Rroxscaffold_2G00083300

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000363
Physical Location & Seq
Forward (+)
5956776 .. 5959497
2722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00083300.1

Sequence Viewer

Length: 2697 bp
ATGGGTAACATCCTCACAGTCAACTTATCATGTGATGCCACTGTTTCTCGCTTCTTGGATTCAATTTTTCGACGAGCAGCATATGTTCGTAAGCTTGAAACAAACCTTCAACATCTGAATACTTCCTTCAATGAATTGAAGTGTTTAAAGGATGACGTAAAGAGAAGGGTTGCGGTTGCTGAGCAGCAGCATCACATGAAGCAGTTAGCCCAAGTACAGAACTGGATTTTAAAGGTGGAGGAATCTATAGAGGCTCAAGTAGAGGAAGCTGTCAACAATAGCACTCAACAAATAGAGAAACTGTGTTGTGGAGGCTGTTGTTCAAAGAACTACATGTCAAGCTACAAGTATGGGGGGAAAGTGGCTAAAATAGCAAGGGACTTTTTCCCAGAGGTTGCTGAAAAATTGCCCGAACCTCCAATCAGTGAAAGACCTCTGGAGCCAACTGTGGGGTTGGATTCGATGCTTGACAAGGTTTGGAGACAGATTGAAGATGAAAATGTGGCAGTAATTGGTTTATACGGGATGGGAGGGGTCGGCAAGACCAACCTCCTCACCCAAATCCATAACAAATTCCAACACACTGATTTCGATCTTGCAATCTGGATAGTAGTCTCCAAGGACCTCAGACTCGGAGCAATTCAAGATAGAATTGGAGAAAAGATTGGCTTTCCAAAAGACACATGGGAGCTTAAAGATCCAGATGCAAAAGCCGAAGAAATCTCACGCTTCTTGAGCAAAAAAAAGGTTGTGTTATTGTTGGATGATCTATGGGAGCGGGTTGATATAACCAAAATCGGAGTTCCATTTCCTGATCGATACAATAAGTCCAAGCTAGTCTTCACAACTCGTTCTGAGGATGTGTGCGGTATTATGGATGCTCAACAGAAGATTTATGTGGAGTGTTTGCCATGGGACAAAGCGTGGAACTTGTTTCAGGAGAAGGTTGGCAAAGACGCACTTCTTATTCATGCAGATATTCCTAAACTTGCTGAAGCTGTCGCAAAAGAGTGTGGTGGTTTGCCGCTTGCACTAATTACAGTTGGTCGGATGATGTCTTGCAAGAAGACACCCCAAGAATGGAATCATGCGATCAAAGTACTGAAGAAATTTAGCTTCGAGTTTTCAGGTATGGGAGACCAGGTGTTCCCTCTCTTGAAATTCAGTTACGATAATTTACCTAGTGAGAAAGTCAGATCATGCTTCTTGTACTGTGCTTTATTTCCTGAAGACTATCCCATACCTAAAGATGATTTAGCATGCTTTTGGATGTGTGAAAATATGTTAGATGAACATACTGATGTAGAAGAAGCACGAGATGAGAGCTACCATATCATAGGTATTCTTCTCAATGCATGTATGCTAGAAGAGAGTAAAGAAGGTTGTGTAAAAATGCATGATGTTGTTCGTGACATGGCATTGTGGTTGGCTTGTGATCCTAAGAAAGCAGATGAGAGTTTTCTTGTGCATGCAGGCGCTGATTTGATTGAAGCACCAATTGCCGAAAAATGGAAGAACTCGAAAAGGGTATCATTGATGGCTAACCACATCAAAGAATTGGTTGAAAAACCAAACTCTCCATATCTGTTGACTTTGTTTCTCACGGGAAATCATCTGAAGATTATTGTTGCAGGCTTCTTTGCCTCCATGTCTAAGCTGCTAGTTTTAGATCTGTCTTCAAATCATGATCTAACCCAACTGCCACCAGGAGTTTCGAGCTTGATTTCACTACAGCATCTCAATTTATCAAGCACTGGTATAAGAGAGTTGTCAATTGAGTTGAAGTGCTTAAAGAGGCTGACATATTTGAATTTTGAGTATACACCGAATCTTGATTCTCTTCCACCAACCATACTATCAAGTTTTTTGATGCTAAAAGTTCTGAGAATGGTACATTCTGGTACTTCTGATGAATGGAATCATTTTGATGATGGAAAAGTGATGGTAGAGGAATTGCATGGGTTGAAACAGCTGGACTACTTGACTCTGGACATTAGAAGTACCTCTTGTCTCCAAAACTTCGTCAGCAACAAATTAGTGAAGTGCTGCACTCGAGCTCTACACCTCATGGGTTATGACAGTCATTCGAGCTCTCTTGATATATCATCCGTGGAGACAAAACATCTTGAGCAACTTAACATTAGGGGTTATCCTAATGTGGAAGATATAATAGTTGACAATGATTGGGCAGGAGGACGAGCATGTAACAATCCTCAGAACTCAATGATGAGAATCCAGAGCTGCTTCCTTGGCCTTCAATACATAATGGTACAAGACTGTGTAAACCTCAAGGACTTGACACAGCTCTGTTTTGTTCCAAATCTCCTATCTGTTGACGTGAGGAGATGCTCTAGAATGGAGACAATAATCAATTTGAGTAAACTGGGTGGAGATGCAAATGTGGCAAAAAAATTTAACCTGTTTGCAAAAATGATGTATCTTCATCTCGATCACCTACCAGCGTTGGAGAGCGTATACAAAAATGCCTTGCCCTTTCCATGTCTAAAGTGGTTGCAAGTATATCAATGTCCAGTACTGAAGAAACTGCCACTCAACTCTAGCACTGCTCAAGGAGACGGATGCAATTTCATCCTAAGAGGAGAGCAGAAGTGGTGGGATGGGTTAGAGTGGGAGGACCAACTTGCACTAGATACTATTCGTCCTTGCTACGTGAGTTGGAATAGCCCCTACGATTAG

Protein Analysis

898

Amino Acids

102.05

Weight (kDa)

5.91

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 153 - 319 3.5e-45 NB-ARC domain
WHD_DRP PF23559 407 - 473 1.7e-08 Disease resistance protein Winged helix domain
LRR_8 PF13855 529 - 586 2e-07 Leucine rich repeat
LRR_14 PF23598 551 - 718 7.4e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 745 - 849 1.6e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12210 AT1G12210 AT1G12210 AT1G12220 AT1G12220 AT1G12220 AT1G12280 AT1G12280 AT1G12290 AT1G12290 AT1G12290 AT1G12290 AT1G15890 AT1G15890 AT1G51480 AT1G52660 AT1G52660 AT1G61180 AT1G61180 AT1G61190 AT1G61190 AT1G61190 AT1G61190 AT1G61300 AT1G61300 AT1G61310 AT3G15700 AT3G15700 AT4G10780 AT4G10780 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43740 AT5G43740 AT5G63020
fragaria_vesca FvH4_6g30050 FvH4_6g48220
malus_domestica MD09G1055800.v1.1 MD09G1198800.v1.1 MD17G1204800.v1.1 MD17G1204900.v1.1 MD17G1205000.v1.1 MD17G1205300.v1.1 MD17G1205700.v1.1 MD17G1205800.v1.1 MD17G1224100.v1.1
prunus_persica Prupe.3G054800_v2.0.a1 Prupe.3G054800_v2.0.a1 Prupe.3G080300_v2.0.a1 Prupe.3G080400_v2.0.a1 Prupe.3G080500_v2.0.a1 Prupe.3G080600_v2.0.a1 Prupe.3G080800_v2.0.a1 Prupe.3G083200_v2.0.a1 Prupe.3G099800_v2.0.a1 Prupe.3G099800_v2.0.a1
pyrus_communis pycom17g20860 pycom17g20910 pycom17g22790
rosa_chinensis RchiOBHm_Chr2g0136891 RchiOBHm_Chr2g0136901 RchiOBHm_Chr2g0167711 RchiOBHm_Chr2g0167721 RchiOBHm_Chr2g0167731 RchiOBHm_Chr4g0407611
rosa_laevigata RLG00000018943 RLG00000019278 RLG00000019282 RLG00000019587 RLG00000021748 RLG00000021749 RLG00000031057
rosa_multiflora Rmu_sc0002143.1_g000003 Rmu_sc0002782.1_g000001 Rmu_sc0023879.1_g000001 Rmu_sc0042171.1_g000001
rosa_roxburghii Rroxscaffold_2G00083300 Rroxscaffold_2G00107890 Rroxscaffold_2G00116680
rosa_rugosa Rorug02G0271700 Rorug02G0334900 Rorug02G0531800 Rorug02G0531800
rosa_samantha Rh2AG359900 Rh2AG385100 Rh2AG600500 Rh2AG600600 Rh2BG392200 Rh2BG611000 Rh2BG611100 Rh2BG611200 Rh2CG343100 Rh2CG343300 Rh2CG372300 Rh2CG581400 Rh2CG581500 Rh2DG382900 Rh2DG623700 Rh5AG027500 Rh5CG029900
rosa_wichuraiana Rw2G031490 Rw2G049850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 778
AccI GTMKAC 2 cut(s) 1820, 2475
AciI CCGC 4 cut(s) 173, 778, 867, 1025
AclWI GGATC 2 cut(s) 692, 1430
AcsI RAATTY 5 cut(s) 572, 1109, 1160, 1810, 2411
AcuI CTGAAG 5 cut(s) 1014, 1124, 1248, 1637, 2558
AfaI GTAC 8 cut(s) 216, 1101, 1211, 1893, 1903, 2002, 2271, 2535
AfiI CCNNNNNNNGG 3 cut(s) 449, 1080, 1509
AflIII ACRYGT 1 cut(s) 333
AjiI CACGTC 1 cut(s) 2338
AjnI CCWGG 2 cut(s) 1140, 1705
AjuI GAANNNNNNNTTGG 2 cut(s) 824, 856
Alw21I GWGCWC 2 cut(s) 2059, 2093
Alw26I GTCTC 7 cut(s) 475, 619, 1131, 2015, 2108, 2354, 2568
AlwI GGATC 2 cut(s) 692, 1430
AlwNI CAGNNNCTG 1 cut(s) 1478
Ama87I CYCGRG 1 cut(s) 2052
AoxI GGCC 1 cut(s) 2251
ApeKI GCWGC 6 cut(s) 77, 184, 187, 1657, 2046, 2241
ApoI RAATTY 5 cut(s) 572, 1109, 1160, 1810, 2411
AspLEI GCGC 1 cut(s) 1478
AspS9I GGNCC 2 cut(s) 622, 2635
AsuHPI GGTGA 2 cut(s) 547, 2444
AvaI CYCGRG 1 cut(s) 2052
AvaII GGWCC 2 cut(s) 622, 2635
BanII GRGCYC 2 cut(s) 2059, 2093
BauI CACGAG 1 cut(s) 1314
BbsI GAAGAC 4 cut(s) 832, 1073, 1236, 1668
Bbv12I GWGCWC 2 cut(s) 2059, 2093
BbvI GCAGC 6 cut(s) 89, 196, 199, 1644, 2033, 2228
BccI CCATC 5 cut(s) 520, 1531, 1925, 1936, 2612
BciT130I CCWGG 2 cut(s) 1142, 1707
BcoDI GTCTC 7 cut(s) 475, 619, 1131, 2015, 2108, 2354, 2568
BfaI CTAG 7 cut(s) 836, 1182, 1364, 1661, 2352, 2559, 2648
BfmI CTRYAG 2 cut(s) 246, 1730
BfoI RGCGCY 1 cut(s) 1479
BglII AGATCT 1 cut(s) 1669
BisI GCNGC 7 cut(s) 78, 185, 188, 1025, 1658, 2047, 2242
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 7 cut(s) 79, 186, 189, 1026, 1659, 2048, 2243
BmcAI AGTACT 2 cut(s) 1101, 2535
Bme1390I CCNGG 2 cut(s) 1142, 1707
Bme18I GGWCC 2 cut(s) 622, 2635
BmeT110I CYCGRG 1 cut(s) 2052
BmgBI CACGTC 1 cut(s) 2338
BmgT120I GGNCC 2 cut(s) 622, 2635
BmiI GGNNCC 1 cut(s) 441
BmrFI CCNGG 2 cut(s) 1142, 1707
BmrI ACTGGG 1 cut(s) 2393
BmuI ACTGGG 1 cut(s) 2393
BpiI GAAGAC 4 cut(s) 832, 1073, 1236, 1668
BpmI CTGGAG 1 cut(s) 458
Bpu1102I GCTNAGC 1 cut(s) 180
BpuEI CTTGAG 5 cut(s) 240, 754, 2147, 2273, 2553
Bsa29I ATCGAT 1 cut(s) 817
BsaAI YACGTR 1 cut(s) 2671
BsaBI GATNNNNATC 2 cut(s) 591, 2231
BsaI GGTCTC 1 cut(s) 1131
BsaJI CCNNGG 4 cut(s) 618, 911, 2109, 2248
BsaXI ACNNNNNCTCC 2 cut(s) 1128, 1158
Bsc4I CCNNNNNNNGG 3 cut(s) 449, 1080, 1509
Bse1I ACTGG 4 cut(s) 227, 1759, 2388, 2531
Bse8I GATNNNNATC 2 cut(s) 591, 2231
BseBI CCWGG 2 cut(s) 1142, 1707
BseCI ATCGAT 1 cut(s) 817
BseDI CCNNGG 4 cut(s) 618, 911, 2109, 2248
BseJI GATNNNNATC 2 cut(s) 591, 2231
BseLI CCNNNNNNNGG 3 cut(s) 449, 1080, 1509
BseMII CTCAG 5 cut(s) 171, 640, 846, 1874, 2228
BseNI ACTGG 4 cut(s) 227, 1759, 2388, 2531
BseRI GAGGAG 3 cut(s) 542, 2356, 2613
BseXI GCAGC 6 cut(s) 89, 196, 199, 1644, 2033, 2228
BsgI GTGCAG 1 cut(s) 2032
BshFI GGCC 1 cut(s) 2253
BshVI ATCGAT 1 cut(s) 817
BsiHKAI GWGCWC 2 cut(s) 2059, 2093
BsiHKCI CYCGRG 1 cut(s) 2052
BslFI GGGAC 2 cut(s) 392, 929
BslI CCNNNNNNNGG 3 cut(s) 449, 1080, 1509
BsmAI GTCTC 7 cut(s) 475, 619, 1131, 2015, 2108, 2354, 2568
BsmBI CGTCTC 1 cut(s) 2568
BsmFI GGGAC 2 cut(s) 392, 929
BsnI GGCC 1 cut(s) 2253
Bso31I GGTCTC 1 cut(s) 1131
BsoBI CYCGRG 1 cut(s) 2052
Bsp1286I GDGCHC 2 cut(s) 2059, 2093
Bsp1720I GCTNAGC 1 cut(s) 180
Bsp19I CCATGG 1 cut(s) 911
BspACI CCGC 4 cut(s) 173, 778, 867, 1025
BspANI GGCC 1 cut(s) 2253
BspCNI CTCAG 5 cut(s) 172, 639, 847, 1875, 2227
BspDI ATCGAT 1 cut(s) 817
BspHI TCATGA 1 cut(s) 1684
BspLI GGNNCC 1 cut(s) 441
BspPI GGATC 2 cut(s) 692, 1430
BspTNI GGTCTC 1 cut(s) 1131
BsrBI CCGCTC 1 cut(s) 778
BsrI ACTGG 4 cut(s) 227, 1759, 2388, 2531
BssECI CCNNGG 4 cut(s) 618, 911, 2109, 2248
BssNAI GTATAC 2 cut(s) 1821, 2476
BssSI CACGAG 1 cut(s) 1314
BssT1I CCWWGG 3 cut(s) 618, 911, 2248
Bst1107I GTATAC 2 cut(s) 1821, 2476
Bst2BI CACGAG 1 cut(s) 1314
Bst2UI CCWGG 2 cut(s) 1142, 1707
Bst4CI ACNGT 8 cut(s) 19, 43, 303, 448, 1042, 1214, 2081, 2279
Bst6I CTCTTC 2 cut(s) 1362, 1845
BstAPI GCANNNNNTGC 1 cut(s) 1499
BstBAI YACGTR 1 cut(s) 2671
BstC8I GCNNGC 5 cut(s) 1029, 1261, 1470, 1474, 1633
BstDEI CTNAG 8 cut(s) 180, 626, 855, 1440, 1653, 1883, 2214, 2594
BstDSI CCRYGG 2 cut(s) 911, 2109
BstH2I RGCGCY 1 cut(s) 1479
BstHHI GCGC 1 cut(s) 1478
BstMAI GTCTC 7 cut(s) 475, 619, 1131, 2015, 2108, 2354, 2568
BstMWI GCNNNNNNNGC 5 cut(s) 371, 735, 1499, 2250, 2402
BstNI CCWGG 2 cut(s) 1142, 1707
BstNSI RCATGY 5 cut(s) 337, 1263, 1359, 1472, 2205
BstSCI CCNGG 2 cut(s) 1140, 1705
BstSFI CTRYAG 2 cut(s) 246, 1730
BstV1I GCAGC 6 cut(s) 89, 196, 199, 1644, 2033, 2228
BstV2I GAAGAC 4 cut(s) 832, 1073, 1236, 1668
BstX2I RGATCY 2 cut(s) 697, 1669
BstYI RGATCY 2 cut(s) 697, 1669
BstZ17I GTATAC 2 cut(s) 1821, 2476
Bsu15I ATCGAT 1 cut(s) 817
BsuRI GGCC 1 cut(s) 2253
BsuTUI ATCGAT 1 cut(s) 817
BtgI CCRYGG 2 cut(s) 911, 2109
BtrI CACGTC 1 cut(s) 2338
BtsI GCAGTG 1 cut(s) 2562
BtsIMutI CAGTG 5 cut(s) 39, 430, 582, 1752, 2562
Cac8I GCNNGC 5 cut(s) 1029, 1261, 1470, 1474, 1633
CaiI CAGNNNCTG 1 cut(s) 1478
CciI TCATGA 1 cut(s) 1684
CfoI GCGC 1 cut(s) 1478
Cfr13I GGNCC 2 cut(s) 622, 2635
ClaI ATCGAT 1 cut(s) 817
CseI GACGC 1 cut(s) 965
CsiI ACCWGGT 1 cut(s) 1140
Csp6I GTAC 8 cut(s) 215, 1100, 1210, 1892, 1902, 2001, 2270, 2534
CviQI GTAC 8 cut(s) 215, 1100, 1210, 1892, 1902, 2001, 2270, 2534
DdeI CTNAG 8 cut(s) 180, 626, 855, 1440, 1653, 1883, 2214, 2594
DraI TTTAAA 2 cut(s) 147, 231
Eam1104I CTCTTC 2 cut(s) 1362, 1845
EarI CTCTTC 2 cut(s) 1362, 1845
Ecl136II GAGCTC 2 cut(s) 2057, 2091
Eco130I CCWWGG 3 cut(s) 618, 911, 2248
Eco24I GRGCYC 2 cut(s) 2059, 2093
Eco31I GGTCTC 1 cut(s) 1131
Eco47I GGWCC 2 cut(s) 622, 2635
Eco53kI GAGCTC 2 cut(s) 2057, 2091
Eco57I CTGAAG 5 cut(s) 1014, 1124, 1248, 1637, 2558
Eco88I CYCGRG 1 cut(s) 2052
EcoICRI GAGCTC 2 cut(s) 2057, 2091
EcoO109I RGGNCCY 1 cut(s) 622
EcoRII CCWGG 2 cut(s) 1140, 1705
EcoT14I CCWWGG 3 cut(s) 618, 911, 2248
EcoT22I ATGCAT 2 cut(s) 1357, 1398
EcoT38I GRGCYC 2 cut(s) 2059, 2093
ErhI CCWWGG 3 cut(s) 618, 911, 2248
Esp3I CGTCTC 1 cut(s) 2568
FalI AAGNNNNNCTT 8 cut(s) 653, 685, 824, 856, 945, 977, 1990, 2022
FaqI GGGAC 2 cut(s) 392, 929
FauI CCCGC 1 cut(s) 771
FauNDI CATATG 1 cut(s) 82
FblI GTMKAC 2 cut(s) 1820, 2475
Fnu4HI GCNGC 7 cut(s) 78, 185, 188, 1025, 1658, 2047, 2242
FriOI GRGCYC 2 cut(s) 2059, 2093
Fsp4HI GCNGC 7 cut(s) 78, 185, 188, 1025, 1658, 2047, 2242
FspBI CTAG 7 cut(s) 836, 1182, 1364, 1661, 2352, 2559, 2648
GlaI GCGC 1 cut(s) 1477
GluI GCNGC 7 cut(s) 78, 185, 188, 1025, 1658, 2047, 2242
GsuI CTGGAG 1 cut(s) 458
HaeII RGCGCY 1 cut(s) 1479
HaeIII GGCC 1 cut(s) 2253
HgaI GACGC 1 cut(s) 965
HhaI GCGC 1 cut(s) 1478
Hin6I GCGC 1 cut(s) 1476
HinP1I GCGC 1 cut(s) 1476
HincII GTYRAC 5 cut(s) 22, 274, 1590, 2176, 2335
HindII GTYRAC 5 cut(s) 22, 274, 1590, 2176, 2335
HindIII AAGCTT 1 cut(s) 92
HphI GGTGA 2 cut(s) 547, 2444
Hpy166II GTNNAC 9 cut(s) 22, 274, 1590, 1821, 2176, 2284, 2335, 2381, 2476
Hpy8I GTNNAC 9 cut(s) 22, 274, 1590, 1821, 2176, 2284, 2335, 2381, 2476
Hpy99I CGWCG 1 cut(s) 75
HpyAV CCTTC 6 cut(s) 116, 136, 159, 937, 1373, 2264
HpyCH4III ACNGT 8 cut(s) 19, 43, 303, 448, 1042, 1214, 2081, 2279
HpyCH4IV ACGT 3 cut(s) 156, 2337, 2670
HpyF10VI GCNNNNNNNGC 5 cut(s) 371, 735, 1499, 2250, 2402
HpyF3I CTNAG 8 cut(s) 180, 626, 855, 1440, 1653, 1883, 2214, 2594
HpySE526I ACGT 3 cut(s) 156, 2337, 2670
HspAI GCGC 1 cut(s) 1476
LmnI GCTCC 4 cut(s) 439, 635, 688, 775
Lsp1109I GCAGC 6 cut(s) 89, 196, 199, 1644, 2033, 2228
MabI ACCWGGT 1 cut(s) 1140
MaeI CTAG 7 cut(s) 836, 1182, 1364, 1661, 2352, 2559, 2648
MaeII ACGT 3 cut(s) 156, 2337, 2670
MaeIII GTNAC 4 cut(s) 5, 1166, 1409, 2204
MbiI CCGCTC 1 cut(s) 778
MfeI CAATTG 2 cut(s) 1497, 1773
MflI RGATCY 2 cut(s) 697, 1669
MhlI GDGCHC 2 cut(s) 2059, 2093
MlyI GAGTC 2 cut(s) 624, 1978
MmeI TCCRAC 6 cut(s) 435, 601, 741, 1028, 2445, 2657
Mph1103I ATGCAT 2 cut(s) 1357, 1398
MseI TTAA 6 cut(s) 146, 230, 693, 1790, 2136, 2415
MslI CAYNNNNRTG 2 cut(s) 1299, 1926
MspA1I CMGCKG 1 cut(s) 1972
MspR9I CCNGG 2 cut(s) 1142, 1707
MunI CAATTG 2 cut(s) 1497, 1773
MvaI CCWGG 2 cut(s) 1142, 1707
MwoI GCNNNNNNNGC 5 cut(s) 371, 735, 1499, 2250, 2402
NcoI CCATGG 1 cut(s) 911
NdeI CATATG 1 cut(s) 82
NlaIV GGNNCC 1 cut(s) 441
NmuCI GTSAC 1 cut(s) 1409
NsiI ATGCAT 2 cut(s) 1357, 1398
NspI RCATGY 5 cut(s) 337, 1263, 1359, 1472, 2205
PaeI GCATGC 2 cut(s) 1263, 1472
PaeR7I CTCGAG 1 cut(s) 2052
PagI TCATGA 1 cut(s) 1684
PciI ACATGT 1 cut(s) 333
PfeI GAWTC 8 cut(s) 59, 242, 458, 1084, 1828, 1835, 1918, 2232
PkrI GCNGC 7 cut(s) 79, 186, 189, 1026, 1659, 2048, 2243
PleI GAGTC 2 cut(s) 624, 1978
PpsI GAGTC 2 cut(s) 624, 1978
Ppu21I YACGTR 1 cut(s) 2671
PpuMI RGGWCCY 1 cut(s) 622
PscI ACATGT 1 cut(s) 333
Psp124BI GAGCTC 2 cut(s) 2059, 2093
Psp5II RGGWCCY 1 cut(s) 622
Psp6I CCWGG 2 cut(s) 1140, 1705
PspGI CCWGG 2 cut(s) 1140, 1705
PspN4I GGNNCC 1 cut(s) 441
PspPI GGNCC 2 cut(s) 622, 2635
PspPPI RGGWCCY 1 cut(s) 622
PspXI VCTCGAGB 1 cut(s) 2052
PstNI CAGNNNCTG 1 cut(s) 1478
PsuI RGATCY 2 cut(s) 697, 1669
PvuII CAGCTG 1 cut(s) 1972
RsaI GTAC 8 cut(s) 216, 1101, 1211, 1893, 1903, 2002, 2271, 2535
RsaNI GTAC 8 cut(s) 215, 1100, 1210, 1892, 1902, 2001, 2270, 2534
RseI CAYNNNNRTG 2 cut(s) 1299, 1926
SacI GAGCTC 2 cut(s) 2059, 2093
SaqAI TTAA 6 cut(s) 146, 230, 693, 1790, 2136, 2415
SatI GCNGC 7 cut(s) 78, 185, 188, 1025, 1658, 2047, 2242
Sau96I GGNCC 2 cut(s) 622, 2635
ScaI AGTACT 2 cut(s) 1101, 2535
SchI GAGTC 2 cut(s) 624, 1978
ScrFI CCNGG 2 cut(s) 1142, 1707
SduI GDGCHC 2 cut(s) 2059, 2093
SexAI ACCWGGT 1 cut(s) 1140
SfcI CTRYAG 2 cut(s) 246, 1730
Sfr274I CTCGAG 1 cut(s) 2052
SinI GGWCC 2 cut(s) 622, 2635
SlaI CTCGAG 1 cut(s) 2052
SmiMI CAYNNNNRTG 2 cut(s) 1299, 1926
SmlI CTYRAG 6 cut(s) 255, 733, 2052, 2126, 2288, 2568
SmoI CTYRAG 6 cut(s) 255, 733, 2052, 2126, 2288, 2568
SphI GCATGC 2 cut(s) 1263, 1472
SsiI CCGC 4 cut(s) 173, 778, 867, 1025
SspMI CTAG 7 cut(s) 836, 1182, 1364, 1661, 2352, 2559, 2648
SstI GAGCTC 2 cut(s) 2059, 2093
StyD4I CCNGG 2 cut(s) 1140, 1705
StyI CCWWGG 3 cut(s) 618, 911, 2248
TaaI ACNGT 8 cut(s) 19, 43, 303, 448, 1042, 1214, 2081, 2279
TaiI ACGT 3 cut(s) 159, 2340, 2673
TatI WGTACW 4 cut(s) 214, 1099, 1209, 2533
TauI GCSGC 1 cut(s) 1027
TfiI GAWTC 8 cut(s) 59, 242, 458, 1084, 1828, 1835, 1918, 2232
Tru1I TTAA 6 cut(s) 146, 230, 693, 1790, 2136, 2415
Tru9I TTAA 6 cut(s) 146, 230, 693, 1790, 2136, 2415
TscAI CASTG 5 cut(s) 46, 430, 589, 1759, 2569
TseFI GTSAC 1 cut(s) 1409
TseI GCWGC 6 cut(s) 77, 184, 187, 1657, 2046, 2241
Tsp45I GTSAC 1 cut(s) 1409
TspDTI ATGAA 8 cut(s) 147, 212, 510, 959, 1305, 1926, 2432, 2578
TspGWI ACGGA 2 cut(s) 2098, 2592
TspRI CASTG 5 cut(s) 46, 430, 589, 1759, 2569
VpaK11BI GGWCC 2 cut(s) 622, 2635
XapI RAATTY 5 cut(s) 572, 1109, 1160, 1810, 2411
XbaI TCTAGA 1 cut(s) 2351
XceI RCATGY 5 cut(s) 337, 1263, 1359, 1472, 2205
XcmI CCANNNNNNNNNTGG 2 cut(s) 681, 2505
XhoI CTCGAG 1 cut(s) 2052
XmiI GTMKAC 2 cut(s) 1820, 2475
XspI CTAG 7 cut(s) 836, 1182, 1364, 1661, 2352, 2559, 2648
ZrmI AGTACT 2 cut(s) 1101, 2535
Zsp2I ATGCAT 2 cut(s) 1357, 1398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.