MD09G1055800.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000363
Physical Location & Seq
Reverse (-)
3700657 .. 3703464
2808 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1055800.v1.1.491

Sequence Viewer

Length: 2808 bp
ATGGGTAACATTGTCTCAATATCCCTGCCAAGCGATCCCATTTTTGATTCTTCCTGCTGGGGTCCCCTTATTCAACCACCAAAATACATAAGGAGGCTGAAAAAAAACCTCGAAGCTCTGGATCGTTCTTTTCGAGAACTGACCGCTTTGAAGGTTGATGTATTGAGAAAGGTTGAGGTTGCAGAGCTGCAGAGATCCATGAAGCGATTGAACCAGGTACAACTGTGGATTGAAAAGGTGGAAAAGATTGAGAATGAAGTCGCAGAAGTCAAGCGTGGTCGAGTTCAAGAAATTCAGAAATTGTGTTGCGGAGCTTACTGCTCAAAAAACTACTTGTCCAGCTACAAGTACGGAAAAAAAGTGTATAAAAAGCTGGCAGAAGTCGATGATTTAAAGAACAAGGGTATTTTTGCGGAGGTCGCAGAGGAAACCTTCCCGGCAACTTTAGTCCGTGAAAGACCTATGGAACCAACTGTTGGCATGGAATTTGTGTTTTATAAGGTTTGGAGCCAGCTAGAAGATGAACATGTGGGAATAATTGGATTGTACGGGATGGGAGGGGTGGGTAAGACTACCCTACTGACCCAAATCAATAACAAATTGCTCCATGCTGATTTTGATCTCGTGATATGGATTGTGGTATCCAAGGACCACAATGTTGAAACTGTCCAAGATAAGATTGGTGACAAGATTGGGTTTTCCAGCAACTCGTGGAAACAAAAACAGCAGTCAGACAAAGCTGAACACATCTGCAGGCTATTGAGCAAGAAGAAGTTTGTGTTATTGTTCGATGATATATGGGAGCCTATTGAAATAACCAAATTAGGGGTTCCTATTCCTAACCCGCACAACAAGTCTAAAATAATCTTCACCACTCGTTCGGAGGATGTATGTGGTCAGATGGATGCTCATAAGAAGATTAAAGTGGAGTGTTTGGCATGGGACAAAGCATGGAACTTGTTTCAGGAGAAGGTTGGGAGGGAAACACTTGGTATTCACCCAGATATTCAACGACTTGCTCAGACTGTTGCAAAAGAGTGTGGAGGTTTGCCACTCGCACTAATTACTGTTGGTCGGGCCATGGCTTGCAAGAAAACAACTCAAGAGTGGAACCATGCGATTCACATTCTGAAAAAATCCGCGCACGAGTTTTCAGGTATGGAGGATAAGGTGTTCCGTCTTTTAAAATTTAGTTATGATAATTTAGCCGATGAGAAGGTCAGATCTTGCTTCTTATATTGTGCTCTATATCCAGAAGATTTCTTCATATTTAAAGATGATTTAGTATATCTTTGGATGTGTGAGGCAATGTTGGACGAATATGGGAGTGTAGAGGAAGCAAAAAACAAGTGTTACGATATTATAGGAACTCTTGTTGCTGCATGTTTGTTGGAAGTTAACTTAGATATGGTAAAGATGCATGATATGATTCGTGACATGGCATTGTGGTTAGCTCGTGACTGTTCAAAAGAGAACGACAGTTTCCTTGTCCATACAAGTGCCCAAGCAGCGCCGGATGTCAAAAAATGGAAAACCGCAACAAGGGTGTCGTTGGTAGATAATCATATTGAAAGTATCGTTGAAACACCAAAATCTACCCAGTTGTTGACCTTATTGATTCAAGGAAGTTATATTAGCAGAAGTCCTCTCAATATGATTGTTGATGGCTTCTTCGATTACATGCATAAACTGCGAGTTTTGGATTTGTCTAGAAATGAGAAGCTAACCCAATTGCCATCTGGAGTTTCAAACCTGATTGCCTTACAGCATCTGAATTTGTCTAGGACTGGTATAAGAGAATTGTCGATCGAATTGAAGGCCTTAGTGAGGCTAAAATATTTGAACTTGGAGTATACAAATCAACTTAATTTTGTTCCACCAGAACTAATATCAAATTTTCACATGCTAAAAGTTCTGAAATTGTTCGGCTGTAGTTCTTCAGACAGAATTCTTTTTGGTGGTGAAAGAGCCCTGATAGAGGAAATCGAGGGTTTGAAACACCTTGAGGTATTTACTTTGTGCATACGAAGTAGCTATTGTTTCAAAAAATTGTTCAGCTACAACATAATAGTGACCAGCACTCAGTATCTACACCTCTCGGATGGCAAGAACTACTCAAGCGTTCTAGATATATCATCTCTGGCGGACATGAAACATCTTCGTGGCCTTCATATTGATGAATATCCTAACCTGGAAGAATTGGAGATTTATTGGGCAGGACAAGATCCTAATGATCCTCGGAACGCGATGATGAGAAACCAAAGTCGCTTCTGTGGCCTTGAGTACGTAGATGTATGCCGGTGCCCAAATCTCAAGGACCTGACATGGCTCGTTTTCGCGTCCAATCTCAGAATCTTAGAAGTGCGTGACTGCTCTGGGATGGAGAAAATAATCGATTTGGAAAGATTTGGTAGAATGGCAGGTGCGGTGAAAGAACAAAATGCTTTCGCAAAACTTACAGATCTGCATTTGGATTCACTACCGCGGCTAAGGAGCATATATGGCAATGCCTTACCATTTCTGCATCTCAACCAAATCTTGGTATATGAATGTCCGGCGTTGAAGAAGCTGCCACTCAACTCTACCAGTGCCGAACGATGTAATCTCATTATCAGGGGACAGAAAGAGTGGTGGAATGGCTTAGAATGGGAGGATGAAGTTTCTAGAAGTGTTTTTCTTCCCTACTTCTTGTTAGAATTCGGAGAGGTTCTCAGAACAGATTTCAAAGAAACTGATTTGGGGGAATTTTCAGATCATCTATTATTCTTCTCCGCAAGGAGGAAATATATATTGTACAAAGACTTGTAA

Protein Analysis

936

Amino Acids

107.62

Weight (kDa)

6.91

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 167 - 330 1.4e-49 NB-ARC domain
ATPase_2 PF01637 172 - 356 7.6e-06 ATPase domain predominantly from Archaea
WHD_DRP PF23559 416 - 481 1.3e-06 Disease resistance protein Winged helix domain
LRR_8 PF13855 545 - 597 5.4e-07 Leucine rich repeat
LRR_14 PF23598 561 - 729 4.8e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 753 - 857 7.1e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12210 AT1G12210 AT1G12210 AT1G12220 AT1G12220 AT1G12220 AT1G12280 AT1G12280 AT1G12290 AT1G12290 AT1G12290 AT1G12290 AT1G15890 AT1G15890 AT1G51480 AT1G52660 AT1G52660 AT1G61180 AT1G61180 AT1G61190 AT1G61190 AT1G61190 AT1G61190 AT1G61300 AT1G61300 AT1G61310 AT3G15700 AT3G15700 AT4G10780 AT4G10780 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43740 AT5G43740 AT5G63020
fragaria_vesca FvH4_6g30050 FvH4_6g48220
malus_domestica MD09G1055800.v1.1 MD09G1198800.v1.1 MD17G1204800.v1.1 MD17G1204900.v1.1 MD17G1205000.v1.1 MD17G1205300.v1.1 MD17G1205700.v1.1 MD17G1205800.v1.1 MD17G1224100.v1.1
prunus_persica Prupe.3G054800_v2.0.a1 Prupe.3G054800_v2.0.a1 Prupe.3G080300_v2.0.a1 Prupe.3G080400_v2.0.a1 Prupe.3G080500_v2.0.a1 Prupe.3G080600_v2.0.a1 Prupe.3G080800_v2.0.a1 Prupe.3G083200_v2.0.a1 Prupe.3G099800_v2.0.a1 Prupe.3G099800_v2.0.a1
pyrus_communis pycom17g20860 pycom17g20910 pycom17g22790
rosa_chinensis RchiOBHm_Chr2g0136891 RchiOBHm_Chr2g0136901 RchiOBHm_Chr2g0167711 RchiOBHm_Chr2g0167721 RchiOBHm_Chr2g0167731 RchiOBHm_Chr4g0407611
rosa_laevigata RLG00000018943 RLG00000019278 RLG00000019282 RLG00000019587 RLG00000021748 RLG00000021749 RLG00000031057
rosa_multiflora Rmu_sc0002143.1_g000003 Rmu_sc0002782.1_g000001 Rmu_sc0023879.1_g000001 Rmu_sc0042171.1_g000001
rosa_roxburghii Rroxscaffold_2G00083300 Rroxscaffold_2G00107890 Rroxscaffold_2G00116680
rosa_rugosa Rorug02G0271700 Rorug02G0334900 Rorug02G0531800 Rorug02G0531800
rosa_samantha Rh2AG359900 Rh2AG385100 Rh2AG600500 Rh2AG600600 Rh2BG392200 Rh2BG611000 Rh2BG611100 Rh2BG611200 Rh2CG343100 Rh2CG343300 Rh2CG372300 Rh2CG581400 Rh2CG581500 Rh2DG382900 Rh2DG623700 Rh5AG027500 Rh5CG029900
rosa_wichuraiana Rw2G031490 Rw2G049850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 498
AarI CACCTGC 1 cut(s) 2411
Acc36I ACCTGC 1 cut(s) 2411
AccB1I GGYRCC 1 cut(s) 2301
AccB7I CCANNNNNTGG 2 cut(s) 476, 2539
AccI GTMKAC 1 cut(s) 1853
AccII CGCG 4 cut(s) 1142, 2246, 2339, 2485
AclWI GGATC 5 cut(s) 29, 129, 189, 2219, 2228
AcsI RAATTY 8 cut(s) 291, 485, 1187, 1774, 1894, 1947, 2696, 2744
AcuI CTGAAG 1 cut(s) 1923
AfaI GTAC 5 cut(s) 219, 350, 548, 2285, 2795
AfiI CCNNNNNNNGG 7 cut(s) 476, 825, 1542, 1827, 1978, 2539, 2778
AflIII ACRYGT 1 cut(s) 526
AjnI CCWGG 2 cut(s) 213, 2190
AjuI GAANNNNNNNTTGG 2 cut(s) 459, 491
AloI GAACNNNNNNTCC 2 cut(s) 1157, 1189
Alw21I GWGCWC 1 cut(s) 1246
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 5 cut(s) 29, 129, 189, 2219, 2228
AlwNI CAGNNNCTG 1 cut(s) 1771
AoxI GGCC 4 cut(s) 1077, 1818, 2164, 2275
ApeKI GCWGC 4 cut(s) 187, 1379, 1508, 2569
ApoI RAATTY 8 cut(s) 291, 485, 1187, 1774, 1894, 1947, 2696, 2744
Asp700I GAANNNNTTC 1 cut(s) 1922
AspLEI GCGC 2 cut(s) 1144, 1513
AspS9I GGNCC 4 cut(s) 62, 649, 1077, 2317
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 5 cut(s) 695, 862, 989, 1973, 2440
AvaII GGWCC 3 cut(s) 62, 649, 2317
BaeGI GKGCMC 2 cut(s) 1504, 2306
BaeI ACNNNNGTAYC 2 cut(s) 209, 242
BanI GGYRCC 1 cut(s) 2301
BanII GRGCYC 1 cut(s) 1972
BauI CACGAG 4 cut(s) 623, 709, 1145, 1455
Bbv12I GWGCWC 1 cut(s) 1246
BbvI GCAGC 4 cut(s) 174, 1366, 1520, 2556
BccI CCATC 6 cut(s) 547, 895, 1658, 1744, 2096, 2374
BciT130I CCWGG 2 cut(s) 215, 2192
BciVI GTATCC 1 cut(s) 652
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 1 cut(s) 19
BfaI CTAG 5 cut(s) 515, 1710, 1782, 2126, 2664
BfmI CTRYAG 3 cut(s) 188, 751, 1930
BfoI RGCGCY 1 cut(s) 1514
BfuAI ACCTGC 1 cut(s) 2411
BfuI GTATCC 1 cut(s) 652
BglII AGATCT 2 cut(s) 1223, 2461
BisI GCNGC 5 cut(s) 188, 1380, 1509, 2486, 2570
BlsI GCNGC 5 cut(s) 189, 1381, 1510, 2487, 2571
Bme1390I CCNGG 3 cut(s) 215, 437, 2192
Bme18I GGWCC 3 cut(s) 62, 649, 2317
BmgT120I GGNCC 4 cut(s) 62, 649, 1077, 2317
BmiI GGNNCC 8 cut(s) 63, 64, 468, 509, 804, 831, 1112, 2303
BmrFI CCNGG 3 cut(s) 215, 437, 2192
BmrI ACTGGG 1 cut(s) 1594
BmsI GCATC 4 cut(s) 895, 1407, 1777, 2533
BmuI ACTGGG 1 cut(s) 1594
BplI GAGNNNNNCTC 2 cut(s) 2694, 2726
BpmI CTGGAG 1 cut(s) 1761
Bpu10I CCTNAGC 1 cut(s) 2489
BpuEI CTTGAG 5 cut(s) 1086, 2024, 2101, 2297, 2300
BpuMI CCSGG 1 cut(s) 437
Bsa29I ATCGAT 1 cut(s) 2394
BsaAI YACGTR 1 cut(s) 2287
BsaBI GATNNNNATC 2 cut(s) 618, 2181
BsaJI CCNNGG 4 cut(s) 645, 1080, 2237, 2483
Bsc4I CCNNNNNNNGG 7 cut(s) 476, 825, 1542, 1827, 1978, 2539, 2778
Bse118I RCCGGY 1 cut(s) 2298
Bse1I ACTGG 3 cut(s) 1600, 1792, 2586
Bse3DI GCAATG 2 cut(s) 1314, 2512
Bse8I GATNNNNATC 2 cut(s) 618, 2181
BseBI CCWGG 2 cut(s) 215, 2192
BseCI ATCGAT 1 cut(s) 2394
BseDI CCNNGG 4 cut(s) 645, 1080, 2237, 2483
BseGI GGATG 8 cut(s) 558, 892, 910, 1302, 1522, 2107, 2385, 2659
BseJI GATNNNNATC 2 cut(s) 618, 2181
BseLI CCNNNNNNNGG 7 cut(s) 476, 825, 1542, 1827, 1978, 2539, 2778
BseMI GCAATG 2 cut(s) 1314, 2512
BseMII CTCAG 4 cut(s) 1034, 2096, 2362, 2725
BseNI ACTGG 3 cut(s) 1600, 1792, 2586
BseSI GKGCMC 2 cut(s) 1504, 2306
BseXI GCAGC 4 cut(s) 174, 1366, 1520, 2556
BseYI CCCAGC 1 cut(s) 57
Bsh1236I CGCG 4 cut(s) 1142, 2246, 2339, 2485
Bsh1285I CGRYCG 1 cut(s) 1809
BshFI GGCC 4 cut(s) 1079, 1820, 2166, 2277
BshNI GGYRCC 1 cut(s) 2301
BshVI ATCGAT 1 cut(s) 2394
BsiEI CGRYCG 1 cut(s) 1809
BsiHKAI GWGCWC 1 cut(s) 1246
BsiSI CCGG 4 cut(s) 437, 1514, 2299, 2555
BslFI GGGAC 3 cut(s) 48, 956, 2631
BslI CCNNNNNNNGG 7 cut(s) 476, 825, 1542, 1827, 1978, 2539, 2778
BsmAI GTCTC 1 cut(s) 19
BsmFI GGGAC 3 cut(s) 48, 956, 2631
BsnI GGCC 4 cut(s) 1079, 1820, 2166, 2277
Bsp1286I GDGCHC 4 cut(s) 1246, 1504, 1972, 2306
Bsp1407I TGTACA 1 cut(s) 2793
Bsp19I CCATGG 1 cut(s) 1080
BspANI GGCC 4 cut(s) 1079, 1820, 2166, 2277
BspCNI CTCAG 4 cut(s) 1033, 2095, 2361, 2724
BspDI ATCGAT 1 cut(s) 2394
BspFNI CGCG 4 cut(s) 1142, 2246, 2339, 2485
BspLI GGNNCC 8 cut(s) 63, 64, 468, 509, 804, 831, 1112, 2303
BspMAI CTGCAG 2 cut(s) 192, 755
BspMI ACCTGC 1 cut(s) 2411
BspPI GGATC 5 cut(s) 29, 129, 189, 2219, 2228
BspT107I GGYRCC 1 cut(s) 2301
BsrDI GCAATG 2 cut(s) 1314, 2512
BsrFI RCCGGY 1 cut(s) 2298
BsrGI TGTACA 1 cut(s) 2793
BsrI ACTGG 3 cut(s) 1600, 1792, 2586
BssAI RCCGGY 1 cut(s) 2298
BssECI CCNNGG 4 cut(s) 645, 1080, 2237, 2483
BssNAI GTATAC 1 cut(s) 1854
BssSI CACGAG 4 cut(s) 623, 709, 1145, 1455
BssT1I CCWWGG 2 cut(s) 645, 1080
Bst1107I GTATAC 1 cut(s) 1854
Bst2BI CACGAG 4 cut(s) 623, 709, 1145, 1455
Bst2UI CCWGG 2 cut(s) 215, 2192
Bst4CI ACNGT 7 cut(s) 225, 475, 667, 1027, 1069, 1463, 1481
BstAPI GCANNNNNTGC 1 cut(s) 1690
BstAUI TGTACA 1 cut(s) 2793
BstBAI YACGTR 1 cut(s) 2287
BstC8I GCNNGC 4 cut(s) 375, 512, 755, 1087
BstDEI CTNAG 9 cut(s) 1020, 1402, 1822, 2082, 2348, 2356, 2489, 2641, 2711
BstDSI CCRYGG 2 cut(s) 1080, 2483
BstENI CCTNNNNNAGG 2 cut(s) 1825, 1976
BstF5I GGATG 8 cut(s) 558, 892, 910, 1302, 1522, 2107, 2385, 2659
BstFNI CGCG 4 cut(s) 1142, 2246, 2339, 2485
BstH2I RGCGCY 1 cut(s) 1514
BstHHI GCGC 2 cut(s) 1144, 1513
BstMAI GTCTC 1 cut(s) 19
BstMCI CGRYCG 1 cut(s) 1809
BstMWI GCNNNNNNNGC 5 cut(s) 419, 1508, 1690, 2274, 2502
BstNI CCWGG 2 cut(s) 215, 2192
BstNSI RCATGY 4 cut(s) 530, 1386, 1684, 1906
BstSCI CCNGG 3 cut(s) 213, 435, 2190
BstSFI CTRYAG 3 cut(s) 188, 751, 1930
BstSLI GKGCMC 2 cut(s) 1504, 2306
BstSNI TACGTA 1 cut(s) 2287
BstUI CGCG 4 cut(s) 1142, 2246, 2339, 2485
BstV1I GCAGC 4 cut(s) 174, 1366, 1520, 2556
BstX2I RGATCY 4 cut(s) 194, 1223, 2224, 2461
BstYI RGATCY 4 cut(s) 194, 1223, 2224, 2461
BstZ17I GTATAC 1 cut(s) 1854
Bsu15I ATCGAT 1 cut(s) 2394
BsuI GTATCC 1 cut(s) 652
BsuRI GGCC 4 cut(s) 1079, 1820, 2166, 2277
BsuTUI ATCGAT 1 cut(s) 2394
BtgI CCRYGG 2 cut(s) 1080, 2483
BtgZI GCGATG 1 cut(s) 2261
BtsCI GGATG 8 cut(s) 558, 892, 910, 1302, 1522, 2107, 2385, 2659
BtsIMutI CAGTG 1 cut(s) 2593
BveI ACCTGC 1 cut(s) 2411
Cac8I GCNNGC 4 cut(s) 375, 512, 755, 1087
CaiI CAGNNNCTG 1 cut(s) 1771
CfoI GCGC 2 cut(s) 1144, 1513
Cfr10I RCCGGY 1 cut(s) 2298
Cfr13I GGNCC 4 cut(s) 62, 649, 1077, 2317
Cfr42I CCGCGG 1 cut(s) 2486
ClaI ATCGAT 1 cut(s) 2394
CseI GACGC 1 cut(s) 2328
CsiI ACCWGGT 1 cut(s) 213
Csp6I GTAC 5 cut(s) 218, 349, 547, 2284, 2794
CviQI GTAC 5 cut(s) 218, 349, 547, 2284, 2794
DdeI CTNAG 9 cut(s) 1020, 1402, 1822, 2082, 2348, 2356, 2489, 2641, 2711
DraI TTTAAA 3 cut(s) 393, 1185, 1273
EciI GGCGGA 1 cut(s) 2159
Eco105I TACGTA 1 cut(s) 2287
Eco130I CCWWGG 2 cut(s) 645, 1080
Eco147I AGGCCT 1 cut(s) 1820
Eco24I GRGCYC 1 cut(s) 1972
Eco47I GGWCC 3 cut(s) 62, 649, 2317
Eco57I CTGAAG 1 cut(s) 1923
EcoNI CCTNNNNNAGG 2 cut(s) 1825, 1976
EcoO109I RGGNCCY 2 cut(s) 62, 2317
EcoRI GAATTC 2 cut(s) 1947, 2696
EcoRII CCWGG 2 cut(s) 213, 2190
EcoT14I CCWWGG 2 cut(s) 645, 1080
EcoT22I ATGCAT 2 cut(s) 1422, 1686
EcoT38I GRGCYC 1 cut(s) 1972
ErhI CCWWGG 2 cut(s) 645, 1080
FaqI GGGAC 3 cut(s) 48, 956, 2631
FauI CCCGC 1 cut(s) 852
FblI GTMKAC 1 cut(s) 1853
Fnu4HI GCNGC 5 cut(s) 188, 1380, 1509, 2486, 2570
FokI GGATG 8 cut(s) 565, 899, 917, 1309, 1529, 2114, 2392, 2666
FriOI GRGCYC 1 cut(s) 1972
Fsp4HI GCNGC 5 cut(s) 188, 1380, 1509, 2486, 2570
FspBI CTAG 5 cut(s) 515, 1710, 1782, 2126, 2664
GlaI GCGC 2 cut(s) 1143, 1512
GluI GCNGC 5 cut(s) 188, 1380, 1509, 2486, 2570
GsaI CCCAGC 1 cut(s) 61
GsuI CTGGAG 1 cut(s) 1761
HaeII RGCGCY 1 cut(s) 1514
HaeIII GGCC 4 cut(s) 1079, 1820, 2166, 2277
HapII CCGG 4 cut(s) 437, 1514, 2299, 2555
HgaI GACGC 1 cut(s) 2328
HhaI GCGC 2 cut(s) 1144, 1513
Hin6I GCGC 2 cut(s) 1142, 1511
HinP1I GCGC 2 cut(s) 1142, 1511
HincII GTYRAC 2 cut(s) 1399, 1608
HindII GTYRAC 2 cut(s) 1399, 1608
HinfI GANTC 6 cut(s) 47, 1120, 1429, 1618, 2352, 2474
HpaI GTTAAC 1 cut(s) 1399
HpaII CCGG 4 cut(s) 437, 1514, 2299, 2555
HphI GGTGA 5 cut(s) 695, 862, 989, 1973, 2440
Hpy166II GTNNAC 3 cut(s) 1399, 1608, 1854
Hpy8I GTNNAC 3 cut(s) 1399, 1608, 1854
HpyAV CCTTC 6 cut(s) 145, 442, 964, 1210, 1810, 2177
HpyCH4III ACNGT 7 cut(s) 225, 475, 667, 1027, 1069, 1463, 1481
HpyCH4IV ACGT 1 cut(s) 2286
HpyF10VI GCNNNNNNNGC 5 cut(s) 419, 1508, 1690, 2274, 2502
HpyF3I CTNAG 9 cut(s) 1020, 1402, 1822, 2082, 2348, 2356, 2489, 2641, 2711
HpySE526I ACGT 1 cut(s) 2286
HspAI GCGC 2 cut(s) 1142, 1511
KflI GGGWCCC 1 cut(s) 62
KspAI GTTAAC 1 cut(s) 1399
KspI CCGCGG 1 cut(s) 2486
LmnI GCTCC 5 cut(s) 311, 507, 609, 802, 2493
Lsp1109I GCAGC 4 cut(s) 174, 1366, 1520, 2556
LweI GCATC 4 cut(s) 895, 1407, 1777, 2533
MabI ACCWGGT 1 cut(s) 213
MaeI CTAG 5 cut(s) 515, 1710, 1782, 2126, 2664
MaeII ACGT 1 cut(s) 2286
MaeIII GTNAC 7 cut(s) 5, 683, 1352, 1433, 1457, 2071, 2366
MfeI CAATTG 1 cut(s) 1730
MflI RGATCY 4 cut(s) 194, 1223, 2224, 2461
MhlI GDGCHC 4 cut(s) 1246, 1504, 1972, 2306
MmeI TCCRAC 2 cut(s) 1293, 1371
Mph1103I ATGCAT 2 cut(s) 1422, 1686
MroXI GAANNNNTTC 1 cut(s) 1922
MseI TTAA 6 cut(s) 392, 921, 1184, 1272, 1398, 1866
MslI CAYNNNNRTG 4 cut(s) 1497, 2069, 2160, 2175
MspA1I CMGCKG 1 cut(s) 2485
MspI CCGG 4 cut(s) 437, 1514, 2299, 2555
MspR9I CCNGG 3 cut(s) 215, 437, 2192
MunI CAATTG 1 cut(s) 1730
MvaI CCWGG 2 cut(s) 215, 2192
MvnI CGCG 4 cut(s) 1142, 2246, 2339, 2485
MwoI GCNNNNNNNGC 5 cut(s) 419, 1508, 1690, 2274, 2502
NciI CCSGG 1 cut(s) 437
NcoI CCATGG 1 cut(s) 1080
NlaIV GGNNCC 8 cut(s) 63, 64, 468, 509, 804, 831, 1112, 2303
NmuCI GTSAC 5 cut(s) 683, 1433, 1457, 2071, 2366
NsiI ATGCAT 2 cut(s) 1422, 1686
NspI RCATGY 4 cut(s) 530, 1386, 1684, 1906
PaqCI CACCTGC 1 cut(s) 2411
PceI AGGCCT 1 cut(s) 1820
PciI ACATGT 1 cut(s) 526
PcsI WCGNNNNNNNCGW 1 cut(s) 130
PdmI GAANNNNTTC 1 cut(s) 1922
PfeI GAWTC 6 cut(s) 47, 1120, 1429, 1618, 2352, 2474
PflMI CCANNNNNTGG 2 cut(s) 476, 2539
PkrI GCNGC 5 cut(s) 189, 1381, 1510, 2487, 2571
Ple19I CGATCG 1 cut(s) 1809
Ppu21I YACGTR 1 cut(s) 2287
PpuMI RGGWCCY 2 cut(s) 62, 2317
PscI ACATGT 1 cut(s) 526
PsiI TTATAA 1 cut(s) 498
Psp5II RGGWCCY 2 cut(s) 62, 2317
Psp6I CCWGG 2 cut(s) 213, 2190
PspFI CCCAGC 1 cut(s) 57
PspGI CCWGG 2 cut(s) 213, 2190
PspN4I GGNNCC 8 cut(s) 63, 64, 468, 509, 804, 831, 1112, 2303
PspPI GGNCC 4 cut(s) 62, 649, 1077, 2317
PspPPI RGGWCCY 2 cut(s) 62, 2317
PstI CTGCAG 2 cut(s) 192, 755
PstNI CAGNNNCTG 1 cut(s) 1771
PsuI RGATCY 4 cut(s) 194, 1223, 2224, 2461
PvuI CGATCG 1 cut(s) 1809
RsaI GTAC 5 cut(s) 219, 350, 548, 2285, 2795
RsaNI GTAC 5 cut(s) 218, 349, 547, 2284, 2794
RseI CAYNNNNRTG 4 cut(s) 1497, 2069, 2160, 2175
SacII CCGCGG 1 cut(s) 2486
SaqAI TTAA 6 cut(s) 392, 921, 1184, 1272, 1398, 1866
SatI GCNGC 5 cut(s) 188, 1380, 1509, 2486, 2570
Sau96I GGNCC 4 cut(s) 62, 649, 1077, 2317
ScrFI CCNGG 3 cut(s) 215, 437, 2192
SduI GDGCHC 4 cut(s) 1246, 1504, 1972, 2306
SexAI ACCWGGT 1 cut(s) 213
SfaNI GCATC 4 cut(s) 895, 1407, 1777, 2533
SfcI CTRYAG 3 cut(s) 188, 751, 1930
Sfr303I CCGCGG 1 cut(s) 2486
SgrBI CCGCGG 1 cut(s) 2486
SinI GGWCC 3 cut(s) 62, 649, 2317
SmiMI CAYNNNNRTG 4 cut(s) 1497, 2069, 2160, 2175
SmlI CTYRAG 5 cut(s) 1101, 2003, 2116, 2279, 2312
SmoI CTYRAG 5 cut(s) 1101, 2003, 2116, 2279, 2312
SnaBI TACGTA 1 cut(s) 2287
SseBI AGGCCT 1 cut(s) 1820
SspI AATATT 1 cut(s) 1838
SspMI CTAG 5 cut(s) 515, 1710, 1782, 2126, 2664
StuI AGGCCT 1 cut(s) 1820
StyD4I CCNGG 3 cut(s) 213, 435, 2190
StyI CCWWGG 2 cut(s) 645, 1080
TaaI ACNGT 7 cut(s) 225, 475, 667, 1027, 1069, 1463, 1481
TaiI ACGT 1 cut(s) 2289
TatI WGTACW 1 cut(s) 2793
TauI GCSGC 1 cut(s) 2488
TfiI GAWTC 6 cut(s) 47, 1120, 1429, 1618, 2352, 2474
Tru1I TTAA 6 cut(s) 392, 921, 1184, 1272, 1398, 1866
Tru9I TTAA 6 cut(s) 392, 921, 1184, 1272, 1398, 1866
TscAI CASTG 1 cut(s) 2593
TseFI GTSAC 5 cut(s) 683, 1433, 1457, 2071, 2366
TseI GCWGC 4 cut(s) 187, 1379, 1508, 2569
Tsp45I GTSAC 5 cut(s) 683, 1433, 1457, 2071, 2366
TspDTI ATGAA 9 cut(s) 215, 270, 537, 1255, 2159, 2165, 2193, 2562, 2670
TspGWI ACGGA 3 cut(s) 366, 440, 1166
TspRI CASTG 1 cut(s) 2593
Van91I CCANNNNNTGG 2 cut(s) 476, 2539
VpaK11BI GGWCC 3 cut(s) 62, 649, 2317
XagI CCTNNNNNAGG 2 cut(s) 1825, 1976
XapI RAATTY 8 cut(s) 291, 485, 1187, 1774, 1894, 1947, 2696, 2744
XbaI TCTAGA 3 cut(s) 1709, 2125, 2663
XceI RCATGY 4 cut(s) 530, 1386, 1684, 1906
XcmI CCANNNNNNNNNTGG 2 cut(s) 677, 1736
XmiI GTMKAC 1 cut(s) 1853
XmnI GAANNNNTTC 1 cut(s) 1922
XspI CTAG 5 cut(s) 515, 1710, 1782, 2126, 2664
Zsp2I ATGCAT 2 cut(s) 1422, 1686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.