) 243, 1092, 1360
FalI AAGNNNNNCTT 4 cut(s) 726, 758, 984, 1016
FaqI GGGAC 2 cut(s) 85, 968
FauI CCCGC 1 cut(s) 1469
FauNDI CATATG 1 cut(s) 1683
Fnu4HI GCNGC 6 cut(s) 52, 185, 188, 447, 768, 2300
FokI GGATG 8 cut(s) 571, 815, 996, 1340, 1576, 2021, 2150, 2396
FriOI GRGCYC 1 cut(s) 1354
Fsp4HI GCNGC 6 cut(s) 52, 185, 188, 447, 768, 2300
FspBI CTAG 6 cut(s) 1028, 1098, 1221, 1233, 1850, 2075
GlaI GCGC 1 cut(s) 1513
GluI GCNGC 6 cut(s) 52, 185, 188, 447, 768, 2300
GsaI CCCAGC 1 cut(s) 58
GsuI CTGGAG 2 cut(s) 339, 1368
HaeII RGCGCY 1 cut(s) 1515
HaeIII GGCC 4 cut(s) 1091, 1466, 1484, 1823
HapII CCGG 2 cut(s) 1265, 1742
HhaI GCGC 1 cut(s) 1514
Hin6I GCGC 1 cut(s) 1512
HinP1I GCGC 1 cut(s) 1512
HincII GTYRAC 2 cut(s) 172, 495
HindII GTYRAC 2 cut(s) 172, 495
HindIII AAGCTT 4 cut(s) 668, 872, 1971, 2188
HinfI GANTC 8 cut(s) 59, 314, 491, 847, 1123, 1591, 2102, 2312
HpaII CCGG 2 cut(s) 1265, 1742
HphI GGTGA 5 cut(s) 280, 731, 1001, 1676, 2101
Hpy166II GTNNAC 5 cut(s) 172, 271, 495, 1857, 2323
Hpy188III TCNNGA 9 cut(s) 110, 318, 683, 851, 977, 1013, 1265, 1445, 2269
Hpy8I GTNNAC 5 cut(s) 172, 271, 495, 1857, 2323
HpyCH4III ACNGT 5 cut(s) 67, 481, 1058, 1081, 2183
HpyCH4IV ACGT 1 cut(s) 310
HpyCH4V TGCA 9 cut(s) 118, 342, 446, 519, 1130, 1256, 1394, 1432, 2256
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 9 cut(s) 205, 375, 657, 743, 870, 894, 1825, 1910, 2191
HpySE526I ACGT 1 cut(s) 310
HspAI GCGC 1 cut(s) 1512
Kpn2I TCCGGA 1 cut(s) 1264
KpnI GGTACC 1 cut(s) 443
LmnI GCTCC 4 cut(s) 413, 1349, 2307, 2387
Lsp1109I GCAGC 4 cut(s) 38, 196, 199, 458
LweI GCATC 1 cut(s) 1896
MabI ACCWGGT 1 cut(s) 210
MaeI CTAG 6 cut(s) 1028, 1098, 1221, 1233, 1850, 2075
MaeII ACGT 1 cut(s) 310
MaeIII GTNAC 4 cut(s) 1052, 1445, 1566, 1761
MflI RGATCY 4 cut(s) 142, 661, 1235, 1604
MhlI GDGCHC 2 cut(s) 922, 1354
MlyI GAGTC 2 cut(s) 500, 2111
MmeI TCCRAC 5 cut(s) 49, 780, 1063, 1692, 2352
Mph1103I ATGCAT 1 cut(s) 1396
MroI TCCGGA 1 cut(s) 1264
MroXI GAANNNNTTC 2 cut(s) 678, 1272
MseI TTAA 9 cut(s) 137, 773, 1142, 1295, 1389, 1524, 1694, 1817, 2426
MslI CAYNNNNRTG 2 cut(s) 108, 2007
MspA1I CMGCKG 1 cut(s) 2299
MspI CCGG 2 cut(s) 1265, 1742
MspR9I CCNGG 2 cut(s) 212, 1743
Mva1269I GAATGC 1 cut(s) 1478
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 248
NciI CCSGG 1 cut(s) 1743
NcoI CCATGG 2 cut(s) 243, 1092
NdeI CATATG 1 cut(s) 1683
NheI GCTAGC 1 cut(s) 1097
NlaIV GGNNCC 3 cut(s) 441, 843, 2303
NmeAIII GCCGAG 1 cut(s) 205
NmuCI GTSAC 4 cut(s) 1052, 1445, 1566, 1761
NsiI ATGCAT 1 cut(s) 1396
NspI RCATGY 3 cut(s) 1398, 1614, 2082
NspV TTCGAA 1 cut(s) 2046
PagI TCATGA 1 cut(s) 109
PceI AGGCCT 1 cut(s) 1823
PcsI WCGNNNNNNNCGW 1 cut(s) 2267
PctI GAATGC 1 cut(s) 1478
PdmI GAANNNNTTC 2 cut(s) 678, 1272
PfeI GAWTC 6 cut(s) 59, 314, 847, 1123, 1591, 2312
PkrI GCNGC 6 cut(s) 53, 186, 189, 448, 769, 2301
PleI GAGTC 2 cut(s) 499, 2110
PpsI GAGTC 2 cut(s) 499, 2110
Ppu21I YACGTR 1 cut(s) 311
PshBI ATTAAT 1 cut(s) 1295
Psp124BI GAGCTC 1 cut(s) 1354
Psp6I CCWGG 1 cut(s) 210
PspFI CCCAGC 1 cut(s) 54
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 3 cut(s) 441, 843, 2303
PspPI GGNCC 2 cut(s) 1089, 1705
PstI CTGCAG 2 cut(s) 120, 448
PsuI RGATCY 4 cut(s) 142, 661, 1235, 1604
RsaI GTAC 8 cut(s) 216, 441, 1381, 1507, 2059, 2083, 2235, 2241
RsaNI GTAC 8 cut(s) 215, 440, 1380, 1506, 2058, 2082, 2234, 2240
RseI CAYNNNNRTG 2 cut(s) 108, 2007
SacI GAGCTC 1 cut(s) 1354
SaqAI TTAA 9 cut(s) 137, 773, 1142, 1295, 1389, 1524, 1694, 1817, 2426
SatI GCNGC 6 cut(s) 52, 185, 188, 447, 768, 2300
Sau96I GGNCC 2 cut(s) 1089, 1705
SchI GAGTC 2 cut(s) 500, 2111
ScrFI CCNGG 2 cut(s) 212, 1743
SduI GDGCHC 2 cut(s) 922, 1354
SexAI ACCWGGT 1 cut(s) 210
SfaNI GCATC 1 cut(s) 1896
SfcI CTRYAG 2 cut(s) 116, 444
SfuI TTCGAA 1 cut(s) 2046
SinI GGWCC 1 cut(s) 1705
SmiI ATTTAAAT 1 cut(s) 2427
SmiMI CAYNNNNRTG 2 cut(s) 108, 2007
SmlI CTYRAG 3 cut(s) 252, 2085, 2099
SmoI CTYRAG 3 cut(s) 252, 2085, 2099
SseBI AGGCCT 1 cut(s) 1823
SsiI CCGC 3 cut(s) 767, 1476, 2299
SspI AATATT 4 cut(s) 679, 1841, 2173, 2431
SspMI CTAG 6 cut(s) 1028, 1098, 1221, 1233, 1850, 2075
SstI GAGCTC 1 cut(s) 1354
StuI AGGCCT 1 cut(s) 1823
StyD4I CCNGG 2 cut(s) 210, 1741
StyI CCWWGG 3 cut(s) 243, 1092, 1360
SwaI ATTTAAAT 1 cut(s) 2427
TaaI ACNGT 5 cut(s) 67, 481, 1058, 1081, 2183
TaiI ACGT 1 cut(s) 313
TaqI TCGA 5 cut(s) 779, 2046, 2261, 2343, 2395
TatI WGTACW 2 cut(s) 2081, 2233
TauI GCSGC 2 cut(s) 770, 2302
TfiI GAWTC 6 cut(s) 59, 314, 847, 1123, 1591, 2312
Tru1I TTAA 9 cut(s) 137, 773, 1142, 1295, 1389, 1524, 1694, 1817, 2426
Tru9I TTAA 9 cut(s) 137, 773, 1142, 1295, 1389, 1524, 1694, 1817, 2426
TscAI CASTG 4 cut(s) 526, 1071, 2313, 2323
TseFI GTSAC 4 cut(s) 1052, 1445, 1566, 1761
TseI GCWGC 4 cut(s) 51, 184, 187, 446
Tsp45I GTSAC 4 cut(s) 1052, 1445, 1566, 1761
TspDTI ATGAA 7 cut(s) 98, 474, 602, 998, 1486, 1902, 2165
TspGWI ACGGA 3 cut(s) 155, 2156, 2344
TspRI CASTG 4 cut(s) 526, 1071, 2313, 2323
VpaK11BI GGWCC 1 cut(s) 1705
VspI ATTAAT 1 cut(s) 1295
XapI RAATTY 4 cut(s) 1160, 1199, 1890, 2422
XceI RCATGY 3 cut(s) 1398, 1614, 2082
XmnI GAANNNNTTC 2 cut(s) 678, 1272
XspI CTAG 6 cut(s) 1028, 1098, 1221, 1233, 1850, 2075
Zsp2I ATGCAT 1 cut(s) 1396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.