MD14G1005800.v1.1

FH protein interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000859
Physical Location & Seq
Reverse (-)
632114 .. 637814
5701 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1005800.v1.1.491

Sequence Viewer

Length: 1008 bp
ATGACGAAGGACTCCGATTCCTCCTCTCTGATTCGCCTTAACATCGGAGGAAAGAAATTTTGTACTACTATTGATACTTTGACTCATCGTGAGCCCGATTCGATGCTTGCTGCAATGTTCAGCGGTCGCCATAGTATATGTCAAGATGCCGAAAAGGGGTATGTGTTTCTTGATAGGGATGGCAAACATTTTCGGCACATCCTTAATTGGTTGAGGGATGGTGTTGTTCCCACGTTAAAAGATTCAATATATTCAGAACTACTTAGGGAGGCTGAATACTATCAACTTCTTGGGCTGATGGATGGAATAAATGCTGTTCTAAATAAGAAGAGGGAGGATGAAGAGTTAGATACAGAACTAACACGCACTGATATCATCAAGTGTATACAGTCTGACAGAGTGCGGTTTCGAGGTGTCAATCTTTCTGGCCTTGACCTTTCCAAATTGGACTTGTCATTGGTTGACTTCAGCTATGCATGTTTGAAAAATGTCTTTTTTTCACGTGCAAATCTTCAGTGTGCTAAGTTCAAGGATGCCGATGCTGAGGGTGCCATCTTTCACAATGCAACTTTGCGGGAATGTGAATTTACTGGGGCAAATCTCCGTGGAGCTTTGTTGCCTGGTGCTGATCTTAAGAGTGCAAATCTACAAGGCAAACTTTTCTGCATTCTGATTTCTAATAATGTTTTACTATTTTATTTGTTAGTTCCTTCATCATGTTGCCTTATATTCCTTGGCATATGTTTCTGTTCATATGCATGCTTGATAGATTCTAGCTTCTGCCAGGCAGACCTGCGTTCTGCACATCTACAGAATGCTGATCTTACAAATGCCAATTTAGAGGGAGCTATTCTGGAAGGAGCAAATTTGAAGGGTGCCAAGTTGAGTAATACCAATTTGAAGGATGCAAACCTTCAACGAGCTTATTTACGTCAAGTGAATCTTCGAGATACGCATTTGGAAGGTGCAAAGCTTGATGGTGCAAATTTGCTTGGAGCAACCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB_2 Pentapeptide

Protein Analysis

336

Amino Acids

37.16

Weight (kDa)

6.39

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB_2 PF02214 11 - 99 9.6e-19 BTB/POZ domain
Pentapeptide_4 PF13599 133 - 204 1.8e-09 Pentapeptide repeat region
Pentapeptide PF00805 150 - 188 3.3e-08 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 184 - 218 7e-08 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 251 - 282 2.7e-07 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 263 - 302 1.4e-12 Pentapeptide repeats (8 copies)
Pentapeptide_4 PF13599 264 - 330 2.2e-09 Pentapeptide repeat region
Pentapeptide PF00805 273 - 312 8.2e-13 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 303 - 333 7.4e-08 Pentapeptide repeats (8 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 801
AccB1I GGYRCC 2 cut(s) 548, 875
AccI GTMKAC 1 cut(s) 385
AciI CCGC 3 cut(s) 123, 403, 574
AcsI RAATTY 4 cut(s) 56, 584, 865, 985
AcuI CTGAAG 2 cut(s) 451, 497
AcvI CACGTG 1 cut(s) 503
AfaI GTAC 1 cut(s) 64
AfiI CCNNNNNNNGG 1 cut(s) 156
AflII CTTAAG 1 cut(s) 632
AgsI TTSAA 6 cut(s) 246, 484, 529, 871, 901, 917
AjnI CCWGG 2 cut(s) 619, 783
AluBI AGCT 6 cut(s) 471, 611, 777, 848, 923, 973
AluI AGCT 6 cut(s) 471, 611, 777, 848, 923, 973
AoxI GGCC 1 cut(s) 427
ApeKI GCWGC 1 cut(s) 110
ApoI RAATTY 4 cut(s) 56, 584, 865, 985
BanI GGYRCC 2 cut(s) 548, 875
BanII GRGCYC 1 cut(s) 96
BbrPI CACGTG 1 cut(s) 503
BbvCI CCTCAGC 1 cut(s) 543
BbvI GCAGC 1 cut(s) 97
BccI CCATC 6 cut(s) 173, 212, 292, 296, 560, 971
BciT130I CCWGG 2 cut(s) 621, 785
BfaI CTAG 1 cut(s) 774
BfmI CTRYAG 1 cut(s) 809
BfrI CTTAAG 1 cut(s) 632
BfuAI ACCTGC 1 cut(s) 801
BisI GCNGC 1 cut(s) 111
BlsI GCNGC 1 cut(s) 112
Bme1390I CCNGG 2 cut(s) 621, 785
BmiI GGNNCC 2 cut(s) 550, 877
BmrFI CCNGG 2 cut(s) 621, 785
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 5 cut(s) 93, 136, 523, 529, 895
BmuI ACTGGG 1 cut(s) 600
Bpu10I CCTNAGC 1 cut(s) 543
BsaAI YACGTR 1 cut(s) 503
BsaJI CCNNGG 2 cut(s) 604, 733
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 1 cut(s) 595
Bse3DI GCAATG 1 cut(s) 120
BseBI CCWGG 2 cut(s) 621, 785
BseDI CCNNGG 2 cut(s) 604, 733
BseGI GGATG 7 cut(s) 184, 198, 223, 307, 343, 538, 910
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 1 cut(s) 595
BseRI GAGGAG 1 cut(s) 13
BseXI GCAGC 1 cut(s) 97
BsgI GTGCAG 1 cut(s) 786
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 1 cut(s) 429
BshNI GGYRCC 2 cut(s) 548, 875
BsiEI CGRYCG 1 cut(s) 127
BslI CCNNNNNNNGG 1 cut(s) 156
BsmI GAATGC 2 cut(s) 666, 820
BsnI GGCC 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 2 cut(s) 628, 820
BspACI CCGC 3 cut(s) 123, 403, 574
BspANI GGCC 1 cut(s) 429
BspCNI CTCAG 1 cut(s) 535
BspLI GGNNCC 2 cut(s) 550, 877
BspMI ACCTGC 1 cut(s) 801
BspT107I GGYRCC 2 cut(s) 548, 875
BspTI CTTAAG 1 cut(s) 632
BsrDI GCAATG 1 cut(s) 120
BsrI ACTGG 1 cut(s) 595
BssECI CCNNGG 2 cut(s) 604, 733
BssMI GATC 2 cut(s) 628, 820
BssNAI GTATAC 1 cut(s) 386
BssT1I CCWWGG 1 cut(s) 733
Bst1107I GTATAC 1 cut(s) 386
Bst2UI CCWGG 2 cut(s) 621, 785
Bst4CI ACNGT 1 cut(s) 390
Bst6I CTCTTC 2 cut(s) 323, 336
BstAFI CTTAAG 1 cut(s) 632
BstBAI YACGTR 1 cut(s) 503
BstC8I GCNNGC 2 cut(s) 108, 760
BstDEI CTNAG 3 cut(s) 263, 522, 543
BstDSI CCRYGG 1 cut(s) 604
BstF5I GGATG 7 cut(s) 184, 198, 223, 307, 343, 538, 910
BstKTI GATC 2 cut(s) 631, 823
BstMBI GATC 2 cut(s) 628, 820
BstMCI CGRYCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 1 cut(s) 548
BstNI CCWGG 2 cut(s) 621, 785
BstNSI RCATGY 2 cut(s) 480, 762
BstSCI CCNGG 2 cut(s) 619, 783
BstSFI CTRYAG 1 cut(s) 809
BstV1I GCAGC 1 cut(s) 97
BstZ17I GTATAC 1 cut(s) 386
BsuRI GGCC 1 cut(s) 429
BtgI CCRYGG 1 cut(s) 604
BtsCI GGATG 7 cut(s) 184, 198, 223, 307, 343, 538, 910
BtsIMutI CAGTG 2 cut(s) 366, 521
BveI ACCTGC 1 cut(s) 801
Cac8I GCNNGC 2 cut(s) 108, 760
Csp6I GTAC 1 cut(s) 63
CviAII CATG 3 cut(s) 477, 717, 759
CviQI GTAC 1 cut(s) 63
DdeI CTNAG 3 cut(s) 263, 522, 543
DpnI GATC 2 cut(s) 630, 822
DpnII GATC 2 cut(s) 628, 820
Eam1104I CTCTTC 2 cut(s) 323, 336
EarI CTCTTC 2 cut(s) 323, 336
Eco130I CCWWGG 1 cut(s) 733
Eco24I GRGCYC 1 cut(s) 96
Eco32I GATATC 1 cut(s) 373
Eco57I CTGAAG 2 cut(s) 451, 497
Eco72I CACGTG 1 cut(s) 503
EcoRII CCWGG 2 cut(s) 619, 783
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 733
EcoT22I ATGCAT 2 cut(s) 478, 760
EcoT38I GRGCYC 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 733
FaeI CATG 3 cut(s) 480, 720, 762
FalI AAGNNNNNCTT 4 cut(s) 642, 674, 927, 959
FatI CATG 3 cut(s) 476, 716, 758
FauI CCCGC 1 cut(s) 567
FauNDI CATATG 2 cut(s) 740, 754
FblI GTMKAC 1 cut(s) 385
Fnu4HI GCNGC 1 cut(s) 111
FokI GGATG 7 cut(s) 185, 191, 230, 314, 350, 545, 917
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 1 cut(s) 774
GluI GCNGC 1 cut(s) 111
HaeIII GGCC 1 cut(s) 429
Hin1II CATG 3 cut(s) 480, 720, 762
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HindIII AAGCTT 1 cut(s) 971
HinfI GANTC 8 cut(s) 11, 17, 31, 82, 98, 242, 770, 940
Hpy166II GTNNAC 2 cut(s) 386, 463
Hpy188I TCNGA 6 cut(s) 16, 30, 47, 256, 394, 672
Hpy188III TCNNGA 5 cut(s) 89, 143, 170, 854, 947
Hpy8I GTNNAC 2 cut(s) 386, 463
HpyAV CCTTC 6 cut(s) 720, 851, 865, 895, 923, 956
HpyCH4III ACNGT 1 cut(s) 390
HpyCH4IV ACGT 3 cut(s) 233, 502, 931
HpyF10VI GCNNNNNNNGC 1 cut(s) 548
HpyF3I CTNAG 3 cut(s) 263, 522, 543
HpySE526I ACGT 3 cut(s) 233, 502, 931
Hsp92II CATG 3 cut(s) 480, 720, 762
Kzo9I GATC 2 cut(s) 628, 820
LmnI GCTCC 4 cut(s) 608, 845, 860, 995
LpnPI CCDG 8 cut(s) 411, 576, 606, 633, 770, 797, 806, 839
Lsp1109I GCAGC 1 cut(s) 97
LweI GCATC 5 cut(s) 93, 136, 523, 529, 895
MaeI CTAG 1 cut(s) 774
MaeII ACGT 3 cut(s) 233, 502, 931
MalI GATC 2 cut(s) 630, 822
MboI GATC 2 cut(s) 628, 820
MboII GAAGA 4 cut(s) 340, 353, 503, 935
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 8 cut(s) 56, 205, 443, 584, 835, 865, 895, 985
MlyI GAGTC 2 cut(s) 5, 76
Mph1103I ATGCAT 2 cut(s) 478, 760
MseI TTAA 4 cut(s) 39, 204, 236, 633
MslI CAYNNNNRTG 1 cut(s) 757
MspA1I CMGCKG 1 cut(s) 123
MspCI CTTAAG 1 cut(s) 632
MspR9I CCNGG 2 cut(s) 621, 785
Mva1269I GAATGC 2 cut(s) 666, 820
MvaI CCWGG 2 cut(s) 621, 785
MwoI GCNNNNNNNGC 1 cut(s) 548
NdeI CATATG 2 cut(s) 740, 754
NdeII GATC 2 cut(s) 628, 820
NlaIII CATG 3 cut(s) 480, 720, 762
NlaIV GGNNCC 2 cut(s) 550, 877
NsiI ATGCAT 2 cut(s) 478, 760
NspI RCATGY 2 cut(s) 480, 762
PaeI GCATGC 1 cut(s) 762
PctI GAATGC 2 cut(s) 666, 820
PfeI GAWTC 6 cut(s) 17, 31, 98, 242, 770, 940
PkrI GCNGC 1 cut(s) 112
PleI GAGTC 2 cut(s) 5, 76
PmaCI CACGTG 1 cut(s) 503
PmlI CACGTG 1 cut(s) 503
PpsI GAGTC 2 cut(s) 5, 76
Ppu21I YACGTR 1 cut(s) 503
Psp6I CCWGG 2 cut(s) 619, 783
PspCI CACGTG 1 cut(s) 503
PspGI CCWGG 2 cut(s) 619, 783
PspN4I GGNNCC 2 cut(s) 550, 877
RsaI GTAC 1 cut(s) 64
RsaNI GTAC 1 cut(s) 63
RseI CAYNNNNRTG 1 cut(s) 757
SaqAI TTAA 4 cut(s) 39, 204, 236, 633
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 2 cut(s) 628, 820
SchI GAGTC 2 cut(s) 5, 76
ScrFI CCNGG 2 cut(s) 621, 785
SduI GDGCHC 1 cut(s) 96
SfaNI GCATC 5 cut(s) 93, 136, 523, 529, 895
SfcI CTRYAG 1 cut(s) 809
SmiMI CAYNNNNRTG 1 cut(s) 757
SmlI CTYRAG 1 cut(s) 632
SmoI CTYRAG 1 cut(s) 632
SphI GCATGC 1 cut(s) 762
Sse9I AATT 8 cut(s) 56, 205, 443, 584, 835, 865, 895, 985
SsiI CCGC 3 cut(s) 123, 403, 574
SspMI CTAG 1 cut(s) 774
StyD4I CCNGG 2 cut(s) 619, 783
StyI CCWWGG 1 cut(s) 733
TaaI ACNGT 1 cut(s) 390
TaiI ACGT 3 cut(s) 236, 505, 934
TaqI TCGA 3 cut(s) 101, 409, 946
TasI AATT 8 cut(s) 56, 205, 443, 584, 835, 865, 895, 985
TatI WGTACW 1 cut(s) 62
TfiI GAWTC 6 cut(s) 17, 31, 98, 242, 770, 940
Tru1I TTAA 4 cut(s) 39, 204, 236, 633
Tru9I TTAA 4 cut(s) 39, 204, 236, 633
TscAI CASTG 2 cut(s) 373, 521
TseI GCWGC 1 cut(s) 110
TspDTI ATGAA 3 cut(s) 354, 702, 741
TspGWI ACGGA 1 cut(s) 593
TspRI CASTG 2 cut(s) 373, 521
Vha464I CTTAAG 1 cut(s) 632
XapI RAATTY 4 cut(s) 56, 584, 865, 985
XceI RCATGY 2 cut(s) 480, 762
XmiI GTMKAC 1 cut(s) 385
XspI CTAG 1 cut(s) 774
Zsp2I ATGCAT 2 cut(s) 478, 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.