MD17G1204800.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000363
Physical Location & Seq
Reverse (-)
24782329 .. 24785052
2724 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1204800.v1.1.491

Sequence Viewer

Length: 2724 bp
ATGGGGAACATGTTCTCAGTCTCCATACCTTGCGATCACACGGCTAACTTCTGCTGCTGGGGTTTCCTTAGTCAACAAGCAAACTACATCGGCAAGCTCGGAGTAAACCTTGAAGCTCTGGATCGATCATCGCAAGAACTGATCGCTTTAAAGAATGATCTCGTGAGAAGGGTTGAGGTTTCCGAGCTTCAGACATCCATGATGCGCTTGGACCAGGTACAACTGTGGATTAAAAAGGCGGAAACTCTTGAGACACGAGTCAGAGAAGTTAAAAATTGTGCAGATCGAGAAATTCAGAAATCATGTAGCGGAGTTTACTGCTCCAAGAACTACTTTCCCAGCTACAAGTACGGAAAAAAAGTGTTTAAAATGTTGGTGGAAGTGGATGCCTTGAAAAGCAAGGGAAGCTTTGAAGAGGTGGTAACAGAAAGCTTACCGGTAGGTGTAGTCTATGAAATGCCTACTGAACCAACAGTGGGGATGGAGTCGATGTTTGAACAGGTCTGGAGACATGTTGAAGATGAACAGGTGGGAACAATCGGGTTATATGGAATGGGAGGGGTGGGCAAGACCACCCTCCTTACCAAAATCCATAACGATTTGGCCTTTAGTACTTCTTACCATTTTGATCTTGTGCTTTGGATCGTGGTCTCAAAAGACCACAAACTTGAAATCCTCCAAGATAAGATTGGTGAAAAGCTTGGGATTGTTAATGACAAATGGAAGCACAAAGAGCCGCATGAAAAAGCTCAACACATCTTCAAGTTCTTGAGTGAGAAGATATTTGTGCTATTGTTGGATGATTTATGGGAGCCGATTGAAATGATTAAAGTTGGGATTCCTAATCCAGCGATTCCTGATACAGCTGACAATAAGAAGTCGAAGAAGTCGAAGATAATATTCACGACTCGCTTGGAGCATATATGTAGTTGTATGGATGCTCAAATAAAGATTAAAGTCAGGTGTCTAATGCCGAACCAAGCATGGACCTTGTTTCAGGAGAAGGTGGGCAAAGAAACTCTTCAAAGTCATCCAGATATCCCCGCACTTGCGCAAGTCGTGGCAAATGAGTGTGACGGTTTGCCACTTGCATCGATTACAGTTGGTCAAGCCATGACGTGCAAGAAAACACCCCACGAATGGAATCATGCAATTCGGGTTCTAAAGGAATCTGCCTCAGAGTTTTCCACTATGGGAGACAAGGTATTTCCTCTTTTAAAATTTAGTTACGATAATCTGCCCAGTGAGACAATCAAATCATGCTTCTTATATTGTGCTCTATTTCCGGAAGATTTCCGGATGAGTAAAGATGATTTATTATATTTTTGGATGTGTGAGGACATGTTTGAAGAGTATGTTAACGTAGATGTAGCGAAAAATGAGACTTACCATATCATAGGAACGCTCCTTTCTGCATGTTTGTTGGAAGAAGAAGGAAATTTTGTAAAAATGCATGACGTAATTCGTGACATGGCATTGTGGTTAGCTTGTGACTGCGGGAAAGAAAACGAGAGTATCCTCGTGCATGCAGGTCTCCAGGGAACACCACATGTGAAGAAATGGAAGAACGCTAAAAGGGTATCATTGGTTGGTAGTCATTTTAAAAGCCTAGTTGAAACACCAAAATCTCCAAATCTGTTGACCTTATTTCTTAGAGGTGGAAAAGGTCCTTTGAATATGATTGCTGATGGCTTTTTCGATCATATGCCTACTCTGCGAGTTCTGGATTTGTCTGAAAATCTGAACATAACCAAATTGCCATCTGGCATTTCCAACCTGATTTCGTTACAACACCTAAATTTGTCAAGAACTGGTGTAAGAGAGCTGCCAATTGAATTAAAGGCTTGTGTGAGGCTAAAATATTTGGATTTGGAGCATACGGATCAGCTTGATTTTGTACCACAAAATATAATATCAAGTTTTCCAATGCTAAAAGTTTTGAGGATGCTAGCATGTAGTTCGTCAGATAGAATTCTTGTTGACGGTGAAGGAGCCCTGATGGAGGAAATGCAGGATTTGGAATACCTTGACGTTTTGACTTTGAGCGTACGGAGTAGCTCTTTTTTGCAAAAACTGATCGGATTCTACAAATTGGTGACCCGCATTCGGACTCTACAGCTCTCGGATGGCAAGAACGAAGCAAGCTTTCTAGACATATCGTCTTTGGTGGATATGAAACATCTTGATACCCTTTATATTCGGGATACTCCTAATTGGAAACAATTGGAAGTTGATTGGGCAGGACGAGCACCTAACGATCCCTGGGATTTAATGATGATAAAACAAAATTGCTTCCTTTGCCTTCAGTTCATAGAAGTATCACGATGCCCAAATCTCACGGACATGACTTGGCTCATTTTTGCTCCGAATCTCAGCCATTTACGTGTAGATGCCTGCAATGATATTGAGAAAATAATCGATTTGGAAAGACTGGGTGGAGTTGGAAATGTGGTACAAGAACTGAACCCTTTTGGAAAACTTACCATTCTGATTTTGAGTATGCTTCCACGGCTGAGTAGCATACATGATAGTCCCCTGCCCTTTCCATACCTGAAAGAAATCAAGGTAGTTGCATGTCCAGTGCTGAGAAAGCTTCCACTCAACTCCGCAAGTGCTGAGGGGAGCAATATTGTAATCAGAGGACATGAAGCATGGTGGTCTTTCTTAGAGTGGGAGGACGAAGATGCTCAAAATGTTTTTCTTCCCTGCTTCAGACATGGTTAA

Protein Analysis

908

Amino Acids

103.1

Weight (kDa)

5.83

Isoelectric Point (pI)

41.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 162 - 341 9.8e-42 NB-ARC domain
WHD_DRP PF23559 427 - 492 3.5e-06 Disease resistance protein Winged helix domain
LRR_8 PF13855 545 - 604 5.2e-08 Leucine rich repeat
LRR_14 PF23598 569 - 812 5.6e-14 Leucine-rich repeat region
LRR_RPS2 PF23247 759 - 865 3.3e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12210 AT1G12210 AT1G12210 AT1G12220 AT1G12220 AT1G12220 AT1G12280 AT1G12280 AT1G12290 AT1G12290 AT1G12290 AT1G12290 AT1G15890 AT1G15890 AT1G51480 AT1G52660 AT1G52660 AT1G61180 AT1G61180 AT1G61190 AT1G61190 AT1G61190 AT1G61190 AT1G61300 AT1G61300 AT1G61310 AT3G15700 AT3G15700 AT4G10780 AT4G10780 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43740 AT5G43740 AT5G63020
fragaria_vesca FvH4_6g30050 FvH4_6g48220
malus_domestica MD09G1055800.v1.1 MD09G1198800.v1.1 MD17G1204800.v1.1 MD17G1204900.v1.1 MD17G1205000.v1.1 MD17G1205300.v1.1 MD17G1205700.v1.1 MD17G1205800.v1.1 MD17G1224100.v1.1
prunus_persica Prupe.3G054800_v2.0.a1 Prupe.3G054800_v2.0.a1 Prupe.3G080300_v2.0.a1 Prupe.3G080400_v2.0.a1 Prupe.3G080500_v2.0.a1 Prupe.3G080600_v2.0.a1 Prupe.3G080800_v2.0.a1 Prupe.3G083200_v2.0.a1 Prupe.3G099800_v2.0.a1 Prupe.3G099800_v2.0.a1
pyrus_communis pycom17g20860 pycom17g20910 pycom17g22790
rosa_chinensis RchiOBHm_Chr2g0136891 RchiOBHm_Chr2g0136901 RchiOBHm_Chr2g0167711 RchiOBHm_Chr2g0167721 RchiOBHm_Chr2g0167731 RchiOBHm_Chr4g0407611
rosa_laevigata RLG00000018943 RLG00000019278 RLG00000019282 RLG00000019587 RLG00000021748 RLG00000021749 RLG00000031057
rosa_multiflora Rmu_sc0002143.1_g000003 Rmu_sc0002782.1_g000001 Rmu_sc0023879.1_g000001 Rmu_sc0042171.1_g000001
rosa_roxburghii Rroxscaffold_2G00083300 Rroxscaffold_2G00107890 Rroxscaffold_2G00116680
rosa_rugosa Rorug02G0271700 Rorug02G0334900 Rorug02G0531800 Rorug02G0531800
rosa_samantha Rh2AG359900 Rh2AG385100 Rh2AG600500 Rh2AG600600 Rh2BG392200 Rh2BG611000 Rh2BG611100 Rh2BG611200 Rh2CG343100 Rh2CG343300 Rh2CG372300 Rh2CG581400 Rh2CG581500 Rh2DG382900 Rh2DG623700 Rh5AG027500 Rh5CG029900
rosa_wichuraiana Rw2G031490 Rw2G049850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1053
Acc36I ACCTGC 1 cut(s) 1520
AccIII TCCGGA 2 cut(s) 1285, 1296
AciI CCGC 7 cut(s) 239, 309, 737, 1044, 1497, 2101, 2607
AclWI GGATC 4 cut(s) 129, 650, 1890, 2252
AcsI RAATTY 5 cut(s) 291, 1220, 1438, 1798, 1971
AcuI CTGAAG 3 cut(s) 173, 2288, 2695
AfaI GTAC 6 cut(s) 219, 350, 613, 1899, 2049, 2454
AfiI CCNNNNNNNGG 4 cut(s) 476, 1140, 2002, 2106
AflIII ACRYGT 5 cut(s) 9, 511, 1341, 1549, 2383
AgeI ACCGGT 1 cut(s) 436
AhdI GACNNNNNGTC 1 cut(s) 2158
AjiI CACGTC 1 cut(s) 1119
AjnI CCWGG 3 cut(s) 213, 1536, 2261
Alw21I GWGCWC 2 cut(s) 1279, 2251
Alw26I GTCTC 8 cut(s) 25, 245, 502, 655, 1191, 1241, 1376, 1538
AlwI GGATC 4 cut(s) 129, 650, 1890, 2252
Aor13HI TCCGGA 2 cut(s) 1285, 1296
AoxI GGCC 1 cut(s) 603
ApeKI GCWGC 2 cut(s) 54, 1825
ApoI RAATTY 5 cut(s) 291, 1220, 1438, 1798, 1971
AsiGI ACCGGT 1 cut(s) 436
Asp700I GAANNNNTTC 2 cut(s) 11, 1020
AspLEI GCGC 2 cut(s) 207, 1054
AspS9I GGNCC 3 cut(s) 211, 987, 1667
AsuHPI GGTGA 3 cut(s) 704, 1997, 2107
AsuNHI GCTAGC 1 cut(s) 1948
AvaII GGWCC 3 cut(s) 211, 987, 1667
BaeI ACNNNNGTAYC 4 cut(s) 209, 242, 1498, 1531
BanII GRGCYC 1 cut(s) 1996
BauI CACGAG 3 cut(s) 161, 255, 1520
Bbv12I GWGCWC 2 cut(s) 1279, 2251
BbvCI CCTCAGC 1 cut(s) 2616
BbvI GCAGC 2 cut(s) 41, 1812
BccI CCATC 5 cut(s) 475, 1682, 1768, 1993, 2120
BceAI ACGGC 2 cut(s) 57, 2525
BcgI CGANNNNNNTGC 2 cut(s) 1941, 1975
BciT130I CCWGG 3 cut(s) 215, 1538, 2263
BciVI GTATCC 2 cut(s) 1526, 2197
BcoDI GTCTC 8 cut(s) 25, 245, 502, 655, 1191, 1241, 1376, 1538
BfaI CTAG 3 cut(s) 1610, 1949, 2150
BfmI CTRYAG 1 cut(s) 2114
BfuAI ACCTGC 1 cut(s) 1520
BfuI GTATCC 2 cut(s) 1526, 2197
BisI GCNGC 3 cut(s) 55, 737, 1826
BlsI GCNGC 3 cut(s) 56, 738, 1827
BmcAI AGTACT 1 cut(s) 613
Bme1390I CCNGG 3 cut(s) 215, 1538, 2263
Bme18I GGWCC 3 cut(s) 211, 987, 1667
BmeRI GACNNNNNGTC 1 cut(s) 2158
BmgBI CACGTC 1 cut(s) 1119
BmgT120I GGNCC 3 cut(s) 211, 987, 1667
BmiI GGNNCC 2 cut(s) 813, 1993
BmrFI CCNGG 3 cut(s) 215, 1538, 2263
BmrI ACTGGG 2 cut(s) 1236, 2441
BmsI GCATC 8 cut(s) 192, 376, 928, 1100, 1935, 2315, 2380, 2674
BmtI GCTAGC 1 cut(s) 1952
BmuI ACTGGG 2 cut(s) 1236, 2441
BoxI GACNNNNGTC 1 cut(s) 257
BpmI CTGGAG 2 cut(s) 526, 1520
Bpu10I CCTNAGC 1 cut(s) 2616
BpuEI CTTGAG 2 cut(s) 269, 790
Bsa29I ATCGAT 3 cut(s) 124, 1094, 2418
BsaAI YACGTR 1 cut(s) 2384
BsaI GGTCTC 2 cut(s) 655, 1538
BsaJI CCNNGG 4 cut(s) 1537, 2261, 2262, 2507
BsaWI WCCGGW 3 cut(s) 436, 1285, 1296
BsaXI ACNNNNNCTCC 4 cut(s) 476, 506, 1188, 1218
Bsc4I CCNNNNNNNGG 4 cut(s) 476, 1140, 2002, 2106
Bse118I RCCGGY 1 cut(s) 436
Bse1I ACTGG 4 cut(s) 1242, 1816, 2436, 2579
Bse3DI GCAATG 1 cut(s) 2404
BseAI TCCGGA 2 cut(s) 1285, 1296
BseBI CCWGG 3 cut(s) 215, 1538, 2263
BseCI ATCGAT 3 cut(s) 124, 1094, 2418
BseDI CCNNGG 4 cut(s) 1537, 2261, 2262, 2507
BseLI CCNNNNNNNGG 4 cut(s) 476, 1140, 2002, 2106
BseMI GCAATG 1 cut(s) 2404
BseMII CTCAG 6 cut(s) 30, 1191, 2386, 2504, 2576, 2607
BseNI ACTGG 4 cut(s) 1242, 1816, 2436, 2579
BseXI GCAGC 2 cut(s) 41, 1812
BseYI CCCAGC 2 cut(s) 57, 338
BsgI GTGCAG 1 cut(s) 300
BshFI GGCC 1 cut(s) 605
BshTI ACCGGT 1 cut(s) 436
BshVI ATCGAT 3 cut(s) 124, 1094, 2418
BsiHKAI GWGCWC 2 cut(s) 1279, 2251
BsiSI CCGG 3 cut(s) 437, 1286, 1297
BsiWI CGTACG 1 cut(s) 2047
BslFI GGGAC 1 cut(s) 2517
BslI CCNNNNNNNGG 4 cut(s) 476, 1140, 2002, 2106
BsmAI GTCTC 8 cut(s) 25, 245, 502, 655, 1191, 1241, 1376, 1538
BsmFI GGGAC 1 cut(s) 2517
BsmI GAATGC 1 cut(s) 2103
BsnI GGCC 1 cut(s) 605
Bso31I GGTCTC 2 cut(s) 655, 1538
Bsp1286I GDGCHC 3 cut(s) 1279, 1996, 2251
Bsp13I TCCGGA 2 cut(s) 1285, 1296
BspACI CCGC 7 cut(s) 239, 309, 737, 1044, 1497, 2101, 2607
BspANI GGCC 1 cut(s) 605
BspCNI CTCAG 6 cut(s) 29, 1190, 2385, 2505, 2577, 2608
BspDI ATCGAT 3 cut(s) 124, 1094, 2418
BspEI TCCGGA 2 cut(s) 1285, 1296
BspLI GGNNCC 2 cut(s) 813, 1993
BspMI ACCTGC 1 cut(s) 1520
BspOI GCTAGC 1 cut(s) 1952
BspPI GGATC 4 cut(s) 129, 650, 1890, 2252
BspTNI GGTCTC 2 cut(s) 655, 1538
BsrDI GCAATG 1 cut(s) 2404
BsrFI RCCGGY 1 cut(s) 436
BsrI ACTGG 4 cut(s) 1242, 1816, 2436, 2579
BssAI RCCGGY 1 cut(s) 436
BssECI CCNNGG 4 cut(s) 1537, 2261, 2262, 2507
BssSI CACGAG 3 cut(s) 161, 255, 1520
Bst2BI CACGAG 3 cut(s) 161, 255, 1520
Bst2UI CCWGG 3 cut(s) 215, 1538, 2263
Bst4CI ACNGT 5 cut(s) 225, 475, 1079, 1102, 1985
Bst6I CTCTTC 3 cut(s) 408, 1026, 1344
BstBAI YACGTR 1 cut(s) 2384
BstC8I GCNNGC 5 cut(s) 95, 1527, 1950, 2143, 2395
BstDEI CTNAG 9 cut(s) 16, 68, 1177, 1652, 2372, 2513, 2585, 2616, 2665
BstDSI CCRYGG 1 cut(s) 2507
BstEII GGTNACC 1 cut(s) 2095
BstENI CCTNNNNNAGG 1 cut(s) 2000
BstHHI GCGC 2 cut(s) 207, 1054
BstMAI GTCTC 8 cut(s) 25, 245, 502, 655, 1191, 1241, 1376, 1538
BstMWI GCNNNNNNNGC 7 cut(s) 405, 733, 1714, 2246, 2298, 2509, 2590
BstNI CCWGG 3 cut(s) 215, 1538, 2263
BstNSI RCATGY 8 cut(s) 13, 515, 1345, 1419, 1529, 1553, 1956, 2577
BstPAI GACNNNNGTC 1 cut(s) 257
BstPI GGTNACC 1 cut(s) 2095
BstSCI CCNGG 3 cut(s) 213, 1536, 2261
BstSFI CTRYAG 1 cut(s) 2114
BstV1I GCAGC 2 cut(s) 41, 1812
Bsu15I ATCGAT 3 cut(s) 124, 1094, 2418
BsuI GTATCC 2 cut(s) 1526, 2197
BsuRI GGCC 1 cut(s) 605
BsuTUI ATCGAT 3 cut(s) 124, 1094, 2418
BtgI CCRYGG 1 cut(s) 2507
BtgZI GCGATG 1 cut(s) 114
BtrI CACGTC 1 cut(s) 1119
BtsIMutI CAGTG 3 cut(s) 480, 1249, 2586
BveI ACCTGC 1 cut(s) 1520
Cac8I GCNNGC 5 cut(s) 95, 1527, 1950, 2143, 2395
CfoI GCGC 2 cut(s) 207, 1054
Cfr10I RCCGGY 1 cut(s) 436
Cfr13I GGNCC 3 cut(s) 211, 987, 1667
ClaI ATCGAT 3 cut(s) 124, 1094, 2418
CsiI ACCWGGT 1 cut(s) 213
Csp6I GTAC 6 cut(s) 218, 349, 612, 1898, 2048, 2453
CspAI ACCGGT 1 cut(s) 436
CviQI GTAC 6 cut(s) 218, 349, 612, 1898, 2048, 2453
DdeI CTNAG 9 cut(s) 16, 68, 1177, 1652, 2372, 2513, 2585, 2616, 2665
DraI TTTAAA 4 cut(s) 150, 367, 1218, 1603
DriI GACNNNNNGTC 1 cut(s) 2158
Eam1104I CTCTTC 3 cut(s) 408, 1026, 1344
Eam1105I GACNNNNNGTC 1 cut(s) 2158
EarI CTCTTC 3 cut(s) 408, 1026, 1344
EciI GGCGGA 1 cut(s) 254
Eco24I GRGCYC 1 cut(s) 1996
Eco31I GGTCTC 2 cut(s) 655, 1538
Eco32I GATATC 1 cut(s) 1039
Eco47I GGWCC 3 cut(s) 211, 987, 1667
Eco57I CTGAAG 3 cut(s) 173, 2288, 2695
Eco91I GGTNACC 1 cut(s) 2095
EcoNI CCTNNNNNAGG 1 cut(s) 2000
EcoO109I RGGNCCY 1 cut(s) 1667
EcoO65I GGTNACC 1 cut(s) 2095
EcoRI GAATTC 1 cut(s) 1971
EcoRII CCWGG 3 cut(s) 213, 1536, 2261
EcoRV GATATC 1 cut(s) 1039
EcoT22I ATGCAT 1 cut(s) 1455
EcoT38I GRGCYC 1 cut(s) 1996
FalI AAGNNNNNCTT 6 cut(s) 317, 349, 392, 424, 1005, 1037
FaqI GGGAC 1 cut(s) 2517
FauI CCCGC 3 cut(s) 1051, 1490, 2108
FauNDI CATATG 1 cut(s) 1704
Fnu4HI GCNGC 3 cut(s) 55, 737, 1826
FriOI GRGCYC 1 cut(s) 1996
Fsp4HI GCNGC 3 cut(s) 55, 737, 1826
FspBI CTAG 3 cut(s) 1610, 1949, 2150
FspI TGCGCA 1 cut(s) 1053
GlaI GCGC 2 cut(s) 206, 1053
GluI GCNGC 3 cut(s) 55, 737, 1826
GsaI CCCAGC 2 cut(s) 61, 342
GsuI CTGGAG 2 cut(s) 526, 1520
HaeIII GGCC 1 cut(s) 605
HapII CCGG 3 cut(s) 437, 1286, 1297
HhaI GCGC 2 cut(s) 207, 1054
Hin6I GCGC 2 cut(s) 205, 1052
HinP1I GCGC 2 cut(s) 205, 1052
HincII GTYRAC 4 cut(s) 74, 1360, 1641, 1981
HindII GTYRAC 4 cut(s) 74, 1360, 1641, 1981
HindIII AAGCTT 5 cut(s) 406, 430, 698, 2143, 2591
HpaI GTTAAC 1 cut(s) 1360
HpaII CCGG 3 cut(s) 437, 1286, 1297
HphI GGTGA 3 cut(s) 704, 1997, 2107
Hpy166II GTNNAC 6 cut(s) 74, 106, 316, 1360, 1641, 1981
Hpy8I GTNNAC 6 cut(s) 74, 106, 316, 1360, 1641, 1981
HpyAV CCTTC 5 cut(s) 162, 997, 1427, 1982, 2312
HpyCH4III ACNGT 5 cut(s) 225, 475, 1079, 1102, 1985
HpyCH4IV ACGT 5 cut(s) 1118, 1362, 1458, 2031, 2383
HpyF10VI GCNNNNNNNGC 7 cut(s) 405, 733, 1714, 2246, 2298, 2509, 2590
HpyF3I CTNAG 9 cut(s) 16, 68, 1177, 1652, 2372, 2513, 2585, 2616, 2665
HpySE526I ACGT 5 cut(s) 1118, 1362, 1458, 2031, 2383
HspAI GCGC 2 cut(s) 205, 1052
Kpn2I TCCGGA 2 cut(s) 1285, 1296
KspAI GTTAAC 1 cut(s) 1360
LmnI GCTCC 8 cut(s) 326, 811, 916, 1410, 1873, 1991, 2368, 2622
Lsp1109I GCAGC 2 cut(s) 41, 1812
LweI GCATC 8 cut(s) 192, 376, 928, 1100, 1935, 2315, 2380, 2674
MabI ACCWGGT 1 cut(s) 213
MaeI CTAG 3 cut(s) 1610, 1949, 2150
MaeII ACGT 5 cut(s) 1118, 1362, 1458, 2031, 2383
MaeIII GTNAC 7 cut(s) 421, 1073, 1226, 1466, 1490, 1785, 2095
MfeI CAATTG 2 cut(s) 1830, 2222
MhlI GDGCHC 3 cut(s) 1279, 1996, 2251
MlyI GAGTC 4 cut(s) 267, 494, 901, 2104
MmeI TCCRAC 4 cut(s) 777, 1404, 1797, 2422
Mph1103I ATGCAT 1 cut(s) 1455
MroI TCCGGA 2 cut(s) 1285, 1296
MroXI GAANNNNTTC 2 cut(s) 11, 1020
MslI CAYNNNNRTG 2 cut(s) 2342, 2382
MspA1I CMGCKG 1 cut(s) 866
MspI CCGG 3 cut(s) 437, 1286, 1297
MspR9I CCNGG 3 cut(s) 215, 1538, 2263
MunI CAATTG 2 cut(s) 1830, 2222
Mva1269I GAATGC 1 cut(s) 2103
MvaI CCWGG 3 cut(s) 215, 1538, 2263
MwoI GCNNNNNNNGC 7 cut(s) 405, 733, 1714, 2246, 2298, 2509, 2590
NdeI CATATG 1 cut(s) 1704
NheI GCTAGC 1 cut(s) 1948
NlaIV GGNNCC 2 cut(s) 813, 1993
NmuCI GTSAC 4 cut(s) 1073, 1466, 1490, 2095
NsbI TGCGCA 1 cut(s) 1053
NsiI ATGCAT 1 cut(s) 1455
NspI RCATGY 8 cut(s) 13, 515, 1345, 1419, 1529, 1553, 1956, 2577
PaeI GCATGC 1 cut(s) 1529
PasI CCCWGGG 1 cut(s) 2262
PciI ACATGT 4 cut(s) 9, 511, 1341, 1549
PcsI WCGNNNNNNNCGW 1 cut(s) 887
PctI GAATGC 1 cut(s) 2103
PdmI GAANNNNTTC 2 cut(s) 11, 1020
PfeI GAWTC 6 cut(s) 838, 853, 1144, 1169, 2082, 2368
Pfl23II CGTACG 1 cut(s) 2047
PinAI ACCGGT 1 cut(s) 436
PkrI GCNGC 3 cut(s) 56, 738, 1827
PleI GAGTC 4 cut(s) 266, 493, 901, 2104
PpsI GAGTC 4 cut(s) 266, 493, 901, 2104
Ppu21I YACGTR 1 cut(s) 2384
PpuMI RGGWCCY 1 cut(s) 1667
PscI ACATGT 4 cut(s) 9, 511, 1341, 1549
PshAI GACNNNNGTC 1 cut(s) 257
Psp5II RGGWCCY 1 cut(s) 1667
Psp6I CCWGG 3 cut(s) 213, 1536, 2261
PspEI GGTNACC 1 cut(s) 2095
PspFI CCCAGC 2 cut(s) 57, 338
PspGI CCWGG 3 cut(s) 213, 1536, 2261
PspLI CGTACG 1 cut(s) 2047
PspN4I GGNNCC 2 cut(s) 813, 1993
PspPI GGNCC 3 cut(s) 211, 987, 1667
PspPPI RGGWCCY 1 cut(s) 1667
PvuII CAGCTG 1 cut(s) 866
RsaI GTAC 6 cut(s) 219, 350, 613, 1899, 2049, 2454
RsaNI GTAC 6 cut(s) 218, 349, 612, 1898, 2048, 2453
RseI CAYNNNNRTG 2 cut(s) 2342, 2382
SatI GCNGC 3 cut(s) 55, 737, 1826
Sau96I GGNCC 3 cut(s) 211, 987, 1667
ScaI AGTACT 1 cut(s) 613
SchI GAGTC 4 cut(s) 267, 494, 901, 2104
ScrFI CCNGG 3 cut(s) 215, 1538, 2263
SduI GDGCHC 3 cut(s) 1279, 1996, 2251
SexAI ACCWGGT 1 cut(s) 213
SfaNI GCATC 8 cut(s) 192, 376, 928, 1100, 1935, 2315, 2380, 2674
SfcI CTRYAG 1 cut(s) 2114
SinI GGWCC 3 cut(s) 211, 987, 1667
SmiMI CAYNNNNRTG 2 cut(s) 2342, 2382
SmlI CTYRAG 2 cut(s) 248, 769
SmoI CTYRAG 2 cut(s) 248, 769
SphI GCATGC 1 cut(s) 1529
SsiI CCGC 7 cut(s) 239, 309, 737, 1044, 1497, 2101, 2607
SspI AATATT 3 cut(s) 900, 1862, 2629
SspMI CTAG 3 cut(s) 1610, 1949, 2150
StyD4I CCNGG 3 cut(s) 213, 1536, 2261
TaaI ACNGT 5 cut(s) 225, 475, 1079, 1102, 1985
TaiI ACGT 5 cut(s) 1121, 1365, 1461, 2034, 2386
TaqI TCGA 8 cut(s) 124, 286, 488, 881, 890, 1094, 1698, 2418
TatI WGTACW 1 cut(s) 611
TauI GCSGC 1 cut(s) 739
TfiI GAWTC 6 cut(s) 838, 853, 1144, 1169, 2082, 2368
TscAI CASTG 3 cut(s) 480, 1249, 2586
TseFI GTSAC 4 cut(s) 1073, 1466, 1490, 2095
TseI GCWGC 2 cut(s) 54, 1825
Tsp45I GTSAC 4 cut(s) 1073, 1466, 1490, 2095
TspDTI ATGAA 6 cut(s) 468, 537, 756, 2189, 2299, 2661
TspGWI ACGGA 4 cut(s) 366, 1895, 2065, 2354
TspRI CASTG 3 cut(s) 480, 1249, 2586
VpaK11BI GGWCC 3 cut(s) 211, 987, 1667
XagI CCTNNNNNAGG 1 cut(s) 2000
XapI RAATTY 5 cut(s) 291, 1220, 1438, 1798, 1971
XbaI TCTAGA 1 cut(s) 2149
XceI RCATGY 8 cut(s) 13, 515, 1345, 1419, 1529, 1553, 1956, 2577
XcmI CCANNNNNNNNNTGG 2 cut(s) 205, 686
XmnI GAANNNNTTC 2 cut(s) 11, 1020
XspI CTAG 3 cut(s) 1610, 1949, 2150
ZrmI AGTACT 1 cut(s) 613
Zsp2I ATGCAT 1 cut(s) 1455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.