Rh2CG581400

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000363
Physical Location & Seq
Reverse (-)
76009081 .. 76009902
822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG581400.1

Sequence Viewer

Length: 822 bp
ATGCTAAAAGTTCTGAGAATGGTACATTCTGGTACTTCTGATGATCGAAATCATTTTGATGATGGTGATGAAAAAGCGATGGTAGAGGAATTGCATGGTTTGAAACACCTGGACTACTTGACTCTGGACATTAGAAGTACCTCTTGTTTCCAAAACTTCGTCAGCAACAAATTAGCGACGTGCTGCACTCGAGCTCTACACCTCATGGGTTATGACAATCATTCGAGCTCTCTTGATATATCATCTGCGGAGACAAAACATCTTGAGCAGCTTGAGATTAGCGGCTATCCTAATATGGAAGATATAATAGTGGACATTGATTGGGCAGGAGGACGAGCATGTAACAATCCTCGGAACTCAATGATGAGAATCCAGAGATGCTTCCTTGGCCTTCAATACATAATGGTAAACGCATGTGCAGATCTCAAGGACTTGACACAGCTCTGTTTTGTTCCAAATCTCCTATCTGTTGCAGTATTCAAATGCTCTAGAATGGAGACAATAATCAATTTGAGTAAACTGGGTGGAGATGCAAATGTGGCAAAAGAATTTAACCTGTTTGCGAAACTGAAGCGTCTTGATTTGGCATTTCTACCAGCGTTGGAGAGCGTATACAAGACTGCCTTGCCCTTACCATGTCTAAAGTGGGTCAGCGTATATCAATGTCCAGCACTGAAGAAACTGCCACTCAACTCTAGCACTGCTCAAGGAGACGGATGCAATTTTAACCTAAGAGGAGAGCAGAAGTGGTGGGATGGGATAGAGTGGGAGGACCAACTTGCACTAGATACTATTCGTCCCTGCTACATGAGTTGGGATTGA

Protein Analysis

273

Amino Acids

30.9

Weight (kDa)

5.66

Isoelectric Point (pI)

32.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 124 - 228 6.3e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12210 AT1G12210 AT1G12210 AT1G12220 AT1G12220 AT1G12220 AT1G12280 AT1G12280 AT1G12290 AT1G12290 AT1G12290 AT1G12290 AT1G15890 AT1G15890 AT1G51480 AT1G52660 AT1G52660 AT1G61180 AT1G61180 AT1G61190 AT1G61190 AT1G61190 AT1G61190 AT1G61300 AT1G61300 AT1G61310 AT3G15700 AT3G15700 AT4G10780 AT4G10780 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43740 AT5G43740 AT5G63020
fragaria_vesca FvH4_6g30050 FvH4_6g48220
malus_domestica MD09G1055800.v1.1 MD09G1198800.v1.1 MD17G1204800.v1.1 MD17G1204900.v1.1 MD17G1205000.v1.1 MD17G1205300.v1.1 MD17G1205700.v1.1 MD17G1205800.v1.1 MD17G1224100.v1.1
prunus_persica Prupe.3G054800_v2.0.a1 Prupe.3G054800_v2.0.a1 Prupe.3G080300_v2.0.a1 Prupe.3G080400_v2.0.a1 Prupe.3G080500_v2.0.a1 Prupe.3G080600_v2.0.a1 Prupe.3G080800_v2.0.a1 Prupe.3G083200_v2.0.a1 Prupe.3G099800_v2.0.a1 Prupe.3G099800_v2.0.a1
pyrus_communis pycom17g20860 pycom17g20910 pycom17g22790
rosa_chinensis RchiOBHm_Chr2g0136891 RchiOBHm_Chr2g0136901 RchiOBHm_Chr2g0167711 RchiOBHm_Chr2g0167721 RchiOBHm_Chr2g0167731 RchiOBHm_Chr4g0407611
rosa_laevigata RLG00000018943 RLG00000019278 RLG00000019282 RLG00000019587 RLG00000021748 RLG00000021749 RLG00000031057
rosa_multiflora Rmu_sc0002143.1_g000003 Rmu_sc0002782.1_g000001 Rmu_sc0023879.1_g000001 Rmu_sc0042171.1_g000001
rosa_roxburghii Rroxscaffold_2G00083300 Rroxscaffold_2G00107890 Rroxscaffold_2G00116680
rosa_rugosa Rorug02G0271700 Rorug02G0334900 Rorug02G0531800 Rorug02G0531800
rosa_samantha Rh2AG359900 Rh2AG385100 Rh2AG600500 Rh2AG600600 Rh2BG392200 Rh2BG611000 Rh2BG611100 Rh2BG611200 Rh2CG343100 Rh2CG343300 Rh2CG372300 Rh2CG581400 Rh2CG581500 Rh2DG382900 Rh2DG623700 Rh5AG027500 Rh5CG029900
rosa_wichuraiana Rw2G031490 Rw2G049850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 612
AciI CCGC 2 cut(s) 248, 282
AcsI RAATTY 1 cut(s) 548
AcuI CTGAAG 2 cut(s) 590, 695
AfaI GTAC 3 cut(s) 24, 34, 139
AgsI TTSAA 3 cut(s) 103, 395, 481
AjiI CACGTC 1 cut(s) 180
AjnI CCWGG 1 cut(s) 108
AluBI AGCT 4 cut(s) 194, 228, 271, 442
AluI AGCT 4 cut(s) 194, 228, 271, 442
Alw21I GWGCWC 2 cut(s) 196, 230
Alw26I GTCTC 3 cut(s) 245, 491, 705
Ama87I CYCGRG 1 cut(s) 189
AoxI GGCC 1 cut(s) 388
ApeKI GCWGC 2 cut(s) 183, 268
ApoI RAATTY 1 cut(s) 548
AspS9I GGNCC 1 cut(s) 772
AsuHPI GGTGA 1 cut(s) 77
AvaI CYCGRG 1 cut(s) 189
AvaII GGWCC 1 cut(s) 772
BanII GRGCYC 2 cut(s) 196, 230
Bbv12I GWGCWC 2 cut(s) 196, 230
BbvI GCAGC 2 cut(s) 170, 280
BccI CCATC 3 cut(s) 56, 73, 749
BciT130I CCWGG 1 cut(s) 110
BcoDI GTCTC 3 cut(s) 245, 491, 705
BfaI CTAG 3 cut(s) 489, 696, 785
BglII AGATCT 1 cut(s) 421
BisI GCNGC 3 cut(s) 184, 269, 283
BlsI GCNGC 3 cut(s) 185, 270, 284
Bme1390I CCNGG 1 cut(s) 110
Bme18I GGWCC 1 cut(s) 772
BmeT110I CYCGRG 1 cut(s) 189
BmgBI CACGTC 1 cut(s) 180
BmgT120I GGNCC 1 cut(s) 772
BmrFI CCNGG 1 cut(s) 110
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 3 cut(s) 368, 520, 707
BmuI ACTGGG 1 cut(s) 530
BpuEI CTTGAG 4 cut(s) 284, 293, 410, 690
BsaBI GATNNNNATC 2 cut(s) 48, 368
BsaJI CCNNGG 2 cut(s) 350, 385
Bse1I ACTGG 1 cut(s) 525
Bse8I GATNNNNATC 2 cut(s) 48, 368
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 2 cut(s) 350, 385
BseGI GGATG 2 cut(s) 722, 760
BseJI GATNNNNATC 2 cut(s) 48, 368
BseMII CTCAG 1 cut(s) 5
BseNI ACTGG 1 cut(s) 525
BseRI GAGGAG 1 cut(s) 750
BseXI GCAGC 2 cut(s) 170, 280
BsgI GTGCAG 2 cut(s) 169, 438
BshFI GGCC 1 cut(s) 390
BsiHKAI GWGCWC 2 cut(s) 196, 230
BsiHKCI CYCGRG 1 cut(s) 189
BslFI GGGAC 1 cut(s) 783
BsmAI GTCTC 3 cut(s) 245, 491, 705
BsmBI CGTCTC 1 cut(s) 705
BsmFI GGGAC 1 cut(s) 783
BsnI GGCC 1 cut(s) 390
BsoBI CYCGRG 1 cut(s) 189
Bsp1286I GDGCHC 2 cut(s) 196, 230
Bsp143I GATC 2 cut(s) 43, 421
BspACI CCGC 2 cut(s) 248, 282
BspANI GGCC 1 cut(s) 390
BspCNI CTCAG 1 cut(s) 6
BsrI ACTGG 1 cut(s) 525
BssECI CCNNGG 2 cut(s) 350, 385
BssMI GATC 2 cut(s) 43, 421
BssNAI GTATAC 1 cut(s) 613
BssT1I CCWWGG 1 cut(s) 385
Bst1107I GTATAC 1 cut(s) 613
Bst2UI CCWGG 1 cut(s) 110
BstDEI CTNAG 2 cut(s) 14, 731
BstF5I GGATG 2 cut(s) 722, 760
BstKTI GATC 2 cut(s) 46, 424
BstMAI GTCTC 3 cut(s) 245, 491, 705
BstMBI GATC 2 cut(s) 43, 421
BstMWI GCNNNNNNNGC 2 cut(s) 387, 539
BstNI CCWGG 1 cut(s) 110
BstNSI RCATGY 2 cut(s) 342, 417
BstSCI CCNGG 1 cut(s) 108
BstV1I GCAGC 2 cut(s) 170, 280
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
BstZ17I GTATAC 1 cut(s) 613
BsuRI GGCC 1 cut(s) 390
BtgZI GCGATG 1 cut(s) 92
BtrI CACGTC 1 cut(s) 180
BtsCI GGATG 2 cut(s) 722, 760
BtsI GCAGTG 1 cut(s) 699
BtsIMutI CAGTG 2 cut(s) 671, 699
Cfr13I GGNCC 1 cut(s) 772
CseI GACGC 1 cut(s) 563
Csp6I GTAC 3 cut(s) 23, 33, 138
CviAII CATG 6 cut(s) 95, 205, 339, 414, 636, 808
CviJI RGCY 6 cut(s) 194, 228, 271, 285, 390, 442
CviKI_1 RGCY 6 cut(s) 194, 228, 271, 285, 390, 442
CviQI GTAC 3 cut(s) 23, 33, 138
DdeI CTNAG 2 cut(s) 14, 731
DpnI GATC 2 cut(s) 45, 423
DpnII GATC 2 cut(s) 43, 421
Ecl136II GAGCTC 2 cut(s) 194, 228
Eco130I CCWWGG 1 cut(s) 385
Eco24I GRGCYC 2 cut(s) 196, 230
Eco47I GGWCC 1 cut(s) 772
Eco53kI GAGCTC 2 cut(s) 194, 228
Eco57I CTGAAG 2 cut(s) 590, 695
Eco88I CYCGRG 1 cut(s) 189
EcoICRI GAGCTC 2 cut(s) 194, 228
EcoRII CCWGG 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 385
EcoT38I GRGCYC 2 cut(s) 196, 230
ErhI CCWWGG 1 cut(s) 385
Esp3I CGTCTC 1 cut(s) 705
FaeI CATG 6 cut(s) 98, 208, 342, 417, 639, 811
FalI AAGNNNNNCTT 4 cut(s) 127, 159, 608, 640
FaqI GGGAC 1 cut(s) 783
FatI CATG 6 cut(s) 94, 204, 338, 413, 635, 807
FblI GTMKAC 1 cut(s) 612
Fnu4HI GCNGC 3 cut(s) 184, 269, 283
FokI GGATG 2 cut(s) 729, 767
FriOI GRGCYC 2 cut(s) 196, 230
Fsp4HI GCNGC 3 cut(s) 184, 269, 283
FspBI CTAG 3 cut(s) 489, 696, 785
GluI GCNGC 3 cut(s) 184, 269, 283
HaeIII GGCC 1 cut(s) 390
HgaI GACGC 1 cut(s) 563
Hin1II CATG 6 cut(s) 98, 208, 342, 417, 639, 811
HinfI GANTC 2 cut(s) 121, 369
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 4 cut(s) 313, 409, 518, 613
Hpy188I TCNGA 3 cut(s) 15, 40, 354
Hpy188III TCNNGA 6 cut(s) 125, 233, 263, 373, 489, 578
Hpy8I GTNNAC 4 cut(s) 313, 409, 518, 613
Hpy99I CGWCG 1 cut(s) 181
HpyAV CCTTC 1 cut(s) 401
HpyCH4IV ACGT 1 cut(s) 179
HpyCH4V TGCA 7 cut(s) 94, 186, 419, 473, 533, 720, 782
HpyF10VI GCNNNNNNNGC 2 cut(s) 387, 539
HpyF3I CTNAG 2 cut(s) 14, 731
HpySE526I ACGT 1 cut(s) 179
Hsp92II CATG 6 cut(s) 98, 208, 342, 417, 639, 811
Kzo9I GATC 2 cut(s) 43, 421
Lsp1109I GCAGC 2 cut(s) 170, 280
LweI GCATC 3 cut(s) 368, 520, 707
MaeI CTAG 3 cut(s) 489, 696, 785
MaeII ACGT 1 cut(s) 179
MaeIII GTNAC 1 cut(s) 341
MalI GATC 2 cut(s) 45, 423
MboI GATC 2 cut(s) 43, 421
MboII GAAGA 2 cut(s) 311, 688
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 2 cut(s) 196, 230
MluCI AATT 5 cut(s) 89, 170, 508, 548, 721
MlyI GAGTC 1 cut(s) 115
MmeI TCCRAC 1 cut(s) 582
MnlI CCTC 7 cut(s) 79, 151, 212, 323, 360, 728, 763
MseI TTAA 2 cut(s) 552, 726
MslI CAYNNNNRTG 1 cut(s) 57
MspR9I CCNGG 1 cut(s) 110
MvaI CCWGG 1 cut(s) 110
MwoI GCNNNNNNNGC 2 cut(s) 387, 539
NdeII GATC 2 cut(s) 43, 421
NlaIII CATG 6 cut(s) 98, 208, 342, 417, 639, 811
NspI RCATGY 2 cut(s) 342, 417
PaeR7I CTCGAG 1 cut(s) 189
PfeI GAWTC 1 cut(s) 369
PkrI GCNGC 3 cut(s) 185, 270, 284
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
Psp124BI GAGCTC 2 cut(s) 196, 230
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PspPI GGNCC 1 cut(s) 772
PspXI VCTCGAGB 1 cut(s) 189
PsuI RGATCY 1 cut(s) 421
RsaI GTAC 3 cut(s) 24, 34, 139
RsaNI GTAC 3 cut(s) 23, 33, 138
RseI CAYNNNNRTG 1 cut(s) 57
SacI GAGCTC 2 cut(s) 196, 230
SaqAI TTAA 2 cut(s) 552, 726
SatI GCNGC 3 cut(s) 184, 269, 283
Sau3AI GATC 2 cut(s) 43, 421
Sau96I GGNCC 1 cut(s) 772
SchI GAGTC 1 cut(s) 115
ScrFI CCNGG 1 cut(s) 110
SduI GDGCHC 2 cut(s) 196, 230
SfaNI GCATC 3 cut(s) 368, 520, 707
Sfr274I CTCGAG 1 cut(s) 189
SinI GGWCC 1 cut(s) 772
SlaI CTCGAG 1 cut(s) 189
SmiMI CAYNNNNRTG 1 cut(s) 57
SmlI CTYRAG 5 cut(s) 189, 263, 272, 425, 705
SmoI CTYRAG 5 cut(s) 189, 263, 272, 425, 705
Sse9I AATT 5 cut(s) 89, 170, 508, 548, 721
SsiI CCGC 2 cut(s) 248, 282
SspMI CTAG 3 cut(s) 489, 696, 785
SstI GAGCTC 2 cut(s) 196, 230
StyD4I CCNGG 1 cut(s) 108
StyI CCWWGG 1 cut(s) 385
TaiI ACGT 1 cut(s) 182
TaqI TCGA 3 cut(s) 46, 190, 224
TasI AATT 5 cut(s) 89, 170, 508, 548, 721
TauI GCSGC 1 cut(s) 285
TfiI GAWTC 1 cut(s) 369
Tru1I TTAA 2 cut(s) 552, 726
Tru9I TTAA 2 cut(s) 552, 726
TscAI CASTG 2 cut(s) 678, 706
TseI GCWGC 2 cut(s) 183, 268
TspDTI ATGAA 1 cut(s) 84
TspGWI ACGGA 1 cut(s) 729
TspRI CASTG 2 cut(s) 678, 706
VpaK11BI GGWCC 1 cut(s) 772
XapI RAATTY 1 cut(s) 548
XbaI TCTAGA 1 cut(s) 488
XceI RCATGY 2 cut(s) 342, 417
XcmI CCANNNNNNNNNTGG 1 cut(s) 642
XhoI CTCGAG 1 cut(s) 189
XmiI GTMKAC 1 cut(s) 612
XspI CTAG 3 cut(s) 489, 696, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.