Rorug02G0531800

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000363
Physical Location & Seq
Reverse (-)
66165587 .. 66173146
7560 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0531800.1

Sequence Viewer

Length: 2226 bp
ATGGATTTGTTCCAGCCAAACATGATGGAAGGTGGAGGTCATTTCGGAAACCCACTGGACATGATGGCCACTCAGAACACATCAGAAAGCGAGCTGAATGCACGTCTCGGTGATGATTTCGACAGTGCTACAAAATCTGGCAGCGAGAACCTGGAAGGAGGTGGCTCCGGCGACGATGGACAAGACCCTCGCCCCAACAAGAAGAAGAGATATCACCGCCACACGCAGCACCAGATCCAAGAAATGGAAGCTTTCTTCAAGGAGTGTCCACACCCTGATGACAAGCAAAGGAAGGAATTGAGTCGAGAGTTACGTTTGGAACCATTACAAGTTAAATTTTGGTTCCAAAACAAGCGCACCCAGATGAAGACGCAGCATGAGAGGCATGAGAACACGAGCCTTCGTAATGAAAATGAAAAGCTTCGAGCTGATAACATGAGATATCGAGAGGCACTGAGCAATGCATCATGTCCTAATTGCGGAGGCCCAACGGCTATTGGAGAGATGTCCTTCGATGAGCATCATTTGAGACTTGAAAATGCTCGATTAAGAGAAGAGATTGATAGAATATCAGCAATTGCTGCTAAATATGTCGGCAAGCCAGTGGGGAACTACCCTCTTATATCTTCACCAGTTCCTCCTAACCGCAGTCCACTTGATCTTGGAGTGGGAAACATGGGGACCAGTCCTCACCACAGCTTCTTATTAAACTCAGACATGTATGCTCCAGGAGACATTATGCGGTCAATCGGTGCACCTAGTGAGGCAGATAAGCCCATGATTATTGAGCTTGCAGTTGCAGCTATGGAGGAGCTAATCCGAATGGCTCAAATTGGTGAACCTTTGTGGATGAATAGCATTGATGGATCAACTACTGTGCTCAATGAGGATGAGTACATTCGAACATTTCCTCGTGGAATTGTGCCAAAACAATCTGGATTCAAATGTGAAGCTTCGCGGGAGAGTGCTGTTGTCATTATGAACCACAATAACCTTGTTGAGATTCTCATGGATGTGAATCAATGGTCAACTGTATTTTCGGGGATTGTATCAAGAGCTATGACTCTGGATGTGCTATCAACTGGAGTAGCAGGAAACTACAATGGAGCCTTGCAAGTGATGACAGCCGAATTTCAAGTACCATCTCCATTGGTTCCAACTCGTGAGAGTTATTTTGTGAGGTACTGTAAGCAGCATGTCGATGGCACTTGGGCAGTGGTTGATGTCTCCTTGGACAATCAACGCGGCTATACTTCTGCTCTGAAATCATGCCAAAGAAGACCTTCTGGTTGTCTCATCCAAGAAATGCCAAATGGATACTCAAAGGTTACATGGATTGAGCATGTAGAAGTGGATGAAAGAGGTGTTCACAATCTGTACAAGCAGCTGGTCAACTCTGGCAATGCATTCGGGGCAAAAAGATGGGTTGCGACCTTGGACCGCCAATGTGAGCGTCTTGCCAGCGCCTTGGCGACAAACATCCCTACCGGTGATGTTGGTGTGATAACAACTCAAGAAGGGAGGAAGAGCATGTTGAAGCTGGCCGAGAGAATGGTTATAAGTTTCTGTGCTGGAGTGAGTGCATCTACCACTCATACATGGACGACGCTCTCTGGAACAGGGGCTGATGATGTTCGTGTGATGACCCGAAAGAGTGTGGATGATCCAGGCAGGCCTCCAGGCATTGTTCTCAGCGCTGCAACCTCATTCTGGCTTCCTGTCCCCCCCAAAAGGGTCTTTGAGTTCCTTCGTGATGAGAACTCACGAAATGAGTGGGATATCCTCTCCAATGGTGGCGTCGTGCAAGAAATGGCGCACATAGCCAACGGACGTGACACTGGGAACTGCGTGTCACTACTTCGAAGTGCCAATTCAAGTCAGAGCAACATGCTCATACTGCAGGAGAGCTGCACTGACTCAACGGCATCATATGTAATCTATGCCCCTGTGGACATAGTTGCTATGAATGTCGTTCTGAATGGCAGTGACCCTGACTATGTTGCCCTGCTGCCATCTGGGTTTGCTATACTCCCAGATGGAACTAGCGGGAGCAACAACGGGGAAGGTGAATCTGGAGGGTCCTTGTTGACTGTTGCCTTCCAGATCCTGGTGGATTCAGTTCCCACCGCAAAGCTGTCCCTGGGGTCTGTTGCAACAGTCAACAACCTCATAGCTTGCACAGTCGAGCGGATCAAGGCCTCATTGTCCTTGGACAATGAAGCCTGA

Protein Analysis

741

Amino Acids

81.3

Weight (kDa)

5.46

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 68 - 123 1.2e-17 Homeodomain
START PF01852 264 - 485 2.1e-53 START domain
PDF2_C PF25797 504 - 736 7.3e-103 PDF2-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12210 AT1G12210 AT1G12210 AT1G12220 AT1G12220 AT1G12220 AT1G12280 AT1G12280 AT1G12290 AT1G12290 AT1G12290 AT1G12290 AT1G15890 AT1G15890 AT1G51480 AT1G52660 AT1G52660 AT1G61180 AT1G61180 AT1G61190 AT1G61190 AT1G61190 AT1G61190 AT1G61300 AT1G61300 AT1G61310 AT3G15700 AT3G15700 AT4G10780 AT4G10780 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43740 AT5G43740 AT5G63020
fragaria_vesca FvH4_6g30050 FvH4_6g48220
malus_domestica MD09G1055800.v1.1 MD09G1198800.v1.1 MD17G1204800.v1.1 MD17G1204900.v1.1 MD17G1205000.v1.1 MD17G1205300.v1.1 MD17G1205700.v1.1 MD17G1205800.v1.1 MD17G1224100.v1.1
prunus_persica Prupe.3G054800_v2.0.a1 Prupe.3G054800_v2.0.a1 Prupe.3G080300_v2.0.a1 Prupe.3G080400_v2.0.a1 Prupe.3G080500_v2.0.a1 Prupe.3G080600_v2.0.a1 Prupe.3G080800_v2.0.a1 Prupe.3G083200_v2.0.a1 Prupe.3G099800_v2.0.a1 Prupe.3G099800_v2.0.a1
pyrus_communis pycom17g20860 pycom17g20910 pycom17g22790
rosa_chinensis RchiOBHm_Chr2g0136891 RchiOBHm_Chr2g0136901 RchiOBHm_Chr2g0167711 RchiOBHm_Chr2g0167721 RchiOBHm_Chr2g0167731 RchiOBHm_Chr4g0407611
rosa_laevigata RLG00000018943 RLG00000019278 RLG00000019282 RLG00000019587 RLG00000021748 RLG00000021749 RLG00000031057
rosa_multiflora Rmu_sc0002143.1_g000003 Rmu_sc0002782.1_g000001 Rmu_sc0023879.1_g000001 Rmu_sc0042171.1_g000001
rosa_roxburghii Rroxscaffold_2G00083300 Rroxscaffold_2G00107890 Rroxscaffold_2G00116680
rosa_rugosa Rorug02G0271700 Rorug02G0334900 Rorug02G0531800 Rorug02G0531800
rosa_samantha Rh2AG359900 Rh2AG385100 Rh2AG600500 Rh2AG600600 Rh2BG392200 Rh2BG611000 Rh2BG611100 Rh2BG611200 Rh2CG343100 Rh2CG343300 Rh2CG372300 Rh2CG581400 Rh2CG581500 Rh2DG382900 Rh2DG623700 Rh5AG027500 Rh5CG029900
rosa_wichuraiana Rw2G031490 Rw2G049850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1559
AccB7I CCANNNNNTGG 2 cut(s) 244, 2107
AccBSI CCGCTC 1 cut(s) 2188
AccII CGCG 2 cut(s) 958, 1245
AclWI GGATC 5 cut(s) 229, 874, 1658, 2098, 2198
AcoI YGGCCR 2 cut(s) 66, 1542
AcsI RAATTY 2 cut(s) 335, 1130
AcyI GRCGYC 1 cut(s) 1797
AdeI CACNNNGTG 1 cut(s) 761
AfaI GTAC 4 cut(s) 896, 1140, 1184, 1379
AfeI AGCGCT 1 cut(s) 1696
AfiI CCNNNNNNNGG 6 cut(s) 244, 479, 1710, 1731, 1732, 2107
AflIII ACRYGT 1 cut(s) 717
AgeI ACCGGT 1 cut(s) 1487
AgsI TTSAA 6 cut(s) 259, 536, 943, 1136, 1537, 1875
AjiI CACGTC 2 cut(s) 104, 1832
AjnI CCWGG 6 cut(s) 150, 727, 1666, 1678, 2106, 2139
Alw21I GWGCWC 2 cut(s) 757, 882
Alw26I GTCTC 5 cut(s) 110, 523, 726, 1231, 1298
Alw44I GTGCAC 1 cut(s) 753
AlwI GGATC 5 cut(s) 229, 874, 1658, 2098, 2198
AlwNI CAGNNNCTG 2 cut(s) 1625, 2107
Aor51HI AGCGCT 1 cut(s) 1696
AoxI GGCC 5 cut(s) 66, 484, 1542, 1673, 2196
ApaLI GTGCAC 1 cut(s) 753
ApoI RAATTY 2 cut(s) 335, 1130
AsiGI ACCGGT 1 cut(s) 1487
Asp700I GAANNNNTTC 2 cut(s) 420, 1282
AspLEI GCGC 4 cut(s) 357, 1466, 1697, 1816
AspS9I GGNCC 4 cut(s) 485, 681, 1438, 2079
AsuHPI GGTGA 7 cut(s) 122, 206, 621, 683, 848, 1502, 2078
AsuII TTCGAA 2 cut(s) 901, 1861
AvaII GGWCC 3 cut(s) 681, 1438, 2079
BaeGI GKGCMC 1 cut(s) 757
BalI TGGCCA 1 cut(s) 68
BauI CACGAG 3 cut(s) 394, 912, 1161
BbsI GAAGAC 2 cut(s) 374, 1285
Bbv12I GWGCWC 2 cut(s) 757, 882
BccI CCATC 9 cut(s) 19, 58, 170, 857, 1150, 1196, 1416, 2020, 2030
BceAI ACGGC 2 cut(s) 507, 1938
BcgI CGANNNNNNTGC 4 cut(s) 80, 114, 1390, 1424
BciT130I CCWGG 6 cut(s) 152, 729, 1668, 1680, 2108, 2141
BciVI GTATCC 1 cut(s) 1310
BcoDI GTCTC 5 cut(s) 110, 523, 726, 1231, 1298
BfaI CTAG 2 cut(s) 759, 2043
BfmI CTRYAG 1 cut(s) 1898
BfoI RGCGCY 2 cut(s) 1467, 1698
BfuI GTATCC 1 cut(s) 1310
Bme1390I CCNGG 6 cut(s) 152, 729, 1668, 1680, 2108, 2141
Bme18I GGWCC 3 cut(s) 681, 1438, 2079
BmgBI CACGTC 2 cut(s) 104, 1832
BmgT120I GGNCC 4 cut(s) 485, 681, 1438, 2079
BmiI GGNNCC 7 cut(s) 166, 321, 344, 682, 1108, 1155, 2080
BmrFI CCNGG 6 cut(s) 152, 729, 1668, 1680, 2108, 2141
BmrI ACTGGG 1 cut(s) 1848
BmsI GCATC 4 cut(s) 473, 529, 1592, 1934
BmuI ACTGGG 1 cut(s) 1848
BpiI GAAGAC 2 cut(s) 374, 1285
BpmI CTGGAG 5 cut(s) 711, 1104, 1593, 1662, 2094
Bpu14I TTCGAA 2 cut(s) 901, 1861
BpuEI CTTGAG 1 cut(s) 1497
BsaBI GATNNNNATC 2 cut(s) 519, 1017
BsaHI GRCGYC 1 cut(s) 1797
BsaJI CCNNGG 6 cut(s) 1230, 1434, 1467, 2139, 2140, 2208
BsaWI WCCGGW 1 cut(s) 1487
Bsc4I CCNNNNNNNGG 6 cut(s) 244, 479, 1710, 1731, 1732, 2107
Bse118I RCCGGY 1 cut(s) 1487
Bse1I ACTGG 6 cut(s) 60, 602, 632, 684, 1087, 1843
Bse3DI GCAATG 2 cut(s) 466, 1408
Bse8I GATNNNNATC 2 cut(s) 519, 1017
BseBI CCWGG 6 cut(s) 152, 729, 1668, 1680, 2108, 2141
BseDI CCNNGG 6 cut(s) 1230, 1434, 1467, 2139, 2140, 2208
BseGI GGATG 8 cut(s) 855, 895, 1018, 1075, 1296, 1360, 1479, 1666
BseJI GATNNNNATC 2 cut(s) 519, 1017
BseLI CCNNNNNNNGG 6 cut(s) 244, 479, 1710, 1731, 1732, 2107
BseMI GCAATG 2 cut(s) 466, 1408
BseMII CTCAG 4 cut(s) 86, 446, 726, 1705
BseNI ACTGG 6 cut(s) 60, 602, 632, 684, 1087, 1843
BseRI GAGGAG 1 cut(s) 824
BseSI GKGCMC 1 cut(s) 757
BsgI GTGCAG 1 cut(s) 1894
Bsh1236I CGCG 2 cut(s) 958, 1245
BshFI GGCC 5 cut(s) 68, 486, 1544, 1675, 2198
BshTI ACCGGT 1 cut(s) 1487
BsiHKAI GWGCWC 2 cut(s) 757, 882
BsiSI CCGG 2 cut(s) 168, 1488
BslFI GGGAC 3 cut(s) 694, 1706, 2122
BslI CCNNNNNNNGG 6 cut(s) 244, 479, 1710, 1731, 1732, 2107
BsmAI GTCTC 5 cut(s) 110, 523, 726, 1231, 1298
BsmBI CGTCTC 1 cut(s) 110
BsmFI GGGAC 3 cut(s) 694, 1706, 2122
BsmI GAATGC 2 cut(s) 103, 1406
BsnI GGCC 5 cut(s) 68, 486, 1544, 1675, 2198
Bsp119I TTCGAA 2 cut(s) 901, 1861
Bsp1286I GDGCHC 2 cut(s) 757, 882
Bsp1407I TGTACA 1 cut(s) 1377
Bsp143I GATC 6 cut(s) 234, 658, 866, 1663, 2103, 2190
BspANI GGCC 5 cut(s) 68, 486, 1544, 1675, 2198
BspCNI CTCAG 4 cut(s) 85, 447, 725, 1704
BspFNI CGCG 2 cut(s) 958, 1245
BspLI GGNNCC 7 cut(s) 166, 321, 344, 682, 1108, 1155, 2080
BspMAI CTGCAG 1 cut(s) 1902
BspPI GGATC 5 cut(s) 229, 874, 1658, 2098, 2198
BspQI GCTCTTC 1 cut(s) 1520
BspT104I TTCGAA 2 cut(s) 901, 1861
BsrBI CCGCTC 1 cut(s) 2188
BsrDI GCAATG 2 cut(s) 466, 1408
BsrFI RCCGGY 1 cut(s) 1487
BsrGI TGTACA 1 cut(s) 1377
BsrI ACTGG 6 cut(s) 60, 602, 632, 684, 1087, 1843
BssAI RCCGGY 1 cut(s) 1487
BssECI CCNNGG 6 cut(s) 1230, 1434, 1467, 2139, 2140, 2208
BssMI GATC 6 cut(s) 234, 658, 866, 1663, 2103, 2190
BssNI GRCGYC 1 cut(s) 1797
BssSI CACGAG 3 cut(s) 394, 912, 1161
BssT1I CCWWGG 4 cut(s) 1230, 1434, 1467, 2208
Bst2BI CACGAG 3 cut(s) 394, 912, 1161
Bst2UI CCWGG 6 cut(s) 152, 729, 1668, 1680, 2108, 2141
Bst4CI ACNGT 7 cut(s) 125, 877, 1033, 1187, 2092, 2158, 2182
Bst6I CTCTTC 3 cut(s) 200, 549, 1520
BstACI GRCGYC 1 cut(s) 1797
BstAPI GCANNNNNTGC 1 cut(s) 581
BstAUI TGTACA 1 cut(s) 1377
BstBI TTCGAA 2 cut(s) 901, 1861
BstC8I GCNNGC 7 cut(s) 92, 599, 792, 1462, 1542, 1673, 2176
BstDEI CTNAG 4 cut(s) 72, 455, 712, 1691
BstF5I GGATG 8 cut(s) 855, 895, 1018, 1075, 1296, 1360, 1479, 1666
BstFNI CGCG 2 cut(s) 958, 1245
BstH2I RGCGCY 2 cut(s) 1467, 1698
BstHHI GCGC 4 cut(s) 357, 1466, 1697, 1816
BstKTI GATC 6 cut(s) 237, 661, 869, 1666, 2106, 2193
BstMAI GTCTC 5 cut(s) 110, 523, 726, 1231, 1298
BstMBI GATC 6 cut(s) 234, 658, 866, 1663, 2103, 2190
BstMWI GCNNNNNNNGC 6 cut(s) 382, 581, 800, 1412, 1820, 1897
BstNI CCWGG 6 cut(s) 152, 729, 1668, 1680, 2108, 2141
BstNSI RCATGY 5 cut(s) 721, 1199, 1346, 1534, 1891
BstSCI CCNGG 6 cut(s) 150, 727, 1666, 1678, 2106, 2139
BstSFI CTRYAG 1 cut(s) 1898
BstSLI GKGCMC 1 cut(s) 757
BstUI CGCG 2 cut(s) 958, 1245
BstV2I GAAGAC 2 cut(s) 374, 1285
BstX2I RGATCY 2 cut(s) 234, 2103
BstXI CCANNNNNNTGG 1 cut(s) 1468
BstYI RGATCY 2 cut(s) 234, 2103
BsuI GTATCC 1 cut(s) 1310
BsuRI GGCC 5 cut(s) 68, 486, 1544, 1675, 2198
BtrI CACGTC 2 cut(s) 104, 1832
BtsCI GGATG 8 cut(s) 855, 895, 1018, 1075, 1296, 1360, 1479, 1666
BtsI GCAGTG 2 cut(s) 1221, 1990
BtsIMutI CAGTG 8 cut(s) 53, 130, 452, 609, 1221, 1836, 1911, 1990
Cac8I GCNNGC 7 cut(s) 92, 599, 792, 1462, 1542, 1673, 2176
CaiI CAGNNNCTG 2 cut(s) 1625, 2107
CfoI GCGC 4 cut(s) 357, 1466, 1697, 1816
Cfr10I RCCGGY 1 cut(s) 1487
Cfr13I GGNCC 4 cut(s) 485, 681, 1438, 2079
CseI GACGC 4 cut(s) 379, 1442, 1615, 1786
Csp6I GTAC 4 cut(s) 895, 1139, 1183, 1378
CspAI ACCGGT 1 cut(s) 1487
CviQI GTAC 4 cut(s) 895, 1139, 1183, 1378
DdeI CTNAG 4 cut(s) 72, 455, 712, 1691
DpnI GATC 6 cut(s) 236, 660, 868, 1665, 2105, 2192
DpnII GATC 6 cut(s) 234, 658, 866, 1663, 2103, 2190
DraIII CACNNNGTG 1 cut(s) 761
EaeI YGGCCR 2 cut(s) 66, 1542
Eam1104I CTCTTC 3 cut(s) 200, 549, 1520
EarI CTCTTC 3 cut(s) 200, 549, 1520
Eco130I CCWWGG 4 cut(s) 1230, 1434, 1467, 2208
Eco147I AGGCCT 2 cut(s) 1675, 2198
Eco32I GATATC 3 cut(s) 212, 443, 1780
Eco47I GGWCC 3 cut(s) 681, 1438, 2079
Eco47III AGCGCT 1 cut(s) 1696
EcoO109I RGGNCCY 1 cut(s) 2079
EcoRII CCWGG 6 cut(s) 150, 727, 1666, 1678, 2106, 2139
EcoRV GATATC 3 cut(s) 212, 443, 1780
EcoT14I CCWWGG 4 cut(s) 1230, 1434, 1467, 2208
EcoT22I ATGCAT 2 cut(s) 466, 1408
ErhI CCWWGG 4 cut(s) 1230, 1434, 1467, 2208
Esp3I CGTCTC 1 cut(s) 110
FalI AAGNNNNNCTT 2 cut(s) 1267, 1299
FaqI GGGAC 3 cut(s) 694, 1706, 2122
FauI CCCGC 2 cut(s) 951, 2039
FauNDI CATATG 1 cut(s) 1930
FokI GGATG 8 cut(s) 862, 902, 1025, 1082, 1283, 1367, 1466, 1673
FspBI CTAG 2 cut(s) 759, 2043
GlaI GCGC 4 cut(s) 356, 1465, 1696, 1815
GsuI CTGGAG 5 cut(s) 711, 1104, 1593, 1662, 2094
HaeII RGCGCY 2 cut(s) 1467, 1698
HaeIII GGCC 5 cut(s) 68, 486, 1544, 1675, 2198
HapII CCGG 2 cut(s) 168, 1488
HgaI GACGC 4 cut(s) 379, 1442, 1615, 1786
HhaI GCGC 4 cut(s) 357, 1466, 1697, 1816
Hin1I GRCGYC 1 cut(s) 1797
Hin6I GCGC 4 cut(s) 355, 1464, 1695, 1814
HinP1I GCGC 4 cut(s) 355, 1464, 1695, 1814
HincII GTYRAC 4 cut(s) 1029, 1393, 2088, 2161
HindII GTYRAC 4 cut(s) 1029, 1393, 2088, 2161
HindIII AAGCTT 3 cut(s) 249, 419, 951
HinfI GANTC 8 cut(s) 301, 939, 1003, 1018, 1063, 1916, 2069, 2114
HpaII CCGG 2 cut(s) 168, 1488
HphI GGTGA 7 cut(s) 122, 206, 621, 683, 848, 1502, 2078
Hpy188I TCNGA 8 cut(s) 47, 75, 85, 715, 821, 1263, 1881, 1977
Hpy99I CGWCG 3 cut(s) 176, 1609, 1802
HpyCH4III ACNGT 7 cut(s) 125, 877, 1033, 1187, 2092, 2158, 2182
HpyCH4IV ACGT 3 cut(s) 103, 313, 1831
HpyF10VI GCNNNNNNNGC 6 cut(s) 382, 581, 800, 1412, 1820, 1897
HpyF3I CTNAG 4 cut(s) 72, 455, 712, 1691
HpySE526I ACGT 3 cut(s) 103, 313, 1831
Hsp92I GRCGYC 1 cut(s) 1797
HspAI GCGC 4 cut(s) 355, 1464, 1695, 1814
Kzo9I GATC 6 cut(s) 234, 658, 866, 1663, 2103, 2190
LguI GCTCTTC 1 cut(s) 1520
LmnI GCTCC 5 cut(s) 170, 730, 811, 1106, 2049
LweI GCATC 4 cut(s) 473, 529, 1592, 1934
MaeI CTAG 2 cut(s) 759, 2043
MaeII ACGT 3 cut(s) 103, 313, 1831
MaeIII GTNAC 5 cut(s) 309, 1327, 1832, 1851, 1985
MalI GATC 6 cut(s) 236, 660, 868, 1665, 2105, 2192
MbiI CCGCTC 1 cut(s) 2188
MboI GATC 6 cut(s) 234, 658, 866, 1663, 2103, 2190
MboII GAAGA 8 cut(s) 214, 217, 247, 379, 566, 618, 1290, 1537
MfeI CAATTG 1 cut(s) 576
MflI RGATCY 2 cut(s) 234, 2103
MhlI GDGCHC 2 cut(s) 757, 882
MlsI TGGCCA 1 cut(s) 68
MluCI AATT 8 cut(s) 296, 335, 475, 576, 831, 918, 1130, 1870
MluNI TGGCCA 1 cut(s) 68
MlyI GAGTC 3 cut(s) 310, 1057, 1910
MmeI TCCRAC 1 cut(s) 1181
Mox20I TGGCCA 1 cut(s) 68
Mph1103I ATGCAT 2 cut(s) 466, 1408
MroXI GAANNNNTTC 2 cut(s) 420, 1282
MscI TGGCCA 1 cut(s) 68
MseI TTAA 3 cut(s) 333, 548, 707
MslI CAYNNNNRTG 4 cut(s) 276, 362, 1013, 1200
Msp20I TGGCCA 1 cut(s) 68
MspA1I CMGCKG 1 cut(s) 1387
MspI CCGG 2 cut(s) 168, 1488
MspR9I CCNGG 6 cut(s) 152, 729, 1668, 1680, 2108, 2141
MunI CAATTG 1 cut(s) 576
Mva1269I GAATGC 2 cut(s) 103, 1406
MvaI CCWGG 6 cut(s) 152, 729, 1668, 1680, 2108, 2141
MvnI CGCG 2 cut(s) 958, 1245
MwoI GCNNNNNNNGC 6 cut(s) 382, 581, 800, 1412, 1820, 1897
NdeI CATATG 1 cut(s) 1930
NdeII GATC 6 cut(s) 234, 658, 866, 1663, 2103, 2190
NlaIV GGNNCC 7 cut(s) 166, 321, 344, 682, 1108, 1155, 2080
NmeAIII GCCGAG 1 cut(s) 1570
NmuCI GTSAC 3 cut(s) 1832, 1851, 1985
NsiI ATGCAT 2 cut(s) 466, 1408
NspI RCATGY 5 cut(s) 721, 1199, 1346, 1534, 1891
NspV TTCGAA 2 cut(s) 901, 1861
PasI CCCWGGG 1 cut(s) 2140
PceI AGGCCT 2 cut(s) 1675, 2198
PciI ACATGT 1 cut(s) 717
PciSI GCTCTTC 1 cut(s) 1520
PcsI WCGNNNNNNNCGW 1 cut(s) 310
PctI GAATGC 2 cut(s) 103, 1406
PdmI GAANNNNTTC 2 cut(s) 420, 1282
PfeI GAWTC 5 cut(s) 939, 1003, 1018, 2069, 2114
PflMI CCANNNNNTGG 2 cut(s) 244, 2107
PfoI TCCNGGA 1 cut(s) 727
PinAI ACCGGT 1 cut(s) 1487
PleI GAGTC 3 cut(s) 309, 1057, 1910
PpsI GAGTC 3 cut(s) 309, 1057, 1910
PpuMI RGGWCCY 1 cut(s) 2079
PscI ACATGT 1 cut(s) 717
PsiI TTATAA 1 cut(s) 1559
Psp5II RGGWCCY 1 cut(s) 2079
Psp6I CCWGG 6 cut(s) 150, 727, 1666, 1678, 2106, 2139
PspGI CCWGG 6 cut(s) 150, 727, 1666, 1678, 2106, 2139
PspN4I GGNNCC 7 cut(s) 166, 321, 344, 682, 1108, 1155, 2080
PspPI GGNCC 4 cut(s) 485, 681, 1438, 2079
PspPPI RGGWCCY 1 cut(s) 2079
PstI CTGCAG 1 cut(s) 1902
PstNI CAGNNNCTG 2 cut(s) 1625, 2107
PsuI RGATCY 2 cut(s) 234, 2103
PvuII CAGCTG 1 cut(s) 1387
RsaI GTAC 4 cut(s) 896, 1140, 1184, 1379
RsaNI GTAC 4 cut(s) 895, 1139, 1183, 1378
RseI CAYNNNNRTG 4 cut(s) 276, 362, 1013, 1200
SapI GCTCTTC 1 cut(s) 1520
SaqAI TTAA 3 cut(s) 333, 548, 707
Sau3AI GATC 6 cut(s) 234, 658, 866, 1663, 2103, 2190
Sau96I GGNCC 4 cut(s) 485, 681, 1438, 2079
SchI GAGTC 3 cut(s) 310, 1057, 1910
ScrFI CCNGG 6 cut(s) 152, 729, 1668, 1680, 2108, 2141
SduI GDGCHC 2 cut(s) 757, 882
SfaNI GCATC 4 cut(s) 473, 529, 1592, 1934
SfcI CTRYAG 1 cut(s) 1898
SfuI TTCGAA 2 cut(s) 901, 1861
SinI GGWCC 3 cut(s) 681, 1438, 2079
SmiMI CAYNNNNRTG 4 cut(s) 276, 362, 1013, 1200
SmlI CTYRAG 1 cut(s) 1512
SmoI CTYRAG 1 cut(s) 1512
Sse9I AATT 8 cut(s) 296, 335, 475, 576, 831, 918, 1130, 1870
SseBI AGGCCT 2 cut(s) 1675, 2198
SspMI CTAG 2 cut(s) 759, 2043
StuI AGGCCT 2 cut(s) 1675, 2198
StyD4I CCNGG 6 cut(s) 150, 727, 1666, 1678, 2106, 2139
StyI CCWWGG 4 cut(s) 1230, 1434, 1467, 2208
TaaI ACNGT 7 cut(s) 125, 877, 1033, 1187, 2092, 2158, 2182
TaiI ACGT 3 cut(s) 106, 316, 1834
TasI AATT 8 cut(s) 296, 335, 475, 576, 831, 918, 1130, 1870
TatI WGTACW 2 cut(s) 894, 1377
TauI GCSGC 1 cut(s) 1248
TfiI GAWTC 5 cut(s) 939, 1003, 1018, 2069, 2114
Tru1I TTAA 3 cut(s) 333, 548, 707
Tru9I TTAA 3 cut(s) 333, 548, 707
TscAI CASTG 8 cut(s) 60, 130, 459, 609, 1221, 1843, 1918, 1990
TseFI GTSAC 3 cut(s) 1832, 1851, 1985
Tsp45I GTSAC 3 cut(s) 1832, 1851, 1985
TspDTI ATGAA 7 cut(s) 380, 423, 429, 866, 995, 1371, 1979
TspGWI ACGGA 1 cut(s) 1842
TspRI CASTG 8 cut(s) 60, 130, 459, 609, 1221, 1843, 1918, 1990
Van91I CCANNNNNTGG 2 cut(s) 244, 2107
VneI GTGCAC 1 cut(s) 753
VpaK11BI GGWCC 3 cut(s) 681, 1438, 2079
XapI RAATTY 2 cut(s) 335, 1130
XceI RCATGY 5 cut(s) 721, 1199, 1346, 1534, 1891
XmnI GAANNNNTTC 2 cut(s) 420, 1282
XspI CTAG 2 cut(s) 759, 2043
Zsp2I ATGCAT 2 cut(s) 466, 1408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.