Rh5DG195100

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000865
Physical Location & Seq
Reverse (-)
21623241 .. 21624570
1330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG195100.1

Sequence Viewer

Length: 927 bp
ATGGCCGCGGATATTGCCGTTTCAGCAGCATTGGCATTAGTTGAGAAGGTCGCAAGCTTCGTCGACAAAATACGCAAAAGTCCGAGCCATGTCCGTGAAGCTCTTGATAAGGCAAGAACTTGTTTGGAGAGGATGGATGCCTATCTAACGACGTCCCAAGCACGGATTGATCAAGGCAATCAAGTGCAGCTTCAAGCTCGTGTTAAACAGGTCCGAGATGTAGTTCATTACATTGAGGATGCACTTGACGAGTTCACAATTCAGGTTCCCCATCATTCCCAGTATACCCATTGGTATTCTGGGATGACACGCTGCGCTGCTCATTCCACGTCTTATTGGTTAGCAAGTAACAGATTGTCCTCCGAAATTGAAGTTATTATTACAAATAAGATCGGTTTTCTTAGAGAACTCGATGGACTCACACAAGAAGGCCAACCAACTTGTAGCACAAGCCAGCAGCATGGAGGTACTTTGACCCCGCAAGTTCTTGAAGACGATGAAATAGTGGGCTTTGACGTGCCTAAACAAAAACTCATTGAACAGTTGATGAAAGGAGATCCAAAGCGTCTGACGATTTCCATTGTAGGTCCTGGCGGCTCCGGCAAAACCACTATCATGAGGAATGTTTACGACACTAAAAAGGTTCAAAGGAATTTTAATTGCCGAGCTTGGATCGATGTGTTGCGTCCTCTAAATCTCGAAAATGTGTTGCGCACCATGTTGAGCAGTTTTGATCCAAAAGGAAAACGGCAGGAACCAGAAGGAACAATTAATTGTCTAAAAGAAAAATTGAAACTACTTCTGCAGAAAAAGAAGTTTCTGGTTGTCTTGGATAATGTTTGGCGTAACGAGGATTTGGAGTCCATTGTAAATGCACTGCCCAATGGTTCCCCTGGGAGTAAAATACTCCTGTTGGTGCCGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

308

Amino Acids

34.59

Weight (kDa)

8.7

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 94 1.3e-11 Rx N-terminal domain
NB-ARC PF00931 174 - 304 1.9e-27 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 155
Acc16I TGCGCA 1 cut(s) 713
AccB1I GGYRCC 1 cut(s) 916
AccI GTMKAC 2 cut(s) 63, 284
AccII CGCG 1 cut(s) 8
AciI CCGC 4 cut(s) 6, 8, 479, 594
AclWI GGATC 3 cut(s) 551, 680, 728
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 652
AcyI GRCGYC 1 cut(s) 152
AfaI GTAC 1 cut(s) 469
AfiI CCNNNNNNNGG 1 cut(s) 162
AgsI TTSAA 6 cut(s) 194, 371, 491, 539, 647, 793
AjiI CACGTC 2 cut(s) 330, 517
AjnI CCWGG 2 cut(s) 589, 892
AluBI AGCT 5 cut(s) 57, 101, 190, 197, 668
AluI AGCT 5 cut(s) 57, 101, 190, 197, 668
AlwI GGATC 3 cut(s) 551, 680, 728
AoxI GGCC 2 cut(s) 3, 430
ApeKI GCWGC 5 cut(s) 26, 187, 312, 317, 457
ApoI RAATTY 1 cut(s) 652
AseI ATTAAT 1 cut(s) 771
AspLEI GCGC 2 cut(s) 317, 714
AspS9I GGNCC 2 cut(s) 211, 587
AvaII GGWCC 2 cut(s) 211, 587
BanI GGYRCC 1 cut(s) 916
BauI CACGAG 1 cut(s) 198
BbsI GAAGAC 1 cut(s) 498
BbvI GCAGC 5 cut(s) 38, 199, 299, 304, 469
BccI CCATC 3 cut(s) 127, 279, 407
BceAI ACGGC 2 cut(s) 2, 764
BciT130I CCWGG 2 cut(s) 591, 894
BclI TGATCA 1 cut(s) 169
BfaI CTAG 1 cut(s) 925
BfmI CTRYAG 1 cut(s) 803
BisI GCNGC 7 cut(s) 6, 27, 188, 313, 318, 458, 595
BlsI GCNGC 7 cut(s) 7, 28, 189, 314, 319, 459, 596
Bme1390I CCNGG 2 cut(s) 591, 894
Bme18I GGWCC 2 cut(s) 211, 587
BmgBI CACGTC 2 cut(s) 330, 517
BmgT120I GGNCC 2 cut(s) 211, 587
BmiI GGNNCC 5 cut(s) 267, 598, 756, 889, 918
BmrFI CCNGG 2 cut(s) 591, 894
BmrI ACTGGG 1 cut(s) 274
BmsI GCATC 2 cut(s) 127, 229
BmuI ACTGGG 1 cut(s) 274
BpiI GAAGAC 1 cut(s) 498
Bsa29I ATCGAT 1 cut(s) 675
BsaBI GATNNNNATC 1 cut(s) 141
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 3 cut(s) 6, 892, 893
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse1I ACTGG 1 cut(s) 280
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 2 cut(s) 591, 894
BseCI ATCGAT 1 cut(s) 675
BseDI CCNNGG 3 cut(s) 6, 892, 893
BseGI GGATG 4 cut(s) 138, 142, 244, 309
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 1 cut(s) 162
BseNI ACTGG 1 cut(s) 280
BseXI GCAGC 5 cut(s) 38, 199, 299, 304, 469
BsgI GTGCAG 1 cut(s) 206
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 2 cut(s) 5, 432
BshNI GGYRCC 1 cut(s) 916
BshVI ATCGAT 1 cut(s) 675
BsiSI CCGG 1 cut(s) 600
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 1 cut(s) 162
BsmFI GGGAC 1 cut(s) 139
BsnI GGCC 2 cut(s) 5, 432
Bsp143I GATC 6 cut(s) 169, 390, 556, 672, 733, 921
BspACI CCGC 4 cut(s) 6, 8, 479, 594
BspANI GGCC 2 cut(s) 5, 432
BspDI ATCGAT 1 cut(s) 675
BspFNI CGCG 1 cut(s) 8
BspHI TCATGA 1 cut(s) 615
BspLI GGNNCC 5 cut(s) 267, 598, 756, 889, 918
BspMAI CTGCAG 1 cut(s) 807
BspPI GGATC 3 cut(s) 551, 680, 728
BspT107I GGYRCC 1 cut(s) 916
BsrI ACTGG 1 cut(s) 280
BssECI CCNNGG 3 cut(s) 6, 892, 893
BssMI GATC 6 cut(s) 169, 390, 556, 672, 733, 921
BssNAI GTATAC 1 cut(s) 285
BssNI GRCGYC 1 cut(s) 152
BssSI CACGAG 1 cut(s) 198
Bst1107I GTATAC 1 cut(s) 285
Bst2BI CACGAG 1 cut(s) 198
Bst2UI CCWGG 2 cut(s) 591, 894
Bst4CI ACNGT 1 cut(s) 543
BstACI GRCGYC 1 cut(s) 152
BstC8I GCNNGC 2 cut(s) 55, 455
BstDEI CTNAG 1 cut(s) 401
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 4 cut(s) 138, 142, 244, 309
BstFNI CGCG 1 cut(s) 8
BstHHI GCGC 2 cut(s) 317, 714
BstKTI GATC 6 cut(s) 172, 393, 559, 675, 736, 924
BstMBI GATC 6 cut(s) 169, 390, 556, 672, 733, 921
BstMWI GCNNNNNNNGC 4 cut(s) 14, 23, 32, 600
BstNI CCWGG 2 cut(s) 591, 894
BstSCI CCNGG 2 cut(s) 589, 892
BstSFI CTRYAG 1 cut(s) 803
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 5 cut(s) 38, 199, 299, 304, 469
BstV2I GAAGAC 1 cut(s) 498
BstX2I RGATCY 1 cut(s) 556
BstXI CCANNNNNNTGG 1 cut(s) 461
BstYI RGATCY 1 cut(s) 556
BstZ17I GTATAC 1 cut(s) 285
Bsu15I ATCGAT 1 cut(s) 675
BsuRI GGCC 2 cut(s) 5, 432
BsuTUI ATCGAT 1 cut(s) 675
BtgI CCRYGG 1 cut(s) 6
BtrI CACGTC 2 cut(s) 330, 517
BtsCI GGATG 4 cut(s) 138, 142, 244, 309
BtsI GCAGTG 1 cut(s) 875
BtsIMutI CAGTG 1 cut(s) 875
Cac8I GCNNGC 2 cut(s) 55, 455
CciI TCATGA 1 cut(s) 615
CfoI GCGC 2 cut(s) 317, 714
Cfr13I GGNCC 2 cut(s) 211, 587
Cfr42I CCGCGG 1 cut(s) 9
ClaI ATCGAT 1 cut(s) 675
CseI GACGC 2 cut(s) 554, 674
Csp6I GTAC 1 cut(s) 468
CviAII CATG 4 cut(s) 89, 461, 616, 718
CviQI GTAC 1 cut(s) 468
DdeI CTNAG 1 cut(s) 401
DpnI GATC 6 cut(s) 171, 392, 558, 674, 735, 923
DpnII GATC 6 cut(s) 169, 390, 556, 672, 733, 921
EaeI YGGCCR 1 cut(s) 3
Eco47I GGWCC 2 cut(s) 211, 587
EcoO109I RGGNCCY 1 cut(s) 587
EcoRII CCWGG 2 cut(s) 589, 892
FaeI CATG 4 cut(s) 92, 464, 619, 721
FaiI YATR 5 cut(s) 90, 285, 462, 617, 719
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FaqI GGGAC 1 cut(s) 139
FatI CATG 4 cut(s) 88, 460, 615, 717
FauI CCCGC 1 cut(s) 486
FbaI TGATCA 1 cut(s) 169
FblI GTMKAC 2 cut(s) 63, 284
Fnu4HI GCNGC 7 cut(s) 6, 27, 188, 313, 318, 458, 595
FokI GGATG 4 cut(s) 145, 149, 251, 316
Fsp4HI GCNGC 7 cut(s) 6, 27, 188, 313, 318, 458, 595
FspBI CTAG 1 cut(s) 925
FspI TGCGCA 1 cut(s) 713
GlaI GCGC 2 cut(s) 316, 713
GluI GCNGC 7 cut(s) 6, 27, 188, 313, 318, 458, 595
HaeIII GGCC 2 cut(s) 5, 432
HapII CCGG 1 cut(s) 600
HgaI GACGC 2 cut(s) 554, 674
HhaI GCGC 2 cut(s) 317, 714
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 4 cut(s) 92, 464, 619, 721
Hin6I GCGC 2 cut(s) 315, 712
HinP1I GCGC 2 cut(s) 315, 712
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HindIII AAGCTT 1 cut(s) 55
HinfI GANTC 2 cut(s) 417, 860
HpaII CCGG 1 cut(s) 600
Hpy166II GTNNAC 4 cut(s) 64, 255, 285, 628
Hpy188I TCNGA 4 cut(s) 84, 215, 364, 570
Hpy188III TCNNGA 4 cut(s) 104, 488, 616, 698
Hpy8I GTNNAC 4 cut(s) 64, 255, 285, 628
Hpy99I CGWCG 2 cut(s) 65, 154
HpyAV CCTTC 3 cut(s) 40, 422, 755
HpyCH4III ACNGT 1 cut(s) 543
HpyCH4IV ACGT 3 cut(s) 152, 329, 516
HpyCH4V TGCA 4 cut(s) 187, 242, 805, 875
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 23, 32, 600
HpyF3I CTNAG 1 cut(s) 401
HpySE526I ACGT 3 cut(s) 152, 329, 516
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 4 cut(s) 92, 464, 619, 721
HspAI GCGC 2 cut(s) 315, 712
Ksp22I TGATCA 1 cut(s) 169
KspI CCGCGG 1 cut(s) 9
Kzo9I GATC 6 cut(s) 169, 390, 556, 672, 733, 921
LmnI GCTCC 1 cut(s) 602
Lsp1109I GCAGC 5 cut(s) 38, 199, 299, 304, 469
LweI GCATC 2 cut(s) 127, 229
MaeI CTAG 1 cut(s) 925
MaeII ACGT 3 cut(s) 152, 329, 516
MaeIII GTNAC 2 cut(s) 347, 845
MalI GATC 6 cut(s) 171, 392, 558, 674, 735, 923
MboI GATC 6 cut(s) 169, 390, 556, 672, 733, 921
MboII GAAGA 1 cut(s) 503
MflI RGATCY 1 cut(s) 556
MluCI AATT 7 cut(s) 258, 366, 652, 658, 768, 772, 788
MlyI GAGTC 2 cut(s) 411, 869
MnlI CCTC 7 cut(s) 123, 229, 370, 458, 612, 699, 844
MseI TTAA 3 cut(s) 204, 657, 771
MslI CAYNNNNRTG 2 cut(s) 93, 614
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 600
MspR9I CCNGG 2 cut(s) 591, 894
MvaI CCWGG 2 cut(s) 591, 894
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 4 cut(s) 14, 23, 32, 600
NdeII GATC 6 cut(s) 169, 390, 556, 672, 733, 921
NlaIII CATG 4 cut(s) 92, 464, 619, 721
NlaIV GGNNCC 5 cut(s) 267, 598, 756, 889, 918
NmeAIII GCCGAG 1 cut(s) 689
NsbI TGCGCA 1 cut(s) 713
PagI TCATGA 1 cut(s) 615
PasI CCCWGGG 1 cut(s) 893
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PkrI GCNGC 7 cut(s) 7, 28, 189, 314, 319, 459, 596
PleI GAGTC 2 cut(s) 411, 868
PpsI GAGTC 2 cut(s) 411, 868
PpuMI RGGWCCY 1 cut(s) 587
PshBI ATTAAT 1 cut(s) 771
Psp5II RGGWCCY 1 cut(s) 587
Psp6I CCWGG 2 cut(s) 589, 892
PspGI CCWGG 2 cut(s) 589, 892
PspN4I GGNNCC 5 cut(s) 267, 598, 756, 889, 918
PspPI GGNCC 2 cut(s) 211, 587
PspPPI RGGWCCY 1 cut(s) 587
PstI CTGCAG 1 cut(s) 807
PsuI RGATCY 1 cut(s) 556
RsaI GTAC 1 cut(s) 469
RsaNI GTAC 1 cut(s) 468
RseI CAYNNNNRTG 2 cut(s) 93, 614
SacII CCGCGG 1 cut(s) 9
SalI GTCGAC 1 cut(s) 62
SaqAI TTAA 3 cut(s) 204, 657, 771
SatI GCNGC 7 cut(s) 6, 27, 188, 313, 318, 458, 595
Sau3AI GATC 6 cut(s) 169, 390, 556, 672, 733, 921
Sau96I GGNCC 2 cut(s) 211, 587
SchI GAGTC 2 cut(s) 411, 869
ScrFI CCNGG 2 cut(s) 591, 894
SfaNI GCATC 2 cut(s) 127, 229
SfcI CTRYAG 1 cut(s) 803
Sfr303I CCGCGG 1 cut(s) 9
SgrBI CCGCGG 1 cut(s) 9
SinI GGWCC 2 cut(s) 211, 587
SmiMI CAYNNNNRTG 2 cut(s) 93, 614
Sse9I AATT 7 cut(s) 258, 366, 652, 658, 768, 772, 788
SsiI CCGC 4 cut(s) 6, 8, 479, 594
SspMI CTAG 1 cut(s) 925
StyD4I CCNGG 2 cut(s) 589, 892
TaaI ACNGT 1 cut(s) 543
TaiI ACGT 3 cut(s) 155, 332, 519
TaqI TCGA 4 cut(s) 63, 411, 675, 699
TasI AATT 7 cut(s) 258, 366, 652, 658, 768, 772, 788
TauI GCSGC 2 cut(s) 8, 597
Tru1I TTAA 3 cut(s) 204, 657, 771
Tru9I TTAA 3 cut(s) 204, 657, 771
TscAI CASTG 1 cut(s) 882
TseI GCWGC 5 cut(s) 26, 187, 312, 317, 457
TspDTI ATGAA 3 cut(s) 215, 513, 563
TspGWI ACGGA 2 cut(s) 83, 178
TspRI CASTG 1 cut(s) 882
VpaK11BI GGWCC 2 cut(s) 211, 587
VspI ATTAAT 1 cut(s) 771
XapI RAATTY 1 cut(s) 652
XcmI CCANNNNNNNNNTGG 1 cut(s) 296
XmiI GTMKAC 2 cut(s) 63, 284
XspI CTAG 1 cut(s) 925
ZraI GACGTC 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.