MD12G1008500.v1.1

FH protein interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000859
Physical Location & Seq
Reverse (-)
920563 .. 926259
5697 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1008500.v1.1.491

Sequence Viewer

Length: 876 bp
ATGACGAAGGTCTCCGAATCATCCTCTCTGATTCGCCTCAACATCGGAGGAAAGAAATTTTGTACTACTATTGATACTTTGACTCGTCGTGAGCCCGATTCGTTGCTTGCTGCAATGTTCAGTGGTCGCCATAGTCTATGTCAAGAAGCCGAAAAGGGATATGTTTTTCTTGATAGGGATGGCAAACATTTTCGGCACATCCTTAACTGGTTGAGGGATGGCGTTGTTCCCACATTAAAAGATTCAGAATATTCAGAACTACTTAGGGAGGCTGAATACTATCAACTTCTAGGTCTGATGGATGGAATACATGCTGTTCTAAATAAGAAGAGGGAGGATGAAGAGGTAGATACAGAACTAACACGTACTGATATCATCAAGTGCATACAGTCTGAGAAAGTGCGTTTTCGAGGTGTCAATCTTTCTGGCCTTGACCTTTCCAAATTGGACTTGTCATTTGTTGACTTCAGCTATGCATGTTTGAAAAATGTCTTTTTCTCACGTGCAAATCTTCAATGTGCTAAGTTCAAGGATGTTGATGCTGAGGGTGCCATCTTTCACAATGCAACTTTGCGGGAATGTGAATTTACTGGGGCAAATCTCCGTGGAGCTTTGTTGGCTGATGCATGCTTGATAGATTCTAGCTTCTGCCAGGCAGACCTGCGTTCTGCACACCTACAGAATGCTGATCTTACAAATGCCAATCTAGAGGGAGCTATTCTGGAAGGAGCAAATTTGAAGGGTGCCAAGTTGAGGAATACCAATTTGAAGGATGCAAACCTTCAACGAGCTTATTTACGTCAAGTGAATCTTCGAGATACGAATTTGGAAGGTGCAAAGCTTGATGGTGCAAATTTGCTTGGAGCAATGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB_2 Pentapeptide

Protein Analysis

292

Amino Acids

32.51

Weight (kDa)

6.33

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB_2 PF02214 11 - 99 2e-19 BTB/POZ domain
Pentapeptide_4 PF13599 132 - 204 7e-09 Pentapeptide repeat region
Pentapeptide PF00805 150 - 188 4.4e-08 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 169 - 206 2.6e-08 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 184 - 222 1.3e-07 Pentapeptide repeats (8 copies)
Pentapeptide_4 PF13599 184 - 261 9.9e-12 Pentapeptide repeat region
Pentapeptide PF00805 200 - 238 5e-09 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 219 - 258 8e-13 Pentapeptide repeats (8 copies)
Pentapeptide_4 PF13599 220 - 285 2e-09 Pentapeptide repeat region
Pentapeptide PF00805 259 - 289 1.6e-08 Pentapeptide repeats (8 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 669
AccB1I GGYRCC 2 cut(s) 548, 743
AciI CCGC 1 cut(s) 574
AcsI RAATTY 5 cut(s) 56, 584, 733, 823, 853
AcuI CTGAAG 1 cut(s) 451
AcvI CACGTG 1 cut(s) 503
AfaI GTAC 2 cut(s) 64, 367
AfiI CCNNNNNNNGG 1 cut(s) 753
AflIII ACRYGT 1 cut(s) 362
AgsI TTSAA 6 cut(s) 484, 515, 529, 739, 769, 785
AjnI CCWGG 1 cut(s) 651
AluBI AGCT 6 cut(s) 471, 611, 645, 716, 791, 841
AluI AGCT 6 cut(s) 471, 611, 645, 716, 791, 841
Alw26I GTCTC 1 cut(s) 16
AoxI GGCC 1 cut(s) 427
ApeKI GCWGC 1 cut(s) 110
ApoI RAATTY 5 cut(s) 56, 584, 733, 823, 853
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
BanI GGYRCC 2 cut(s) 548, 743
BanII GRGCYC 1 cut(s) 96
BbrPI CACGTG 1 cut(s) 503
BbvCI CCTCAGC 1 cut(s) 543
BbvI GCAGC 1 cut(s) 97
BccI CCATC 6 cut(s) 173, 212, 292, 296, 560, 839
BciT130I CCWGG 1 cut(s) 653
BcoDI GTCTC 1 cut(s) 16
BfaI CTAG 3 cut(s) 290, 642, 707
BfmI CTRYAG 1 cut(s) 677
BfuAI ACCTGC 1 cut(s) 669
BisI GCNGC 1 cut(s) 111
BlsI GCNGC 1 cut(s) 112
Bme1390I CCNGG 1 cut(s) 653
BmiI GGNNCC 2 cut(s) 550, 745
BmrFI CCNGG 1 cut(s) 653
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 3 cut(s) 529, 613, 763
BmuI ACTGGG 1 cut(s) 600
BoxI GACNNNNGTC 1 cut(s) 8
Bpu10I CCTNAGC 1 cut(s) 543
BsaAI YACGTR 2 cut(s) 365, 503
BsaI GGTCTC 1 cut(s) 16
BsaJI CCNNGG 1 cut(s) 604
Bsc4I CCNNNNNNNGG 1 cut(s) 753
Bse1I ACTGG 2 cut(s) 212, 595
Bse3DI GCAATG 2 cut(s) 120, 873
BseBI CCWGG 1 cut(s) 653
BseDI CCNNGG 1 cut(s) 604
BseGI GGATG 8 cut(s) 20, 184, 198, 223, 307, 343, 538, 778
BseLI CCNNNNNNNGG 1 cut(s) 753
BseMI GCAATG 2 cut(s) 120, 873
BseMII CTCAG 2 cut(s) 384, 534
BseNI ACTGG 2 cut(s) 212, 595
BseXI GCAGC 1 cut(s) 97
BsgI GTGCAG 1 cut(s) 654
BshFI GGCC 1 cut(s) 429
BshNI GGYRCC 2 cut(s) 548, 743
BslI CCNNNNNNNGG 1 cut(s) 753
BsmAI GTCTC 1 cut(s) 16
BsmI GAATGC 1 cut(s) 688
BsnI GGCC 1 cut(s) 429
Bso31I GGTCTC 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 1 cut(s) 688
BspACI CCGC 1 cut(s) 574
BspANI GGCC 1 cut(s) 429
BspCNI CTCAG 2 cut(s) 385, 535
BspLI GGNNCC 2 cut(s) 550, 745
BspMI ACCTGC 1 cut(s) 669
BspT107I GGYRCC 2 cut(s) 548, 743
BspTNI GGTCTC 1 cut(s) 16
BsrDI GCAATG 2 cut(s) 120, 873
BsrI ACTGG 2 cut(s) 212, 595
BssECI CCNNGG 1 cut(s) 604
BssMI GATC 1 cut(s) 688
Bst2UI CCWGG 1 cut(s) 653
Bst4CI ACNGT 1 cut(s) 390
Bst6I CTCTTC 2 cut(s) 323, 336
BstBAI YACGTR 2 cut(s) 365, 503
BstC8I GCNNGC 2 cut(s) 108, 628
BstDEI CTNAG 4 cut(s) 263, 393, 522, 543
BstDSI CCRYGG 1 cut(s) 604
BstF5I GGATG 8 cut(s) 20, 184, 198, 223, 307, 343, 538, 778
BstKTI GATC 1 cut(s) 691
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 1 cut(s) 688
BstMWI GCNNNNNNNGC 2 cut(s) 548, 617
BstNI CCWGG 1 cut(s) 653
BstNSI RCATGY 3 cut(s) 314, 480, 630
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 651
BstSFI CTRYAG 1 cut(s) 677
BstV1I GCAGC 1 cut(s) 97
BsuRI GGCC 1 cut(s) 429
BtgI CCRYGG 1 cut(s) 604
BtsCI GGATG 8 cut(s) 20, 184, 198, 223, 307, 343, 538, 778
BtsIMutI CAGTG 1 cut(s) 127
BveI ACCTGC 1 cut(s) 669
Cac8I GCNNGC 2 cut(s) 108, 628
Csp6I GTAC 2 cut(s) 63, 366
CviAII CATG 3 cut(s) 311, 477, 627
CviQI GTAC 2 cut(s) 63, 366
DdeI CTNAG 4 cut(s) 263, 393, 522, 543
DpnI GATC 1 cut(s) 690
DpnII GATC 1 cut(s) 688
Eam1104I CTCTTC 2 cut(s) 323, 336
EarI CTCTTC 2 cut(s) 323, 336
Eco24I GRGCYC 1 cut(s) 96
Eco31I GGTCTC 1 cut(s) 16
Eco32I GATATC 1 cut(s) 373
Eco57I CTGAAG 1 cut(s) 451
Eco72I CACGTG 1 cut(s) 503
EcoRII CCWGG 1 cut(s) 651
EcoRV GATATC 1 cut(s) 373
EcoT22I ATGCAT 2 cut(s) 478, 628
EcoT38I GRGCYC 1 cut(s) 96
FaeI CATG 3 cut(s) 314, 480, 630
FaiI YATR 8 cut(s) 132, 139, 162, 312, 386, 474, 478, 628
FalI AAGNNNNNCTT 2 cut(s) 795, 827
FatI CATG 3 cut(s) 310, 476, 626
FauI CCCGC 1 cut(s) 567
Fnu4HI GCNGC 1 cut(s) 111
FokI GGATG 8 cut(s) 7, 185, 191, 230, 314, 350, 545, 785
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 3 cut(s) 290, 642, 707
GluI GCNGC 1 cut(s) 111
HaeIII GGCC 1 cut(s) 429
Hin1II CATG 3 cut(s) 314, 480, 630
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HindIII AAGCTT 1 cut(s) 839
HinfI GANTC 7 cut(s) 17, 31, 82, 98, 242, 638, 808
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 7 cut(s) 16, 30, 47, 247, 256, 297, 394
Hpy188III TCNNGA 6 cut(s) 89, 143, 170, 707, 722, 815
Hpy8I GTNNAC 1 cut(s) 463
Hpy99I CGWCG 1 cut(s) 90
HpyAV CCTTC 5 cut(s) 719, 733, 763, 791, 824
HpyCH4III ACNGT 1 cut(s) 390
HpyCH4IV ACGT 3 cut(s) 364, 502, 799
HpyF10VI GCNNNNNNNGC 2 cut(s) 548, 617
HpyF3I CTNAG 4 cut(s) 263, 393, 522, 543
HpySE526I ACGT 3 cut(s) 364, 502, 799
Hsp92II CATG 3 cut(s) 314, 480, 630
Kzo9I GATC 1 cut(s) 688
LmnI GCTCC 4 cut(s) 608, 713, 728, 863
LpnPI CCDG 7 cut(s) 193, 411, 576, 638, 665, 674, 707
Lsp1109I GCAGC 1 cut(s) 97
LweI GCATC 3 cut(s) 529, 613, 763
MaeI CTAG 3 cut(s) 290, 642, 707
MaeII ACGT 3 cut(s) 364, 502, 799
MalI GATC 1 cut(s) 690
MboI GATC 1 cut(s) 688
MboII GAAGA 4 cut(s) 340, 353, 503, 803
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 7 cut(s) 56, 443, 584, 733, 763, 823, 853
MlyI GAGTC 1 cut(s) 76
Mph1103I ATGCAT 2 cut(s) 478, 628
MseI TTAA 2 cut(s) 204, 236
MspR9I CCNGG 1 cut(s) 653
Mva1269I GAATGC 1 cut(s) 688
MvaI CCWGG 1 cut(s) 653
MwoI GCNNNNNNNGC 2 cut(s) 548, 617
NdeII GATC 1 cut(s) 688
NlaIII CATG 3 cut(s) 314, 480, 630
NlaIV GGNNCC 2 cut(s) 550, 745
NsiI ATGCAT 2 cut(s) 478, 628
NspI RCATGY 3 cut(s) 314, 480, 630
PaeI GCATGC 1 cut(s) 630
PctI GAATGC 1 cut(s) 688
PfeI GAWTC 6 cut(s) 17, 31, 98, 242, 638, 808
PkrI GCNGC 1 cut(s) 112
PleI GAGTC 1 cut(s) 76
PmaCI CACGTG 1 cut(s) 503
PmlI CACGTG 1 cut(s) 503
PpsI GAGTC 1 cut(s) 76
Ppu21I YACGTR 2 cut(s) 365, 503
PshAI GACNNNNGTC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 651
PspCI CACGTG 1 cut(s) 503
PspGI CCWGG 1 cut(s) 651
PspN4I GGNNCC 2 cut(s) 550, 745
RsaI GTAC 2 cut(s) 64, 367
RsaNI GTAC 2 cut(s) 63, 366
SaqAI TTAA 2 cut(s) 204, 236
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 1 cut(s) 688
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 1 cut(s) 653
SduI GDGCHC 1 cut(s) 96
SfaNI GCATC 3 cut(s) 529, 613, 763
SfcI CTRYAG 1 cut(s) 677
SphI GCATGC 1 cut(s) 630
Sse9I AATT 7 cut(s) 56, 443, 584, 733, 763, 823, 853
SsiI CCGC 1 cut(s) 574
SspI AATATT 1 cut(s) 251
SspMI CTAG 3 cut(s) 290, 642, 707
StyD4I CCNGG 1 cut(s) 651
TaaI ACNGT 1 cut(s) 390
TaiI ACGT 3 cut(s) 367, 505, 802
TaqI TCGA 2 cut(s) 409, 814
TasI AATT 7 cut(s) 56, 443, 584, 733, 763, 823, 853
TatI WGTACW 1 cut(s) 62
TfiI GAWTC 6 cut(s) 17, 31, 98, 242, 638, 808
Tru1I TTAA 2 cut(s) 204, 236
Tru9I TTAA 2 cut(s) 204, 236
TscAI CASTG 1 cut(s) 127
TseI GCWGC 1 cut(s) 110
TspDTI ATGAA 1 cut(s) 354
TspGWI ACGGA 1 cut(s) 593
TspRI CASTG 1 cut(s) 127
XapI RAATTY 5 cut(s) 56, 584, 733, 823, 853
XbaI TCTAGA 1 cut(s) 706
XceI RCATGY 3 cut(s) 314, 480, 630
XspI CTAG 3 cut(s) 290, 642, 707
Zsp2I ATGCAT 2 cut(s) 478, 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.