Rh2AG359900

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000363
Physical Location & Seq
Forward (+)
53339756 .. 53342452
2697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG359900.1

Sequence Viewer

Length: 2640 bp
ATGGGTAACTGCTGCTCGTCCCCTTTACCATCATGCGATCCCCTGATTACTCGATGTCTTGACTGCACAGTTGGACAAGCATCATACATGTGCTACATGGAAGACAACGTAGAGACTTTGAAAAGTGACATCCGACATTTACAGGCCTCAAAGAAGGACGTGCAGACGAAAGTTGATTTAGCAGAGCAGGAAGGACTGAAGCGTTTTGAGAGAGTTGGGTGGTGGATGTCAAGAGTGGAAGAGGTTGAAGTTGAAGTGAATGAATTGATAACAGCTAGCGGTGATGAAGTCAAGAAATTATGTTTAGGAGGATGTTGCTCAAGTGAATGCACGTCCAGCTACAAGTTTGGGAAACAAGTGGTTAGAAAGTTAAAAGAAGTCGATGCTTTAATGAAGTCTGTGCCAGCTGATGTAGCTGGGGAAGATGCGGCTTATCCGGACGATAGTAGGCCGACTGAGCCCACTGTCGGCATGCAATCCATGTTTGACAAGGTATGGAACCAGCTTCAAGAGCGCGAAGTGGGAATTGTTGGCTTATTTGGAATGGGAGGGGTGGGCAAGACTACTCTTCTGGACAAGATCTACAACACGTACCAAGATACCCCGAATGATTTTGATCACATAATCCGGGTAGTGGTCTCCAGGAACCACAAAATTGAAGACATTCAAAATGATATCGGAAGAAAAATTCGGTGTCTAAAAAGGAATAAAGAAGTCCACCAGCAAATGGATTCAACAATTCGATCTGCTTTGAGAGGCAAAAAATTTGTGTTGCTTTTGGATGATGTGTGGAGGCACATTGATCTGAAAAATGAAGTTGGAGTACCTGATCCTCATATTACAAACAGCAAAGTCATATTCACTACTCGTGATGAGGAGGTGTGCAACCAAATGGGTGGCAAGAAGCATAGGGTGAAGTGTTTGGCATGGGAGGACGCATGGAATTTGTTCAAGCAGAACTTCAACAAAGTGGAAGACATACTATGTCTTGAACCGGAAATTCCTCACTTGGCTGAATCTGTTGCTAAAAAGTGTGCCGGATTGCCTCTCGCGCTGATCATAGTCGGTAGGGCCATGTCTTGTAGGAAGACAGCAGGTGAATGGAGAGAAGCCATTCGTGATTTAAGAACCTCTGCTGGAAAATTTGAAGGTATGAGAGAGAAAGTCTTCCATCTTCTTAAGTTCAGTTATGACAACCTTGCTAGCAAGAAATTGAAGTCTTGTTTCTTATATTGTGCTTTATATCCGGAAGATTTTTCTATTCATAGAGATGAATTGATTTATAAGTGGATGGGTGAGGAAATGTTGGATGAGTATGCTGATGTAGCTGGAGCTCTAGACCAGGGTAACCGCATCATTGGTACTCTGGTTAATGCATGTTTATTAGATGATATTGGAGGTCATGTGAAAATGCATGATGTTATTCGTGATATGGCATTGTGGTTAGCTTGTCACTTGGAAGAAACAAAGGACAACTTCTTGGTTGAAGCAGGTGTTCTTTTGACTGAAGTGCCAATTGCAAAAATTGAAAAAGTTCAAGCTGTGAAAAGGGTGTCGTTGATGGCTAATGACATTGAAATTCTTGGGAAAATGCCTTCATGCTCCAATCTACTTACGTTGTTTCTTGGTAATAATCGTGTGAAGATAATTACTCAAGATTTCTTTGATTCTATGCTCAGTCTACAAGTTTTGGACTTGTCAAAGAACTTGAAGATAACCCAATTACCATCAGGAATATCAAGATTGGTTTCCTTAAAGTATCTTGATCTATCAGAAACAAGCATGAACGAGGTGCCAATTGAGTTCAAGGCTTTCGTAAAGCTAATATATCTCAACTTGGAGCATACCAGTCAACTTGATGTGATTCCACAAAATGTAATATCAAGCTTCTCAGAACTAAGAGCTTTGAGAATGTATAATTCTGGTGTTTGTAAGAGTGCTCTTGTTGGGCGCAATGAACACATTGAAAAGGAATTGCAGCTTTTGAAGCATTTGAGTATGTTGACATTCACCATACAGAGTGACTCAGGCTTTCAGAAGTTTGTCACCTCTTCCAAGTTACTGAGCTGCACTCAATTTCTATCCCTGGACGGTTTGAGACTTCTAGATATATCATCTCTGACTAAGATGAAGCGTCCATATTCTCTGGCCATTAGTGGCTGTGAAAGTATAGACTACAATTTGGGTGTCAGAAGCCAAAGTCGCTTTGGAAACCTTCAAGCTGTGAATATACATGATAGTCCTAACCTAAGAGACCTGACATGGTTGATTTTTGCCCCAGAGCTCAAGATTCTATCAGTAAGCTCGTGTTCTGAAATGGAAGAAATCATCCAACCTGACACATTTGGTGGGAGTGCTCAGGTACAAGAGATGAAACCATTTTCAAATTTGGTTGAATTGAAATTGATAAACTTACCACAAGTGAAGAGCATATGTGGAGAGAAAATCTTGCCTTTTCCATTCCTGAAGAGAATTGACGTCATTGACTGTCAAAATTTGAAGAAGCTCCCCCTCAACATAGACAGTGTTCCCGGCCGTAATGTTGTCATCACTGGAGAAGAAGACTGGTGGCATTTGTTAAATTGGGAGAATGAAGCTACTAAGGAAGCTTTTGGTCCCTGCTTTAGAGCACCAAACTAA

Protein Analysis

879

Amino Acids

99.28

Weight (kDa)

5.94

Isoelectric Point (pI)

50.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 159 - 321 2.1e-43 NB-ARC domain
WHD_DRP PF23559 414 - 479 6.6e-08 Disease resistance protein Winged helix domain
LRR_14 PF23598 518 - 777 1.4e-10 Leucine-rich repeat region
LRR_8 PF13855 536 - 592 6.9e-09 Leucine rich repeat
LRR_RPS2 PF23247 732 - 836 5.2e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12210 AT1G12210 AT1G12210 AT1G12220 AT1G12220 AT1G12220 AT1G12280 AT1G12280 AT1G12290 AT1G12290 AT1G12290 AT1G12290 AT1G15890 AT1G15890 AT1G51480 AT1G52660 AT1G52660 AT1G61180 AT1G61180 AT1G61190 AT1G61190 AT1G61190 AT1G61190 AT1G61300 AT1G61300 AT1G61310 AT3G15700 AT3G15700 AT4G10780 AT4G10780 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43740 AT5G43740 AT5G63020
fragaria_vesca FvH4_6g30050 FvH4_6g48220
malus_domestica MD09G1055800.v1.1 MD09G1198800.v1.1 MD17G1204800.v1.1 MD17G1204900.v1.1 MD17G1205000.v1.1 MD17G1205300.v1.1 MD17G1205700.v1.1 MD17G1205800.v1.1 MD17G1224100.v1.1
prunus_persica Prupe.3G054800_v2.0.a1 Prupe.3G054800_v2.0.a1 Prupe.3G080300_v2.0.a1 Prupe.3G080400_v2.0.a1 Prupe.3G080500_v2.0.a1 Prupe.3G080600_v2.0.a1 Prupe.3G080800_v2.0.a1 Prupe.3G083200_v2.0.a1 Prupe.3G099800_v2.0.a1 Prupe.3G099800_v2.0.a1
pyrus_communis pycom17g20860 pycom17g20910 pycom17g22790
rosa_chinensis RchiOBHm_Chr2g0136891 RchiOBHm_Chr2g0136901 RchiOBHm_Chr2g0167711 RchiOBHm_Chr2g0167721 RchiOBHm_Chr2g0167731 RchiOBHm_Chr4g0407611
rosa_laevigata RLG00000018943 RLG00000019278 RLG00000019282 RLG00000019587 RLG00000021748 RLG00000021749 RLG00000031057
rosa_multiflora Rmu_sc0002143.1_g000003 Rmu_sc0002782.1_g000001 Rmu_sc0023879.1_g000001 Rmu_sc0042171.1_g000001
rosa_roxburghii Rroxscaffold_2G00083300 Rroxscaffold_2G00107890 Rroxscaffold_2G00116680
rosa_rugosa Rorug02G0271700 Rorug02G0334900 Rorug02G0531800 Rorug02G0531800
rosa_samantha Rh2AG359900 Rh2AG385100 Rh2AG600500 Rh2AG600600 Rh2BG392200 Rh2BG611000 Rh2BG611100 Rh2BG611200 Rh2CG343100 Rh2CG343300 Rh2CG372300 Rh2CG581400 Rh2CG581500 Rh2DG382900 Rh2DG623700 Rh5AG027500 Rh5CG029900
rosa_wichuraiana Rw2G031490 Rw2G049850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1284
AarI CACCTGC 2 cut(s) 1085, 1481
AatII GACGTC 1 cut(s) 2481
Acc36I ACCTGC 2 cut(s) 1085, 1481
AccB1I GGYRCC 1 cut(s) 1792
AccB7I CCANNNNNTGG 1 cut(s) 727
AccI GTMKAC 1 cut(s) 1681
AccII CGCG 2 cut(s) 516, 1052
AccIII TCCGGA 2 cut(s) 436, 1246
AciI CCGC 3 cut(s) 279, 428, 1351
AclWI GGATC 2 cut(s) 32, 824
AcoI YGGCCR 2 cut(s) 2148, 2533
AcsI RAATTY 8 cut(s) 687, 764, 943, 999, 1142, 1578, 2386, 2494
AcuI CTGAAG 3 cut(s) 218, 1527, 2486
AcyI GRCGYC 1 cut(s) 2478
AfaI GTAC 4 cut(s) 593, 825, 1363, 2364
AfiI CCNNNNNNNGG 3 cut(s) 467, 634, 727
AflII CTTAAG 1 cut(s) 1178
AflIII ACRYGT 2 cut(s) 87, 588
AjiI CACGTC 2 cut(s) 160, 333
AjnI CCWGG 3 cut(s) 641, 1341, 2085
Alw21I GWGCWC 5 cut(s) 1336, 1942, 2286, 2359, 2632
Alw26I GTCTC 4 cut(s) 107, 643, 2092, 2247
AlwI GGATC 2 cut(s) 32, 824
Aor13HI TCCGGA 2 cut(s) 436, 1246
AoxI GGCC 5 cut(s) 144, 449, 1071, 2148, 2533
ApeKI GCWGC 3 cut(s) 12, 1978, 2067
ApoI RAATTY 8 cut(s) 687, 764, 943, 999, 1142, 1578, 2386, 2494
Asp700I GAANNNNTTC 5 cut(s) 663, 947, 1113, 1166, 1533
AspLEI GCGC 3 cut(s) 516, 1054, 1953
AspS9I GGNCC 2 cut(s) 1071, 2615
AsuC2I CCSGG 2 cut(s) 629, 2532
AsuHPI GGTGA 6 cut(s) 293, 925, 1109, 1307, 2002, 2038
AsuNHI GCTAGC 2 cut(s) 275, 1202
AvaII GGWCC 1 cut(s) 2615
BaeI ACNNNNGTAYC 2 cut(s) 1353, 1386
BalI TGGCCA 1 cut(s) 2150
BanI GGYRCC 1 cut(s) 1792
BanII GRGCYC 3 cut(s) 462, 1336, 2286
BarI GAAGNNNNNNTAC 2 cut(s) 807, 839
BauI CACGAG 2 cut(s) 867, 2305
BbsI GAAGAC 6 cut(s) 108, 666, 981, 1094, 1159, 2568
Bbv12I GWGCWC 5 cut(s) 1336, 1942, 2286, 2359, 2632
BbvI GCAGC 2 cut(s) 1990, 2054
BccI CCATC 5 cut(s) 37, 1179, 1285, 1555, 1735
BceAI ACGGC 1 cut(s) 2520
BciT130I CCWGG 3 cut(s) 643, 1343, 2087
BclI TGATCA 2 cut(s) 616, 1056
BcnI CCSGG 2 cut(s) 629, 2532
BcoDI GTCTC 4 cut(s) 107, 643, 2092, 2247
BfaI CTAG 4 cut(s) 276, 1203, 1337, 2105
BfrI CTTAAG 1 cut(s) 1178
BfuAI ACCTGC 2 cut(s) 1085, 1481
BglII AGATCT 1 cut(s) 579
BisI GCNGC 4 cut(s) 13, 429, 1979, 2068
BlsI GCNGC 4 cut(s) 14, 430, 1980, 2069
Bme1390I CCNGG 5 cut(s) 629, 643, 1343, 2087, 2532
Bme18I GGWCC 1 cut(s) 2615
BmgBI CACGTC 2 cut(s) 160, 333
BmgT120I GGNCC 2 cut(s) 1071, 2615
BmiI GGNNCC 4 cut(s) 500, 647, 1794, 2617
BmrFI CCNGG 5 cut(s) 629, 643, 1343, 2087, 2532
BmsI GCATC 4 cut(s) 89, 373, 415, 1362
BmtI GCTAGC 2 cut(s) 279, 1206
BpiI GAAGAC 6 cut(s) 108, 666, 981, 1094, 1159, 2568
BplI GAGNNNNNCTC 2 cut(s) 2056, 2088
BpmI CTGGAG 3 cut(s) 625, 1350, 2574
Bpu10I CCTNAGC 1 cut(s) 2358
BpuEI CTTGAG 3 cut(s) 304, 1638, 2270
BpuMI CCSGG 2 cut(s) 629, 2532
BsaAI YACGTR 1 cut(s) 591
BsaBI GATNNNNATC 1 cut(s) 615
BsaHI GRCGYC 1 cut(s) 2478
BsaI GGTCTC 2 cut(s) 643, 2247
BsaJI CCNNGG 2 cut(s) 1342, 2085
BsaWI WCCGGW 3 cut(s) 436, 994, 1246
BsaXI ACNNNNNCTCC 2 cut(s) 1389, 1419
Bsc4I CCNNNNNNNGG 3 cut(s) 467, 634, 727
Bse1I ACTGG 3 cut(s) 1848, 2557, 2570
Bse3DI GCAATG 1 cut(s) 1960
Bse8I GATNNNNATC 1 cut(s) 615
BseAI TCCGGA 2 cut(s) 436, 1246
BseBI CCWGG 3 cut(s) 643, 1343, 2087
BseDI CCNNGG 2 cut(s) 1342, 2085
BseGI GGATG 7 cut(s) 129, 231, 317, 787, 1296, 1315, 2328
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 3 cut(s) 467, 634, 727
BseMI GCAATG 1 cut(s) 1960
BseMII CTCAG 6 cut(s) 447, 1690, 1905, 2040, 2054, 2372
BseNI ACTGG 3 cut(s) 1848, 2557, 2570
BseRI GAGGAG 1 cut(s) 890
BseX3I CGGCCG 1 cut(s) 2533
BseXI GCAGC 2 cut(s) 1990, 2054
BseYI CCCAGC 1 cut(s) 416
BsgI GTGCAG 3 cut(s) 49, 182, 2053
Bsh1236I CGCG 2 cut(s) 516, 1052
Bsh1285I CGRYCG 1 cut(s) 2536
BshFI GGCC 5 cut(s) 146, 451, 1073, 2150, 2535
BshNI GGYRCC 1 cut(s) 1792
BsiEI CGRYCG 1 cut(s) 2536
BsiHKAI GWGCWC 5 cut(s) 1336, 1942, 2286, 2359, 2632
BsiSI CCGG 6 cut(s) 437, 628, 995, 1038, 1247, 2532
BslFI GGGAC 2 cut(s) 4, 2601
BslI CCNNNNNNNGG 3 cut(s) 467, 634, 727
BsmAI GTCTC 4 cut(s) 107, 643, 2092, 2247
BsmFI GGGAC 2 cut(s) 4, 2601
BsmI GAATGC 1 cut(s) 332
BsnI GGCC 5 cut(s) 146, 451, 1073, 2150, 2535
Bso31I GGTCTC 2 cut(s) 643, 2247
Bsp1286I GDGCHC 6 cut(s) 462, 1336, 1942, 2286, 2359, 2632
Bsp13I TCCGGA 2 cut(s) 436, 1246
Bsp143I GATC 8 cut(s) 37, 579, 616, 743, 802, 829, 1056, 1765
BspACI CCGC 3 cut(s) 279, 428, 1351
BspANI GGCC 5 cut(s) 146, 451, 1073, 2150, 2535
BspCNI CTCAG 6 cut(s) 448, 1689, 1904, 2039, 2055, 2371
BspEI TCCGGA 2 cut(s) 436, 1246
BspFNI CGCG 2 cut(s) 516, 1052
BspLI GGNNCC 4 cut(s) 500, 647, 1794, 2617
BspMI ACCTGC 2 cut(s) 1085, 1481
BspOI GCTAGC 2 cut(s) 279, 1206
BspPI GGATC 2 cut(s) 32, 824
BspQI GCTCTTC 1 cut(s) 2420
BspT107I GGYRCC 1 cut(s) 1792
BspTI CTTAAG 1 cut(s) 1178
BspTNI GGTCTC 2 cut(s) 643, 2247
BsrDI GCAATG 1 cut(s) 1960
BsrI ACTGG 3 cut(s) 1848, 2557, 2570
BssECI CCNNGG 2 cut(s) 1342, 2085
BssMI GATC 8 cut(s) 37, 579, 616, 743, 802, 829, 1056, 1765
BssNI GRCGYC 1 cut(s) 2478
BssSI CACGAG 2 cut(s) 867, 2305
Bst2BI CACGAG 2 cut(s) 867, 2305
Bst2UI CCWGG 3 cut(s) 643, 1343, 2087
Bst4CI ACNGT 5 cut(s) 70, 466, 2093, 2489, 2525
Bst6I CTCTTC 5 cut(s) 234, 573, 2056, 2420, 2462
BstACI GRCGYC 1 cut(s) 2478
BstAFI CTTAAG 1 cut(s) 1178
BstBAI YACGTR 1 cut(s) 591
BstC8I GCNNGC 4 cut(s) 277, 405, 473, 1204
BstEII GGTNACC 1 cut(s) 1346
BstF5I GGATG 7 cut(s) 129, 231, 317, 787, 1296, 1315, 2328
BstFNI CGCG 2 cut(s) 516, 1052
BstHHI GCGC 3 cut(s) 516, 1054, 1953
BstKTI GATC 8 cut(s) 40, 582, 619, 746, 805, 832, 1059, 1768
BstMAI GTCTC 4 cut(s) 107, 643, 2092, 2247
BstMBI GATC 8 cut(s) 37, 579, 616, 743, 802, 829, 1056, 1765
BstMCI CGRYCG 1 cut(s) 2536
BstMWI GCNNNNNNNGC 8 cut(s) 336, 413, 457, 511, 1051, 1325, 1987, 2202
BstNI CCWGG 3 cut(s) 643, 1343, 2087
BstNSI RCATGY 3 cut(s) 91, 475, 1380
BstPI GGTNACC 1 cut(s) 1346
BstSCI CCNGG 5 cut(s) 627, 641, 1341, 2085, 2530
BstUI CGCG 2 cut(s) 516, 1052
BstV1I GCAGC 2 cut(s) 1990, 2054
BstV2I GAAGAC 6 cut(s) 108, 666, 981, 1094, 1159, 2568
BstX2I RGATCY 1 cut(s) 579
BstXI CCANNNNNNTGG 1 cut(s) 896
BstYI RGATCY 1 cut(s) 579
BstZI CGGCCG 1 cut(s) 2533
BsuRI GGCC 5 cut(s) 146, 451, 1073, 2150, 2535
BtrI CACGTC 2 cut(s) 160, 333
BtsCI GGATG 7 cut(s) 129, 231, 317, 787, 1296, 1315, 2328
BtsIMutI CAGTG 3 cut(s) 462, 2530, 2550
BveI ACCTGC 2 cut(s) 1085, 1481
Cac8I GCNNGC 4 cut(s) 277, 405, 473, 1204
CfoI GCGC 3 cut(s) 516, 1054, 1953
Cfr13I GGNCC 2 cut(s) 1071, 2615
CseI GACGC 2 cut(s) 944, 2123
Csp6I GTAC 4 cut(s) 592, 824, 1362, 2363
CviQI GTAC 4 cut(s) 592, 824, 1362, 2363
DpnI GATC 8 cut(s) 39, 581, 618, 745, 804, 831, 1058, 1767
DpnII GATC 8 cut(s) 37, 579, 616, 743, 802, 829, 1056, 1765
EaeI YGGCCR 2 cut(s) 2148, 2533
EagI CGGCCG 1 cut(s) 2533
Eam1104I CTCTTC 5 cut(s) 234, 573, 2056, 2420, 2462
EarI CTCTTC 5 cut(s) 234, 573, 2056, 2420, 2462
Ecl136II GAGCTC 2 cut(s) 1334, 2284
EclXI CGGCCG 1 cut(s) 2533
Eco147I AGGCCT 1 cut(s) 146
Eco24I GRGCYC 3 cut(s) 462, 1336, 2286
Eco31I GGTCTC 2 cut(s) 643, 2247
Eco32I GATATC 1 cut(s) 676
Eco47I GGWCC 1 cut(s) 2615
Eco52I CGGCCG 1 cut(s) 2533
Eco53kI GAGCTC 2 cut(s) 1334, 2284
Eco57I CTGAAG 3 cut(s) 218, 1527, 2486
Eco91I GGTNACC 1 cut(s) 1346
EcoICRI GAGCTC 2 cut(s) 1334, 2284
EcoO65I GGTNACC 1 cut(s) 1346
EcoRII CCWGG 3 cut(s) 641, 1341, 2085
EcoRV GATATC 1 cut(s) 676
EcoT22I ATGCAT 2 cut(s) 1378, 1416
EcoT38I GRGCYC 3 cut(s) 462, 1336, 2286
FalI AAGNNNNNCTT 4 cut(s) 944, 976, 1460, 1492
FaqI GGGAC 2 cut(s) 4, 2601
FauNDI CATATG 1 cut(s) 2432
FbaI TGATCA 2 cut(s) 616, 1056
FblI GTMKAC 1 cut(s) 1681
Fnu4HI GCNGC 4 cut(s) 13, 429, 1979, 2068
FokI GGATG 7 cut(s) 116, 238, 324, 794, 1303, 1322, 2315
FriOI GRGCYC 3 cut(s) 462, 1336, 2286
Fsp4HI GCNGC 4 cut(s) 13, 429, 1979, 2068
FspBI CTAG 4 cut(s) 276, 1203, 1337, 2105
GlaI GCGC 3 cut(s) 515, 1053, 1952
GluI GCNGC 4 cut(s) 13, 429, 1979, 2068
GsaI CCCAGC 1 cut(s) 420
GsuI CTGGAG 3 cut(s) 625, 1350, 2574
HaeIII GGCC 5 cut(s) 146, 451, 1073, 2150, 2535
HapII CCGG 6 cut(s) 437, 628, 995, 1038, 1247, 2532
HgaI GACGC 2 cut(s) 944, 2123
HhaI GCGC 3 cut(s) 516, 1054, 1953
Hin1I GRCGYC 1 cut(s) 2478
Hin6I GCGC 3 cut(s) 514, 1052, 1951
HinP1I GCGC 3 cut(s) 514, 1052, 1951
HincII GTYRAC 2 cut(s) 1853, 2004
HindII GTYRAC 2 cut(s) 1853, 2004
HindIII AAGCTT 2 cut(s) 1885, 2607
HinfI GANTC 6 cut(s) 731, 1016, 1667, 1864, 2024, 2290
HpaII CCGG 6 cut(s) 437, 628, 995, 1038, 1247, 2532
HphI GGTGA 6 cut(s) 293, 925, 1109, 1307, 2002, 2038
Hpy166II GTNNAC 4 cut(s) 718, 1682, 1853, 2004
Hpy188I TCNGA 9 cut(s) 134, 680, 807, 1774, 1894, 2037, 2121, 2192, 2314
Hpy8I GTNNAC 4 cut(s) 718, 1682, 1853, 2004
HpyAV CCTTC 5 cut(s) 148, 185, 1142, 1605, 2225
HpyCH4III ACNGT 5 cut(s) 70, 466, 2093, 2489, 2525
HpyCH4IV ACGT 6 cut(s) 108, 159, 332, 590, 1616, 2478
HpyF10VI GCNNNNNNNGC 8 cut(s) 336, 413, 457, 511, 1051, 1325, 1987, 2202
HpySE526I ACGT 6 cut(s) 108, 159, 332, 590, 1616, 2478
Hsp92I GRCGYC 1 cut(s) 2478
HspAI GCGC 3 cut(s) 514, 1052, 1951
Kpn2I TCCGGA 2 cut(s) 436, 1246
Ksp22I TGATCA 2 cut(s) 616, 1056
Kzo9I GATC 8 cut(s) 37, 579, 616, 743, 802, 829, 1056, 1765
LguI GCTCTTC 1 cut(s) 2420
LmnI GCTCC 4 cut(s) 1331, 1607, 1840, 2511
Lsp1109I GCAGC 2 cut(s) 1990, 2054
LweI GCATC 4 cut(s) 89, 373, 415, 1362
MaeI CTAG 4 cut(s) 276, 1203, 1337, 2105
MaeII ACGT 6 cut(s) 108, 159, 332, 590, 1616, 2478
MaeIII GTNAC 7 cut(s) 5, 125, 1346, 1451, 2021, 2044, 2058
MalI GATC 8 cut(s) 39, 581, 618, 745, 804, 831, 1058, 1767
MboI GATC 8 cut(s) 37, 579, 616, 743, 802, 829, 1056, 1765
MfeI CAATTG 2 cut(s) 1515, 1797
MflI RGATCY 1 cut(s) 579
MhlI GDGCHC 6 cut(s) 462, 1336, 1942, 2286, 2359, 2632
MlsI TGGCCA 1 cut(s) 2150
MluNI TGGCCA 1 cut(s) 2150
MlyI GAGTC 1 cut(s) 2018
MmeI TCCRAC 5 cut(s) 52, 157, 799, 1287, 2356
Mox20I TGGCCA 1 cut(s) 2150
Mph1103I ATGCAT 2 cut(s) 1378, 1416
MroI TCCGGA 2 cut(s) 436, 1246
MroXI GAANNNNTTC 5 cut(s) 663, 947, 1113, 1166, 1533
MscI TGGCCA 1 cut(s) 2150
MseI TTAA 7 cut(s) 371, 389, 1124, 1179, 1371, 1754, 2579
MslI CAYNNNNRTG 2 cut(s) 88, 1269
Msp20I TGGCCA 1 cut(s) 2150
MspA1I CMGCKG 1 cut(s) 407
MspCI CTTAAG 1 cut(s) 1178
MspI CCGG 6 cut(s) 437, 628, 995, 1038, 1247, 2532
MspR9I CCNGG 5 cut(s) 629, 643, 1343, 2087, 2532
MunI CAATTG 2 cut(s) 1515, 1797
Mva1269I GAATGC 1 cut(s) 332
MvaI CCWGG 3 cut(s) 643, 1343, 2087
MvnI CGCG 2 cut(s) 516, 1052
MwoI GCNNNNNNNGC 8 cut(s) 336, 413, 457, 511, 1051, 1325, 1987, 2202
NciI CCSGG 2 cut(s) 629, 2532
NdeI CATATG 1 cut(s) 2432
NdeII GATC 8 cut(s) 37, 579, 616, 743, 802, 829, 1056, 1765
NheI GCTAGC 2 cut(s) 275, 1202
NlaIV GGNNCC 4 cut(s) 500, 647, 1794, 2617
NmuCI GTSAC 4 cut(s) 125, 1451, 2021, 2044
NsiI ATGCAT 2 cut(s) 1378, 1416
NspI RCATGY 3 cut(s) 91, 475, 1380
PaeI GCATGC 1 cut(s) 475
PaqCI CACCTGC 2 cut(s) 1085, 1481
PceI AGGCCT 1 cut(s) 146
PciI ACATGT 1 cut(s) 87
PciSI GCTCTTC 1 cut(s) 2420
PctI GAATGC 1 cut(s) 332
PdmI GAANNNNTTC 5 cut(s) 663, 947, 1113, 1166, 1533
PfeI GAWTC 5 cut(s) 731, 1016, 1667, 1864, 2290
PflMI CCANNNNNTGG 1 cut(s) 727
PfoI TCCNGGA 1 cut(s) 641
PkrI GCNGC 4 cut(s) 14, 430, 1980, 2069
PleI GAGTC 1 cut(s) 2018
PpsI GAGTC 1 cut(s) 2018
Ppu21I YACGTR 1 cut(s) 591
PscI ACATGT 1 cut(s) 87
PsiI TTATAA 1 cut(s) 1284
Psp124BI GAGCTC 2 cut(s) 1336, 2286
Psp6I CCWGG 3 cut(s) 641, 1341, 2085
PspEI GGTNACC 1 cut(s) 1346
PspFI CCCAGC 1 cut(s) 416
PspGI CCWGG 3 cut(s) 641, 1341, 2085
PspN4I GGNNCC 4 cut(s) 500, 647, 1794, 2617
PspPI GGNCC 2 cut(s) 1071, 2615
PsuI RGATCY 1 cut(s) 579
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 4 cut(s) 593, 825, 1363, 2364
RsaNI GTAC 4 cut(s) 592, 824, 1362, 2363
RseI CAYNNNNRTG 2 cut(s) 88, 1269
SacI GAGCTC 2 cut(s) 1336, 2286
SapI GCTCTTC 1 cut(s) 2420
SaqAI TTAA 7 cut(s) 371, 389, 1124, 1179, 1371, 1754, 2579
SatI GCNGC 4 cut(s) 13, 429, 1979, 2068
Sau3AI GATC 8 cut(s) 37, 579, 616, 743, 802, 829, 1056, 1765
Sau96I GGNCC 2 cut(s) 1071, 2615
SchI GAGTC 1 cut(s) 2018
ScrFI CCNGG 5 cut(s) 629, 643, 1343, 2087, 2532
SduI GDGCHC 6 cut(s) 462, 1336, 1942, 2286, 2359, 2632
SfaNI GCATC 4 cut(s) 89, 373, 415, 1362
SinI GGWCC 1 cut(s) 2615
SmiMI CAYNNNNRTG 2 cut(s) 88, 1269
SmlI CTYRAG 4 cut(s) 319, 1178, 1653, 2285
SmoI CTYRAG 4 cut(s) 319, 1178, 1653, 2285
SphI GCATGC 1 cut(s) 475
SseBI AGGCCT 1 cut(s) 146
SsiI CCGC 3 cut(s) 279, 428, 1351
SspMI CTAG 4 cut(s) 276, 1203, 1337, 2105
SstI GAGCTC 2 cut(s) 1336, 2286
StuI AGGCCT 1 cut(s) 146
StyD4I CCNGG 5 cut(s) 627, 641, 1341, 2085, 2530
TaaI ACNGT 5 cut(s) 70, 466, 2093, 2489, 2525
TaiI ACGT 6 cut(s) 111, 162, 335, 593, 1619, 2481
TaqI TCGA 3 cut(s) 52, 381, 742
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 5 cut(s) 731, 1016, 1667, 1864, 2290
Tru1I TTAA 7 cut(s) 371, 389, 1124, 1179, 1371, 1754, 2579
Tru9I TTAA 7 cut(s) 371, 389, 1124, 1179, 1371, 1754, 2579
TscAI CASTG 3 cut(s) 469, 2530, 2557
TseFI GTSAC 4 cut(s) 125, 1451, 2021, 2044
TseI GCWGC 3 cut(s) 12, 1978, 2067
Tsp45I GTSAC 4 cut(s) 125, 1451, 2021, 2044
TspRI CASTG 3 cut(s) 469, 2530, 2557
Van91I CCANNNNNTGG 1 cut(s) 727
Vha464I CTTAAG 1 cut(s) 1178
VpaK11BI GGWCC 1 cut(s) 2615
XapI RAATTY 8 cut(s) 687, 764, 943, 999, 1142, 1578, 2386, 2494
XbaI TCTAGA 2 cut(s) 1336, 2104
XceI RCATGY 3 cut(s) 91, 475, 1380
XcmI CCANNNNNNNNNTGG 1 cut(s) 2204
XmiI GTMKAC 1 cut(s) 1681
XmnI GAANNNNTTC 5 cut(s) 663, 947, 1113, 1166, 1533
XspI CTAG 4 cut(s) 276, 1203, 1337, 2105
ZraI GACGTC 1 cut(s) 2479
Zsp2I ATGCAT 2 cut(s) 1378, 1416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.