Rh5AG489300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000859
Physical Location & Seq
Forward (+)
83408784 .. 83417931
9148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG489300.1

Sequence Viewer

Length: 651 bp
ATGGGGCCAATGGCAACCGCTCTATTCCATCCTCCAATTAGCGGCCTTGCTCATTCTTTTGGGCCTGAGGCAATCCAAGCTCGCATAATCTGTGGCTCTGAAGCTCTCCAAAATTCCAGGACAAAGAAGTTGGGTGCAGCCACTGCAGCAGATCTAACACGCACCGATGTTATCAAGTTCATACATAATGAAACGAGCTACATGAAGAGCTGGCATAATTCCCCTCGACGGTTGAAGTTTAAAGGTGTAAACCTTTCTGGCCTTGATCTTTCTGGTTTGGACTTATCAAAGAGTCGATCATGTTTCAATCATGTTGATGCTGAAGGTGGAGACTTCATCTCTGCGTGTATGAAAAATGTGTGCTTTTCAAATGCAAATCCTTCAAAGTCGTGTTTCAATCATGTTAATGCTGAAGGTGGGGAATTTAGCCATGCATGTTTGAAAAAAGTGTGCTTTTCAAATGCAAATCTTACACAGTCCTATCTCAGTCATGTCGATGCTGAAGGTGCAGACTTTAACAAAGCGAATTTGCAAGGAACTTTATTTACTTGTACAAACCTTCGAGGAGCTTCTGTAGTCGGTGTTAAAAATAGCTCTTCATCTTTCCGATCAAAAACCTTTGCTCAAGCTGTATGGAATGTGTGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

216

Amino Acids

23.33

Weight (kDa)

9.17

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pentapeptide_4 PF13599 141 - 207 6.4e-07 Pentapeptide repeat region
Pentapeptide PF00805 161 - 195 2e-06 Pentapeptide repeats (8 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 20
AciI CCGC 2 cut(s) 18, 42
AcsI RAATTY 3 cut(s) 112, 422, 526
AcuI CTGAAG 4 cut(s) 120, 342, 432, 522
AfaI GTAC 1 cut(s) 553
AfiI CCNNNNNNNGG 2 cut(s) 41, 228
AgsI TTSAA 7 cut(s) 235, 307, 369, 384, 397, 442, 459
AjnI CCWGG 1 cut(s) 116
AluBI AGCT 7 cut(s) 80, 104, 198, 210, 569, 594, 629
AluI AGCT 7 cut(s) 80, 104, 198, 210, 569, 594, 629
Alw26I GTCTC 1 cut(s) 324
AlwNI CAGNNNCTG 1 cut(s) 143
AoxI GGCC 4 cut(s) 5, 43, 62, 259
ApeKI GCWGC 2 cut(s) 137, 146
ApoI RAATTY 3 cut(s) 112, 422, 526
AspS9I GGNCC 2 cut(s) 5, 62
AxyI CCTNAGG 1 cut(s) 66
BbvI GCAGC 2 cut(s) 149, 158
BccI CCATC 1 cut(s) 36
BciT130I CCWGG 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 324
BfmI CTRYAG 2 cut(s) 144, 573
BglII AGATCT 1 cut(s) 151
BisI GCNGC 3 cut(s) 43, 138, 147
BlsI GCNGC 3 cut(s) 44, 139, 148
Bme1390I CCNGG 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 5, 62
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 118
BmsI GCATC 2 cut(s) 307, 487
BpuEI CTTGAG 1 cut(s) 609
BsaXI ACNNNNNCTCC 2 cut(s) 558, 588
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 228
Bse21I CCTNAGG 1 cut(s) 66
BseBI CCWGG 1 cut(s) 118
BseGI GGATG 1 cut(s) 28
BseLI CCNNNNNNNGG 2 cut(s) 41, 228
BseMII CTCAG 2 cut(s) 57, 499
BseRI GAGGAG 1 cut(s) 579
BseXI GCAGC 2 cut(s) 149, 158
BsgI GTGCAG 2 cut(s) 156, 528
BshFI GGCC 4 cut(s) 7, 45, 64, 261
BslI CCNNNNNNNGG 2 cut(s) 41, 228
BsmAI GTCTC 1 cut(s) 324
BsnI GGCC 4 cut(s) 7, 45, 64, 261
Bsp1407I TGTACA 1 cut(s) 551
Bsp143I GATC 4 cut(s) 151, 265, 296, 608
BspACI CCGC 2 cut(s) 18, 42
BspANI GGCC 4 cut(s) 7, 45, 64, 261
BspCNI CTCAG 2 cut(s) 58, 498
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 148
BspQI GCTCTTC 2 cut(s) 200, 601
BsrBI CCGCTC 1 cut(s) 20
BsrGI TGTACA 1 cut(s) 551
BssMI GATC 4 cut(s) 151, 265, 296, 608
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 2 cut(s) 231, 477
Bst6I CTCTTC 2 cut(s) 200, 601
BstAPI GCANNNNNTGC 1 cut(s) 143
BstAUI TGTACA 1 cut(s) 551
BstC8I GCNNGC 2 cut(s) 82, 212
BstDEI CTNAG 2 cut(s) 66, 485
BstF5I GGATG 1 cut(s) 28
BstKTI GATC 4 cut(s) 154, 268, 299, 611
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 4 cut(s) 151, 265, 296, 608
BstMWI GCNNNNNNNGC 4 cut(s) 77, 143, 146, 506
BstNI CCWGG 1 cut(s) 118
BstNSI RCATGY 1 cut(s) 438
BstSCI CCNGG 1 cut(s) 116
BstSFI CTRYAG 2 cut(s) 144, 573
BstV1I GCAGC 2 cut(s) 149, 158
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
Bsu36I CCTNAGG 1 cut(s) 66
BsuRI GGCC 4 cut(s) 7, 45, 64, 261
BtsCI GGATG 1 cut(s) 28
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 1 cut(s) 141
Cac8I GCNNGC 2 cut(s) 82, 212
CaiI CAGNNNCTG 1 cut(s) 143
Cfr13I GGNCC 2 cut(s) 5, 62
Csp6I GTAC 1 cut(s) 552
CviAII CATG 7 cut(s) 202, 300, 311, 401, 431, 435, 491
CviQI GTAC 1 cut(s) 552
DdeI CTNAG 2 cut(s) 66, 485
DpnI GATC 4 cut(s) 153, 267, 298, 610
DpnII GATC 4 cut(s) 151, 265, 296, 608
DraI TTTAAA 1 cut(s) 241
Eam1104I CTCTTC 2 cut(s) 200, 601
EarI CTCTTC 2 cut(s) 200, 601
Eco57I CTGAAG 4 cut(s) 120, 342, 432, 522
Eco81I CCTNAGG 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 116
EcoT22I ATGCAT 1 cut(s) 436
FaeI CATG 7 cut(s) 205, 303, 314, 404, 434, 438, 494
FatI CATG 7 cut(s) 201, 299, 310, 400, 430, 434, 490
Fnu4HI GCNGC 3 cut(s) 43, 138, 147
FokI GGATG 1 cut(s) 15
Fsp4HI GCNGC 3 cut(s) 43, 138, 147
GluI GCNGC 3 cut(s) 43, 138, 147
HaeIII GGCC 4 cut(s) 7, 45, 64, 261
Hin1II CATG 7 cut(s) 205, 303, 314, 404, 434, 438, 494
HinfI GANTC 1 cut(s) 292
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 2 cut(s) 100, 608
Hpy8I GTNNAC 1 cut(s) 250
Hpy99I CGWCG 1 cut(s) 231
HpyAV CCTTC 5 cut(s) 317, 390, 407, 497, 569
HpyCH4III ACNGT 2 cut(s) 231, 477
HpyCH4V TGCA 7 cut(s) 137, 146, 374, 434, 464, 509, 532
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 143, 146, 506
HpyF3I CTNAG 2 cut(s) 66, 485
Hsp92II CATG 7 cut(s) 205, 303, 314, 404, 434, 438, 494
Kzo9I GATC 4 cut(s) 151, 265, 296, 608
LguI GCTCTTC 2 cut(s) 200, 601
LmnI GCTCC 1 cut(s) 566
LpnPI CCDG 6 cut(s) 78, 103, 130, 196, 243, 258
Lsp1109I GCAGC 2 cut(s) 149, 158
LweI GCATC 2 cut(s) 307, 487
MalI GATC 4 cut(s) 153, 267, 298, 610
MbiI CCGCTC 1 cut(s) 20
MboI GATC 4 cut(s) 151, 265, 296, 608
MboII GAAGA 2 cut(s) 217, 588
MflI RGATCY 1 cut(s) 151
MluCI AATT 5 cut(s) 36, 112, 217, 422, 526
MlyI GAGTC 1 cut(s) 301
MnlI CCTC 4 cut(s) 42, 61, 234, 557
Mph1103I ATGCAT 1 cut(s) 436
MseI TTAA 4 cut(s) 240, 405, 516, 585
MslI CAYNNNNRTG 3 cut(s) 315, 405, 495
MspR9I CCNGG 1 cut(s) 118
MvaI CCWGG 1 cut(s) 118
MwoI GCNNNNNNNGC 4 cut(s) 77, 143, 146, 506
NdeII GATC 4 cut(s) 151, 265, 296, 608
NlaIII CATG 7 cut(s) 205, 303, 314, 404, 434, 438, 494
NlaIV GGNNCC 1 cut(s) 6
NsiI ATGCAT 1 cut(s) 436
NspI RCATGY 1 cut(s) 438
PciSI GCTCTTC 2 cut(s) 200, 601
PfoI TCCNGGA 1 cut(s) 116
PkrI GCNGC 3 cut(s) 44, 139, 148
PleI GAGTC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 300
Psp6I CCWGG 1 cut(s) 116
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 5, 62
PsrI GAACNNNNNNTAC 2 cut(s) 529, 561
PstI CTGCAG 1 cut(s) 148
PstNI CAGNNNCTG 1 cut(s) 143
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 1 cut(s) 553
RsaNI GTAC 1 cut(s) 552
RseI CAYNNNNRTG 3 cut(s) 315, 405, 495
SapI GCTCTTC 2 cut(s) 200, 601
SaqAI TTAA 4 cut(s) 240, 405, 516, 585
SatI GCNGC 3 cut(s) 43, 138, 147
Sau3AI GATC 4 cut(s) 151, 265, 296, 608
Sau96I GGNCC 2 cut(s) 5, 62
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 1 cut(s) 118
SfaNI GCATC 2 cut(s) 307, 487
SfcI CTRYAG 2 cut(s) 144, 573
SmiMI CAYNNNNRTG 3 cut(s) 315, 405, 495
SmlI CTYRAG 1 cut(s) 624
SmoI CTYRAG 1 cut(s) 624
Sse9I AATT 5 cut(s) 36, 112, 217, 422, 526
SsiI CCGC 2 cut(s) 18, 42
StyD4I CCNGG 1 cut(s) 116
TaaI ACNGT 2 cut(s) 231, 477
TaqI TCGA 4 cut(s) 226, 295, 495, 562
TasI AATT 5 cut(s) 36, 112, 217, 422, 526
TatI WGTACW 1 cut(s) 551
TauI GCSGC 1 cut(s) 45
Tru1I TTAA 4 cut(s) 240, 405, 516, 585
Tru9I TTAA 4 cut(s) 240, 405, 516, 585
TscAI CASTG 1 cut(s) 148
TseI GCWGC 2 cut(s) 137, 146
TspDTI ATGAA 6 cut(s) 169, 204, 218, 325, 365, 588
TspRI CASTG 1 cut(s) 148
XapI RAATTY 3 cut(s) 112, 422, 526
XceI RCATGY 1 cut(s) 438
Zsp2I ATGCAT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.