MD05G1207400.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000865
Physical Location & Seq
Reverse (-)
33813808 .. 33816620
2813 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1207400.v1.1.491

Sequence Viewer

Length: 2625 bp
ATGGCTGAAATTGCCGTACCAGTAGCGTTGTTTCTGGCGGACAAAGTGATAATGTTCCTCGACAAAAAATTCAGCCGATCGAGCTTTAATAATAATAATCCCCGCGATGATGCTGGAAGAGCCAGAAGCTGTTTAGAGAGGATGCGAGCATATCTCAGTGACAACTCATCAGCAGAACTGTCTGCTTACGATGGAGGGTCTCAACAGTTTCAAACTCGTGTAAAAGAGATCCGAAATATAGCTTATGAAATTGAAGACGTTCTTGACGAATTTCTGCTTCATGTCCCTCATCAATTCCACAACAACGACGTCTTTCAGAAGCTACACGATGCTGTGAAATTTCGCACGGTACAGAAAGCAAGCCGTGAGTTCTCGGACAGAATTAAAAATATTGACAAGAAGCTAAATTTTGTTTTCACTCTTGATAAAATTCCACAATTTCCGGGACGAGATCAAGAAAGTCGCCCAAGTTCCAGCACCACAAGACATGGAGATAGCACGGTGACTCATCATAACCAATTCCTTGAAGAAGAAGAAATGGTAGGGTTTGAGAAGCCTAAAGAAAAACTCATCAGTCAGTTGATGAAAGGAGATCCAAGGTTTCCGAATATCTCGCTTGTAGGTCCTGGTGGCTCAGGCAAAACCACTCTTTTGAAGAATGTCTTCCAGAGCAAAAAGGTCCAACGGTTTTTTGAATGCCATGCTTGGATTGATGTGCCGCGTGACTTGTGTCCCCGTAAACTTGATGAGCTCTTGCCCAACATGTTAAGCAAATTTGATCCAAAAGGCAAAAGGAAGGAATCCATTAATCATGAAGATCCAAAGGCGCAATTGGTAAGAGCTTTGAAGGGCAAGAAGTTTGTGGTCGTGTTAGATAATGTTTGGAGCAAACAAGATTTGGAACGCATTGTAAATGTTTTACCAAATGATTTACCTGGGACTGGGAGTAAAATAGTCATAACTACTCGTTACTCTGAAGTAGCGTCTTCTCATGCAAACTCTTCGTACATCCACAATATGAGCAATGTGTTGTCATGGGAACAAGCTTGGAATCTATTCTGCCGAAAGGCTTTTCACAAGAGCGAAGGAAAGTGTCCAACCCAGATTTTGGACTGGGCAGAGAAAATCGTCAAGAAGTGTGAAGGCTTGCCCCTTGCAATTTCAGCTGTTGGTACTTTGCTAGCAACGAAGCGACCAACTCCACTTGATTGGAAGAAGTTACATGACAGCCTTGGATCAAATGTTCCCATTATTACCCAAATATTGGAGCCCAGTTACAAGGACCTTCCAAGCCATCTTAAGACTTGTTTCTTGTACTTTGGCATGTTTCCTGAAGACTACTCCATTAGCCGTGAAAGGCTAATTCGCTTGTGGGTAGCTGAAGGATTTGTGATGCAGAACAGATCAAGAAAACTAATGGAGGAGGTTGCAGGAGGCTATCTGAATGATCTCATTGGAAGAAATTTAGTTCATGTGAGTTCAAGGGAAGTCGATGGAAGAGTCAGAAGCTGCCGTGTACTGAACCTCGTTCGCGAGTTCATCATTGAAAAAGCGGAGAATTTCATCACTGTCTCGTTGGCAGCAAACTGCAGAGGTACTGCTCCAGGTGAGAAAATTCGACACCTTTCTGTTCATGATGTTAGTAAGGGCAACAATTTTTCAAGTGGTACAGATTTGAGTTGTACTCGTACCATACTATTAAGTGGGCAGGGAAGTTATGATTCCGAGCTTGAAAGACTACTTAAAACCTTTAAATTTTTAAGGGTTTTAGATTTGCAAGGGATAAATTTGGACAACTTTCCCGATTCTGTTTTTGGTCTCACCCTCTTGAGGTACATAAGTCTAAGGCACACCGATATTAAAGAAGTGCCAAAGTCCATAAAGAAGCTTGGATTCTTGGAAACCTTAGACCTTAAGCACACAAAAGTGACCAATTTACCAAAACAGATTTATAAGCTCCGCAATTTGCACCACCTTTTAGTCTATCGCTATGATGTCACGAATTATGTGACATTTGAAGCTGCAAGAAGTCTCTCGACATTGGATGTACGATCAACGAGCGAGGAGGACTTTCTGGATTTGGATCATGGTGAATTTCGTCCTGACTTTCTGCAACGCCTATATTTGAAAGGGCGCCTAGAAAGGCTGCCAATGTGGATTTCCAAACTTCACAGCGTAGTAAAGATTGGTTTAAAGTGGTCAAAACTTCAGGCTAATGAAAAACCACTTGAGGCCCTTCAAGCTTTGCCTGAGCTGATGGAGCTCGATTTGGTTCAGTACCACACCGGTGCAGAGTTGAAATTCAAAGCTGAAACGTTCCACAAGCTAAAGATATTACATATTGAACAGTTTGATCAGCTAAATAAGATGGTAGTCGAGAACGGGGCAATGCCTAAGCTTGAGAAGCTAATCATGTCTAGATGTCAAAATTTGAGCTTACTTCCACTTGGAATGGAGCATCTTACGCATCTCGACGAACTGCTTCTATATGACATGCCTGATGAGTTCATCGCTAAGCTTGGGAAAGACAGTGAGGACCGTGCTCATGTTGAGCATATTCGAGTCATCCACTCTTTCCATCTCGGGAGCAACCAGTCCTTCACTGGATTTCAAAATCTTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

875

Amino Acids

100.2

Weight (kDa)

8.88

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 31 - 99 1.4e-08 Rx N-terminal domain
NB-ARC PF00931 184 - 361 3.5e-36 NB-ARC domain
WHD_DRP PF23559 442 - 514 5.9e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 580 - 688 1.8e-21 Leucine-rich repeat region
LRR_14 PF23598 677 - 826 7.6e-13 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1953
AatII GACGTC 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 2133
AccB7I CCANNNNNTGG 2 cut(s) 1108, 1264
AccII CGCG 3 cut(s) 105, 721, 1533
AciI CCGC 5 cut(s) 38, 103, 719, 1553, 1960
AclI AACGTT 1 cut(s) 2315
AclWI GGATC 6 cut(s) 223, 587, 773, 812, 1243, 2091
AcuI CTGAAG 4 cut(s) 996, 1353, 1401, 2192
AcyI GRCGYC 2 cut(s) 309, 2134
AfiI CCNNNNNNNGG 4 cut(s) 941, 1108, 1264, 1830
AflII CTTAAG 2 cut(s) 1298, 1913
AflIII ACRYGT 1 cut(s) 762
AgeI ACCGGT 1 cut(s) 2285
AjnI CCWGG 3 cut(s) 625, 934, 1603
AjuI GAANNNNNNNTTGG 2 cut(s) 2143, 2175
AleI CACNNNNGTG 2 cut(s) 1925, 2286
AloI GAACNNNNNNTCC 2 cut(s) 1227, 1259
Alw21I GWGCWC 3 cut(s) 753, 2265, 2545
Alw26I GTCTC 4 cut(s) 204, 1576, 1823, 2036
AlwI GGATC 6 cut(s) 223, 587, 773, 812, 1243, 2091
AlwNI CAGNNNCTG 2 cut(s) 129, 1509
Ama87I CYCGRG 1 cut(s) 2582
AoxI GGCC 1 cut(s) 2232
ApeKI GCWGC 4 cut(s) 1509, 1580, 2021, 2146
AseI ATTAAT 1 cut(s) 807
AsiGI ACCGGT 1 cut(s) 2285
Asp700I GAANNNNTTC 4 cut(s) 258, 662, 1055, 2481
AspLEI GCGC 2 cut(s) 829, 2136
AspS9I GGNCC 5 cut(s) 623, 679, 1282, 2233, 2536
AsuC2I CCSGG 1 cut(s) 444
AsuHPI GGTGA 4 cut(s) 514, 1619, 1813, 2102
AsuNHI GCTAGC 1 cut(s) 1180
AvaI CYCGRG 1 cut(s) 2582
AvaII GGWCC 4 cut(s) 623, 679, 1282, 2536
BaeI ACNNNNGTAYC 2 cut(s) 1825, 1858
BanI GGYRCC 1 cut(s) 2133
BanII GRGCYC 3 cut(s) 753, 1272, 2265
BauI CACGAG 1 cut(s) 216
BbsI GAAGAC 4 cut(s) 261, 655, 978, 1341
Bbv12I GWGCWC 3 cut(s) 753, 2265, 2545
BbvI GCAGC 4 cut(s) 1496, 1592, 2008, 2133
BccI CCATC 6 cut(s) 185, 1302, 1487, 2251, 2362, 2586
BceAI ACGGC 3 cut(s) 348, 1335, 1497
BcgI CGANNNNNNTGC 2 cut(s) 984, 1018
BciT130I CCWGG 3 cut(s) 627, 936, 1605
BclI TGATCA 1 cut(s) 2353
BcnI CCSGG 1 cut(s) 444
BcoDI GTCTC 4 cut(s) 204, 1576, 1823, 2036
BfaI CTAG 3 cut(s) 1181, 2138, 2418
BfmI CTRYAG 1 cut(s) 1588
BfoI RGCGCY 1 cut(s) 2137
BfrI CTTAAG 2 cut(s) 1298, 1913
BisI GCNGC 5 cut(s) 719, 1510, 1581, 2022, 2147
BlpI GCTNAGC 1 cut(s) 2514
BlsI GCNGC 5 cut(s) 720, 1511, 1582, 2023, 2148
Bme1390I CCNGG 4 cut(s) 444, 627, 936, 1605
Bme18I GGWCC 4 cut(s) 623, 679, 1282, 2536
BmeT110I CYCGRG 1 cut(s) 2582
BmgT120I GGNCC 5 cut(s) 623, 679, 1282, 2233, 2536
BmiI GGNNCC 2 cut(s) 1269, 2135
BmrFI CCNGG 4 cut(s) 444, 627, 936, 1605
BmrI ACTGGG 3 cut(s) 951, 1123, 1266
BmsI GCATC 6 cut(s) 100, 132, 319, 1383, 2467, 2476
BmtI GCTAGC 1 cut(s) 1184
BmuI ACTGGG 3 cut(s) 951, 1123, 1266
BoxI GACNNNNGTC 1 cut(s) 729
BpiI GAAGAC 4 cut(s) 261, 655, 978, 1341
BplI GAGNNNNNCTC 4 cut(s) 138, 170, 1669, 1701
BpmI CTGGAG 1 cut(s) 1587
Bpu10I CCTNAGC 3 cut(s) 634, 2250, 2394
Bpu1102I GCTNAGC 1 cut(s) 2514
BpuEI CTTGAG 3 cut(s) 1849, 2249, 2420
BpuMI CCSGG 1 cut(s) 444
BsaBI GATNNNNATC 1 cut(s) 2082
BsaHI GRCGYC 2 cut(s) 309, 2134
BsaI GGTCTC 2 cut(s) 204, 1823
BsaJI CCNNGG 3 cut(s) 596, 935, 1231
BsaWI WCCGGW 1 cut(s) 2285
Bsc4I CCNNNNNNNGG 4 cut(s) 941, 1108, 1264, 1830
Bse118I RCCGGY 1 cut(s) 2285
Bse1I ACTGG 6 cut(s) 20, 946, 1118, 1272, 2593, 2608
Bse3DI GCAATG 2 cut(s) 1030, 2394
Bse8I GATNNNNATC 1 cut(s) 2082
BseBI CCWGG 3 cut(s) 627, 936, 1605
BseDI CCNNGG 3 cut(s) 596, 935, 1231
BseGI GGATG 4 cut(s) 147, 1008, 2050, 2565
BseJI GATNNNNATC 1 cut(s) 2082
BseLI CCNNNNNNNGG 4 cut(s) 941, 1108, 1264, 1830
BseMI GCAATG 2 cut(s) 1030, 2394
BseMII CTCAG 3 cut(s) 169, 648, 2241
BseNI ACTGG 6 cut(s) 20, 946, 1118, 1272, 2593, 2608
BseRI GAGGAG 2 cut(s) 1436, 2078
BseXI GCAGC 4 cut(s) 1496, 1592, 2008, 2133
BsgI GTGCAG 1 cut(s) 2310
Bsh1236I CGCG 3 cut(s) 105, 721, 1533
Bsh1285I CGRYCG 1 cut(s) 80
BshFI GGCC 1 cut(s) 2234
BshNI GGYRCC 1 cut(s) 2133
BshTI ACCGGT 1 cut(s) 2285
BsiEI CGRYCG 1 cut(s) 80
BsiHKAI GWGCWC 3 cut(s) 753, 2265, 2545
BsiHKCI CYCGRG 1 cut(s) 2582
BsiSI CCGG 2 cut(s) 443, 2286
BslFI GGGAC 4 cut(s) 269, 459, 717, 952
BslI CCNNNNNNNGG 4 cut(s) 941, 1108, 1264, 1830
BsmAI GTCTC 4 cut(s) 204, 1576, 1823, 2036
BsmFI GGGAC 4 cut(s) 269, 459, 717, 952
BsmI GAATGC 1 cut(s) 701
BsnI GGCC 1 cut(s) 2234
Bso31I GGTCTC 2 cut(s) 204, 1823
BsoBI CYCGRG 1 cut(s) 2582
Bsp1286I GDGCHC 4 cut(s) 753, 1272, 2265, 2545
Bsp1720I GCTNAGC 1 cut(s) 2514
Bsp68I TCGCGA 1 cut(s) 1533
BspACI CCGC 5 cut(s) 38, 103, 719, 1553, 1960
BspANI GGCC 1 cut(s) 2234
BspCNI CTCAG 3 cut(s) 168, 647, 2242
BspFNI CGCG 3 cut(s) 105, 721, 1533
BspHI TCATGA 2 cut(s) 811, 1633
BspLI GGNNCC 2 cut(s) 1269, 2135
BspMAI CTGCAG 1 cut(s) 1592
BspOI GCTAGC 1 cut(s) 1184
BspPI GGATC 6 cut(s) 223, 587, 773, 812, 1243, 2091
BspQI GCTCTTC 1 cut(s) 112
BspT107I GGYRCC 1 cut(s) 2133
BspTI CTTAAG 2 cut(s) 1298, 1913
BspTNI GGTCTC 2 cut(s) 204, 1823
BsrDI GCAATG 2 cut(s) 1030, 2394
BsrFI RCCGGY 1 cut(s) 2285
BsrI ACTGG 6 cut(s) 20, 946, 1118, 1272, 2593, 2608
BssAI RCCGGY 1 cut(s) 2285
BssECI CCNNGG 3 cut(s) 596, 935, 1231
BssNI GRCGYC 2 cut(s) 309, 2134
BssSI CACGAG 1 cut(s) 216
BssT1I CCWWGG 2 cut(s) 596, 1231
Bst2BI CACGAG 1 cut(s) 216
Bst2UI CCWGG 3 cut(s) 627, 936, 1605
Bst4CI ACNGT 9 cut(s) 180, 207, 349, 502, 687, 1570, 2349, 2531, 2540
Bst6I CTCTTC 3 cut(s) 112, 1006, 1492
BstACI GRCGYC 2 cut(s) 309, 2134
BstAFI CTTAAG 2 cut(s) 1298, 1913
BstC8I GCNNGC 4 cut(s) 147, 361, 1148, 1182
BstDEI CTNAG 7 cut(s) 155, 634, 1844, 1906, 2250, 2394, 2514
BstF5I GGATG 4 cut(s) 147, 1008, 2050, 2565
BstFNI CGCG 3 cut(s) 105, 721, 1533
BstH2I RGCGCY 1 cut(s) 2137
BstHHI GCGC 2 cut(s) 829, 2136
BstMAI GTCTC 4 cut(s) 204, 1576, 1823, 2036
BstMCI CGRYCG 1 cut(s) 80
BstMWI GCNNNNNNNGC 8 cut(s) 11, 81, 119, 1163, 2240, 2260, 2404, 2464
BstNI CCWGG 3 cut(s) 627, 936, 1605
BstNSI RCATGY 3 cut(s) 766, 1327, 2497
BstPAI GACNNNNGTC 1 cut(s) 729
BstSCI CCNGG 4 cut(s) 442, 625, 934, 1603
BstSFI CTRYAG 1 cut(s) 1588
BstUI CGCG 3 cut(s) 105, 721, 1533
BstV1I GCAGC 4 cut(s) 1496, 1592, 2008, 2133
BstV2I GAAGAC 4 cut(s) 261, 655, 978, 1341
BstX2I RGATCY 3 cut(s) 228, 592, 817
BstXI CCANNNNNNTGG 1 cut(s) 1209
BstYI RGATCY 3 cut(s) 228, 592, 817
BsuRI GGCC 1 cut(s) 2234
BtgZI GCGATG 2 cut(s) 120, 2494
BtsCI GGATG 4 cut(s) 147, 1008, 2050, 2565
BtsIMutI CAGTG 4 cut(s) 163, 1566, 2536, 2601
BtuMI TCGCGA 1 cut(s) 1533
Cac8I GCNNGC 4 cut(s) 147, 361, 1148, 1182
CaiI CAGNNNCTG 2 cut(s) 129, 1509
CciI TCATGA 2 cut(s) 811, 1633
CfoI GCGC 2 cut(s) 829, 2136
Cfr10I RCCGGY 1 cut(s) 2285
Cfr13I GGNCC 5 cut(s) 623, 679, 1282, 2233, 2536
CseI GACGC 1 cut(s) 972
CspAI ACCGGT 1 cut(s) 2285
DdeI CTNAG 7 cut(s) 155, 634, 1844, 1906, 2250, 2394, 2514
DinI GGCGCC 1 cut(s) 2135
DraI TTTAAA 2 cut(s) 1753, 2193
Eam1104I CTCTTC 3 cut(s) 112, 1006, 1492
EarI CTCTTC 3 cut(s) 112, 1006, 1492
EciI GGCGGA 1 cut(s) 53
Ecl136II GAGCTC 2 cut(s) 751, 2263
Eco130I CCWWGG 2 cut(s) 596, 1231
Eco24I GRGCYC 3 cut(s) 753, 1272, 2265
Eco31I GGTCTC 2 cut(s) 204, 1823
Eco47I GGWCC 4 cut(s) 623, 679, 1282, 2536
Eco53kI GAGCTC 2 cut(s) 751, 2263
Eco57I CTGAAG 4 cut(s) 996, 1353, 1401, 2192
Eco88I CYCGRG 1 cut(s) 2582
EcoICRI GAGCTC 2 cut(s) 751, 2263
EcoO109I RGGNCCY 3 cut(s) 623, 1282, 2233
EcoRII CCWGG 3 cut(s) 625, 934, 1603
EcoT14I CCWWGG 2 cut(s) 596, 1231
EcoT38I GRGCYC 3 cut(s) 753, 1272, 2265
EgeI GGCGCC 1 cut(s) 2135
EheI GGCGCC 1 cut(s) 2135
ErhI CCWWGG 2 cut(s) 596, 1231
FalI AAGNNNNNCTT 4 cut(s) 246, 278, 647, 679
FaqI GGGAC 4 cut(s) 269, 459, 717, 952
FauI CCCGC 1 cut(s) 110
FbaI TGATCA 1 cut(s) 2353
Fnu4HI GCNGC 5 cut(s) 719, 1510, 1581, 2022, 2147
FokI GGATG 4 cut(s) 154, 995, 2057, 2552
FriOI GRGCYC 3 cut(s) 753, 1272, 2265
Fsp4HI GCNGC 5 cut(s) 719, 1510, 1581, 2022, 2147
FspBI CTAG 3 cut(s) 1181, 2138, 2418
GlaI GCGC 2 cut(s) 828, 2135
GluI GCNGC 5 cut(s) 719, 1510, 1581, 2022, 2147
GsuI CTGGAG 1 cut(s) 1587
HaeII RGCGCY 1 cut(s) 2137
HaeIII GGCC 1 cut(s) 2234
HapII CCGG 2 cut(s) 443, 2286
HgaI GACGC 1 cut(s) 972
HhaI GCGC 2 cut(s) 829, 2136
Hin1I GRCGYC 2 cut(s) 309, 2134
Hin6I GCGC 2 cut(s) 827, 2134
HinP1I GCGC 2 cut(s) 827, 2134
HindIII AAGCTT 5 cut(s) 1044, 1886, 2241, 2396, 2516
HinfI GANTC 8 cut(s) 505, 800, 1051, 1500, 1721, 1805, 1893, 2562
HpaII CCGG 2 cut(s) 443, 2286
HphI GGTGA 4 cut(s) 514, 1619, 1813, 2102
Hpy166II GTNNAC 2 cut(s) 740, 1517
Hpy188I TCNGA 8 cut(s) 233, 318, 376, 606, 976, 1443, 1505, 1726
Hpy8I GTNNAC 2 cut(s) 740, 1517
Hpy99I CGWCG 2 cut(s) 311, 2477
HpyAV CCTTC 8 cut(s) 790, 841, 1079, 1136, 1295, 1376, 2246, 2608
HpyCH4III ACNGT 9 cut(s) 180, 207, 349, 502, 687, 1570, 2349, 2531, 2540
HpyCH4IV ACGT 3 cut(s) 258, 309, 2315
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 81, 119, 1163, 2240, 2260, 2404, 2464
HpyF3I CTNAG 7 cut(s) 155, 634, 1844, 1906, 2250, 2394, 2514
HpySE526I ACGT 3 cut(s) 258, 309, 2315
Hsp92I GRCGYC 2 cut(s) 309, 2134
HspAI GCGC 2 cut(s) 827, 2134
KasI GGCGCC 1 cut(s) 2133
Ksp22I TGATCA 1 cut(s) 2353
LguI GCTCTTC 1 cut(s) 112
LmnI GCTCC 7 cut(s) 885, 1267, 1606, 1962, 2260, 2455, 2586
Lsp1109I GCAGC 4 cut(s) 1496, 1592, 2008, 2133
LweI GCATC 6 cut(s) 100, 132, 319, 1383, 2467, 2476
MaeI CTAG 3 cut(s) 1181, 2138, 2418
MaeII ACGT 3 cut(s) 258, 309, 2315
MaeIII GTNAC 9 cut(s) 158, 502, 722, 968, 1218, 1274, 1927, 1996, 2008
MfeI CAATTG 1 cut(s) 830
MflI RGATCY 3 cut(s) 228, 592, 817
MhlI GDGCHC 4 cut(s) 753, 1272, 2265, 2545
Mly113I GGCGCC 1 cut(s) 2134
MlyI GAGTC 3 cut(s) 499, 1509, 2571
MmeI TCCRAC 2 cut(s) 706, 1121
MroXI GAANNNNTTC 4 cut(s) 258, 662, 1055, 2481
MslI CAYNNNNRTG 2 cut(s) 1925, 2286
MspA1I CMGCKG 1 cut(s) 1166
MspCI CTTAAG 2 cut(s) 1298, 1913
MspI CCGG 2 cut(s) 443, 2286
MspR9I CCNGG 4 cut(s) 444, 627, 936, 1605
MunI CAATTG 1 cut(s) 830
Mva1269I GAATGC 1 cut(s) 701
MvaI CCWGG 3 cut(s) 627, 936, 1605
MvnI CGCG 3 cut(s) 105, 721, 1533
MwoI GCNNNNNNNGC 8 cut(s) 11, 81, 119, 1163, 2240, 2260, 2404, 2464
NarI GGCGCC 1 cut(s) 2134
NciI CCSGG 1 cut(s) 444
NheI GCTAGC 1 cut(s) 1180
NlaIV GGNNCC 2 cut(s) 1269, 2135
NmuCI GTSAC 6 cut(s) 158, 502, 722, 1927, 1996, 2008
NruI TCGCGA 1 cut(s) 1533
NspI RCATGY 3 cut(s) 766, 1327, 2497
OliI CACNNNNGTG 2 cut(s) 1925, 2286
PagI TCATGA 2 cut(s) 811, 1633
PciI ACATGT 1 cut(s) 762
PciSI GCTCTTC 1 cut(s) 112
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PctI GAATGC 1 cut(s) 701
PdmI GAANNNNTTC 4 cut(s) 258, 662, 1055, 2481
PfeI GAWTC 5 cut(s) 800, 1051, 1721, 1805, 1893
PflMI CCANNNNNTGG 2 cut(s) 1108, 1264
PfoI TCCNGGA 1 cut(s) 442
PinAI ACCGGT 1 cut(s) 2285
PkrI GCNGC 5 cut(s) 720, 1511, 1582, 2023, 2148
Ple19I CGATCG 1 cut(s) 80
PleI GAGTC 3 cut(s) 499, 1508, 2570
PluTI GGCGCC 1 cut(s) 2137
PpsI GAGTC 3 cut(s) 499, 1508, 2570
PpuMI RGGWCCY 2 cut(s) 623, 1282
PscI ACATGT 1 cut(s) 762
PshAI GACNNNNGTC 1 cut(s) 729
PshBI ATTAAT 1 cut(s) 807
PsiI TTATAA 1 cut(s) 1953
Psp124BI GAGCTC 2 cut(s) 753, 2265
Psp1406I AACGTT 1 cut(s) 2315
Psp5II RGGWCCY 2 cut(s) 623, 1282
Psp6I CCWGG 3 cut(s) 625, 934, 1603
PspGI CCWGG 3 cut(s) 625, 934, 1603
PspN4I GGNNCC 2 cut(s) 1269, 2135
PspPI GGNCC 5 cut(s) 623, 679, 1282, 2233, 2536
PspPPI RGGWCCY 2 cut(s) 623, 1282
PstI CTGCAG 1 cut(s) 1592
PstNI CAGNNNCTG 2 cut(s) 129, 1509
PsuI RGATCY 3 cut(s) 228, 592, 817
PvuI CGATCG 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 1166
RruI TCGCGA 1 cut(s) 1533
RseI CAYNNNNRTG 2 cut(s) 1925, 2286
SacI GAGCTC 2 cut(s) 753, 2265
SapI GCTCTTC 1 cut(s) 112
SatI GCNGC 5 cut(s) 719, 1510, 1581, 2022, 2147
Sau96I GGNCC 5 cut(s) 623, 679, 1282, 2233, 2536
SchI GAGTC 3 cut(s) 499, 1509, 2571
ScrFI CCNGG 4 cut(s) 444, 627, 936, 1605
SduI GDGCHC 4 cut(s) 753, 1272, 2265, 2545
SfaNI GCATC 6 cut(s) 100, 132, 319, 1383, 2467, 2476
SfcI CTRYAG 1 cut(s) 1588
SfoI GGCGCC 1 cut(s) 2135
SgrAI CRCCGGYG 1 cut(s) 2285
SinI GGWCC 4 cut(s) 623, 679, 1282, 2536
SmiMI CAYNNNNRTG 2 cut(s) 1925, 2286
SmlI CTYRAG 5 cut(s) 1298, 1828, 1913, 2228, 2399
SmoI CTYRAG 5 cut(s) 1298, 1828, 1913, 2228, 2399
SsiI CCGC 5 cut(s) 38, 103, 719, 1553, 1960
SspDI GGCGCC 1 cut(s) 2133
SspI AATATT 2 cut(s) 391, 1263
SspMI CTAG 3 cut(s) 1181, 2138, 2418
SstI GAGCTC 2 cut(s) 753, 2265
StyD4I CCNGG 4 cut(s) 442, 625, 934, 1603
StyI CCWWGG 2 cut(s) 596, 1231
TaaI ACNGT 9 cut(s) 180, 207, 349, 502, 687, 1570, 2349, 2531, 2540
TaiI ACGT 3 cut(s) 261, 312, 2318
TaqI TCGA 9 cut(s) 60, 80, 1491, 1618, 2036, 2265, 2376, 2472, 2560
TatI WGTACW 3 cut(s) 1314, 1516, 1682
TauI GCSGC 1 cut(s) 721
TfiI GAWTC 5 cut(s) 800, 1051, 1721, 1805, 1893
TscAI CASTG 4 cut(s) 163, 1573, 2536, 2608
TseFI GTSAC 6 cut(s) 158, 502, 722, 1927, 1996, 2008
TseI GCWGC 4 cut(s) 1509, 1580, 2021, 2146
Tsp45I GTSAC 6 cut(s) 158, 502, 722, 1927, 1996, 2008
TspRI CASTG 4 cut(s) 163, 1573, 2536, 2608
Van91I CCANNNNNTGG 2 cut(s) 1108, 1264
Vha464I CTTAAG 2 cut(s) 1298, 1913
VpaK11BI GGWCC 4 cut(s) 623, 679, 1282, 2536
VspI ATTAAT 1 cut(s) 807
XbaI TCTAGA 1 cut(s) 2417
XceI RCATGY 3 cut(s) 766, 1327, 2497
XcmI CCANNNNNNNNNTGG 1 cut(s) 2600
XmnI GAANNNNTTC 4 cut(s) 258, 662, 1055, 2481
XspI CTAG 3 cut(s) 1181, 2138, 2418
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.