Rh2AG600500

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000363
Physical Location & Seq
Reverse (-)
82957936 .. 82959489
1554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG600500.1

Sequence Viewer

Length: 1554 bp
ATGGGAGACCAGTTGTTCCCTCTCTTGAAATTCAGTTACGATAATTTACCTAGTGAGAAAGTCAGATCATGCTTCTTGTACTGTGCTTTATTTCCTGAAGACTATACCATACCTAAAGATGATTTAGCATGCTTTTGGATGTGTGAAAATATGTTAGATGAACATACTGATCTAGAAGAAGCACGAGATGAGAGCTACCATATCATAGGTACTCTTCTCAATGCATGTATGCAAGAAGAGAGTAAAGAAGGTTGTGTAAAAATGCATGATGTTGTTCGTGACATGGCATTGTGGTTGGCTTGTGATCCTAACAAAGCAGAAGAGAGTTTTCTTGTGCGTGCAGGCGCTGATTTGACTGAAGCACCAATTGCCGAAAAATGGAAGAACTCGAAAAGGGTATCATTGATGGCTAACCACATCAAAGAATTGGTTGAAAAACCAGACTCTCCATATCTGTTGACTTTGTTTCTCAGGAGTAATCATCTGAAAATGATTATTACAGGCTTCTTTGACTCTATGTCTAATCTGCTGGTTTTAGATCTGTCTCGCAATATGGATCTAACCCAACTGCCGGTAGGAGTTTCAAGCTTGGTTTCACTACAACATCTCAATTTGTCATACACTGGTATAAGAGAGTTGCCGATTGAGTTAAAGTGCTTAAAGAGGCTGACATATTTGAATTTGGAGTATACACTGAAACTTGATTCTCTTCCACCAACCATACTATCAAGTTTTTCGATGCTAAAAGCTCTGAGAATGGTACATTCTGGTACTTCTGATGATCGAAATCATTTTGATGATGAAAAAGTGATGGTAGAGGAATTGCATGGTTTGAAACACCTGGACTACTTGACTCTGGACATTAGAAGTACCTCTTGTTTCCAAAACTTCGTCAGCAACAAATTAGCGAAGTGCTGCACTCGAGCTCTACACCTCATGGGTTATGACAATCATTCGAGCTCTCTTGATATATCATCTGCGGAGACAAAACATCTTGAGCAGCTTGAGATTAGCGGCTATCCTAATATGGAAGATATAATAGTGGACATTGATTGGGCAGGAGAACGAGCATGTAACAATCCTCGGAACTCAATGATGAGAATCCAGAGCTGCTTCCTTGGCCTTCAATACATAATGGTAAACGCATGTGCAGATCTCAAGGACTTGACACAGCTCTGTTTTGTTCCAAATCTCCAATTTGTTATAGTGTTAAGATGCTCTAGAATGGAGACAATAATCAATTTGAGTAAACTGGGTGGAGATGCAAATGTGGCAAAAGAATTTAACCTGTTTGCGAAACTGAAGCGTCTTGATTTGGCATTTCTACCAGCGTTGGAGAGCGTATACAAGACTGCCTTGCCCTTTCCATGTCTAAAGTGGTTCAGCGTATATCAATGTCCAGCACTGAAGAAACTGCCACTCAACTCTAGCACTGCTCAAGGAGACGGATGCAATTTCATCCTAAGAGGAGAGCAGAAGTGGTGGGATGGGTTAGAGTGGGAGGACCAACTTGCACTAGATACTATTCGTCCCTGCTACATGCGTTGGAATTGA

Protein Analysis

517

Amino Acids

59.15

Weight (kDa)

5.35

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 30 - 96 2.6e-08 Disease resistance protein Winged helix domain
LRR_8 PF13855 152 - 209 1.6e-09 Leucine rich repeat
LRR_14 PF23598 173 - 340 3.1e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 367 - 472 3.8e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12210 AT1G12210 AT1G12210 AT1G12220 AT1G12220 AT1G12220 AT1G12280 AT1G12280 AT1G12290 AT1G12290 AT1G12290 AT1G12290 AT1G15890 AT1G15890 AT1G51480 AT1G52660 AT1G52660 AT1G61180 AT1G61180 AT1G61190 AT1G61190 AT1G61190 AT1G61190 AT1G61300 AT1G61300 AT1G61310 AT3G15700 AT3G15700 AT4G10780 AT4G10780 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43740 AT5G43740 AT5G63020
fragaria_vesca FvH4_6g30050 FvH4_6g48220
malus_domestica MD09G1055800.v1.1 MD09G1198800.v1.1 MD17G1204800.v1.1 MD17G1204900.v1.1 MD17G1205000.v1.1 MD17G1205300.v1.1 MD17G1205700.v1.1 MD17G1205800.v1.1 MD17G1224100.v1.1
prunus_persica Prupe.3G054800_v2.0.a1 Prupe.3G054800_v2.0.a1 Prupe.3G080300_v2.0.a1 Prupe.3G080400_v2.0.a1 Prupe.3G080500_v2.0.a1 Prupe.3G080600_v2.0.a1 Prupe.3G080800_v2.0.a1 Prupe.3G083200_v2.0.a1 Prupe.3G099800_v2.0.a1 Prupe.3G099800_v2.0.a1
pyrus_communis pycom17g20860 pycom17g20910 pycom17g22790
rosa_chinensis RchiOBHm_Chr2g0136891 RchiOBHm_Chr2g0136901 RchiOBHm_Chr2g0167711 RchiOBHm_Chr2g0167721 RchiOBHm_Chr2g0167731 RchiOBHm_Chr4g0407611
rosa_laevigata RLG00000018943 RLG00000019278 RLG00000019282 RLG00000019587 RLG00000021748 RLG00000021749 RLG00000031057
rosa_multiflora Rmu_sc0002143.1_g000003 Rmu_sc0002782.1_g000001 Rmu_sc0023879.1_g000001 Rmu_sc0042171.1_g000001
rosa_roxburghii Rroxscaffold_2G00083300 Rroxscaffold_2G00107890 Rroxscaffold_2G00116680
rosa_rugosa Rorug02G0271700 Rorug02G0334900 Rorug02G0531800 Rorug02G0531800
rosa_samantha Rh2AG359900 Rh2AG385100 Rh2AG600500 Rh2AG600600 Rh2BG392200 Rh2BG611000 Rh2BG611100 Rh2BG611200 Rh2CG343100 Rh2CG343300 Rh2CG372300 Rh2CG581400 Rh2CG581500 Rh2DG382900 Rh2DG623700 Rh5AG027500 Rh5CG029900
rosa_wichuraiana Rw2G031490 Rw2G049850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 689, 1344
AciI CCGC 2 cut(s) 980, 1014
AclWI GGATC 2 cut(s) 299, 564
AcsI RAATTY 3 cut(s) 29, 679, 1280
AcuI CTGAAG 4 cut(s) 117, 378, 1322, 1427
AfaI GTAC 5 cut(s) 80, 211, 762, 772, 871
AfiI CCNNNNNNNGG 2 cut(s) 378, 571
AgsI TTSAA 6 cut(s) 28, 434, 585, 679, 835, 1127
AhdI GACNNNNNGTC 1 cut(s) 517
AjnI CCWGG 1 cut(s) 840
AluBI AGCT 8 cut(s) 195, 588, 749, 926, 960, 1003, 1110, 1174
AluI AGCT 8 cut(s) 195, 588, 749, 926, 960, 1003, 1110, 1174
Alw21I GWGCWC 2 cut(s) 928, 962
Alw26I GTCTC 4 cut(s) 549, 977, 1223, 1437
AlwI GGATC 2 cut(s) 299, 564
AlwNI CAGNNNCTG 1 cut(s) 347
Ama87I CYCGRG 1 cut(s) 921
AoxI GGCC 1 cut(s) 1120
ApeKI GCWGC 3 cut(s) 915, 1000, 1110
ApoI RAATTY 3 cut(s) 29, 679, 1280
AspLEI GCGC 1 cut(s) 347
AspS9I GGNCC 1 cut(s) 1504
AvaI CYCGRG 1 cut(s) 921
AvaII GGWCC 1 cut(s) 1504
BanII GRGCYC 2 cut(s) 928, 962
BauI CACGAG 1 cut(s) 183
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 2 cut(s) 928, 962
BbvI GCAGC 3 cut(s) 902, 1012, 1097
BccI CCATC 3 cut(s) 400, 805, 1481
BciT130I CCWGG 1 cut(s) 842
BcoDI GTCTC 4 cut(s) 549, 977, 1223, 1437
BfaI CTAG 5 cut(s) 51, 173, 1221, 1428, 1517
BfoI RGCGCY 1 cut(s) 348
BglII AGATCT 2 cut(s) 538, 1153
BisI GCNGC 4 cut(s) 916, 1001, 1015, 1111
BlsI GCNGC 4 cut(s) 917, 1002, 1016, 1112
Bme1390I CCNGG 1 cut(s) 842
Bme18I GGWCC 1 cut(s) 1504
BmeRI GACNNNNNGTC 1 cut(s) 517
BmeT110I CYCGRG 1 cut(s) 921
BmgT120I GGNCC 1 cut(s) 1504
BmrFI CCNGG 1 cut(s) 842
BmrI ACTGGG 1 cut(s) 1262
BmsI GCATC 4 cut(s) 729, 1205, 1252, 1439
BmuI ACTGGG 1 cut(s) 1262
BpiI GAAGAC 1 cut(s) 105
BpuEI CTTGAG 4 cut(s) 1016, 1025, 1142, 1422
BsaBI GATNNNNATC 2 cut(s) 786, 1100
BsaJI CCNNGG 2 cut(s) 1082, 1117
Bsc4I CCNNNNNNNGG 2 cut(s) 378, 571
Bse118I RCCGGY 1 cut(s) 571
Bse1I ACTGG 3 cut(s) 10, 628, 1257
Bse8I GATNNNNATC 2 cut(s) 786, 1100
BseBI CCWGG 1 cut(s) 842
BseDI CCNNGG 2 cut(s) 1082, 1117
BseGI GGATG 4 cut(s) 144, 1454, 1458, 1492
BseJI GATNNNNATC 2 cut(s) 786, 1100
BseLI CCNNNNNNNGG 2 cut(s) 378, 571
BseMII CTCAG 2 cut(s) 484, 743
BseNI ACTGG 3 cut(s) 10, 628, 1257
BseRI GAGGAG 1 cut(s) 1482
BseXI GCAGC 3 cut(s) 902, 1012, 1097
BsgI GTGCAG 3 cut(s) 360, 901, 1170
BshFI GGCC 1 cut(s) 1122
BsiHKAI GWGCWC 2 cut(s) 928, 962
BsiHKCI CYCGRG 1 cut(s) 921
BsiSI CCGG 1 cut(s) 572
BslFI GGGAC 1 cut(s) 1515
BslI CCNNNNNNNGG 2 cut(s) 378, 571
BsmAI GTCTC 4 cut(s) 549, 977, 1223, 1437
BsmBI CGTCTC 1 cut(s) 1437
BsmFI GGGAC 1 cut(s) 1515
BsnI GGCC 1 cut(s) 1122
BsoBI CYCGRG 1 cut(s) 921
Bsp1286I GDGCHC 2 cut(s) 928, 962
Bsp143I GATC 7 cut(s) 65, 169, 304, 538, 556, 781, 1153
BspACI CCGC 2 cut(s) 980, 1014
BspANI GGCC 1 cut(s) 1122
BspCNI CTCAG 2 cut(s) 483, 744
BspPI GGATC 2 cut(s) 299, 564
BsrFI RCCGGY 1 cut(s) 571
BsrI ACTGG 3 cut(s) 10, 628, 1257
BssAI RCCGGY 1 cut(s) 571
BssECI CCNNGG 2 cut(s) 1082, 1117
BssMI GATC 7 cut(s) 65, 169, 304, 538, 556, 781, 1153
BssNAI GTATAC 2 cut(s) 690, 1345
BssSI CACGAG 1 cut(s) 183
BssT1I CCWWGG 1 cut(s) 1117
Bst1107I GTATAC 2 cut(s) 690, 1345
Bst2BI CACGAG 1 cut(s) 183
Bst2UI CCWGG 1 cut(s) 842
Bst4CI ACNGT 1 cut(s) 83
Bst6I CTCTTC 4 cut(s) 219, 231, 315, 714
BstAPI GCANNNNNTGC 1 cut(s) 368
BstC8I GCNNGC 3 cut(s) 130, 339, 343
BstDEI CTNAG 3 cut(s) 470, 752, 1463
BstF5I GGATG 4 cut(s) 144, 1454, 1458, 1492
BstH2I RGCGCY 1 cut(s) 348
BstHHI GCGC 1 cut(s) 347
BstKTI GATC 7 cut(s) 68, 172, 307, 541, 559, 784, 1156
BstMAI GTCTC 4 cut(s) 549, 977, 1223, 1437
BstMBI GATC 7 cut(s) 65, 169, 304, 538, 556, 781, 1153
BstMWI GCNNNNNNNGC 3 cut(s) 368, 1119, 1271
BstNI CCWGG 1 cut(s) 842
BstNSI RCATGY 5 cut(s) 132, 228, 1074, 1149, 1543
BstSCI CCNGG 1 cut(s) 840
BstV1I GCAGC 3 cut(s) 902, 1012, 1097
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 3 cut(s) 538, 556, 1153
BstYI RGATCY 3 cut(s) 538, 556, 1153
BstZ17I GTATAC 2 cut(s) 690, 1345
BsuRI GGCC 1 cut(s) 1122
BtsCI GGATG 4 cut(s) 144, 1454, 1458, 1492
BtsI GCAGTG 1 cut(s) 1431
BtsIMutI CAGTG 4 cut(s) 621, 692, 1403, 1431
Cac8I GCNNGC 3 cut(s) 130, 339, 343
CaiI CAGNNNCTG 1 cut(s) 347
CfoI GCGC 1 cut(s) 347
Cfr10I RCCGGY 1 cut(s) 571
Cfr13I GGNCC 1 cut(s) 1504
CseI GACGC 1 cut(s) 1295
Csp6I GTAC 5 cut(s) 79, 210, 761, 771, 870
CviQI GTAC 5 cut(s) 79, 210, 761, 771, 870
DdeI CTNAG 3 cut(s) 470, 752, 1463
DpnI GATC 7 cut(s) 67, 171, 306, 540, 558, 783, 1155
DpnII GATC 7 cut(s) 65, 169, 304, 538, 556, 781, 1153
DriI GACNNNNNGTC 1 cut(s) 517
Eam1104I CTCTTC 4 cut(s) 219, 231, 315, 714
Eam1105I GACNNNNNGTC 1 cut(s) 517
EarI CTCTTC 4 cut(s) 219, 231, 315, 714
Ecl136II GAGCTC 2 cut(s) 926, 960
Eco130I CCWWGG 1 cut(s) 1117
Eco24I GRGCYC 2 cut(s) 928, 962
Eco47I GGWCC 1 cut(s) 1504
Eco53kI GAGCTC 2 cut(s) 926, 960
Eco57I CTGAAG 4 cut(s) 117, 378, 1322, 1427
Eco88I CYCGRG 1 cut(s) 921
EcoICRI GAGCTC 2 cut(s) 926, 960
EcoRII CCWGG 1 cut(s) 840
EcoT14I CCWWGG 1 cut(s) 1117
EcoT22I ATGCAT 2 cut(s) 226, 267
EcoT38I GRGCYC 2 cut(s) 928, 962
ErhI CCWWGG 1 cut(s) 1117
Esp3I CGTCTC 1 cut(s) 1437
FalI AAGNNNNNCTT 4 cut(s) 859, 891, 1340, 1372
FaqI GGGAC 1 cut(s) 1515
FblI GTMKAC 2 cut(s) 689, 1344
Fnu4HI GCNGC 4 cut(s) 916, 1001, 1015, 1111
FokI GGATG 4 cut(s) 151, 1445, 1461, 1499
FriOI GRGCYC 2 cut(s) 928, 962
Fsp4HI GCNGC 4 cut(s) 916, 1001, 1015, 1111
FspBI CTAG 5 cut(s) 51, 173, 1221, 1428, 1517
GlaI GCGC 1 cut(s) 346
GluI GCNGC 4 cut(s) 916, 1001, 1015, 1111
HaeII RGCGCY 1 cut(s) 348
HaeIII GGCC 1 cut(s) 1122
HapII CCGG 1 cut(s) 572
HgaI GACGC 1 cut(s) 1295
HhaI GCGC 1 cut(s) 347
Hin6I GCGC 1 cut(s) 345
HinP1I GCGC 1 cut(s) 345
HincII GTYRAC 1 cut(s) 459
HindII GTYRAC 1 cut(s) 459
HindIII AAGCTT 1 cut(s) 586
HinfI GANTC 5 cut(s) 443, 512, 704, 853, 1101
HpaII CCGG 1 cut(s) 572
Hpy166II GTNNAC 6 cut(s) 459, 690, 1045, 1141, 1250, 1345
Hpy188I TCNGA 5 cut(s) 65, 486, 753, 778, 1086
Hpy8I GTNNAC 6 cut(s) 459, 690, 1045, 1141, 1250, 1345
HpyAV CCTTC 2 cut(s) 242, 1133
HpyCH4III ACNGT 1 cut(s) 83
HpyF10VI GCNNNNNNNGC 3 cut(s) 368, 1119, 1271
HpyF3I CTNAG 3 cut(s) 470, 752, 1463
HspAI GCGC 1 cut(s) 345
Kzo9I GATC 7 cut(s) 65, 169, 304, 538, 556, 781, 1153
Lsp1109I GCAGC 3 cut(s) 902, 1012, 1097
LweI GCATC 4 cut(s) 729, 1205, 1252, 1439
MaeI CTAG 5 cut(s) 51, 173, 1221, 1428, 1517
MaeIII GTNAC 3 cut(s) 35, 278, 1073
MalI GATC 7 cut(s) 67, 171, 306, 540, 558, 783, 1155
MboI GATC 7 cut(s) 65, 169, 304, 538, 556, 781, 1153
MboII GAAGA 9 cut(s) 110, 188, 206, 248, 332, 394, 701, 1043, 1420
MfeI CAATTG 1 cut(s) 366
MflI RGATCY 3 cut(s) 538, 556, 1153
MhlI GDGCHC 2 cut(s) 928, 962
MlyI GAGTC 3 cut(s) 437, 506, 847
MmeI TCCRAC 2 cut(s) 1314, 1526
MnlI CCTC 8 cut(s) 30, 657, 811, 883, 944, 1092, 1460, 1495
Mph1103I ATGCAT 2 cut(s) 226, 267
MseI TTAA 4 cut(s) 650, 659, 1211, 1284
MslI CAYNNNNRTG 1 cut(s) 795
MspI CCGG 1 cut(s) 572
MspR9I CCNGG 1 cut(s) 842
MunI CAATTG 1 cut(s) 366
MvaI CCWGG 1 cut(s) 842
MwoI GCNNNNNNNGC 3 cut(s) 368, 1119, 1271
NdeII GATC 7 cut(s) 65, 169, 304, 538, 556, 781, 1153
NmuCI GTSAC 1 cut(s) 278
NsiI ATGCAT 2 cut(s) 226, 267
NspI RCATGY 5 cut(s) 132, 228, 1074, 1149, 1543
PaeI GCATGC 1 cut(s) 132
PaeR7I CTCGAG 1 cut(s) 921
PfeI GAWTC 2 cut(s) 704, 1101
PkrI GCNGC 4 cut(s) 917, 1002, 1016, 1112
PleI GAGTC 3 cut(s) 437, 506, 847
PpsI GAGTC 3 cut(s) 437, 506, 847
Psp124BI GAGCTC 2 cut(s) 928, 962
Psp6I CCWGG 1 cut(s) 840
PspGI CCWGG 1 cut(s) 840
PspPI GGNCC 1 cut(s) 1504
PspXI VCTCGAGB 1 cut(s) 921
PstNI CAGNNNCTG 1 cut(s) 347
PsuI RGATCY 3 cut(s) 538, 556, 1153
RsaI GTAC 5 cut(s) 80, 211, 762, 772, 871
RsaNI GTAC 5 cut(s) 79, 210, 761, 771, 870
RseI CAYNNNNRTG 1 cut(s) 795
SacI GAGCTC 2 cut(s) 928, 962
SaqAI TTAA 4 cut(s) 650, 659, 1211, 1284
SatI GCNGC 4 cut(s) 916, 1001, 1015, 1111
Sau3AI GATC 7 cut(s) 65, 169, 304, 538, 556, 781, 1153
Sau96I GGNCC 1 cut(s) 1504
SchI GAGTC 3 cut(s) 437, 506, 847
ScrFI CCNGG 1 cut(s) 842
SduI GDGCHC 2 cut(s) 928, 962
SfaNI GCATC 4 cut(s) 729, 1205, 1252, 1439
Sfr274I CTCGAG 1 cut(s) 921
SinI GGWCC 1 cut(s) 1504
SlaI CTCGAG 1 cut(s) 921
SmiMI CAYNNNNRTG 1 cut(s) 795
SmlI CTYRAG 5 cut(s) 921, 995, 1004, 1157, 1437
SmoI CTYRAG 5 cut(s) 921, 995, 1004, 1157, 1437
SphI GCATGC 1 cut(s) 132
SsiI CCGC 2 cut(s) 980, 1014
SspMI CTAG 5 cut(s) 51, 173, 1221, 1428, 1517
SstI GAGCTC 2 cut(s) 928, 962
StyD4I CCNGG 1 cut(s) 840
StyI CCWWGG 1 cut(s) 1117
TaaI ACNGT 1 cut(s) 83
TaqI TCGA 5 cut(s) 389, 737, 784, 922, 956
TatI WGTACW 1 cut(s) 78
TauI GCSGC 1 cut(s) 1017
TfiI GAWTC 2 cut(s) 704, 1101
Tru1I TTAA 4 cut(s) 650, 659, 1211, 1284
Tru9I TTAA 4 cut(s) 650, 659, 1211, 1284
TscAI CASTG 4 cut(s) 628, 699, 1410, 1438
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 3 cut(s) 915, 1000, 1110
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 3 cut(s) 174, 816, 1447
TspGWI ACGGA 1 cut(s) 1461
TspRI CASTG 4 cut(s) 628, 699, 1410, 1438
VpaK11BI GGWCC 1 cut(s) 1504
XapI RAATTY 3 cut(s) 29, 679, 1280
XbaI TCTAGA 2 cut(s) 172, 1220
XceI RCATGY 5 cut(s) 132, 228, 1074, 1149, 1543
XcmI CCANNNNNNNNNTGG 1 cut(s) 1374
XhoI CTCGAG 1 cut(s) 921
XmiI GTMKAC 2 cut(s) 689, 1344
XspI CTAG 5 cut(s) 51, 173, 1221, 1428, 1517
Zsp2I ATGCAT 2 cut(s) 226, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.