MD03G1044000.v1.1

FH protein interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000859
Physical Location & Seq
Reverse (-)
3480700 .. 3482444
1745 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1044000.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGCAGAGGACTGCGATTCTTCATCCTTGGTTCACCTCAATGTGGGAGGGAAGAAGTTTTCCACCACCGTCGATACTCTAACTCAGCGTCAACCCGACTCCATGCTTGCTGCAATGTTTAGCGGTCGACACACTTTGCGCCAAGATAAGAAGGGATATGTTTTTATCGATAGAGATGGCGAACATTTTCGGCACATTCTTAACTGGTTAAGAGACGGTGAGATTCCTAAATTAGAAGCTTCCCAATATGCAGAACTTGTTAAGGAGGCAAGGTACTATCAACTACTAGGGTTAGTTGATGGAATACCTGATGTCCTGAACAGTAAGGAAGATAAGAAGTTGGATGCAGATTTTACCCGTGCCGAGGTTATCAACTACATATATTCAATGAAGTGGTCTGGACGCAAGAATGTGTGTGTGTCTAAGCCTTGTTTTCGAGGGGTTAATCTTTCTGGCCTTAATCTTTCAAAACTGGACCTGTCATATGTTGACTTCAGCTATGCATGTCTGAGACATGTGTGTTTCTCAGGTGCACATATTACATATTCTAATTTCGACTACGCTGATGCTGAAGGGGCTACCTTTGACAATGCACATTTGAGTCAGTCTACTTTTTCTGAAGCAAATCTTCGAGGAGCTTCTTTTGTGGGTGCTAAACACTACTATCTCGCTGGGGCATCTTGCGTGGAAGATTGTAGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB_2 Pentapeptide

Protein Analysis

235

Amino Acids

26.32

Weight (kDa)

6.23

Isoelectric Point (pI)

31.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB_2 PF02214 11 - 98 1.2e-20 BTB/POZ domain
Pentapeptide_4 PF13599 139 - 196 7e-06 Pentapeptide repeat region
Pentapeptide PF00805 145 - 177 5e-07 Pentapeptide repeats (8 copies)
Pentapeptide PF00805 183 - 218 1e-07 Pentapeptide repeats (8 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 127, 608
AciI CCGC 1 cut(s) 123
AcuI CTGAAG 3 cut(s) 478, 591, 639
AfaI GTAC 2 cut(s) 275, 701
AfiI CCNNNNNNNGG 2 cut(s) 43, 364
AflIII ACRYGT 1 cut(s) 514
AgsI TTSAA 2 cut(s) 387, 468
AluBI AGCT 3 cut(s) 239, 498, 638
AluI AGCT 3 cut(s) 239, 498, 638
Alw21I GWGCWC 1 cut(s) 535
Alw26I GTCTC 2 cut(s) 207, 505
Alw44I GTGCAC 1 cut(s) 531
AoxI GGCC 1 cut(s) 454
ApaLI GTGCAC 1 cut(s) 531
ApeKI GCWGC 1 cut(s) 110
Asp700I GAANNNNTTC 1 cut(s) 186
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 475
AsuHPI GGTGA 2 cut(s) 26, 230
AvaII GGWCC 1 cut(s) 475
BaeGI GKGCMC 1 cut(s) 535
Bbv12I GWGCWC 1 cut(s) 535
BbvI GCAGC 1 cut(s) 97
BccI CCATC 2 cut(s) 170, 293
BcoDI GTCTC 2 cut(s) 207, 505
BfaI CTAG 1 cut(s) 287
BisI GCNGC 1 cut(s) 111
BlsI GCNGC 1 cut(s) 112
Bme18I GGWCC 1 cut(s) 475
BmgT120I GGNCC 1 cut(s) 475
BmsI GCATC 3 cut(s) 334, 556, 686
Bsa29I ATCGAT 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 27, 363
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 364
Bse1I ACTGG 2 cut(s) 209, 477
Bse3DI GCAATG 1 cut(s) 120
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 27, 363
BseGI GGATG 2 cut(s) 23, 349
BseLI CCNNNNNNNGG 2 cut(s) 43, 364
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 3 cut(s) 98, 500, 540
BseNI ACTGG 2 cut(s) 209, 477
BseRI GAGGAG 1 cut(s) 648
BseSI GKGCMC 1 cut(s) 535
BseXI GCAGC 1 cut(s) 97
BseYI CCCAGC 1 cut(s) 671
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 1 cut(s) 456
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 535
BslI CCNNNNNNNGG 2 cut(s) 43, 364
BsmAI GTCTC 2 cut(s) 207, 505
BsmBI CGTCTC 1 cut(s) 207
BsnI GGCC 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 535
BspACI CCGC 1 cut(s) 123
BspANI GGCC 1 cut(s) 456
BspCNI CTCAG 3 cut(s) 97, 501, 539
BspDI ATCGAT 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 120
BsrI ACTGG 2 cut(s) 209, 477
BssECI CCNNGG 2 cut(s) 27, 363
BssT1I CCWWGG 1 cut(s) 27
Bst4CI ACNGT 3 cut(s) 70, 218, 323
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 4 cut(s) 84, 423, 509, 526
BstF5I GGATG 2 cut(s) 23, 349
BstHHI GCGC 1 cut(s) 141
BstMAI GTCTC 2 cut(s) 207, 505
BstMCI CGRYCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 1 cut(s) 575
BstNSI RCATGY 2 cut(s) 507, 518
BstSLI GKGCMC 1 cut(s) 535
BstV1I GCAGC 1 cut(s) 97
Bsu15I ATCGAT 1 cut(s) 168
BsuRI GGCC 1 cut(s) 456
BsuTUI ATCGAT 1 cut(s) 168
BtsCI GGATG 2 cut(s) 23, 349
Cac8I GCNNGC 1 cut(s) 108
CfoI GCGC 1 cut(s) 141
Cfr13I GGNCC 1 cut(s) 475
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 2 cut(s) 77, 411
Csp6I GTAC 2 cut(s) 274, 700
CviAII CATG 3 cut(s) 103, 504, 515
CviJI RGCY 6 cut(s) 239, 427, 456, 498, 578, 638
CviKI_1 RGCY 6 cut(s) 239, 427, 456, 498, 578, 638
CviQI GTAC 2 cut(s) 274, 700
DdeI CTNAG 4 cut(s) 84, 423, 509, 526
Eco130I CCWWGG 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 475
Eco57I CTGAAG 3 cut(s) 478, 591, 639
EcoT14I CCWWGG 1 cut(s) 27
EcoT22I ATGCAT 1 cut(s) 505
ErhI CCWWGG 1 cut(s) 27
Esp3I CGTCTC 1 cut(s) 207
FaeI CATG 3 cut(s) 106, 507, 518
FalI AAGNNNNNCTT 2 cut(s) 612, 644
FatI CATG 3 cut(s) 102, 503, 514
FauNDI CATATG 1 cut(s) 484
FblI GTMKAC 2 cut(s) 127, 608
Fnu4HI GCNGC 1 cut(s) 111
FokI GGATG 2 cut(s) 10, 356
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 1 cut(s) 287
GlaI GCGC 1 cut(s) 140
GluI GCNGC 1 cut(s) 111
GsaI CCCAGC 1 cut(s) 675
HaeIII GGCC 1 cut(s) 456
HgaI GACGC 2 cut(s) 77, 411
HhaI GCGC 1 cut(s) 141
Hin1II CATG 3 cut(s) 106, 507, 518
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HincII GTYRAC 3 cut(s) 92, 128, 490
HindII GTYRAC 3 cut(s) 92, 128, 490
HindIII AAGCTT 1 cut(s) 237
HinfI GANTC 4 cut(s) 17, 98, 223, 601
HphI GGTGA 2 cut(s) 26, 230
Hpy166II GTNNAC 6 cut(s) 34, 92, 128, 490, 533, 609
Hpy188I TCNGA 2 cut(s) 510, 619
Hpy188III TCNNGA 2 cut(s) 316, 399
Hpy8I GTNNAC 6 cut(s) 34, 92, 128, 490, 533, 609
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 2 cut(s) 145, 566
HpyCH4III ACNGT 3 cut(s) 70, 218, 323
HpyCH4V TGCA 6 cut(s) 113, 251, 347, 503, 533, 593
HpyF10VI GCNNNNNNNGC 1 cut(s) 575
HpyF3I CTNAG 4 cut(s) 84, 423, 509, 526
Hsp92II CATG 3 cut(s) 106, 507, 518
HspAI GCGC 1 cut(s) 139
LmnI GCTCC 1 cut(s) 635
LpnPI CCDG 9 cut(s) 190, 321, 329, 384, 438, 458, 491, 513, 657
Lsp1109I GCAGC 1 cut(s) 97
LweI GCATC 3 cut(s) 334, 556, 686
MaeI CTAG 1 cut(s) 287
MboII GAAGA 5 cut(s) 12, 64, 341, 620, 701
MhlI GDGCHC 1 cut(s) 535
MluCI AATT 2 cut(s) 230, 550
MlyI GAGTC 2 cut(s) 92, 610
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 6 cut(s) 41, 47, 259, 358, 431, 626
Mph1103I ATGCAT 1 cut(s) 505
MroXI GAANNNNTTC 1 cut(s) 186
MseI TTAA 5 cut(s) 201, 209, 261, 444, 459
MslI CAYNNNNRTG 1 cut(s) 39
MwoI GCNNNNNNNGC 1 cut(s) 575
NdeI CATATG 1 cut(s) 484
NlaIII CATG 3 cut(s) 106, 507, 518
NmeAIII GCCGAG 1 cut(s) 388
NsiI ATGCAT 1 cut(s) 505
NspI RCATGY 2 cut(s) 507, 518
PciI ACATGT 1 cut(s) 514
PdmI GAANNNNTTC 1 cut(s) 186
PfeI GAWTC 2 cut(s) 17, 223
PkrI GCNGC 1 cut(s) 112
PleI GAGTC 2 cut(s) 92, 609
PpsI GAGTC 2 cut(s) 92, 609
PscI ACATGT 1 cut(s) 514
PspFI CCCAGC 1 cut(s) 671
PspPI GGNCC 1 cut(s) 475
RsaI GTAC 2 cut(s) 275, 701
RsaNI GTAC 2 cut(s) 274, 700
RseI CAYNNNNRTG 1 cut(s) 39
SalI GTCGAC 1 cut(s) 126
SaqAI TTAA 5 cut(s) 201, 209, 261, 444, 459
SatI GCNGC 1 cut(s) 111
Sau96I GGNCC 1 cut(s) 475
SchI GAGTC 2 cut(s) 92, 610
SduI GDGCHC 1 cut(s) 535
SfaNI GCATC 3 cut(s) 334, 556, 686
SinI GGWCC 1 cut(s) 475
SmiMI CAYNNNNRTG 1 cut(s) 39
Sse9I AATT 2 cut(s) 230, 550
SsiI CCGC 1 cut(s) 123
SspMI CTAG 1 cut(s) 287
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 3 cut(s) 70, 218, 323
TaqI TCGA 6 cut(s) 72, 127, 168, 436, 555, 631
TasI AATT 2 cut(s) 230, 550
TfiI GAWTC 2 cut(s) 17, 223
Tru1I TTAA 5 cut(s) 201, 209, 261, 444, 459
Tru9I TTAA 5 cut(s) 201, 209, 261, 444, 459
TseI GCWGC 1 cut(s) 110
TspDTI ATGAA 2 cut(s) 12, 404
VneI GTGCAC 1 cut(s) 531
VpaK11BI GGWCC 1 cut(s) 475
XceI RCATGY 2 cut(s) 507, 518
XmiI GTMKAC 2 cut(s) 127, 608
XmnI GAANNNNTTC 1 cut(s) 186
XspI CTAG 1 cut(s) 287
Zsp2I ATGCAT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.