Rorug03G0282800

Ubiquitin-conjugating enzyme E2 variant

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000859
Physical Location & Seq
Forward (+)
28420520 .. 28423523
3004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0282800.1

Sequence Viewer

Length: 465 bp
ATGGAGGGCAAGGGCAAAGGAAGCGAGAGATTTGGGGGAGCCATAGCCAATCTGACCGAGATGGCTCACAATTTGGAGTCACTCCAGAAGCTGCTCCTCAAGAAAGCTGTCTTTGTCGATGAAGAGACCTTTGGCAAAGCCTCACTTTGTTCTGAACAAGCTCGTACCATCAAGGTCCTTGAACAAAGGGTTGAAACTTTGGAACGAGAACTTGATGCTGCCATTACAGCTGCTGCACGTGCTCGTTCAGAAAAACGCCAGGCTGAGGCAGCAGAAAAGGCCGCTGAGTTACGTGCACAAGAAGTTACAAAAGAGCTTGAGAACACCTCAAAGGTATTTGAGCTGCACATGGAAGAGTTGCGTGCAAAGCAAGAAGAAATCTCTAAGCGTGATAAGGATATCAAACTTTTGGAAGCTATAATTCAGACACTTGGCGGAAAAGAAGTGCATAAAAGAACTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04624 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

154

Amino Acids

17.2

Weight (kDa)

6.37

Isoelectric Point (pI)

40.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 282, 435
AcvI CACGTG 1 cut(s) 239
AfaI GTAC 1 cut(s) 166
AfiI CCNNNNNNNGG 1 cut(s) 265
AgsI TTSAA 2 cut(s) 182, 194
AjnI CCWGG 1 cut(s) 258
AjuI GAANNNNNNNTTGG 2 cut(s) 114, 146
AluBI AGCT 7 cut(s) 91, 107, 161, 230, 316, 343, 416
AluI AGCT 7 cut(s) 91, 107, 161, 230, 316, 343, 416
Alw21I GWGCWC 2 cut(s) 244, 298
Alw26I GTCTC 1 cut(s) 119
Alw44I GTGCAC 1 cut(s) 294
AlwNI CAGNNNCTG 2 cut(s) 91, 233
AoxI GGCC 1 cut(s) 279
ApaLI GTGCAC 1 cut(s) 294
ApeKI GCWGC 6 cut(s) 91, 218, 230, 233, 269, 343
AspS9I GGNCC 1 cut(s) 175
AvaII GGWCC 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 298
BbrPI CACGTG 1 cut(s) 239
Bbv12I GWGCWC 2 cut(s) 244, 298
BbvCI CCTCAGC 1 cut(s) 264
BbvI GCAGC 6 cut(s) 78, 205, 217, 220, 281, 330
BccI CCATC 2 cut(s) 55, 176
BciT130I CCWGG 1 cut(s) 260
BcoDI GTCTC 1 cut(s) 119
BisI GCNGC 7 cut(s) 92, 219, 231, 234, 270, 282, 344
BlsI GCNGC 7 cut(s) 93, 220, 232, 235, 271, 283, 345
Bme1390I CCNGG 1 cut(s) 260
Bme18I GGWCC 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 175
BmiI GGNNCC 1 cut(s) 40
BmrFI CCNGG 1 cut(s) 260
BmsI GCATC 1 cut(s) 205
BplI GAGNNNNNCTC 2 cut(s) 311, 343
BpmI CTGGAG 1 cut(s) 68
Bpu10I CCTNAGC 1 cut(s) 264
BpuEI CTTGAG 2 cut(s) 83, 338
BsaAI YACGTR 2 cut(s) 239, 293
BsaI GGTCTC 1 cut(s) 119
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse1I ACTGG 1 cut(s) 463
BseBI CCWGG 1 cut(s) 260
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMII CTCAG 2 cut(s) 255, 276
BseNI ACTGG 1 cut(s) 463
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 6 cut(s) 78, 205, 217, 220, 281, 330
BsgI GTGCAG 2 cut(s) 219, 329
BshFI GGCC 1 cut(s) 281
BsiHKAI GWGCWC 2 cut(s) 244, 298
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 1 cut(s) 119
BsnI GGCC 1 cut(s) 281
Bso31I GGTCTC 1 cut(s) 119
Bsp1286I GDGCHC 2 cut(s) 244, 298
BspACI CCGC 2 cut(s) 282, 435
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 2 cut(s) 256, 277
BspLI GGNNCC 1 cut(s) 40
BspTNI GGTCTC 1 cut(s) 119
BsrI ACTGG 1 cut(s) 463
Bst2UI CCWGG 1 cut(s) 260
Bst6I CTCTTC 2 cut(s) 117, 348
BstBAI YACGTR 2 cut(s) 239, 293
BstC8I GCNNGC 1 cut(s) 363
BstDEI CTNAG 3 cut(s) 264, 285, 384
BstMAI GTCTC 1 cut(s) 119
BstMWI GCNNNNNNNGC 6 cut(s) 21, 227, 239, 269, 278, 367
BstNI CCWGG 1 cut(s) 260
BstSCI CCNGG 1 cut(s) 258
BstSLI GKGCMC 1 cut(s) 298
BstV1I GCAGC 6 cut(s) 78, 205, 217, 220, 281, 330
BsuRI GGCC 1 cut(s) 281
Cac8I GCNNGC 1 cut(s) 363
CaiI CAGNNNCTG 2 cut(s) 91, 233
Cfr13I GGNCC 1 cut(s) 175
Csp6I GTAC 1 cut(s) 165
CviAII CATG 1 cut(s) 349
CviQI GTAC 1 cut(s) 165
DdeI CTNAG 3 cut(s) 264, 285, 384
Eam1104I CTCTTC 2 cut(s) 117, 348
EarI CTCTTC 2 cut(s) 117, 348
EciI GGCGGA 1 cut(s) 450
Eco31I GGTCTC 1 cut(s) 119
Eco32I GATATC 1 cut(s) 400
Eco47I GGWCC 1 cut(s) 175
Eco72I CACGTG 1 cut(s) 239
EcoO109I RGGNCCY 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 258
EcoRV GATATC 1 cut(s) 400
FaeI CATG 1 cut(s) 352
FaiI YATR 4 cut(s) 44, 350, 419, 450
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FatI CATG 1 cut(s) 348
Fnu4HI GCNGC 7 cut(s) 92, 219, 231, 234, 270, 282, 344
Fsp4HI GCNGC 7 cut(s) 92, 219, 231, 234, 270, 282, 344
GluI GCNGC 7 cut(s) 92, 219, 231, 234, 270, 282, 344
GsuI CTGGAG 1 cut(s) 68
HaeIII GGCC 1 cut(s) 281
Hin1II CATG 1 cut(s) 352
HinfI GANTC 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 296
Hpy188I TCNGA 4 cut(s) 54, 154, 250, 426
Hpy188III TCNNGA 2 cut(s) 85, 100
Hpy8I GTNNAC 1 cut(s) 296
HpyCH4IV ACGT 2 cut(s) 238, 292
HpyCH4V TGCA 5 cut(s) 236, 296, 346, 365, 448
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 227, 239, 269, 278, 367
HpyF3I CTNAG 3 cut(s) 264, 285, 384
HpySE526I ACGT 2 cut(s) 238, 292
Hsp92II CATG 1 cut(s) 352
LmnI GCTCC 2 cut(s) 38, 99
LpnPI CCDG 4 cut(s) 98, 245, 272, 444
Lsp1109I GCAGC 6 cut(s) 78, 205, 217, 220, 281, 330
LweI GCATC 1 cut(s) 205
MaeII ACGT 2 cut(s) 238, 292
MaeIII GTNAC 3 cut(s) 78, 288, 304
MboII GAAGA 3 cut(s) 134, 365, 386
MhlI GDGCHC 2 cut(s) 244, 298
MluCI AATT 2 cut(s) 70, 420
MlyI GAGTC 1 cut(s) 86
MnlI CCTC 4 cut(s) 107, 151, 259, 337
MspA1I CMGCKG 2 cut(s) 230, 284
MspR9I CCNGG 1 cut(s) 260
MvaI CCWGG 1 cut(s) 260
MwoI GCNNNNNNNGC 6 cut(s) 21, 227, 239, 269, 278, 367
NlaIII CATG 1 cut(s) 352
NlaIV GGNNCC 1 cut(s) 40
NmuCI GTSAC 1 cut(s) 78
PkrI GCNGC 7 cut(s) 93, 220, 232, 235, 271, 283, 345
PleI GAGTC 1 cut(s) 85
PmaCI CACGTG 1 cut(s) 239
PmlI CACGTG 1 cut(s) 239
PpsI GAGTC 1 cut(s) 85
Ppu21I YACGTR 2 cut(s) 239, 293
PpuMI RGGWCCY 1 cut(s) 175
Psp5II RGGWCCY 1 cut(s) 175
Psp6I CCWGG 1 cut(s) 258
PspCI CACGTG 1 cut(s) 239
PspGI CCWGG 1 cut(s) 258
PspN4I GGNNCC 1 cut(s) 40
PspPI GGNCC 1 cut(s) 175
PspPPI RGGWCCY 1 cut(s) 175
PstNI CAGNNNCTG 2 cut(s) 91, 233
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 1 cut(s) 166
RsaNI GTAC 1 cut(s) 165
SatI GCNGC 7 cut(s) 92, 219, 231, 234, 270, 282, 344
Sau96I GGNCC 1 cut(s) 175
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 260
SduI GDGCHC 2 cut(s) 244, 298
SfaNI GCATC 1 cut(s) 205
SinI GGWCC 1 cut(s) 175
SmlI CTYRAG 2 cut(s) 98, 317
SmoI CTYRAG 2 cut(s) 98, 317
Sse9I AATT 2 cut(s) 70, 420
SsiI CCGC 2 cut(s) 282, 435
StyD4I CCNGG 1 cut(s) 258
TaiI ACGT 2 cut(s) 241, 295
TaqI TCGA 1 cut(s) 117
TaqII GACCGA 1 cut(s) 71
TasI AATT 2 cut(s) 70, 420
TauI GCSGC 1 cut(s) 284
TseFI GTSAC 1 cut(s) 78
TseI GCWGC 6 cut(s) 91, 218, 230, 233, 269, 343
Tsp45I GTSAC 1 cut(s) 78
TspDTI ATGAA 1 cut(s) 135
VneI GTGCAC 1 cut(s) 294
VpaK11BI GGWCC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.