Rorug02G0013700

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000865
Physical Location & Seq
Reverse (-)
1165239 .. 1168702
3464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0013700.1

Sequence Viewer

Length: 879 bp
ATGGGCAATGTAACGTCTGGTGTGGCTGCAAAGTTTGCGTTCTTTCCACCAGACCCGCCGACCTATGATGTGTCGAAGGACGAAGGAGGGAAGCTTGTGTTCTCTGGAGTCTCGGGAGATAAGAACATGGATGTGCATTTGGTTGAGACCAAAGGAGGAAACAAAGTCGTTGCCACGTTTTGGAAGCACCCTTTTGCCAGGTTCACCATTCTTTACTCGCATGGCAATGCTGCTGATTTGGGTCAGATGCATGAGCTCTTCATTGAGCTCAGGGCTCACCTCAGGGTTAATATCATGAGCTATGATTATTCAGGATATGGAGCATCCAGCGGTAAGCCATCTGAGTTCAACACCTATCATGACATAGAAGCTGTGTACAATTGCTTAAAGAGCGAATATGGAGTAAAGCAAGAAGATTTGATACTTTACGGCCAATCTGTTGGAAGTGGACCAACACTGCACATGGCTTCTCGGTTAAAGAGGTTGAGAGCTGTTGTTCTTCATAGTGCAATCCTTTCAGGCATACGAGTCTTGTATAATATCAAAGTGACATTTTGGTTCGACATTTTTAAAAATATAGACAAAATACGGCATGTCACCTGTCCGGTTTTAGTTATACATGGAACAGAAGATGACATTGTTGATTTGTCCCATGGGAAGCGTTTATGGGAACTTGCCAAGGAAAAATATGACCCCTTATGGGTCAAGGGCGGAGGCCATTGCAACCTAGAGACGTATCCAGAGTACATTAAGCACTTACGCAAGTTCATAAATGCCATGGAGAAACTCTCACTCTCGAATCAAACGAAGAAAGAACTCACTTCTACCCCAAGTATTGAATTAAAACACAACAAGTGCTTGAGATTTGGAAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

292

Amino Acids

32.94

Weight (kDa)

9.04

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 68 - 173 4.1e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 70 - 170 2.7e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 180
AciI CCGC 3 cut(s) 56, 330, 711
AcoI YGGCCR 1 cut(s) 430
AfaI GTAC 2 cut(s) 377, 746
AfiI CCNNNNNNNGG 2 cut(s) 180, 700
AgsI TTSAA 2 cut(s) 349, 839
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 6 cut(s) 94, 256, 268, 300, 371, 491
AluI AGCT 6 cut(s) 94, 256, 268, 300, 371, 491
Alw21I GWGCWC 2 cut(s) 258, 270
Alw26I GTCTC 3 cut(s) 115, 140, 725
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 2 cut(s) 430, 715
ApeKI GCWGC 2 cut(s) 26, 230
AspS9I GGNCC 1 cut(s) 449
AsuHPI GGTGA 3 cut(s) 196, 269, 589
AvaI CYCGRG 1 cut(s) 112
AvaII GGWCC 1 cut(s) 449
AxyI CCTNAGG 1 cut(s) 281
BanII GRGCYC 3 cut(s) 258, 270, 277
BarI GAAGNNNNNNTAC 2 cut(s) 405, 437
Bbv12I GWGCWC 2 cut(s) 258, 270
BbvI GCAGC 2 cut(s) 13, 217
BccI CCATC 1 cut(s) 346
BceAI ACGGC 2 cut(s) 445, 605
BciT130I CCWGG 1 cut(s) 199
BciVI GTATCC 1 cut(s) 747
BcoDI GTCTC 3 cut(s) 115, 140, 725
BfaI CTAG 1 cut(s) 728
BfuI GTATCC 1 cut(s) 747
BisI GCNGC 2 cut(s) 27, 231
BlsI GCNGC 2 cut(s) 28, 232
Bme1390I CCNGG 1 cut(s) 199
Bme18I GGWCC 1 cut(s) 449
BmeT110I CYCGRG 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 449
BmrFI CCNGG 1 cut(s) 199
BmsI GCATC 2 cut(s) 237, 332
BplI GAGNNNNNCTC 2 cut(s) 773, 805
BpmI CTGGAG 1 cut(s) 126
Bpu10I CCTNAGC 1 cut(s) 269
BsaI GGTCTC 1 cut(s) 140
BsaJI CCNNGG 3 cut(s) 652, 678, 777
BsaWI WCCGGW 1 cut(s) 604
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 700
Bse21I CCTNAGG 1 cut(s) 281
Bse3DI GCAATG 3 cut(s) 13, 232, 718
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 3 cut(s) 652, 678, 777
BseGI GGATG 2 cut(s) 136, 323
BseLI CCNNNNNNNGG 2 cut(s) 180, 700
BseMI GCAATG 3 cut(s) 13, 232, 718
BseMII CTCAG 3 cut(s) 283, 295, 333
BseXI GCAGC 2 cut(s) 13, 217
BsgI GTGCAG 1 cut(s) 443
BshFI GGCC 2 cut(s) 432, 717
BsiHKAI GWGCWC 2 cut(s) 258, 270
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 1 cut(s) 605
BslFI GGGAC 1 cut(s) 634
BslI CCNNNNNNNGG 2 cut(s) 180, 700
BsmAI GTCTC 3 cut(s) 115, 140, 725
BsmBI CGTCTC 1 cut(s) 725
BsmFI GGGAC 1 cut(s) 634
BsnI GGCC 2 cut(s) 432, 717
Bso31I GGTCTC 1 cut(s) 140
BsoBI CYCGRG 1 cut(s) 112
Bsp1286I GDGCHC 3 cut(s) 258, 270, 277
Bsp1407I TGTACA 1 cut(s) 375
Bsp19I CCATGG 2 cut(s) 652, 777
BspACI CCGC 3 cut(s) 56, 330, 711
BspANI GGCC 2 cut(s) 432, 717
BspCNI CTCAG 3 cut(s) 282, 294, 334
BspHI TCATGA 2 cut(s) 294, 358
BspQI GCTCTTC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 140
BsrDI GCAATG 3 cut(s) 13, 232, 718
BsrGI TGTACA 1 cut(s) 375
BssECI CCNNGG 3 cut(s) 652, 678, 777
BssT1I CCWWGG 3 cut(s) 652, 678, 777
Bst2UI CCWGG 1 cut(s) 199
Bst6I CTCTTC 1 cut(s) 263
BstAPI GCANNNNNTGC 1 cut(s) 35
BstAUI TGTACA 1 cut(s) 375
BstDEI CTNAG 3 cut(s) 269, 281, 342
BstDSI CCRYGG 2 cut(s) 652, 777
BstF5I GGATG 2 cut(s) 136, 323
BstMAI GTCTC 3 cut(s) 115, 140, 725
BstMWI GCNNNNNNNGC 2 cut(s) 35, 390
BstNI CCWGG 1 cut(s) 199
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 1 cut(s) 197
BstV1I GCAGC 2 cut(s) 13, 217
BstXI CCANNNNNNTGG 1 cut(s) 440
Bsu36I CCTNAGG 1 cut(s) 281
BsuI GTATCC 1 cut(s) 747
BsuRI GGCC 2 cut(s) 432, 717
BtgI CCRYGG 2 cut(s) 652, 777
BtsCI GGATG 2 cut(s) 136, 323
BtsI GCAGTG 1 cut(s) 455
BtsIMutI CAGTG 1 cut(s) 455
CciI TCATGA 2 cut(s) 294, 358
Cfr13I GGNCC 1 cut(s) 449
Csp6I GTAC 2 cut(s) 376, 745
CviQI GTAC 2 cut(s) 376, 745
DdeI CTNAG 3 cut(s) 269, 281, 342
DraI TTTAAA 1 cut(s) 571
EaeI YGGCCR 1 cut(s) 430
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
EciI GGCGGA 1 cut(s) 726
Ecl136II GAGCTC 2 cut(s) 256, 268
Eco130I CCWWGG 3 cut(s) 652, 678, 777
Eco24I GRGCYC 3 cut(s) 258, 270, 277
Eco31I GGTCTC 1 cut(s) 140
Eco47I GGWCC 1 cut(s) 449
Eco53kI GAGCTC 2 cut(s) 256, 268
Eco81I CCTNAGG 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 112
EcoICRI GAGCTC 2 cut(s) 256, 268
EcoRII CCWGG 1 cut(s) 197
EcoT14I CCWWGG 3 cut(s) 652, 678, 777
EcoT22I ATGCAT 1 cut(s) 252
EcoT38I GRGCYC 3 cut(s) 258, 270, 277
ErhI CCWWGG 3 cut(s) 652, 678, 777
Esp3I CGTCTC 1 cut(s) 725
FaqI GGGAC 1 cut(s) 634
FauI CCCGC 1 cut(s) 63
Fnu4HI GCNGC 2 cut(s) 27, 231
FokI GGATG 2 cut(s) 143, 310
FriOI GRGCYC 3 cut(s) 258, 270, 277
Fsp4HI GCNGC 2 cut(s) 27, 231
FspBI CTAG 1 cut(s) 728
GluI GCNGC 2 cut(s) 27, 231
GsuI CTGGAG 1 cut(s) 126
HaeIII GGCC 2 cut(s) 432, 717
HapII CCGG 1 cut(s) 605
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 3 cut(s) 108, 528, 799
HpaII CCGG 1 cut(s) 605
HphI GGTGA 3 cut(s) 196, 269, 589
Hpy166II GTNNAC 3 cut(s) 204, 376, 449
Hpy188I TCNGA 2 cut(s) 246, 343
Hpy188III TCNNGA 7 cut(s) 105, 114, 295, 312, 359, 740, 796
Hpy8I GTNNAC 3 cut(s) 204, 376, 449
HpyAV CCTTC 2 cut(s) 70, 77
HpyCH4IV ACGT 3 cut(s) 14, 176, 734
HpyCH4V TGCA 6 cut(s) 29, 136, 250, 460, 509, 723
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 390
HpyF3I CTNAG 3 cut(s) 269, 281, 342
HpySE526I ACGT 3 cut(s) 14, 176, 734
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 2 cut(s) 13, 217
LweI GCATC 2 cut(s) 237, 332
MaeI CTAG 1 cut(s) 728
MaeII ACGT 3 cut(s) 14, 176, 734
MaeIII GTNAC 3 cut(s) 10, 547, 595
MboII GAAGA 5 cut(s) 250, 425, 491, 641, 820
MfeI CAATTG 1 cut(s) 379
MhlI GDGCHC 3 cut(s) 258, 270, 277
MluCI AATT 2 cut(s) 379, 839
MlyI GAGTC 2 cut(s) 117, 537
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 5 cut(s) 80, 149, 290, 474, 707
Mph1103I ATGCAT 1 cut(s) 252
MseI TTAA 6 cut(s) 288, 386, 476, 570, 750, 842
MslI CAYNNNNRTG 2 cut(s) 131, 225
MspA1I CMGCKG 1 cut(s) 330
MspI CCGG 1 cut(s) 605
MspR9I CCNGG 1 cut(s) 199
MunI CAATTG 1 cut(s) 379
MvaI CCWGG 1 cut(s) 199
MwoI GCNNNNNNNGC 2 cut(s) 35, 390
NcoI CCATGG 2 cut(s) 652, 777
NmuCI GTSAC 2 cut(s) 547, 595
NsiI ATGCAT 1 cut(s) 252
NspI RCATGY 1 cut(s) 596
PagI TCATGA 2 cut(s) 294, 358
PciSI GCTCTTC 1 cut(s) 263
PcsI WCGNNNNNNNCGW 1 cut(s) 803
PfeI GAWTC 1 cut(s) 799
PflMI CCANNNNNTGG 1 cut(s) 180
PkrI GCNGC 2 cut(s) 28, 232
PleI GAGTC 2 cut(s) 116, 536
PpsI GAGTC 2 cut(s) 116, 536
Psp124BI GAGCTC 2 cut(s) 258, 270
Psp6I CCWGG 1 cut(s) 197
PspGI CCWGG 1 cut(s) 197
PspPI GGNCC 1 cut(s) 449
RsaI GTAC 2 cut(s) 377, 746
RsaNI GTAC 2 cut(s) 376, 745
RseI CAYNNNNRTG 2 cut(s) 131, 225
SacI GAGCTC 2 cut(s) 258, 270
SapI GCTCTTC 1 cut(s) 263
SaqAI TTAA 6 cut(s) 288, 386, 476, 570, 750, 842
SatI GCNGC 2 cut(s) 27, 231
Sau96I GGNCC 1 cut(s) 449
SchI GAGTC 2 cut(s) 117, 537
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 3 cut(s) 258, 270, 277
SfaNI GCATC 2 cut(s) 237, 332
SinI GGWCC 1 cut(s) 449
SmiMI CAYNNNNRTG 2 cut(s) 131, 225
SmlI CTYRAG 1 cut(s) 859
SmoI CTYRAG 1 cut(s) 859
Sse9I AATT 2 cut(s) 379, 839
SsiI CCGC 3 cut(s) 56, 330, 711
SspMI CTAG 1 cut(s) 728
SstI GAGCTC 2 cut(s) 258, 270
StyD4I CCNGG 1 cut(s) 197
StyI CCWWGG 3 cut(s) 652, 678, 777
TaiI ACGT 3 cut(s) 17, 179, 737
TaqI TCGA 3 cut(s) 74, 561, 797
TasI AATT 2 cut(s) 379, 839
TatI WGTACW 2 cut(s) 375, 744
TfiI GAWTC 1 cut(s) 799
Tru1I TTAA 6 cut(s) 288, 386, 476, 570, 750, 842
Tru9I TTAA 6 cut(s) 288, 386, 476, 570, 750, 842
TscAI CASTG 1 cut(s) 462
TseFI GTSAC 2 cut(s) 547, 595
TseI GCWGC 2 cut(s) 26, 230
Tsp45I GTSAC 2 cut(s) 547, 595
TspDTI ATGAA 3 cut(s) 250, 491, 757
TspRI CASTG 1 cut(s) 462
Van91I CCANNNNNTGG 1 cut(s) 180
VpaK11BI GGWCC 1 cut(s) 449
XceI RCATGY 1 cut(s) 596
XspI CTAG 1 cut(s) 728
Zsp2I ATGCAT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.