RLG00000016184

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000865
Physical Location & Seq
Forward (+)
4560531 .. 4563068
2538 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016184

Sequence Viewer

Length: 2538 bp
ATGGATGCCTATCTAATCGACTTCGAAGGTGGCATTTATGAAAACAGACACCAAATCCAAGAGATAGCTACTGATATCGTGGAACCTCTTGACAAATTGATGCATAAGCTGGTTTCACACGATCATGGTGTTGACAAGTTGATGCATAAGCAGGTTTTGAAATTGATGAAACAAGTCGCATTTTGGGTCACAAGTCTCAATTTGTCCTCAAAAATTGATAGAATTGTTGTGAAGAACACTGATTTTGTCAGAAAACTAGCTTCACAGCCATACGACTCGCAATCGAGACTAGGCGGCTGTTCACCACAGGATAGTGAACTTGCAAACTATAACGAAGTACAGGGCTTTGAAGCGCTTGCACAAAAACTCTTCGAAAATTTGATAGATGAAGAGCCACAGCTTTCAACAATTTGTGTTGTCGGTCCTGGTGGATCTGGAAAATCCACCCTGGTAAATTTGGTTTTCAAGTGGAAGGTTGTAGAGTTGTTTGAGTGCAGGACTTGGGTTCGTGTGACGCGTCGGCCACTTCCACTCAAGGAACTCCTGCAACACATGCTGACTGATTTTGGGTATAAAAATTCAGAAGAAATCGATGATCCACGAGCAAAATTGAGACTTTTTCTGCAGCAAAGGAGGTATCTGGTTGTCTTAGACGATGTTTGGAGTGAACAAGATTTTTATTGCATTGTAAAAGCGTTACCAAATGGTGCTCCCGGAAGTAAGATGATAATAACTAGTCGTAATTCTAGTGTCGTCTCTTTTCTCTCCCCCAAGTATATCCATGATTTGAGTAGTGGGTTGTCACCTGAAGCGGCCAAGAATCTATTCACCAGGAAGGTTCTCCGGAATCATCTAGGAAATTTGCAACCCGAGGTGGAGGAGTGCACTGATAAAATTCTTCAATGGTGCGGGAACCAGCCCTTTGCAATTTCAGCGGTCGGTAGTTTGCTAGCAGAGAAGCCACAAACTCGGGCAGAGTGGGAGCATATTTTTAACAATCTTGGATCTAAAACTGGACCGGGCTCTATTCTTGAAATTGTTAGCAAAATTTTGCAGCCCAGTTTTATGGATCTTCCGAACCATCTTAAATCCTGTTTTCTGTACTTGAGCATTTTTCCTCTCAACTATGCCATCCAGCCTGAGAGGTTAATTCACTTATGGGTTGCGGAAGGACTTGCGGAACGTTATGCCTCCTCTAGTAAGATGCCGAAGGAGTATGTTGCACAAAGTTATTTGAATGAACTTATCTCCAGAAATCTGGTTCTTGCCACTGAAAGAGAAATCAATGGACAACTACAGAGTTGTTGTGCTGTTTTGCACCACGTCCGTGATTACATCCTTTCAAAAGAGGAGAATTTCGTTACTGTTTTGGAGTCTGACCCGGACCGGAGGACTAGGCCTGCATTTGAGAAAATTCGACGCCTTTCAGTGTTAAAAGCTGATTGTGTCAGTCTGCTGTACTCTTTCAACTTGAATCATTTGCATACCTTAGTGGTCTTTGGTAGTTGGTCTTATTCTGAGCTTGAATGGGTACTTGAAAACTGTAAATATTTGAGGGTTTTAGATTTGCAAGGAGTAACTAATTTGAAGGAGTTTCCCGAAAACTTTTCCATTAAGGGTCTCATTCTCTTGAAGTACTTGAGTCTGCGGGGATCAAGGAATACAACAGTTCCAAAGTCCATAAGAAAGTATCAATGCTTGGAGACCTTAGTTCTTGAAGACACCATGGTGATCAAATTGCCAGAAAAAGAGATGCGTGTACTCAAGAATCTGCGCCATTTTTCAGTCTCGTCCATTCATACTGGCGGAGTGATGTTATCGACTGGCAATATTAAAGCTTTATCTTCCATACACGGGTTGTCACTGATTAAGGTGAAAAACAACAGAGCAATCATAAAAGCCTTGGGAGAGTTGAAAAATGTTAGGAATTTAGGACTGGTAGATCTTGAAACACAAGATGGAAAAGAACTATGTACTGCCCTTGAGAAAATGAAGCATCTTTCGACACTTGATATAAGAGCAGAACGCCTGGAGTTCCTTGATTTGGATCATATGCGATCCCCTCCTCATTTTCTCCAGGGTCTACACTTGGAAGGACGCCTAGAAGGGCTACCTAAGTGGATTCTCCAGCTTAGTAGTCTAGAGAAGATTGGTTTACATGGTTCAGAGCTGAGGGCTAACGTTAACCCACTTGAGCTCCTTGAAGCTCTGCCTTATCTGCTTGAGCTTGACTTGGTGGCCTATTACAAAGGGGAAAAGTTGATATTCACAGCTGGTACATTCAAGCGTCTAATGATATTAAAAATGGAACAATTTACTGAGCTGAATTCCATTTCATTTGACGACGGAGCAATGCCGCGGCTTAGGGAGCTAACCATCTCTAGATGTCCGAATCTGAGTTATCCTCCAAATGGCATTCGTGGTCTTAGTTCACTCTACAAGCTGGATCTGGATTACATGCCCCATAATCTTATTCAATTGTCCGCACCGTCAGATGGTTCAGCATATTCCTGTCTCCAATTTTACAACTGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

846

Amino Acids

96.21

Weight (kDa)

7.57

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 118 - 285 8.7e-27 NB-ARC domain
WHD_DRP PF23559 371 - 442 2.7e-12 Disease resistance protein Winged helix domain
LRR_14 PF23598 510 - 796 3.3e-36 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 142
AccI GTMKAC 1 cut(s) 2083
AccII CGCG 2 cut(s) 517, 2359
AccIII TCCGGA 1 cut(s) 843
AclI AACGTT 2 cut(s) 1183, 2181
AclWI GGATC 8 cut(s) 439, 590, 1012, 1077, 1660, 2052, 2055, 2454
AcoI YGGCCR 2 cut(s) 521, 813
AcuI CTGAAG 1 cut(s) 828
AcyI GRCGYC 2 cut(s) 1420, 2098
AfaI GTAC 8 cut(s) 339, 1103, 1460, 1533, 1637, 1761, 1975, 2278
AfeI AGCGCT 1 cut(s) 354
AfiI CCNNNNNNNGG 4 cut(s) 1854, 2044, 2411, 2495
AflIII ACRYGT 1 cut(s) 515
AhlI ACTAGT 1 cut(s) 734
AjiI CACGTC 1 cut(s) 1324
AjnI CCWGG 5 cut(s) 424, 447, 830, 2028, 2076
AjuI GAANNNNNNNTTGG 2 cut(s) 809, 841
AleI CACNNNNGTG 2 cut(s) 1326, 1727
Alw21I GWGCWC 3 cut(s) 712, 887, 2199
Alw26I GTCTC 8 cut(s) 200, 280, 607, 760, 1625, 1697, 1792, 2519
Alw44I GTGCAC 1 cut(s) 883
AlwI GGATC 8 cut(s) 439, 590, 1012, 1077, 1660, 2052, 2055, 2454
Ama87I CYCGRG 2 cut(s) 869, 969
Aor13HI TCCGGA 1 cut(s) 843
Aor51HI AGCGCT 1 cut(s) 354
AoxI GGCC 4 cut(s) 521, 813, 1397, 2238
ApaLI GTGCAC 1 cut(s) 883
ApeKI GCWGC 2 cut(s) 625, 1054
Asp700I GAANNNNTTC 1 cut(s) 824
AspLEI GCGC 2 cut(s) 355, 1776
AspS9I GGNCC 3 cut(s) 422, 1016, 1384
AsuC2I CCSGG 3 cut(s) 714, 1020, 1382
AsuHPI GGTGA 5 cut(s) 294, 795, 820, 1741, 1885
AsuII TTCGAA 2 cut(s) 24, 372
AsuNHI GCTAGC 1 cut(s) 949
AvaI CYCGRG 2 cut(s) 869, 969
AvaII GGWCC 3 cut(s) 422, 1016, 1384
BaeGI GKGCMC 1 cut(s) 887
BanII GRGCYC 2 cut(s) 1025, 2199
BauI CACGAG 1 cut(s) 600
BbsI GAAGAC 1 cut(s) 1725
Bbv12I GWGCWC 3 cut(s) 712, 887, 2199
BbvCI CCTCAGC 1 cut(s) 2171
BbvI GCAGC 2 cut(s) 637, 1066
BccI CCATC 5 cut(s) 1089, 1139, 1952, 2384, 2489
BciT130I CCWGG 5 cut(s) 426, 449, 832, 2030, 2078
BclI TGATCA 1 cut(s) 1731
BcnI CCSGG 3 cut(s) 714, 1020, 1382
BcoDI GTCTC 8 cut(s) 200, 280, 607, 760, 1625, 1697, 1792, 2519
BcuI ACTAGT 1 cut(s) 734
BfmI CTRYAG 2 cut(s) 623, 1295
BfoI RGCGCY 1 cut(s) 356
BfuAI ACCTGC 1 cut(s) 142
BglII AGATCT 1 cut(s) 1942
BisI GCNGC 6 cut(s) 295, 626, 813, 1055, 2357, 2360
BlsI GCNGC 6 cut(s) 296, 627, 814, 1056, 2358, 2361
BmcAI AGTACT 1 cut(s) 1637
Bme1390I CCNGG 8 cut(s) 426, 449, 714, 832, 1020, 1382, 2030, 2078
Bme18I GGWCC 3 cut(s) 422, 1016, 1384
BmeT110I CYCGRG 2 cut(s) 869, 969
BmgBI CACGTC 1 cut(s) 1324
BmgT120I GGNCC 3 cut(s) 422, 1016, 1384
BmiI GGNNCC 2 cut(s) 84, 914
BmrFI CCNGG 8 cut(s) 426, 449, 714, 832, 1020, 1382, 2030, 2078
BmrI ACTGGG 1 cut(s) 1053
BmsI GCATC 5 cut(s) 90, 132, 1194, 1743, 2005
BmtI GCTAGC 1 cut(s) 953
BmuI ACTGGG 1 cut(s) 1053
BpiI GAAGAC 1 cut(s) 1725
BplI GAGNNNNNCTC 2 cut(s) 2389, 2421
BpmI CTGGAG 4 cut(s) 1234, 2051, 2060, 2111
Bpu10I CCTNAGC 2 cut(s) 2171, 2363
Bpu14I TTCGAA 2 cut(s) 24, 372
BpuEI CTTGAG 7 cut(s) 518, 1126, 1660, 1748, 2003, 2213, 2243
BpuMI CCSGG 3 cut(s) 714, 1020, 1382
Bsa29I ATCGAT 1 cut(s) 591
BsaBI GATNNNNATC 2 cut(s) 9, 2046
BsaHI GRCGYC 2 cut(s) 1420, 2098
BsaI GGTCTC 2 cut(s) 1625, 1697
BsaJI CCNNGG 6 cut(s) 447, 870, 1725, 1902, 2077, 2357
BsaWI WCCGGW 2 cut(s) 843, 1386
BsaXI ACNNNNNCTCC 2 cut(s) 2181, 2211
Bsc4I CCNNNNNNNGG 4 cut(s) 1854, 2044, 2411, 2495
Bse1I ACTGG 5 cut(s) 1018, 1059, 1807, 1828, 1941
Bse3DI GCAATG 1 cut(s) 2358
Bse8I GATNNNNATC 2 cut(s) 9, 2046
BseAI TCCGGA 1 cut(s) 843
BseBI CCWGG 5 cut(s) 426, 449, 832, 2030, 2078
BseCI ATCGAT 1 cut(s) 591
BseDI CCNNGG 6 cut(s) 447, 870, 1725, 1902, 2077, 2357
BseGI GGATG 3 cut(s) 10, 1131, 1335
BseJI GATNNNNATC 2 cut(s) 9, 2046
BseLI CCNNNNNNNGG 4 cut(s) 1854, 2044, 2411, 2495
BseMI GCAATG 1 cut(s) 2358
BseMII CTCAG 5 cut(s) 1131, 1509, 2162, 2310, 2387
BseNI ACTGG 5 cut(s) 1018, 1059, 1807, 1828, 1941
BseRI GAGGAG 4 cut(s) 893, 1183, 1364, 2055
BseSI GKGCMC 1 cut(s) 887
BseXI GCAGC 2 cut(s) 637, 1066
BsgI GTGCAG 1 cut(s) 514
Bsh1236I CGCG 2 cut(s) 517, 2359
Bsh1285I CGRYCG 1 cut(s) 939
BshFI GGCC 4 cut(s) 523, 815, 1399, 2240
BshVI ATCGAT 1 cut(s) 591
BsiEI CGRYCG 1 cut(s) 939
BsiHKAI GWGCWC 3 cut(s) 712, 887, 2199
BsiHKCI CYCGRG 2 cut(s) 869, 969
BsiSI CCGG 5 cut(s) 714, 844, 1019, 1382, 1387
BslI CCNNNNNNNGG 4 cut(s) 1854, 2044, 2411, 2495
BsmAI GTCTC 8 cut(s) 200, 280, 607, 760, 1625, 1697, 1792, 2519
BsmBI CGTCTC 1 cut(s) 760
BsmI GAATGC 1 cut(s) 2415
BsnI GGCC 4 cut(s) 523, 815, 1399, 2240
Bso31I GGTCTC 2 cut(s) 1625, 1697
BsoBI CYCGRG 2 cut(s) 869, 969
Bsp119I TTCGAA 2 cut(s) 24, 372
Bsp1286I GDGCHC 4 cut(s) 712, 887, 1025, 2199
Bsp13I TCCGGA 1 cut(s) 843
Bsp19I CCATGG 1 cut(s) 1725
BspANI GGCC 4 cut(s) 523, 815, 1399, 2240
BspCNI CTCAG 5 cut(s) 1132, 1510, 2163, 2311, 2388
BspDI ATCGAT 1 cut(s) 591
BspEI TCCGGA 1 cut(s) 843
BspFNI CGCG 2 cut(s) 517, 2359
BspLI GGNNCC 2 cut(s) 84, 914
BspMAI CTGCAG 1 cut(s) 627
BspMI ACCTGC 1 cut(s) 142
BspOI GCTAGC 1 cut(s) 953
BspPI GGATC 8 cut(s) 439, 590, 1012, 1077, 1660, 2052, 2055, 2454
BspQI GCTCTTC 1 cut(s) 384
BspT104I TTCGAA 2 cut(s) 24, 372
BspTNI GGTCTC 2 cut(s) 1625, 1697
BsrDI GCAATG 1 cut(s) 2358
BsrI ACTGG 5 cut(s) 1018, 1059, 1807, 1828, 1941
BssECI CCNNGG 6 cut(s) 447, 870, 1725, 1902, 2077, 2357
BssNI GRCGYC 2 cut(s) 1420, 2098
BssSI CACGAG 1 cut(s) 600
BssT1I CCWWGG 2 cut(s) 1725, 1902
Bst2BI CACGAG 1 cut(s) 600
Bst2UI CCWGG 5 cut(s) 426, 449, 832, 2030, 2078
Bst4CI ACNGT 5 cut(s) 1366, 1544, 1669, 2490, 2531
Bst6I CTCTTC 2 cut(s) 374, 384
BstACI GRCGYC 2 cut(s) 1420, 2098
BstAPI GCANNNNNTGC 1 cut(s) 553
BstBI TTCGAA 2 cut(s) 24, 372
BstC8I GCNNGC 3 cut(s) 357, 951, 1401
BstDSI CCRYGG 2 cut(s) 1725, 2357
BstF5I GGATG 3 cut(s) 10, 1131, 1335
BstFNI CGCG 2 cut(s) 517, 2359
BstH2I RGCGCY 1 cut(s) 356
BstHHI GCGC 2 cut(s) 355, 1776
BstMAI GTCTC 8 cut(s) 200, 280, 607, 760, 1625, 1697, 1792, 2519
BstMCI CGRYCG 1 cut(s) 939
BstMWI GCNNNNNNNGC 4 cut(s) 553, 932, 2218, 2368
BstNI CCWGG 5 cut(s) 426, 449, 832, 2030, 2078
BstNSI RCATGY 2 cut(s) 556, 2461
BstSCI CCNGG 8 cut(s) 424, 447, 712, 830, 1018, 1380, 2028, 2076
BstSFI CTRYAG 2 cut(s) 623, 1295
BstSLI GKGCMC 1 cut(s) 887
BstUI CGCG 2 cut(s) 517, 2359
BstV1I GCAGC 2 cut(s) 637, 1066
BstV2I GAAGAC 1 cut(s) 1725
BstX2I RGATCY 5 cut(s) 431, 1004, 1069, 1942, 2446
BstXI CCANNNNNNTGG 2 cut(s) 1066, 1258
BstYI RGATCY 5 cut(s) 431, 1004, 1069, 1942, 2446
Bsu15I ATCGAT 1 cut(s) 591
BsuRI GGCC 4 cut(s) 523, 815, 1399, 2240
BsuTUI ATCGAT 1 cut(s) 591
BtgI CCRYGG 2 cut(s) 1725, 2357
BtrI CACGTC 1 cut(s) 1324
BtsCI GGATG 3 cut(s) 10, 1131, 1335
BtsIMutI CAGTG 5 cut(s) 237, 885, 1269, 1434, 1862
BveI ACCTGC 1 cut(s) 142
Cac8I GCNNGC 3 cut(s) 357, 951, 1401
CfoI GCGC 2 cut(s) 355, 1776
Cfr13I GGNCC 3 cut(s) 422, 1016, 1384
Cfr42I CCGCGG 1 cut(s) 2360
ClaI ATCGAT 1 cut(s) 591
CpoI CGGWCCG 1 cut(s) 1384
CseI GACGC 5 cut(s) 506, 523, 1428, 2106, 2276
Csp6I GTAC 8 cut(s) 338, 1102, 1459, 1532, 1636, 1760, 1974, 2277
CspI CGGWCCG 1 cut(s) 1384
CviAII CATG 6 cut(s) 125, 553, 782, 1726, 2159, 2458
CviQI GTAC 8 cut(s) 338, 1102, 1459, 1532, 1636, 1760, 1974, 2277
EaeI YGGCCR 2 cut(s) 521, 813
Eam1104I CTCTTC 2 cut(s) 374, 384
EarI CTCTTC 2 cut(s) 374, 384
EciI GGCGGA 1 cut(s) 1821
Ecl136II GAGCTC 1 cut(s) 2197
Eco130I CCWWGG 2 cut(s) 1725, 1902
Eco147I AGGCCT 1 cut(s) 1399
Eco24I GRGCYC 2 cut(s) 1025, 2199
Eco31I GGTCTC 2 cut(s) 1625, 1697
Eco32I GATATC 1 cut(s) 76
Eco47I GGWCC 3 cut(s) 422, 1016, 1384
Eco47III AGCGCT 1 cut(s) 354
Eco53kI GAGCTC 1 cut(s) 2197
Eco57I CTGAAG 1 cut(s) 828
Eco88I CYCGRG 2 cut(s) 869, 969
EcoICRI GAGCTC 1 cut(s) 2197
EcoRI GAATTC 1 cut(s) 2326
EcoRII CCWGG 5 cut(s) 424, 447, 830, 2028, 2076
EcoRV GATATC 1 cut(s) 76
EcoT14I CCWWGG 2 cut(s) 1725, 1902
EcoT22I ATGCAT 2 cut(s) 105, 147
EcoT38I GRGCYC 2 cut(s) 1025, 2199
ErhI CCWWGG 2 cut(s) 1725, 1902
Esp3I CGTCTC 1 cut(s) 760
FaeI CATG 6 cut(s) 128, 556, 785, 1729, 2162, 2461
FatI CATG 6 cut(s) 124, 552, 781, 1725, 2158, 2457
FauI CCCGC 2 cut(s) 902, 1641
FauNDI CATATG 1 cut(s) 2052
FbaI TGATCA 1 cut(s) 1731
FblI GTMKAC 1 cut(s) 2083
Fnu4HI GCNGC 6 cut(s) 295, 626, 813, 1055, 2357, 2360
FokI GGATG 3 cut(s) 17, 1118, 1322
FriOI GRGCYC 2 cut(s) 1025, 2199
Fsp4HI GCNGC 6 cut(s) 295, 626, 813, 1055, 2357, 2360
GlaI GCGC 2 cut(s) 354, 1775
GluI GCNGC 6 cut(s) 295, 626, 813, 1055, 2357, 2360
GsuI CTGGAG 4 cut(s) 1234, 2051, 2060, 2111
HaeII RGCGCY 1 cut(s) 356
HaeIII GGCC 4 cut(s) 523, 815, 1399, 2240
HapII CCGG 5 cut(s) 714, 844, 1019, 1382, 1387
HgaI GACGC 5 cut(s) 506, 523, 1428, 2106, 2276
HhaI GCGC 2 cut(s) 355, 1776
Hin1I GRCGYC 2 cut(s) 1420, 2098
Hin1II CATG 6 cut(s) 128, 556, 785, 1729, 2162, 2461
Hin6I GCGC 2 cut(s) 353, 1774
HinP1I GCGC 2 cut(s) 353, 1774
HincII GTYRAC 2 cut(s) 133, 2185
HindII GTYRAC 2 cut(s) 133, 2185
HindIII AAGCTT 1 cut(s) 1836
HinfI GANTC 9 cut(s) 275, 820, 847, 1373, 1474, 1642, 1768, 2122, 2392
HpaI GTTAAC 1 cut(s) 2185
HpaII CCGG 5 cut(s) 714, 844, 1019, 1382, 1387
HphI GGTGA 5 cut(s) 294, 795, 820, 1741, 1885
Hpy188I TCNGA 9 cut(s) 251, 583, 1077, 1378, 1519, 2167, 2391, 2397, 2494
Hpy99I CGWCG 3 cut(s) 522, 1422, 2348
HpyAV CCTTC 8 cut(s) 20, 466, 829, 1163, 1204, 1582, 2087, 2099
HpyCH4III ACNGT 5 cut(s) 1366, 1544, 1669, 2490, 2531
HpyCH4IV ACGT 3 cut(s) 1183, 1323, 2181
HpyF10VI GCNNNNNNNGC 4 cut(s) 553, 932, 2218, 2368
HpySE526I ACGT 3 cut(s) 1183, 1323, 2181
Hsp92I GRCGYC 2 cut(s) 1420, 2098
Hsp92II CATG 6 cut(s) 128, 556, 785, 1729, 2162, 2461
HspAI GCGC 2 cut(s) 353, 1774
Kpn2I TCCGGA 1 cut(s) 843
Ksp22I TGATCA 1 cut(s) 1731
KspAI GTTAAC 1 cut(s) 2185
KspI CCGCGG 1 cut(s) 2360
LguI GCTCTTC 1 cut(s) 384
LmnI GCTCC 5 cut(s) 715, 982, 2202, 2348, 2368
Lsp1109I GCAGC 2 cut(s) 637, 1066
LweI GCATC 5 cut(s) 90, 132, 1194, 1743, 2005
MaeII ACGT 3 cut(s) 1183, 1323, 2181
MaeIII GTNAC 7 cut(s) 187, 511, 696, 801, 1360, 1576, 1860
MboII GAAGA 9 cut(s) 244, 361, 401, 596, 890, 1064, 1730, 1836, 2158
MfeI CAATTG 1 cut(s) 2477
MflI RGATCY 5 cut(s) 431, 1004, 1069, 1942, 2446
MhlI GDGCHC 4 cut(s) 712, 887, 1025, 2199
MluI ACGCGT 1 cut(s) 515
MlyI GAGTC 3 cut(s) 269, 1382, 1651
Mph1103I ATGCAT 2 cut(s) 105, 147
MroI TCCGGA 1 cut(s) 843
MroXI GAANNNNTTC 1 cut(s) 824
MseI TTAA 9 cut(s) 993, 1086, 1148, 1433, 1614, 1833, 1869, 2184, 2300
MslI CAYNNNNRTG 3 cut(s) 123, 1326, 1727
MspA1I CMGCKG 3 cut(s) 935, 2273, 2359
MspI CCGG 5 cut(s) 714, 844, 1019, 1382, 1387
MspR9I CCNGG 8 cut(s) 426, 449, 714, 832, 1020, 1382, 2030, 2078
MunI CAATTG 1 cut(s) 2477
Mva1269I GAATGC 1 cut(s) 2415
MvaI CCWGG 5 cut(s) 426, 449, 832, 2030, 2078
MvnI CGCG 2 cut(s) 517, 2359
MwoI GCNNNNNNNGC 4 cut(s) 553, 932, 2218, 2368
NciI CCSGG 3 cut(s) 714, 1020, 1382
NcoI CCATGG 1 cut(s) 1725
NdeI CATATG 1 cut(s) 2052
NheI GCTAGC 1 cut(s) 949
NlaIII CATG 6 cut(s) 128, 556, 785, 1729, 2162, 2461
NlaIV GGNNCC 2 cut(s) 84, 914
NmuCI GTSAC 4 cut(s) 187, 511, 801, 1860
NsiI ATGCAT 2 cut(s) 105, 147
NspI RCATGY 2 cut(s) 556, 2461
NspV TTCGAA 2 cut(s) 24, 372
OliI CACNNNNGTG 2 cut(s) 1326, 1727
PceI AGGCCT 1 cut(s) 1399
PciSI GCTCTTC 1 cut(s) 384
PcsI WCGNNNNNNNCGW 1 cut(s) 514
PctI GAATGC 1 cut(s) 2415
PdmI GAANNNNTTC 1 cut(s) 824
PfeI GAWTC 6 cut(s) 820, 847, 1474, 1768, 2122, 2392
PfoI TCCNGGA 1 cut(s) 712
PkrI GCNGC 6 cut(s) 296, 627, 814, 1056, 2358, 2361
PleI GAGTC 3 cut(s) 269, 1381, 1650
PpsI GAGTC 3 cut(s) 269, 1381, 1650
Psp124BI GAGCTC 1 cut(s) 2199
Psp1406I AACGTT 2 cut(s) 1183, 2181
Psp6I CCWGG 5 cut(s) 424, 447, 830, 2028, 2076
PspGI CCWGG 5 cut(s) 424, 447, 830, 2028, 2076
PspN4I GGNNCC 2 cut(s) 84, 914
PspPI GGNCC 3 cut(s) 422, 1016, 1384
PstI CTGCAG 1 cut(s) 627
PsuI RGATCY 5 cut(s) 431, 1004, 1069, 1942, 2446
PvuII CAGCTG 1 cut(s) 2273
RsaI GTAC 8 cut(s) 339, 1103, 1460, 1533, 1637, 1761, 1975, 2278
RsaNI GTAC 8 cut(s) 338, 1102, 1459, 1532, 1636, 1760, 1974, 2277
RseI CAYNNNNRTG 3 cut(s) 123, 1326, 1727
Rsr2I CGGWCCG 1 cut(s) 1384
RsrII CGGWCCG 1 cut(s) 1384
SacI GAGCTC 1 cut(s) 2199
SacII CCGCGG 1 cut(s) 2360
SapI GCTCTTC 1 cut(s) 384
SaqAI TTAA 9 cut(s) 993, 1086, 1148, 1433, 1614, 1833, 1869, 2184, 2300
SatI GCNGC 6 cut(s) 295, 626, 813, 1055, 2357, 2360
Sau96I GGNCC 3 cut(s) 422, 1016, 1384
ScaI AGTACT 1 cut(s) 1637
SchI GAGTC 3 cut(s) 269, 1382, 1651
ScrFI CCNGG 8 cut(s) 426, 449, 714, 832, 1020, 1382, 2030, 2078
SduI GDGCHC 4 cut(s) 712, 887, 1025, 2199
SfaNI GCATC 5 cut(s) 90, 132, 1194, 1743, 2005
SfcI CTRYAG 2 cut(s) 623, 1295
Sfr303I CCGCGG 1 cut(s) 2360
SfuI TTCGAA 2 cut(s) 24, 372
SgrBI CCGCGG 1 cut(s) 2360
SinI GGWCC 3 cut(s) 422, 1016, 1384
SmiMI CAYNNNNRTG 3 cut(s) 123, 1326, 1727
SmlI CTYRAG 7 cut(s) 533, 1105, 1639, 1763, 1982, 2192, 2222
SmoI CTYRAG 7 cut(s) 533, 1105, 1639, 1763, 1982, 2192, 2222
SpeI ACTAGT 1 cut(s) 734
SseBI AGGCCT 1 cut(s) 1399
SspI AATATT 2 cut(s) 1550, 1831
SstI GAGCTC 1 cut(s) 2199
StuI AGGCCT 1 cut(s) 1399
StyD4I CCNGG 8 cut(s) 424, 447, 712, 830, 1018, 1380, 2028, 2076
StyI CCWWGG 2 cut(s) 1725, 1902
TaaI ACNGT 5 cut(s) 1366, 1544, 1669, 2490, 2531
TaiI ACGT 3 cut(s) 1186, 1326, 2184
TaqI TCGA 8 cut(s) 18, 24, 284, 372, 591, 1417, 1820, 2003
TaqII GACCGA 1 cut(s) 410
TatI WGTACW 6 cut(s) 337, 1101, 1458, 1635, 1759, 1973
TauI GCSGC 4 cut(s) 297, 815, 2359, 2362
TfiI GAWTC 6 cut(s) 820, 847, 1474, 1768, 2122, 2392
Tru1I TTAA 9 cut(s) 993, 1086, 1148, 1433, 1614, 1833, 1869, 2184, 2300
Tru9I TTAA 9 cut(s) 993, 1086, 1148, 1433, 1614, 1833, 1869, 2184, 2300
TscAI CASTG 5 cut(s) 244, 892, 1276, 1434, 1869
TseFI GTSAC 4 cut(s) 187, 511, 801, 1860
TseI GCWGC 2 cut(s) 625, 1054
Tsp45I GTSAC 4 cut(s) 187, 511, 801, 1860
TspDTI ATGAA 7 cut(s) 54, 182, 402, 1254, 1787, 2006, 2325
TspGWI ACGGA 2 cut(s) 1316, 2361
TspRI CASTG 5 cut(s) 244, 892, 1276, 1434, 1869
VneI GTGCAC 1 cut(s) 883
VpaK11BI GGWCC 3 cut(s) 422, 1016, 1384
XbaI TCTAGA 2 cut(s) 2140, 2381
XceI RCATGY 2 cut(s) 556, 2461
XmiI GTMKAC 1 cut(s) 2083
XmnI GAANNNNTTC 1 cut(s) 824
ZrmI AGTACT 1 cut(s) 1637
Zsp2I ATGCAT 2 cut(s) 105, 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.