Rh2BG392200

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000363
Physical Location & Seq
Forward (+)
55609967 .. 55613462
3496 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG392200.1

Sequence Viewer

Length: 2607 bp
ATGGCTGGCATCTTGTCAATCAATTTGTCGTCCGATGCCTTCGTTTCTCGCTGCTGGGATTCCCTCGTTGGAAGACAACATTATGTCAGCAAGCTCCAGAAAAACCTTGTAGGTTTGACTACGTCTTTAGAAGAACTGAAGTGTTTAAAGAATGATGTAAAGAGAAAGGTTGAGGTTTCTGAGCAGCAGCCACACATGAAGCGGCTAGAACAGGTACAGATCTGGATTGTGAAAGTGGAAACCATGGAGACTCAAGTCCACGAAGTTCTCAATGCTAGAATTCAAGAAACTGAGAAACTGTGCTGTGGAGGTTGCTGTTCAAAGAACTATATTTCCAGCTACAGGTATGGGAAAAAAGTGGTCAAGAAGTTGCTAGAAATCACTGCTTTAAAGGAGGCCGGAGTTTTTCAAGAGGTAGCCGAGAGGTTACCTGCGCCCTTAGTCAGTCAAAGACCTATTGAGCCAATTGTGGGCATGGAATCCATGTTTGATAAGGTTTGGAGCCACATCGAAGATGAAGAGGTGGGAGTGATTGGCTTGTATGGAATGGGAGGGGTGGGGAAGACCACCCTTCTTACCAACATCAATAACAACTTCCTCCACAGCACTAATGATTTCAATCTTGTGATCTGGATCGTGGTCTCCAAGGACTTCAAGCTTGACAACATTCAAAACAAGATTGGTGAGAAAATTGGGTTTTCGGATGAGACATGGAAGCGTCAAGGGCAGGATGAGAAAGCTGAGGACATCTCCATGATCCTAGGCACAAAGAAGTTCGTCTTGTTCTTGGATGACCTGTGGGAGCGAGTTGAAATCACCAAAATTGGAGTTCCATTTCCTGATAAACGCAACAAGTGCAAAGTACTCTTCACCACGCGTTCTGAGGACGTTTGCGGTCTTATGGATGCTCATGTGAAGATTAAAGTGGAGTGTTTGGCATCGGAAAAAGCATGGACCTTGTTTCAACAGAAAGTTGGAAAAGAAGCTCTTCTAGTTCATCAAGATATCCCTAGACTAGCTGAGATTGTGGCAAAAGAGTGTGGCGGCTTGCCACTTGCATTAATTACGGTTGGTCGGGCCATGGCTTGCAAGAAGACACCTGAAGAATGGGATCATGCAATTCAAGTTCTCAAGAAATCCGCATCAGAGTTCTCTGAAGATTTTCTAATACCTAAAGATACTTTATTAAACTGTTGGATGGCTGAGGAAATGTGGGATGAACATGCTAATGTACATGAAGGACGAAGTGAAAGTTACCATATTATAGGTGTTCTTCTTAATGTATGTTTGTTGGAAGAATATAAAGGAAACTTTGTGAAAATGCATGATGTCATCCGCGACATGGCGTTGTGGTTGGCTTGTGATCCTGCCAAAACAAACGAGGGTTTCCTATTGCATGCAGGTGCAGATTTGATTGAAGCACCCAATGTTGGAAAATGGAAGAGCATGAAAAGAATATCTTTGATGTCTAATCGCATGGAGCATCTAGATGAAACACCGAATTCTCCTAGTTTGTTGACCTTGTTTCTTGGAGGAAATAATTTGAAGAAGATTGATGATGGCTTTTTCAATCACATGCCTGGTTTAAAAGTTTTGGATTTGTCAGACAATAAGGATCTAACGCGGTTGCCATGTGGAGTTTCAAACCTTGTTTCATTGCAGCATCTGAATTTGTCAGAAACTGGTATAAAAGAGTTGTCAGAGGAGATAAAGAACTTATCAAAGCTAAAATATTTGAACTTGGAGTTTACGGGGCAACTTGATTCTGTTCCACCAAATTTTATATCAAGTTTTCCCATGCTGAAAGTTTTGAGAATGTTCAAATGTGGTTCTTCTGACAAAATTCTTTTTGAAGGTGAAATATCCATGATAGGGGAATTGCAGGGTTTGAAACACTTTGAAGTGTTGACTTTGAGCATTCGAAGTACAGCTTGCTTTTGCAAGTTTCTCCAGTACCACAGGTTAGTGACCTGCACTGAAGCTCTTCTTCTCATGGATCAGAAAAACCATTCGAGCTTTCTTGATTTAACATCTCTGCTGGACATGAAAAATCTTGATTTGCTTCAAATTTCTGAGTATCCAAATTTGAAAGAAATAGACATTGATTGGGCAGGAGCAACTAATGATCCTCGGAACTCAATCATGACAAACCAAAGTTGCTACATTGCTCTTCAACACGTATCTGTAAGCTCTTGCTTCCAATTGAATGATATGACATGGCTCATTTTTGCTCAGAATCTCCTCAGATTATATGTATATGGCTGCTCCGGAATGCGGGAAATAATCAGTTCTCAAAGATTGGGTGCACATGTTGTAAGAGACATGAAACCATTTGCAAAACTTTCAGTTCTTGATTTGTGGGGTCTTCCGCTCTTGAGTAGCATATATACAAGTGCACTGCCATTTCCATATCTGAGTGAAATCAGAATATCTGGATGTCCAGCACTTAAAGAGTTGCCACTGAATTCCAGCAGTGCCCGAGGATGTAATCTCATCATCCAAGGAGAGGAATGGTGGTGGAATCGCTTACAATGGCCAAATGAAGATGCTCGAAATGCTTTCCTCCCCTGCTTCAGACATCGTAGTAGTAATCAGGTTCTTCCATGA

Protein Analysis

868

Amino Acids

98.4

Weight (kDa)

6.17

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 160 - 328 1.8e-50 NB-ARC domain
LRR_8 PF13855 503 - 561 5.4e-11 Leucine rich repeat
LRR_14 PF23598 523 - 702 4.9e-10 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12210 AT1G12210 AT1G12210 AT1G12220 AT1G12220 AT1G12220 AT1G12280 AT1G12280 AT1G12290 AT1G12290 AT1G12290 AT1G12290 AT1G15890 AT1G15890 AT1G51480 AT1G52660 AT1G52660 AT1G61180 AT1G61180 AT1G61190 AT1G61190 AT1G61190 AT1G61190 AT1G61300 AT1G61300 AT1G61310 AT3G15700 AT3G15700 AT4G10780 AT4G10780 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43740 AT5G43740 AT5G63020
fragaria_vesca FvH4_6g30050 FvH4_6g48220
malus_domestica MD09G1055800.v1.1 MD09G1198800.v1.1 MD17G1204800.v1.1 MD17G1204900.v1.1 MD17G1205000.v1.1 MD17G1205300.v1.1 MD17G1205700.v1.1 MD17G1205800.v1.1 MD17G1224100.v1.1
prunus_persica Prupe.3G054800_v2.0.a1 Prupe.3G054800_v2.0.a1 Prupe.3G080300_v2.0.a1 Prupe.3G080400_v2.0.a1 Prupe.3G080500_v2.0.a1 Prupe.3G080600_v2.0.a1 Prupe.3G080800_v2.0.a1 Prupe.3G083200_v2.0.a1 Prupe.3G099800_v2.0.a1 Prupe.3G099800_v2.0.a1
pyrus_communis pycom17g20860 pycom17g20910 pycom17g22790
rosa_chinensis RchiOBHm_Chr2g0136891 RchiOBHm_Chr2g0136901 RchiOBHm_Chr2g0167711 RchiOBHm_Chr2g0167721 RchiOBHm_Chr2g0167731 RchiOBHm_Chr4g0407611
rosa_laevigata RLG00000018943 RLG00000019278 RLG00000019282 RLG00000019587 RLG00000021748 RLG00000021749 RLG00000031057
rosa_multiflora Rmu_sc0002143.1_g000003 Rmu_sc0002782.1_g000001 Rmu_sc0023879.1_g000001 Rmu_sc0042171.1_g000001
rosa_roxburghii Rroxscaffold_2G00083300 Rroxscaffold_2G00107890 Rroxscaffold_2G00116680
rosa_rugosa Rorug02G0271700 Rorug02G0334900 Rorug02G0531800 Rorug02G0531800
rosa_samantha Rh2AG359900 Rh2AG385100 Rh2AG600500 Rh2AG600600 Rh2BG392200 Rh2BG611000 Rh2BG611100 Rh2BG611200 Rh2CG343100 Rh2CG343300 Rh2CG372300 Rh2CG581400 Rh2CG581500 Rh2DG382900 Rh2DG623700 Rh5AG027500 Rh5CG029900
rosa_wichuraiana Rw2G031490 Rw2G049850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1391
Acc36I ACCTGC 3 cut(s) 439, 1391, 1979
AccBSI CCGCTC 1 cut(s) 2371
AccII CGCG 3 cut(s) 877, 1338, 1624
AccIII TCCGGA 1 cut(s) 2267
AciI CCGC 8 cut(s) 202, 894, 1044, 1140, 1336, 1624, 2275, 2369
AclWI GGATC 7 cut(s) 641, 751, 1119, 1358, 1623, 2004, 2120
AcoI YGGCCR 1 cut(s) 2534
AcsI RAATTY 8 cut(s) 279, 1501, 1669, 1777, 1842, 2067, 2083, 2464
AcuI CTGAAG 5 cut(s) 158, 1122, 1176, 1998, 2557
AfaI GTAC 5 cut(s) 216, 864, 1233, 1927, 1955
AfiI CCNNNNNNNGG 7 cut(s) 342, 470, 1342, 1430, 1872, 2274, 2506
AflIII ACRYGT 3 cut(s) 875, 2176, 2308
AjnI CCWGG 1 cut(s) 1579
AloI GAACNNNNNNTCC 2 cut(s) 317, 349
Alw21I GWGCWC 2 cut(s) 2308, 2398
Alw26I GTCTC 4 cut(s) 242, 646, 701, 2313
Alw44I GTGCAC 2 cut(s) 2304, 2394
AlwI GGATC 7 cut(s) 641, 751, 1119, 1358, 1623, 2004, 2120
AlwNI CAGNNNCTG 2 cut(s) 1666, 1682
Ama87I CYCGRG 1 cut(s) 2478
Aor13HI TCCGGA 1 cut(s) 2267
AoxI GGCC 3 cut(s) 396, 1077, 2534
ApaLI GTGCAC 2 cut(s) 2304, 2394
ApeKI GCWGC 5 cut(s) 51, 184, 187, 1660, 2262
ApoI RAATTY 8 cut(s) 279, 1501, 1669, 1777, 1842, 2067, 2083, 2464
AseI ATTAAT 1 cut(s) 1061
Asp700I GAANNNNTTC 3 cut(s) 987, 1161, 1983
AspA2I CCTAGG 1 cut(s) 760
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 2 cut(s) 954, 1077
AsuHPI GGTGA 4 cut(s) 695, 808, 862, 1868
AsuII TTCGAA 1 cut(s) 1921
AvaI CYCGRG 1 cut(s) 2478
AvaII GGWCC 1 cut(s) 954
AvrII CCTAGG 1 cut(s) 760
BaeGI GKGCMC 3 cut(s) 2308, 2398, 2479
BalI TGGCCA 1 cut(s) 2536
BbsI GAAGAC 4 cut(s) 79, 569, 1100, 2357
Bbv12I GWGCWC 2 cut(s) 2308, 2398
BbvCI CCTCAGC 2 cut(s) 741, 1203
BbvI GCAGC 5 cut(s) 38, 196, 199, 1672, 2249
BccI CCATC 2 cut(s) 1192, 1553
BciT130I CCWGG 1 cut(s) 1581
BciVI GTATCC 1 cut(s) 2088
BcoDI GTCTC 4 cut(s) 242, 646, 701, 2313
BfaI CTAG 9 cut(s) 206, 276, 374, 761, 992, 1011, 1016, 1487, 1509
BfmI CTRYAG 1 cut(s) 340
BfuAI ACCTGC 3 cut(s) 439, 1391, 1979
BfuI GTATCC 1 cut(s) 2088
BglII AGATCT 1 cut(s) 219
BisI GCNGC 7 cut(s) 52, 185, 188, 203, 1045, 1661, 2263
BlnI CCTAGG 1 cut(s) 760
BlsI GCNGC 7 cut(s) 53, 186, 189, 204, 1046, 1662, 2264
BmcAI AGTACT 1 cut(s) 864
Bme1390I CCNGG 1 cut(s) 1581
Bme18I GGWCC 1 cut(s) 954
BmeT110I CYCGRG 1 cut(s) 2478
BmgT120I GGNCC 2 cut(s) 954, 1077
BmiI GGNNCC 1 cut(s) 503
BmrFI CCNGG 1 cut(s) 1581
BmsI GCATC 8 cut(s) 18, 25, 895, 947, 1151, 1492, 1672, 2536
BoxI GACNNNNGTC 1 cut(s) 254
BpiI GAAGAC 4 cut(s) 79, 569, 1100, 2357
BplI GAGNNNNNCTC 2 cut(s) 734, 766
BpmI CTGGAG 2 cut(s) 80, 1934
Bpu10I CCTNAGC 2 cut(s) 741, 1203
Bpu14I TTCGAA 1 cut(s) 1921
BpuEI CTTGAG 3 cut(s) 237, 1115, 2395
BsaAI YACGTR 1 cut(s) 2179
BsaBI GATNNNNATC 2 cut(s) 618, 632
BsaI GGTCTC 1 cut(s) 646
BsaJI CCNNGG 7 cut(s) 243, 645, 760, 1080, 2129, 2479, 2500
BsaWI WCCGGW 1 cut(s) 2267
Bsc4I CCNNNNNNNGG 7 cut(s) 342, 470, 1342, 1430, 1872, 2274, 2506
Bse1I ACTGG 2 cut(s) 1687, 1951
Bse3DI GCAATG 2 cut(s) 1655, 2163
Bse8I GATNNNNATC 2 cut(s) 618, 632
BseAI TCCGGA 1 cut(s) 2267
BseBI CCWGG 1 cut(s) 1581
BseDI CCNNGG 7 cut(s) 243, 645, 760, 1080, 2129, 2479, 2500
BseJI GATNNNNATC 2 cut(s) 618, 632
BseLI CCNNNNNNNGG 7 cut(s) 342, 470, 1342, 1430, 1872, 2274, 2506
BseMI GCAATG 2 cut(s) 1655, 2163
BseNI ACTGG 2 cut(s) 1687, 1951
BseRI GAGGAG 2 cut(s) 1718, 2231
BseSI GKGCMC 3 cut(s) 2308, 2398, 2479
BseXI GCAGC 5 cut(s) 38, 196, 199, 1672, 2249
BseYI CCCAGC 1 cut(s) 54
BsgI GTGCAG 2 cut(s) 1425, 1957
Bsh1236I CGCG 3 cut(s) 877, 1338, 1624
BshFI GGCC 3 cut(s) 398, 1079, 2536
BsiHKAI GWGCWC 2 cut(s) 2308, 2398
BsiHKCI CYCGRG 1 cut(s) 2478
BsiSI CCGG 2 cut(s) 399, 2268
BslI CCNNNNNNNGG 7 cut(s) 342, 470, 1342, 1430, 1872, 2274, 2506
BsmAI GTCTC 4 cut(s) 242, 646, 701, 2313
BsmI GAATGC 2 cut(s) 1917, 2277
BsnI GGCC 3 cut(s) 398, 1079, 2536
Bso31I GGTCTC 1 cut(s) 646
BsoBI CYCGRG 1 cut(s) 2478
Bsp119I TTCGAA 1 cut(s) 1921
Bsp1286I GDGCHC 3 cut(s) 2308, 2398, 2479
Bsp13I TCCGGA 1 cut(s) 2267
Bsp1407I TGTACA 1 cut(s) 1231
Bsp143I GATC 9 cut(s) 219, 627, 633, 756, 1111, 1363, 1615, 1996, 2125
Bsp19I CCATGG 2 cut(s) 243, 1080
BspACI CCGC 8 cut(s) 202, 894, 1044, 1140, 1336, 1624, 2275, 2369
BspANI GGCC 3 cut(s) 398, 1079, 2536
BspEI TCCGGA 1 cut(s) 2267
BspFNI CGCG 3 cut(s) 877, 1338, 1624
BspHI TCATGA 1 cut(s) 2142
BspLI GGNNCC 1 cut(s) 503
BspMI ACCTGC 3 cut(s) 439, 1391, 1979
BspPI GGATC 7 cut(s) 641, 751, 1119, 1358, 1623, 2004, 2120
BspQI GCTCTTC 4 cut(s) 993, 1436, 1989, 2175
BspT104I TTCGAA 1 cut(s) 1921
BspTNI GGTCTC 1 cut(s) 646
BsrBI CCGCTC 1 cut(s) 2371
BsrDI GCAATG 2 cut(s) 1655, 2163
BsrGI TGTACA 1 cut(s) 1231
BsrI ACTGG 2 cut(s) 1687, 1951
BssECI CCNNGG 7 cut(s) 243, 645, 760, 1080, 2129, 2479, 2500
BssMI GATC 9 cut(s) 219, 627, 633, 756, 1111, 1363, 1615, 1996, 2125
BssT1I CCWWGG 5 cut(s) 243, 645, 760, 1080, 2500
Bst2UI CCWGG 1 cut(s) 1581
Bst4CI ACNGT 3 cut(s) 300, 1069, 1193
Bst6I CTCTTC 6 cut(s) 513, 872, 993, 1436, 1989, 2175
BstAPI GCANNNNNTGC 1 cut(s) 855
BstAUI TGTACA 1 cut(s) 1231
BstBAI YACGTR 1 cut(s) 2179
BstBI TTCGAA 1 cut(s) 1921
BstC8I GCNNGC 6 cut(s) 7, 92, 1049, 1087, 1398, 1933
BstDSI CCRYGG 2 cut(s) 243, 1080
BstEII GGTNACC 1 cut(s) 426
BstFNI CGCG 3 cut(s) 877, 1338, 1624
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 9 cut(s) 222, 630, 636, 759, 1114, 1366, 1618, 1999, 2128
BstMAI GTCTC 4 cut(s) 242, 646, 701, 2313
BstMBI GATC 9 cut(s) 219, 627, 633, 756, 1111, 1363, 1615, 1996, 2125
BstMWI GCNNNNNNNGC 3 cut(s) 724, 855, 2555
BstNI CCWGG 1 cut(s) 1581
BstNSI RCATGY 4 cut(s) 1226, 1400, 1579, 2312
BstPAI GACNNNNGTC 1 cut(s) 254
BstPI GGTNACC 1 cut(s) 426
BstSCI CCNGG 1 cut(s) 1579
BstSFI CTRYAG 1 cut(s) 340
BstSLI GKGCMC 3 cut(s) 2308, 2398, 2479
BstUI CGCG 3 cut(s) 877, 1338, 1624
BstV1I GCAGC 5 cut(s) 38, 196, 199, 1672, 2249
BstV2I GAAGAC 4 cut(s) 79, 569, 1100, 2357
BstX2I RGATCY 2 cut(s) 219, 1615
BstYI RGATCY 2 cut(s) 219, 1615
BsuI GTATCC 1 cut(s) 2088
BsuRI GGCC 3 cut(s) 398, 1079, 2536
BtgI CCRYGG 2 cut(s) 243, 1080
BtsI GCAGTG 3 cut(s) 381, 2396, 2479
BtsIMutI CAGTG 5 cut(s) 381, 1974, 2396, 2459, 2479
BveI ACCTGC 3 cut(s) 439, 1391, 1979
Cac8I GCNNGC 6 cut(s) 7, 92, 1049, 1087, 1398, 1933
CaiI CAGNNNCTG 2 cut(s) 1666, 1682
CciI TCATGA 1 cut(s) 2142
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 2 cut(s) 954, 1077
CseI GACGC 1 cut(s) 707
Csp6I GTAC 5 cut(s) 215, 863, 1232, 1926, 1954
CviQI GTAC 5 cut(s) 215, 863, 1232, 1926, 1954
DpnI GATC 9 cut(s) 221, 629, 635, 758, 1113, 1365, 1617, 1998, 2127
DpnII GATC 9 cut(s) 219, 627, 633, 756, 1111, 1363, 1615, 1996, 2125
DraI TTTAAA 3 cut(s) 147, 390, 1587
EaeI YGGCCR 1 cut(s) 2534
Eam1104I CTCTTC 6 cut(s) 513, 872, 993, 1436, 1989, 2175
EarI CTCTTC 6 cut(s) 513, 872, 993, 1436, 1989, 2175
Eco130I CCWWGG 5 cut(s) 243, 645, 760, 1080, 2500
Eco31I GGTCTC 1 cut(s) 646
Eco32I GATATC 1 cut(s) 1006
Eco47I GGWCC 1 cut(s) 954
Eco57I CTGAAG 5 cut(s) 158, 1122, 1176, 1998, 2557
Eco88I CYCGRG 1 cut(s) 2478
Eco91I GGTNACC 1 cut(s) 426
EcoO65I GGTNACC 1 cut(s) 426
EcoRI GAATTC 3 cut(s) 279, 1501, 2464
EcoRII CCWGG 1 cut(s) 1579
EcoRV GATATC 1 cut(s) 1006
EcoT14I CCWWGG 5 cut(s) 243, 645, 760, 1080, 2500
EcoT22I ATGCAT 1 cut(s) 1326
ErhI CCWWGG 5 cut(s) 243, 645, 760, 1080, 2500
FauI CCCGC 1 cut(s) 2268
Fnu4HI GCNGC 7 cut(s) 52, 185, 188, 203, 1045, 1661, 2263
Fsp4HI GCNGC 7 cut(s) 52, 185, 188, 203, 1045, 1661, 2263
FspBI CTAG 9 cut(s) 206, 276, 374, 761, 992, 1011, 1016, 1487, 1509
GlaI GCGC 1 cut(s) 435
GluI GCNGC 7 cut(s) 52, 185, 188, 203, 1045, 1661, 2263
GsaI CCCAGC 1 cut(s) 58
GsuI CTGGAG 2 cut(s) 80, 1934
HaeIII GGCC 3 cut(s) 398, 1079, 2536
HapII CCGG 2 cut(s) 399, 2268
HgaI GACGC 1 cut(s) 707
HhaI GCGC 1 cut(s) 436
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HincII GTYRAC 2 cut(s) 1518, 1908
HindII GTYRAC 2 cut(s) 1518, 1908
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 6 cut(s) 59, 250, 479, 1763, 2236, 2521
HpaII CCGG 2 cut(s) 399, 2268
HphI GGTGA 4 cut(s) 695, 808, 862, 1868
Hpy166II GTNNAC 6 cut(s) 259, 1518, 1749, 1908, 2306, 2396
Hpy8I GTNNAC 6 cut(s) 259, 1518, 1749, 1908, 2306, 2396
HpyAV CCTTC 4 cut(s) 49, 581, 1232, 1847
HpyCH4III ACNGT 3 cut(s) 300, 1069, 1193
HpyCH4IV ACGT 3 cut(s) 122, 888, 2178
HpyF10VI GCNNNNNNNGC 3 cut(s) 724, 855, 2555
HpySE526I ACGT 3 cut(s) 122, 888, 2178
HspAI GCGC 1 cut(s) 434
Kpn2I TCCGGA 1 cut(s) 2267
Kzo9I GATC 9 cut(s) 219, 627, 633, 756, 1111, 1363, 1615, 1996, 2125
LguI GCTCTTC 4 cut(s) 993, 1436, 1989, 2175
LmnI GCTCC 6 cut(s) 99, 501, 802, 1480, 2114, 2270
Lsp1109I GCAGC 5 cut(s) 38, 196, 199, 1672, 2249
LweI GCATC 8 cut(s) 18, 25, 895, 947, 1151, 1492, 1672, 2536
MaeI CTAG 9 cut(s) 206, 276, 374, 761, 992, 1011, 1016, 1487, 1509
MaeII ACGT 3 cut(s) 122, 888, 2178
MaeIII GTNAC 3 cut(s) 426, 1253, 1966
MalI GATC 9 cut(s) 221, 629, 635, 758, 1113, 1365, 1617, 1998, 2127
MbiI CCGCTC 1 cut(s) 2371
MboI GATC 9 cut(s) 219, 627, 633, 756, 1111, 1363, 1615, 1996, 2125
MfeI CAATTG 2 cut(s) 465, 2201
MflI RGATCY 2 cut(s) 219, 1615
MhlI GDGCHC 3 cut(s) 2308, 2398, 2479
MlsI TGGCCA 1 cut(s) 2536
MluI ACGCGT 1 cut(s) 875
MluNI TGGCCA 1 cut(s) 2536
MlyI GAGTC 1 cut(s) 244
MmeI TCCRAC 5 cut(s) 49, 955, 1175, 1272, 1411
Mox20I TGGCCA 1 cut(s) 2536
Mph1103I ATGCAT 1 cut(s) 1326
MroI TCCGGA 1 cut(s) 2267
MroXI GAANNNNTTC 3 cut(s) 987, 1161, 1983
MscI TGGCCA 1 cut(s) 2536
MseI TTAA 9 cut(s) 146, 389, 921, 1061, 1187, 1278, 1586, 2027, 2448
MslI CAYNNNNRTG 4 cut(s) 752, 1227, 1401, 1488
Msp20I TGGCCA 1 cut(s) 2536
MspI CCGG 2 cut(s) 399, 2268
MspR9I CCNGG 1 cut(s) 1581
MunI CAATTG 2 cut(s) 465, 2201
Mva1269I GAATGC 2 cut(s) 1917, 2277
MvaI CCWGG 1 cut(s) 1581
MvnI CGCG 3 cut(s) 877, 1338, 1624
MwoI GCNNNNNNNGC 3 cut(s) 724, 855, 2555
NcoI CCATGG 2 cut(s) 243, 1080
NdeII GATC 9 cut(s) 219, 627, 633, 756, 1111, 1363, 1615, 1996, 2125
NlaIV GGNNCC 1 cut(s) 503
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 1 cut(s) 1966
NsiI ATGCAT 1 cut(s) 1326
NspI RCATGY 4 cut(s) 1226, 1400, 1579, 2312
NspV TTCGAA 1 cut(s) 1921
PaeI GCATGC 1 cut(s) 1400
PagI TCATGA 1 cut(s) 2142
PaqCI CACCTGC 1 cut(s) 1391
PciI ACATGT 1 cut(s) 2308
PciSI GCTCTTC 4 cut(s) 993, 1436, 1989, 2175
PctI GAATGC 2 cut(s) 1917, 2277
PdmI GAANNNNTTC 3 cut(s) 987, 1161, 1983
PfeI GAWTC 5 cut(s) 59, 479, 1763, 2236, 2521
PflFI GACNNNGTC 1 cut(s) 121
PkrI GCNGC 7 cut(s) 53, 186, 189, 204, 1046, 1662, 2264
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
Ppu21I YACGTR 1 cut(s) 2179
PscI ACATGT 1 cut(s) 2308
PshAI GACNNNNGTC 1 cut(s) 254
PshBI ATTAAT 1 cut(s) 1061
Psp6I CCWGG 1 cut(s) 1579
PspEI GGTNACC 1 cut(s) 426
PspFI CCCAGC 1 cut(s) 54
PspGI CCWGG 1 cut(s) 1579
PspN4I GGNNCC 1 cut(s) 503
PspPI GGNCC 2 cut(s) 954, 1077
PstNI CAGNNNCTG 2 cut(s) 1666, 1682
PsuI RGATCY 2 cut(s) 219, 1615
PsyI GACNNNGTC 1 cut(s) 121
RsaI GTAC 5 cut(s) 216, 864, 1233, 1927, 1955
RsaNI GTAC 5 cut(s) 215, 863, 1232, 1926, 1954
RseI CAYNNNNRTG 4 cut(s) 752, 1227, 1401, 1488
SapI GCTCTTC 4 cut(s) 993, 1436, 1989, 2175
SaqAI TTAA 9 cut(s) 146, 389, 921, 1061, 1187, 1278, 1586, 2027, 2448
SatI GCNGC 7 cut(s) 52, 185, 188, 203, 1045, 1661, 2263
Sau3AI GATC 9 cut(s) 219, 627, 633, 756, 1111, 1363, 1615, 1996, 2125
Sau96I GGNCC 2 cut(s) 954, 1077
ScaI AGTACT 1 cut(s) 864
SchI GAGTC 1 cut(s) 244
ScrFI CCNGG 1 cut(s) 1581
SduI GDGCHC 3 cut(s) 2308, 2398, 2479
SfaNI GCATC 8 cut(s) 18, 25, 895, 947, 1151, 1492, 1672, 2536
SfcI CTRYAG 1 cut(s) 340
SfuI TTCGAA 1 cut(s) 1921
SinI GGWCC 1 cut(s) 954
SmiMI CAYNNNNRTG 4 cut(s) 752, 1227, 1401, 1488
SmlI CTYRAG 3 cut(s) 252, 1130, 2374
SmoI CTYRAG 3 cut(s) 252, 1130, 2374
SphI GCATGC 1 cut(s) 1400
SsiI CCGC 8 cut(s) 202, 894, 1044, 1140, 1336, 1624, 2275, 2369
SspI AATATT 1 cut(s) 1733
SspMI CTAG 9 cut(s) 206, 276, 374, 761, 992, 1011, 1016, 1487, 1509
StyD4I CCNGG 1 cut(s) 1579
StyI CCWWGG 5 cut(s) 243, 645, 760, 1080, 2500
TaaI ACNGT 3 cut(s) 300, 1069, 1193
TaiI ACGT 3 cut(s) 125, 891, 2181
TaqI TCGA 4 cut(s) 510, 1921, 2012, 2551
TatI WGTACW 3 cut(s) 862, 1231, 1925
TauI GCSGC 2 cut(s) 205, 1047
TfiI GAWTC 5 cut(s) 59, 479, 1763, 2236, 2521
Tru1I TTAA 9 cut(s) 146, 389, 921, 1061, 1187, 1278, 1586, 2027, 2448
Tru9I TTAA 9 cut(s) 146, 389, 921, 1061, 1187, 1278, 1586, 2027, 2448
TscAI CASTG 5 cut(s) 388, 1981, 2403, 2466, 2479
TseFI GTSAC 1 cut(s) 1966
TseI GCWGC 5 cut(s) 51, 184, 187, 1660, 2262
Tsp45I GTSAC 1 cut(s) 1966
TspRI CASTG 5 cut(s) 388, 1981, 2403, 2466, 2479
Tth111I GACNNNGTC 1 cut(s) 121
VneI GTGCAC 2 cut(s) 2304, 2394
VpaK11BI GGWCC 1 cut(s) 954
VspI ATTAAT 1 cut(s) 1061
XapI RAATTY 8 cut(s) 279, 1501, 1669, 1777, 1842, 2067, 2083, 2464
XbaI TCTAGA 1 cut(s) 1486
XceI RCATGY 4 cut(s) 1226, 1400, 1579, 2312
XmaJI CCTAGG 1 cut(s) 760
XmnI GAANNNNTTC 3 cut(s) 987, 1161, 1983
XspI CTAG 9 cut(s) 206, 276, 374, 761, 992, 1011, 1016, 1487, 1509
ZrmI AGTACT 1 cut(s) 864
Zsp2I ATGCAT 1 cut(s) 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.