MD03G1043800.v1.1

FH protein interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000859
Physical Location & Seq
Reverse (-)
3459728 .. 3464309
4582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1043800.v1.1.491

Sequence Viewer

Length: 1722 bp
ATGGCAGAGGACTGTGATTCTTCATCCTTGGTTCACCTCAATGTGGGAGGAAAGAAGTTTTCCACCACCGTCGGTACTCTAACTCAGCGTCAACCCGACTCCATGCTTGCTGCAATGTTTAGCGGTCGACACACTTTGCGCCAAGATAAGAAGGGATATGTTTTTATCGATAGGGATGGCGAACATTTTCGGCACATTCTTAACTGGTTAAGAGACGGTGAGGTTCCTAAATTAGAAGCTTCCCAATATGCAGAACTTGTTAAGGAGGCAAAGTACTATCAACTACTAGGGTTAGTTGATGGAATACCTGATGTCCTGAACAGTAAGGAAGATAAGAAGTTGGATGCAGATTTTACCCGTGCCGAGGTTATCGACTACATATATACAAAGAAGTGGTCTAGAGGCGTGGGTGGTTGTACGTATTATTTTCGAGGGGTTAATCTTTCTGGCCGTAATCTTTCAAAACTGGACCTGTCACATGTCAATTTCGGCTATGCATGTCTGAGAAACGTGTGTTTCTCAGGTGCAAATATTCAAGGGTCTAATTTCAACTACGCTGATGCTGAAGGTGCTACCTTTGACAGTGCAAATTTGCGTAGTTCTACTTTTTATCATGCAAATCTTCGAGGAGCTTCTTTTGTGGGTGCTAAAAACTACGATCTCGTTGGGGCATCTTGCAACCAGCTGTACCCAGAAGTACAGCCGCTGTTTCTTCATTTCCTTCTCCGTTTTGCTCTCCCAAACTCAAATCTTCACATCTCCTTCATTTGGTCACCGTTTTCGACAAGTCTTATATCAAAGTCTAAGCCCTCGTCGAGCCCAACGCGTTTTGTAACCTCGATTCCCCCCCCCCCCCCCCCGCCCAAAAAAAAAAAAACCACCACACAAGGCAGAAGAAAGGCGAGAAAGGGAGCAAATCTCGCCATAAAACTTCAAGTCCGGCCAAACTCATCGGAACTCGACCTTAAGCCAAGAGAGAAGTTTAATCCTCTTCTCTTCGGCTTCATTTCTACATTTGTGGTCATCTCTCAAAGTTTCCAAGCAATGCCAGAGGTCTACGATTCTTCATCCTTGGTTAACCTCAATGTGGCAGGAAAGAAGTTTTGCACCACCGTCGGTACTCTAACTCAGCGTCAACCCGACTCCATGCTTGCTGCAATGTTCAGCGGTCGACACACTTTGCGCAAAGATAAGAAGGGAGACGGCGAGATTCCTAAATTAGAAGATTCTCAATATGCAGAACTTGTTAAGGAGGCAAAATACTATCTACTATTTGATTTAGTTGATGGAATACTTGATGTCCTTGATAGTAAGGAAGATACTGCGTCGTATGCAGATTTTACACGCACCGACGTTATCAAGTACATACATTCTCAGAAGTCGTGTAGATGCTCGTCTAATAAGAATTATCTTCAAGGGGTTAATCTTTCTGGCCTTAATATTTCAAAATTGGACCTGTCAGAAGTTAACTTCAGCTCTGCATGTTTGAGAAATGTGAGTATTTCAGGTGCAAATCTTCAGTCTGCTAATTTCAAACATGTGGATGCCGAAGGTGCTACTTTTGACGATGCCAATTTGTGTGGTTCTACTTTTTATGGTGCAAATCTACGAGGAGCGTCTTTTGTGGGTGCAAATCTACGAGGAGCGTCTTTAATGGGTGCAAAAAACTTCGATCTCTACGATCTCTCTGGGGCATCTGGCGTCGAATATTGTAGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB_2 Pentapeptide

Protein Analysis

574

Amino Acids

63.3

Weight (kDa)

8.93

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB_2 PF02214 11 - 98 1.3e-19 BTB/POZ domain
Pentapeptide PF00805 143 - 180 1.2e-06 Pentapeptide repeats (8 copies)
Pentapeptide_4 PF13599 143 - 213 3.6e-07 Pentapeptide repeat region
Pentapeptide PF00805 176 - 213 1e-06 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 471 - 507 3.4e-06 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 504 - 541 1.2e-08 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 521 - 554 1.8e-08 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 531 - 564 5.1e-06 Pentapeptide repeats (8 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1184
AccI GTMKAC 3 cut(s) 127, 1056, 1171
AccII CGCG 1 cut(s) 826
AciI CCGC 4 cut(s) 123, 704, 860, 1167
AcoI YGGCCR 2 cut(s) 448, 941
AcsI RAATTY 1 cut(s) 589
AcuI CTGAAG 3 cut(s) 585, 1456, 1502
AcyI GRCGYC 1 cut(s) 1701
AfaI GTAC 7 cut(s) 76, 275, 418, 689, 699, 1120, 1364
AfiI CCNNNNNNNGG 4 cut(s) 43, 364, 768, 1087
AflII CTTAAG 1 cut(s) 965
AflIII ACRYGT 4 cut(s) 478, 510, 824, 1537
AgsI TTSAA 7 cut(s) 462, 536, 550, 935, 1415, 1446, 1534
AluBI AGCT 5 cut(s) 239, 632, 685, 1476, 1716
AluI AGCT 5 cut(s) 239, 632, 685, 1476, 1716
Alw26I GTCTC 2 cut(s) 207, 1194
AlwNI CAGNNNCTG 1 cut(s) 706
AoxI GGCC 3 cut(s) 448, 941, 1432
ApeKI GCWGC 2 cut(s) 110, 1154
ApoI RAATTY 1 cut(s) 589
ArsI GACNNNNNNTTYG 4 cut(s) 380, 412, 1505, 1537
Asp700I GAANNNNTTC 1 cut(s) 186
AspLEI GCGC 2 cut(s) 141, 1185
AspS9I GGNCC 2 cut(s) 469, 1453
AsuHPI GGTGA 3 cut(s) 26, 230, 765
AvaII GGWCC 2 cut(s) 469, 1453
BanII GRGCYC 1 cut(s) 821
BbvI GCAGC 2 cut(s) 97, 1141
BccI CCATC 3 cut(s) 170, 293, 1280
BceAI ACGGC 2 cut(s) 435, 1219
BcgI CGANNNNNNTGC 2 cut(s) 1096, 1130
BcoDI GTCTC 2 cut(s) 207, 1194
BfaI CTAG 2 cut(s) 287, 399
BfrI CTTAAG 1 cut(s) 965
BisI GCNGC 3 cut(s) 111, 704, 1155
BlsI GCNGC 3 cut(s) 112, 705, 1156
BmcAI AGTACT 1 cut(s) 275
Bme18I GGWCC 2 cut(s) 469, 1453
BmgT120I GGNCC 2 cut(s) 469, 1453
BmiI GGNNCC 1 cut(s) 225
BmsI GCATC 7 cut(s) 334, 550, 680, 1379, 1534, 1558, 1703
BplI GAGNNNNNCTC 2 cut(s) 903, 935
Bsa29I ATCGAT 1 cut(s) 168
BsaAI YACGTR 1 cut(s) 420
BsaHI GRCGYC 1 cut(s) 1701
BsaJI CCNNGG 3 cut(s) 27, 363, 1071
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 364, 768, 1087
Bse1I ACTGG 2 cut(s) 209, 471
Bse3DI GCAATG 3 cut(s) 120, 1050, 1164
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 3 cut(s) 27, 363, 1071
BseGI GGATG 5 cut(s) 23, 181, 349, 1067, 1549
BseLI CCNNNNNNNGG 4 cut(s) 43, 364, 768, 1087
BseMI GCAATG 3 cut(s) 120, 1050, 1164
BseMII CTCAG 5 cut(s) 98, 494, 534, 1142, 1388
BseNI ACTGG 2 cut(s) 209, 471
BseRI GAGGAG 3 cut(s) 642, 1626, 1656
BseXI GCAGC 2 cut(s) 97, 1141
Bsh1236I CGCG 1 cut(s) 826
Bsh1285I CGRYCG 2 cut(s) 127, 1171
BshFI GGCC 3 cut(s) 450, 943, 1434
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 2 cut(s) 127, 1171
BsiSI CCGG 1 cut(s) 940
BslI CCNNNNNNNGG 4 cut(s) 43, 364, 768, 1087
BsmAI GTCTC 2 cut(s) 207, 1194
BsmBI CGTCTC 2 cut(s) 207, 1194
BsnI GGCC 3 cut(s) 450, 943, 1434
Bsp1286I GDGCHC 1 cut(s) 821
Bsp143I GATC 3 cut(s) 658, 1672, 1681
BspACI CCGC 4 cut(s) 123, 704, 860, 1167
BspANI GGCC 3 cut(s) 450, 943, 1434
BspCNI CTCAG 5 cut(s) 97, 495, 533, 1141, 1387
BspDI ATCGAT 1 cut(s) 168
BspFNI CGCG 1 cut(s) 826
BspLI GGNNCC 1 cut(s) 225
BspTI CTTAAG 1 cut(s) 965
BsrDI GCAATG 3 cut(s) 120, 1050, 1164
BsrI ACTGG 2 cut(s) 209, 471
BssECI CCNNGG 3 cut(s) 27, 363, 1071
BssMI GATC 3 cut(s) 658, 1672, 1681
BssNI GRCGYC 1 cut(s) 1701
BssT1I CCWWGG 2 cut(s) 27, 1071
Bst4CI ACNGT 7 cut(s) 14, 70, 218, 323, 584, 777, 1114
Bst6I CTCTTC 2 cut(s) 996, 1001
BstACI GRCGYC 1 cut(s) 1701
BstAFI CTTAAG 1 cut(s) 965
BstBAI YACGTR 1 cut(s) 420
BstC8I GCNNGC 2 cut(s) 108, 1152
BstDEI CTNAG 6 cut(s) 84, 503, 520, 804, 1128, 1374
BstEII GGTNACC 1 cut(s) 771
BstF5I GGATG 5 cut(s) 23, 181, 349, 1067, 1549
BstFNI CGCG 1 cut(s) 826
BstHHI GCGC 2 cut(s) 141, 1185
BstKTI GATC 3 cut(s) 661, 1675, 1684
BstMAI GTCTC 2 cut(s) 207, 1194
BstMBI GATC 3 cut(s) 658, 1672, 1681
BstMCI CGRYCG 2 cut(s) 127, 1171
BstMWI GCNNNNNNNGC 4 cut(s) 569, 920, 1331, 1553
BstNSI RCATGY 4 cut(s) 482, 501, 1485, 1541
BstPI GGTNACC 1 cut(s) 771
BstSNI TACGTA 1 cut(s) 420
BstUI CGCG 1 cut(s) 826
BstV1I GCAGC 2 cut(s) 97, 1141
Bsu15I ATCGAT 1 cut(s) 168
BsuRI GGCC 3 cut(s) 450, 943, 1434
BsuTUI ATCGAT 1 cut(s) 168
BtsCI GGATG 5 cut(s) 23, 181, 349, 1067, 1549
BtsIMutI CAGTG 1 cut(s) 589
Cac8I GCNNGC 2 cut(s) 108, 1152
CaiI CAGNNNCTG 1 cut(s) 706
CfoI GCGC 2 cut(s) 141, 1185
Cfr13I GGNCC 2 cut(s) 469, 1453
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 6 cut(s) 77, 1121, 1314, 1605, 1635, 1690
Csp6I GTAC 7 cut(s) 75, 274, 417, 688, 698, 1119, 1363
CspCI CAANNNNNGTGG 2 cut(s) 1561, 1596
CviAII CATG 7 cut(s) 103, 479, 498, 614, 1147, 1482, 1538
CviQI GTAC 7 cut(s) 75, 274, 417, 688, 698, 1119, 1363
DdeI CTNAG 6 cut(s) 84, 503, 520, 804, 1128, 1374
DpnI GATC 3 cut(s) 660, 1674, 1683
DpnII GATC 3 cut(s) 658, 1672, 1681
EaeI YGGCCR 2 cut(s) 448, 941
Eam1104I CTCTTC 2 cut(s) 996, 1001
EarI CTCTTC 2 cut(s) 996, 1001
Eco105I TACGTA 1 cut(s) 420
Eco130I CCWWGG 2 cut(s) 27, 1071
Eco24I GRGCYC 1 cut(s) 821
Eco47I GGWCC 2 cut(s) 469, 1453
Eco57I CTGAAG 3 cut(s) 585, 1456, 1502
Eco91I GGTNACC 1 cut(s) 771
EcoO65I GGTNACC 1 cut(s) 771
EcoT14I CCWWGG 2 cut(s) 27, 1071
EcoT22I ATGCAT 1 cut(s) 499
EcoT38I GRGCYC 1 cut(s) 821
ErhI CCWWGG 2 cut(s) 27, 1071
Esp3I CGTCTC 2 cut(s) 207, 1194
FaeI CATG 7 cut(s) 106, 482, 501, 617, 1150, 1485, 1541
FatI CATG 7 cut(s) 102, 478, 497, 613, 1146, 1481, 1537
FauI CCCGC 1 cut(s) 867
FblI GTMKAC 3 cut(s) 127, 1056, 1171
Fnu4HI GCNGC 3 cut(s) 111, 704, 1155
FokI GGATG 5 cut(s) 10, 188, 356, 1054, 1556
FriOI GRGCYC 1 cut(s) 821
Fsp4HI GCNGC 3 cut(s) 111, 704, 1155
FspBI CTAG 2 cut(s) 287, 399
FspI TGCGCA 1 cut(s) 1184
GlaI GCGC 2 cut(s) 140, 1184
GluI GCNGC 3 cut(s) 111, 704, 1155
HaeIII GGCC 3 cut(s) 450, 943, 1434
HapII CCGG 1 cut(s) 940
HgaI GACGC 6 cut(s) 77, 1121, 1314, 1605, 1635, 1690
HhaI GCGC 2 cut(s) 141, 1185
Hin1I GRCGYC 1 cut(s) 1701
Hin1II CATG 7 cut(s) 106, 482, 501, 617, 1150, 1485, 1541
Hin6I GCGC 2 cut(s) 139, 1183
HinP1I GCGC 2 cut(s) 139, 1183
HincII GTYRAC 6 cut(s) 92, 128, 1078, 1136, 1172, 1468
HindII GTYRAC 6 cut(s) 92, 128, 1078, 1136, 1172, 1468
HindIII AAGCTT 1 cut(s) 237
HinfI GANTC 7 cut(s) 17, 98, 841, 1061, 1142, 1210, 1226
HpaI GTTAAC 2 cut(s) 1078, 1468
HpaII CCGG 1 cut(s) 940
HphI GGTGA 3 cut(s) 26, 230, 765
Hpy166II GTNNAC 8 cut(s) 34, 92, 128, 1057, 1078, 1136, 1172, 1468
Hpy188I TCNGA 5 cut(s) 504, 955, 1377, 1462, 1721
Hpy188III TCNNGA 2 cut(s) 316, 399
Hpy8I GTNNAC 8 cut(s) 34, 92, 128, 1057, 1078, 1136, 1172, 1468
Hpy99I CGWCG 6 cut(s) 74, 817, 1118, 1330, 1355, 1706
HpyAV CCTTC 6 cut(s) 145, 560, 731, 772, 1189, 1544
HpyCH4III ACNGT 7 cut(s) 14, 70, 218, 323, 584, 777, 1114
HpyCH4IV ACGT 3 cut(s) 419, 510, 1353
HpyF10VI GCNNNNNNNGC 4 cut(s) 569, 920, 1331, 1553
HpyF3I CTNAG 6 cut(s) 84, 503, 520, 804, 1128, 1374
HpySE526I ACGT 3 cut(s) 419, 510, 1353
Hsp92I GRCGYC 1 cut(s) 1701
Hsp92II CATG 7 cut(s) 106, 482, 501, 617, 1150, 1485, 1541
HspAI GCGC 2 cut(s) 139, 1183
KspAI GTTAAC 2 cut(s) 1078, 1468
Kzo9I GATC 3 cut(s) 658, 1672, 1681
LmnI GCTCC 4 cut(s) 629, 911, 1613, 1643
Lsp1109I GCAGC 2 cut(s) 97, 1141
LweI GCATC 7 cut(s) 334, 550, 680, 1379, 1534, 1558, 1703
MaeI CTAG 2 cut(s) 287, 399
MaeII ACGT 3 cut(s) 419, 510, 1353
MaeIII GTNAC 3 cut(s) 474, 771, 832
MalI GATC 3 cut(s) 660, 1674, 1683
MboI GATC 3 cut(s) 658, 1672, 1681
MhlI GDGCHC 1 cut(s) 821
MluCI AATT 9 cut(s) 230, 484, 544, 589, 1217, 1405, 1448, 1528, 1573
MluI ACGCGT 1 cut(s) 824
MlyI GAGTC 2 cut(s) 92, 1136
MmeI TCCRAC 1 cut(s) 321
Mph1103I ATGCAT 1 cut(s) 499
MroXI GAANNNNTTC 1 cut(s) 186
MslI CAYNNNNRTG 2 cut(s) 39, 1542
MspA1I CMGCKG 3 cut(s) 685, 706, 1167
MspCI CTTAAG 1 cut(s) 965
MspI CCGG 1 cut(s) 940
MvnI CGCG 1 cut(s) 826
MwoI GCNNNNNNNGC 4 cut(s) 569, 920, 1331, 1553
NdeII GATC 3 cut(s) 658, 1672, 1681
NlaIII CATG 7 cut(s) 106, 482, 501, 617, 1150, 1485, 1541
NlaIV GGNNCC 1 cut(s) 225
NmeAIII GCCGAG 1 cut(s) 388
NmuCI GTSAC 2 cut(s) 474, 771
NsbI TGCGCA 1 cut(s) 1184
NsiI ATGCAT 1 cut(s) 499
NspI RCATGY 4 cut(s) 482, 501, 1485, 1541
PciI ACATGT 2 cut(s) 478, 1537
PcsI WCGNNNNNNNCGW 1 cut(s) 1677
PdmI GAANNNNTTC 1 cut(s) 186
PfeI GAWTC 5 cut(s) 17, 841, 1061, 1210, 1226
PkrI GCNGC 3 cut(s) 112, 705, 1156
PleI GAGTC 2 cut(s) 92, 1136
PpsI GAGTC 2 cut(s) 92, 1136
Ppu21I YACGTR 1 cut(s) 420
PscI ACATGT 2 cut(s) 478, 1537
PspEI GGTNACC 1 cut(s) 771
PspN4I GGNNCC 1 cut(s) 225
PspPI GGNCC 2 cut(s) 469, 1453
PstNI CAGNNNCTG 1 cut(s) 706
PvuII CAGCTG 1 cut(s) 685
RsaI GTAC 7 cut(s) 76, 275, 418, 689, 699, 1120, 1364
RsaNI GTAC 7 cut(s) 75, 274, 417, 688, 698, 1119, 1363
RseI CAYNNNNRTG 2 cut(s) 39, 1542
SalI GTCGAC 2 cut(s) 126, 1170
SatI GCNGC 3 cut(s) 111, 704, 1155
Sau3AI GATC 3 cut(s) 658, 1672, 1681
Sau96I GGNCC 2 cut(s) 469, 1453
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 2 cut(s) 92, 1136
SduI GDGCHC 1 cut(s) 821
SfaNI GCATC 7 cut(s) 334, 550, 680, 1379, 1534, 1558, 1703
SinI GGWCC 2 cut(s) 469, 1453
SmiMI CAYNNNNRTG 2 cut(s) 39, 1542
SmlI CTYRAG 1 cut(s) 965
SmoI CTYRAG 1 cut(s) 965
SnaBI TACGTA 1 cut(s) 420
Sse9I AATT 9 cut(s) 230, 484, 544, 589, 1217, 1405, 1448, 1528, 1573
SsiI CCGC 4 cut(s) 123, 704, 860, 1167
SspI AATATT 3 cut(s) 532, 1441, 1709
SspMI CTAG 2 cut(s) 287, 399
StyI CCWWGG 2 cut(s) 27, 1071
TaaI ACNGT 7 cut(s) 14, 70, 218, 323, 584, 777, 1114
TaiI ACGT 3 cut(s) 422, 513, 1356
TasI AATT 9 cut(s) 230, 484, 544, 589, 1217, 1405, 1448, 1528, 1573
TatI WGTACW 3 cut(s) 273, 697, 1362
TauI GCSGC 1 cut(s) 706
TfiI GAWTC 5 cut(s) 17, 841, 1061, 1210, 1226
TscAI CASTG 1 cut(s) 589
TseFI GTSAC 2 cut(s) 474, 771
TseI GCWGC 2 cut(s) 110, 1154
Tsp45I GTSAC 2 cut(s) 474, 771
TspDTI ATGAA 5 cut(s) 12, 704, 754, 994, 1056
TspGWI ACGGA 1 cut(s) 716
TspRI CASTG 1 cut(s) 589
Vha464I CTTAAG 1 cut(s) 965
VpaK11BI GGWCC 2 cut(s) 469, 1453
XapI RAATTY 1 cut(s) 589
XbaI TCTAGA 1 cut(s) 398
XceI RCATGY 4 cut(s) 482, 501, 1485, 1541
XmiI GTMKAC 3 cut(s) 127, 1056, 1171
XmnI GAANNNNTTC 1 cut(s) 186
XspI CTAG 2 cut(s) 287, 399
ZrmI AGTACT 1 cut(s) 275
Zsp2I ATGCAT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.