Rmu_sc0002143.1_g000003

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002143.1
OG0000363
Physical Location & Seq
Reverse (-)
13142 .. 14792
1651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002143.1_g000003.1.cds

Sequence Viewer

Length: 1485 bp
atggatgctcaacagaagatttatgtggagtgtttgccatgggacaaagcgtggaacttgtttcaggagaaggttggcaaagacgcacttcttattcatgcagatattcctaaacttgctgaatctgtcgcaaaagagtgtgctggtatgggagacctggtgttccctctcttgaaattcagttacgataatttacctagtgagaaagtcagaccatgcttcttgtactgtgctttatttcctgaagactatcccatacctaaagatgatttagcatgcttttggatgtgtgaaaatatgttagatgaacatactgatctagaagaagcacgagatgagagctaccatatcataggtactcttctcaatgcatgtatgctagaagacagtaaagaaggttgtgcaaaaatgcatgatgttgttcgtgacatggcattgtggttggcttgtgatcctaagaaagcagaagagagttttcttgtgcgtgcaggcgctgatttgattgaagcaccaattgccgaaaaatggaagaactcgaaaagggtatcattgatggctaaccacatcaaagaattggttgaaaaaccaaactctccatatctgttgactttgtttctcaggagtaatcatctgaaaatgattattacaggcttctttgactctatgtctaatctgctggttttagatctgtctcgcaatatggatctaacccaactgccagtaggagtttcaagctgggtttcactacaacatctcaatttgtcacacactggtataagagagttgccgattgagttaaagtgcttaaagaggctgacatatttgaattttgagtatacactgaaacttgattctcttccaccaaccatactatcaagtttttcgatgcaaaaagttctgagaatggtaaattctggtacttctgatgatcgaaatcattttgatgatgaaaaagcgatggtagaggaattgcatggtttgaaacacctggactacttgactctggacattagaagtacctcttgtttccaaaacttcgtcagcaacaaattagtgaactctcttgatatatcatctgcggagacaaaacatcttgagcagattgagattagcggctatcctaatatggaagatataatagtggacattgattgggcaggaggacgagcatgtaacaatcctcggaactcaatgatgagaatccagagctgcttctttggccttcaatacataatggtaaacgcatgtgcagatctcaaggacttgtcacagctctgttttgttccaaatctccaatttgttttagtgttaagatgctctagaatggagacaataatcaatttgagtaaactgggtggagatgcaaatgtggcaaaagaatttaacctgtttgcgaaactgaagcgtcttgatttggcatttctaccagcgttggagagcgtatacaagactgccttgcccttaccatgtctaaagcagcactga

Protein Analysis

494

Amino Acids

56.21

Weight (kDa)

5.4

Isoelectric Point (pI)

49.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12210 AT1G12210 AT1G12210 AT1G12220 AT1G12220 AT1G12220 AT1G12280 AT1G12280 AT1G12290 AT1G12290 AT1G12290 AT1G12290 AT1G15890 AT1G15890 AT1G51480 AT1G52660 AT1G52660 AT1G61180 AT1G61180 AT1G61190 AT1G61190 AT1G61190 AT1G61190 AT1G61300 AT1G61300 AT1G61310 AT3G15700 AT3G15700 AT4G10780 AT4G10780 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43730 AT5G43740 AT5G43740 AT5G63020
fragaria_vesca FvH4_6g30050 FvH4_6g48220
malus_domestica MD09G1055800.v1.1 MD09G1198800.v1.1 MD17G1204800.v1.1 MD17G1204900.v1.1 MD17G1205000.v1.1 MD17G1205300.v1.1 MD17G1205700.v1.1 MD17G1205800.v1.1 MD17G1224100.v1.1
prunus_persica Prupe.3G054800_v2.0.a1 Prupe.3G054800_v2.0.a1 Prupe.3G080300_v2.0.a1 Prupe.3G080400_v2.0.a1 Prupe.3G080500_v2.0.a1 Prupe.3G080600_v2.0.a1 Prupe.3G080800_v2.0.a1 Prupe.3G083200_v2.0.a1 Prupe.3G099800_v2.0.a1 Prupe.3G099800_v2.0.a1
pyrus_communis pycom17g20860 pycom17g20910 pycom17g22790
rosa_chinensis RchiOBHm_Chr2g0136891 RchiOBHm_Chr2g0136901 RchiOBHm_Chr2g0167711 RchiOBHm_Chr2g0167721 RchiOBHm_Chr2g0167731 RchiOBHm_Chr4g0407611
rosa_laevigata RLG00000018943 RLG00000019278 RLG00000019282 RLG00000019587 RLG00000021748 RLG00000021749 RLG00000031057
rosa_multiflora Rmu_sc0002143.1_g000003 Rmu_sc0002782.1_g000001 Rmu_sc0023879.1_g000001 Rmu_sc0042171.1_g000001
rosa_roxburghii Rroxscaffold_2G00083300 Rroxscaffold_2G00107890 Rroxscaffold_2G00116680
rosa_rugosa Rorug02G0271700 Rorug02G0334900 Rorug02G0531800 Rorug02G0531800
rosa_samantha Rh2AG359900 Rh2AG385100 Rh2AG600500 Rh2AG600600 Rh2BG392200 Rh2BG611000 Rh2BG611100 Rh2BG611200 Rh2CG343100 Rh2CG343300 Rh2CG372300 Rh2CG581400 Rh2CG581500 Rh2DG382900 Rh2DG623700 Rh5AG027500 Rh5CG029900
rosa_wichuraiana Rw2G031490 Rw2G049850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 836, 1443
AciI CCGC 2 cut(s) 1079, 1113
AclWI GGATC 2 cut(s) 446, 711
AcsI RAATTY 4 cut(s) 176, 826, 910, 1379
AcuI CTGAAG 2 cut(s) 264, 1421
AfaI GTAC 4 cut(s) 227, 358, 919, 1018
AfiI CCNNNNNNNGG 1 cut(s) 525
AgsI TTSAA 7 cut(s) 175, 506, 581, 732, 826, 982, 1226
AhdI GACNNNNNGTC 1 cut(s) 664
AjnI CCWGG 2 cut(s) 156, 987
AluBI AGCT 4 cut(s) 342, 735, 1209, 1273
AluI AGCT 4 cut(s) 342, 735, 1209, 1273
Alw26I GTCTC 4 cut(s) 147, 696, 1076, 1322
AlwI GGATC 2 cut(s) 446, 711
AlwNI CAGNNNCTG 1 cut(s) 494
AoxI GGCC 1 cut(s) 1219
ApeKI GCWGC 2 cut(s) 1209, 1477
ApoI RAATTY 4 cut(s) 176, 826, 910, 1379
AspLEI GCGC 1 cut(s) 494
BauI CACGAG 1 cut(s) 330
BbsI GAAGAC 2 cut(s) 252, 390
BbvI GCAGC 1 cut(s) 1196
BccI CCATC 2 cut(s) 547, 952
BciT130I CCWGG 2 cut(s) 158, 989
BcoDI GTCTC 4 cut(s) 147, 696, 1076, 1322
BfaI CTAG 4 cut(s) 198, 320, 380, 1320
BfoI RGCGCY 1 cut(s) 495
BglII AGATCT 2 cut(s) 685, 1252
BisI GCNGC 3 cut(s) 1114, 1210, 1478
BlsI GCNGC 3 cut(s) 1115, 1211, 1479
Bme1390I CCNGG 2 cut(s) 158, 989
BmeRI GACNNNNNGTC 1 cut(s) 664
BmrFI CCNGG 2 cut(s) 158, 989
BmrI ACTGGG 1 cut(s) 1361
BmsI GCATC 3 cut(s) 876, 1304, 1351
BmuI ACTGGG 1 cut(s) 1361
BpiI GAAGAC 2 cut(s) 252, 390
BpuEI CTTGAG 2 cut(s) 1115, 1241
BsaBI GATNNNNATC 2 cut(s) 933, 1199
BsaI GGTCTC 1 cut(s) 147
BsaJI CCNNGG 2 cut(s) 38, 1181
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bsc4I CCNNNNNNNGG 1 cut(s) 525
Bse1I ACTGG 3 cut(s) 719, 775, 1356
Bse8I GATNNNNATC 2 cut(s) 933, 1199
BseBI CCWGG 2 cut(s) 158, 989
BseDI CCNNGG 2 cut(s) 38, 1181
BseGI GGATG 2 cut(s) 10, 291
BseJI GATNNNNATC 2 cut(s) 933, 1199
BseLI CCNNNNNNNGG 1 cut(s) 525
BseMII CTCAG 2 cut(s) 631, 890
BseNI ACTGG 3 cut(s) 719, 775, 1356
BseXI GCAGC 1 cut(s) 1196
BseYI CCCAGC 1 cut(s) 735
BsgI GTGCAG 2 cut(s) 507, 1269
BshFI GGCC 1 cut(s) 1221
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 1 cut(s) 525
BsmAI GTCTC 4 cut(s) 147, 696, 1076, 1322
BsmFI GGGAC 1 cut(s) 56
BsnI GGCC 1 cut(s) 1221
Bso31I GGTCTC 1 cut(s) 147
Bsp143I GATC 6 cut(s) 316, 451, 685, 703, 928, 1252
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 2 cut(s) 1079, 1113
BspANI GGCC 1 cut(s) 1221
BspCNI CTCAG 2 cut(s) 630, 891
BspPI GGATC 2 cut(s) 446, 711
BspTNI GGTCTC 1 cut(s) 147
BsrI ACTGG 3 cut(s) 719, 775, 1356
BssECI CCNNGG 2 cut(s) 38, 1181
BssMI GATC 6 cut(s) 316, 451, 685, 703, 928, 1252
BssNAI GTATAC 2 cut(s) 837, 1444
BssSI CACGAG 1 cut(s) 330
BssT1I CCWWGG 1 cut(s) 38
Bst1107I GTATAC 2 cut(s) 837, 1444
Bst2BI CACGAG 1 cut(s) 330
Bst2UI CCWGG 2 cut(s) 158, 989
Bst4CI ACNGT 2 cut(s) 230, 389
Bst6I CTCTTC 3 cut(s) 366, 462, 861
BstAPI GCANNNNNTGC 1 cut(s) 515
BstC8I GCNNGC 3 cut(s) 277, 486, 490
BstDEI CTNAG 3 cut(s) 456, 617, 899
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 2 cut(s) 10, 291
BstH2I RGCGCY 1 cut(s) 495
BstHHI GCGC 1 cut(s) 494
BstKTI GATC 6 cut(s) 319, 454, 688, 706, 931, 1255
BstMAI GTCTC 4 cut(s) 147, 696, 1076, 1322
BstMBI GATC 6 cut(s) 316, 451, 685, 703, 928, 1252
BstMWI GCNNNNNNNGC 3 cut(s) 515, 1218, 1370
BstNI CCWGG 2 cut(s) 158, 989
BstNSI RCATGY 4 cut(s) 279, 375, 1173, 1248
BstSCI CCNGG 2 cut(s) 156, 987
BstV1I GCAGC 1 cut(s) 1196
BstV2I GAAGAC 2 cut(s) 252, 390
BstX2I RGATCY 3 cut(s) 685, 703, 1252
BstYI RGATCY 3 cut(s) 685, 703, 1252
BstZ17I GTATAC 2 cut(s) 837, 1444
BsuRI GGCC 1 cut(s) 1221
BtgI CCRYGG 1 cut(s) 38
BtgZI GCGATG 1 cut(s) 971
BtsCI GGATG 2 cut(s) 10, 291
BtsIMutI CAGTG 3 cut(s) 768, 839, 1480
Cac8I GCNNGC 3 cut(s) 277, 486, 490
CaiI CAGNNNCTG 1 cut(s) 494
CfoI GCGC 1 cut(s) 494
CseI GACGC 2 cut(s) 92, 1394
CsiI ACCWGGT 1 cut(s) 156
Csp6I GTAC 4 cut(s) 226, 357, 918, 1017
CviQI GTAC 4 cut(s) 226, 357, 918, 1017
DdeI CTNAG 3 cut(s) 456, 617, 899
DpnI GATC 6 cut(s) 318, 453, 687, 705, 930, 1254
DpnII GATC 6 cut(s) 316, 451, 685, 703, 928, 1252
DriI GACNNNNNGTC 1 cut(s) 664
Eam1104I CTCTTC 3 cut(s) 366, 462, 861
Eam1105I GACNNNNNGTC 1 cut(s) 664
EarI CTCTTC 3 cut(s) 366, 462, 861
Eco130I CCWWGG 1 cut(s) 38
Eco31I GGTCTC 1 cut(s) 147
Eco57I CTGAAG 2 cut(s) 264, 1421
EcoRII CCWGG 2 cut(s) 156, 987
EcoT14I CCWWGG 1 cut(s) 38
EcoT22I ATGCAT 2 cut(s) 373, 414
ErhI CCWWGG 1 cut(s) 38
FalI AAGNNNNNCTT 6 cut(s) 72, 104, 1006, 1038, 1439, 1471
FaqI GGGAC 1 cut(s) 56
FblI GTMKAC 2 cut(s) 836, 1443
Fnu4HI GCNGC 3 cut(s) 1114, 1210, 1478
FokI GGATG 2 cut(s) 17, 298
Fsp4HI GCNGC 3 cut(s) 1114, 1210, 1478
FspBI CTAG 4 cut(s) 198, 320, 380, 1320
GlaI GCGC 1 cut(s) 493
GluI GCNGC 3 cut(s) 1114, 1210, 1478
GsaI CCCAGC 1 cut(s) 739
HaeII RGCGCY 1 cut(s) 495
HaeIII GGCC 1 cut(s) 1221
HgaI GACGC 2 cut(s) 92, 1394
HhaI GCGC 1 cut(s) 494
Hin6I GCGC 1 cut(s) 492
HinP1I GCGC 1 cut(s) 492
HincII GTYRAC 1 cut(s) 606
HindII GTYRAC 1 cut(s) 606
HinfI GANTC 5 cut(s) 122, 659, 851, 1000, 1200
Hpy166II GTNNAC 7 cut(s) 606, 837, 1057, 1144, 1240, 1349, 1444
Hpy188I TCNGA 5 cut(s) 212, 633, 900, 925, 1185
Hpy8I GTNNAC 7 cut(s) 606, 837, 1057, 1144, 1240, 1349, 1444
HpyAV CCTTC 3 cut(s) 64, 389, 1232
HpyCH4III ACNGT 2 cut(s) 230, 389
HpyCH4V TGCA 9 cut(s) 101, 371, 404, 412, 488, 889, 973, 1250, 1364
HpyF10VI GCNNNNNNNGC 3 cut(s) 515, 1218, 1370
HpyF3I CTNAG 3 cut(s) 456, 617, 899
HspAI GCGC 1 cut(s) 492
Kzo9I GATC 6 cut(s) 316, 451, 685, 703, 928, 1252
Lsp1109I GCAGC 1 cut(s) 1196
LweI GCATC 3 cut(s) 876, 1304, 1351
MabI ACCWGGT 1 cut(s) 156
MaeI CTAG 4 cut(s) 198, 320, 380, 1320
MaeIII GTNAC 5 cut(s) 182, 425, 762, 1172, 1266
MalI GATC 6 cut(s) 318, 453, 687, 705, 930, 1254
MboI GATC 6 cut(s) 316, 451, 685, 703, 928, 1252
MboII GAAGA 9 cut(s) 28, 257, 335, 353, 395, 479, 541, 848, 1142
MfeI CAATTG 1 cut(s) 513
MflI RGATCY 3 cut(s) 685, 703, 1252
MlyI GAGTC 2 cut(s) 653, 994
MmeI TCCRAC 1 cut(s) 1413
MnlI CCTC 6 cut(s) 177, 804, 958, 1030, 1154, 1191
Mph1103I ATGCAT 2 cut(s) 373, 414
MseI TTAA 4 cut(s) 797, 806, 1310, 1383
MslI CAYNNNNRTG 1 cut(s) 942
MspR9I CCNGG 2 cut(s) 158, 989
MunI CAATTG 1 cut(s) 513
MvaI CCWGG 2 cut(s) 158, 989
MwoI GCNNNNNNNGC 3 cut(s) 515, 1218, 1370
NcoI CCATGG 1 cut(s) 38
NdeII GATC 6 cut(s) 316, 451, 685, 703, 928, 1252
NmuCI GTSAC 3 cut(s) 425, 762, 1266
NsiI ATGCAT 2 cut(s) 373, 414
NspI RCATGY 4 cut(s) 279, 375, 1173, 1248
PaeI GCATGC 1 cut(s) 279
PfeI GAWTC 3 cut(s) 122, 851, 1200
PkrI GCNGC 3 cut(s) 1115, 1211, 1479
PleI GAGTC 2 cut(s) 653, 994
PpsI GAGTC 2 cut(s) 653, 994
Psp6I CCWGG 2 cut(s) 156, 987
PspFI CCCAGC 1 cut(s) 735
PspGI CCWGG 2 cut(s) 156, 987
PstNI CAGNNNCTG 1 cut(s) 494
PsuI RGATCY 3 cut(s) 685, 703, 1252
RsaI GTAC 4 cut(s) 227, 358, 919, 1018
RsaNI GTAC 4 cut(s) 226, 357, 918, 1017
RseI CAYNNNNRTG 1 cut(s) 942
SaqAI TTAA 4 cut(s) 797, 806, 1310, 1383
SatI GCNGC 3 cut(s) 1114, 1210, 1478
Sau3AI GATC 6 cut(s) 316, 451, 685, 703, 928, 1252
SchI GAGTC 2 cut(s) 653, 994
ScrFI CCNGG 2 cut(s) 158, 989
SexAI ACCWGGT 1 cut(s) 156
SfaNI GCATC 3 cut(s) 876, 1304, 1351
SmiMI CAYNNNNRTG 1 cut(s) 942
SmlI CTYRAG 2 cut(s) 1094, 1256
SmoI CTYRAG 2 cut(s) 1094, 1256
SphI GCATGC 1 cut(s) 279
SsiI CCGC 2 cut(s) 1079, 1113
SspMI CTAG 4 cut(s) 198, 320, 380, 1320
StyD4I CCNGG 2 cut(s) 156, 987
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 2 cut(s) 230, 389
TaqI TCGA 3 cut(s) 536, 884, 931
TatI WGTACW 1 cut(s) 225
TauI GCSGC 1 cut(s) 1116
TfiI GAWTC 3 cut(s) 122, 851, 1200
Tru1I TTAA 4 cut(s) 797, 806, 1310, 1383
Tru9I TTAA 4 cut(s) 797, 806, 1310, 1383
TscAI CASTG 2 cut(s) 775, 846
TseFI GTSAC 3 cut(s) 425, 762, 1266
TseI GCWGC 2 cut(s) 1209, 1477
Tsp45I GTSAC 3 cut(s) 425, 762, 1266
TspDTI ATGAA 3 cut(s) 86, 321, 963
TspRI CASTG 2 cut(s) 775, 846
XapI RAATTY 4 cut(s) 176, 826, 910, 1379
XbaI TCTAGA 2 cut(s) 319, 1319
XceI RCATGY 4 cut(s) 279, 375, 1173, 1248
XmiI GTMKAC 2 cut(s) 836, 1443
XspI CTAG 4 cut(s) 198, 320, 380, 1320
Zsp2I ATGCAT 2 cut(s) 373, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.