Rh5AG027500

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000363
Physical Location & Seq
Reverse (-)
1900140 .. 1901447
1308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG027500.1

Sequence Viewer

Length: 1308 bp
ATGGAAGCTATGATTGATGAGGTTTGGACCTACATTGAAGATGCAAATGTGAAAGTCATTGGCGTATATGGGATGGGAGGGGTGGGCAAAACCACCCTCCTTACCAACATCTTTAACAGCTCCAAACACATTCTTCTTCCTTTCAATTATGTTCTATGGGTCACTGTCTCCAGGGAGTTGAAGCTGGACAACATACAAGATAAGATTGCGCCAGATGTTGGACTTATGAGTGACAAGTGGGAGGATCAATCTCAACAAGAAAAGGCTCGAGACATCTTCAGTCTACTACACAATAAAAGAATTTTGTTATTGTTGGATGACATTTGGGATCGAATCGACTTGTTCAAATTAGGCATTCCAATTTCTGATCGCTCCAAGTTACTCTTCTCAACTCGGTCCGAAGATGTCTGTGGACGTATGGCAGTTCAGAAAAAGATTAAAGTTCAGTGCTTGGAGTGGTCTGAAGCTTTGGACTTGTTCCAACAGACAGTTGGAAATGAAGTACTTGCCATTCAACCTGAAATCCCAAGGCTTGCTGAAACTGTAGTGAAAGAGTGTGGGGGTCTTCCACTTGTATTAAAAACAGTCGGTCGGGCCATGTCTTCCAAGAAGACTCCGCAAGAATGGAAACATGCGATTACAATCTTACAGAAAGTTGCCTCAGAGTTCTCAGAAATGGGAGAGGAGGTGTTTCCTATCCTAAAATATAGTTATGACAGTTTACCGAGCGACAAGATCAAAGCATGCTTCTTATACTGTGCCTTATTTCCAGAAGATTATGGCATAGCCATAGATGAGTTATTGGATTGTTGGATGTGTGAGGAACTATTGAAATATTCTAATGTGGAAGAAGCACGAAACGAGACCTATTATATTATAGGTATTCTCCTAAATGCTTGTTTGTTGGAAGAGGTGGACGTTGGAGTGAAAATTCATGATGTAATTCGTGATATGGCATTGTGGTTAGCTTCTGATCCTTCACAGAGTTTTCTAGTGCAAACAGGTGCTCAGTTAGTTAAAGTACCTATCTTGGGAAAATGGGAAAAATCGAAAGGGGTTTCATTTTCTGTTAATTGCCTTAAAGATCTAGCTGAAACACCCACATGTCCCAATCTGCTAATGTTGTTTCTTCGTGGTAATGAGTTGAGATGGATTGGCAAAGAATTTTTTGACTTCATGCCTGTTTTGGTAGTGTTGGATTTGAGTGAAAACATTTCTCTAAAAAAACTGCCTTCTGGAGTTTCGAAACTTGTTGCACTACAACAGCTCAATTTATTCAAAACTCATTTTGCAAGAGTTGCCAGATGA

Protein Analysis

435

Amino Acids

49.36

Weight (kDa)

5.26

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 2 - 166 9e-44 NB-ARC domain
WHD_DRP PF23559 255 - 319 4.7e-06 Disease resistance protein Winged helix domain
LRR_8 PF13855 370 - 429 2.5e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12210 AT1G12210 AT1G12210 AT1G12220 AT1G12220 AT1G12220 AT1G12280 AT1G12280 AT1G12290 AT1G12290 AT1G12290 AT1G12290 AT1G15890 AT1G15890 AT1G51480 AT1G52660 AT1G52660 AT1G61180 AT1G61180 AT1G61190 AT1G61190 AT1G61190 AT1G61190 AT1G61300 AT1G61300 AT1G61310 AT3G15700 AT3G15700 AT4G10780 AT4G10780 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43740 AT5G43740 AT5G63020
fragaria_vesca FvH4_6g30050 FvH4_6g48220
malus_domestica MD09G1055800.v1.1 MD09G1198800.v1.1 MD17G1204800.v1.1 MD17G1204900.v1.1 MD17G1205000.v1.1 MD17G1205300.v1.1 MD17G1205700.v1.1 MD17G1205800.v1.1 MD17G1224100.v1.1
prunus_persica Prupe.3G054800_v2.0.a1 Prupe.3G054800_v2.0.a1 Prupe.3G080300_v2.0.a1 Prupe.3G080400_v2.0.a1 Prupe.3G080500_v2.0.a1 Prupe.3G080600_v2.0.a1 Prupe.3G080800_v2.0.a1 Prupe.3G083200_v2.0.a1 Prupe.3G099800_v2.0.a1 Prupe.3G099800_v2.0.a1
pyrus_communis pycom17g20860 pycom17g20910 pycom17g22790
rosa_chinensis RchiOBHm_Chr2g0136891 RchiOBHm_Chr2g0136901 RchiOBHm_Chr2g0167711 RchiOBHm_Chr2g0167721 RchiOBHm_Chr2g0167731 RchiOBHm_Chr4g0407611
rosa_laevigata RLG00000018943 RLG00000019278 RLG00000019282 RLG00000019587 RLG00000021748 RLG00000021749 RLG00000031057
rosa_multiflora Rmu_sc0002143.1_g000003 Rmu_sc0002782.1_g000001 Rmu_sc0023879.1_g000001 Rmu_sc0042171.1_g000001
rosa_roxburghii Rroxscaffold_2G00083300 Rroxscaffold_2G00107890 Rroxscaffold_2G00116680
rosa_rugosa Rorug02G0271700 Rorug02G0334900 Rorug02G0531800 Rorug02G0531800
rosa_samantha Rh2AG359900 Rh2AG385100 Rh2AG600500 Rh2AG600600 Rh2BG392200 Rh2BG611000 Rh2BG611100 Rh2BG611200 Rh2CG343100 Rh2CG343300 Rh2CG372300 Rh2CG581400 Rh2CG581500 Rh2DG382900 Rh2DG623700 Rh5AG027500 Rh5CG029900
rosa_wichuraiana Rw2G031490 Rw2G049850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 218
AccI GTMKAC 1 cut(s) 283
AciI CCGC 1 cut(s) 617
AclWI GGATC 3 cut(s) 252, 336, 968
AcsI RAATTY 3 cut(s) 300, 930, 1163
AcuI CTGAAG 2 cut(s) 262, 483
AfaI GTAC 2 cut(s) 504, 1023
AfiI CCNNNNNNNGG 2 cut(s) 218, 1031
AflIII ACRYGT 1 cut(s) 1103
AgsI TTSAA 7 cut(s) 38, 145, 181, 346, 515, 832, 1279
AjnI CCWGG 1 cut(s) 170
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AluBI AGCT 7 cut(s) 8, 120, 184, 467, 968, 1091, 1267
AluI AGCT 7 cut(s) 8, 120, 184, 467, 968, 1091, 1267
Alw21I GWGCWC 1 cut(s) 1009
Alw26I GTCTC 3 cut(s) 172, 264, 857
AlwI GGATC 3 cut(s) 252, 336, 968
Ama87I CYCGRG 1 cut(s) 267
AoxI GGCC 1 cut(s) 594
ApoI RAATTY 3 cut(s) 300, 930, 1163
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 3 cut(s) 27, 396, 594
AsuII TTCGAA 1 cut(s) 1244
AvaI CYCGRG 1 cut(s) 267
AvaII GGWCC 2 cut(s) 27, 396
BbsI GAAGAC 3 cut(s) 557, 594, 617
Bbv12I GWGCWC 1 cut(s) 1009
BccI CCATC 2 cut(s) 67, 1143
BciT130I CCWGG 1 cut(s) 172
BcoDI GTCTC 3 cut(s) 172, 264, 857
BfaI CTAG 2 cut(s) 992, 1088
BfmI CTRYAG 1 cut(s) 543
BglII AGATCT 1 cut(s) 1084
BmcAI AGTACT 1 cut(s) 504
Bme1390I CCNGG 1 cut(s) 172
Bme18I GGWCC 2 cut(s) 27, 396
BmeT110I CYCGRG 1 cut(s) 267
BmgT120I GGNCC 3 cut(s) 27, 396, 594
BmrFI CCNGG 1 cut(s) 172
BmsI GCATC 1 cut(s) 31
BpiI GAAGAC 3 cut(s) 557, 594, 617
BpmI CTGGAG 2 cut(s) 154, 1257
Bpu14I TTCGAA 1 cut(s) 1244
BsaBI GATNNNNATC 1 cut(s) 641
BsaI GGTCTC 1 cut(s) 857
BsaJI CCNNGG 2 cut(s) 171, 527
BsaXI ACNNNNNCTCC 2 cut(s) 672, 702
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 1031
Bse8I GATNNNNATC 1 cut(s) 641
BseBI CCWGG 1 cut(s) 172
BseDI CCNNGG 2 cut(s) 171, 527
BseGI GGATG 3 cut(s) 78, 322, 819
BseJI GATNNNNATC 1 cut(s) 641
BseLI CCNNNNNNNGG 2 cut(s) 218, 1031
BseMII CTCAG 3 cut(s) 675, 684, 1022
BseRI GAGGAG 1 cut(s) 698
Bsh1285I CGRYCG 1 cut(s) 592
BshFI GGCC 1 cut(s) 596
BsiEI CGRYCG 1 cut(s) 592
BsiHKAI GWGCWC 1 cut(s) 1009
BsiHKCI CYCGRG 1 cut(s) 267
BslFI GGGAC 1 cut(s) 1092
BslI CCNNNNNNNGG 2 cut(s) 218, 1031
BsmAI GTCTC 3 cut(s) 172, 264, 857
BsmFI GGGAC 1 cut(s) 1092
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 1 cut(s) 596
Bso31I GGTCTC 1 cut(s) 857
BsoBI CYCGRG 1 cut(s) 267
Bsp119I TTCGAA 1 cut(s) 1244
Bsp1286I GDGCHC 1 cut(s) 1009
Bsp143I GATC 6 cut(s) 244, 328, 367, 735, 973, 1084
BspACI CCGC 1 cut(s) 617
BspANI GGCC 1 cut(s) 596
BspCNI CTCAG 3 cut(s) 674, 683, 1021
BspHI TCATGA 1 cut(s) 934
BspPI GGATC 3 cut(s) 252, 336, 968
BspT104I TTCGAA 1 cut(s) 1244
BspTNI GGTCTC 1 cut(s) 857
BssECI CCNNGG 2 cut(s) 171, 527
BssMI GATC 6 cut(s) 244, 328, 367, 735, 973, 1084
BssT1I CCWWGG 1 cut(s) 527
Bst2UI CCWGG 1 cut(s) 172
Bst4CI ACNGT 6 cut(s) 166, 490, 544, 586, 719, 758
Bst6I CTCTTC 2 cut(s) 389, 903
BstAPI GCANNNNNTGC 1 cut(s) 1298
BstBI TTCGAA 1 cut(s) 1244
BstC8I GCNNGC 2 cut(s) 534, 745
BstDEI CTNAG 3 cut(s) 661, 670, 1008
BstF5I GGATG 3 cut(s) 78, 322, 819
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 6 cut(s) 247, 331, 370, 738, 976, 1087
BstMAI GTCTC 3 cut(s) 172, 264, 857
BstMBI GATC 6 cut(s) 244, 328, 367, 735, 973, 1084
BstMCI CGRYCG 1 cut(s) 592
BstMWI GCNNNNNNNGC 1 cut(s) 1298
BstNI CCWGG 1 cut(s) 172
BstNSI RCATGY 3 cut(s) 635, 747, 1107
BstSCI CCNGG 1 cut(s) 170
BstSFI CTRYAG 1 cut(s) 543
BstV2I GAAGAC 3 cut(s) 557, 594, 617
BstX2I RGATCY 1 cut(s) 1084
BstYI RGATCY 1 cut(s) 1084
BsuRI GGCC 1 cut(s) 596
BtsCI GGATG 3 cut(s) 78, 322, 819
BtsIMutI CAGTG 2 cut(s) 162, 452
Cac8I GCNNGC 2 cut(s) 534, 745
CciI TCATGA 1 cut(s) 934
CfoI GCGC 1 cut(s) 211
Cfr13I GGNCC 3 cut(s) 27, 396, 594
CpoI CGGWCCG 1 cut(s) 396
Csp6I GTAC 2 cut(s) 503, 1022
CspI CGGWCCG 1 cut(s) 396
CviAII CATG 6 cut(s) 598, 632, 744, 935, 1104, 1177
CviQI GTAC 2 cut(s) 503, 1022
DdeI CTNAG 3 cut(s) 661, 670, 1008
DpnI GATC 6 cut(s) 246, 330, 369, 737, 975, 1086
DpnII GATC 6 cut(s) 244, 328, 367, 735, 973, 1084
Eam1104I CTCTTC 2 cut(s) 389, 903
EarI CTCTTC 2 cut(s) 389, 903
Eco130I CCWWGG 1 cut(s) 527
Eco31I GGTCTC 1 cut(s) 857
Eco47I GGWCC 2 cut(s) 27, 396
Eco57I CTGAAG 2 cut(s) 262, 483
Eco88I CYCGRG 1 cut(s) 267
EcoRII CCWGG 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 527
ErhI CCWWGG 1 cut(s) 527
FaeI CATG 6 cut(s) 601, 635, 747, 938, 1107, 1180
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FaqI GGGAC 1 cut(s) 1092
FatI CATG 6 cut(s) 597, 631, 743, 934, 1103, 1176
FblI GTMKAC 1 cut(s) 283
FokI GGATG 3 cut(s) 85, 329, 826
FspBI CTAG 2 cut(s) 992, 1088
GlaI GCGC 1 cut(s) 210
GsuI CTGGAG 2 cut(s) 154, 1257
HaeIII GGCC 1 cut(s) 596
HhaI GCGC 1 cut(s) 211
Hin1II CATG 6 cut(s) 601, 635, 747, 938, 1107, 1180
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HindIII AAGCTT 1 cut(s) 465
HinfI GANTC 2 cut(s) 333, 613
Hpy166II GTNNAC 4 cut(s) 284, 413, 722, 916
Hpy188I TCNGA 7 cut(s) 367, 400, 429, 463, 664, 673, 973
Hpy188III TCNNGA 5 cut(s) 269, 770, 935, 947, 1236
Hpy8I GTNNAC 4 cut(s) 284, 413, 722, 916
HpyAV CCTTC 2 cut(s) 987, 1242
HpyCH4III ACNGT 6 cut(s) 166, 490, 544, 586, 719, 758
HpyCH4IV ACGT 2 cut(s) 415, 918
HpyCH4V TGCA 4 cut(s) 44, 997, 1256, 1292
HpyF10VI GCNNNNNNNGC 1 cut(s) 1298
HpyF3I CTNAG 3 cut(s) 661, 670, 1008
HpySE526I ACGT 2 cut(s) 415, 918
Hsp92II CATG 6 cut(s) 601, 635, 747, 938, 1107, 1180
HspAI GCGC 1 cut(s) 209
Kzo9I GATC 6 cut(s) 244, 328, 367, 735, 973, 1084
LmnI GCTCC 2 cut(s) 125, 377
LpnPI CCDG 9 cut(s) 157, 170, 184, 225, 531, 783, 987, 1194, 1221
LweI GCATC 1 cut(s) 31
MaeI CTAG 2 cut(s) 992, 1088
MaeII ACGT 2 cut(s) 415, 918
MaeIII GTNAC 3 cut(s) 160, 230, 378
MalI GATC 6 cut(s) 246, 330, 369, 737, 975, 1086
MboI GATC 6 cut(s) 244, 328, 367, 735, 973, 1084
MflI RGATCY 1 cut(s) 1084
MhlI GDGCHC 1 cut(s) 1009
MluCI AATT 9 cut(s) 145, 300, 347, 360, 930, 942, 1072, 1163, 1270
MlyI GAGTC 1 cut(s) 607
MmeI TCCRAC 8 cut(s) 199, 294, 472, 505, 791, 885, 901, 1176
MnlI CCTC 9 cut(s) 13, 71, 107, 235, 670, 676, 679, 814, 904
MseI TTAA 6 cut(s) 114, 438, 578, 1017, 1071, 1080
MslI CAYNNNNRTG 1 cut(s) 1102
MspR9I CCNGG 1 cut(s) 172
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 1 cut(s) 172
MwoI GCNNNNNNNGC 1 cut(s) 1298
NdeII GATC 6 cut(s) 244, 328, 367, 735, 973, 1084
NlaIII CATG 6 cut(s) 601, 635, 747, 938, 1107, 1180
NmuCI GTSAC 2 cut(s) 160, 230
NspI RCATGY 3 cut(s) 635, 747, 1107
NspV TTCGAA 1 cut(s) 1244
PaeI GCATGC 1 cut(s) 747
PaeR7I CTCGAG 1 cut(s) 267
PagI TCATGA 1 cut(s) 934
PciI ACATGT 1 cut(s) 1103
PctI GAATGC 1 cut(s) 354
PfeI GAWTC 1 cut(s) 333
PflMI CCANNNNNTGG 1 cut(s) 218
PleI GAGTC 1 cut(s) 607
PpsI GAGTC 1 cut(s) 607
PscI ACATGT 1 cut(s) 1103
Psp6I CCWGG 1 cut(s) 170
PspGI CCWGG 1 cut(s) 170
PspPI GGNCC 3 cut(s) 27, 396, 594
PsuI RGATCY 1 cut(s) 1084
RsaI GTAC 2 cut(s) 504, 1023
RsaNI GTAC 2 cut(s) 503, 1022
RseI CAYNNNNRTG 1 cut(s) 1102
Rsr2I CGGWCCG 1 cut(s) 396
RsrII CGGWCCG 1 cut(s) 396
SaqAI TTAA 6 cut(s) 114, 438, 578, 1017, 1071, 1080
Sau3AI GATC 6 cut(s) 244, 328, 367, 735, 973, 1084
Sau96I GGNCC 3 cut(s) 27, 396, 594
ScaI AGTACT 1 cut(s) 504
SchI GAGTC 1 cut(s) 607
ScrFI CCNGG 1 cut(s) 172
SduI GDGCHC 1 cut(s) 1009
SfaNI GCATC 1 cut(s) 31
SfcI CTRYAG 1 cut(s) 543
Sfr274I CTCGAG 1 cut(s) 267
SfuI TTCGAA 1 cut(s) 1244
SinI GGWCC 2 cut(s) 27, 396
SlaI CTCGAG 1 cut(s) 267
SmiMI CAYNNNNRTG 1 cut(s) 1102
SmlI CTYRAG 1 cut(s) 267
SmoI CTYRAG 1 cut(s) 267
SphI GCATGC 1 cut(s) 747
Sse9I AATT 9 cut(s) 145, 300, 347, 360, 930, 942, 1072, 1163, 1270
SsiI CCGC 1 cut(s) 617
SspI AATATT 1 cut(s) 836
SspMI CTAG 2 cut(s) 992, 1088
StyD4I CCNGG 1 cut(s) 170
StyI CCWWGG 1 cut(s) 527
TaaI ACNGT 6 cut(s) 166, 490, 544, 586, 719, 758
TaiI ACGT 2 cut(s) 418, 921
TaqI TCGA 5 cut(s) 268, 331, 336, 1049, 1244
TaqII GACCGA 2 cut(s) 384, 578
TasI AATT 9 cut(s) 145, 300, 347, 360, 930, 942, 1072, 1163, 1270
TatI WGTACW 1 cut(s) 502
TfiI GAWTC 1 cut(s) 333
Tru1I TTAA 6 cut(s) 114, 438, 578, 1017, 1071, 1080
Tru9I TTAA 6 cut(s) 114, 438, 578, 1017, 1071, 1080
TscAI CASTG 2 cut(s) 169, 452
TseFI GTSAC 2 cut(s) 160, 230
Tsp45I GTSAC 2 cut(s) 160, 230
TspDTI ATGAA 4 cut(s) 513, 923, 1050, 1165
TspRI CASTG 2 cut(s) 169, 452
Van91I CCANNNNNTGG 1 cut(s) 218
VpaK11BI GGWCC 2 cut(s) 27, 396
XapI RAATTY 3 cut(s) 300, 930, 1163
XceI RCATGY 3 cut(s) 635, 747, 1107
XcmI CCANNNNNNNNNTGG 1 cut(s) 488
XhoI CTCGAG 1 cut(s) 267
XmiI GTMKAC 1 cut(s) 283
XspI CTAG 2 cut(s) 992, 1088
ZrmI AGTACT 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.