Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0000859

Physical Location & Seq

Genomic Coordinates Forward (+)

3788904 .. 3789659

Gene Structure 756 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1047000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 756 bp

ATGGCGAAGGACCTTAACATCTTTTCTACAGTTTATCTCAAGATCGGAGTGAGGGAGTTTTGTACAACGGTTGATACTCTAACTCGGCGTGAGCCTAATTCAATGCTTGCTGCAATGTTTAGTGGACGTCATGTTGTCCATCAGGACCCGGAAACGGGATTTGTTTTCATCGACAGGGATGGCAAACATTTTCGGCACATCCTTAACTGGTTAAGGGATGGAGTTGTTCCCTCGCCGGAAGAATTTTCCAAATATTCGGAACTTGTAAGAGAGGCGGAGTATTATCAACTACTTGGTCTAAGAGATATAATACAGGCTTTTCTAAACATGAGGAAGGATGATGATCAAAACACTGAAAAGTTGGGTGCAGAACTAACGCGCATTGATATCATCAAATCATGTATAAAATCAAATGAGAGAATCCGGCTTCGAGGCGTTAACCTCTCCGGCCTTAACCTTTCGAACTTGGACTTGACAAACATTGACTTCAGTTATGCATGTCTGAGAAATGTGTGTTTCAAGGGTTGTGATCTTCGAGGTTCAAACTTTAATCACGCGGATGCTGAGGGTGCCATCTTTCAACATGCTAGTCTGCAAAGTTCTGAATTTATTGGGGCGAATCTCCGCAACGCTTTATTTGTTGGTGCTAACCTTTACAACGCAAATTTGATATCTGCAGAGCTCCATGGTGCTGATCTTACAAATGCAAATTTAGAGGCGGCCAGTCTGGATAAGGCATACTTGATGAGTCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BTB_2 Pentapeptide

Protein Analysis

252

Amino Acids

28.24

Weight (kDa)

5.82

Isoelectric Point (pI)

36.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB_2	PF02214	11 - 98	2e-15	BTB/POZ domain
Pentapeptide	PF00805	142 - 180	5.7e-10	Pentapeptide repeats (8 copies)
Pentapeptide_4	PF13599	144 - 203	3.1e-07	Pentapeptide repeat region
Pentapeptide	PF00805	172 - 210	6.7e-09	Pentapeptide repeats (8 copies)
Pentapeptide_4	PF13599	192 - 248	6.9e-06	Pentapeptide repeat region
Pentapeptide	PF00805	201 - 239	2.1e-12	Pentapeptide repeats (8 copies)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000859)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G55000 AT5G55000
fragaria_vesca	FvH4_1g28440 FvH4_3g41240 FvH4_3g41510
malus_domestica	MD03G1043800.v1.1 MD03G1043900.v1.1 MD03G1044000.v1.1 MD03G1047000.v1.1 MD11G1047200.v1.1 MD12G1008500.v1.1 MD14G1005800.v1.1
prunus_persica	Prupe.7G009900_v2.0.a1 Prupe.7G009900_v2.0.a1
pyrus_communis	pycom03g03380 pycom03g03390
rosa_chinensis	RchiOBHm_Chr3g0496431 RchiOBHm_Chr5g0074441 RchiOBHm_Chr5g0074451 RchiOBHm_Chr5g0074461
rosa_laevigata	RLG00000022527 RLG00000036444 RLG00000036448 RLG00000036477 RLG00000036478 RLG00000036479 RLG00000036481
rosa_multiflora	Rmu_sc0001467.1_g000023 Rmu_sc0001467.1_g000024 Rmu_sc0002461.1_g000009 Rmu_sc0004053.1_g000002 Rmu_sc0004053.1_g000004 Rmu_sc0011497.1_g000013 Rmu_sc0038981.1_g000001
rosa_roxburghii	Rroxscaffold_1G00007040 Rroxscaffold_1G00007060 Rroxscaffold_1G00007080 Rroxscaffold_6G00389210
rosa_rugosa	Rorug03G0282800 Rorug03G0282800 Rorug03G0282900 Rorug05G0428900 Rorug05G0431800 Rorug05G0431900 Rorug05G0432000 Rorug05G0432000
rosa_samantha	Rh3AG329000 Rh3BG364800 Rh3CG361900 Rh3DG364800 Rh5AG486100 Rh5AG489300 Rh5BG506300 Rh5BG506400 Rh5BG510300 Rh5CG530000 Rh5CG530100 Rh5CG534000 Rh5DG518900 Rh5DG522800 Rh5DG522900
rosa_wichuraiana	Rw3G028780 Rw5G045110 Rw5G045120 Rw5G045350 Rw5G045360 Rw5G045370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	130
AccB1I	GGYRCC	1 cut(s)	569
AccII	CGCG	2 cut(s)	379, 557
AciI	CCGC	4 cut(s)	275, 557, 625, 719
AcoI	YGGCCR	1 cut(s)	720
AcsI	RAATTY	4 cut(s)	242, 605, 664, 709
AcuI	CTGAAG	1 cut(s)	472
AcyI	GRCGYC	1 cut(s)	127
AfaI	GTAC	1 cut(s)	64
AfiI	CCNNNNNNNGG	2 cut(s)	154, 155
AgsI	TTSAA	4 cut(s)	102, 520, 543, 581
AluBI	AGCT	1 cut(s)	682
AluI	AGCT	1 cut(s)	682
Alw21I	GWGCWC	1 cut(s)	684
AoxI	GGCC	2 cut(s)	448, 720
ApeKI	GCWGC	1 cut(s)	110
ApoI	RAATTY	4 cut(s)	242, 605, 664, 709
AspLEI	GCGC	1 cut(s)	381
AspS9I	GGNCC	2 cut(s)	10, 145
AsuC2I	CCSGG	1 cut(s)	149
AsuII	TTCGAA	1 cut(s)	461
AvaII	GGWCC	2 cut(s)	10, 145
BanI	GGYRCC	1 cut(s)	569
BanII	GRGCYC	1 cut(s)	684
Bbv12I	GWGCWC	1 cut(s)	684
BbvCI	CCTCAGC	1 cut(s)	564
BbvI	GCAGC	1 cut(s)	97
BccI	CCATC	4 cut(s)	147, 173, 212, 581
BclI	TGATCA	1 cut(s)	343
BcnI	CCSGG	1 cut(s)	149
BfaI	CTAG	1 cut(s)	588
BfmI	CTRYAG	2 cut(s)	27, 675
BisI	GCNGC	2 cut(s)	111, 720
BlsI	GCNGC	2 cut(s)	112, 721
Bme1390I	CCNGG	1 cut(s)	149
Bme18I	GGWCC	2 cut(s)	10, 145
BmgT120I	GGNCC	2 cut(s)	10, 145
BmiI	GGNNCC	2 cut(s)	147, 571
BmrFI	CCNGG	1 cut(s)	149
BmsI	GCATC	1 cut(s)	550
Bpu10I	CCTNAGC	1 cut(s)	564
Bpu14I	TTCGAA	1 cut(s)	461
BpuEI	CTTGAG	1 cut(s)	23
BpuMI	CCSGG	1 cut(s)	149
BsaBI	GATNNNNATC	1 cut(s)	342
BsaHI	GRCGYC	1 cut(s)	127
BsaJI	CCNNGG	1 cut(s)	685
Bsc4I	CCNNNNNNNGG	2 cut(s)	154, 155
Bse1I	ACTGG	2 cut(s)	212, 723
Bse3DI	GCAATG	1 cut(s)	120
Bse8I	GATNNNNATC	1 cut(s)	342
BseDI	CCNNGG	1 cut(s)	685
BseGI	GGATG	5 cut(s)	184, 198, 223, 343, 565
BseJI	GATNNNNATC	1 cut(s)	342
BseLI	CCNNNNNNNGG	2 cut(s)	154, 155
BseMI	GCAATG	1 cut(s)	120
BseMII	CTCAG	2 cut(s)	494, 555
BseNI	ACTGG	2 cut(s)	212, 723
BseXI	GCAGC	1 cut(s)	97
BsgI	GTGCAG	1 cut(s)	387
Bsh1236I	CGCG	2 cut(s)	379, 557
BshFI	GGCC	2 cut(s)	450, 722
BshNI	GGYRCC	1 cut(s)	569
BsiHKAI	GWGCWC	1 cut(s)	684
BsiSI	CCGG	4 cut(s)	149, 236, 424, 447
BslI	CCNNNNNNNGG	2 cut(s)	154, 155
BsnI	GGCC	2 cut(s)	450, 722
Bsp119I	TTCGAA	1 cut(s)	461
Bsp1286I	GDGCHC	1 cut(s)	684
Bsp1407I	TGTACA	1 cut(s)	62
Bsp143I	GATC	4 cut(s)	42, 343, 529, 694
Bsp19I	CCATGG	1 cut(s)	685
BspACI	CCGC	4 cut(s)	275, 557, 625, 719
BspANI	GGCC	2 cut(s)	450, 722
BspCNI	CTCAG	2 cut(s)	495, 556
BspFNI	CGCG	2 cut(s)	379, 557
BspLI	GGNNCC	2 cut(s)	147, 571
BspMAI	CTGCAG	1 cut(s)	679
BspT104I	TTCGAA	1 cut(s)	461
BspT107I	GGYRCC	1 cut(s)	569
BsrDI	GCAATG	1 cut(s)	120
BsrGI	TGTACA	1 cut(s)	62
BsrI	ACTGG	2 cut(s)	212, 723
BssECI	CCNNGG	1 cut(s)	685
BssMI	GATC	4 cut(s)	42, 343, 529, 694
BssNI	GRCGYC	1 cut(s)	127
BssT1I	CCWWGG	1 cut(s)	685
Bst4CI	ACNGT	2 cut(s)	31, 70
BstACI	GRCGYC	1 cut(s)	127
BstAUI	TGTACA	1 cut(s)	62
BstBI	TTCGAA	1 cut(s)	461
BstC8I	GCNNGC	1 cut(s)	108
BstDEI	CTNAG	3 cut(s)	299, 503, 564
BstDSI	CCRYGG	1 cut(s)	685
BstF5I	GGATG	5 cut(s)	184, 198, 223, 343, 565
BstFNI	CGCG	2 cut(s)	379, 557
BstHHI	GCGC	1 cut(s)	381
BstKTI	GATC	4 cut(s)	45, 346, 532, 697
BstMBI	GATC	4 cut(s)	42, 343, 529, 694
BstMWI	GCNNNNNNNGC	1 cut(s)	569
BstNSI	RCATGY	2 cut(s)	501, 587
BstSCI	CCNGG	1 cut(s)	147
BstSFI	CTRYAG	2 cut(s)	27, 675
BstUI	CGCG	2 cut(s)	379, 557
BstV1I	GCAGC	1 cut(s)	97
BsuRI	GGCC	2 cut(s)	450, 722
BtgI	CCRYGG	1 cut(s)	685
BtsCI	GGATG	5 cut(s)	184, 198, 223, 343, 565
BtsIMutI	CAGTG	1 cut(s)	351
Cac8I	GCNNGC	1 cut(s)	108
CfoI	GCGC	1 cut(s)	381
Cfr13I	GGNCC	2 cut(s)	10, 145
Csp6I	GTAC	1 cut(s)	63
CviAII	CATG	6 cut(s)	131, 328, 399, 498, 584, 686
CviJI	RGCY	6 cut(s)	94, 317, 427, 450, 682, 722
CviKI_1	RGCY	6 cut(s)	94, 317, 427, 450, 682, 722
CviQI	GTAC	1 cut(s)	63
DdeI	CTNAG	3 cut(s)	299, 503, 564
DpnI	GATC	4 cut(s)	44, 345, 531, 696
DpnII	GATC	4 cut(s)	42, 343, 529, 694
EaeI	YGGCCR	1 cut(s)	720
EciI	GGCGGA	1 cut(s)	290
Ecl136II	GAGCTC	1 cut(s)	682
Eco130I	CCWWGG	1 cut(s)	685
Eco24I	GRGCYC	1 cut(s)	684
Eco32I	GATATC	2 cut(s)	388, 672
Eco47I	GGWCC	2 cut(s)	10, 145
Eco53kI	GAGCTC	1 cut(s)	682
Eco57I	CTGAAG	1 cut(s)	472
EcoICRI	GAGCTC	1 cut(s)	682
EcoO109I	RGGNCCY	2 cut(s)	10, 145
EcoRV	GATATC	2 cut(s)	388, 672
EcoT14I	CCWWGG	1 cut(s)	685
EcoT22I	ATGCAT	1 cut(s)	499
EcoT38I	GRGCYC	1 cut(s)	684
ErhI	CCWWGG	1 cut(s)	685
FaeI	CATG	6 cut(s)	134, 331, 402, 501, 587, 689
FalI	AAGNNNNNCTT	1 cut(s)	725
FatI	CATG	6 cut(s)	130, 327, 398, 497, 583, 685
FbaI	TGATCA	1 cut(s)	343
Fnu4HI	GCNGC	2 cut(s)	111, 720
FokI	GGATG	5 cut(s)	185, 191, 230, 350, 572
FriOI	GRGCYC	1 cut(s)	684
Fsp4HI	GCNGC	2 cut(s)	111, 720
FspBI	CTAG	1 cut(s)	588
GlaI	GCGC	1 cut(s)	380
GluI	GCNGC	2 cut(s)	111, 720
HaeIII	GGCC	2 cut(s)	450, 722
HapII	CCGG	4 cut(s)	149, 236, 424, 447
HhaI	GCGC	1 cut(s)	381
Hin1I	GRCGYC	1 cut(s)	127
Hin1II	CATG	6 cut(s)	134, 331, 402, 501, 587, 689
Hin6I	GCGC	1 cut(s)	379
HinP1I	GCGC	1 cut(s)	379
HincII	GTYRAC	1 cut(s)	439
HindII	GTYRAC	1 cut(s)	439
HinfI	GANTC	3 cut(s)	420, 619, 748
HpaI	GTTAAC	1 cut(s)	439
HpaII	CCGG	4 cut(s)	149, 236, 424, 447
Hpy166II	GTNNAC	2 cut(s)	125, 439
Hpy188I	TCNGA	4 cut(s)	47, 259, 504, 604
Hpy188III	TCNNGA	3 cut(s)	40, 143, 728
Hpy8I	GTNNAC	2 cut(s)	125, 439
HpyAV	CCTTC	1 cut(s)	328
HpyCH4III	ACNGT	2 cut(s)	31, 70
HpyCH4IV	ACGT	1 cut(s)	127
HpyCH4V	TGCA	6 cut(s)	113, 368, 497, 595, 677, 707
HpyF10VI	GCNNNNNNNGC	1 cut(s)	569
HpyF3I	CTNAG	3 cut(s)	299, 503, 564
HpySE526I	ACGT	1 cut(s)	127
Hsp92I	GRCGYC	1 cut(s)	127
Hsp92II	CATG	6 cut(s)	134, 331, 402, 501, 587, 689
HspAI	GCGC	1 cut(s)	379
Ksp22I	TGATCA	1 cut(s)	343
KspAI	GTTAAC	1 cut(s)	439
Kzo9I	GATC	4 cut(s)	42, 343, 529, 694
LmnI	GCTCC	1 cut(s)	687
Lsp1109I	GCAGC	1 cut(s)	97
LweI	GCATC	1 cut(s)	550
MaeI	CTAG	1 cut(s)	588
MaeII	ACGT	1 cut(s)	127
MalI	GATC	4 cut(s)	44, 345, 531, 696
MboI	GATC	4 cut(s)	42, 343, 529, 694
MboII	GAAGA	2 cut(s)	251, 524
MhlI	GDGCHC	1 cut(s)	684
MluCI	AATT	5 cut(s)	97, 242, 605, 664, 709
MnlI	CCTC	9 cut(s)	45, 241, 265, 324, 425, 452, 530, 559, 709
Mph1103I	ATGCAT	1 cut(s)	499
MseI	TTAA	6 cut(s)	15, 204, 212, 438, 453, 549
MslI	CAYNNNNRTG	1 cut(s)	558
MspI	CCGG	4 cut(s)	149, 236, 424, 447
MspR9I	CCNGG	1 cut(s)	149
MvnI	CGCG	2 cut(s)	379, 557
MwoI	GCNNNNNNNGC	1 cut(s)	569
NciI	CCSGG	1 cut(s)	149
NcoI	CCATGG	1 cut(s)	685
NdeII	GATC	4 cut(s)	42, 343, 529, 694
NlaIII	CATG	6 cut(s)	134, 331, 402, 501, 587, 689
NlaIV	GGNNCC	2 cut(s)	147, 571
NmeAIII	GCCGAG	1 cut(s)	64
NsiI	ATGCAT	1 cut(s)	499
NspI	RCATGY	2 cut(s)	501, 587
NspV	TTCGAA	1 cut(s)	461
PfeI	GAWTC	2 cut(s)	420, 619
PkrI	GCNGC	2 cut(s)	112, 721
PpuMI	RGGWCCY	2 cut(s)	10, 145
Psp124BI	GAGCTC	1 cut(s)	684
Psp5II	RGGWCCY	2 cut(s)	10, 145
PspN4I	GGNNCC	2 cut(s)	147, 571
PspPI	GGNCC	2 cut(s)	10, 145
PspPPI	RGGWCCY	2 cut(s)	10, 145
PstI	CTGCAG	1 cut(s)	679
RsaI	GTAC	1 cut(s)	64
RsaNI	GTAC	1 cut(s)	63
RseI	CAYNNNNRTG	1 cut(s)	558
SacI	GAGCTC	1 cut(s)	684
SaqAI	TTAA	6 cut(s)	15, 204, 212, 438, 453, 549
SatI	GCNGC	2 cut(s)	111, 720
Sau3AI	GATC	4 cut(s)	42, 343, 529, 694
Sau96I	GGNCC	2 cut(s)	10, 145
ScrFI	CCNGG	1 cut(s)	149
SduI	GDGCHC	1 cut(s)	684
SetI	ASST	7 cut(s)	15, 130, 444, 459, 541, 654, 684
SfaNI	GCATC	1 cut(s)	550
SfcI	CTRYAG	2 cut(s)	27, 675
SfuI	TTCGAA	1 cut(s)	461
SinI	GGWCC	2 cut(s)	10, 145
SmiMI	CAYNNNNRTG	1 cut(s)	558
SmlI	CTYRAG	1 cut(s)	38
SmoI	CTYRAG	1 cut(s)	38
Sse9I	AATT	5 cut(s)	97, 242, 605, 664, 709
SsiI	CCGC	4 cut(s)	275, 557, 625, 719
SspI	AATATT	1 cut(s)	254
SspMI	CTAG	1 cut(s)	588
SstI	GAGCTC	1 cut(s)	684
StyD4I	CCNGG	1 cut(s)	147
StyI	CCWWGG	1 cut(s)	685
TaaI	ACNGT	2 cut(s)	31, 70
TaiI	ACGT	1 cut(s)	130
TaqI	TCGA	4 cut(s)	171, 430, 461, 535
TasI	AATT	5 cut(s)	97, 242, 605, 664, 709
TatI	WGTACW	1 cut(s)	62
TauI	GCSGC	1 cut(s)	722
TfiI	GAWTC	2 cut(s)	420, 619
Tru1I	TTAA	6 cut(s)	15, 204, 212, 438, 453, 549
Tru9I	TTAA	6 cut(s)	15, 204, 212, 438, 453, 549
TscAI	CASTG	1 cut(s)	358
TseI	GCWGC	1 cut(s)	110
TspDTI	ATGAA	1 cut(s)	157
TspRI	CASTG	1 cut(s)	358
VpaK11BI	GGWCC	2 cut(s)	10, 145
XapI	RAATTY	4 cut(s)	242, 605, 664, 709
XceI	RCATGY	2 cut(s)	501, 587
XspI	CTAG	1 cut(s)	588
ZraI	GACGTC	1 cut(s)	128
Zsp2I	ATGCAT	1 cut(s)	499

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.