pycom03g03380

FH protein interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000859
Physical Location & Seq
Reverse (-)
2690617 .. 2691342
726 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g03380.1

Sequence Viewer

Length: 726 bp
ATGCCAGAGGTCTATGCTTCTTCATCCTTGGTTCACCTCAATGTGGGAGGAAAGAAGTTATGCACCACCATCGGTACTCTAACTCAGCGTCAACCCGACTCCATGCTTGCTGCAATGTTCAGCAGTCGACACTCTTTGCGCAAAGATAAGAAGGGATACGTTTTCATCGATCGGGATGGCCAACATTTCCGGCACATTCTTAATTGGTTAAGAGACGGCGAGATTCCTAAATTAGAAGATTCTCAATATGCAGAACTTGTTAAGGAGGAAAAATACTATCTACTACTTGATTTAGTTGATGGAATACTTGATGTCCTTGATAGTAAGGAAGATACTGCGTCGGATGCAGATTTTACACGCACCGACGTGATCAAGTACATACATTCACAGAAGTCGTGTAGATGCTCGTCTAATAAGAATTATTTTCAAGGGGTTAAACTTTCTGGCCATAATCTTTCAAAATTGGACCTGTCAGAAGTTAACTTCAGCTCTGCATGTTTGAGAAATGTGAGTTTTTCAGGTGCAAATCTTCAGTCTGCTAGTTTCAAGCATGTGGATGCCGAAGGTGCTACTTTTGACGATGCCAATTTGTGTGGTTCTACTTTTTATGGAGCAAATCTACGAGGAGCGTCTTTGTGGGTGCAAAAAACTTCGATCTCTACGATCTCTCTGGGGCATCTGGCGTCGAATATTGTAGGCTCTGAACGTTATCCTCGACAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB_2 Pentapeptide

Protein Analysis

242

Amino Acids

26.8

Weight (kDa)

7.07

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB_2 PF02214 11 - 98 3.2e-18 BTB/POZ domain
Pentapeptide PF00805 142 - 174 3.8e-07 Pentapeptide repeats (8 copies)
Pentapeptide_4 PF13599 142 - 211 7.1e-10 Pentapeptide repeat region
Pentapeptide_3 PF13576 168 - 210 1.6e-06 Pentapeptide repeats (9 copies)
Pentapeptide PF00805 175 - 213 5.1e-09 Pentapeptide repeats (8 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 140
AccI GTMKAC 1 cut(s) 127
AclI AACGTT 1 cut(s) 706
AcoI YGGCCR 2 cut(s) 178, 445
AcuI CTGAAG 2 cut(s) 469, 515
AcyI GRCGYC 1 cut(s) 683
AfaI GTAC 2 cut(s) 76, 377
AfiI CCNNNNNNNGG 1 cut(s) 43
AgsI TTSAA 3 cut(s) 428, 459, 547
AjiI CACGTC 1 cut(s) 367
AleI CACNNNNGTG 1 cut(s) 365
AluBI AGCT 1 cut(s) 489
AluI AGCT 1 cut(s) 489
Alw26I GTCTC 1 cut(s) 207
AoxI GGCC 2 cut(s) 178, 445
ApeKI GCWGC 1 cut(s) 110
ArsI GACNNNNNNTTYG 2 cut(s) 518, 550
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 466
AsuHPI GGTGA 1 cut(s) 26
AvaII GGWCC 1 cut(s) 466
BalI TGGCCA 2 cut(s) 180, 447
BbvI GCAGC 1 cut(s) 97
BccI CCATC 3 cut(s) 77, 170, 293
BceAI ACGGC 1 cut(s) 232
BcgI CGANNNNNNTGC 2 cut(s) 52, 86
BciVI GTATCC 1 cut(s) 149
BclI TGATCA 1 cut(s) 369
BcoDI GTCTC 1 cut(s) 207
BfaI CTAG 1 cut(s) 540
BfuI GTATCC 1 cut(s) 149
BisI GCNGC 1 cut(s) 111
BlsI GCNGC 1 cut(s) 112
Bme18I GGWCC 1 cut(s) 466
BmgBI CACGTC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 466
BmsI GCATC 5 cut(s) 334, 392, 547, 571, 685
Bsa29I ATCGAT 1 cut(s) 168
BsaHI GRCGYC 1 cut(s) 683
BsaJI CCNNGG 1 cut(s) 27
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse3DI GCAATG 1 cut(s) 120
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 4 cut(s) 23, 181, 349, 562
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 1 cut(s) 98
BseRI GAGGAG 1 cut(s) 639
BseXI GCAGC 1 cut(s) 97
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 2 cut(s) 180, 447
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 172
BsiSI CCGG 1 cut(s) 190
BslI CCNNNNNNNGG 1 cut(s) 43
BsmAI GTCTC 1 cut(s) 207
BsmBI CGTCTC 1 cut(s) 207
BsnI GGCC 2 cut(s) 180, 447
Bsp143I GATC 4 cut(s) 169, 369, 654, 663
BspANI GGCC 2 cut(s) 180, 447
BspCNI CTCAG 1 cut(s) 97
BspDI ATCGAT 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 120
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 4 cut(s) 169, 369, 654, 663
BssNI GRCGYC 1 cut(s) 683
BssT1I CCWWGG 1 cut(s) 27
Bst4CI ACNGT 1 cut(s) 720
BstACI GRCGYC 1 cut(s) 683
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 1 cut(s) 84
BstF5I GGATG 4 cut(s) 23, 181, 349, 562
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 4 cut(s) 172, 372, 657, 666
BstMAI GTCTC 1 cut(s) 207
BstMBI GATC 4 cut(s) 169, 369, 654, 663
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 2 cut(s) 344, 566
BstNSI RCATGY 2 cut(s) 498, 554
BstV1I GCAGC 1 cut(s) 97
Bsu15I ATCGAT 1 cut(s) 168
BsuI GTATCC 1 cut(s) 149
BsuRI GGCC 2 cut(s) 180, 447
BsuTUI ATCGAT 1 cut(s) 168
BtrI CACGTC 1 cut(s) 367
BtsCI GGATG 4 cut(s) 23, 181, 349, 562
BtsIMutI CAGTG 1 cut(s) 725
Cac8I GCNNGC 1 cut(s) 108
CfoI GCGC 1 cut(s) 141
Cfr13I GGNCC 1 cut(s) 466
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 4 cut(s) 77, 327, 618, 672
Csp6I GTAC 2 cut(s) 75, 376
CspCI CAANNNNNGTGG 2 cut(s) 574, 609
CviAII CATG 3 cut(s) 103, 495, 551
CviJI RGCY 4 cut(s) 180, 447, 489, 699
CviKI_1 RGCY 4 cut(s) 180, 447, 489, 699
CviQI GTAC 2 cut(s) 75, 376
DdeI CTNAG 1 cut(s) 84
DpnI GATC 4 cut(s) 171, 371, 656, 665
DpnII GATC 4 cut(s) 169, 369, 654, 663
EaeI YGGCCR 2 cut(s) 178, 445
Eco130I CCWWGG 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 466
Eco57I CTGAAG 2 cut(s) 469, 515
EcoT14I CCWWGG 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 27
Esp3I CGTCTC 1 cut(s) 207
FaeI CATG 3 cut(s) 106, 498, 554
FaiI YATR 9 cut(s) 15, 61, 104, 249, 380, 450, 496, 552, 609
FatI CATG 3 cut(s) 102, 494, 550
FbaI TGATCA 1 cut(s) 369
FblI GTMKAC 1 cut(s) 127
Fnu4HI GCNGC 1 cut(s) 111
FokI GGATG 4 cut(s) 10, 188, 356, 569
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 1 cut(s) 540
FspI TGCGCA 1 cut(s) 140
GlaI GCGC 1 cut(s) 140
GluI GCNGC 1 cut(s) 111
HaeIII GGCC 2 cut(s) 180, 447
HapII CCGG 1 cut(s) 190
HgaI GACGC 4 cut(s) 77, 327, 618, 672
HhaI GCGC 1 cut(s) 141
Hin1I GRCGYC 1 cut(s) 683
Hin1II CATG 3 cut(s) 106, 498, 554
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HincII GTYRAC 3 cut(s) 92, 128, 481
HindII GTYRAC 3 cut(s) 92, 128, 481
HinfI GANTC 3 cut(s) 98, 223, 239
HpaI GTTAAC 1 cut(s) 481
HpaII CCGG 1 cut(s) 190
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 4 cut(s) 34, 92, 128, 481
Hpy188I TCNGA 3 cut(s) 343, 475, 703
Hpy188III TCNNGA 1 cut(s) 173
Hpy8I GTNNAC 4 cut(s) 34, 92, 128, 481
Hpy99I CGWCG 3 cut(s) 343, 368, 688
HpyAV CCTTC 2 cut(s) 145, 557
HpyCH4III ACNGT 1 cut(s) 720
HpyCH4IV ACGT 3 cut(s) 159, 366, 706
HpyCH4V TGCA 7 cut(s) 63, 113, 251, 347, 494, 524, 643
HpyF10VI GCNNNNNNNGC 2 cut(s) 344, 566
HpyF3I CTNAG 1 cut(s) 84
HpySE526I ACGT 3 cut(s) 159, 366, 706
Hsp92I GRCGYC 1 cut(s) 683
Hsp92II CATG 3 cut(s) 106, 498, 554
HspAI GCGC 1 cut(s) 139
Ksp22I TGATCA 1 cut(s) 369
KspAI GTTAAC 1 cut(s) 481
Kzo9I GATC 4 cut(s) 169, 369, 654, 663
LmnI GCTCC 2 cut(s) 611, 626
LpnPI CCDG 7 cut(s) 18, 203, 429, 482, 504, 656, 665
Lsp1109I GCAGC 1 cut(s) 97
LweI GCATC 5 cut(s) 334, 392, 547, 571, 685
MaeI CTAG 1 cut(s) 540
MaeII ACGT 3 cut(s) 159, 366, 706
MalI GATC 4 cut(s) 171, 371, 656, 665
MboI GATC 4 cut(s) 169, 369, 654, 663
MboII GAAGA 4 cut(s) 12, 248, 341, 521
MlsI TGGCCA 2 cut(s) 180, 447
MluCI AATT 5 cut(s) 202, 230, 418, 461, 586
MluNI TGGCCA 2 cut(s) 180, 447
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 5 cut(s) 41, 47, 259, 617, 723
Mox20I TGGCCA 2 cut(s) 180, 447
MscI TGGCCA 2 cut(s) 180, 447
MseI TTAA 5 cut(s) 201, 209, 261, 435, 480
MslI CAYNNNNRTG 3 cut(s) 39, 365, 555
Msp20I TGGCCA 2 cut(s) 180, 447
MspI CCGG 1 cut(s) 190
MwoI GCNNNNNNNGC 2 cut(s) 344, 566
NdeII GATC 4 cut(s) 169, 369, 654, 663
NlaIII CATG 3 cut(s) 106, 498, 554
NsbI TGCGCA 1 cut(s) 140
NspI RCATGY 2 cut(s) 498, 554
OliI CACNNNNGTG 1 cut(s) 365
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 659, 712
PfeI GAWTC 2 cut(s) 223, 239
PkrI GCNGC 1 cut(s) 112
Ple19I CGATCG 1 cut(s) 172
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
Psp1406I AACGTT 1 cut(s) 706
PspPI GGNCC 1 cut(s) 466
PvuI CGATCG 1 cut(s) 172
RsaI GTAC 2 cut(s) 76, 377
RsaNI GTAC 2 cut(s) 75, 376
RseI CAYNNNNRTG 3 cut(s) 39, 365, 555
SalI GTCGAC 1 cut(s) 126
SaqAI TTAA 5 cut(s) 201, 209, 261, 435, 480
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 4 cut(s) 169, 369, 654, 663
Sau96I GGNCC 1 cut(s) 466
SchI GAGTC 1 cut(s) 92
SetI ASST 9 cut(s) 12, 39, 162, 369, 471, 491, 523, 568, 709
SfaNI GCATC 5 cut(s) 334, 392, 547, 571, 685
SinI GGWCC 1 cut(s) 466
SmiMI CAYNNNNRTG 3 cut(s) 39, 365, 555
Sse9I AATT 5 cut(s) 202, 230, 418, 461, 586
SspI AATATT 1 cut(s) 691
SspMI CTAG 1 cut(s) 540
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 1 cut(s) 720
TaiI ACGT 3 cut(s) 162, 369, 709
TaqI TCGA 5 cut(s) 127, 168, 653, 686, 715
TasI AATT 5 cut(s) 202, 230, 418, 461, 586
TatI WGTACW 1 cut(s) 375
TfiI GAWTC 2 cut(s) 223, 239
Tru1I TTAA 5 cut(s) 201, 209, 261, 435, 480
Tru9I TTAA 5 cut(s) 201, 209, 261, 435, 480
TscAI CASTG 1 cut(s) 725
TseI GCWGC 1 cut(s) 110
TspDTI ATGAA 2 cut(s) 12, 154
TspRI CASTG 1 cut(s) 725
VpaK11BI GGWCC 1 cut(s) 466
XceI RCATGY 2 cut(s) 498, 554
XmiI GTMKAC 1 cut(s) 127
XspI CTAG 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.