RLG00000031057

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000363
Physical Location & Seq
Forward (+)
1644199 .. 1645631
1433 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031057

Sequence Viewer

Length: 921 bp
ATGGAAGCTATGATTGATGATGTTTGGACCTACATTGAAGATGCAAGTGAAAGTCATCGGCGTGTATGGGATGGGAGGGGTGGGCAAAACCACCCTCCTTACCAACATCTTAATAGCTCCAAACACATTCTTCTTCCTTTCAATTATGTTCTATGGGTCACTGTCTCCAGGGAGTTGAAGCTGGACAACATACAAGATAAAATTGCGCGAGATGTTGGACTTATGAGTGACAAGTGGAAGGATCAATCTCAACAAGAAAAGGCTCGAGACATCTTCAGTCTACTACACAATAAAATAATTTTGTTATTGTTGGATGACATTTGGGATCAAATCGACTTGTTCAAATTAGGCATTTCAATTTTTGATCGCTCCAAGTTACTCTTCTCAACTCGGTCCGAAGATGTCTGTGGACGTATGGCAGTTCAGAAAAAGATTAAAGTTCAGTGCTTGGAGTGGTCTGAAGCTCTGGACTTGTTCCAACAGACAGTTGGAAATGAAATACTTGTCATTCACCCTGAAATCCCAAGAGAGTTTTCTAGTGCAAACAGGTGCTCAGTTAGTGAAAATCTAGCTGAAACACCCACATGTCCTAATCTGCTAATGTTGTTTCTTCGTGGTAATGAGTTGAGATGGATTGGCAAAGAATTTTTAGACTTCATGCCTGTTTTGGTAGTGTTGGATTTGAGTGAAAACATTTCTCTAAAAAGGCTGCCTTCTAGAGTTTCGAAGCTTGTTGCACTACAGCAGCTCAATTTGTCCAAAAATCATTTACAAGAGTTGTCAGATGAATTAAAATTGTTAGTAAAGCTACTTGTTTTGAACTTGGAGGACACACATAACCTCAAGTCTGTTCCACCAAATATGATGTCAAGTCTTCTGAGGTTAAGAATTTTAAGAATGGCACGTCCTCAGAAGACTTGA

Protein Analysis

307

Amino Acids

35.49

Weight (kDa)

6.72

Isoelectric Point (pI)

53.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 38 - 165 1.9e-26 NB-ARC domain
LRR_8 PF13855 197 - 257 3.1e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12210 AT1G12210 AT1G12210 AT1G12220 AT1G12220 AT1G12220 AT1G12280 AT1G12280 AT1G12290 AT1G12290 AT1G12290 AT1G12290 AT1G15890 AT1G15890 AT1G51480 AT1G52660 AT1G52660 AT1G61180 AT1G61180 AT1G61190 AT1G61190 AT1G61190 AT1G61190 AT1G61300 AT1G61300 AT1G61310 AT3G15700 AT3G15700 AT4G10780 AT4G10780 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43740 AT5G43740 AT5G63020
fragaria_vesca FvH4_6g30050 FvH4_6g48220
malus_domestica MD09G1055800.v1.1 MD09G1198800.v1.1 MD17G1204800.v1.1 MD17G1204900.v1.1 MD17G1205000.v1.1 MD17G1205300.v1.1 MD17G1205700.v1.1 MD17G1205800.v1.1 MD17G1224100.v1.1
prunus_persica Prupe.3G054800_v2.0.a1 Prupe.3G054800_v2.0.a1 Prupe.3G080300_v2.0.a1 Prupe.3G080400_v2.0.a1 Prupe.3G080500_v2.0.a1 Prupe.3G080600_v2.0.a1 Prupe.3G080800_v2.0.a1 Prupe.3G083200_v2.0.a1 Prupe.3G099800_v2.0.a1 Prupe.3G099800_v2.0.a1
pyrus_communis pycom17g20860 pycom17g20910 pycom17g22790
rosa_chinensis RchiOBHm_Chr2g0136891 RchiOBHm_Chr2g0136901 RchiOBHm_Chr2g0167711 RchiOBHm_Chr2g0167721 RchiOBHm_Chr2g0167731 RchiOBHm_Chr4g0407611
rosa_laevigata RLG00000018943 RLG00000019278 RLG00000019282 RLG00000019587 RLG00000021748 RLG00000021749 RLG00000031057
rosa_multiflora Rmu_sc0002143.1_g000003 Rmu_sc0002782.1_g000001 Rmu_sc0023879.1_g000001 Rmu_sc0042171.1_g000001
rosa_roxburghii Rroxscaffold_2G00083300 Rroxscaffold_2G00107890 Rroxscaffold_2G00116680
rosa_rugosa Rorug02G0271700 Rorug02G0334900 Rorug02G0531800 Rorug02G0531800
rosa_samantha Rh2AG359900 Rh2AG385100 Rh2AG600500 Rh2AG600600 Rh2BG392200 Rh2BG611000 Rh2BG611100 Rh2BG611200 Rh2CG343100 Rh2CG343300 Rh2CG372300 Rh2CG581400 Rh2CG581500 Rh2DG382900 Rh2DG623700 Rh5AG027500 Rh5CG029900
rosa_wichuraiana Rw2G031490 Rw2G049850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 280
AccII CGCG 1 cut(s) 208
AclWI GGATC 2 cut(s) 249, 333
AcsI RAATTY 2 cut(s) 644, 888
AcuI CTGAAG 2 cut(s) 259, 480
AflIII ACRYGT 1 cut(s) 584
AgsI TTSAA 6 cut(s) 38, 142, 178, 343, 357, 820
AjiI CACGTC 1 cut(s) 905
AjnI CCWGG 1 cut(s) 167
AjuI GAANNNNNNNTTGG 4 cut(s) 365, 397, 517, 549
AluBI AGCT 8 cut(s) 8, 117, 181, 464, 572, 730, 748, 808
AluI AGCT 8 cut(s) 8, 117, 181, 464, 572, 730, 748, 808
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 2 cut(s) 169, 261
AlwI GGATC 2 cut(s) 249, 333
Ama87I CYCGRG 1 cut(s) 264
ApeKI GCWGC 2 cut(s) 709, 745
ApoI RAATTY 2 cut(s) 644, 888
ArsI GACNNNNNNTTYG 2 cut(s) 851, 883
AspLEI GCGC 1 cut(s) 208
AspS9I GGNCC 2 cut(s) 27, 393
AsuHPI GGTGA 1 cut(s) 503
AsuII TTCGAA 1 cut(s) 725
AvaI CYCGRG 1 cut(s) 264
AvaII GGWCC 2 cut(s) 27, 393
BbsI GAAGAC 1 cut(s) 866
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 2 cut(s) 696, 757
BccI CCATC 2 cut(s) 65, 624
BciT130I CCWGG 1 cut(s) 169
BcoDI GTCTC 2 cut(s) 169, 261
BfaI CTAG 3 cut(s) 537, 569, 717
BfmI CTRYAG 1 cut(s) 740
BisI GCNGC 2 cut(s) 710, 746
BlsI GCNGC 2 cut(s) 711, 747
Bme1390I CCNGG 1 cut(s) 169
Bme18I GGWCC 2 cut(s) 27, 393
BmeT110I CYCGRG 1 cut(s) 264
BmgBI CACGTC 1 cut(s) 905
BmgT120I GGNCC 2 cut(s) 27, 393
BmrFI CCNGG 1 cut(s) 169
BmsI GCATC 1 cut(s) 31
BpiI GAAGAC 1 cut(s) 866
BpmI CTGGAG 1 cut(s) 151
Bpu14I TTCGAA 1 cut(s) 725
BpuEI CTTGAG 1 cut(s) 827
BsaJI CCNNGG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 169
BseDI CCNNGG 1 cut(s) 168
BseGI GGATG 2 cut(s) 76, 319
BseMII CTCAG 2 cut(s) 567, 869
BseXI GCAGC 2 cut(s) 696, 757
Bsh1236I CGCG 1 cut(s) 208
BsiHKAI GWGCWC 1 cut(s) 554
BsiHKCI CYCGRG 1 cut(s) 264
BsmAI GTCTC 2 cut(s) 169, 261
BsoBI CYCGRG 1 cut(s) 264
Bsp119I TTCGAA 1 cut(s) 725
Bsp1286I GDGCHC 1 cut(s) 554
Bsp143I GATC 3 cut(s) 241, 325, 364
BspCNI CTCAG 2 cut(s) 566, 870
BspFNI CGCG 1 cut(s) 208
BspPI GGATC 2 cut(s) 249, 333
BspT104I TTCGAA 1 cut(s) 725
BssECI CCNNGG 1 cut(s) 168
BssMI GATC 3 cut(s) 241, 325, 364
Bst2UI CCWGG 1 cut(s) 169
Bst4CI ACNGT 2 cut(s) 163, 487
Bst6I CTCTTC 1 cut(s) 386
BstBI TTCGAA 1 cut(s) 725
BstDEI CTNAG 3 cut(s) 553, 878, 909
BstF5I GGATG 2 cut(s) 76, 319
BstFNI CGCG 1 cut(s) 208
BstHHI GCGC 1 cut(s) 208
BstKTI GATC 3 cut(s) 244, 328, 367
BstMAI GTCTC 2 cut(s) 169, 261
BstMBI GATC 3 cut(s) 241, 325, 364
BstNI CCWGG 1 cut(s) 169
BstNSI RCATGY 1 cut(s) 588
BstSCI CCNGG 1 cut(s) 167
BstSFI CTRYAG 1 cut(s) 740
BstUI CGCG 1 cut(s) 208
BstV1I GCAGC 2 cut(s) 696, 757
BstV2I GAAGAC 1 cut(s) 866
BtrI CACGTC 1 cut(s) 905
BtsCI GGATG 2 cut(s) 76, 319
BtsIMutI CAGTG 2 cut(s) 159, 449
CfoI GCGC 1 cut(s) 208
Cfr13I GGNCC 2 cut(s) 27, 393
CpoI CGGWCCG 1 cut(s) 393
CspI CGGWCCG 1 cut(s) 393
CviAII CATG 2 cut(s) 585, 658
DdeI CTNAG 3 cut(s) 553, 878, 909
DpnI GATC 3 cut(s) 243, 327, 366
DpnII GATC 3 cut(s) 241, 325, 364
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
Eco47I GGWCC 2 cut(s) 27, 393
Eco57I CTGAAG 2 cut(s) 259, 480
Eco88I CYCGRG 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 167
FaeI CATG 2 cut(s) 588, 661
FalI AAGNNNNNCTT 4 cut(s) 365, 397, 697, 729
FatI CATG 2 cut(s) 584, 657
FblI GTMKAC 1 cut(s) 280
Fnu4HI GCNGC 2 cut(s) 710, 746
FokI GGATG 2 cut(s) 83, 326
Fsp4HI GCNGC 2 cut(s) 710, 746
FspBI CTAG 3 cut(s) 537, 569, 717
GlaI GCGC 1 cut(s) 207
GluI GCNGC 2 cut(s) 710, 746
GsuI CTGGAG 1 cut(s) 151
HhaI GCGC 1 cut(s) 208
Hin1II CATG 2 cut(s) 588, 661
Hin6I GCGC 1 cut(s) 206
HinP1I GCGC 1 cut(s) 206
HindIII AAGCTT 1 cut(s) 728
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 2 cut(s) 281, 410
Hpy188I TCNGA 6 cut(s) 397, 426, 460, 784, 879, 912
Hpy188III TCNNGA 3 cut(s) 266, 467, 717
Hpy8I GTNNAC 2 cut(s) 281, 410
HpyAV CCTTC 2 cut(s) 232, 723
HpyCH4III ACNGT 2 cut(s) 163, 487
HpyCH4IV ACGT 2 cut(s) 412, 904
HpyCH4V TGCA 3 cut(s) 44, 542, 737
HpyF3I CTNAG 3 cut(s) 553, 878, 909
HpySE526I ACGT 2 cut(s) 412, 904
Hsp92II CATG 2 cut(s) 588, 661
HspAI GCGC 1 cut(s) 206
Kzo9I GATC 3 cut(s) 241, 325, 364
LmnI GCTCC 2 cut(s) 122, 374
LpnPI CCDG 7 cut(s) 154, 167, 181, 452, 528, 532, 675
Lsp1109I GCAGC 2 cut(s) 696, 757
LweI GCATC 1 cut(s) 31
MaeI CTAG 3 cut(s) 537, 569, 717
MaeII ACGT 2 cut(s) 412, 904
MaeIII GTNAC 3 cut(s) 157, 227, 375
MalI GATC 3 cut(s) 243, 327, 366
MboI GATC 3 cut(s) 241, 325, 364
MboII GAAGA 8 cut(s) 50, 122, 125, 265, 373, 410, 602, 866
MhlI GDGCHC 1 cut(s) 554
MmeI TCCRAC 5 cut(s) 196, 291, 469, 502, 657
MnlI CCTC 6 cut(s) 69, 105, 820, 851, 873, 918
MseI TTAA 5 cut(s) 111, 435, 791, 884, 893
MslI CAYNNNNRTG 2 cut(s) 60, 583
MspR9I CCNGG 1 cut(s) 169
MvaI CCWGG 1 cut(s) 169
MvnI CGCG 1 cut(s) 208
NdeII GATC 3 cut(s) 241, 325, 364
NlaIII CATG 2 cut(s) 588, 661
NmuCI GTSAC 2 cut(s) 157, 227
NspI RCATGY 1 cut(s) 588
NspV TTCGAA 1 cut(s) 725
PaeR7I CTCGAG 1 cut(s) 264
PciI ACATGT 1 cut(s) 584
PkrI GCNGC 2 cut(s) 711, 747
PscI ACATGT 1 cut(s) 584
Psp6I CCWGG 1 cut(s) 167
PspGI CCWGG 1 cut(s) 167
PspPI GGNCC 2 cut(s) 27, 393
RseI CAYNNNNRTG 2 cut(s) 60, 583
Rsr2I CGGWCCG 1 cut(s) 393
RsrII CGGWCCG 1 cut(s) 393
SaqAI TTAA 5 cut(s) 111, 435, 791, 884, 893
SatI GCNGC 2 cut(s) 710, 746
Sau3AI GATC 3 cut(s) 241, 325, 364
Sau96I GGNCC 2 cut(s) 27, 393
ScrFI CCNGG 1 cut(s) 169
SduI GDGCHC 1 cut(s) 554
SfaNI GCATC 1 cut(s) 31
SfcI CTRYAG 1 cut(s) 740
Sfr274I CTCGAG 1 cut(s) 264
SfuI TTCGAA 1 cut(s) 725
SinI GGWCC 2 cut(s) 27, 393
SlaI CTCGAG 1 cut(s) 264
SmiMI CAYNNNNRTG 2 cut(s) 60, 583
SmlI CTYRAG 2 cut(s) 264, 842
SmoI CTYRAG 2 cut(s) 264, 842
SspMI CTAG 3 cut(s) 537, 569, 717
StyD4I CCNGG 1 cut(s) 167
TaaI ACNGT 2 cut(s) 163, 487
TaiI ACGT 2 cut(s) 415, 907
TaqI TCGA 3 cut(s) 265, 333, 725
TaqII GACCGA 1 cut(s) 381
Tru1I TTAA 5 cut(s) 111, 435, 791, 884, 893
Tru9I TTAA 5 cut(s) 111, 435, 791, 884, 893
TscAI CASTG 2 cut(s) 166, 449
TseFI GTSAC 2 cut(s) 157, 227
TseI GCWGC 2 cut(s) 709, 745
Tsp45I GTSAC 2 cut(s) 157, 227
TspDTI ATGAA 3 cut(s) 510, 646, 801
TspRI CASTG 2 cut(s) 166, 449
VpaK11BI GGWCC 2 cut(s) 27, 393
XapI RAATTY 2 cut(s) 644, 888
XbaI TCTAGA 1 cut(s) 716
XceI RCATGY 1 cut(s) 588
XcmI CCANNNNNNNNNTGG 1 cut(s) 485
XhoI CTCGAG 1 cut(s) 264
XmiI GTMKAC 1 cut(s) 280
XspI CTAG 3 cut(s) 537, 569, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.