MD11G1047200.v1.1

FH protein interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000859
Physical Location & Seq
Reverse (-)
4103202 .. 4103879
678 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1047200.v1.1.491

Sequence Viewer

Length: 678 bp
ATGGCAGATGGCTATGACTCTTCATCCTTGGTTCACCTCAATGTTGGAGGAAGAAAGTTTTCCACCACTGTTGGTACTCTAACTCAGCGCGAGCCTGATTCGATGCTAGCTGCAATGTTTAGTGGCCGTCACACTTTACGCCAAGATAAGAAGGGATATGTTTTTATCGATAGGGATGGTAAGCTTTTTCGGCACATCCTTAATTGGTTAAGAGACGGTGATATTCCTAAACTGGAAGATACCCAATATGCAGAACTTCTTAAGGAGGCAAAATACTATCAACTAGTTGGGTTGACAGATGAATTACAAGGTGAACTTCTGCATAGGAGCAAAGGTGAAAGTCTAAACTCAGATGTAACACGCGCTGATATCATGAAGTACTATATACGCGGGCAGCACATCAATCTTCGAGGTGTTAATCTTTCTGGCATTGATCTCTCTGGATTGAATTTATCGAAAGTCGACTTCAGCTATTTGTGCATGAGATATGTTCGTTTCTCAAATGCAGATATTCGGGATGCTAAGTTTAATTATGTAGATGCTGAGGGTGCTACTTTTGACAATGCCGATTTGTGGGATTCTACTTTTTATGGAGCAAATCTACGAGGAGCTTCTTTTGTGGGGAGTAAAGGGTACAGACTCTTTGGGGCATCTTGCGTGGAAGATTGTAGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB_2 Pentapeptide

Protein Analysis

226

Amino Acids

25.34

Weight (kDa)

6.21

Isoelectric Point (pI)

26.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB_2 PF02214 11 - 98 6.2e-21 BTB/POZ domain
Pentapeptide_4 PF13599 132 - 186 9.1e-06 Pentapeptide repeat region
Pentapeptide PF00805 135 - 173 1.5e-07 Pentapeptide repeats (8 copies)
Pentapeptide_3 PF13576 164 - 204 8.9e-07 Pentapeptide repeats (9 copies)
Pentapeptide PF00805 174 - 209 3.6e-06 Pentapeptide repeats (8 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 462
AccII CGCG 3 cut(s) 90, 363, 390
AciI CCGC 1 cut(s) 390
AcoI YGGCCR 1 cut(s) 124
AcsI RAATTY 1 cut(s) 448
AcuI CTGAAG 1 cut(s) 451
AfaI GTAC 3 cut(s) 76, 380, 635
AfiI CCNNNNNNNGG 1 cut(s) 573
AflII CTTAAG 1 cut(s) 260
AgsI TTSAA 1 cut(s) 448
AhlI ACTAGT 1 cut(s) 283
AluBI AGCT 5 cut(s) 110, 184, 471, 611, 672
AluI AGCT 5 cut(s) 110, 184, 471, 611, 672
Alw26I GTCTC 1 cut(s) 207
AoxI GGCC 1 cut(s) 124
ApeKI GCWGC 3 cut(s) 110, 394, 672
ApoI RAATTY 1 cut(s) 448
Asp700I GAANNNNTTC 1 cut(s) 58
AspLEI GCGC 2 cut(s) 90, 365
AsuHPI GGTGA 4 cut(s) 26, 230, 323, 347
AsuNHI GCTAGC 1 cut(s) 106
BbvCI CCTCAGC 1 cut(s) 543
BbvI GCAGC 3 cut(s) 97, 406, 659
BccI CCATC 2 cut(s) 2, 170
BceAI ACGGC 1 cut(s) 111
BcoDI GTCTC 1 cut(s) 207
BcuI ACTAGT 1 cut(s) 283
BfaI CTAG 2 cut(s) 107, 284
BfrI CTTAAG 1 cut(s) 260
BisI GCNGC 3 cut(s) 111, 395, 673
BlsI GCNGC 3 cut(s) 112, 396, 674
BmcAI AGTACT 1 cut(s) 380
BmsI GCATC 4 cut(s) 93, 508, 529, 659
BmtI GCTAGC 1 cut(s) 110
Bpu10I CCTNAGC 1 cut(s) 543
Bsa29I ATCGAT 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 27
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bsc4I CCNNNNNNNGG 1 cut(s) 573
Bse1I ACTGG 1 cut(s) 237
Bse3DI GCAATG 1 cut(s) 120
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 4 cut(s) 23, 181, 195, 523
BseLI CCNNNNNNNGG 1 cut(s) 573
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 3 cut(s) 98, 363, 534
BseNI ACTGG 1 cut(s) 237
BseRI GAGGAG 1 cut(s) 621
BseXI GCAGC 3 cut(s) 97, 406, 659
Bsh1236I CGCG 3 cut(s) 90, 363, 390
BshFI GGCC 1 cut(s) 126
BshVI ATCGAT 1 cut(s) 168
BslI CCNNNNNNNGG 1 cut(s) 573
BsmAI GTCTC 1 cut(s) 207
BsmBI CGTCTC 1 cut(s) 207
BsnI GGCC 1 cut(s) 126
Bsp143I GATC 1 cut(s) 433
BspACI CCGC 1 cut(s) 390
BspANI GGCC 1 cut(s) 126
BspCNI CTCAG 3 cut(s) 97, 362, 535
BspDI ATCGAT 1 cut(s) 168
BspFNI CGCG 3 cut(s) 90, 363, 390
BspHI TCATGA 1 cut(s) 372
BspOI GCTAGC 1 cut(s) 110
BspTI CTTAAG 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 120
BsrI ACTGG 1 cut(s) 237
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 1 cut(s) 433
BssT1I CCWWGG 1 cut(s) 27
Bst4CI ACNGT 2 cut(s) 70, 218
Bst6I CTCTTC 1 cut(s) 25
BstAFI CTTAAG 1 cut(s) 260
BstC8I GCNNGC 3 cut(s) 92, 108, 392
BstDEI CTNAG 4 cut(s) 84, 349, 522, 543
BstF5I GGATG 4 cut(s) 23, 181, 195, 523
BstFNI CGCG 3 cut(s) 90, 363, 390
BstHHI GCGC 2 cut(s) 90, 365
BstKTI GATC 1 cut(s) 436
BstMAI GTCTC 1 cut(s) 207
BstMBI GATC 1 cut(s) 433
BstMWI GCNNNNNNNGC 3 cut(s) 190, 477, 548
BstUI CGCG 3 cut(s) 90, 363, 390
BstV1I GCAGC 3 cut(s) 97, 406, 659
Bsu15I ATCGAT 1 cut(s) 168
BsuRI GGCC 1 cut(s) 126
BsuTUI ATCGAT 1 cut(s) 168
BtsCI GGATG 4 cut(s) 23, 181, 195, 523
BtsIMutI CAGTG 1 cut(s) 66
Cac8I GCNNGC 3 cut(s) 92, 108, 392
CciI TCATGA 1 cut(s) 372
CfoI GCGC 2 cut(s) 90, 365
ClaI ATCGAT 1 cut(s) 168
Csp6I GTAC 3 cut(s) 75, 379, 634
CspCI CAANNNNNGTGG 2 cut(s) 52, 87
CviAII CATG 2 cut(s) 373, 481
CviJI RGCY 8 cut(s) 12, 94, 110, 126, 184, 471, 611, 672
CviKI_1 RGCY 8 cut(s) 12, 94, 110, 126, 184, 471, 611, 672
CviQI GTAC 3 cut(s) 75, 379, 634
DdeI CTNAG 4 cut(s) 84, 349, 522, 543
DpnI GATC 1 cut(s) 435
DpnII GATC 1 cut(s) 433
EaeI YGGCCR 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 25
EarI CTCTTC 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 27
Eco32I GATATC 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 451
EcoRV GATATC 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 27
Esp3I CGTCTC 1 cut(s) 207
FaeI CATG 2 cut(s) 376, 484
FalI AAGNNNNNCTT 2 cut(s) 300, 332
FatI CATG 2 cut(s) 372, 480
FauI CCCGC 1 cut(s) 383
FblI GTMKAC 1 cut(s) 462
Fnu4HI GCNGC 3 cut(s) 111, 395, 673
FokI GGATG 4 cut(s) 10, 182, 188, 530
Fsp4HI GCNGC 3 cut(s) 111, 395, 673
FspBI CTAG 2 cut(s) 107, 284
GlaI GCGC 2 cut(s) 89, 364
GluI GCNGC 3 cut(s) 111, 395, 673
HaeIII GGCC 1 cut(s) 126
HhaI GCGC 2 cut(s) 90, 365
Hin1II CATG 2 cut(s) 376, 484
Hin6I GCGC 2 cut(s) 88, 363
HinP1I GCGC 2 cut(s) 88, 363
HincII GTYRAC 2 cut(s) 294, 463
HindII GTYRAC 2 cut(s) 294, 463
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 4 cut(s) 17, 98, 578, 639
HphI GGTGA 4 cut(s) 26, 230, 323, 347
Hpy166II GTNNAC 4 cut(s) 34, 294, 314, 463
Hpy188I TCNGA 1 cut(s) 352
Hpy188III TCNNGA 3 cut(s) 373, 441, 515
Hpy8I GTNNAC 4 cut(s) 34, 294, 314, 463
HpyAV CCTTC 1 cut(s) 145
HpyCH4III ACNGT 2 cut(s) 70, 218
HpyCH4V TGCA 5 cut(s) 113, 251, 322, 480, 506
HpyF10VI GCNNNNNNNGC 3 cut(s) 190, 477, 548
HpyF3I CTNAG 4 cut(s) 84, 349, 522, 543
Hsp92II CATG 2 cut(s) 376, 484
HspAI GCGC 2 cut(s) 88, 363
Kzo9I GATC 1 cut(s) 433
LmnI GCTCC 3 cut(s) 327, 593, 608
LpnPI CCDG 4 cut(s) 108, 218, 411, 426
Lsp1109I GCAGC 3 cut(s) 97, 406, 659
LweI GCATC 4 cut(s) 93, 508, 529, 659
MaeI CTAG 2 cut(s) 107, 284
MaeIII GTNAC 2 cut(s) 128, 355
MalI GATC 1 cut(s) 435
MboI GATC 1 cut(s) 433
MboII GAAGA 5 cut(s) 12, 63, 248, 398, 674
MluCI AATT 4 cut(s) 202, 302, 448, 529
MlyI GAGTC 2 cut(s) 11, 633
MmeI TCCRAC 1 cut(s) 25
MnlI CCTC 6 cut(s) 41, 47, 259, 404, 538, 599
MroXI GAANNNNTTC 1 cut(s) 58
MseI TTAA 5 cut(s) 201, 209, 261, 417, 528
MslI CAYNNNNRTG 1 cut(s) 39
MspCI CTTAAG 1 cut(s) 260
MvnI CGCG 3 cut(s) 90, 363, 390
MwoI GCNNNNNNNGC 3 cut(s) 190, 477, 548
NdeII GATC 1 cut(s) 433
NheI GCTAGC 1 cut(s) 106
NlaIII CATG 2 cut(s) 376, 484
NmuCI GTSAC 1 cut(s) 128
PagI TCATGA 1 cut(s) 372
PdmI GAANNNNTTC 1 cut(s) 58
PfeI GAWTC 2 cut(s) 98, 578
PkrI GCNGC 3 cut(s) 112, 396, 674
PleI GAGTC 2 cut(s) 11, 633
PpsI GAGTC 2 cut(s) 11, 633
RsaI GTAC 3 cut(s) 76, 380, 635
RsaNI GTAC 3 cut(s) 75, 379, 634
RseI CAYNNNNRTG 1 cut(s) 39
SalI GTCGAC 1 cut(s) 461
SaqAI TTAA 5 cut(s) 201, 209, 261, 417, 528
SatI GCNGC 3 cut(s) 111, 395, 673
Sau3AI GATC 1 cut(s) 433
ScaI AGTACT 1 cut(s) 380
SchI GAGTC 2 cut(s) 11, 633
SetI ASST 9 cut(s) 39, 112, 186, 313, 337, 415, 473, 613, 674
SfaNI GCATC 4 cut(s) 93, 508, 529, 659
SmiMI CAYNNNNRTG 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
SpeI ACTAGT 1 cut(s) 283
Sse9I AATT 4 cut(s) 202, 302, 448, 529
SsiI CCGC 1 cut(s) 390
SspMI CTAG 2 cut(s) 107, 284
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 2 cut(s) 70, 218
TaqI TCGA 5 cut(s) 101, 168, 409, 455, 462
TasI AATT 4 cut(s) 202, 302, 448, 529
TatI WGTACW 1 cut(s) 378
TfiI GAWTC 2 cut(s) 98, 578
Tru1I TTAA 5 cut(s) 201, 209, 261, 417, 528
Tru9I TTAA 5 cut(s) 201, 209, 261, 417, 528
TscAI CASTG 1 cut(s) 73
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 3 cut(s) 110, 394, 672
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 3 cut(s) 12, 315, 389
TspRI CASTG 1 cut(s) 73
Vha464I CTTAAG 1 cut(s) 260
XapI RAATTY 1 cut(s) 448
XmiI GTMKAC 1 cut(s) 462
XmnI GAANNNNTTC 1 cut(s) 58
XspI CTAG 2 cut(s) 107, 284
ZrmI AGTACT 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.